BR9902312A - Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group - Google Patents

Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group

Info

Publication number
BR9902312A
BR9902312A BR9902312-1A BR9902312A BR9902312A BR 9902312 A BR9902312 A BR 9902312A BR 9902312 A BR9902312 A BR 9902312A BR 9902312 A BR9902312 A BR 9902312A
Authority
BR
Brazil
Prior art keywords
quantification
detection
mycotoxins
chromatographic column
trichothecenes
Prior art date
Application number
BR9902312-1A
Other languages
Portuguese (pt)
Inventor
Eliene Alves Dos Santos
Eugenia Azevedo Vargas
Original Assignee
Eliene Alves Dos Santos
Eugenia Azevedo Vargas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eliene Alves Dos Santos, Eugenia Azevedo Vargas filed Critical Eliene Alves Dos Santos
Priority to BR9902312-1A priority Critical patent/BR9902312A/en
Publication of BR9902312A publication Critical patent/BR9902312A/en

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)

Abstract

<B>"COLUNA CROMATOGRáFICA PARA PURIFICAçãO DE EXTRATOS VEGETAIS E MéTODO PARA DETECçãO E QUANTIFICAçãO DE MICOTOXINAS DO GRUPO TRICOTECENOS"<D>. Coluna cromatográfica para purificar extratos orgânicos de grãos e ração a serem utilizados na detecção e quantificação de micotoxinas do grupo tricotecenos como,. por exemplo, deoxynivalenol (DON), 3-acetil deoxynivalenol (3 Ac DON), toxina T-2 (T-2) e diacetoxyscirpenol (DAS), assim como método para detecção e quantificação destas micotoxinas. A coluna cromatográfica é constituída por camadas sucessivas e compactas de lã de vidro (2), 3,8 g de sulfato de sódio anidro (3), 0,5 g de terra diatomácea purificada calcinada (4) e 3,7 g de mistura composta por carvão, alumina e terra diatomácea purificada calcinada (5) na proporção de 7:5:3(p/p/p). O método de detecção e quantificação, aplicável a extratos purificados através da coluna, envolve a separação dos tricotecenos por cromatografia em camada delgada, detecção e quantificação por análise visual e análise densitométrica.<B> "CHROMATOGRAPHIC COLUMN FOR PURIFICATION OF VEGETABLE EXTRACTS AND METHOD FOR DETECTION AND QUANTIFICATION OF MYCOTOXINS IN THE TRICOTECENES GROUP" <D>. Chromatographic column to purify organic extracts of grains and feed to be used in the detection and quantification of mycotoxins of the trichothecenes group such as ,. for example, deoxynivalenol (DON), 3-acetyl deoxynivalenol (3 Ac DON), toxin T-2 (T-2) and diacetoxyscirpenol (DAS), as well as a method for detecting and quantifying these mycotoxins. The chromatographic column consists of successive and compact layers of glass wool (2), 3.8 g of anhydrous sodium sulfate (3), 0.5 g of calcined purified diatomaceous earth (4) and 3.7 g of mixture composed of charcoal, alumina and calcined purified diatomaceous earth (5) in the proportion of 7: 5: 3 (w / w / w). The detection and quantification method, applicable to extracts purified through the column, involves the separation of trichothecenes by thin layer chromatography, detection and quantification by visual analysis and densitometric analysis.

BR9902312-1A 1999-03-26 1999-03-26 Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group BR9902312A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR9902312-1A BR9902312A (en) 1999-03-26 1999-03-26 Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR9902312-1A BR9902312A (en) 1999-03-26 1999-03-26 Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group

Publications (1)

Publication Number Publication Date
BR9902312A true BR9902312A (en) 2000-12-12

Family

ID=4072642

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9902312-1A BR9902312A (en) 1999-03-26 1999-03-26 Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group

Country Status (1)

Country Link
BR (1) BR9902312A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101226173B (en) * 2007-01-16 2011-06-08 复旦大学 Method for measuring DON and oxynivalenol in food
CN115212607A (en) * 2022-07-08 2022-10-21 北京太生元生物科技有限公司 Chromatographic column

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101226173B (en) * 2007-01-16 2011-06-08 复旦大学 Method for measuring DON and oxynivalenol in food
CN115212607A (en) * 2022-07-08 2022-10-21 北京太生元生物科技有限公司 Chromatographic column

Similar Documents

Publication Publication Date Title
Schoeman et al. The impact of a Wastewater Treatment Works in Southern Gauteng, South Africa on efavirenz and nevirapine discharges into the aquatic environment
Mohapatra et al. Occurrence and fate of pharmaceuticals in WWTPs in India and comparison with a similar study in the United States
Lambert et al. Quantitation of the microcystin hepatotoxins in water at environmentally relevant concentrations with the protein phosphatase bioassay
Liang et al. The distribution and partitioning of common antibiotics in water and sediment of the Pearl River Estuary, South China
Zhang et al. Cycling of PCBs and HCB in the surface ocean-lower atmosphere of the open Pacific
CA2188161A1 (en) Intermittent electrolytic packed bed suppressor regeneration for ion chromatography
Jiang et al. Polychlorinated organic compounds (PCOCs) in waters, suspended solids and sediments of the Yangtse River
NO980729L (en) Process for reducing the acid content in crude oil
NO912726D0 (en) SELECTIVE AURAL EXTRACTION OF ORGANIC COMPOUNDS WITH CONSUMPTION COLLECTION BY MEMBRANE SEPARATION.
ATE200293T1 (en) METHOD FOR THE CHROMATOGRAPHIC PURIFICATION AND SEPARATION OF NUCLEIC ACID MIXTURES
FR2762834B1 (en) PROCESS AND PLANT FOR THE TREATMENT OF WASTEWATER AND THEIR APPLICATION TO WINE EFFLUENTS
CY1108150T1 (en) PROCEDURE FOR INFECTED WATER TREATMENT PROCESSING USED WITH NON-POLISH ZEOLITHS WITH DIFFERENT CHARACTERISTICS
AU2003238296A8 (en) Purification of n,n-dimethylacetamide
Saleh et al. Study of Citrus taitensis and radical scavenger activity of the flavonoids isolated
AR032957A1 (en) METHODS FOR IDENTIFYING COMPOUNDS TO REGULATE MASS OR MUSCLE FUNCTION USING RECEIVERS OF CORTICOTROPINE RELEASING FACTOR
BR9902312A (en) Chromatographic column for purification of plant extracts and method for the detection and quantification of mycotoxins of the trichothecenes group
Omari et al. Hydrocarbon removal in an experimental gravel bed constructed wetland
FR2818637B1 (en) PROCESS FOR THE TREATMENT OF C4 HYDROCARBONS COMPRISING BUTADIENE AND ACETYLENIC COMPOUNDS COMPRISING STAGES OF DISTILLATION AND SELECTIVE HYDROGENATION
Srikrishna et al. A simple and convenient one step method for the reductive deoxygenation of aryl ketones to hydrocarbons
Quintanilla-López et al. Comparative study of clean-up and fractionation methods for the determination of organochlorine pesticides in lipids by gas chromatography
Goto et al. Application of High-Performance Liquid Chromatography to the Analysis of Aflatoxins in Foods and Feeds. Prepartation of Sample and Column
Zipser et al. Assay methods for 6-keto-prostaglandin F1α in human urine: Comparison of chromatographic techniques with radioimmunoassay and gas chromatography—negative-ion chemical-ionization mass spectrometry
Leone et al. Ibuprofen adsorption on organo-zeolite: experimental and modelling analysis
GB0011107D0 (en) System and method for detecting computer components by cooperation of two computer hosts
FR2833194B1 (en) DEVICE AND METHOD FOR TREATING MINERAL MATERIALS SUCH AS LAND OR RECOVERY MATERIAL

Legal Events

Date Code Title Description
B08F Application fees: dismissal - article 86 of industrial property law

Free format text: REFERENTE AO NAO CUMPRIMENTO DA EXIGENCIA PUBLICADA NA RPI 1891 DE 03/04/2007.

B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1914 DE 11/09/2007.