CN115209915A - 募集dna聚合酶用于模板化编辑 - Google Patents

募集dna聚合酶用于模板化编辑 Download PDF

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Publication number
CN115209915A
CN115209915A CN202180017782.8A CN202180017782A CN115209915A CN 115209915 A CN115209915 A CN 115209915A CN 202180017782 A CN202180017782 A CN 202180017782A CN 115209915 A CN115209915 A CN 115209915A
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CN
China
Prior art keywords
dna
lys
leu
nucleic acid
glu
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Pending
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CN202180017782.8A
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English (en)
Chinese (zh)
Inventor
J·聂
A·哈默尔
S·J·拉维特
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Pairing Plant Service Co ltd
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Pairing Plant Service Co ltd
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Publication of CN115209915A publication Critical patent/CN115209915A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
CN202180017782.8A 2020-01-06 2021-01-06 募集dna聚合酶用于模板化编辑 Pending CN115209915A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062957542P 2020-01-06 2020-01-06
US62/957,542 2020-01-06
PCT/US2021/012283 WO2021141970A1 (en) 2020-01-06 2021-01-06 Recruitment of dna polymerase for templated editing

Publications (1)

Publication Number Publication Date
CN115209915A true CN115209915A (zh) 2022-10-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180017782.8A Pending CN115209915A (zh) 2020-01-06 2021-01-06 募集dna聚合酶用于模板化编辑

Country Status (10)

Country Link
US (2) US12173335B2 (https=)
EP (1) EP4087599A4 (https=)
JP (1) JP7765390B2 (https=)
KR (1) KR20220125304A (https=)
CN (1) CN115209915A (https=)
AU (1) AU2021206639A1 (https=)
CA (1) CA3165756A1 (https=)
IL (1) IL294190A (https=)
MX (1) MX2022008359A (https=)
WO (1) WO2021141970A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2022343271A1 (en) * 2021-09-08 2024-03-28 Flagship Pioneering Innovations Vi, Llc Recruitment in trans of gene editing system components
WO2023133415A1 (en) * 2022-01-04 2023-07-13 Inscripta, Inc. Strategies for direct recruitment of repair templates to crispr nucleases
US11866728B2 (en) 2022-01-21 2024-01-09 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
US20260091137A1 (en) * 2022-09-15 2026-04-02 Enceladus Bio, Inc. Retron directed gene editing

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138176A (zh) * 2015-08-19 2018-06-08 阿克生物公司 使用基于核酸引导的核酸酶的系统捕获核酸
US20190055543A1 (en) * 2016-03-01 2019-02-21 University Of Florida Research Foundation, Incorporated Molecular Cell Diary System
US20190062734A1 (en) * 2016-04-13 2019-02-28 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
WO2019051097A1 (en) * 2017-09-08 2019-03-14 The Regents Of The University Of California RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USING SAME
CN110607355A (zh) * 2019-02-18 2019-12-24 华东理工大学 一种基于Cas9切口酶偶联DNA聚合酶的恒温核酸检测分析方法及试剂盒

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US6627424B1 (en) 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
JP5818688B2 (ja) 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物
EP2786148A4 (en) * 2011-03-31 2015-12-16 Tgr Biosciences Pty Ltd DETECTION OF ANALYTES WITH A SINGLE IMMUNE TEST
WO2014194190A1 (en) 2013-05-30 2014-12-04 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138176A (zh) * 2015-08-19 2018-06-08 阿克生物公司 使用基于核酸引导的核酸酶的系统捕获核酸
US20190055543A1 (en) * 2016-03-01 2019-02-21 University Of Florida Research Foundation, Incorporated Molecular Cell Diary System
US20190062734A1 (en) * 2016-04-13 2019-02-28 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
WO2019051097A1 (en) * 2017-09-08 2019-03-14 The Regents Of The University Of California RNA-GUIDED ENDONUCLEASE FUSION POLYPEPTIDES AND METHODS OF USING SAME
CN110607355A (zh) * 2019-02-18 2019-12-24 华东理工大学 一种基于Cas9切口酶偶联DNA聚合酶的恒温核酸检测分析方法及试剂盒

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANDREW V. ANZALONE等: "Search-and-replace genome editing without double-strand breaks or donor DNA", 《NATURE》, vol. 576, pages 149 - 157, XP055980447, DOI: 10.1038/s41586-019-1711-4 *
GAELEN T HESS等: "Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells", 《NATURE METHODS》, vol. 13, no. 12, pages 1036 - 1045 *
SHAKKED O. HALPERIN等: "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window", 《NATURE》, vol. 560, pages 248 - 252, XP036563463, DOI: 10.1038/s41586-018-0384-8 *
SILVANA KONERMANN等: "Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex", 《NATURE》, vol. 517, no. 7536, pages 583 - 588, XP055206834, DOI: 10.1038/nature14136 *

Also Published As

Publication number Publication date
JP2023510744A (ja) 2023-03-15
MX2022008359A (es) 2022-10-07
EP4087599A1 (en) 2022-11-16
CA3165756A1 (en) 2021-07-15
US20250084390A1 (en) 2025-03-13
IL294190A (en) 2022-08-01
AU2021206639A1 (en) 2022-07-14
US12173335B2 (en) 2024-12-24
US20210207113A1 (en) 2021-07-08
JP7765390B2 (ja) 2025-11-06
KR20220125304A (ko) 2022-09-14
EP4087599A4 (en) 2024-01-24
WO2021141970A1 (en) 2021-07-15

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