CN115251085A - Insecticidal fungus live spore water suspending agent and preparation method thereof - Google Patents
Insecticidal fungus live spore water suspending agent and preparation method thereof Download PDFInfo
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- CN115251085A CN115251085A CN202210824753.9A CN202210824753A CN115251085A CN 115251085 A CN115251085 A CN 115251085A CN 202210824753 A CN202210824753 A CN 202210824753A CN 115251085 A CN115251085 A CN 115251085A
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- 241000233866 Fungi Species 0.000 title claims abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000000375 suspending agent Substances 0.000 title claims abstract description 24
- 241001266001 Cordyceps confragosa Species 0.000 claims abstract description 66
- 239000000725 suspension Substances 0.000 claims abstract description 46
- 239000005667 attractant Substances 0.000 claims abstract description 31
- 230000031902 chemoattractant activity Effects 0.000 claims abstract description 29
- 239000000463 material Substances 0.000 claims abstract description 29
- 235000010493 xanthan gum Nutrition 0.000 claims abstract description 25
- 239000000230 xanthan gum Substances 0.000 claims abstract description 25
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- LROWVYNUWKVTCU-STWYSWDKSA-M sodium sorbate Chemical compound [Na+].C\C=C\C=C\C([O-])=O LROWVYNUWKVTCU-STWYSWDKSA-M 0.000 claims abstract description 19
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- 239000007900 aqueous suspension Substances 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 12
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 claims abstract description 10
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 10
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229940117916 cinnamic aldehyde Drugs 0.000 claims abstract description 10
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 229910052731 fluorine Inorganic materials 0.000 claims abstract description 10
- 239000011737 fluorine Substances 0.000 claims abstract description 10
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- 239000000661 sodium alginate Substances 0.000 claims abstract description 10
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 10
- MQIUGAXCHLFZKX-UHFFFAOYSA-N Di-n-octyl phthalate Natural products CCCCCCCCOC(=O)C1=CC=CC=C1C(=O)OCCCCCCCC MQIUGAXCHLFZKX-UHFFFAOYSA-N 0.000 claims abstract description 9
- BJQHLKABXJIVAM-UHFFFAOYSA-N bis(2-ethylhexyl) phthalate Chemical compound CCCCC(CC)COC(=O)C1=CC=CC=C1C(=O)OCC(CC)CCCC BJQHLKABXJIVAM-UHFFFAOYSA-N 0.000 claims abstract description 9
- AXMVYSVVTMKQSL-UHFFFAOYSA-N UNPD142122 Natural products OC1=CC=C(C=CC=O)C=C1O AXMVYSVVTMKQSL-UHFFFAOYSA-N 0.000 claims abstract description 8
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- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 230000002538 fungal effect Effects 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 6
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- 238000012216 screening Methods 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
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- 239000001963 growth medium Substances 0.000 claims description 3
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- 238000003912 environmental pollution Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
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- Chemical & Material Sciences (AREA)
- Dentistry (AREA)
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- Agronomy & Crop Science (AREA)
- General Chemical & Material Sciences (AREA)
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- Toxicology (AREA)
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Abstract
The invention discloses an insecticidal fungus live spore aqueous suspension, which comprises verticillium lecanii spores, a synergist, an attractant and a thickening substance, wherein the verticillium lecanii spores are verticillium lecanii conidia prepared by culturing verticillium lecanii, and the verticillium lecanii spore suspension is 2 x 10 in concentration8The insecticidal fungus with spores per ml, wherein the verticillium lecanii spore suspension also comprises the following components by weight: tween 80:1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-5 parts of sterilized water: 985-996 parts, the synergist is organic fluorine synergist, the attractant comprises cinnamic aldehyde and dioctyl phthalate, the thickening material is sodium alginate, the verticillium lecanii spore is uniformly mixed with the synergist and the attractant, the invention discloses the insecticidal fungus live spore water suspending agent and the preparation thereofThe method has the effects of slow evaporation rate of the sprayed liquid medicine, good weather resistance, and excellent permeability and conductivity.
Description
Technical Field
The invention relates to the field of plant protection, in particular to an insecticidal fungus live spore water suspending agent and a preparation method thereof.
Background
Plant insect pests are natural disasters which seriously threaten agricultural production in China. The existing method for preventing and controlling pests mainly adopts chemical pesticides, and the chemical pesticides have the advantages of quickly reducing the population density of the pests, but have obvious defects because the method simultaneously causes adverse effects of environmental pollution, killing of a large number of beneficial animals, poisoning of people and livestock, generation of drug resistance of the pests and the like.
The microbial pesticide can effectively control insect pests, does not kill natural enemies, is environment-friendly, safe to people and livestock, and has very wide application prospect. At present, the defects of high production cost, short storage period, strict requirements on the environment conditions for use, unsafe organism and the like of the microbial pesticide also limit the application range of the technology. Mainly because of the problems of low suspension rate, poor stability, easy evaporation, rapid activity reduction in normal temperature storage and the like of microbial pesticide preparations.
The invention aims at the problems to develop the aqueous suspension of the live spores of the insecticidal fungi, and effectively solves the problems.
Disclosure of Invention
The invention discloses an insecticidal fungus live spore water suspending agent and a preparation method thereof, aiming at solving the outstanding technical problems of low suspension rate, poor stability, easy evaporation, quick activity reduction of normal temperature storage and the like of a microbial pesticide preparation.
In order to achieve the purpose, the invention adopts the following technical scheme:
an insecticidal fungus live spore aqueous suspension comprises Verticillium lecanii spores, a synergist, an attractant and a thickening substance, wherein the Verticillium lecanii spores are Verticillium lecanii conidia prepared by culturing Verticillium lecanii, and the Verticillium lecanii spore suspension is 2 × 108The insecticidal fungus with spores per ml, wherein the verticillium lecanii spore suspension also comprises the following components in parts by weight: tween 80:1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-5 parts of sterilized water: 985-996 parts of an organic fluorine synergist, wherein the attractant comprises cinnamaldehyde anddioctyl phthalate, wherein the thickening substance is sodium alginate, the verticillium lecanii spores are uniformly mixed with the synergist and the attractant, the thickening substance is added after the suspending agent, and the mass ratio of the verticillium lecanii spores to the synergist to the attractant is 98:1:1, the mass ratio of the total mass of the synergist and the attractant to the mass of the verticillium lecanii spores is 1:25, the thickener accounts for 5 percent.
The verticillium lecanii spores are used as the substrate in the insecticidal fungus live spore water suspending agent, wherein the verticillium lecanii not only can host insects, but also can be used as host bacteria of plant pathogenic bacteria, the insecticidal fungus live spore water suspending agent has the characteristics of no environmental pollution, simultaneous use with certain insecticides, bactericides and acaricides and the like, meanwhile, by the arranged attractant, the pests are attracted by cinnamic aldehyde and dioctyl phthalate in the attractant, so that the pesticidal fungus live spore water suspending agent can stay on the leaf surfaces sprayed with the insecticidal fungus live spore water suspending agent for a long time, and actively eat the insecticidal fungus live spore water suspending agent in the stay process, thereby improving the insecticidal capacity and enhancing the using effect, meanwhile, the Tween 80 plays a role of dispersing the insecticidal fungus spores in the suspension, the xanthan gum can form a spore micro-outer membrane and improve the ultraviolet resistance of the spores, the specific gravity of the suspending agent can also be increased, the suspension rate of the spores is improved, the sodium sorbate is used for inhibiting the growth of bacteria, the survival rate of the insecticidal fungus spores is kept and the prevention, the generation of the leaf surfaces of the liquid medicine and the pests are good in wettability, the pesticide is good in the advantages of good chemical control and the improvement of the effect, the pesticide, the effect of the large-scale prevention and the prevention of the insect control of the pesticide are provided.
A preparation method of an insecticidal fungus live spore water suspending agent comprises the following specific steps:
s1: spore suspension preparation: preparing a spore suspension by using Verticillium lecanii;
s2: preparing auxiliary materials: preparing auxiliary materials to be added for later use;
s3: and (3) mixing auxiliary materials: mixing part of the prepared auxiliary materials with the spore suspension uniformly;
s4: and (3) post-treatment: adding a thickening material to the mixed material;
in S1, the preparation of the spore suspension comprises the following specific steps:
s11: and (3) strain culture: selecting Verticillium lecanii and culturing;
s12: vessel sterilization: soaking all containers for strain culture in 75% alcohol;
s13: washing and filtering: washing the cultured strains with purified water, and filtering with a filter screen during washing to obtain washing liquid which is conidia of Verticillium lecanii;
s14: mixing Tween 80: weighing a certain amount of 1 ml of Tween 80 and mixing with a certain amount of conidia of the insecticidal fungus;
s15: preparing a xanthan gum solution: weighing a certain amount of xanthan gum, adding a proper amount of boiled water, and stirring until the xanthan gum is completely dissolved;
s16: mixing materials: adding 0.1% Tween 80 insecticidal fungal spore suspension into xanthan gum solution, and mixing to obtain mixed solution;
s17: adding sodium sorbate: adding a certain amount of sodium sorbate;
in S1, the viable spore content in the spore suspension preparation was 2 x 108Spore/ml, and other component ratios are as follows: tween: 1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-6 parts, and the balance of water to make up to 1 liter;
in said S1, the insecticidal fungal spore content in the spore suspension preparation is 2 x 108Spore/ml, and other component ratios are as follows: tween 80:1 part; 3 parts of xanthan gum; sodium sorbate: 3 parts, and the balance of water;
in S11, the strain culture comprises the following specific steps:
s111: activating strains: preparing verticillium lecanii strains in batches, and activating the strains at normal temperature;
s112: selecting strains: performing preliminary experiments on the activated strains to screen out high-quality strains;
s113: transfer culture: transferring the screened high-quality strains to a set culture bin for further culture;
in S112, the strain selection screening step includes: inoculating each detected strain in the same culture medium for the same inoculation time and the same culture time, regularly observing the growth and drawing the corresponding position of the tube wall at the front end of the hypha every day, measuring the growth degree of the hypha after one week, calculating the average growth speed of each strain, and remaining the transfer culture when the growth speed reaches a preset value, wherein the transfer culture does not reach the preset value for elimination.
The high-quality strains are selected by screening and comparing the batches of strains, and are subjected to transfer culture, so that the quality of the final finished product of the insecticidal fungus live spore suspension concentrate is ensured, and the quantity of live spores of the same amount of strains can be increased, so that the demand of the strains is reduced, and the cost is reduced.
In a preferred scheme, in S3, the mixing of the auxiliary materials includes the following specific steps:
s31: adding a synergist: adding an organic fluorine synergist into a spore suspension prepared from verticillium lecanii;
s32: adding an attractant: adding cinnamic aldehyde and dioctyl phthalate into spore suspension prepared from Verticillium lecanii;
in S4, the post-processing includes the following specific steps:
s41: impurity filtration: filtering impurities in the mixed liquid by using a precision filter screen;
s42: adding a thickening substance: adding sodium alginate, and mixing;
s43: packaging: and packaging and storing according to the uniform weight.
Through being provided with the thickening material, the in-process of mixing with water when using, sodium alginate and water contact can produce certain viscosity to the absorption synergist, the attractant strengthen with the mixed effect of spore suspension, also strengthen the viscidity with the plant leaf surface simultaneously, slow down the speed of volatilizing, through adding the machine fluorine synergist simultaneously, also strengthen the ductility of ropy mixed liquid on the leaf surface.
From the above, the insecticidal fungus live spore aqueous suspension comprises Verticillium lecanii spores, a synergist, an attractant and a thickening substance, wherein the Verticillium lecanii spores are Verticillium lecanii conidia prepared by culturing Verticillium lecanii, and the Verticillium lecanii spore suspension has the concentration of 2 × 108The insecticidal fungus with spores per ml, wherein the verticillium lecanii spore suspension also comprises the following components in parts by weight: tween 80:1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-5 parts of sterilized water: 985-996 parts, wherein the synergist is an organic fluorine synergist, the attractant comprises cinnamic aldehyde and dioctyl phthalate, the thickening substance is sodium alginate, verticillium lecanii spores, the synergist and the attractant are uniformly mixed, the thickening substance is added after the suspending agent, and the mass ratio of the verticillium lecanii spores, the synergist and the attractant is 98:1:1, the mass ratio of the total mass of the synergist and the attractant to the mass of the verticillium lecanii spores is 1:25, the thickener accounts for 5 percent. The insecticidal fungus live spore water suspension agent and the preparation method thereof provided by the invention have the technical effects of low evaporation speed of liquid medicine after spraying, good weather resistance, and excellent permeability and conductivity.
Drawings
FIG. 1 is an overall flow chart of an insecticidal fungus live spore aqueous suspension and a preparation method thereof.
FIG. 2 is a flow chart of spore suspension preparation of an insecticidal fungus live spore aqueous suspension and a preparation method thereof.
FIG. 3 is a strain culturing flow chart of the insecticidal fungus live spore water suspending agent and the preparation method thereof.
FIG. 4 is a flow chart of the auxiliary material mixing process of the insecticidal fungus live spore water suspending agent and the preparation method thereof.
FIG. 5 is a post-treatment flow chart of the insecticidal fungus live spore aqueous suspension and the preparation method thereof.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The invention discloses an insecticidal fungus live spore water suspending agent and a preparation method thereof, which are mainly applied to pesticide spraying scenes.
Referring to fig. 1 to 5, an insecticidal fungus live spore aqueous suspension comprises verticillium lecanii spores, a synergist, a suction agent and a thickening substance, wherein the verticillium lecanii spores are verticillium lecanii conidia prepared by culturing verticillium lecanii, and the verticillium lecanii spore suspension has a concentration of 2 × 108The insecticidal fungus with spores per ml, the verticillium lecanii spore suspension also comprises the following components by weight: tween 80:1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-5 parts of sterilized water: 985-996 parts, the synergist is an organic fluorine synergist, the attractant comprises cinnamic aldehyde and dioctyl phthalate, the thickening substance is sodium alginate, verticillium lecanii spores, the synergist and the attractant are uniformly mixed, the thickening substance is added after the suspending agent, the mass ratio of the verticillium lecanii spores to the synergist to the attractant is 98:1:1, the mass ratio of the total mass of the synergist and the attractant to the mass of the verticillium lecanii spores is 1:25, the proportion of the thickening material is 5 percent.
Referring to fig. 1, a preparation method of an insecticidal fungus live spore aqueous suspension comprises the following specific steps:
s1: spore suspension preparation: preparing spore suspension by using Verticillium lecanii;
s2: preparing auxiliary materials: preparing auxiliary materials to be added for later use;
s3: mixing auxiliary materials: mixing part of the prepared auxiliary materials with the spore suspension uniformly;
s4: and (3) post-treatment: adding a thickening material to the mixed material.
Referring to fig. 2, in a preferred embodiment, in S1, spore suspension preparation comprises the following specific steps:
s11: and (3) strain culture: selecting Verticillium lecanii and culturing;
s12: vessel sterilization: soaking all containers for strain culture in 75% alcohol;
s13: washing and filtering: washing the cultured strains with purified water, and filtering with a filter screen during washing to obtain washing liquid of Verticillium lecanii conidia;
s14: mixed tween 80: weighing a certain amount of 1 ml of Tween 80 and mixing with a certain amount of conidia of the insecticidal fungus;
s15: preparing a xanthan gum solution: weighing a certain amount of xanthan gum, adding a proper amount of boiled water, and stirring until the xanthan gum is completely dissolved;
s16: mixing materials: adding 0.1% Tween 80 insecticidal fungal spore suspension into xanthan gum solution, and mixing to obtain mixed solution;
s17: adding sodium sorbate: adding a certain amount of sodium sorbate.
Referring to fig. 2, in a preferred embodiment, in S1, the viable spore content in the spore suspension preparation is 2 x 108Spore/ml, and other component ratios are as follows: tween: 1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-6 parts, and the balance being water to make up to 1 liter.
Referring to fig. 2, in a preferred embodiment, in S1, the insecticidal fungal spore content in the spore suspension preparation is 2 x 108Spore/ml, and other component ratios are as follows: tween 80:1 part; 3 parts of xanthan gum; sodium sorbate: 3 parts, and the balance water.
Referring to fig. 3, in a preferred embodiment, in S11, the strain cultivation comprises the following specific steps:
s111: activating strains: preparing verticillium lecanii strains in batches, and activating the strains at normal temperature;
s112: selecting strains: performing preliminary experiments on the activated strains, and screening out high-quality strains;
s113: transfer culture: transferring the screened high-quality strains to a set culture bin for further culture.
Referring to fig. 3, in a preferred embodiment, in S112, the strain selection screening step is: the method comprises the steps of inoculating each detected strain in the same culture medium for the same inoculation time and the same culture time, regularly observing growth and marking at positions corresponding to the pipe wall of the hypha tip every day, measuring the growth degree of the hypha after one week, calculating the average growth speed of each strain, remaining transfer culture when the growth speed reaches a preset value, excluding the preset value, screening and comparing batches of strains to select high-quality strains, and transferring and culturing the high-quality strains, so that the quality of the finished product of the final insecticidal fungus live spore water suspending agent is guaranteed, the number of live spores of the same amount of strains can be increased, the required number of the strains is reduced, and the cost is reduced.
Referring to fig. 4, in a preferred embodiment, in S3, the auxiliary material mixing includes the following specific steps:
s31: adding a synergist: adding an organic fluorine synergist into a spore suspension prepared from verticillium lecanii;
s32: adding an attractant: cinnamic aldehyde and dioctyl phthalate were added to spore suspensions prepared from Verticillium lecanii.
Referring to fig. 5, in a preferred embodiment, in S4, the post-processing includes the following specific steps:
s41: impurity filtration: filtering impurities in the mixed liquid by using a precision filter screen;
s42: adding a thickening substance: adding sodium alginate, and mixing;
s43: packaging: pack and the storage according to unified weight, through being provided with thickening material, the in-process that mixes with water when using, sodium alginate and water contact can produce certain consistency to the absorption synergist, the mixed effect of attractant enhancement and spore suspension, also strengthen the viscidity with the plant leaf surface simultaneously, slow down the speed of volatilizing, simultaneously through adding the machine fluorine synergist, also strengthen the ductility of ropy mixed solution on the leaf surface.
The working principle is as follows: when in use, the verticillium lecanii spores are used as the substrate in the insecticidal fungus living spore water suspending agent, wherein the verticillium lecanii can be used not only as host insects but also as host bacteria of plant pathogenic bacteria, has the characteristics of no environment pollution, simultaneous use with certain insecticides, bactericides and acaricides and the like, and simultaneously, by the arranged attractants, the pests are attracted by the cinnamaldehyde and the dioctyl phthalate in the attractants, so that the pests can stay on the leaf surfaces sprayed with the insecticidal fungus living spore water suspending agent for a long time and actively eat the insecticidal fungus living spore water suspending agent in the staying process, thereby improving the insecticidal capacity and enhancing the use effect, meanwhile, tween 80 plays a role in dispersing insecticidal fungal spores in suspension, xanthan gum can form a spore micro-outer membrane and improve the ultraviolet resistance of the spores, the specific gravity of a suspending agent can be increased, the suspension rate of the spores is improved, sodium sorbate is used for inhibiting bacterial growth, the survival rate of the insecticidal fungal spores is maintained, peculiar smell is prevented, the wettability of leaf surfaces and pests of liquid medicine is good, the evaporation speed of the liquid medicine after spraying is low, the weather resistance is good, the permeability and the conductivity are excellent, compared with chemical pesticides, the pesticide composition is safer, the insect killing effect is better, the control effect is improved, the cost is reduced, technical support is provided for large-scale production and application, and the pesticide composition has important significance for improving the technical level of pest control in China.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. An insecticidal fungus live spore aqueous suspension is characterized by comprising Verticillium lecanii spores, a synergist, an attractant and a thickening substance, wherein the Verticillium lecanii spores are Verticillium lecanii conidia prepared by culturing Verticillium lecanii, and the Verticillium lecanii spore suspension is 2 x 10 in concentration8The insecticidal fungus with spores per ml, wherein the verticillium lecanii spore suspension also comprises the following components in parts by weight: tween 80:1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-5 parts of sterilized water: 985-996 parts of organic fluorine synergist, wherein the attractant comprises cinnamic aldehyde and dioctyl phthalate, and the thickening materialThe verticillium lecanii powder is sodium alginate, the verticillium lecanii spores are uniformly mixed with the synergist and the attractant, the thickening substance is added after the suspending agent, and the mass ratio of the verticillium lecanii spores to the synergist to the attractant is 98:1:1, the mass ratio of the total mass of the synergist and the attractant to the mass of the verticillium lecanii spores is 1:25, the proportion of the thickener is 5 percent.
2. A preparation method of the insecticidal fungus live spore aqueous suspension agent is applied to the insecticidal fungus live spore aqueous suspension agent, and is characterized by comprising the following specific steps:
s1: spore suspension preparation: preparing spore suspension by using Verticillium lecanii;
s2: preparing auxiliary materials: preparing auxiliary materials to be added for later use;
s3: mixing auxiliary materials: mixing part of the prepared auxiliary materials with the spore suspension uniformly;
s4: and (3) post-treatment: adding a thickening material to the mixed material.
3. The method for preparing the insecticidal fungus live spore aqueous suspension agent according to claim 2, wherein in the S1, the preparation of the spore suspension comprises the following specific steps:
s11: and (3) strain culture: selecting Verticillium lecanii and culturing;
s12: vessel sterilization: soaking all containers for strain culture in 75% alcohol;
s13: washing and filtering: washing the cultured strains with purified water, and filtering with a filter screen during washing to obtain washing liquid which is conidia of Verticillium lecanii;
s14: mixing Tween 80: weighing a certain amount of 1 ml of Tween 80 and mixing with a certain amount of conidia of the insecticidal fungus;
s15: preparing a xanthan gum solution: weighing a certain amount of xanthan gum, adding a proper amount of boiled water, and stirring until the xanthan gum is completely dissolved;
s16: mixing materials: adding 0.1% Tween 80 insecticidal fungal spore suspension into xanthan gum solution, and mixing to obtain mixed solution;
s17: adding sodium sorbate: adding a certain amount of sodium sorbate.
4. The method of claim 3, wherein the viable spore content of the spore suspension prepared in S1 is 2X 108Spore/ml, and other component ratios are as follows: tween: 1-5 parts; 1-5 parts of xanthan gum; sodium sorbate: 2-6 parts, and the balance water to make up to 1 liter.
5. The method of claim 5, wherein the spore suspension of the insecticidal fungus has a spore content of 2 x 10 in S18Spore/ml, and other component ratios are as follows: tween 80:1 part; 3 parts of xanthan gum; sodium sorbate: 3 parts, and the balance water.
6. The method for preparing an insecticidal fungus live spore aqueous suspension according to claim 1, wherein in S11, the strain cultivation comprises the following specific steps:
s111: activating strains: preparing verticillium lecanii strains in batches, and activating the strains at normal temperature;
s112: and (3) selecting strains: performing preliminary experiments on the activated strains, and screening out high-quality strains;
s113: transfer culture: transferring the screened high-quality strains to a set culture bin for further culture.
7. The method as claimed in claim 7, wherein the step of selecting strains in S112 comprises: inoculating each detected strain in the same culture medium for the same inoculation time and the same culture time, regularly observing the growth and drawing the corresponding position of the tube wall at the front end of the hypha every day, measuring the growth degree of the hypha after one week, calculating the average growth speed of each strain, and remaining the transfer culture when the growth speed reaches a preset value, wherein the transfer culture does not reach the preset value for elimination.
8. The preparation method of the insecticidal fungus live spore aqueous suspension agent according to claim 2, wherein in the step S3, the mixing of the auxiliary materials comprises the following specific steps:
s31: adding a synergist: adding an organic fluorine synergist into a spore suspension prepared from verticillium lecanii;
s32: adding an attractant: cinnamic aldehyde and dioctyl phthalate were added to spore suspensions prepared from Verticillium lecanii.
9. The method for preparing an insecticidal fungus live spore aqueous suspension agent according to claim 2, wherein in the step S4, the post-treatment comprises the following specific steps:
s41: impurity filtration: filtering impurities in the mixed liquid by using a precision filter screen;
s42: adding a thickening substance: adding sodium alginate, and mixing;
s43: packaging: and packaging and storing according to uniform weight.
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| CN101319193A (en) * | 2008-07-01 | 2008-12-10 | 华南农业大学 | Verticillium lecanii strain and uses thereof |
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| CN111657275A (en) * | 2020-07-20 | 2020-09-15 | 北京嘉景生物科技有限责任公司 | Insecticidal fungal spore aqueous suspension and preparation method thereof |
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| CN101319193A (en) * | 2008-07-01 | 2008-12-10 | 华南农业大学 | Verticillium lecanii strain and uses thereof |
| CN101658187A (en) * | 2009-09-16 | 2010-03-03 | 甘肃省科学院生物研究所 | Compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and preparation method thereof |
| CN111657275A (en) * | 2020-07-20 | 2020-09-15 | 北京嘉景生物科技有限责任公司 | Insecticidal fungal spore aqueous suspension and preparation method thereof |
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