CN115281319A - Flower collagen liquid and preparation method thereof - Google Patents
Flower collagen liquid and preparation method thereof Download PDFInfo
- Publication number
- CN115281319A CN115281319A CN202210819594.3A CN202210819594A CN115281319A CN 115281319 A CN115281319 A CN 115281319A CN 202210819594 A CN202210819594 A CN 202210819594A CN 115281319 A CN115281319 A CN 115281319A
- Authority
- CN
- China
- Prior art keywords
- emulsion
- sericin
- collagen
- floral
- flower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 43
- 102000008186 Collagen Human genes 0.000 title claims abstract description 37
- 108010035532 Collagen Proteins 0.000 title claims abstract description 37
- 229920001436 collagen Polymers 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000000839 emulsion Substances 0.000 claims abstract description 74
- 108010013296 Sericins Proteins 0.000 claims abstract description 51
- 239000002245 particle Substances 0.000 claims abstract description 42
- 239000000243 solution Substances 0.000 claims abstract description 31
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 19
- 238000003756 stirring Methods 0.000 claims abstract description 17
- 239000011550 stock solution Substances 0.000 claims abstract description 17
- 238000010008 shearing Methods 0.000 claims abstract description 14
- 239000000706 filtrate Substances 0.000 claims abstract description 10
- 239000003292 glue Substances 0.000 claims abstract description 6
- 238000005360 mashing Methods 0.000 claims abstract description 6
- 238000005520 cutting process Methods 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 239000006228 supernatant Substances 0.000 claims abstract description 5
- 235000020939 nutritional additive Nutrition 0.000 claims description 13
- 230000001965 increasing effect Effects 0.000 claims description 9
- 235000013343 vitamin Nutrition 0.000 claims description 8
- 239000011782 vitamin Substances 0.000 claims description 8
- 229940088594 vitamin Drugs 0.000 claims description 8
- 229930003231 vitamin Natural products 0.000 claims description 8
- 239000006172 buffering agent Substances 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000004166 Lanolin Substances 0.000 claims description 5
- 235000019388 lanolin Nutrition 0.000 claims description 5
- 229940039717 lanolin Drugs 0.000 claims description 5
- 239000006041 probiotic Substances 0.000 claims description 5
- 235000018291 probiotics Nutrition 0.000 claims description 5
- 241000220317 Rosa Species 0.000 claims description 4
- 235000015203 fruit juice Nutrition 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 235000013871 bee wax Nutrition 0.000 claims description 3
- 239000012166 beeswax Substances 0.000 claims description 3
- 229920002674 hyaluronan Polymers 0.000 claims description 3
- 229960003160 hyaluronic acid Drugs 0.000 claims description 3
- 239000000787 lecithin Substances 0.000 claims description 3
- 235000010445 lecithin Nutrition 0.000 claims description 3
- 229940067606 lecithin Drugs 0.000 claims description 3
- 239000012527 feed solution Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 12
- 239000000126 substance Substances 0.000 abstract description 5
- 230000003834 intracellular effect Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000004530 micro-emulsion Substances 0.000 abstract description 2
- 238000000265 homogenisation Methods 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 9
- 239000000796 flavoring agent Substances 0.000 description 5
- 235000019634 flavors Nutrition 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 244000215068 Acacia senegal Species 0.000 description 3
- 229920000084 Gum arabic Polymers 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 239000000205 acacia gum Substances 0.000 description 3
- 235000010489 acacia gum Nutrition 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 230000000529 probiotic effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- -1 propylene glycol fatty acid ester Chemical class 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- YVNQAIFQFWTPLQ-UHFFFAOYSA-O [4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylidene]-3-methylcyclohexa-2,5-dien-1-ylidene]-ethyl-[(3-sulfophenyl)methyl]azanium Chemical compound C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=C1 YVNQAIFQFWTPLQ-UHFFFAOYSA-O 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000004833 fish glue Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229940080352 sodium stearoyl lactylate Drugs 0.000 description 1
- ODFAPIRLUPAQCQ-UHFFFAOYSA-M sodium stearoyl lactylate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC(=O)OC(C)C(=O)OC(C)C([O-])=O ODFAPIRLUPAQCQ-UHFFFAOYSA-M 0.000 description 1
- 239000008347 soybean phospholipid Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/20—Fish extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/34—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using microwaves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a floral collagen liquid and a preparation method thereof, wherein the preparation method comprises the following steps: providing the flower glue; cutting the sericin, adding distilled water, and mashing the sericin tissue to obtain a feed liquid; and (3) treating the feed liquid by using microwaves, and filtering the feed liquid after the microwave extraction is finished to obtain filtrate. The microwave extraction ensures that the intracellular pressure exceeds the pressure capacity of the cells to break, and the intracellular substances of the sericin flow out, thereby having the advantage of high-efficiency extraction. Centrifuging the filtrate, and collecting supernatant to obtain sericin extractive solution; adding an emulsifier into the sericin extracting solution under the stirring state, and stirring and shearing to form a first emulsion with large emulsion particles; the first emulsion is homogenized by at least one microjet under the pressurization state to prepare a second emulsion with smaller emulsion particles, namely the flower collagen stock solution. The emulsion with the particle size of only 150nm-180nm is prepared by the method, and the floral collagen stock solution of the microemulsion is more beneficial to absorption by a human body.
Description
Technical Field
The invention relates to the technical field of a floral gel product, in particular to a floral collagen liquid and a preparation method thereof.
Background
The fish glue is made from fish maw by cutting and drying in the sun. The main components of the flower glue are collagen, a plurality of vitamins and a plurality of trace elements such as calcium, zinc, iron, selenium and the like. Based on the nutritional value of the sericin, the sericin is generally used as a cooking food material and is also useful for making drinks, stock solutions, nutrient solutions and the like.
The preparation method of the flower collagen stock solution in the prior art generally needs high-temperature boiling, the high-temperature boiling can cause nutrition loss, and the change of components, flavor and the like due to high temperature can cause the reduction of the nutritional value of the flower collagen stock solution. And the floral gel substance in the floral gel stock solution keeps large gel or dough, which is not easy to absorb.
Disclosure of Invention
The invention mainly aims to provide a preparation method of a flower collagen liquid, and aims to solve the technical problems that components, flavor and the like are changed due to high temperature and are not easy to absorb because the flower collagen liquid in the prior art is decocted at high temperature.
In order to realize the purpose, the preparation method of the flower collagen stock solution provided by the invention comprises the following steps:
providing the flower glue;
adding distilled water after the sericin is cut up, and mashing the sericin tissue to prepare feed liquid;
treating the feed liquid by using microwaves, and filtering the feed liquid after the microwave extraction is finished to obtain filtrate;
centrifuging the filtrate, and taking supernatant to obtain a sericin extracting solution;
adding an emulsifier into the sericin extracting solution under the stirring state, and stirring and shearing to form a first emulsion with larger emulsion particles;
and the first emulsion is homogenized by at least one micro-jet under the pressurization state to prepare a second emulsion with smaller emulsion particles, namely the flower collagen stock solution.
Optionally, in the step of adding distilled water after the sericin is chopped, the mass ratio of the sericin to the distilled water is 1: (40-50).
Optionally, in the step of treating the feed liquid with microwaves, the microwave extraction time is 3 minutes to 5 minutes, and the microwave extraction power is 350W to 400W.
Optionally, in the step of stirring and shearing to form the first emulsion with larger emulsion particles, the shearing speed is 12000r/min-14000r/min, and the shearing time is 3 minutes-4 minutes.
Optionally, in the step of preparing the second emulsion with smaller emulsion particles by at least one micro-jet homogenization of the first emulsion under the pressurized state, the pressure is increased to 130MPa-140MPa.
Optionally, in the step of preparing a second emulsion with smaller emulsion particles by at least one time of microfluidization homogenization of the first emulsion under a pressurized state, the microfluidization is performed by using a high-pressure microfluidization nano-homogenizer for 4-7 times of microfluidization.
Optionally, a nutritional additive is added in the step of adding the emulsifier into the flower gum extracting solution under stirring, wherein the addition amount of the nutritional additive is 1-20% of the mass of the flower gum extracting solution, and the nutritional additive is at least one of fruit juice, hyaluronic acid, probiotics, rose juice or vitamins.
Optionally, the emulsifier is selected from at least one of lecithin, lanolin alcohol, lanolin acid, or beeswax; the addition amount of the emulsifier is 3-11% of the mass of the sericin extracting solution.
Optionally, before the step of treating the feed liquid with microwaves, the method further comprises the following steps: and adding a buffering agent into the feed liquid, wherein the addition amount of the buffering agent is 10-23% of the feed liquid.
The invention also provides a floral collagen solution which is prepared by the preparation method of the floral collagen solution.
According to the technical scheme, the sericin is pretreated through microwave extraction, the microwave extraction is favorable for keeping the nutritional ingredients and the flavor of the sericin, cells in the sericin can absorb microwave energy to rapidly and integrally heat up, the pressure in the cells exceeds the pressure bearing capacity of the cells to break, the substances in the cells of the sericin flow out, and the extraction method has the advantage of high-efficiency extraction. The sericin extract obtained after microwave extraction is stirred and sheared to form a first emulsion with larger emulsion particles, a second emulsion with smaller emulsion particles is prepared through at least one-time microfluidization, the emulsion with the particle size of only 150nm-180nm is prepared through the method, and the sericin stock solution of the microemulsion is more beneficial to absorption by a human body.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a preparation method of a floral collagen liquid.
In the embodiment of the invention, the preparation method of the flower collagen stock solution comprises the following steps:
providing the flower glue;
adding distilled water after cutting the sericin, and mashing the sericin tissue to obtain a feed liquid; this step can be achieved by means of a tissue triturator;
treating the feed liquid by using microwaves, and filtering the feed liquid after the microwave extraction is finished to obtain filtrate; this step may be achieved by a microwave oven;
centrifuging the filtrate, and collecting supernatant to obtain a sericin extract; this step may be achieved by a centrifuge;
adding an emulsifier into the sericin extracting solution under the stirring state, and stirring and shearing to form a first emulsion with large emulsion particles; this step can be achieved by a high speed emulsification shear apparatus;
the first emulsion is homogenized by at least one microjet under the pressurization state to prepare a second emulsion with smaller emulsion particles, namely the flower collagen stock solution.
The technological scheme of the present invention includes microwave extraction to pretreat the flower gum and to maintain the nutritious components and flavor of the flower gum, and the microwave extraction makes the cells in the flower gum absorb microwave energy to raise temperature fast and integrally, so that the intracellular pressure exceeds its pressure bearing capacity to break and the intracellular matter of the flower gum flows out. The sericin extracting solution obtained after microwave extraction is stirred and sheared to form a first emulsion with larger emulsion particles, a second emulsion with smaller emulsion particles is prepared through at least one-time microfluidization homogenization, and the emulsion with the particle size of only 150nm-180nm is prepared through the method.
In the embodiment of the invention, in the step of adding distilled water after the sericin is cut up, the mass ratio of the sericin to the distilled water is 1: (40-50). Specifically, the mass ratio is 1: 40. 1:46 or 1:50, the mass ratio may also be 1: (40-50).
In the embodiment of the invention, in the step of treating the feed liquid by microwaves, the microwave extraction time is 3 minutes to 5 minutes, and the microwave extraction power is 350W to 400W. Specifically, the microwave extraction time may be 3 minutes, 4 minutes or 5 minutes, or any time in the interval of 3 to 5 minutes. The microwave extraction power can be 350W, 370W or 400W, or any power in the interval of 350W-400W.
In the embodiment of the invention, in the first emulsion step of stirring and shearing to form larger emulsion particles, the shearing speed is 12000r/min-14000r/min, and the shearing time is 3 minutes-4 minutes. Specifically, the shearing speed can be 12000r/min, 13000r/min or 14000r/min, and can also be any stirring speed in the interval of 12000r/min-14000 r/min.
In the embodiment of the invention, in the second emulsion step of preparing the first emulsion with smaller emulsion particles by at least one micro-jet homogenization under the pressurized state, the pressure is increased to 130MPa-140MPa. Specifically, the pressurization pressure may be 130MPa, 135MPa or 140MPa, and may be any pressure value in the range of 130MPa to 140MPa.
In the embodiment of the invention, in the step of preparing the second emulsion with smaller emulsion particles by at least one time of micro-jet homogenization of the first emulsion under the pressurization state, a high-pressure micro-jet nano homogenizer is adopted for micro-jet homogenization, and the micro-jet homogenization frequency is 4-7 times. Specifically, the number of homogenization may be 4, 5, 6, or 7.
In the embodiment of the invention, a nutritional additive is also added in the step of adding the emulsifier into the flower gum extracting solution under the stirring state, the addition amount of the nutritional additive is 1-20% of the mass of the flower gum extracting solution, and the nutritional additive is selected from at least one of fruit juice, hyaluronic acid, probiotics, rose juice or vitamins. The nutritional additive is mainly used for enhancing the nutritional effect of the sericin, and the addition amount of the nutritional additive can be 1%, 5%, 10% or 20%, or any one of 1% to 20%.
The flower collagen stock solution can be used as a novel product such as an effective beverage after being added with the nutritional additive.
Illustratively, the probiotic is added, so that the probiotic is easy to absorb, the damaged intestinal mucosa is repaired, and the intestinal flora is adjusted. The vitamins can be supplemented by adding vitamins. The vitamin of the invention is specifically at least one of the vitamins A-E. The fruit juice and the raw materials in the category of homology of medicine and food are added, so that the effects of enriching nutrient components, increasing flavor and the like are achieved. The rose juice can be added to achieve the effect of beautifying.
In the embodiment of the invention, the emulsifier is at least one selected from lecithin, lanolin alcohol, wool acid or beeswax, and is a natural emulsifier which is non-toxic or extremely low in toxicity to human bodies. The addition amount of the emulsifier is 3-11% of the mass of the sericin extracting solution. The emulsifier is used for forming stable emulsion, and other types of emulsifiers can be added according to different application scenes, such as SPAN (SPAN), tween (TWEEN), sodium stearoyl lactylate-calcium, sodium stearoyl lactylate, triglycerin, propylene glycol fatty acid ester, sucrose ester, soybean phospholipid, lauric monoglyceride and the like, namely the emulsifier is not limited to the natural emulsifier and only needs to realize the emulsifying effect.
In the embodiment of the invention, before the step of treating the feed liquid by microwaves, the method further comprises the following steps: adding a buffering agent into the feed liquid, wherein the addition amount of the buffering agent is 10-23% of the feed liquid. The buffer may be phosphate buffer solution with pH =7.4, which is isotonic with human blood and contains sodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride and potassium chloride as main ingredients.
For a further understanding of the invention, reference will now be made to the preferred embodiments of the invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the invention, and not to limit the scope of the claims.
The following preparation method of a sericin stock was adopted in each of examples and comparative examples, and the steps were as follows:
providing the flower glue;
adding distilled water after cutting the sericin, and mashing the sericin tissue by a tissue mashing machine to prepare a feed liquid; adding a buffering agent to the feed liquid;
treating the feed liquid with microwave, and filtering the feed liquid after microwave extraction to obtain filtrate;
centrifuging the filtrate, and collecting supernatant to obtain a sericin extract;
adding an emulsifier and a nutritional additive into the flower gum extracting solution under the stirring state, and stirring and shearing to form a first emulsion with larger emulsion particles;
the first emulsion is homogenized by at least one microjet under the pressurization state to prepare a second emulsion with smaller emulsion particles, namely the flower collagen stock solution.
See table 1 for process parameters used in examples 1-3 and comparative examples 1 and 2. The extraction rate of the gum extract and the emulsion particle size of the gum stock of examples 1-3 and comparative examples 1 and 2 were measured, and the results are shown in Table 1.
The extraction rate test method of the sericin extracting solution comprises the following steps: firstly, the protein content of the sericin is determined by using a Kjeldahl method, and the extraction rate is determined when the soluble protein content of the sericin extracting solution is determined, wherein the soluble protein content accounts for the protein content of the sericin.
Soluble protein content test method:
100mg of Coomassie brilliant blue G-250 was weighed, dissolved in 50ml of 95% ethanol, added with 100ml of 85% phosphoric acid, diluted to 1000ml with distilled water, and filtered through a filter paper to obtain a 0.01% Coomassie brilliant blue sample solution.
Accurately weighing 10mg bovine serum albumin standard substance, adding distilled water to constant volume to 100ml, and obtaining 100ug/ml standard protein solution. Taking 0, 0.2, 0.4, 0.6, 0.8 and 1.0ml of standard protein solution, then adding distilled water to 1ml, adding 5ml of Coomassie brilliant blue test solution respectively, shaking uniformly, standing at room temperature for 3min, measuring absorbance at 595nm respectively, and drawing a standard curve by taking the absorbance as an ordinate and the standard protein content as an abscissa.
Taking 10ml of extracting solution to be put into a 100ml volumetric flask, and adding distilled water to the full scale position for constant volume. And (3) according to the standard working curve making steps, respectively measuring the absorbance at the wavelength of 595nm, and then calculating the protein content of the sample by using the standard curve.
The method for measuring the emulsion particle size of the flower collagen stock solution comprises the following steps:
diluting the sericin stock solution by 500 times with distilled water, testing the particle size of the emulsion by using a laser particle sizer at room temperature, and acquiring data after 120 seconds of balance time. Each sample was subjected to 3 replicates and the final results are expressed as mean particle size.
Placing 100mL of the sericin extract in a 100mL volumetric flask, adding distilled water to the scale position for constant volume,
TABLE 1
As can be seen from table 1, the mass ratio of the gum arabic to the distilled water affects the extraction rate of the substances inside the gum arabic, and the extraction rate increases and then decreases with the decrease of the mass ratio, wherein 1: at 46, the extraction rate reached a maximum. During the subsequent microfluidization, the emulsion particles obtained are also related to the mass ratio, when the mass ratio is 1: and when 55 hours, the average particle size of the second emulsion is the largest, and the analysis shows that the protein concentration in the emulsion is too low, the emulsion can not reach saturation even if the emulsion is completely adsorbed on an oil-water interface, so that small droplets in the solution are agglomerated to form the emulsion with larger particle size. When the mass ratio is 1:46, at which point the emulsion particle size is minimized. When the mass ratio of the sericin to the distilled water continues to increase, the particle size of the emulsion has a slow rising trend, which shows that as the protein is continuously adsorbed, the relative equilibrium state between the droplets is destroyed, and the combination of protein molecules adsorbed on different droplets causes the polymerization between the emulsions so as to increase the particle size.
See table 2 for process parameters used in examples 2, 4, 5 and comparative examples 3, 4. The extraction ratio of the extract liquid of the sericin in examples 4 to 6 and comparative examples 3 and 4 was measured, and the test results are shown in Table 2.
TABLE 2
As can be seen from Table 2, the extraction rate of the gum arabic extract was increased with the increase of the microwave extraction power, wherein the increase was the greatest at 350W, and the change of the extraction rate was gradual when the power exceeded 400W. When analyzing, the larger the microwave power is, the more energy the cells in the feed liquid absorb, the larger the degree of cell disruption is, and the more collagen is dissolved out. However, when the microwave power is too high, the energy absorbed by the feed liquid is too high, the temperature is too high, and the protein is deformed to reduce the solubility of the protein.
See table 3 for process parameters used in examples 2, 5, 6 and comparative examples 5, 6. The emulsion particle size of the floral collagen liquid of examples 2, 5, 6 and comparative examples 5, 6 was determined and the results are shown in table 3.
TABLE 3
As can be seen from Table 3, the average particle diameter gradually decreased as the pressurizing pressure increased, indicating that increasing the pressure within a certain range is advantageous for improving the homogenization efficiency. However, as the pressure increases, the production cost and equipment requirements increase, and therefore, the pressurization pressure is preferably 135MPa.
See table 4 for process parameters used in examples 2, 7, 8 and comparative examples 7, 8. The latex particle size of the floral collagen liquid of examples 2, 7, 8 and comparative examples 7, 8 was determined and the results are shown in table 4.
TABLE 4
Generally, as the number of homogenization increases, the particle size of the latex of the floral collagen liquid also decreases. However, as is clear from Table 4, the particle size of the latex of the floral collagen liquid decreased first and then increased as the number of homogenization treatments increased. The analysis is because the energy provided by the homogenization process causes the aggregation of the droplets, i.e., an over-treatment phenomenon, when the number of homogenization times exceeds 5, and thus, the number of homogenization times is more preferably 5.
The invention also provides a flower collagen solution, which is prepared by the preparation method of the flower collagen solution, the preparation method of the flower collagen solution refers to the above embodiments, and the flower collagen solution adopts all technical schemes of all the embodiments, so that the flower collagen solution at least has all the beneficial effects brought by the technical schemes of the embodiments, and the details are not repeated herein.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all equivalent modifications made by the present specification or directly/indirectly applied to other related technical fields under the inventive concept are included in the scope of the present invention.
Claims (10)
1. The preparation method of the floral collagen liquid is characterized by comprising the following steps of:
providing the flower glue;
cutting the sericin, adding distilled water, and mashing the sericin tissue to obtain a feed liquid;
treating the feed liquid by using microwaves, and filtering the feed liquid after the microwave extraction is finished to obtain filtrate;
centrifuging the filtrate, and taking supernatant to obtain a sericin extracting solution;
adding an emulsifier into the sericin extracting solution under the stirring state, and stirring and shearing to form a first emulsion with larger emulsion particles;
and the first emulsion is homogenized by at least one micro-jet under the pressurization state to prepare a second emulsion with smaller emulsion particles, namely the flower collagen stock solution.
2. The method for preparing a sericin stock according to claim 1, wherein in the step of adding distilled water after the sericin is chopped, the mass ratio of the sericin to the distilled water is 1: (40-50).
3. The method for preparing a sericin stock solution according to claim 1, wherein in the step of treating the feed solution with microwaves, the microwave extraction time is 3 minutes to 5 minutes, and the microwave extraction power is 350W to 400W.
4. The method of claim 1, wherein the first step of stirring and shearing to form a first emulsion with larger emulsion particles comprises shearing at 12000r/min-14000r/min for 3 min-4 min.
5. The method for preparing a flower collagen solution according to claim 1, wherein the pressure is increased to 130MPa to 140MPa in the step of preparing a second emulsion having relatively small emulsion particles by homogenizing the first emulsion by at least one microfluidization under pressure.
6. The method according to claim 1, wherein in the step of preparing the second emulsion having relatively small emulsion particles by at least one microfluidization under pressure of the first emulsion, the microfluidization is performed using a high-pressure microfluidization nanomixer for 4 to 7 microfluidization times.
7. The method for preparing a floral collagen solution as claimed in any one of claims 1 to 6, wherein a nutritional additive is further added in the step of adding the emulsifier to the floral gum extract under stirring, wherein the amount of the nutritional additive added is 1% -20% by mass of the floral gum extract, and the nutritional additive is at least one selected from fruit juice, hyaluronic acid, probiotics, rose juice and vitamins.
8. The method of preparing a floral collagen liquid as claimed in any one of claims 1 to 6, wherein said emulsifier is selected from at least one of lecithin, lanolin alcohol, lanolin acid or beeswax; the addition amount of the emulsifier is 3-11% of the mass of the sericin extracting solution.
9. The method for preparing a sericin stock according to any one of claims 1 to 6, further comprising the following steps before the step of treating the feed with microwaves: and adding a buffering agent into the feed liquid, wherein the addition amount of the buffering agent is 10-23% of the feed liquid.
10. A floral collagen liquid, prepared by the method of any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210819594.3A CN115281319A (en) | 2022-07-12 | 2022-07-12 | Flower collagen liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210819594.3A CN115281319A (en) | 2022-07-12 | 2022-07-12 | Flower collagen liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115281319A true CN115281319A (en) | 2022-11-04 |
Family
ID=83822749
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210819594.3A Pending CN115281319A (en) | 2022-07-12 | 2022-07-12 | Flower collagen liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115281319A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104672323A (en) * | 2013-11-29 | 2015-06-03 | 中国科学院兰州化学物理研究所 | Preparation method of fish collagen |
| CN106819741A (en) * | 2017-01-20 | 2017-06-13 | 世茂（苏州）生物科技有限公司 | Compound fruit-flavored beverage of a kind of Isin glue collagen and preparation method thereof |
| DE102016109094A1 (en) * | 2016-05-18 | 2017-11-23 | Gelita Ag | Process for the preparation of a collagenous material in particulate form and collagen material produced |
| CN107502643A (en) * | 2017-09-29 | 2017-12-22 | 句容市空凤来仪商贸有限公司 | A kind of colored glue polypeptide production methods |
| CN107573415A (en) * | 2017-10-16 | 2018-01-12 | 海南三元星生物科技股份有限公司 | A kind of extracting method of Isin glue collagen and its application |
| CN108936691A (en) * | 2018-05-31 | 2018-12-07 | 张志霞 | Flower glue extracting solution and preparation method thereof, collagen peptide food of beautifying face and moistering lotion comprising the flower glue extracting solution and preparation method thereof |
| CN110692879A (en) * | 2019-10-12 | 2020-01-17 | 浙江英树生物科技有限公司 | Collagen peptide drink and preparation method thereof |
-
2022
- 2022-07-12 CN CN202210819594.3A patent/CN115281319A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104672323A (en) * | 2013-11-29 | 2015-06-03 | 中国科学院兰州化学物理研究所 | Preparation method of fish collagen |
| DE102016109094A1 (en) * | 2016-05-18 | 2017-11-23 | Gelita Ag | Process for the preparation of a collagenous material in particulate form and collagen material produced |
| CN106819741A (en) * | 2017-01-20 | 2017-06-13 | 世茂（苏州）生物科技有限公司 | Compound fruit-flavored beverage of a kind of Isin glue collagen and preparation method thereof |
| CN107502643A (en) * | 2017-09-29 | 2017-12-22 | 句容市空凤来仪商贸有限公司 | A kind of colored glue polypeptide production methods |
| CN107573415A (en) * | 2017-10-16 | 2018-01-12 | 海南三元星生物科技股份有限公司 | A kind of extracting method of Isin glue collagen and its application |
| CN108936691A (en) * | 2018-05-31 | 2018-12-07 | 张志霞 | Flower glue extracting solution and preparation method thereof, collagen peptide food of beautifying face and moistering lotion comprising the flower glue extracting solution and preparation method thereof |
| CN110692879A (en) * | 2019-10-12 | 2020-01-17 | 浙江英树生物科技有限公司 | Collagen peptide drink and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 余祥彬: "《药剂学与药物动力学实验指导》", vol. 1, 厦门大学出版社, pages: 93 * |
| 段振华等: ""微波技术在鱼鳔蛋白提取中的应用"", 《食品科学》, vol. 30, no. 16, pages 149 - 152 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5613953B2 (en) | Use of functional materials containing citrus pulp in Wenzhou | |
| CN109287770B (en) | Solid tea oil rich in polyphenol and preparation method thereof | |
| CN110710577A (en) | Preparation method of high-oil-loading-capacity emulsion rich in fibrous polysaccharide | |
| CN110089694B (en) | Egg yolk-phytosterol-polysaccharide composite emulsion gel and preparation method thereof | |
| Manak et al. | Functioning potential of soy, cottonseed, and peanut protein isolates produced by industrial membrane systems | |
| CN115088830B (en) | Alginate-based microbead simultaneously loaded with multipolar functional factors, and preparation method and application thereof | |
| CN113966830A (en) | Preparation method of novel active substance-loaded vegetable protein-polysaccharide composite emulsion gel | |
| CN113925157B (en) | Beta-carotene emulsion and preparation method thereof | |
| CN108064976A (en) | A kind of method that polysaccharide emulsion gel is prepared using Pickering lotions | |
| CN108113008B (en) | Emulsion for reducing fat digestion and preparation method and application thereof | |
| CN114009691A (en) | China-hemp sour soybean milk and preparation method thereof | |
| CN113974137A (en) | Process for preparing polysaccharide-based fat meat tissue based on microcapsule technology | |
| CN115281319A (en) | Flower collagen liquid and preparation method thereof | |
| CN115226784B (en) | Method for preparing special fat for plant meat based on microalgae protein and application of special fat | |
| CN114600969B (en) | Preparation method of solid lipid based on pig plasma protein | |
| CN114947107B (en) | Preparation and application of modified pea protein-chitosan nanoparticle | |
| KR101773843B1 (en) | Sweet Jelly Containing Cranberry With Antioxidant Activity And Method for Preparing thereof | |
| CN111937969B (en) | Preparation method of sunflower seed oil-based emulsion gel based on beet pectin and beta-cyclodextrin | |
| CN104982538B (en) | A kind of production method of maca Walnut Milk | |
| CN114365841A (en) | Total nutrient milk suitable for patients with nephropathy and preparation method thereof | |
| CN105454438B (en) | Soybean milk powder and preparation method thereof | |
| CN115644261B (en) | Preparation method of fermented bean dreg stable nutritional solid fat | |
| CN113768005B (en) | Macadamia nut tea and preparation method thereof | |
| AU2021100182A4 (en) | A preparation method for micro capsule powder of eucommia ulmoides seed oil and its application | |
| CN115349556B (en) | Peanut oil body-based vegetable meat substitute fat and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |