BR9902312A - Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group - Google Patents
Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes groupInfo
- Publication number
- BR9902312A BR9902312A BR9902312-1A BR9902312A BR9902312A BR 9902312 A BR9902312 A BR 9902312A BR 9902312 A BR9902312 A BR 9902312A BR 9902312 A BR9902312 A BR 9902312A
- Authority
- BR
- Brazil
- Prior art keywords
- quantification
- detection
- mycotoxins
- chromatographic column
- trichothecenes
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
<B>"COLUNA CROMATOGRáFICA PARA PURIFICAçãO DE EXTRATOS VEGETAIS E MéTODO PARA DETECçãO E QUANTIFICAçãO DE MICOTOXINAS DO GRUPO TRICOTECENOS"<D>. Coluna cromatográfica para purificar extratos orgânicos de grãos e ração a serem utilizados na detecção e quantificação de micotoxinas do grupo tricotecenos como,. por exemplo, deoxynivalenol (DON), 3-acetil deoxynivalenol (3 Ac DON), toxina T-2 (T-2) e diacetoxyscirpenol (DAS), assim como método para detecção e quantificação destas micotoxinas. A coluna cromatográfica é constituída por camadas sucessivas e compactas de lã de vidro (2), 3,8 g de sulfato de sódio anidro (3), 0,5 g de terra diatomácea purificada calcinada (4) e 3,7 g de mistura composta por carvão, alumina e terra diatomácea purificada calcinada (5) na proporção de 7:5:3(p/p/p). O método de detecção e quantificação, aplicável a extratos purificados através da coluna, envolve a separação dos tricotecenos por cromatografia em camada delgada, detecção e quantificação por análise visual e análise densitométrica.<B> "CHROMATOGRAPHIC COLUMN FOR PURIFICATION OF VEGETABLE EXTRACTS AND METHOD FOR DETECTION AND QUANTIFICATION OF MYCOTOXINS IN THE TRICOTECENES GROUP" <D>. Chromatographic column to purify organic extracts of grains and feed to be used in the detection and quantification of mycotoxins of the trichothecenes group such as ,. for example, deoxynivalenol (DON), 3-acetyl deoxynivalenol (3 Ac DON), toxin T-2 (T-2) and diacetoxyscirpenol (DAS), as well as a method for detecting and quantifying these mycotoxins. The chromatographic column consists of successive and compact layers of glass wool (2), 3.8 g of anhydrous sodium sulfate (3), 0.5 g of calcined purified diatomaceous earth (4) and 3.7 g of mixture composed of charcoal, alumina and calcined purified diatomaceous earth (5) in the proportion of 7: 5: 3 (w / w / w). The detection and quantification method, applicable to extracts purified through the column, involves the separation of trichothecenes by thin layer chromatography, detection and quantification by visual analysis and densitometric analysis.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9902312-1A BR9902312A (en) | 1999-03-26 | 1999-03-26 | Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9902312-1A BR9902312A (en) | 1999-03-26 | 1999-03-26 | Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9902312A true BR9902312A (en) | 2000-12-12 |
Family
ID=4072642
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9902312-1A BR9902312A (en) | 1999-03-26 | 1999-03-26 | Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group |
Country Status (1)
Country | Link |
---|---|
BR (1) | BR9902312A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101226173B (en) * | 2007-01-16 | 2011-06-08 | 复旦大学 | Method for measuring DON and oxynivalenol in food |
CN115212607A (en) * | 2022-07-08 | 2022-10-21 | 北京太生元生物科技有限公司 | Chromatographic column |
-
1999
- 1999-03-26 BR BR9902312-1A patent/BR9902312A/en not_active IP Right Cessation
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101226173B (en) * | 2007-01-16 | 2011-06-08 | 复旦大学 | Method for measuring DON and oxynivalenol in food |
CN115212607A (en) * | 2022-07-08 | 2022-10-21 | 北京太生元生物科技有限公司 | Chromatographic column |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Schoeman et al. | The impact of a Wastewater Treatment Works in Southern Gauteng, South Africa on efavirenz and nevirapine discharges into the aquatic environment | |
Mohapatra et al. | Occurrence and fate of pharmaceuticals in WWTPs in India and comparison with a similar study in the United States | |
Lambert et al. | Quantitation of the microcystin hepatotoxins in water at environmentally relevant concentrations with the protein phosphatase bioassay | |
Liang et al. | The distribution and partitioning of common antibiotics in water and sediment of the Pearl River Estuary, South China | |
Zhang et al. | Cycling of PCBs and HCB in the surface ocean-lower atmosphere of the open Pacific | |
CA2188161A1 (en) | Intermittent electrolytic packed bed suppressor regeneration for ion chromatography | |
Jiang et al. | Polychlorinated organic compounds (PCOCs) in waters, suspended solids and sediments of the Yangtse River | |
NO980729L (en) | Process for reducing the acid content in crude oil | |
NO912726D0 (en) | SELECTIVE AURAL EXTRACTION OF ORGANIC COMPOUNDS WITH CONSUMPTION COLLECTION BY MEMBRANE SEPARATION. | |
ATE200293T1 (en) | METHOD FOR THE CHROMATOGRAPHIC PURIFICATION AND SEPARATION OF NUCLEIC ACID MIXTURES | |
FR2762834B1 (en) | PROCESS AND PLANT FOR THE TREATMENT OF WASTEWATER AND THEIR APPLICATION TO WINE EFFLUENTS | |
CY1108150T1 (en) | PROCEDURE FOR INFECTED WATER TREATMENT PROCESSING USED WITH NON-POLISH ZEOLITHS WITH DIFFERENT CHARACTERISTICS | |
AU2003238296A8 (en) | Purification of n,n-dimethylacetamide | |
Saleh et al. | Study of Citrus taitensis and radical scavenger activity of the flavonoids isolated | |
AR032957A1 (en) | METHODS FOR IDENTIFYING COMPOUNDS TO REGULATE MASS OR MUSCLE FUNCTION USING RECEIVERS OF CORTICOTROPINE RELEASING FACTOR | |
BR9902312A (en) | Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group | |
Omari et al. | Hydrocarbon removal in an experimental gravel bed constructed wetland | |
FR2818637B1 (en) | PROCESS FOR THE TREATMENT OF C4 HYDROCARBONS COMPRISING BUTADIENE AND ACETYLENIC COMPOUNDS COMPRISING STAGES OF DISTILLATION AND SELECTIVE HYDROGENATION | |
Srikrishna et al. | A simple and convenient one step method for the reductive deoxygenation of aryl ketones to hydrocarbons | |
Quintanilla-López et al. | Comparative study of clean-up and fractionation methods for the determination of organochlorine pesticides in lipids by gas chromatography | |
Goto et al. | Application of High-Performance Liquid Chromatography to the Analysis of Aflatoxins in Foods and Feeds. Prepartation of Sample and Column | |
Zipser et al. | Assay methods for 6-keto-prostaglandin F1α in human urine: Comparison of chromatographic techniques with radioimmunoassay and gas chromatography—negative-ion chemical-ionization mass spectrometry | |
Leone et al. | Ibuprofen adsorption on organo-zeolite: experimental and modelling analysis | |
GB0011107D0 (en) | System and method for detecting computer components by cooperation of two computer hosts | |
FR2833194B1 (en) | DEVICE AND METHOD FOR TREATING MINERAL MATERIALS SUCH AS LAND OR RECOVERY MATERIAL |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application fees: dismissal - article 86 of industrial property law |
Free format text: REFERENTE AO NAO CUMPRIMENTO DA EXIGENCIA PUBLICADA NA RPI 1891 DE 03/04/2007. |
|
B08K | Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87) |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1914 DE 11/09/2007. |