CN115267227A

CN115267227A - Reagent bottle and kit and reagent storage assembly of using thereof

Info

Publication number: CN115267227A
Application number: CN202210834156.4A
Authority: CN
Inventors: 张帅; 向裕; 柳邦源
Original assignee: Zhuhai Livzon Diagnostics Inc
Current assignee: Zhuhai Livzon Diagnostics Inc
Priority date: 2022-07-14
Filing date: 2022-07-14
Publication date: 2022-11-01

Abstract

The application relates to the technical field of medical equipment, in particular to a reagent bottle, a kit with the reagent bottle and a reagent storage assembly with the reagent bottle. The reagent bottle is characterized in that a bottle body is arranged in a hollow mode, at least two stirring ribs are fixedly arranged in the reagent bottle at intervals, the stirring ribs extend upwards from the bottom of the bottle body along the extension direction of the bottle body, turbulent surfaces with staggered and continuous concave-convex surfaces are arranged on the outer walls of the stirring ribs, each turbulent surface is provided with at least one convex part and at least one concave part, the convex parts and the concave parts are sequentially arranged in a staggered mode along the extension direction of the stirring ribs, and the convex parts or the concave parts of one stirring rib are arranged corresponding to the concave parts or the convex parts of the other stirring rib adjacent to the convex parts or the concave parts at the same height. The reagent bottle is arranged in the reagent box, and the reagent storage assembly comprises the reagent box. This reagent bottle and kit and reagent storage assembly of using thereof can make better being in the turbulent state of reagent, more do benefit to the mixing of reagent, strengthen the homogeneity of reagent mixing.

Description

Reagent bottle and kit and reagent storage assembly of using thereof

Technical Field

The application relates to the technical field of medical equipment, in particular to a reagent bottle, a kit with the reagent bottle and a reagent storage assembly with the reagent bottle.

Background

At present, immunodiagnosis detects and uses automatic analysis system to detect patient's sample more, in the testing process, must carry out the mixing earlier with the detect reagent among the automatic analysis system and detect again, and among the prior art, in order to make things convenient for the preparation of reagent bottle, a stirring muscle that is used for putting the inside of detect reagent's reagent bottle to be equipped with generally forms an organic wholely with the reagent bottle, the shape of stirring muscle is mostly rectangle or trapezoidal, a plurality of stirring muscle also are evenly distributed in the reagent bottle, this kind of structure is discover in the use, there is the not enough problem of reagent homogeneity through stirring muscle mixture in the reagent bottle.

Disclosure of Invention

The application aims to provide a reagent bottle and a kit and a reagent storage assembly applied by the reagent bottle to solve the technical problem that reagent uniformity is insufficient after reagent in the reagent bottle is uniformly mixed.

In a first aspect, the present application provides a reagent bottle,

the reagent bottle is characterized in that the body of the reagent bottle is hollow, at least two stirring ribs are fixedly arranged in the reagent bottle at intervals, the stirring ribs extend upwards from the bottom of the body along the extension direction of the body, turbulent surfaces with staggered and continuous concave-convex surfaces are arranged on the outer walls of the stirring ribs, each turbulent surface is provided with at least one convex part and at least one concave part, the convex parts and the concave parts are sequentially arranged in a staggered mode along the extension direction of the stirring ribs, and the convex part or the concave part of one stirring rib is arranged corresponding to the concave part or the convex part of the other stirring rib adjacent to the convex part on the same height.

Furthermore, at least two stirring ribs are arranged in the bottle body around the central shaft of the bottle body, and the turbulent flow surface of each stirring rib is arranged towards the central shaft.

Furthermore, the stirring rib is fixedly connected with the inner wall of the bottle body in an integrated forming mode or the side wall of the stirring rib is inserted into the inner wall of the bottle body in a fixedly connected mode.

Further, the bottle body is cylindrical;

the extension height of the stirring rib is 50% -70% of the height of the inner cavity of the bottle body;

the thickness of the stirring rib is 30% -50% of the wall thickness of the bottle body;

the width of the stirring rib is 10% -20% of the diameter of the inner cavity of the bottle body.

Furthermore, the extension height of the stirring rib is 60% of the height of the inner cavity of the bottle body;

the thickness of the stirring rib is 40% of the wall thickness of the bottle body;

the width of the stirring rib is 17% of the diameter of the inner cavity of the bottle body.

Furthermore, the turbulent surface is arranged into a sawtooth structure or a wavy structure, the shape of the convex part of the turbulent surface is tooth-shaped, the tooth is angular with a tip, or rectangular or arc-shaped with a gentle surface, and the maximum protruding height of the tooth is less than or equal to 60% of the width of the stirring rib.

Furthermore, the bottle body is cylindrical, the outer wall of the bottle body is provided with a clamping part,

the clamping part is an annular groove arranged in the circumferential direction of the lower portion of the outer wall of the bottle body in a surrounding mode, or the clamping part is at least two bulges arranged in the circumferential direction of the outer wall of the bottle body in a surrounding mode or annular bulges arranged in the circumferential direction of the outer wall of the bottle body in a surrounding mode.

Furthermore, the upper portion of the bottle body extends outwards and is provided with a limiting portion, and the limiting portion is arranged to be a limiting protrusion, or a plurality of protrusions arranged around the limiting protrusion, or an annular protruding outer edge arranged around the limiting protrusion.

In a second aspect, the present application provides a reagent kit, in which any one of the reagent bottles is installed, the reagent kit comprising a housing and a reagent bottle installation site arranged in the housing, wherein the reagent bottle is installed on the reagent bottle installation site, and the reagent bottle installation site is provided with a clamping protrusion or a clamping groove for clamping the reagent bottle;

and a first clamping position and a second clamping position are respectively arranged on two opposite side walls of the outer wall of the shell.

In a third aspect, the application provides a reagent storage assembly, which comprises the reagent kit, a reagent turntable and a reagent turntable rotating disc driving assembly, wherein the reagent turntable is provided with at least one reagent kit mounting groove along the circumferential direction thereof and is used for mounting the reagent kit, the reagent kit mounting groove is provided with a clamping position for clamping the first clamping position and the second clamping position, and the bottom of the reagent kit mounting groove is rotatably connected with a reagent blending mechanism.

Compared with the prior art, the reagent bottle that this application provided and kit and reagent of using store the subassembly, are provided with two at least stirring muscle in this reagent bottle, and the outer wall of stirring muscle has the crisscross continuous turbulent flow face of concave-convex face, and convex part and concave part set up along the direction of stretching out of stirring muscle staggered arrangement in proper order, and on the same height, the relative convex part of concave part of two adjacent stirring muscle, the setting of crisscross arranging of the relative concave part of convex part. Set up like this and to make the reagent bottle when horizontal rotation, the liquid in the reagent bottle is similar heliciform rotation, can be better be in the turbulent state, more do benefit to the mixing of reagent, the homogeneity of reinforcing reagent mixing.

Drawings

In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings needed to be used in the detailed description of the present application or the prior art description will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural diagram of a reagent bottle provided in an embodiment of the present application;

FIG. 2 is a cross-sectional view of a reagent bottle provided in an embodiment of the present application;

FIG. 3 is a sectional view of a reagent bottle mounted in a reagent bottle mounting position in a reagent cartridge according to an embodiment of the present application;

FIG. 4 is a schematic diagram of a kit provided in an embodiment of the present application;

FIG. 5 is a cross-sectional view of a portion of a reagent storage assembly according to an embodiment of the present application;

fig. 6 is a schematic structural diagram of a reagent bottle according to an embodiment of the present disclosure.

Reference numerals:

100-reagent bottle;

10-a bottle body;

11-stirring ribs;

111-a turbulent surface;

111 a-projections;

111 b-a recess;

12-an annular groove;

121-annular upper top surface;

13-a limiting part;

14-ring thread;

15-EP film;

16-cross silica gel membrane;

20-bottle cap;

21-a notch;

200-kit;

201-reagent bottle mounting position;

2011-snap protrusions;

202-a housing;

2021-first card position;

2022-second card position;

300-reagent carousel;

301-kit mounting groove;

3011-bump.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships that the product of the application is conventionally placed in use, and are used only for convenience of describing the present application and simplifying the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it should also be noted that, unless expressly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and can include, for example, fixed connections, detachable connections, or integral connections; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in this application will be understood to be a specific case for those of ordinary skill in the art.

Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

As shown in fig. 1 to 5, the present application provides a reagent bottle 100 and a reagent kit 200 and a reagent storage assembly using the same, the reagent kit 200 includes a housing 202, at least one reagent bottle mounting location 201 is disposed in the housing 202 for mounting at least one reagent bottle 100, in the figure, three reagent bottles 100 are mounted side by side, for example, two opposite side walls of an outer wall of the housing 202 of the reagent kit 200 may further be respectively provided with a first clamping location 2021 and a second clamping location 2022 for clamping with the reagent storage assembly. This reagent storage assembly is used for storing this kit 200, this reagent storage assembly can include reagent carousel 300 and drive this reagent carousel 300 pivoted carousel drive assembly, this reagent carousel 300 is last can be provided with at least one kit mounting groove 301 along its circumference, be used for installing this kit 200, the relative both sides of this kit mounting groove 301 still are equipped with the block position that is used for with aforementioned first joint position 2021 and second joint position 2022 block, still can be equipped with the protruding 3011 with the reagent bottle 100 block in the kit 200 on this kit mounting groove 301, be used for with this reagent bottle 100 block and drive its horizontal rotation mixing, it is corresponding, the bottom surface of the body 10 of this reagent bottle 100 can be equipped with at least one recess, be used for with this protruding 3011 block, the bottom of kit mounting groove 301 still rotates with reagent mixing mechanism and is connected, this reagent mixing mechanism is used for driving reagent bottle 100 horizontal rotation mixing.

As shown in fig. 2 and 3, the reagent bottle 100 provided by the present application may include a bottle body 10 and a bottle cap 20 covering a top opening of the bottle body 10. The bottle body 10 is hollow, at least two stirring ribs 11 are fixedly arranged in the bottle body 10 at intervals, the stirring ribs 11 extend upwards from the bottom of the bottle body 10 along the extension direction of the bottle body 10, turbulent surfaces 111 with continuous concave-convex surfaces in a staggered manner are arranged on the outer walls of the stirring ribs 11, the turbulent surfaces 111 can be specifically arranged in a sawtooth structure or a wave-shaped structure, the turbulent surfaces 111 are provided with at least one convex part 111a and at least one concave part 111b, the convex parts 111a and the concave parts 111b are sequentially arranged in a staggered manner along the extension direction of the stirring ribs 11, and the convex part 111a or the concave part 111b of one stirring rib 11 at the same height is arranged corresponding to the concave part 111b or the convex part 111a of the other stirring rib 11 adjacent to the convex part 111 a. So set up, can make reagent bottle 100 when horizontal rotation, the liquid in the reagent bottle 100 is similar heliciform rotation, can be better be in turbulent state, more do benefit to the mixing of reagent, strengthen the homogeneity of reagent mixing.

In a specific embodiment, two stirring ribs 11 may be oppositely disposed in the bottle body 10 of the reagent bottle 100, and the turbulent surfaces 111 of the two stirring ribs 11 may be oppositely disposed; or at least three stirring ribs 11 are arranged in the bottle body 10 around the central shaft of the bottle body 10, the turbulent surface 111 of each stirring rib 11 is arranged towards the central shaft, wherein the stirring ribs can be uniformly arranged at intervals and can also be non-uniformly arranged at intervals, the turbulent effect can be further improved by uniformly arranging at intervals, the reagent mixing is more facilitated, and the uniformity of the reagent mixing is enhanced.

In another specific embodiment, the bottom of the stirring bar 11 and the bottom of the body 10 of the reagent bottle 100 may be fixedly connected, and the two may be integrally formed and fixedly connected, or may be fixedly connected by inserting. As shown in fig. 2, in an alternative embodiment, the stirring rib 11 may be separately disposed from the inner wall of the bottle body 10 at a distance, so that the stirring rib 11 is convenient to be connected with the bottom of the bottle body 10 for disassembly, cleaning, maintenance, and the like. As shown in fig. 3, in another alternative embodiment, the stirring rib 11 may be fixedly connected to the inner wall of the bottle body 10, specifically, may be fixedly connected by integral molding, or may be fixedly connected by insertion. Connect more firmly like this, have interval components of a whole that can function independently to set up with aforementioned stirring muscle 11 and body 10 inner wall simultaneously and compare, can avoid liquid to flow in the interval between the inner wall of stirring muscle 11 and body 10 and reduce the mixing degree.

Preferably, the body 10 of the reagent bottle 100 may be formed in a cylindrical shape to facilitate horizontal rotation thereof in the reagent cartridge 200. As shown in fig. 6 and table 1 below, in order to achieve a better blending effect, the structure of the stirring rib may be further optimized, specifically, the extension height h of the stirring rib 11 may be 50% to 70% of the height of the inner cavity of the bottle body 10, and compared with the height range which is too low or too high, the turbulent flow effect is better and the reagent blending effect is better in this height range, and the test data in table 1 below shows that when the extension height h of the stirring rib 11 is 60% of the height of the inner cavity of the bottle body 10, the effect is better in comparison; furthermore, the thickness b of the stirring bar 11 is preferably 30% to 50% of the thickness of the bottle body 10, which can ensure the strength of the stirring bar 11 and the mixing effect of the reagent, and the test data in table 1 below also shows that the effect is better when the thickness b of the stirring bar 11 is 40% of the thickness of the bottle body 10; also, the width c of the stirring bar 11 is preferably 10% to 20% of the diameter of the inner cavity of the bottle body 10, and the width c of the stirring bar 11 within this range can achieve a better reagent mixing effect through experimental comparison, and it can be seen from the test data in table 1 below that, when the width c of the stirring bar 11 is 17% of the diameter of the inner cavity of the bottle body 10, the effect is better than that.

As shown in fig. 6 and table 1 below, the protruding portion 111a of the turbulent surface 111 of the stirring bar 11 may be referred to as a tooth, and the tooth may be an angle shape having a sharp end, or a rectangular shape or an arc shape having a flat surface, and in order to ensure a good kneading effect, it is preferable that the maximum protruding height a of the tooth is 60% or less of the width b of the stirring bar 11, and the tooth is preferably a triangular tooth, and in comparison, the kneading effect is best when the maximum protruding height a is 60% of the width c of the stirring bar 11.

The following provides a data reference for the mixing effect test using a magnetic chip with a large volume of 60 × 40 × 7 μm, and counting the number of magnetic beads by an interpretation device, and testing the mixing effect in the corresponding reagent bottles under the 14 protocols described in table 1 below. Firstly, magnetic bead solutions with the same concentration are respectively placed in a reagent bottle with the serial number of 1-12 and a traditional reagent bottle with the serial number of 12-14 in an isometric manner according to different stirring rib schemes, wherein the stirring ribs of the reagent bottle with the serial number of 1-11 are in a triangular shape, the maximum protrusion height a = c × 60%, and the end faces of the stirring ribs of the traditional reagent bottle with the serial number of 12-14 are in a non-concave-convex state, namely a plane; and then uniformly mixing the reagent bottles by rotating at the same parameters (the rotating speed and the duration parameters are the same), then taking the same amount of solution from each reagent bottle by using a liquid-moving device, putting the solution into interpretation equipment for counting the number of magnetic chips, performing at least 5 times of continuous tests, and calculating the average number. The theoretical number of magnetic beads in this volume of solution was 850. Table 1 below is a statistical data of the number of magnetic chips in each case solution:

table 1: magnetic chip number statistical table

Serial number

h ratio

b ratio of

c ratio of

Shape and height of stirring bar

Number of magnetic chips

Difference value

1

50％

30％

10％

Triangular teeth, height = c 60%

868

+18

2

50％

40％

17％

Triangular teeth, height = c 60%

860

+10

3

50％

20％

Triangular teeth, height = c 60%

862

+12

4

60％

30％

17％

Triangular teeth, height = c 60%

838

-12

5

60％

40％

20％

Triangular teeth, height = c 60%

840

-10

6

60％

50％

10％

Triangular tooth shape, height = c60％

834

-17

7

70％

30％

20％

Triangular teeth, height = c 60%

831

-19

8

70％

40％

10％

Triangular teeth, height = c 60%

837

-13

9

70％

50％

17％

Triangular teeth, height = c 60%

830

-20

10

60％

40％

17％

Triangular teeth, height = c 60%

843

-7

11

70％

50％

20％

Triangular teeth, height = c 60%

821

-29

12

70％

50％

20％

Plane surface

793

-57

13

60％

40％

17％

Plane surface

810

-40

14

50％

30％

10％

Plane surface

880

+30

As can be seen from the data in Table 1, the number of the magnetic chips in the scheme 10 is closest to the theoretical value, so that the relatively optimal uniform mixing effect of the magnetic chips can be achieved. And the data show that the height h of the corresponding stirring rib is 60% of the height of the inner cavity of the reagent bottle body, the thickness b of the stirring rib is 40% of the wall thickness of the reagent bottle body, and the width c of the stirring rib in the radial direction of the reagent bottle is 17% of the diameter of the inner cavity of the reagent bottle.

As shown in fig. 1 and fig. 3, the outer wall of the body 10 of the reagent bottle 100 may be provided with a fastening portion, which may be specifically configured as an annular groove 12 circumferentially disposed around the lower portion of the outer wall of the body 10 of the reagent bottle 100, and correspondingly, the reagent bottle mounting position 201 of the reagent kit 200 may be provided with a fastening protrusion 2011 that is fastened to the annular groove 12 correspondingly, where the fastening protrusion 2011 may be at least two protrusions circumferentially disposed or an annular protrusion circumferentially disposed; or conversely, the engaging portion may be specifically provided with at least two protrusions or annular protrusions circumferentially arranged around the outer wall of the body 10 of the reagent bottle 100, and correspondingly, the reagent bottle mounting portion 201 may be provided with an annular engaging groove that is engaged with the at least two protrusions or annular protrusions, and preferably, when two protrusions are provided, the two protrusions are symmetrically arranged. This kind of set up annular groove and annular bulge or with the circumference set up the mutual block of at least two protruding modes of being connected, can both play spacing block connection's effect, do not influence reagent bottle 100 again in reagent box 200 rotation mixing work. Preferably, the annular groove 12 has an annular upper top surface 121, an annular lower bottom surface and a connecting wall connecting the annular upper top surface 121 and the annular lower bottom surface, and the annular upper top surface 121 is preferably formed in an arc shape to improve the smoothness during rotation.

Further, the top of the body 10 may be circumferentially provided with a ring thread 14, and the cap 20 is screwed and fixed to the top of the body 10. Furthermore, the top of the bottle body 10 can be sequentially provided with an EP film 15 and a cross-shaped silicon film 16, the cross-shaped silicon film 16 can be arranged near the top of the bottle cap 20, the bottle cap 20 can be provided with a notch 21 corresponding to the cross-shaped silicon film 16, and the cross-shaped silicon film 16 can be exposed out of the notch 21.

Preferably, the upper portion of the bottle body 10 may be provided with a limiting portion 13 extending outward, the limiting portion 13 may be provided as a limiting protrusion, or a plurality of limiting protrusions circumferentially provided, or an outer edge of an annular protrusion circumferentially provided, and the limiting portion 13 may be integrally formed with the bottle body 10. Preferably, the limiting portion 13 may be circumferentially disposed at the bottom of the annular thread 14, and correspondingly, the top of the casing 202 of the reagent kit 200 may be correspondingly abutted against the limiting portion 13, so that the limiting portion 13 can limit the whole body 10 of the reagent bottle 100 while protecting the whole body in the casing 202 of the reagent kit 200, thereby effectively protecting the reagent bottle 100.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A reagent bottle is characterized in that a reagent bottle body is provided with a reagent bottle body,

2. The reagent bottle according to claim 1,

at least two stirring ribs are arranged in the bottle body around the central shaft of the bottle body, and the turbulent flow surface of each stirring rib is arranged towards the central shaft.

3. The reagent bottle according to claim 1 or 2,

the stirring rib is fixedly connected with the inner wall of the bottle body in an integrated forming mode, or the side wall of the stirring rib is inserted into the inner wall of the bottle body in a fixedly connected mode.

4. The reagent bottle according to claim 3,

the bottle body is cylindrical;

5. The reagent bottle according to claim 4,

the extension height of the stirring rib is 60% of the height of the inner cavity of the bottle body;

6. The reagent bottle according to claim 4 or 5,

the turbulence surface is of a sawtooth structure or a wavy structure, the convex part of the turbulence surface is in a tooth shape, the tooth shape is an angular shape with a tip, or a rectangular shape or an arc shape with a gentle surface, and the maximum protruding height of the tooth shape is less than or equal to 60% of the width of the stirring rib.

7. The reagent bottle according to claim 1,

the bottle body is cylindrical, the outer wall of the bottle body is provided with a clamping part,

8. The reagent bottle according to claim 1 or 7,

the upper portion of the bottle body extends outwards to form a limiting portion, the limiting portion is arranged to be a limiting protrusion, or a plurality of protrusions arranged on the periphery of the bottle body, or an annular protruding outer edge arranged on the periphery of the bottle body.

9. A kit, wherein the reagent bottle of any one of claims 1 to 8 is mounted in the kit, and the kit comprises a housing and a reagent bottle mounting position arranged in the housing, wherein the reagent bottle is mounted on the reagent bottle mounting position, and the reagent bottle mounting position is provided with a clamping protrusion or a clamping groove for clamping the reagent bottle;

10. A reagent storage assembly, characterized in that, includes the kit of claim 9, still includes reagent carousel and drive reagent carousel pivoted carousel drive assembly, be provided with at least one kit mounting groove along its circumference on the reagent carousel for the installation the kit, be equipped with on the kit mounting groove be used for with the first joint position with the block position of second joint position joint, the bottom of kit mounting groove still rotates with reagent mixing mechanism and is connected.