CN115212607A

CN115212607A - Chromatographic column

Info

Publication number: CN115212607A
Application number: CN202210795248.6A
Authority: CN
Inventors: 张圣麟; 丁小军
Original assignee: Beijing Taishengyuan Biotechnology Co ltd
Current assignee: Beijing Taishengyuan Biotechnology Co ltd
Priority date: 2022-07-08
Filing date: 2022-07-08
Publication date: 2022-10-21

Abstract

The utility model discloses a chromatographic column, be applied to in the liquid chromatogram field, in order to solve the chromatographic column internal flow and form the turbulent flow problem, this patent is through changing chromatographic column and fluid pipeline interface end department structure, retrains and reduces fluid turbulent flow, improves the chromatographic separation effect.

Description

Chromatographic column

Technical Field

The invention relates to the technical field of chromatography, in particular to a brand-new fluid constraint structure of a chromatographic column. The chromatographic column structure can avoid or reduce fluid retention or turbulence at two ends of a flow path of the chromatographic column, and improve the chromatographic separation efficiency.

Background

Chromatography is a widely used technique for separation, analysis and sample preparation. The typical chromatographic column bed is mostly in a cylindrical shape, the fluid diameter of the pipeline at the inlet end and the outlet end of the chromatographic column is far smaller than that of the chromatographic column bed, when fluid (mobile phase or sample) enters or exits the chromatographic column, due to the fact that the cross section area of the fluid is suddenly changed, the fluid can form a cone-like gradually-changed fluid shape at the two ends of the chromatographic column, and the cone-like fluid can form fluid retention or turbulence at the two ends of a traditional chromatographic column to influence the chromatographic separation efficiency.

Disclosure of Invention

The invention provides a novel chromatographic column fluid constraint structure which can avoid or reduce fluid retention or turbulence formed by abrupt change of the cross section area of fluid, thereby improving the chromatographic separation effect. The technical scheme adopted by the invention is as follows: the shape of the fluid is constrained by the specially designed geometric shapes, so that the fluid retention or turbulence forming areas at two ends of the traditional chromatographic column are reduced or eliminated, the retention of the sample at two ends of the chromatographic column is avoided or reduced, and the chromatographic separation efficiency is improved. Can effectively avoid the fluid retention or the resolution reduced by disorder caused by the traditional chromatographic column.

Drawings

FIG. 1 is a schematic cross-sectional view of a typical constrained geometry fluid shape as described in this patent

FIG. 2 is a schematic diagram showing a cross-hatched area of an inlet end fluid retention and turbulence area of a conventional flat-head chromatographic column

FIG. 3 is a schematic diagram showing the fluid retention and turbulent flow region at the outlet end of a conventional flat-head chromatographic column in the shaded area

FIG. 4 is a schematic view of a complete cross-section of a cambered constrained-shape fluid according to the present patent

Detailed Description

Fig. 1 is a schematic cross-sectional view of a typical constrained shape fluid shape as described in this patent, wherein: the device comprises a chromatographic column, a front mobile phase pipeline 1, a front end port 2, a main body part 3, a tail end port 4 and a rear mobile phase pipeline 5.

FIG. 2 is a schematic diagram of the inlet end fluid retention and turbulence zone of a conventional flat-head chromatography column in the shaded area; the fluid can form a fluid stagnation area at the edges of the flat heads 8 and 9; a column shape 10 changes the fluid flow direction, creating a vertical downward flow 11;

FIG. 3 is a schematic diagram of the fluid retention and turbulence zone at the outlet end of a conventional flat-head chromatography column in the shaded area; the fluid will form a stagnant zone of fluid at the edge of the flat head 14; when flowing downwards, the fluid 12 gently converges along the inner wall 13 of the column towards the outlet 15.

The fluid restriction shape can be a gradual change type hollow structure formed by rotating around a straight line through a straight line, a curve, a straight line combination, a curve combination and a combination of the straight line and the curve, and a typical specific implementation structure has the following modes: 1. the hyperbola is connected with the parabola and then rotates around a straight line to form a hollow structure; 2. a hyperbola rotates around a straight line; 2. a conical shape; 3. a hemisphere shape; 4. a combination of a conical shape and a hemispherical shape.

The inlet and outlet ends of the column, either alone or simultaneously, may be used to achieve this goal, either alone or in combination.

The present invention is not limited to the above embodiments, and other structural designs obtained by using a structure similar to the present invention to restrict the gradual change of the shape of the chromatographic column fluid are within the protection scope of the present invention.

Claims

1. A chromatographic column is provided, the connection end of the chromatographic column and a mobile phase pipeline is in a gradual change type fluid restriction shape, the abrupt change of the fluid section is avoided, and fluid stagnation or turbulence is formed at the port.

2. The chromatography column according to claim 1, wherein the tapered confinement shape is connected to a cylindrical space.

3. The cylindrical space of claim 2 may be connected at one or both ends to the tapered fluid confinement shape of claim 1.

4. The gradually changing space of claim 1, which is formed by linearly rotating around a central axis by a straight line, a curved line, a combination of a straight line and a straight line, a combination of a curved line and a curved line, or a combination of a straight line and a curved line.

5. The tapered space of claim 1 is not limited to the one described in claim 4, and may be a space shape similar to the one described in claim 4.

6. The spatial structure according to claim 1, claim 2, claim 3, claim 4 or claim 5 may be partially or completely filled with a chromatographic separation material.

7. The gradual change type space of claim 1, or the joint of the gradual change type space and the mobile phase pipeline, a sieve plate can be added or a sieve plate type structure can be arranged in the gradual change type space, so as to limit the movement of the chromatographic packing.