CN115236326A - Magnetic control chip based on rapid detection of antigen and antibody and preparation method thereof - Google Patents
Magnetic control chip based on rapid detection of antigen and antibody and preparation method thereof Download PDFInfo
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Abstract
The application discloses a magnetic control chip based on rapid detection of antigen and antibody and a preparation method thereof. The magnetic control chip based on the rapid detection of the antigen antibody comprises an upper chip plate, a lower chip plate and a flow channel formed between the upper chip plate and the lower chip plate, wherein the upper chip plate is provided with a flow channel inlet for allowing a magnetic marking antigen antibody modification solution to flow into the flow channel and a liquid storage area communicated with the outlet end of the flow channel; and the upper chip plate and/or the lower chip plate are/is provided with a magnet capturing structure which is arranged corresponding to the position of the flow channel, and the magnet capturing structure is suitable for capturing magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel and enriching the magnetic beads on the flow channel. Due to the arrangement of the magnet capturing structure, magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel can be captured and enriched on the flow channel, so that the capturing efficiency can be improved, and the detection sensitivity can be improved.
Description
Technical Field
The application relates to the technical field of antigen-antibody detection, in particular to a magnetic control chip based on antigen-antibody rapid detection and a preparation method thereof.
Background
The antigen-antibody combination has high specificity and stability, and can be used for detecting microorganism or macromolecule products and calibrating cell surface protein. Commonly used antigen-antibody detection techniques include immunofluorescence labeling, radioimmunoassay, and enzyme-linked immunosorbent assay. Because the cost of the fluorescence immune labeling method and the radioimmunoassay method is higher, the prior common antigen-antibody rapid detection method usually takes an enzyme-linked immune labeling method as the main method.
The detection method aiming at the COVID-19 at present adopts an antigen-antibody detection method based on a test strip, such as a colloidal gold method, a latex method and the like, the antigen-antibody detection method is similar to an enzyme-linked immunosorbent assay method, an antigen and a specific antibody are combined on a combination pad, and a specific antibody of an antigen to be detected is modified on a detection line of the test strip.
However, the test strip-based antigen-antibody detection scheme has the following disadvantages:
the moving liquid under the action of capillary force on the test strip drives the antigen or antibody to move at a high speed, and the probability of being captured at the detection line or quality control line carried by the nitrocellulose membrane is low, so that the rapid detection scheme of the antigen and the antibody based on the test strip has the problems of low sensitivity and insufficient capture efficiency, the color development intensity is weakened, and the common weak positive is difficult to detect.
Therefore, there is a need for a new antigen-antibody detection device.
Content of application
Therefore, the present application is to solve the technical problem of overcoming the defects of low sensitivity and low capture efficiency of the antigen-antibody detection device in the prior art, so as to provide a magnetic control chip based on rapid antigen-antibody detection with high sensitivity and high capture efficiency and a manufacturing method thereof.
In order to solve the technical problem, the technical scheme of the application is as follows:
a magnetic control chip based on rapid detection of antigen and antibody comprises an upper chip plate, a lower chip plate and a flow channel formed between the upper chip plate and the lower chip plate, wherein the upper chip plate is provided with a flow channel inlet for allowing a magnetic marking antigen and antibody modification solution to flow into the flow channel and a liquid storage area communicated with the outlet end of the flow channel; and the upper chip plate and/or the lower chip plate are/is provided with a magnet capturing structure which is arranged corresponding to the position of the flow channel, and the magnet capturing structure is suitable for capturing magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel and enriching the magnetic beads on the flow channel.
Further, the magnet capture structure comprises a magnet and a soft magnetic strip arranged corresponding to the magnet, wherein the soft magnetic strip is suitable for adsorbing the magnetic beads.
Further, one side of the chip lower plate, which faces away from the chip upper plate, is provided with a containing groove, the magnet capturing structure comprises a chip back plate which is detachably connected and arranged on the containing groove, a clamping groove and a pasting area which is correspondingly arranged at the position of the clamping groove are arranged on the chip back plate, the magnet is arranged in the clamping groove, and the soft magnetic strip is pasted on the pasting area.
Further, the chip upper plate is a transparent plate body, and the chip lower plate is a non-transparent plate body.
Furthermore, a pressure-sensitive adhesive is arranged between the chip upper plate and the chip lower plate, and the flow channel is formed on the pressure-sensitive adhesive.
Further, the thickness of the pressure-sensitive adhesive is 80-120 μm.
The application also provides a preparation method of the magnetic control chip based on rapid detection of the antigen and the antibody, which comprises the following steps:
s1, preparing an upper chip plate and a lower chip plate; wherein, the upper plate of the chip is provided with a liquid storage area and a runner inlet for dropping the magnetic labeling antigen-antibody modifying solution;
s2, a flow channel is formed between the chip upper plate and the chip lower plate, one end of the flow channel is communicated with the flow channel inlet, and the other end of the flow channel is communicated with the liquid storage area;
and S3, assembling a magnet capturing structure on the upper chip plate and/or the lower chip plate corresponding to the position of the flow channel, wherein the magnet capturing structure is suitable for capturing magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel and enriching the magnetic beads on the flow channel.
Further, in the step S1, a transparent polymethyl methacrylate plate is taken, and the chip upper plate having the flow channel inlet and the liquid storage region is formed by milling with a micron precision milling machine; and taking a black polymethyl methacrylate plate, and milling and forming the lower chip plate by using a micron precision milling machine.
Further, in the step S2, a pressure-sensitive adhesive with a thickness of 80-120 μm is attached to the upper surface of the chip lower plate prepared in the step S1, a laser or a cutter is used to carve the flow channel in the pressure-sensitive adhesive, and the redundant pressure-sensitive adhesive is taken out; and (2) bonding the chip upper plate prepared in the step (S1) and the chip lower plate with the pressure-sensitive adhesive, and pressing the chip upper plate and the chip lower plate under the pressure of 15-25 kPa.
Further, in the step S3, a black polymethyl methacrylate plate is taken, and a chip back plate having a clamping groove and a bonding area is formed by milling with a micron precision milling machine; assembling a magnet into the card slot of the chip backplane; taking a soft magnetic strip, and attaching the soft magnetic strip to the pasting area by using a glue film with the thickness of 50-100 mu m; and clamping the chip back plate in the accommodating groove of the chip lower plate.
This application technical scheme has following advantage:
1. the application provides a magnetic control chip based on antigen antibody short-term test owing to be provided with the magnet at the runner position department of correspondence and catch the structure, when magnetism mark antigen antibody modification solution through constrictive runner, but the magnetic bead of the magnetism mark antigen antibody modification solution of high flux of magnet catch the structure and enrich in on the runner, can improve and catch efficiency, and then improve the sensitivity that detects.
2. The application provides a magnetic control chip based on antigen antibody short-term test because the magnet catches the structure and includes magnet and the soft magnetic strip that corresponds the setting with magnet, and the soft magnetic strip produces extremely strong local magnetic field intensity after being magnetized by magnet, and the magnetic bead that will flow through the runner separates from the solution and catches and the enrichment, further improves and catches efficiency.
3. The application provides a magnetic control chip based on antigen antibody short-term test because the chip upper plate is transparent, can follow the direct observation magnetic bead colour of chip upper plate, improves the convenience of detecting.
4. The application provides a magnetic control chip based on antigen antibody short-term test because magnet catches structure detachably and connects on the chip hypoplastron, so, but magnet catch structure cyclic utilization, low cost is applicable to large-scale antigen antibody short-term test.
5. The application provides a magnetic control chip based on antigen antibody short-term test because the runner adopts polymethyl methacrylate board-pressure sensitive adhesive-polymethyl methacrylate board three layer construction to seal the shaping, and assembly process is simple, stable in structure, and the preparation process is easy and low price, is applicable to mass production.
6. According to the magnetic control chip based on the rapid detection of the antigen and the antibody, the thickness of the pressure-sensitive adhesive is 80-120 microns, so that the soft magnetic strip can be guaranteed to have sufficient capture capacity on magnetic beads, and the processing convenience of a forming flow channel on the pressure-sensitive adhesive can be improved.
7. The preparation method of the magnetic control chip based on the rapid detection of the antigen and the antibody can prepare the magnetic control chip based on the rapid detection of the antigen and the antibody with the advantages.
Drawings
In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings used in the detailed description or the prior art description will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic perspective view of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 2 is a schematic sectional view of a portion of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 3 is another schematic cross-sectional view of the magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 4 is a schematic diagram of the back side of the magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 5 is a schematic front perspective view of an upper plate of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 6 is a schematic back perspective view of an upper plate of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 7 is a schematic front perspective view of a lower plate of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 8 is a schematic back perspective view of a lower plate of the magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 9 is a schematic back perspective view of a back plate of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 10 is a front perspective view of a back plate of a magnetic control chip for rapid antigen-antibody detection according to the present application;
FIG. 11 is a schematic diagram showing relative positions of soft magnetic strips of a magnet capture structure of a magnetron chip for rapid antigen-antibody detection according to the present application;
FIG. 12 is a schematic diagram showing the relative positions of the magnets of the magnet capturing structure of the magnetic control chip for rapid antigen-antibody detection according to the present application.
Description of the reference numerals:
1. an upper chip board; 2. a pressure sensitive adhesive; 3. a chip lower plate; 4. a chip backplane; 51. a flow channel inlet; 52. a flow channel; 53. a liquid storage area; 7. accommodating a tank; 8. a pasting area; 9. a card slot; 10. a soft magnetic ribbon; 11. and a magnet.
Detailed Description
The technical solutions of the present application will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and operate, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
In addition, the technical features mentioned in the different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.
Example 1
As shown in fig. 1 to 12, the present embodiment provides a magnetic control chip based on rapid antigen-antibody detection, which includes an upper chip plate 1, a lower chip plate 3, and a magnet capture structure. A flow channel 52 is formed between the upper chip plate 1 and the lower chip plate 3, and the outlet end of the flow channel 52 is communicated with a liquid storage area 53 for storing waste liquid.
The upper plate 1 of the chip is formed by milling a transparent PMMA (polymethyl methacrylate) plate by using a micron precision milling machine, a liquid storage area 53 and a flow channel inlet 51 allowing magnetic-labeled antigen-antibody modifying solution to flow into a flow channel 52 are arranged on the upper plate, the flow channel inlet 51 is communicated with the flow channel 52, the flow channel inlet 51 is positioned at the inlet end of the flow channel 52, the liquid storage area 53 is positioned at the outlet end of the flow channel 52, the magnetic-labeled antigen-antibody modifying solution flows to the liquid storage area 53 through the flow channel 52, when the chip is used, absorbent paper is placed in the liquid storage area 53, the absorbent paper can absorb the liquid in the liquid storage area 53, the liquid in the liquid storage area 53 is reduced, the pressure is reduced, the magnetic-labeled antigen-antibody modifying solution in the flow channel 52 can flow to the liquid storage area 53 quickly under the negative pressure effect, and the absorbent paper is arranged to be beneficial to improving the liquid flux in the flow channel 52, so as to improve the capture efficiency and the sensitivity of antigen-antibody detection. Of course, the PMMA plate can be replaced by other solid materials which are transparent and have hydrophilicity (or can have hydrophilicity after surface treatment), such as PC, PVC and other plates.
The chip lower plate 3 is formed by milling a black PMMA plate by a micron precision milling machine, the front surface of the chip lower plate 3 is adhered to the back surface of the pressure sensitive adhesive 2 with the thickness of 80-120 mu m, a flow channel 52 is carved on the front surface of the pressure sensitive adhesive 2 by using laser or a cutter, and the outlet end of the flow channel 52 is communicated with a liquid storage area 53. The flow channel 52 is formed by sealing a PMMA-pressure sensitive adhesive-PMMA three-layer structure, so that the assembly process is simple, the structure is stable, the preparation process is easy, the price is low, and the method is suitable for mass production. In addition, the flow path 52 may be provided through the pressure-sensitive adhesive 2 or may not be provided through the pressure-sensitive adhesive 2. And adhering the back surface of the chip upper plate 1 to the front surface of the pressure sensitive adhesive 2, and pressing the chip upper plate 1 and the chip lower plate 3 at the pressure of 15-25kPa to remove air bubbles among the chip upper plate 1, the pressure sensitive adhesive 2 and the chip lower plate 3.
The thickness setting of the pressure sensitive adhesive 2 takes into account magnetic field factors and material processing factors. If the thickness of the pressure sensitive adhesive 2 is too large, the magnetic field capturing force may be insufficient to enable efficient color development in the case where the distance from the bottom of the flow channel 52 to the soft magnetic tape 10 is more than 120 μm. If the thickness of the pressure sensitive adhesive 2 is too small, it will increase the difficulty of molding the runner 52 on the pressure sensitive adhesive 2.
Of course, the flow channel 52 can also be formed on the chip upper board 1, for example, disposed through the chip upper board 1 or disposed on the back surface of the chip upper board 1. When the flow channel 52 is provided through the chip upper plate 1, the color development effect can be observed conveniently without requiring the chip upper plate 1 to be transparent. The flow channel 52 may be formed on the front surface of the lower chip plate 3. Regardless of the arrangement, the flow channel 52 is provided to provide a flow path for the magnetically labeled antigen-antibody modifying solution.
The magnet capturing structure is disposed corresponding to the position of the flow channel 52, and the magnet capturing structure may be disposed on the lower chip plate 3 or on the upper chip plate 1, or on both the upper chip plate 1 and the lower chip plate 3. The magnet capture structure comprises a chip back plate 4, a magnet 11 and a soft magnetic strip 10 arranged corresponding to the magnet 11. Specifically, be equipped with draw-in groove 9 on the chip backplate 4, draw-in groove 9 utilizes micron order milling machine to mill, and magnet 11 is installed in draw-in groove 9, and magnet 11 can be for N42 or N52 magnet, still is equipped with pasting regional 8 on the chip backplate 4, and pasting regional 8 covers draw-in groove 9, and soft magnetic strip 10 pastes in pasting regional 8, and the long limit of soft magnetic strip 10 and magnet 11 aligns, and soft magnetic strip 10 is iron-cobalt-nickel alloy or iron-nickel alloy amorphous strip, for example: 1J36, 4J44, 4J46, etc. Because the soft magnetic strip 10 has extremely high magnetic susceptibility, the soft magnetic strip 10 magnetized by the magnet 11 has extremely high local magnetic field strength, the change of the boundary magnetic field is concentrated, and a large number of magnetic beads can be captured in a small area. The magnet 11 is used as a magnetic field source, the soft magnetic strip 10 is used as a magnetic field regulation and control mode, and the magnet 11 and the soft magnetic strip are matched to generate a stable small-range high-strength capture magnetic field in the flow channel 52, so that magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel 52 are captured and enriched on the flow channel 52.
In the present embodiment, the length of the magnet 11 is 5mm, the height of the magnet is 3.2mm, and the width is 2mm or more, but of course, other dimensions may be used, and the selection is not limited herein, and may be adjusted according to actual needs. The thickness of the soft magnetic strip 10 is 20-50 μm, the length of the soft magnetic strip 10 is 3mm, the width is 1mm, and of course, the length of the soft magnetic strip 10 can be other sizes, and whatever the size, the length of the soft magnetic strip 10 needs to be capable of ensuring that the color development strip length of the magnetic control chip based on the antigen-antibody rapid detection is suitable for observation.
As an embodiment, the magnet capture structure is provided on the lower chip plate 3. Specifically, one side of the chip lower plate 3, which faces away from the chip upper plate 1, is provided with a holding groove 7, and the magnet capturing structure is installed in the holding groove 7.
As another embodiment, the magnet capture structure is provided on the chip upper plate 1.
As another embodiment, the magnet capture structure may be provided on both the chip upper plate 1 and the chip lower plate 3.
The working process of the magnetic control chip based on the rapid detection of the antigen and the antibody is as follows:
finishing incubation of an antigen and an antibody in a liquid environment, specifically, modifying the antibody or the antigen by virtue of magnetic beads and then performing secondary modification by virtue of chromogenic microspheres to form a magnetic labeling antigen and antibody modification solution, wherein the magnetic beads and the chromogenic microspheres are connected together by virtue of viruses, and as the antigen and the antibody are finished incubation in the liquid environment, the combination process is controllable and the combination efficiency is more stable;
dropping the magnetic labeled antigen-antibody modifying solution into the inlet 51 of the flow channel;
the magnetic labeling antigen-antibody modifying solution flows along the flow channel 52 under the suction force of the water absorption paper, in the flowing process, the soft magnetic strip 10 which is positioned below the flow channel 52 and magnetized by the magnet generates extremely strong local magnetic field intensity, magnetic beads flowing through the flow channel 52 are separated, captured and enriched from the solution, the color developing microspheres are gathered to present a specific color due to the fact that the magnetic beads are connected with the color developing microspheres, the color can be observed through the transparent chip upper plate 1, the detection result is obtained according to the color, and high-efficiency capture and high-sensitivity color development are finally achieved; certainly, the magnetic labeling antigen-antibody modification solution can also present a specific color only by depending on the color aggregation of the magnetic beads without secondary modification of the color developing microspheres;
the magnetic labeling antigen-antibody modification solution finally enters a liquid storage area 53;
after use, the magnet capture structure is taken out of the accommodating groove 7 of the lower chip plate 3 and is loaded into a new accommodating groove 7 of the lower chip plate 3, so that the magnet capture structure can be repeatedly utilized, the cost is low, and the method is suitable for large-scale rapid antigen-antibody detection.
Example 2
The embodiment provides a preparation method of a magnetic control chip based on rapid detection of antigen and antibody, which comprises the following steps:
s1, preparing an upper chip plate 1 and a lower chip plate 3; wherein, the upper plate 1 of the chip is provided with a liquid storage area 53 and a flow channel inlet 51 for dropping the magnetic labeling antigen-antibody modifying solution;
s2, a flow channel 52 is formed between the chip upper plate 1 and the chip lower plate 3, one end of the flow channel 52 is communicated with a flow channel inlet 51, and the other end of the flow channel 52 is communicated with a liquid storage area 53;
as an embodiment, the flow channel 52 is formed on the chip upper plate 1.
As another embodiment, the flow channel 52 is formed on the chip lower plate 3.
And S3, assembling a magnet capturing structure on the position, corresponding to the position of the flow channel 52, of the chip upper plate 1 and/or the chip lower plate 3, wherein the magnet capturing structure is suitable for capturing magnetic beads in the magnetic labeling antigen-antibody modifying solution flowing through the flow channel 52 and enriching the magnetic beads on the flow channel 52.
In the step S1, a transparent polymethyl methacrylate plate is taken, and a chip upper plate 1 with a runner inlet 51 and a liquid storage area 53 is formed by milling through a micron precision milling machine; the black polymethyl methacrylate plate is taken and milled by a micron precision milling machine to form the chip lower plate 3, and naturally, the polymethyl methacrylate plate can also be replaced by other insoluble transparent solid materials with hydrophilicity (or surface treatment to enable the polymethyl methacrylate plate to have hydrophilic property), such as plates of PC, PVC and the like.
In the step S2, a pressure-sensitive adhesive 2 with the thickness of 80-120 microns is attached to the upper surface of the chip lower plate 3 prepared in the step S1, a flow channel 52 is carved on the pressure-sensitive adhesive 2 by using laser or a cutter, and the redundant pressure-sensitive adhesive 2 is taken out; and (3) bonding the chip upper plate 1 prepared in the step (S1) and the chip lower plate 3 with the pressure-sensitive adhesive 2, pressing the chip upper plate 1 and the chip lower plate 3 at the pressure of 15-25kPa, and extruding bubbles.
In the step of S3, a black polymethyl methacrylate plate is taken, and a chip back plate 4 with a clamping groove 9 and a sticking area 8 is formed by milling and processing through a micron precision milling machine; assembling the magnet into the card slot 9 of the chip backboard 4; taking the soft magnetic strip 10, attaching the soft magnetic strip 10 with the thickness of 20-50 microns, the length of 3mm and the width of 1mm to a pasting area 8 of the soft magnetic strip 10 by using an adhesive film with the thickness of 50-100 microns, and aligning the long edge of 3mm of the soft magnetic strip 10 with the long edge of 5mm of the magnet; the chip back plate 4 is mounted and clamped in the accommodating groove 7 of the chip lower plate 3.
Of course, the size of the soft magnetic tape 10, the size of the magnet, the size of the adhesive film, and the size of the pressure sensitive adhesive 2 are not limited to the above values, and may be adjusted according to actual needs, and are not particularly limited herein.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of this invention are intended to be covered by the scope of the invention as expressed herein.
Claims (10)
1. The magnetic control chip based on rapid detection of the antigen and the antibody is characterized by comprising an upper chip plate (1), a lower chip plate (3) and a flow channel (52) formed between the upper chip plate (1) and the lower chip plate (3), wherein the upper chip plate (1) is provided with a flow channel inlet (51) suitable for allowing a magnetic labeling antigen and antibody modification solution to flow into the flow channel (52) and a liquid storage area (53) communicated with the outlet end of the flow channel (52); and the chip upper plate (1) and/or the chip lower plate (3) are/is provided with a magnet capturing structure which is arranged corresponding to the position of the flow channel (52), and the magnet capturing structure is suitable for capturing magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel (52) and enriching the magnetic beads on the flow channel (52).
2. The magnetic control chip for antigen-antibody based rapid detection according to claim 1, wherein the magnet capture structure comprises a magnet (11) and a soft magnetic strip (10) disposed corresponding to the magnet (11), the soft magnetic strip (10) being adapted to adsorb the magnetic beads.
3. The magnetic control chip for rapid antigen-antibody detection according to claim 2, wherein a receiving groove (7) is formed in a side of the lower chip plate (3) facing away from the upper chip plate (1), the magnet capturing structure includes a chip back plate (4) detachably connected and installed in the receiving groove (7), a clamping groove (9) and a pasting area (8) corresponding to the clamping groove (9) are formed in the chip back plate (4), the magnet (11) is installed in the clamping groove (9), and the soft magnetic strip (10) is pasted on the pasting area (8).
4. The magnetic control chip for rapid antigen-antibody detection according to claim 1, wherein the upper chip plate (1) is a transparent plate, and the lower chip plate (3) is a non-transparent plate.
5. The magnetic control chip for rapid antigen-antibody detection according to claim 1, wherein a pressure sensitive adhesive (2) is disposed between the upper chip plate (1) and the lower chip plate (3), and the flow channel (52) is formed on the pressure sensitive adhesive (2).
6. The magnetic control chip for rapid detection based on antigen and antibody according to claim 5, characterized in that the thickness of the pressure-sensitive adhesive (2) is 80-120 μm.
7. A preparation method of a magnetic control chip based on rapid detection of antigen and antibody is characterized by comprising the following steps:
s1, preparing an upper chip plate (1) and a lower chip plate (3); wherein, the upper plate (1) of the chip is provided with a liquid storage area (53) and a flow channel inlet (51) for dropping a magnetic labeling antigen-antibody modifying solution;
s2, a flow channel (52) is formed between the chip upper plate (1) and the chip lower plate (3), one end of the flow channel (52) is communicated with the flow channel inlet (51), and the other end of the flow channel (52) is communicated with the liquid storage area (53);
and S3, assembling a magnet capture structure on the chip upper plate (1) and/or the chip lower plate (3) corresponding to the position of the flow channel (52), wherein the magnet capture structure is suitable for capturing magnetic beads in the magnetic labeling antigen-antibody modification solution flowing through the flow channel (52) and enriching the magnetic beads on the flow channel (52).
8. The method for preparing a magnetic control chip based on antigen-antibody rapid detection according to claim 7, wherein in the step S1, a transparent polymethyl methacrylate plate is taken, and the upper chip plate (1) having the flow channel inlet (51) and the liquid storage region (53) is formed by milling with a micrometer precision milling machine; and (3) taking a black polymethyl methacrylate plate, and milling and forming the lower chip plate (3) by using a micron precision milling machine.
9. The method for preparing a magnetic control chip based on antigen-antibody rapid detection according to claim 7, wherein in the step S2, a pressure sensitive adhesive (2) with a thickness of 80-120 μm is attached to the upper surface of the chip lower plate (3) prepared in the step S1, the runner (52) is engraved on the pressure sensitive adhesive (2) by using laser or a cutter, and the pressure sensitive adhesive (2) is taken out; and (3) attaching the chip upper plate (1) prepared in the step (S1) and the chip lower plate (3) with the pressure-sensitive adhesive (2), and pressing the chip upper plate (1) and the chip lower plate (3) at the pressure of 15-25 kPa.
10. The method for preparing a magnetic control chip based on antigen-antibody rapid detection according to claim 7, wherein in the step S3, a black polymethyl methacrylate plate is taken, and a chip back plate (4) with a slot (9) and a pasting region (8) is formed by milling with a micron precision milling machine; assembling a magnet (11) into the card slot (9) of the chip backplane (4); taking a soft magnetic strip (10), and attaching the soft magnetic strip (10) to the pasting area (8) by using a glue film with the thickness of 50-100 mu m; and clamping the chip back plate (4) in the accommodating groove (7) of the chip lower plate (3).
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