CN115260503A - Preparation method of elastic agent and application of elastic agent in microfluidic chip - Google Patents
Preparation method of elastic agent and application of elastic agent in microfluidic chip Download PDFInfo
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G77/00—Macromolecular compounds obtained by reactions forming a linkage containing silicon with or without sulfur, nitrogen, oxygen or carbon in the main chain of the macromolecule
- C08G77/42—Block-or graft-polymers containing polysiloxane sequences
- C08G77/452—Block-or graft-polymers containing polysiloxane sequences containing nitrogen-containing sequences
- C08G77/455—Block-or graft-polymers containing polysiloxane sequences containing nitrogen-containing sequences containing polyamide, polyesteramide or polyimide sequences
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- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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Abstract
The invention relates to a preparation method of an elastic agent and application thereof in a microfluidic chip, which comprises the steps of firstly preparing an amino modified PDMS substrate; carrying out self-polymerization on a DA (dopamine) solution on an amino modified PDMS substrate under an alkaline condition to form a layer of PDA film with super strong adhesion, so as to obtain a PDMS substrate modified by PDA (polydopamine); synthesizing Covalent Organic Frameworks (COFs) on the surface by a one-pot method through 'molecular welding', and obtaining a microspheric PDMS-PDA-COFs elastic agent with a microstructure which is internally and externally coated; and curing, attaching and baking the PDMS-PDA-COFs elastic agent in a mold to finally obtain the PDMS-PDA-COFs microfluidic chip. Solves the problems of the preparation of PDMS elastic agent and the application thereof in the field of microfluidic chips.
Description
Technical Field
The invention relates to a preparation method of an elastic agent, in particular to a preparation method of an elastic agent and application of the elastic agent in a microfluidic chip.
Background
The heterogeneity of individual cells in a cell population plays an important role in the development and progression of disease, but most traditional genetic analysis methods currently mask individual cell differences. Single cell sequencing can exhibit intrinsic heterogeneity of individual cells and reveal complex and rare cell populations. In recent years, different microfluidic technologies have emerged for single cell research, becoming the leading edge of the field.
Compared to conventional techniques, microfluidic techniques have several advantages in analyzing samples: firstly, the structure and the function of the microfluidic chip are designed flexibly, and the requirement of single cell analysis can be met. Second, typical microfluidic channels have dimensions of tens to hundreds of microns, and can handle solution volumes from picoliters to nanoliters, thereby reducing sample loss and high sensitivity, and enabling high throughput single cell analysis. In addition, the integration of the multifunctional unit and the microfluidic chip can realize automation, and measurement errors caused by manual operation are prevented.
PDMS is the most common chip processing material at present, is a low-temperature thermal polymerization curing polymer material, has strong moulding after molding, has certain tolerance to solvents, and has the advantages of low price, good optical transparency, good biocompatibility, air permeability, convenient operation, high efficiency and the like when used for manufacturing chips. Therefore, a series of means for detecting samples of the microfluidic chip can be developed based on the characteristics, the bonding mode of the PDMS chip is various, the operation is simple and convenient, simple reversible bonding can be realized by utilizing physical action, the chip can be repeatedly used, and irreversible bonding can also be realized by utilizing methods such as chemical modification and the like. However, the surface of PDMS has high hydrophobicity, which causes the water contact angle of the untreated surface of PDMS to be larger than 100 degrees, the buffer solution is difficult to inject, bubbles are easy to appear when the chip is cleaned, so that the experiment can not be carried out, a small amount of liquid in the channel is absorbed, and biomolecules are easy to perform nonspecific adsorption on the surface of the microchannel of the PDMS chip; third, PDMS has the disadvantages of unstable channel surface, easy deformation and collapse, etc., so it needs to be surface modified and modified for application.
Generally, a modification method of a high polymer material can be adopted, and the modification method is divided into the following steps according to the modification range: the surface modification method and the integral modification method are divided into two main categories, namely a physical modification method and a chemical modification method, wherein the surface modification method is most widely used.
Chemical modification methods can be divided into: wet modification and surface grafting by covalent bonding. The wet modification is to make the solution to be modified contact the surface of PDMS directly, so that the component to be modified on the surface of PDMS is adsorbed on the surface of PDMS by physical adsorption or electrostatic force. Common wet modification methods include layer-by-layer self-assembly, sol-gel coating, dynamic surfactant modification, protein adsorption and the like. The common characteristic of the modification methods is that the modification methods are simple overall. However, since the PDMS surface is not linked to the modification layer by covalent chemical bonds, the modification layer has poor stability and is easily lost with the increase of the usage time. The surface modification by covalent bond is to bond the modification layer to the PDMS surface by covalent bond through chemical reaction. If the modified layer is also a polymer, such surface modification is also referred to as surface grafting. The modification method has the greatest advantages that the modification layer is stable, the surface property after modification is long in retention time, and the method is a common method for chemical modification of the PDMS chip.
In the prior art, poly-dopamine (PDA) is used to modify the surface of PDMS, and dopamine is self-polymerized into poly-dopamine on the inner surface of a PDMS microfluidic chip channel under an alkaline condition to form a layer of PDA coating, thereby modifying the surface of the PDMS microfluidic chip. However, the method has certain problems, such as difficult polymerization operation in the microchannel, uneven coating thickness, unstable performance of the modified microfluidic chip, complicated preparation steps and the like.
In addition, covalent organic framework materials (COFs) are crystalline high molecular compounds formed by linking light elements through covalent bonds, and COFs have excellent properties such as hydrophilicity, high crystallinity, high stability, low mass density, good topological structure, structural diversity, and excellent conjugated structure. Thus, COFs are important candidates and hot targets for high quality surface modification. The method can be used for deposition on the surface of a solid matrix or post-synthesis of COFs particles to form a film on the surface of a substance to modify the surface of the substance, enhance the surface hydrophilic property and long-term stability, and can be used for fixing biomolecules and inhibiting the nonspecific adsorption of proteins. However, the current PDMS microfluidic chip channel surface modification method is not easy to form a stable modification layer, so that COFs have not been applied to surface modification of PDMS microfluidic chips.
Therefore, the invention provides a preparation method of the elastic agent and application of the elastic agent in a microfluidic chip.
Disclosure of Invention
The invention provides a preparation method of an elastic agent and application thereof in a microfluidic chip, and solves the problem of application of PDMS in the field of microfluidic chips.
The invention adopts the following technical scheme: a method of preparing an elastomer comprising the steps of:
(1) Performing PDA modification on the amino modified PDMS substrate: modification of PDA on amino modified PDMS substrate: dissolving absolute ethyl alcohol and strong ammonia water in deionized water, fully dissolving the absolute ethyl alcohol and the strong ammonia water by magnetic stirring to obtain a solution B, dissolving dopamine hydrochloride (DA) in the deionized water, quickly injecting the solution B, and keeping the magnetic stirring for 24 hours to obtain a PDA suspension; pouring PDA (polydopamine) suspension into the amino modified PDMS substrate obtained in the step 1, pouring 1-2mL of the suspension, uniformly mixing, and reacting at 25 ℃ for 24h; because the surface energy of PDMS is lower, through Schiff base reaction or Michael addition reaction, PDA can be uniformly coated on the surface of amino modified PDMS; washing DA (dopamine) which is not coated on PDMS (polydimethylsiloxane) for a plurality of times by using deionized water, and modifying the PDMS substrate by using PDA (polydopamine);
(2) Preparing a PDMS-PDA-COFs elastic agent: dissolving p-phenylenediamine (Pa) in 50ml of deionized water to obtain a solution A; dissolving 1,3, 5-trimethylphloroglucinol (Tp) in 50ml of ethanol to obtain a solution B; and pouring the solution A into a PDMS (polydimethylsiloxane) substrate modified by PDA (poly dopamine) to carry out Schiff base condensation reaction for 2 hours by using a one-pot method, then adding the solution B to carry out Schiff base condensation reaction to promote COFs to be coated on the surface of the PDMS substrate modified by PDA (poly dopamine) through 'molecular welding', and placing the PDMS-PDA-COFs in an environment at 37 ℃ for acting for 3 hours to obtain the PDMS-PDA-COFs elastic agent. Inspiration is initiated from macroscopic metal welding, and molecular welding refers to an integrated process of preparing a material with accuracy and no defects by using a coupling chemical environment of two materials and an interface reaction on a microscopic level between molecules.
Further, the wavelength of the ultraviolet light in the step (1) is 365nm, and the effective distance is 10cm.
Further, in the step (1), the mass and dosage ratio of the beta-mercaptoethylamine to the benzoin dimethyl ether to the alkenyl-containing PDMS substrate is 1:0.05:6.
further, the volume ratio of the absolute ethyl alcohol to the concentrated ammonia water in the step (2) is 1:1.
further, the mass ratio of the PDA (polydopamine) suspension to the amino modified PDMS substrate in the step (2) is 1:6.
further, the COFs are formed by performing Schiff base condensation reaction on monomer 1,3, 5-trimethylphloroglucinol and monomer 3,3' -dinitrobenzidine in a mass ratio of 1.
The application of the elastic agent in the microfluidic chip comprises the following steps:
(1) Preparing PDMS-PDA-COFs with microchannels: mixing a PDMS-PDA-COFs (polydimethylsiloxane-PDA-COFs) elastic agent and a curing agent according to a mass ratio of 10 to prepare a mixed solution, pouring the mixed solution into a silicon wafer male mold, pouring 0.5-1ml of the mixed solution into the silicon wafer male mold, placing the silicon wafer male mold into a baking oven, baking the silicon wafer male mold for 2 hours at 90 ℃, taking out the silicon wafer male mold, and cooling the silicon wafer male mold at room temperature to solidify PDMS-PDA-COFs; stripping to obtain PDMS-PDA-COFs with microchannels;
(2) Preparing a PDMS-PDA-COFs microfluidic chip: mixing a PDMS-PDA-COFs elastic agent and a curing agent according to a mass ratio of 10.
Further, in the step (2), the curing agent is DBP (dibutyl phthalate).
Further, the cooling time in the step (2) is 1.5-2.5 h.
Compared with the prior art, the preparation method of the PDMS-PDA-COFs micro-fluidic chip for single cell sequencing has the following beneficial effects:
(1) Preparing a PDMS-PDA-COFs elastic agent by using a PDA template guide method, wherein the PDA can guide the assembly of PDMS and COFs and coat the COFs; the PDA is used for modifying the PDMS substrate material, and the DA can be self-polymerized on the surface of PDMS particles in a solvent environment to form a layer of PDA film with super strong adhesion. In addition, active free Radicals (ROS) generated by the PDA in the polymerization process are favorable for nucleophilic reaction, in addition, the ortho position of the phenolic hydroxyl group of the PDA can react with hydroxyl or sulfydryl, amino can capture and chelate metal ions to serve as a nuclear growth site, strong adhesive force and secondary reaction capacity are provided for the subsequent modification of the PDMS surface, the PDA can serve as a secondary platform, the COFs ligand is promoted to grow on the particle surface in a molecular welding mode through a one-pot method, the defect easily generated when the COFs are coated is eliminated, the COFs are prevented from self-nucleating, and the uniform and stable coating of the COFs on the PDMS surface is realized.
(2) The integrated method is adopted to cast the PDMS with the modified surface to prepare the micro-fluidic chip, and the preparation method is simple and convenient.
(3) The surface of PDMS containing amino is obtained by the reaction of a 'click chemistry' method of inducing sulfydryl and olefin by ultraviolet irradiation and cysteamine, and PDA can be successfully grafted to the amino modified PDMS substrate by Schiff base reaction/Michael addition reaction under the room temperature alkaline condition so as to ensure the uniform coating of PDA. (4) The coating microstructure ensures the stability of performance, and the homogeneous and ordered PDMS-PDA-COFs elastic agent is formed in the coating microsphere structure, so that the structural instability caused by uneven coating due to macroscopic surface treatment can be avoided.
Drawings
FIG. 1 is a schematic view of the microstructure of PDMS-PDA-COFs material of the preparation method of PDMS-PDA-COFs microfluidic chip for single cell sequencing according to the present invention;
FIG. 2 is a schematic diagram of a chip channel of a microfluidic chip of the preparation method of the PDMS-PDA-COFs microfluidic chip for single cell sequencing according to the present invention.
Detailed Description
To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be made with reference to the accompanying drawings and preferred embodiments.
The following detailed description of the specific implementation steps is made in conjunction with the accompanying drawings:
example one
A preparation method of an elastic agent is characterized by comprising the following steps:
(1) Performing PDA modification on the amino modified PDMS substrate: modification of PDA on amino modified PDMS substrate: dissolving absolute ethyl alcohol and concentrated ammonia water in deionized water, fully dissolving the absolute ethyl alcohol and the concentrated ammonia water by magnetic stirring to obtain a solution B, dissolving dopamine hydrochloride (DA) in the deionized water, quickly injecting the solution B, and keeping the magnetic stirring for 24 hours to obtain a PDA suspension; pouring PDA (polydopamine) suspension into the amino modified PDMS substrate obtained in the step 1, pouring the mixture into a volume of 1mL, uniformly mixing, and then reacting for 24h at 25 ℃; because the surface energy of PDMS is lower, through Schiff base reaction or Michael addition reaction, PDA can be uniformly coated on the surface of amino modified PDMS; washing DA (dopamine) which is not coated on PDMS (polydimethylsiloxane) for a plurality of times by using deionized water, and modifying the PDMS substrate by using PDA (polydopamine);
(2) Preparing a PDMS-PDA-COFs elastic agent: p-phenylenediamine (Pa) is dissolved in 50ml of deionized water to obtain a solution A; dissolving 1,3, 5-trimethylphloroglucinol (Tp) in 50ml of ethanol to obtain a solution B; and pouring the solution A into a PDMS (polydimethylsiloxane) substrate modified by PDA (poly dopamine) to carry out Schiff base condensation reaction for 2 hours by using a one-pot method, then adding the solution B to carry out Schiff base condensation reaction to promote COFs to be coated on the surface of the PDMS substrate modified by PDA (poly dopamine) through 'molecular welding', and placing the PDMS-PDA-COFs in an environment at 37 ℃ for acting for 3 hours to obtain the PDMS-PDA-COFs elastic agent.
In this embodiment, the wavelength of the ultraviolet light in step (1) is 365nm, and the effective distance is 10cm.
In this embodiment, the mass ratio of the β -mercaptoethylamine, benzoin dimethyl ether, and the alkenyl group-containing PDMS substrate in step (1) is 1:0.05:6.
in this embodiment, the volume ratio of the absolute ethanol to the concentrated ammonia water in step (2) is 1:1.
in this embodiment, the mass ratio of the PDA (polydopamine) suspension in the step (2) to the amino-modified PDMS substrate is 1:6.
in this example, the COFs are formed by Schiff base condensation of a monomer 1,3, 5-trimethylphloroglucinol and a monomer 3,3' -dinitrobenzidine in a mass ratio of 1.
The application of the elastic agent in the microfluidic chip comprises the following steps:
(1) Preparing PDMS-PDA-COFs with micro-channels: mixing a PDMS-PDA-COFs (polydimethylsiloxane-PDA-COFs) elastic agent and a curing agent according to a mass ratio of 10 to prepare a mixed solution, pouring the mixed solution into a silicon wafer male mold, pouring the mixed solution with a volume of 0.5ml, placing the silicon wafer male mold into a baking oven, baking the silicon wafer male mold for 2 hours at 90 ℃, taking out the silicon wafer male mold, and cooling the silicon wafer male mold at room temperature to solidify PDMS-PDA-COFs; stripping to obtain PDMS-PDA-COFs with microchannels;
(2) Preparing a PDMS-PDA-COFs microfluidic chip: mixing a PDMS-PDA-COFs elastic agent and a curing agent according to a mass ratio of 10.
In this example, the curing agent in step (2) was DBP (dibutyl phthalate).
In this example, the cooling time in step (2) was 1.5 hours.
And (3) characterizing the contact angles of water and oil on the surface of the prepared PDMS-PDA-COFs by using a contact angle measuring instrument so as to analyze the wettability of the PDMS-PDA-COFs. The specific process is as follows: dropping a drop of 5 microliter of deionized water on the surface of PDMS-PDA-COFs, immediately taking a picture by using a water contact angle measuring instrument and calculating the water contact angle value of each material, measuring 5 different points of each material, firstly measuring that the contact angle of an unmodified PDMS microfluidic chip is 107.2 degrees, and the contact angle of the modified PDMS-PDA-COFs material is shown in Table 1, and calculating the average value as the contact angle of the material, wherein the water contact angle is 43.6 degrees, which shows that the water contact angle is greatly reduced and has hydrophilicity.
Table 1 contact Angle test
Example two
A preparation method of an elastic agent is characterized by comprising the following steps:
(1) Modification of PDA on amino modified PDMS substrate: performing PDA modification on the amino modified PDMS substrate: dissolving absolute ethyl alcohol and strong ammonia water in deionized water, fully dissolving the absolute ethyl alcohol and the strong ammonia water by magnetic stirring to obtain a solution B, dissolving dopamine hydrochloride (DA) in the deionized water, quickly injecting the solution B, and keeping the magnetic stirring for 24 hours to obtain a PDA suspension; pouring PDA (polydopamine) suspension into the amino modified PDMS substrate obtained in the step 1, pouring the mixture into a volume of 2mL, uniformly mixing, and reacting at 25 ℃ for 24h; because the surface energy of PDMS is lower, through Schiff base reaction or Michael addition reaction, PDA can be uniformly coated on the surface of amino modified PDMS; washing DA (dopamine) which is not coated on PDMS (polydimethylsiloxane) for a plurality of times by using deionized water, and modifying the PDMS substrate by using PDA (polydopamine);
(2) Preparing PDMS-PDA-COFs elastomer: p-phenylenediamine (Pa) is dissolved in 50ml of deionized water to obtain a solution A; dissolving 1,3, 5-trimethylphloroglucinol (Tp) in 50ml of ethanol to obtain a solution B; pouring the solution A into a PDMS substrate modified by PDA (poly dopamine) to carry out Schiff base condensation reaction for 2 hours by using a one-pot method, then adding the solution B to carry out Schiff base condensation reaction to promote COFs to be coated on the surface of the PDMS substrate modified by PDA (poly dopamine) through 'molecular welding', placing the PDMS substrate modified by PDA (poly dopamine) in an environment at 37 ℃, and acting for 3 hours to obtain the PDMS-PDA-COFs elastic agent.
In this embodiment, the wavelength of the ultraviolet light in step (1) is 365nm, and the effective distance is 10cm.
In this embodiment, the mass ratio of the β -mercaptoethylamine, benzoin dimethyl ether, and the alkenyl group-containing PDMS substrate in step (1) is 1:0.05:6.
in this embodiment, the volume ratio of the absolute ethanol to the concentrated ammonia water in step (2) is 1:1.
in this embodiment, the mass ratio of the PDA (polydopamine) suspension in the step (2) to the amino-modified PDMS substrate is 1:6.
in this embodiment, the COFs is formed by performing schiff base condensation reaction on a monomer 1,3, 5-trimethylenetriol and a monomer 3,3' -dinitrobenzidine in a mass ratio of 1.
The application of the elastic agent in the microfluidic chip comprises the following steps:
(1) Preparing PDMS-PDA-COFs with micro-channels: mixing a PDMS-PDA-COFs (polydimethylsiloxane-PDA-COFs) elastic agent and a curing agent according to a mass ratio of 10 to prepare a mixed solution, pouring the mixed solution into a silicon wafer male mold with a volume of 1ml, placing the silicon wafer male mold into a baking oven, baking the silicon wafer male mold for 2 hours at 90 ℃, taking out the silicon wafer male mold, and cooling the silicon wafer male mold at room temperature to solidify PDMS-PDA-COFs; obtaining PDMS-PDA-COFs with the micro-channels after stripping;
(2) Preparing a PDMS-PDA-COFs microfluidic chip: mixing a PDMS-PDA-COFs elastic agent and a curing agent according to a mass ratio of 10.
In this embodiment, the curing agent in step (2) is DBP (dibutyl phthalate).
In this example, the cooling time in step (2) was 2.5 hours.
And (3) characterizing the contact angles of water and oil on the surface of the prepared PDMS-PDA-COFs by using a contact angle measuring instrument so as to analyze the wettability of the PDMS-PDA-COFs. The specific process is as follows: a drop of 5 microliter of deionized water is dripped on the PDMS-PDA-COFs surface and the surface, a water contact angle measuring instrument is adopted to take a picture immediately and calculate the value of the water contact angle, each material is tested for 5 different points, firstly, the contact angle of an unmodified PDMS microfluidic chip is measured to be 107.2 degrees, the contact angle of the modified PDMS-PDA-COFs material is shown in the table 2, the average value is calculated to be the contact angle of the material, the water contact angle is 39.7 degrees, and the water contact angle is greatly reduced and has hydrophilicity.
Table 2 contact Angle test
Although the present invention has been described with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the present invention.
Claims (9)
1. A preparation method of an elastic agent is characterized by comprising the following steps:
(1) Performing PDA modification on the amino modified PDMS substrate: modification of PDA on amino modified PDMS substrate: dissolving absolute ethyl alcohol and concentrated ammonia water in deionized water, fully dissolving the absolute ethyl alcohol and the concentrated ammonia water by magnetic stirring to obtain a solution B, dissolving dopamine hydrochloride (DA) in the deionized water, quickly injecting the solution B, and keeping the magnetic stirring for 24 hours to obtain a PDA suspension; pouring PDA (polydopamine) suspension into the amino modified PDMS substrate obtained in the step 1, pouring 1-2mL of the suspension, uniformly mixing, and reacting at 25 ℃ for 24h; washing DA (dopamine) which is not coated on PDMS (polydimethylsiloxane) for a plurality of times by using deionized water, and modifying the PDMS substrate by using PDA (polydopamine);
(2) Preparing a PDMS-PDA-COFs elastic agent: p-phenylenediamine (Pa) is dissolved in 50ml of deionized water to obtain a solution A; dissolving 1,3, 5-trimethylphloroglucinol (Tp) in 50ml of ethanol to obtain a solution B; pouring the solution A into a PDMS substrate modified by PDA (poly dopamine) for reaction for 2 hours, and then adding the solution B; and (3) placing the mixture in an environment at 37 ℃ for acting for 3 hours to obtain the PDMS-PDA-COFs elastic agent.
2. The method of claim 1, wherein the ultraviolet light in step (1) has a wavelength of 365nm and an effective distance of 10cm.
3. The method for preparing the elastomer according to claim 1, wherein the mass ratio of the beta-mercaptoethylamine to the benzoin dimethyl ether to the alkenyl group-containing PDMS substrate in step (1) is 1:0.05:6.
4. the method for preparing the elastomer according to claim 1, wherein the volume ratio of the absolute ethanol to the concentrated ammonia water in the step (2) is 1:1.
5. the method for preparing an elastomer according to claim 1, wherein the mass ratio of the PDA (polydopamine) suspension to the amino-modified PDMS substrate in step (2) is 1:6.
6. the method of claim 1, wherein the COFs are formed by Schiff base condensation reaction of 1,3, 5-trimethylphloroglucinol monomer and 3,3' -dinitrobenzidine monomer at a mass ratio of 1.
7. Use of an elastomeric agent according to claim 1 in a microfluidic chip, characterized in that it comprises the following steps:
(1) Preparing PDMS-PDA-COFs with microchannels: mixing a PDMS-PDA-COFs (polydimethylsiloxane-PDA-COFs) elastic agent and a curing agent according to a mass ratio of 10 to prepare a mixed solution, pouring the mixed solution into a silicon wafer male mold, pouring 0.5-1ml of the mixed solution into the silicon wafer male mold, placing the silicon wafer male mold into a baking oven, baking the silicon wafer male mold for 2 hours at 90 ℃, taking out the silicon wafer male mold, and cooling the silicon wafer male mold at room temperature to solidify PDMS-PDA-COFs; obtaining PDMS-PDA-COFs with the micro-channels after stripping;
(2) Preparing a PDMS-PDA-COFs microfluidic chip: mixing a PDMS-PDA-COFs elastic agent and a curing agent according to a mass ratio of 10.
8. Use of an elastomeric agent in a microfluidic chip according to claim 7, wherein the curing agent in step (2) is DBP (dibutyl phthalate).
9. Use of an elastomer in a microfluidic chip according to claim 7, wherein the cooling time in step (2) is 1.5-2.5 h.
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| US20150132742A1 (en) * | 2012-06-01 | 2015-05-14 | President And Fellows Of Harvard College | Microfluidic Devices Formed From Hydrophobic Paper |
| CN107413313A (en) * | 2017-07-18 | 2017-12-01 | 武汉大学 | A kind of Magnetic solid phases extractant based on covalent organic framework material and its preparation method and application |
| CN109134909A (en) * | 2018-08-06 | 2019-01-04 | 浙江工业大学 | A kind of preparation method on the surface PDMS of pH response |
| CN114452683A (en) * | 2022-01-28 | 2022-05-10 | 苏州希夫安材料科技有限公司 | Silica gel/PDA/COFs three-layer composite material for oil-water separation and preparation method thereof |
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| US20150132742A1 (en) * | 2012-06-01 | 2015-05-14 | President And Fellows Of Harvard College | Microfluidic Devices Formed From Hydrophobic Paper |
| CN107413313A (en) * | 2017-07-18 | 2017-12-01 | 武汉大学 | A kind of Magnetic solid phases extractant based on covalent organic framework material and its preparation method and application |
| CN109134909A (en) * | 2018-08-06 | 2019-01-04 | 浙江工业大学 | A kind of preparation method on the surface PDMS of pH response |
| CN114452683A (en) * | 2022-01-28 | 2022-05-10 | 苏州希夫安材料科技有限公司 | Silica gel/PDA/COFs three-layer composite material for oil-water separation and preparation method thereof |
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