CN115211378A - Animal experiment inhalable particle simulation case - Google Patents
Animal experiment inhalable particle simulation case Download PDFInfo
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- CN115211378A CN115211378A CN202110417355.0A CN202110417355A CN115211378A CN 115211378 A CN115211378 A CN 115211378A CN 202110417355 A CN202110417355 A CN 202110417355A CN 115211378 A CN115211378 A CN 115211378A
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- 238000004088 simulation Methods 0.000 title claims abstract description 25
- 241001465754 Metazoa Species 0.000 title claims abstract description 23
- 238000002474 experimental method Methods 0.000 title claims abstract description 19
- 239000002245 particle Substances 0.000 title claims abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 18
- 239000001301 oxygen Substances 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 238000012544 monitoring process Methods 0.000 claims abstract description 6
- 239000010410 layer Substances 0.000 claims description 63
- 239000000779 smoke Substances 0.000 claims description 36
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 18
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 9
- 238000005192 partition Methods 0.000 claims description 9
- 238000005057 refrigeration Methods 0.000 claims description 8
- 239000001569 carbon dioxide Substances 0.000 claims description 7
- 239000007789 gas Substances 0.000 claims description 6
- 239000011229 interlayer Substances 0.000 claims description 5
- 239000012780 transparent material Substances 0.000 claims description 5
- 238000009825 accumulation Methods 0.000 claims description 2
- 230000000384 rearing effect Effects 0.000 claims 2
- 230000000630 rising effect Effects 0.000 claims 1
- 230000007918 pathogenicity Effects 0.000 abstract description 3
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 230000003434 inspiratory effect Effects 0.000 abstract 1
- 239000003344 environmental pollutant Substances 0.000 description 4
- 238000010171 animal model Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- 239000013618 particulate matter Substances 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011362 coarse particle Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/0047—Air-conditioning, e.g. ventilation, of animal housings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/0047—Air-conditioning, e.g. ventilation, of animal housings
- A01K1/0064—Construction of air inlets or outlets in walls
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/01—Removal of dung or urine, e.g. from stables
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Housing For Livestock And Birds (AREA)
Abstract
The invention belongs to the technical field of experimental equipment, and relates to an inhalable particle simulation box for animal experiments. This simulation case PM1.0 in can real-time supervision simulation cabin, PM2.5, PM10 concentration, the simultaneous monitoring controls illumination, the temperature, oxygen concentration, external influence factors such as CO2 concentration, the device can carry out the experiment of the majority of multiunit simultaneously, guarantee the uniformity of experimental condition, reduce simultaneously experiment cost and cycle and avoided experimenter direct contact environmental pollution, can effectually establish the animal research model who is close to reality atmospheric pollution pathogenicity through inspiratory mode.
Description
Technical Field
The invention belongs to the technical field of experimental equipment, relates to the field of environment simulation boxes, and particularly relates to an inhalable particle simulation box for an animal experiment.
Background
Atmospheric pollution has been reported to be a public health problem seriously harming health in our country. The atmospheric pollutants are complex in composition and can be divided into two main categories, namely particulate pollutants (mainly including inhalable particulate matters, fine particulate matters and the like) and gaseous pollutants (mainly including sulfur dioxide, carbon monoxide, nitrogen oxides, ozone and the like) according to forms, wherein the atmospheric Particulate Matters (PM) are a general term for various solid particulate matters and liquid particulate matters in the atmosphere. According to the environmental regulation document of the United states environmental protection agency, coarse particles or absorbable particles (PM 10) with aerodynamic diameter size less than or equal to 10 μm and fine particles (PM 2.5) with aerodynamic diameter size less than or equal to 2.5 μm are called. The 2010 global disease burden data shows that PM causes about 123 million deaths in China, and is associated with various systemic diseases of the whole body. Among them, PM2.5 is smaller in size, lighter in weight, easier to enter the blood system, and may be more closely related to diseases. However, at present, there is no systematic research evidence, and an effective animal research model is lacked. At present, the establishment of the existing PM and PM2.5 animal models basically depends on an invasive operation method of airway instillation, repeated anesthesia and repeated invasion increase airway inflammatory reaction, and reduce the accuracy of the animal models.
Based on the current situation of the prior art, the inventor of the application plans to provide an animal experiment inhalable particle environment simulation case, and when this simulation case monitors the inhaled particle concentration in the storehouse, steerable illumination, temperature, oxygen concentration, CO 2 Concentration and other external influence factors, the application aims to establish an animal model closer to a real state in an inhalation mode.
Disclosure of Invention
The purpose of the invention is based on the prior artThe current state of the technology provides an animal experiment inhalable particle environment simulation box, and when the simulation box monitors the concentration of the inhalable particles in a bin, the simulation box can control illumination, temperature, oxygen concentration and CO 2 Concentration and the like.
The technical scheme adopted by the invention for solving the technical problems is as follows: the environment simulation box for inhalable particles in animal experiments comprises an equipment box body (1), an LED illuminating lamp (2), a controller display screen (3), a circulating air pipe (4), a parameter monitoring sensor (5), a smoke exhaust check valve (6), a pollution discharge floor drain (7), an equipment foot pad (8), a smoke inlet (9), an equipment cabin door (10) and a refrigeration module (11);
the environment simulation box is divided into an upper layer structure and a lower layer structure, wherein the upper layer structure is a control layer, and the lower layer structure is an animal movable breeding layer; the LED illuminating lamp is arranged on a partition plate in the middle of the equipment, an interlayer in the middle of an equipment box body made of transparent materials can provide illumination for the lower feeding layer through the partition plate, and meanwhile, a pipeline of a circulating air pipeline and a circulating fan are arranged on the partition plate in the middle of the equipment and are used for uniformly mixing gas in the lower feeding layer; the lower surface of a partition plate in the middle of each parameter sensor mounting device is communicated with the lower layer feeding space, the lower layer of the box body is provided with a cabin door, the left side bottom of the lower layer box body is provided with a smoke inlet, the right side bottom of the lower layer box body is provided with a smoke exhaust fan and a check valve, the air inlet surface of the smoke exhaust fan is attached to the wall of the box body, and the check valve is attached to the air outlet surface of the smoke exhaust fan; the controller display screen is embedded in the outer side of the upper layer of the equipment box body, and touch control is facilitated.
The equipment box body is made of transparent materials, and the total volume of the box body is as follows: 60x50x40 (cm), the box adopts seal structure, and no gas leakage exists between upper and lower layer structure.
The smoke inlet and the smoke exhaust check valve are arranged on the opposite side of the equipment, smoke is discharged from the left side of the equipment, smoke is discharged from the right side of the equipment, or smoke is discharged from the right side of the equipment, and smoke is discharged from the left side of the equipment.
The circulating air pipe is arranged on the upper layer of the equipment box body and used for circulating and uniformly mixing the air on the lower layer of the equipment through the circulating air pipe by air outlet on the left side and the right side of the double fans.
The parameter monitoring sensor is provided with an oxygen content sensor, a carbon dioxide content sensor, a temperature and humidity sensor and PM1.0, PM2.5 and PM10 inhalable particle sensors.
And the lower feeding layer of the box body is provided with a refrigerating module for controlling the temperature reduction of the raising layer environment caused by the accumulation of the carbon dioxide content in a closed state.
The equipment power supply equipment is powered by 5V and 12V direct current, the refrigeration module adopts 12V semiconductor refrigeration sheets, a fan is arranged at the cold end of the refrigeration module and is arranged in the lower-layer space of the box body, and a fan and a heat-conducting sheet are adopted at the hot end of the upper layer for heat conduction.
The invention provides an environment simulation box for inhalable particles in animal experiments, which can monitor the concentrations of PM1.0, PM2.5 and PM10 in a simulation cabin in real time, monitor and control external influence factors such as illumination, temperature, oxygen concentration, CO2 concentration and the like, and effectively establish an animal research model close to actual atmospheric pollution pathogenicity in an inhalation mode. The device convenient operation can carry out the experiment of the most quantity of multiunit animal simultaneously, has guaranteed the uniformity of experimental condition, greatly reduces experiment cost and cycle simultaneously, has still avoided experimenter direct contact environmental pollutant in addition, effectively protects operator's health and safety.
The invention is further illustrated with reference to the following figures and examples.
Drawings
Figure 1 is a general block diagram of the apparatus of the invention,
FIG. 2 is a view showing the construction of an upper and lower double-deck box body of the present invention,
figure 3 is a block diagram of the circulating air system of the present invention,
figure 4 is a component mounting profile of the circulating air system of the present invention,
figure 5 is a distribution diagram of the housing components and sensor mounting of the present invention,
the reference numbers in the figures are: the device comprises a device box body (1), an LED illuminating lamp (2), a controller display screen (3), a circulating air pipe (4), a parameter monitoring sensor (5), a smoke exhaust check valve (6), a sewage drainage floor drain (7), a device foot pad (8), a smoke inlet (9), a device cabin door (10), a refrigeration module (11), a device box body middle partition layer (1-1), a display control screen installation position (1-2), a device box body top cover (1-3), a lower-layer box body exhaust port (1-4), a sewage drainage floor drain installation port (1-5), a smoke inlet installation port (1-6), a circulating air inlet fan (4-1), an air inlet fan fixing plate (4-2), an oxygen adding pump (4-3), an oxygen supply pipeline (4-4), an air outlet fan fixing plate (4-5), a circulating air outlet fan (4-6), an oxygen content sensor (5-1), a carbon dioxide content sensor (5-2), a temperature and humidity sensor (5-3), a particulate matter sensor (5-4) and an exhaust fan (6-1).
Detailed Description
The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic views illustrating only the basic structure of the present invention in a schematic manner, and thus show only the constitution related to the present invention.
As shown in fig. 1, an LED illuminating lamp is installed in an upper layer structure of an equipment box body, a middle spacing layer of the equipment box body made of transparent materials plays a good role in light transmission, even if the LED illuminating lamp is installed in the upper layer structure, the LED illuminating lamp can also provide illumination for a lower layer box body, a circulating air pipe is installed on the upper layer of the box body, air inlets and air outlets of double fans of a circulating air pipe are communicated with the lower layer space of the box body, the double fans provide uniform mixing work of lower layer gas of the box body, an oxygen sensor, a carbon dioxide sensor, a temperature and humidity sensor and a particulate matter sensor are installed on the lower surface of the middle spacing layer of the equipment box body and directly contact with the lower layer space of the box body, and various gas parameters in the lower layer space of the box body can be rapidly and accurately measured; the lower layer of the box body is provided with a pollution discharge floor drain for cleaning and discharging waste materials of raised animals, and the left side and the right side of the box body are respectively provided with a smoke inlet and a smoke discharge check valve for filling smoke into the lower layer space of the equipment box body and discharging redundant smoke; the front panel at the lower layer of the box body is provided with a hatch door for placing and taking out the animals required to be tested.
As shown in fig. 2, the whole frame of the equipment box body is provided with a display control screen mounting position for mounting a display control screen on the front panel of the upper layer of the equipment, a middle interlayer of the equipment box body is mounted in the middle of the equipment box body for mounting a sensor, a circulating air pipe and an LED illuminating lamp, and a smoke inlet mounting opening for mounting a smoke pipe is formed on the left panel of the lower layer of the equipment; the lower-layer right side panel of the equipment is provided with a lower-layer box exhaust port, and the lower-layer bottom panel of the equipment is provided with a pollution discharge floor drain mounting port for mounting a pollution discharge floor drain.
A circulating air inlet fan is arranged on an air inlet fan fixing plate on the left side of the circulating air pipe structure shown in figures 3-4, the air inlet fan is arranged in the circulating air pipe, and the left side structure of the circulating air pipe is fixed on a middle interlayer of an equipment box body by using a screw hole on the air inlet fan fixing plate; a circulating air outlet fan is installed on an air outlet fan fixing plate on the right side, the air outlet fan is installed in the circulating air pipe, and a right side structure of the circulating air pipe is fixed on a middle interlayer of the equipment box body through a screw hole in the air outlet fan fixing plate; the left fan sucks the air in the lower layer space of the box body into the circulating air pipe, and the right fan blows the air in the pipeline into the lower layer space of the box body, so that a circulating process is formed; an oxygen supply pipeline is arranged in the middle of the circulating air pipe, one end of the oxygen supply pipeline is connected with an oxygen adding pump, and the other end of the oxygen supply pipeline is connected with the circulating air pipe and is used for providing oxygen under the condition that oxygen generated in the lower layer feeding process of the box body is insufficient.
A smoke inlet shown in fig. 5 is installed on the left side of the equipment box body, an LED illuminating lamp is installed in the box body and close to the left side plate of the box body, a circulating air pipe system is installed in the middle of the box body, an oxygen sensor, a carbon dioxide sensor, a temperature and humidity sensor and a particulate matter sensor are installed in the middle of the circulating air pipe, data of the sensors are all transmitted to a display screen of a controller, general display and regulation are performed by the display screen of the controller, a refrigeration module is installed on the rear portion of the sensor, and a cold end of the refrigeration module is installed in a lower layer space of the box body; an exhaust fan and a smoke exhaust check valve are arranged on the right side panel of the equipment box body to exhaust smoke in the lower layer of the equipment box body.
Experiments prove that the simulation box can monitor the concentrations of PM1.0, PM2.5 and PM10 in the simulation box in real time, and monitor and control external influence factors such as illumination, temperature, oxygen concentration, CO2 concentration and the like at the same time.
The simulation box can effectively establish an animal research model close to actual atmospheric pollution pathogenicity in a suction mode.
Claims (6)
1. An environment simulation box for inhalable particles in animal experiments is characterized by being divided into an upper layer structure and a lower layer structure, wherein the upper layer structure is a control layer, and the lower layer structure is an animal movable feeding layer and comprises an equipment box body (1), an LED illuminating lamp (2), a controller display screen (3), a circulating air pipe (4), a parameter monitoring sensor (5), a smoke exhaust check valve (6), a pollution discharge floor drain (7), an equipment foot pad (8), a smoke inlet (9), an equipment cabin door (10) and a refrigerating module (11);
the LED illuminating lamp is arranged on a partition plate in the middle of the equipment, an interlayer in the middle of an equipment box body made of transparent materials can provide illumination for the lower feeding layer through the partition plate, and meanwhile, a pipeline of a circulating air pipeline and a circulating fan are arranged on the partition plate in the middle of the equipment and are used for uniformly mixing gas in the lower feeding layer; the lower surface of a partition plate in the middle of each parameter sensor mounting device is communicated with the lower layer feeding space, the lower layer of the box body is provided with a cabin door, the left side bottom of the lower layer box body is provided with a smoke inlet, the right side bottom of the lower layer box body is provided with a smoke exhaust fan and a check valve, the air inlet surface of the smoke exhaust fan is attached to the wall of the box body, and the check valve is attached to the air outlet surface of the smoke exhaust fan; the controller display screen is embedded in the outer side of the upper layer of the equipment box body, and touch control is facilitated.
2. The animal experiment inhalable particle environment simulation box of claim 1, wherein the equipment box body is made of transparent material, and the total volume of the box body is as follows: 60x50x40 (cm), the box adopts seal structure, and no gas leakage exists between upper and lower layer structure.
3. The animal experiment inhalable particle environment simulation box of claim 1, wherein the smoke inlet and the smoke exhaust check valve are installed on the opposite side of the equipment, smoke is discharged from the left side of the equipment, smoke is discharged from the right side of the equipment, or smoke is discharged from the right side of the equipment, smoke is discharged from the left side of the equipment.
4. The animal experiment inhalable particle environment simulation box of claim 1, wherein the circulating air pipe is installed on an upper layer of the equipment box body, and air in a lower layer of the equipment is circulated and uniformly mixed through the circulating air pipe by air inlet at the left side and air outlet at the right side of the double fans.
5. The animal experiment inhalable particle environment simulation kit according to claim 1, wherein the parameter monitoring sensor is installed with an oxygen content sensor, a carbon dioxide content sensor, a temperature and humidity sensor, and a PM1.0, PM2.5, PM10 inhalable particle sensor.
6. The animal experiment inhalable particle environment simulation box of claim 1, wherein a refrigeration module is installed on the lower rearing layer of the box body and used for controlling the temperature of the rearing layer environment rising caused by the accumulation of carbon dioxide content in a closed state.
Priority Applications (1)
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CN202110417355.0A CN115211378A (en) | 2021-04-19 | 2021-04-19 | Animal experiment inhalable particle simulation case |
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CN202110417355.0A CN115211378A (en) | 2021-04-19 | 2021-04-19 | Animal experiment inhalable particle simulation case |
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CN115211378A true CN115211378A (en) | 2022-10-21 |
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CN202110417355.0A Pending CN115211378A (en) | 2021-04-19 | 2021-04-19 | Animal experiment inhalable particle simulation case |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN201757749U (en) * | 2010-08-04 | 2011-03-09 | 中国人民解放军第三军医大学 | Simulated sealed cabinet smoke contamination experiment device |
CN112379041A (en) * | 2020-09-10 | 2021-02-19 | 北京麋鹿生态实验中心(北京生物多样性保护研究中心、北京南海子麋鹿苑博物馆) | Experimental system for researching influence of atmospheric particulates on human health |
-
2021
- 2021-04-19 CN CN202110417355.0A patent/CN115211378A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN201757749U (en) * | 2010-08-04 | 2011-03-09 | 中国人民解放军第三军医大学 | Simulated sealed cabinet smoke contamination experiment device |
CN112379041A (en) * | 2020-09-10 | 2021-02-19 | 北京麋鹿生态实验中心(北京生物多样性保护研究中心、北京南海子麋鹿苑博物馆) | Experimental system for researching influence of atmospheric particulates on human health |
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