CN115219656A - Device for marking in-situ collection of root exudates and rhizosphere soil - Google Patents
Device for marking in-situ collection of root exudates and rhizosphere soil Download PDFInfo
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- CN115219656A CN115219656A CN202210717789.7A CN202210717789A CN115219656A CN 115219656 A CN115219656 A CN 115219656A CN 202210717789 A CN202210717789 A CN 202210717789A CN 115219656 A CN115219656 A CN 115219656A
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- 210000000416 exudates and transudate Anatomy 0.000 title claims abstract description 30
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 22
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 239000004677 Nylon Substances 0.000 claims abstract description 8
- 229920001778 nylon Polymers 0.000 claims abstract description 8
- 230000028327 secretion Effects 0.000 claims description 8
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- 230000008635 plant growth Effects 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0243—Protective shelters for young plants, e.g. tubular sleeves
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/02—Treatment of plants with carbon dioxide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/004—CO or CO2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The invention discloses a device for marking in-situ collection of root exudates and rhizosphere soil, which comprises a top cover for simulating the ground and a root box for simulating the underground, wherein the root box is filled with soil, and the top cover is buckled above the root box; the top cover is a closed transparent space, an air inlet and an air outlet are arranged on the top cover, and the air inlet is used for filling the top cover 13 CO 2 The air outlet of the air receiving switching port is used for controlling the inside of the top cover 13 CO 2 Concentration; root boxThe inner part of the test seedling box is provided with a plurality of root system columns and rhizosphere nets for placing the roots of the test seedlings, the root system columns are cylindrical tubes, and root tips are arranged at pipe orifices above the root system columns; a collecting cavity for collecting root exudates is also arranged below the root box and is positioned below the root system column; the rhizosphere net is a double-layer nylon net fixed at an angle of 45 degrees, and part of root systems grow in the space.
Description
Technical Field
The invention relates to the technical field of experimental equipment, in particular to a device for marking in-situ collection of root exudates and rhizosphere soil.
Background
Root exudates refer to chemical substances secreted or released from different parts of the root system into the growth medium during the growth of plants. The root exudates are not only considerable in quantity and various in variety, but also are affected by the growing environment, and the variety and relative amount of the root exudates are changed in a complex way. Meanwhile, root exudates are used as nutrients of soil microorganisms, and Stable isotope nucleic acid probe technology DNA-SIP (Stable isotope nucleic acid probe) is a powerful tool for coupling and analyzing plant metabolites, microorganism species composition and physiological functions thereof in a complex environment.
Isotope labeling of plants and rhizosphere microorganisms thereof, collecting of root exudates and rhizosphere microorganisms must be collected in situ in the same system, but most of the collection of the same space and the same time of the root exudates and the rhizosphere microorganisms of the same system can only be destructively sampled, and isotope labeling is mostly micro universe or seedling stage experiments, and meanwhile, an experimental device combining an isotope labeling method is rarely reported. Therefore, in order to overcome the defects, the invention needs to invent an in-situ collection device for DNA-SIP-root secretion-rhizosphere microorganisms, which is in situ, convenient and effective.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a device for marking in-situ collection of root exudates and rhizosphere soil, so as to solve the problems mentioned in the background technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
a device for collecting root exudates and rhizosphere soil in situ by marking comprises a top cover used for simulating the ground and a root box used for simulating the underground, wherein the root box is filled with soil, and the top cover is buckled above the root box; the top cover is a closed transparent space, an air inlet and an air outlet are arranged on the top cover, and the air inlet is used for filling the top cover 13 CO 2 The air outlet is used for controlling the inside of the top cover 13 CO 2 Concentration; a plurality of root system columns for placing roots of test seedlings are arranged inside the root box, the root system columns are cylindrical tubes, and root tips are arranged at pipe orifices above the root system columns; the below of root case still is equipped with the collection chamber that is used for collecting the root system secretion, it is located the below of root system post to collect the chamber.
Furthermore, the air inlet is arranged at the upper part of the top cover, the air outlet is arranged at the lower part of the top cover, and the position of the air inlet is higher than that of the air outlet.
Further, the up end of root case is root case top cap, root case top cap is separated overhead guard and root case and is placed the overhead guard, set up the vegetation mouth and the vegetation mouth that supply the examination seedling to pass on the root case top cap and be located the overhead guard.
Furthermore, a sealing film is arranged at the top cover of the root box to ensure that the root box is arranged in the top cover 13 CO 2 Can not enter the root box through the gas circulation.
Furthermore, the top cover of the root box is of a split structure so as to operate and water the root box.
Furthermore, an air vent used for ensuring the air circulation of the soil in the root box is further formed in the top cover of the root box, and the air vent is located outside the top cover.
Furthermore, the root net for root system climbing growth is further arranged, the root net is a plane formed by fixing two layers of nylon nets through an acrylic rod, the nylon nets are obliquely arranged at an angle of 45 degrees, and partial root systems grow on the root net.
Further, the upper pipe mouth department of collecting the chamber is equipped with the separation net that is used for the interior soil of separation root case to drop, the separation net adopts 100 mesh nylon wire.
Furthermore, the inside of the top cover is provided with CO 2 Real time concentration display of said CO 2 The real-time concentration display is arranged at the top of the top cover.
Furthermore, the root box and the top cover are both made of transparent acrylic materials.
Compared with the prior art, the invention has the following beneficial effects:
compared with the traditional DNA-SIP device, the device is suitable for isotopic labeling of plants with different appearances, the height of the top cover can be made into a proper volume according to the experiment requirement, and meanwhile, the device can be used for the isotopic labeling of plants with different appearances 13 CO 2 Real-time monitoring for timely adjustment 13 CO 2 And (4) concentration.
Compare traditional soil destructive sampling and gather root secretion, this device can realize a large amount of effective collections of the root secretion of normal position in the heavy metal contaminated soil and the collection of rhizospheric microorganism of perhaps the same space simultaneously, is same system with DNA-SIP simultaneously, reduces the error of mark experiment. The collection of the root secretion of this device concentrates on near root system, and not to the soil of whole end lower part, has improved collection efficiency.
Compared with the traditional soil material for destructively sampling or in-situ collecting rhizosphere soil microorganisms, the device is a marker of the same system 13 The in-situ collection of C-root secretion-rhizosphere microorganisms realizes the marker 13 And C, tracing the change of plant metabolic pathways, root exudates and rhizosphere microorganisms caused by heavy metal stress, so that the accuracy of the marking result is improved. Meanwhile, the method can also be used for marking tests of other plant growth conditions.
In addition, can also put back in the heavy metal contaminated soil in situ after gathering the root exudates and finishing, painless, convenient, the follow-up proof test of effectual completion root exudates.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
Fig. 1 is a schematic view of the overall structure of the present invention.
In the figures, the various reference numbers are:
1-an air inlet; 2-CO 2 A real-time concentration display; 3-top cover; 4-a vent; 5-plant growing mouth; 6-a collection chamber; 7-a barrier net; 8-silica gel plug; 9-rhizosphere net; 10-root box; 11-gas outlet; 12-a stand leg; 13-a bollard; 14-root box roof.
Detailed Description
In order to more clearly illustrate the present invention, the present invention will be described in further detail below with reference to examples and the accompanying drawings. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
In the description of the present invention, it should be noted that the terms "inside", "outside", "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, and integral connections; can be mechanically or electrically connected; they may be directly connected, indirectly connected through intervening media, or may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The embodiment is as follows:
the device for marking in-situ collection of root exudates and rhizosphere soil as shown in figure 1 comprises a top cover 3 for simulating the ground and a root box 10 for simulating the underground, wherein the top cover 3 is buckled above the root box 10, and the root box10 are filled with soil. The bottom of the root box 10 is provided with support legs 12 to leave a gap with the ground at the bottom of the root box 10. To enable real-time viewing of the inside of the dome 13 CO 2 Concentration, the inside of the top cover 3 is provided with CO 2 Real-time concentration display 2, CO 2 The real-time concentration display 2 is arranged on the top of the top cover 3.
The top cover 3 is a closed transparent space for the ground above the plants 13 CO 2 And (4) marking. The top cover 3 is provided with an air inlet 1 and an air outlet 11, the air inlet 1 is an air-receiving adapter, and the air is filled into the top cover 3 from the air inlet 1 13 CO 2 Selecting a corresponding specification interface according to the size of the interface; the air outlet 11 is used for controlling the inside of the top cover 3 13 CO 2 Concentration of when CO 2 Real-time concentration display 2 shows 13 CO 2 When the concentration is too high, the gas outlet 11 may be opened. The air inlet 1 is arranged at the upper part of the top cover 3, and the air outlet 11 is arranged at the lower part of the top cover 3, so that the position of the air inlet 1 is higher than that of the air outlet 11.
The upper end face of the root box 10 is a root box top cover 14, the root box top cover 14 is used for separating the top cover 3 and the root box 10 (on the ground and below the ground) and is used for placing the top cover 3, the root box top cover 14 is provided with a plant growth opening 5 for the test seedling to pass through, and the plant growth opening 5 is positioned in the top cover 3. Root box top cover 14 is used in cooperation with a sealing film to secure the top cover 3 13 CO 2 Will not enter the space inside the root box 10 through the air circulation, ensuring the space inside the root box 10 13 C is metabolized by the plant into root box 10. Root box top cap 14 is for opening the structure to the centre can be opened to carry out the operation and water root box 10, make soil moisture loss slower simultaneously, reduce external disturbance.
The top cover 14 of the root box is also provided with an air vent 4, and the air vent 4 is positioned outside the top cover 3 and used for ensuring the air circulation of the soil in the root box 10. The four vents 4 of this embodiment are distributed on both sides of the top cover 3.
The inside of root case 10 is equipped with a plurality of root system posts 13, and root system post 13 is the cylinder pipe, when transplanting the examination seedling, arranges the root system gently, and the root tip is arranged in the top mouth of pipe of root system post 13, owing to water and the action of gravity, the root system can grow in root system post 13. A rhizosphere net 9 is arranged outside the root system column 13, the rhizosphere net 9 is a plane formed by fixing two layers of 100-mesh nylon nets through an acrylic rod, and the plane is obliquely arranged at an angle of 45 degrees. Due to the geotropism of the root system, the root system can grow along the rhizosphere net 9, thin soil is covered, and the rhizosphere soil and the plant root system can be collected by slightly picking up the root system.
The below of root case 10 still is equipped with collects chamber 6, collects the below that chamber 6 is located root system post 13, is equipped with the through-hole on the root case 10 to make and collect chamber 6 and root system post 13 intercommunication, simultaneously, the last mouth of pipe department of collecting chamber 6 is equipped with separation net 7, and separation net 7 adopts 100 meshes nylon wire, and the soil of separation root case 10 drops for separate soil and collect chamber 6. The root exudates are collected in the collecting cavity 6 by infiltration with the liquid. The bottom of the collection cavity 6 is provided with a silica gel plug 8 to prevent the liquid in the collection cavity 6 from flowing away.
After the test seedlings are transplanted into the root box 10, the plants utilize the photosynthesis substrate in the top cover 3 13 CO 2 The microorganisms in the soil near the root system utilize the root secretions, which are then also taken up by the microorganisms via various metabolic pathways to the root secretions 13 And C, marking. Soil solution infiltrates to and collects chamber 6 down, and the root system secretion is collected to external negative pressure collecting pipe, and the layer is collected to rhizosphere soil in the root box simultaneously, collects root system and rhizosphere soil microorganism material.
First, the roots of the seedlings are arranged so that the main root system grows at a predetermined position (root system column 13 and rhizosphere net 9). Then, burying the soil in the whole root box 10, watering to keep the soil moisture; and then, after the seedlings grow stably, covering the top cover 14 of the root box, sealing the top cover interface and the plant growing opening by using a sealing film, fastening the top cover 3, and sealing the contact opening.
Filling the top cover 3 in corresponding time periods according to test requirements 13 CO 2 The removable top cover 3 is marked end; watering the soil one day before collecting root exudates to keep the maximum field water capacity, watering a small amount of water later, infiltrating the liquid containing the root exudates in the root column 13 into the collecting cavity 6, extracting the liquid through an external negative pressure pipe, and collecting about 10ML of liquid at night; the rhizosphere soil is in the rhizosphere net 9, the root system is picked, the rhizosphere soil is swept lightly or the rhizosphere soil on the surface of the root system is collected by a rhizosphere soil collecting tube provided by a sequencing companyThe amount of rhizosphere soil required for extraction of microorganisms is small and the disturbance of this operation is very slight. In the whole process, root case 10 outer wall is with the light-resistant of tinfoil parcel, simultaneously, in order to facilitate observing the inside condition, the overhead guard 3 adopts transparent ya keli material with root case 10, and root system post 13 also can adopt transparent ya keli material with collection chamber 6 to observe the growth condition of root system.
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all the embodiments of the present invention are not exhaustive, and all the obvious variations or modifications which are introduced in the technical scheme of the present invention are within the scope of the present invention.
Claims (10)
1. A device for collecting root exudates and rhizosphere soil in situ by marking is characterized by comprising a top cover used for simulating the ground and a root box used for simulating the underground, wherein the root box is filled with soil, and the top cover is buckled above the root box; the top cover is a closed transparent space, an air inlet and an air outlet are arranged on the top cover, and the air inlet is used for filling the top cover 13 CO 2 The air outlet is used for controlling the inside of the top cover 13 CO 2 Concentration; a plurality of root system columns for placing roots of test seedlings are arranged inside the root box, the root system columns are cylindrical tubes, and root tips are arranged at pipe orifices above the root system columns; the below of root case still is equipped with the collection chamber that is used for collecting the root system secretion, it is located the below of root system post to collect the chamber.
2. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in claim 1, wherein the air inlet is disposed at an upper portion of the top cover, the air outlet is disposed at a lower portion of the top cover, and the air inlet is higher than the air outlet.
3. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in claim 1, wherein the upper end surface of the root box is a top cover of the root box, the top cover of the root box separates the top cover and the root box and places the top cover, a plant growth opening for a test seedling to pass through is arranged on the top cover of the root box, and the plant growth opening is positioned in the top cover.
4. The method of claim 3 13 CO 2 Device for marking in-situ collection of root exudates and rhizosphere soil, and is characterized in that a sealing film is arranged at the top cover of a root box to ensure that the top cover is covered 13 CO 2 Can not enter the root box through the gas circulation.
5. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in claim 3, wherein the top cover of the root box is a split structure for operating and watering the root box.
6. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in claim 3, wherein a vent hole for ensuring air circulation of soil in the root box is further formed in the top cover of the root box, and the vent hole is located outside the top cover.
7. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in claim 1, wherein a rhizosphere net for root system climbing growth is further provided, the rhizosphere net is a plane formed by fixing two layers of nylon nets through an acrylic rod, and the rhizosphere net is inclined at 45 degrees.
8. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in claim 1, wherein a blocking net for blocking soil in the root box from falling is arranged at an upper pipe orifice of the collection cavity, and the blocking net is a 100-mesh nylon net.
9. The method of claim 1, wherein the method comprises marking the collection of root exudates and rhizosphere soil in situThe device is characterized in that the inside of the top cover is provided with CO 2 Real-time concentration display of said CO 2 The real-time concentration display is arranged at the top of the top cover.
10. The device for marking in-situ collection of root exudates and rhizosphere soil as claimed in any one of claims 1 to 9, wherein the root box and the top cover are made of transparent acrylic material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210717789.7A CN115219656A (en) | 2022-06-23 | 2022-06-23 | Device for marking in-situ collection of root exudates and rhizosphere soil |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210717789.7A CN115219656A (en) | 2022-06-23 | 2022-06-23 | Device for marking in-situ collection of root exudates and rhizosphere soil |
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| CN115219656A true CN115219656A (en) | 2022-10-21 |
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2022
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| CN102589923A (en) * | 2012-02-10 | 2012-07-18 | 浙江大学 | Plant 13CO2 mark cultivation-root exudate collection integration device |
| CN203658101U (en) * | 2014-01-14 | 2014-06-18 | 西华师范大学 | Device for collecting secretions at root system of seedling and measuring ratio of secretions in photosynthate |
| CN209803097U (en) * | 2019-04-12 | 2019-12-17 | 海南大学 | Root secretion is collected and is allelopathic effect viewing device |
| CN209961502U (en) * | 2019-05-09 | 2020-01-17 | 浙江农林大学暨阳学院 | Plant root system secretion collection device |
| CN112945651A (en) * | 2021-04-06 | 2021-06-11 | 四川农业大学 | Water and soil sharing13CO2Marker culture-root secretion collecting device and method thereof |
| CN216645958U (en) * | 2022-01-06 | 2022-05-31 | 云南省烟草农业科学研究院 | Convenient device capable of collecting cured tobacco root secretion in situ |
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