CN115282342A - Medical implant coating, preparation method thereof and medical implant - Google Patents
Medical implant coating, preparation method thereof and medical implant Download PDFInfo
- Publication number
- CN115282342A CN115282342A CN202210927363.4A CN202210927363A CN115282342A CN 115282342 A CN115282342 A CN 115282342A CN 202210927363 A CN202210927363 A CN 202210927363A CN 115282342 A CN115282342 A CN 115282342A
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- CN
- China
- Prior art keywords
- medical implant
- coating
- solution
- malic acid
- octanediol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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Abstract
The invention discloses a medical implant coating and a preparation method thereof, wherein the preparation method comprises the following steps: s10, preparing a buffer solution, and dissolving tannic acid and silver nitrate in the buffer solution respectively to obtain a tannic acid solution and a silver nitrate solution; s20, depositing the medical implant substrate in a tannic acid solution and a silver nitrate solution respectively and sequentially in a circulating manner to and fro, and forming a plurality of tannic acid-silver nanoparticle coatings on the surface of the medical implant substrate; s30, soaking the medical implant substrate with the plurality of layers of tannic acid-silver nanoparticle coatings formed on the surface in a malic acid-1, 8-octanediol polymer solution, taking out the medical implant substrate, performing a thermal crosslinking polymerization reaction, and preparing the tannic acid-silver nanoparticle-malic acid polymer coating, namely the medical implant coating, on the surface of the medical implant substrate. The preparation method is simple, and the prepared medical implant coating has good antibacterial stability and long-acting property.
Description
Technical Field
The invention relates to the technical field of high polymer materials and implants, in particular to a preparation method of a medical implant coating capable of realizing long-acting slow-release antibacterial and anti-inflammatory effects, a medical implant coating prepared by the preparation method and an implantable medical implant with the medical implant coating.
Background
In recent years, medical implants and implantation devices have become more widely used in clinical practice. At the same time, post-implantation infection associated with the implant is one of the major risks faced. In particular, for semi-implants that are exposed to the outside of the body surface, the surface of the implant and the spaces between the implant and the surrounding tissue are very vulnerable to bacterial attack from the wound during the period from the completion of the implantation to the healing of the wound around the implant, and if the surface of the implant does not have sufficient antimicrobial properties, the bacteria can easily adhere irreversibly to the surface of the implant and rapidly secrete resistant biofilms (mainly consisting of fibrin, polysaccharide matrix, etc.). On the one hand, the bacterial biofilm has the capacity to resist and escape antibacterial drugs and the immune system of a host, and on the other hand, the biofilm provides shelter and a inhabitation place for bacteria, and after the biofilm is cracked, the bacteria can be released to spread to further infect the body. In order to solve the infection problem of the implant, antibiotics are often used for preventing and treating the implant clinically. However, the use of antibiotics has the disadvantages of bacterial resistance and poor antibiotic efficacy due to the presence of a biofilm. Therefore, under the large background, the multifunctional coating which has good biocompatibility, can slowly release the antibacterial and anti-inflammatory effects for a long time and has good biocompatibility is endowed to the surface of the implant, and the multifunctional coating has important clinical significance for clinical application of the implant.
Metal-polyphenol Networks (Metal-polyphenol Networks) are emerging organic-inorganic hybrid systems which are emerging in recent years and mainly comprise plant and fruit-derived polyphenol compounds and various Metal ions. The metal-polyphenol system has the outstanding characteristics and advantages that on one hand, the metal-polyphenol system has the widely reported functions of resisting bacteria, oxidation, tumors, bleeding and the like of polyphenol compounds, and on the other hand, the metal-polyphenol system has the broad-spectrum antibacterial property of metal ions. The metal-polyphenol system is widely applied to wound repair, bone regeneration repair and medical appliances as an antibacterial and anti-inflammatory complex system. It is worth mentioning that tannin-inspired polyphenolic compounds are rich in catechol and pyrogallol groups, similar to mussel-inspired catechol-rich dopa adhesion strategies, and the rich phenolic hydroxyl groups enable tannic acid to form covalent and non-covalent bonds with substrates of various materials, and the resulting non-specific binding forces enable tannic acid to form reactive precursor base coats on the substrate material surfaces. The method provides theoretical basis for constructing a metal-polyphenol film network on the surface of the artificial hair fiber for antibacterial and anti-inflammatory functional modification. Further, a period of 2 to 4 weeks or more is often required after implant until wound healing and restoration to homeostasis around the implant. Previous coatings often failed to sustain long term release due to burst release of antimicrobial substances, and layer-by-layer self-assembled metal-polyphenol networks, while effective at increasing loading and sustained release duration, also suffer from this problem.
Disclosure of Invention
The invention aims to provide a preparation method of a medical implant coating capable of slowly releasing bacteria and resisting inflammation for a long time, the preparation method is simple, and the prepared medical implant coating has good antibacterial stability and long-acting property.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of a medical implant coating is provided, which comprises the following steps:
s10, preparing a buffer solution, and dissolving tannic acid and silver nitrate in the buffer solution respectively to obtain a tannic acid solution and a silver nitrate solution;
s20, respectively and sequentially circularly depositing the medical implant substrate in a tannic acid solution and a silver nitrate solution to and fro, and forming a plurality of tannic acid-silver nanoparticle coatings on the surface of the medical implant substrate;
s30, soaking the medical implant substrate with the plurality of layers of tannic acid-silver nanoparticle coatings formed on the surface in a malic acid-1, 8-octanediol polymer solution, taking out the medical implant substrate, performing a thermal crosslinking polymerization reaction, and preparing the tannic acid-silver nanoparticle-malic acid polymer coating, namely the medical implant coating, on the surface of the medical implant substrate.
Wherein the antibacterial ability of the medical implant coating comes from silver nanoparticles reduced by polyphenol, and the polyphenol is preferentially deposited on the surface of the medical implant substrate as a precursor reaction coating and shows the characteristics of scavenging free radicals and resisting inflammation. The addition of the polymalic acid on the outermost layer further optimizes the biocompatibility of the medical implant coating and releases the silver ions in a sustained manner, thereby prolonging the antibacterial aging.
The application encapsulates a biodegradable polymalic acid coating outside a metal-polyphenol antibacterial coating, and malic acid and tannic acid are crosslinked under a heating condition to form a tannic acid-silver nanoparticle-polymalic acid coating. The composite coating can show good biocompatibility and anti-inflammatory characteristic on the premise of not influencing the antibacterial efficiency obviously, and the antibacterial time limit of the coating is greatly prolonged by releasing the slow-release silver ions.
In the invention, tris-NO is adopted as the buffer solution 3 Buffered solutions (pH = 8.5), mainly composed of Tris-base solution and HNO 3 Is configured to form.
Wherein the concentration of the malic acid-1, 8 octanediol polymer solution was 10%.
As a preferable embodiment of the preparation method of the medical implant coating, in step S10, the concentration of the tannic acid solution is 1-20mg/ml, and the concentration of the silver nitrate solution is 0.01-0.2mg/ml.
As a preferable scheme of the preparation method of the medical implant coating, the step S20 specifically comprises the following steps:
s20a, soaking the medical implant substrate in a tannic acid solution, uniformly stirring for 12 hours, and depositing a first layer of tannic acid on the surface of the medical implant substrate;
s20b, taking out the medical implant substrate, rinsing the medical implant substrate by adopting a buffer solution, and then soaking the medical implant substrate in a silver nitrate solution under a light-proof condition to obtain a basic tannin-silver nanoparticle coating;
and S20c, repeating the steps S20a and S20b, circularly soaking for 5 cycles, and preparing the tannin-silver nanoparticles in a layer-by-layer self-assembly manner on the surface of the medical implant substrate.
As a preferable scheme of the preparation method of the medical implant coating, from the second soaking, the soaking time of the medical implant substrate in the tannic acid solution is 10min each time; the soaking time of the medical implant substrate in the silver nitrate solution is 10min each time.
As a preferable embodiment of the method for preparing the coating layer of the medical implant, the method for preparing the malic acid-1, 8 octanediol polymer solution in step S30 comprises the steps of:
adding L-malic acid powder and 1, 8-octanediol powder into a reaction vessel in a mass ratio of 1;
after the malic acid and the 1, 8-octanediol are completely dissolved, reducing the temperature to 120 ℃ and uniformly stirring to perform esterification reaction on the malic acid and the 1, 8-octanediol;
after the esterification reaction of malic acid and 1, 8-octanediol is carried out for 18-24h, adding a solvent to dissolve a reaction product;
after the solvent is completely volatilized, cleaning and vacuum-drying the reaction product to obtain malic acid-1, 8 octanediol polymer;
the malic acid-1, 8 octanediol polymer was dissolved in 6-fluoroisopropanol to prepare a malic acid-1, 8 octanediol polymer solution.
As a preferable scheme of the preparation method of the medical implant coating, the mass ratio of the malic acid-1, 8-octanediol polymer to the 6-fluoroisopropanol is 1.
As a preferable scheme of the preparation method of the medical implant coating, the medical implant substrate with a plurality of layers of tannin-silver nanoparticle coatings formed on the surface thereof soaked in the malic acid-1, 8 octanediol polymer solution is dried;
drying, heating in a vacuum drying oven at 120 deg.C for crosslinking polymerization reaction for 3-5 days to obtain tannin-silver nanoparticle-malic acid polymer coating on the surface of the medical implant substrate, i.e. medical implant coating.
As a preferable scheme of the preparation method of the medical implant coating, the medical implant substrate with a plurality of layers of tannin-silver nanoparticle coatings formed on the surface is soaked in the malic acid-1, 8 octanediol polymer solution for 1-2s.
The invention also aims to provide a medical implant coating which is prepared by the preparation method.
It is still another object of the present invention to provide a medical implant comprising a medical implant substrate and a tannin-silver nanoparticles-malic acid polymer coating deposited on the surface of the medical implant substrate.
The medical implant substrate comprises artificial hair fibers, medical instruments such as medical catheters, intravenous catheters and syringe needles. The invention can form a long-acting antibacterial and anti-inflammatory functional coating on the surface of the medical implant substrate, and has potential application value in the surface functional modification of clinical implants.
The invention has the beneficial effects that: the tannin-silver nanoparticle coating based on the metal-polyphenol system is generated by quickly reacting the tannin with the silver nitrate solution in a layer-by-layer self-assembly mode. Subsequently, a malic acid-1, 8-octanediol polymer obtained by reacting 1, 8-octanediol with L-malic acid was wrapped around the outer layer of the tannic acid-silver nanoparticles and heat-crosslinked. The phenolic hydroxyl groups of the tannic acid crosslink with the carboxyl groups of the polymalic acid, so that the silver nanoparticles are further stabilized in the composite coating. On one hand, the tannin-silver nanoparticles provide excellent antibacterial ability and anti-inflammatory property, on the other hand, the addition of the polymalic acid further improves the biocompatibility of the coating, obviously prolongs the antibacterial aging, and can be used as an effective functional coating to be applied to the surface of a medical implant substrate.
Drawings
Figure 1 is an SEM image of a TAP coating deposited on an artificial hair fiber.
Figure 2 is an SEM image of TAP coating deposited on a polyamide membrane sheet.
Fig. 3 is a graph of water contact angles for coatings of different compositions.
FIG. 4 is a graph of the experimental evaluation of the safety of L929 fibroblasts of different component coatings.
FIG. 5 is a graph of experimental evaluation of safety of HUVEC umbilical vein endothelial cells coated with different components.
FIG. 6 is a graph comparing the effect of different component coatings against Staphylococcus aureus.
FIG. 7 is a graph comparing the anti-E.coli effect of different composition coatings.
FIG. 8 is a graph comparing the effect of different component coatings against Pseudomonas aeruginosa.
FIG. 9 is a composition of Ag coating + The release results of (c) are compared with the graph.
Detailed Description
The technical solution of the present invention is further described below by way of specific embodiments.
Unless otherwise specified, various starting materials of the present invention are commercially available or prepared according to conventional methods in the art.
Example one
This example illustrates the preparation of the medical implant coating according to the present invention using artificial hair fibers as a substrate in further detail.
Step one, preparing polyphenol solution and metal salt solution and depositing a metal-polyphenol coating on the surface of artificial hair fiber:
1. firstly, washing artificial hair fibers by using ethanol and deionized water and drying; dissolving tannic acid powder 3.75g in Tris-NO 250ml 3 Preparing a buffer solution (pH = 8.5) into a tannic acid solution, and dissolving 0.004g of silver nitrate powder in 200ml of T in a dark placeris-NO 3 Preparing a silver nitrate solution from the buffer solution;
2. soaking the pretreated artificial hair fiber in a tannic acid solution for 12h sufficiently and uniformly to enable tannic acid to be stably deposited on the surface of the artificial hair fiber to form a precursor coating with reactivity, taking out the artificial hair fiber with the tannic acid deposited on the surface after soaking for 12h, and rinsing for 3 times by using a buffer solution. The artificial hair was then soaked in silver nitrate solution for 10min to form a first tannin-silver nanoparticle coating. And then respectively carrying out cyclic deposition in a tannic acid solution and a silver nitrate solution, soaking each solution for 10min, taking the tannic acid solution and the silver nitrate solution as a cycle every time, and finally carrying out layer-by-layer self-assembly to form 5 layers of tannic acid-silver nanoparticle functional coatings. After rinsing the finally obtained coating three times with a buffer solution, vacuum drying is carried out for standby.
Step two, synthesis of malic acid-1, 8 octanediol polymer:
firstly, weighing 35.5g of L-malic acid powder and 23.6g of 1,8 octanediol powder, adding into a round bottom flask, heating and stirring at 140 ℃ by using an oil bath to dissolve the two; in the reaction process, an organic solvent dioxane can be properly added for proper dissolution to ensure that the reaction is more complete; after the malic acid and the 1, 8-octanediol are completely dissolved, adjusting the temperature to 120 ℃, fully reacting the mixture, and adjusting the stirrer to a reasonable rotating speed to fully and stably react the malic acid and the 1, 8-octanediol;
then, after the malic acid reacts with 1, 8-octanediol for 20 hours, a proper amount of dioxane can be added to properly dissolve the malic acid prepolymer generated by the reaction; and finally, after the dioxane is completely volatilized, pouring the reaction product into deionized water to remove the short-chain polymer which is not completely reacted, and finally, carrying out vacuum freeze drying on the sample to obtain the malic acid-1, 8 octanediol polymer.
Step three, preparing the tannin-silver nanoparticles-polymalic acid coating:
dissolving the malic acid-1, 8-octanediol prepolymer prepared in the step two by using an ethanol solution to prepare a 10% malic acid prepolymer solution, and quickly soaking the artificial hair fiber modified by the tannic acid-silver nanoparticle coating prepared in the step one in the 10% malic acid prepolymer solution to form a thin malic acid prepolymer coating on the surface; the artificial hair fiber was then further crosslinked by heating at 120 ℃ for 3 days to obtain an artificial hair fiber modified with TAP (TA-Ag-POM) coating, and sterilized, dried and stored.
Example two
The preparation method of the medical implant coating layer according to the present invention is described in detail as far as the details are concerned.
Step one, preparing polyphenol solution and metal salt solution and depositing a metal-polyphenol coating on the surface of the polyamide sheet.
1. Firstly, washing a polyamide sheet by using ethanol and deionized water and drying; dissolving 1.5g tannic acid powder in 250ml Tris-NO 3 Preparing a buffer solution (pH = 8.5) into a tannic acid solution, and dissolving 0.006g of silver nitrate powder in 200ml of Tris-NO in the dark 3 Preparing a silver nitrate solution from the buffer solution;
2. and (2) fully and uniformly soaking the pretreated polyamide sheet in a tannic acid solution for 12 hours to ensure that tannic acid can be stably deposited on the surface of the polyamide sheet to form a reactive precursor coating, taking out the polyamide sheet with the tannic acid deposited on the surface after soaking for 12 hours, and rinsing for 3 times by using a buffer solution. The polyamide flakes were then soaked in a silver nitrate solution for 10min to form a first layer of tannic acid-silver nanoparticle coating. And then respectively carrying out cyclic deposition in a tannic acid solution and a silver nitrate solution, soaking each solution for 10min, taking the tannic acid solution and the silver nitrate solution as a cycle each time, and finally, carrying out layer-by-layer self-assembly to form 5 layers of tannic acid-silver nanoparticle functional coatings. After rinsing the finally obtained coating three times with a buffer solution, vacuum drying is carried out for standby.
Step two, synthesis of malic acid polymer:
firstly, 47.4g of L-malic acid powder and 31.6g of 1,8 octanediol powder are weighed and added into a round-bottom flask, and the two are heated, stirred and dissolved at a temperature of 140 ℃ by using an oil bath kettle; in the reaction process, an organic solvent dioxane can be properly added for proper dissolution to ensure that the reaction is more complete; after the malic acid and the 1, 8-octanediol are completely dissolved, adjusting the temperature to 120 ℃, fully reacting the mixture, and adjusting the stirrer to a reasonable rotating speed to fully and stably react the malic acid and the 1, 8-octanediol;
then, after the malic acid and the 1, 8-octanediol react for 22 hours, namely the reaction is complete, adding a proper amount of dioxane to properly dissolve the malic acid prepolymer generated by the reaction; and finally, after the organic solvent is completely volatilized, pouring the reaction product into deionized water to remove the short-chain polymer which is not completely reacted, and finally, carrying out vacuum freeze drying on the sample to obtain the malic acid-1, 8 octanediol polymer (malic acid polymer).
And step three, preparing the tannin-silver nanoparticles-malic acid polymer antibacterial and anti-inflammatory coating.
Dissolving the malic acid-1, 8-octanediol polymer prepared in the step two by using an ethanol solution to prepare a 10% precursor prepolymer solution, and quickly soaking the polyamide sheet modified by the tannic acid-silver nanoparticle coating prepared in the step one in the 10% prepolymer solution to form a thin prepolymer coating on the surface; the polyamide sheet was then further crosslinked by heating at 120 ℃ for 3 days to obtain a polyamide sheet modified with a TAP coating.
SEM detection
SEM tests were performed on the sample prepared in example one and the sample prepared in example two, and the results are shown in fig. 1 and 2. As can be seen from fig. 1 and 2: the surface appearance of the coating is uniform, and the silver nanoparticles are well wrapped in the polymer coating.
Water contact Angle test
Water contact angle test method: dropping a drop of liquid on the surface of a solid (control, TA, (TA-Ag) 3, (TA-Ag) 5 and TAP), photographing an equilibrium system after a solid-liquid-gas three-phase interface is balanced, measuring a contact angle by surface Meter software, and referring to a test result in FIG. 3. In fig. 3, control refers to a polyamide sheet-blank control, TA refers to a layer of TA deposited on the surface of the polyamide sheet, TA-Ag refers to a layer of (TA-Ag) deposited on the surface of the polyamide sheet, TA-Ag 3 refers to a layer of three (TA-Ag) deposited on the surface of the polyamide sheet, TA-Ag 5 refers to a layer of five (TA-Ag) deposited on the surface of the polyamide sheet, and TAP refers to TAP deposited on the surface of the polyamide sheet (fig. 4-8, supra).
As can be seen in fig. 3: the TAP coating has a water contact angle of 56.6 ° ± 1.6 °, indicating that suitable hydrophilicity can render the implant surface more conducive to cell adhesion to the surrounding tissue.
Cell experiments
The experimental method comprises the following steps: the CCK-8 method is adopted to detect the killing effect of the coating on Human Umbilical Vein Endothelial Cells (HUVEC) and L929 fibroblasts. HUVEC and L929 cells were seeded in 24-well plates (5X 104 cells/well), coating modified polyamide sheets (diameter 1.5 cm) were added, and samples (control, TA, (TA-Ag) 3, (TA-Ag) 5, TAP) were incubated with the cells for 24 hours and 72 hours, respectively. After incubation, samples were carefully removed from the 24-well plates with forceps and CCK-8 absorbance measurements were performed according to the protocol of the kit. The solution treated with the CCK-8 kit was transferred to a 96-well plate using a polyamide sheet without a coating layer co-cultured with cells as a control group. The absorbance of the final solution was measured using a multifunctional microplate reader.
The test results are shown in fig. 4 and 5. As can be seen from the figure: the cell survival rates of the L929 fibroblast and HUVEC umbilical vein endothelial cells and the TAP coating group are not statistically different from those of the control group when the cells and the TAP coating group are co-cultured, which indicates that the TAP coating has good biocompatibility.
Bacterial agar plate experiment
Staphylococcus aureus (ATCC 6538), escherichia coli (ATCC 8739) and Pseudomonas aeruginosa (ATCC 15442) were subjected to antibacterial tests. Tryptone Soy Broth Medium (TSB) (Solarbio, china) and Luria-Bertani Medium (LB) (Solarbio, china) were sterilized, and 3 strains were cultured in a bacterial medium at 37 ℃ and cultured overnight with shaking in a constant temperature shaking incubator at 150 rpm. After incubating the bacterial suspension with polyamide membrane samples (control, TA, (TA-Ag) 3, (TA-Ag) 5, TAP) modified with different coatings for 24 hours, a small amount of the bacterial suspension was aspirated and diluted with PBS to the appropriate concentration, and 50. Mu.L of the diluted solution was spread evenly on agar plates (TSA agar plates for Staphylococcus aureus and Escherichia coli; LB agar plates for Pseudomonas aeruginosa). Next, the agar plates were placed in an incubator at 37 ℃ for 18-20 hours, and the number of bacterial colonies on the plates was observed after the colonies grew out. The experimental results refer to fig. 6-8.
As can be seen from FIGS. 6-8, the TAP coating has obvious inhibiting effect on Staphylococcus aureus, escherichia coli and Pseudomonas aeruginosa.
Inductively coupled plasma emission spectroscopy experiment
In order to study Ag in different composition coatings at different time points + The polyamide membrane samples (TA, (TA-Ag) 3, (TA-Ag) 5, TAP each 0.05 g) modified with coatings of different compositions were soaked in 100ml phosphate buffered saline (PBS; pH 7.4). 1ml of supernatant was collected on different days (1, 3, 5, 7, 14, 21, 28, 35, 42, 49, 56, 61 days) and the supernatant was analyzed for Ag by inductively coupled plasma emission spectrometry (ICP-OES; PE Optima 8300, perkinelmer, USA) + The results are shown in FIG. 9.
FIG. 9 shows Ag for TAP group + The release time is close to 60 days and is far higher than 27 days of other groups, which shows that in the group coated by TAP, silver ions are sufficiently and slowly released, and the antibacterial time limit of the implant is effectively prolonged.
The above examples are only intended to illustrate the detailed process of the present invention, and the present invention is not limited to the above detailed process, that is, it is not intended that the present invention necessarily depends on the above detailed process to be carried out. It is understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention and the addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (10)
1. A method for preparing a medical implant coating, comprising the steps of:
s10, preparing a buffer solution, and dissolving tannic acid and silver nitrate in the buffer solution respectively to obtain a tannic acid solution and a silver nitrate solution;
s20, respectively and sequentially circularly depositing the medical implant substrate in a tannic acid solution and a silver nitrate solution to and fro, and forming a plurality of tannic acid-silver nanoparticle coatings on the surface of the medical implant substrate;
s30, soaking the medical implant substrate with the plurality of layers of tannic acid-silver nanoparticle coatings formed on the surface in a malic acid-1, 8-octanediol polymer solution, taking out the medical implant substrate, performing a thermal crosslinking polymerization reaction, and preparing the tannic acid-silver nanoparticle-malic acid polymer coating, namely the medical implant coating, on the surface of the medical implant substrate.
2. The method for preparing a medical implant coating according to claim 1, wherein the concentration of the tannic acid solution is 1-20mg/ml and the concentration of the silver nitrate solution is 0.01-0.2mg/ml in step S10.
3. The method for preparing a medical implant coating according to claim 1, wherein step S20 specifically comprises the steps of:
s20a, soaking the medical implant substrate in a tannic acid solution, uniformly stirring for 12 hours, and depositing a first layer of tannic acid on the surface of the medical implant substrate;
s20b, taking out the medical implant substrate, rinsing the medical implant substrate by adopting a buffer solution, and then soaking the medical implant substrate in a silver nitrate solution under a light-proof condition to obtain a basic tannin-silver nanoparticle coating;
and S20c, repeating the steps S20a and S20b, circularly soaking for 5 cycles, and preparing the tannin-silver nanoparticles in a layer-by-layer self-assembly mode on the surface of the medical implant substrate.
4. The method for preparing a medical implant coating according to claim 3, wherein, from the second soaking, the medical implant substrate is soaked in the tannic acid solution for 10min each time; the soaking time of the medical implant substrate in the silver nitrate solution is 10min each time.
5. The method for preparing a medical implant coating according to claim 1, wherein the method for preparing a malic acid-1, 8 octanediol polymer solution in step S30 comprises the steps of:
adding L-malic acid powder and 1, 8-octanediol powder into a reaction vessel in a mass ratio of 1;
after the malic acid and the 1, 8-octanediol are completely dissolved, reducing the temperature to 120 ℃ and uniformly stirring to perform esterification reaction on the malic acid and the 1, 8-octanediol;
after the esterification reaction of malic acid and 1, 8-octanediol is carried out for 18-24h, adding a solvent to dissolve a reaction product;
after the solvent is completely volatilized, cleaning and vacuum-drying the reaction product to obtain malic acid-1, 8 octanediol polymer;
the malic acid-1, 8 octanediol polymer was dissolved in 6-fluoroisopropanol to prepare a malic acid-1, 8 octanediol polymer solution.
6. The method for preparing a medical implant coating according to claim 5, wherein the mass ratio of 1, 8-octanediol malate polymer to 6-fluoroisopropanol is 1.
7. The method for preparing a medical implant coating according to claim 5,
drying the medical implant substrate with a plurality of layers of tannin-silver nanoparticle coatings formed on the surface soaked in the malic acid-1, 8 octanediol polymer solution;
drying, placing in a vacuum drying oven, heating at 120 deg.C for crosslinking polymerization reaction for 3-5 days, and making into tannin-silver nanoparticle-malic acid polymer coating on the surface of medical implant substrate, i.e. medical implant coating.
8. The method for preparing a medical implant coating according to claim 7, wherein the medical implant substrate having the plurality of tannin-silver nanoparticle coatings formed on the surface thereof is soaked in the malic acid-1, 8 octanediol polymer solution for 1 to 2 seconds.
9. A medical implant coating, characterized in that it is obtained by the production method according to any one of claims 1 to 8.
10. A medical implant, which is characterized by comprising a medical implant substrate and a tannin-silver nanoparticles-malic acid polymer coating deposited on the surface of the medical implant substrate.
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