CN106620896A - Preparation method of chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating - Google Patents

Preparation method of chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating Download PDF

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CN106620896A
CN106620896A CN201710021243.7A CN201710021243A CN106620896A CN 106620896 A CN106620896 A CN 106620896A CN 201710021243 A CN201710021243 A CN 201710021243A CN 106620896 A CN106620896 A CN 106620896A
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chitosan
coating
multilayer film
silver
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CN106620896B (en
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王佰亮
南开辉
徐青文
陈浩
刘慧华
叶子
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Wenzhou Medical University
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/08—Materials for coatings
    • A61L31/082—Inorganic materials
    • A61L31/088—Other specific inorganic materials not covered by A61L31/084 or A61L31/086
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/12—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
    • A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L31/16—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
    • A61L2300/104—Silver, e.g. silver sulfadiazine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/44—Radioisotopes, radionuclides

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Abstract

The invention relates to a preparation method of a chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating. The coating is prepared by using a solution of polyacrylic acid and chitosan-silver nitrate and a layer-by-layer self-assembly method; the heat-reducing nano-silver organic-inorganic hybrid hydrogel coating is obtained by using a heating way. By coating the surface of a multilayer film with a layer of chitosan, the release rate of nano-silver is controlled, and the biocompatibility of the multilayer film is improved. Silver ions have a broad-spectrum antibacterial function, so that the slow release of the silver ions enables the multilayer film to have an efficient and long-acting antibacterial ability for gram-positive and gram-negative bacteria; By coating the surface with the layer of chitosan, the cytotoxicity for fibroblasts and human lens epithelial cells can be reduced, and the biocompatibility is improved. The method is simple and fast in technology and easy to smear, dip and spray, can be implemented in an industrial way, and has wide application scope; the chitosan-coated heat-reducing nano-silver multilayer film long-acting antibacterial coating can effectively improve the antibacterial property and biocompatibility of the surface of a medical device.

Description

A kind of preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of Chitosan-coated Method
Technical field
Present invention relates particularly to new material technology field, and in particular to a kind of thermal reduction Nano Silver multilayer of Chitosan-coated The preparation method of film long-effect antimicrobial coating.
Background technology
The application of biomaterial for medical purpose is more and more extensive, and while medical level is improved, we are also had in the face of it The series of problems for bringing, wherein most it is in the urgent need to address be exactly biomaterial infection problem.Can be by biomaterial sense The process of dye be briefly described for：Microorganism is adsorbed in biomaterial surface, and subsequently field planting forms bacterium colony, and the life of densification is formed then Thing film, discharges swim thalline and toxin, ultimately results in the generation of infection.Bacterium of the nature more than 95% can be by with top Formula forms biomembrane.It is to die from the related infection of biomaterial to have more than 100,000 people every year in the U.S..Therefore its property is not being destroyed Under conditions of energy, by modifying biological surfaces of medical devices, so as to reach anti-bacterial attachment and biofilm formation It is extremely urgent.Current biologic medical equipment surface modification can be divided into anti-bacterial attachment type and antibacterial type.Although antibacterial type can Effectively to kill bacterium, but be also faced with remaining bacteria " corpse " and may cause subinfection again, thus research and development are a kind of both can be with The surface modification method that effective sterilization can also in time discharge bacterium " corpse " is particularly significant.
The characteristics of layer-by-layer has simple, easily operated, and make film layer that there is the complex function of its component, It is expected to obtain having performance that is sterilized and discharging dead bacterium in time concurrently.
The content of the invention
In order to solve the defect and deficiency of prior art.The invention provides a kind of thermal reduction Nano Silver of Chitosan-coated The preparation method of multilayer film long acting antibiotic coating.
The present invention adopt technical solution be：A kind of thermal reduction nanometer Ag multilayer film long acting antibiotic of Chitosan-coated The preparation method of coating, comprises the following steps：
（1）The surface preparation of base material：Clean base material is placed in into the polyethyleneimine of 5mg/ml（PEI）In the aqueous solution, immersion, Obtain the base material of surface amine groups；
（2）Prepare Chitosan-Silver（CHI-Ag+）Solution；
（3）Using polyacrylic acid（PAA）And CHI-Ag+Solution, by the coating process of LBL self-assembly multilayer film is prepared Structure；
（4）The multilayer film for preparing is heated into 0.5-2h, thermal reduction silver ion forms Nano Silver；
（5）Last layer 1-5mg/ml chitosan solutions are uniformly smeared in coating surface, whole Chitosan-coated is so far completed The preparation of multilayer coating.
Described base material is the one kind in glass, quartz, silicon chip, stainless steel, polyester film, silica gel.
Described step（2）Middle Chitosan-Silver（CHI-Ag+）Solution manufacturing method is as follows：Prepare the shell of silver nitrate dissolving Magnetic agitation adjusts pH value in glycan solution, obtains final product Chitosan-Silver（CHI-Ag+）Solution.
Described step（3）The coating process of middle LBL self-assembly is as follows：The base material of surface amine groups is put into 0.5- Soak 6-8 minutes in the polyacrylic acid aqueous solution of 5mg/ml, then clean 2-3 minutes with the washing lotion of same pH, blown with nitrogen It is dry, it is put into the Chitosan-Silver of the 1-5mg/ml containing 0.05-2.0mg/mL silver nitrates（CHI-Ag+）6- is soaked in solution 8min, with the washing lotion of same pH 2-3 minutes are cleaned, and nitrogen is dried up, and so far complete the preparation of a duplicature, repeat to grasp above Make, the preparation until completing whole multilayer coating.
Described step（4）The Nano Silver particle diameter of middle thermal reduction is 5-50nm.
Described step（1）In the base material cleaned be placed in the polyethyleneimine of 5mg/ml（PEI）0.5- is soaked in the aqueous solution 12 hours.
Described step（4）Middle multilayer film heating reduction temperature is 120-150 DEG C.
The invention has the beneficial effects as follows：The invention provides a kind of thermal reduction nanometer Ag multilayer film of Chitosan-coated is long-acting The preparation method of antimicrobial coating, coating of the present invention prepare it is simple, easy, can using dip-coating, spraying etc. can by the way of industrial realization, Applied widely, coating has efficient and long-acting anti-microbial property, can be to the biomedical devices with complex shape structure Carry out coating modification；Coating can improve the biocompatibility of medical device surface, with reduction cytotoxicity；Coating material chemistry Stability Analysis of Structures, endurance, shearing adapt to the interior environment of human body；Coating can realize the ability of the multifunctional antibiotic of wide spectrum.
Specific embodiment
In order to be more clearly understood that the technology contents of the present invention, describe in detail especially exemplified by following examples.
Substrate surface is pre-processed：
By each base material（Glass, quartz, silicon chip, stainless steel, polyester film, silica gel etc.）Jing blades or glass cutter are cut into 1 × 2 cm2 Size, then successively with front Jing ethanol and ultra-pure water, respectively ultrasound 2-3min is cleaned, N2Dry up.The base material for cleaning by clean Base material is placed in and clean base material is placed in the PEI aqueous solution of 1-5mg/ml, soaks 1-12h, obtains the base material of surface amine groups. Base material after base material used is by above-mentioned surface and process in the following example.
Embodiment 1：
The PAA solution of 0.5mg/mL is prepared, it is 4.35 to determine pH value.50ml ultra-pure waters are taken in 50ml centrifuge tubes, adjusting pH is 4.38, it is designated as washing lotion 1.
The silver nitrate for preparing 0.1mg/mL is dissolved in magnetic agitation in the CHI of 1 mg/ml of 50ml, and it is 3.97 to determine pH. It is 4.04 to take 50ml ultra-pure waters and pH value is adjusted in 50ml centrifuge tubes, is designated as washing lotion 2.
Base material after pretreatment is added in PAA solution and soaks 6-8min, taken out, be put into the cleaning 2- of washing lotion 1 3min, takes out, and uses N2Dry up.CHI-Ag is put it into again+6-8min is soaked in solution, is taken out, be put into washing lotion 2 and clean 2- 3min, takes out, and uses N2Dry up, so far complete the preparation of a bilayer, prepare the multilayer coating of 10 bilayers.
After the coating for preparing is heated into 0.5h at 120 DEG C, cutting size is 2x8cm and in surface uniform application The 1ml 5mg/ml CHI aqueous solution, dries in 37 DEG C of baking ovens.
Static contact angle research finds that hydrophily is dramatically increased after film, compares with simple base material, by 67.25 ± 1.43 ° are reduced to 2 ± 0.22 °, under transmission electron microscope observe film layer in Nano Silver distribution, find Nano Silver be evenly distributed on 4.0nm, 9.8nm, 15.6nm, 23.1nm and 23.6nm or so.By antibacterial ring size experiment find antibacterial ring size be respectively 4.3mm, 7.0mm, 15.9mm, 17.1mm and 16.6mm, its antibacterial effect substantially increases.Determine to fibroblast and human lens epithelial cells Cytotoxicity is relatively low, and cell survival rate reaches more than 73%.
Embodiment 2：
The dopa solution of 1mg/mL is prepared, it is 4.36 to determine pH value.50ml ultra-pure waters are taken in 50ml centrifuge tubes, adjusting pH is 4.34, it is designated as washing lotion 1.
The silver nitrate for preparing 0.5mg/mL is dissolved in magnetic agitation in the CHI of 2 mg/ml of 50ml, and it is 4.0 to determine pH. It is 4.20 to take 50ml ultra-pure waters and pH value is adjusted in 50ml centrifuge tubes, is designated as washing lotion 2.
Base material after pretreatment is added in PAA solution and soaks 6-8min, taken out, be put into the cleaning 2- of washing lotion 1 3min, takes out, and uses N2Dry up.CHI-Ag is put it into again+6-8min is soaked in solution, is taken out, be put into washing lotion 2 and clean 2- 3min, takes out, and uses N2Dry up.So far the preparation of a bilayer is completed.It is double-deck that 3,6,9,12,15 are prepared respectively.
After the coating for preparing is heated into 1h at 130 DEG C, cutting size is 2x8cm and in surface uniform application 2ml The 5mg/ml CHI aqueous solution, dries in 37 DEG C of baking ovens.
Using the anti-microbial property of antibacterial ring size experimental evaluation coating, suppression of five kinds of coatings to staphylococcus aureus is as a result measured Collarium is respectively 3.3mm, 9.8mm, 19.9mm, 20.6mm and 23.4mm, and 99.99% concentration can be killed in 15min is 106The Escherichia coli and staphylococcus aureus of CFU/mL.Five kinds of coatings are to fibroblast and human lens epithelial cells poison Property is relatively low, cell survival rate more than 79%, with good biocompatibility.
Embodiment 3：
The dopa solution of 2mg/mL is prepared, it is 3.96 to determine pH value.50ml ultra-pure waters are taken in 50ml centrifuge tubes, adjusting pH is 4.0, it is designated as washing lotion 1.
The silver nitrate for preparing 1mg/mL is dissolved in magnetic agitation in the CHI of the 2.5mg/ml of 50ml, and it is 4.96 to determine pH. It is 4.93 to take 50ml ultra-pure waters and pH value is adjusted in 50ml centrifuge tubes, is designated as washing lotion 2.
Base material after pretreatment is added in PAA solution and soaks 6-8min, taken out, be put into the cleaning 2- of washing lotion 1 3min, takes out, and uses N2Dry up.CHI-Ag is put it into again+6-8min is soaked in solution, is taken out, be put into washing lotion 2 and clean 2- 3min, takes out, and uses N2Dry up.So far the preparation of a bilayer is completed.It is double-deck that 3,6,9,12 are prepared respectively.
After the coating for preparing is heated into 1h at 140 DEG C, cutting size is 2x8cm and in surface uniform application 3ml The 5mg/ml CHI aqueous solution, dries in 37 DEG C of baking ovens.
Measure four kinds of coatings and 7.9mm, 13.2mm, 19.7mm and 31.4mm, equal energy are respectively to colibacillary antibacterial ring size The concentration that 99.99% is killed in 10min is 108The staphylococcus aureus and Escherichia coli of CFU/mL.By bacterium anyway Dyeing is observed and is killed in coating surface, more than 99.9% staphylococcus aureus and Escherichia coli（It is red）, therefore can be with Find out that coating has efficient bactericidal action.Four kinds of coatings are relatively low to fibroblast and human lens epithelial cells toxicity, carefully Cytoactive more than the 78% of TCPS, therefore with good cell compatibility.
Embodiment 4：
With the dopa solution for preparing 3mg/mL, it is 3.87 to determine pH value.50ml ultra-pure waters are taken in 50ml centrifuge tubes, adjusting pH is 3.92, it is designated as washing lotion 1.
The silver nitrate for preparing 2mg/mL is dissolved in magnetic agitation in the CHI of 3 mg/ml of 50ml, and it is 4.09 to determine pH.Take It is 4.12 that 50ml ultra-pure waters adjust pH value in 50ml centrifuge tubes, is designated as washing lotion 2.
Base material after pretreatment is added in PAA solution and soaks 6-8min, taken out, be put into the cleaning 2- of washing lotion 1 3min, takes out, and uses N2Dry up.CHI-Ag is put it into again+6-8min is soaked in solution, is taken out, be put into washing lotion 2 and clean 2- 3min, takes out, and uses N2Dry up.So far the preparation of a bilayer is completed.It is double-deck that 6,9,12,15 are prepared respectively.
After the coating for preparing is heated into 1.5h at 140 DEG C, cutting size is 2x8cm and in surface uniform application The 3.5ml 5mg/ml CHI aqueous solution, dries in 37 DEG C of baking ovens.
Using the distribution of Nano Silver in transmission electron microscope observing coating, as a result show that Nano Silver distribution is more uniform, divide respectively Cloth is in 17.8nm, 25.6nm, 31.9nm and 45.7nm or so.Measure four kinds of coatings to be respectively colibacillary antibacterial ring size 18.7mm, 29.5mm, 40.6mm and 45.2mm, the concentration that 99.99% can be killed in 10min is 106The golden yellow of CFU/mL Staphylococcus and Escherichia coli.By bacterium, anyway dyeing is observed in coating surface, more than 99.9% staphylococcus aureus It is killed with Escherichia coli（It is red）, it can be seen that coating has efficient bactericidal action.Four kinds of coatings are into fiber finer Born of the same parents and human lens epithelial cells toxicity are relatively low, cytoactive more than the 83% of TCPS, therefore with preferable cell compatibility.
Embodiment 5
The dopa solution of 4mg/mL is prepared, it is 5.01 to determine pH value.50ml ultra-pure waters are taken in 50ml centrifuge tubes, adjusting pH is 5.12, it is designated as washing lotion 1.
The silver nitrate for preparing 2mg/mL is dissolved in magnetic agitation in the PEI of 3 mg/ml of 50ml, and it is 4.01 to determine pH.Take It is 4.21 that 50ml ultra-pure waters adjust pH value in 50ml centrifuge tubes, is designated as washing lotion 2.
Base material after pretreatment is added in PAA solution and soaks 6-8min, taken out, be put into the cleaning 2- of washing lotion 1 3min, takes out, and uses N2Dry up.CHI-Ag is put it into again+6-8min is soaked in solution, is taken out, be put into washing lotion 2 and clean 2- 3min, takes out, and uses N2Dry up.So far the preparation of a bilayer is completed.It is double-deck that 6,9,12 are prepared respectively.
After the coating for preparing is heated into 1.5h at 150 DEG C, cutting size is 2x8cm and in surface uniform application The 3.5ml 5mg/ml CHI aqueous solution, dries in 37 DEG C of baking ovens.
Using the distribution of Nano Silver in transmission electron microscope observing coating, as a result show that Nano Silver distribution is more uniform, divide respectively Cloth is in 13.4nm, 20.6nm and 30.5nm or so.Measure three kinds of coatings colibacillary antibacterial ring size is respectively 14.7mm, 29.3mm and 35.7mm, the concentration that 99.9% can be killed in 10min is 107The staphylococcus aureus of CFU/mL and large intestine Bacillus.Dyeed anyway it was observed that being killed in coating surface, more than 99.9% staphylococcus aureus and Escherichia coli by bacterium Extremely（It is red）, it can be seen that coating has efficient bactericidal action.Three kinds of coatings are on fibroblast and people's crystalline lens Chrotoplast toxicity is relatively low, cytoactive more than the 89% of TCPS, therefore with preferable cell compatibility.
Embodiment 6
The dopa solution of 5mg/mL is prepared, it is 4.45 to determine pH value.50ml ultra-pure waters are taken in 50ml centrifuge tubes, adjusting pH is 4.64, it is designated as washing lotion 1.
The silver nitrate for preparing 3mg/mL is dissolved in magnetic agitation in the CHI of the 4mg/ml of 50ml, and it is 5.27 to determine pH.Take It is 5.18 that 50ml ultra-pure waters adjust pH value in 50ml centrifuge tubes, is designated as washing lotion 2.
Base material after pretreatment is added in PAA solution and soaks 6-8min, taken out, be put into the cleaning 2- of washing lotion 1 3min, takes out, and uses N2Dry up.CHI-Ag is put it into again+6-8min is soaked in solution, is taken out, be put into washing lotion 2 and clean 2- 3min, takes out, and uses N2Dry up.So far the preparation of a bilayer is completed.It is double-deck that 6,9,12 are prepared respectively.
After the coating for preparing is heated into 2h at 150 DEG C, cutting size is 2x8cm and in surface uniform application 5ml The 5mg/ml CHI aqueous solution, dries in 37 DEG C of baking ovens.
Static contact angle research finds that hydrophily is dramatically increased after film, compares with simple base material, by 78.35 ± 1.83 ° are reduced to 2 ± 1.62 °.Using the distribution of Nano Silver in transmission electron microscope observing coating, as a result show that Nano Silver distribution is more equal It is even, it is respectively distributed to 12.1nm, 18.7nm and 26.4nm or so.Measure three kinds of coatings to be respectively colibacillary antibacterial ring size 19.8mm, 28.7mm and 39.4mm, the concentration that 99.99% can be killed in 10min is 107 The Staphylococcus aureus of CFU/mL Bacterium and Escherichia coli.Three kinds of coatings are relatively low to fibroblast and human lens epithelial cells toxicity, and cytoactive is more than TCPS 95%, therefore with preferable cell compatibility.
The above is only the preferred embodiment of the present invention, and protection scope of the present invention is not limited merely to above-mentioned enforcement Example, all technical schemes belonged under thinking of the present invention belong to protection scope of the present invention.It should be pointed out that for the art Those of ordinary skill for, some improvements and modifications without departing from the principles of the present invention, these improvements and modifications Should be regarded as protection scope of the present invention.
The above is only the preferred embodiment of the present invention, and protection scope of the present invention is not limited merely to above-mentioned enforcement Example, all technical schemes belonged under thinking of the present invention belong to protection scope of the present invention.It should be pointed out that for the art Those of ordinary skill for, some improvements and modifications without departing from the principles of the present invention, these improvements and modifications Should be regarded as protection scope of the present invention.

Claims (7)

1. a kind of preparation method of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of Chitosan-coated, it is characterised in that bag Include following steps：
（1）The surface preparation of base material：Clean base material is placed in into the polyethyleneimine of 5mg/ml（PEI）In the aqueous solution, immersion, Obtain the base material of surface amine groups；
（2）Prepare Chitosan-Silver（CHI-Ag+）Solution；
（3）Using polyacrylic acid（PAA）And CHI-Ag+Solution, by the coating process of LBL self-assembly multilayer film knot is prepared Structure；
（4）The multilayer film for preparing is heated into 0.5-2h, thermal reduction silver ion forms Nano Silver；
（5）Last layer 1-5mg/ml chitosan solutions are uniformly smeared in coating surface, whole Chitosan-coated is so far completed The preparation of multilayer coating.
2. the preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of a kind of Chitosan-coated according to claim 1 Method, it is characterised in that described base material is the one kind in glass, quartz, silicon chip, stainless steel, polyester film, silica gel.
3. the preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of a kind of Chitosan-coated according to claim 1 Method, it is characterised in that described step（2）Middle Chitosan-Silver（CHI-Ag+）Solution manufacturing method is as follows：Prepare silver nitrate Magnetic agitation adjusts pH value in the chitosan solution of dissolving, obtains final product Chitosan-Silver（CHI-Ag+）Solution.
4. the preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of a kind of Chitosan-coated according to claim 1 Method, it is characterised in that described step（3）The coating process of middle LBL self-assembly is as follows：The base material of surface amine groups is put Enter immersion 6-8 minutes in the polyacrylic acid aqueous solution of 0.5-5mg/ml, then cleaned 2-3 minutes with the washing lotion of same pH, use Nitrogen is dried up, and is put into the Chitosan-Silver of the 1-5mg/ml containing 0.05-2.0mg/mL silver nitrates（CHI-Ag+）Soak in solution 6-8min, with the washing lotion of same pH 2-3 minutes are cleaned, and nitrogen is dried up, and so far complete the preparation of a duplicature, more than repetition Operation, the preparation until completing whole multilayer coating.
5. the preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of a kind of Chitosan-coated according to claim 1 Method, it is characterised in that described step（4）The Nano Silver particle diameter of middle thermal reduction is 5-50nm.
6. the preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of a kind of Chitosan-coated according to claim 1 Method, it is characterised in that described step（1）In the base material cleaned be placed in the polyethyleneimine of 5mg/ml（PEI）In the aqueous solution Immersion 0.5-12 hours.
7. the preparation of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of a kind of Chitosan-coated according to claim 1 Method, it is characterised in that described step（4）Middle multilayer film heating reduction temperature is 120-150 DEG C.
CN201710021243.7A 2017-01-12 2017-01-12 A kind of preparation method of the thermal reduction nanometer Ag multilayer film long acting antibiotic coating of Chitosan-coated Active CN106620896B (en)

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CN107321055A (en) * 2017-07-07 2017-11-07 西南交通大学 Filtering material with sterilizing function and preparation method thereof
CN110373106A (en) * 2019-09-04 2019-10-25 临沂市人民医院 A kind of preparation method of Novel medical automatic cleaning coating material
CN110373106B (en) * 2019-09-04 2020-07-24 临沂市人民医院 Preparation method of medical self-cleaning coating material
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