CN115202023B - Microscope objective lens with 5 times long working distance - Google Patents
Microscope objective lens with 5 times long working distance Download PDFInfo
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- CN115202023B CN115202023B CN202210907103.0A CN202210907103A CN115202023B CN 115202023 B CN115202023 B CN 115202023B CN 202210907103 A CN202210907103 A CN 202210907103A CN 115202023 B CN115202023 B CN 115202023B
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- 230000003287 optical effect Effects 0.000 claims abstract description 25
- 238000003384 imaging method Methods 0.000 claims abstract description 8
- 239000006185 dispersion Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 11
- 238000001228 spectrum Methods 0.000 description 4
- 230000004075 alteration Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000005304 optical glass Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
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Abstract
The application discloses a microscope objective lens with long working distance, which is configured to be 4-6 times of imaging magnification and long working distance, wherein the objective lens comprises an object plane and an image planeThe first lens group, the second lens group and the third lens group are sequentially arranged upwards along the optical axis, one side of the first lens group facing the image surface is adjacent to one side of the second lens group facing the object surface, and one side of the second lens group facing the image surface is adjacent to one side of the third lens group facing the object surface; the microscope objective satisfies the relationship: wherein f 1 F is the combined focal length of the first lens group 2 And f is the combined focal length of the microscope objective lens. The microscope objective of the application has long working distance and good full-view optical resolution.
Description
Technical Field
The application relates to the field of physical optics, in particular to a microscope objective lens with a 5-fold length working distance.
Background
The microscope is used as an important optical instrument for observing micro objects, has wide application, and along with the continuous expansion of application fields, the requirements on the microscope are higher and higher, and especially the working distance of the microscope objective lens is required to be long, the high resolution is required to be good, and the like. Generally, the working distance of a microscope objective is long, and it is often difficult to obtain good optical resolution of the whole field of view; and the objective lens with better optical resolution is often smaller in working distance and inconvenient to use.
Achieving a microscope objective with good full field optical resolution while achieving longer working distances is a challenge.
The above disclosure of background art is only for aiding in understanding the inventive concept and technical solution of the present application, and it does not necessarily belong to the prior art of the present patent application, nor does it necessarily give technical teaching; the above background should not be used to assess the novelty and creativity of the present application in the event that no clear evidence indicates that such is already disclosed prior to the filing date of the present patent application.
Disclosure of Invention
The application aims to provide a microscope objective lens with 5 times of long working distance and good full-view field optical resolution.
In order to achieve the above purpose, the application adopts the following technical scheme:
a microscope objective configured as an infinity objective having an imaging magnification of 4 to 6 times a working distance, comprising a first lens group, a second lens group, and a third lens group arranged in this order along an optical axis in a direction from an object plane to an image plane, the first lens group having a side facing the image plane disposed adjacent to the second lens group having a side facing the object plane, the second lens group having a side facing the image plane disposed adjacent to the third lens group having a side facing the object plane;
the microscope objective satisfies the relationship:
wherein f 1 F is the combined focal length of the first lens group 2 And f is the combined focal length of the microscope objective lens.
Further, the third lens group comprises a cemented lens consisting of a biconvex lens and a biconcave lens, and the microscope objective lens satisfies the relation:
wherein f 3 Is the combined focal length of the third lens group.
Further, the total number of the cemented lenses and the single lenses of the first lens group, the second lens group and the third lens group is less than or equal to two; and/or the number of the groups of groups,
the optical axis distance between the first lens group and the second lens group is smaller than or equal to 3mm, and the optical axis distance between the second lens group and the third lens group is smaller than or equal to 3mm.
Further, the first lens group includes a cemented lens composed of a negative lens and a biconvex lens, which satisfies the relation: and/or the number of the groups of groups,
N m1 -N p1 >0.12, where N m1 Refractive index of the negative lens of the first lens group, N p1 Refractive index of the biconvex lens of the first lens group; and/or the number of the groups of groups,
V dp1 -V dm1 >12, wherein V dp1 For the Abbe's number, V, of the lenticular lenses of the first lens group dm1 Is the abbe number of the negative lens of the first lens group.
Further, the third lens group includes a cemented lens composed of a biconvex lens and a biconcave lens, which satisfies the relation:
-10<V dm2 -V dp2 <10, wherein V dm2 For the dispersion coefficient, V, of the biconcave lens of the third lens group dp2 Is the abbe number of the lenticular lens of the third lens group.
Further, the second lens group includes a positive single lens that satisfies the relation:wherein R is 1 A mirror surface curvature radius closest to the object surface of the second lens group; and/or the number of the groups of groups,
the mirror surface of the third lens group closest to the image surface is a concave surface, and the relation formula is satisfied:wherein R is 2 The radius of curvature of the mirror surface closest to the image plane of the third lens group.
Further, the second lens group includes a positive single lens that satisfies the relation:
dP g,f >0.005, where dP g,f The relative partial dispersion coefficient of the positive single lens of the second lens group is calculated as follows:
wherein n is g Refractive index of g line, n f Refractive index of F line, n d Refractive index of d line, n c Is the refractive index of the C-line.
Further, the microscope objective satisfies the relationship:
wherein d 0 Is the distance from the object plane to the object plane side mirror plane of the first mirror group.
Further, the microscope objective satisfies the relationship:
0.1< NA <0.2, wherein NA is the object space numerical aperture of the microscope objective.
Further, a ratio value of a distance from an object plane to an object plane side mirror plane of the first lens group to a combined focal length of the microscope objective lens is between 0.85 and 1.25;
the second lens group is a positive single lens, the third lens group is a cemented lens consisting of a biconvex lens and a biconcave lens, and the microscope objective lens satisfies the relation:
wherein R is 1 The radius of curvature of the mirror surface closest to the object plane of the second lens group, R 2 The radius of curvature of the mirror surface closest to the image surface of the third lens group;
wherein f 1 F is the combined focal length of the first lens group 2 F is the combined focal length of the second lens group 3 And f is the combined focal length of the microscope objective lens.
The technical scheme provided by the application has the following beneficial effects:
a. a microscope objective having a longer working distance is provided;
b. the microscope objective exhibits excellent imaging quality at MTF values near the diffraction limit at a representative 0 field, 0.5 field and maximum field.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to the drawings without inventive effort to those skilled in the art.
FIG. 1 is a schematic view of a microscope objective according to an exemplary embodiment of the present application;
fig. 2 is a graph of the transfer function MTF of a microscope objective provided in an exemplary embodiment of the application.
Wherein, the reference numerals include: 101-negative lens, 102-biconvex lens, 201-positive single lens, 301-biconvex lens, 302-biconcave lens.
Detailed Description
In order that those skilled in the art will better understand the present application, a technical solution in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and the claims of the present application and the above figures are used for distinguishing between similar objects and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used may be interchanged where appropriate such that the embodiments of the application described herein may be implemented in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, apparatus, article, or device that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed or inherent to such process, method, article, or device.
In one embodiment of the present application, there is provided a microscope objective configured as an infinity objective having a long working distance of 4 to 6 times of imaging magnification, the microscope objective including a first lens group, a second lens group, and a third lens group arranged in this order along an optical axis in a direction from an object plane to an image plane, a side of the first lens group facing the image plane being disposed adjacent to a side of the second lens group facing the object plane, and a side of the second lens group facing the image plane being disposed adjacent to a side of the third lens group facing the object plane.
In one embodiment of the present application, the distance between the adjacent sets is less than or equal to 10mm, and in another embodiment of the present application, the distance between the optical axes of the first lens group and the second lens group is less than or equal to 3mm, and the distance between the optical axes of the second lens group and the third lens group is less than or equal to 3mm.
The present application is directed to a microscope objective lens with a small number of single lenses, a long working distance and a good resolution, in one embodiment of the present application, referring to fig. 1, the number of single lenses of the first lens group is two (the first lens group may also exist in the form of one cemented lens), the number of single lenses of the second lens group is one, the number of single lenses of the third lens group is two (the third lens group may also exist in the form of one cemented lens), specifically, as shown in fig. 1, the first lens group includes a cemented lens composed of a negative lens 101 and a biconvex lens 102, the second lens group includes a positive single lens 201, and the third lens group includes a cemented lens composed of a biconvex lens 301 and a biconcave lens 302. It should be noted that the present application does not exclude that each lens group also includes other lenses.
The optical structure is described in detail below:
the first aspect is with respect to focal length: f (f) 1 F is the combined focal length of the first lens group 2 F is the combined focal length of the second lens group 3 F is the combined focal length of the microscope objective lens, and the focal length satisfies the following relation:
in a further possible embodiment of the present application,
the second aspect is with respect to refractive index: n (N) m1 Refractive index of the negative lens of the first lens group, N p1 The refractive index of the biconvex lens of the first lens group satisfies the relation: n (N) m1 -N p1 >0.12。
A third aspect is with respect to the abbe number and relative partial abbe number:
V dp1 for the Abbe's number, V, of the lenticular lenses of the first lens group dm1 An abbe number for a negative lens of the first lens group that satisfies the relationship: v (V) dp1 -V dm1 >12;
V dm2 For the dispersion coefficient, V, of the biconcave lens of the third lens group dp2 As the dispersion coefficient of the lenticular lens of the third lens group, it satisfies the relation: -10<V dm2 -V dp2 <10。
The partial dispersion coefficient, which is the ratio of the difference in refractive index of light at different wavelengths,the partial dispersion coefficient between g and f light. When the optical system is achromatic, the abbe number difference between the positive and negative lens glass materials is large, but after primary chromatic aberration is eliminated, high-order chromatic aberration, namely secondary spectrum, remains. Achromatic systems designed with ordinary optical glass typically have a certain secondary spectrum. In order to eliminate the secondary spectrum of the system, the complex optimization combination of glass materials with different partial dispersions is selected to obtain the effect of eliminating the secondary spectrum.
dP g,f >0.005, where dP g,f The relative partial dispersion coefficient of the positive single lens 201 of the second lens group is calculated as:
wherein n is g Refractive index of g line (436 nm), n f Refractive index of F line (486 nm), n d Refractive index of d-line (588 nm), n c The refractive index of the C line (656 nm).
In one embodiment, the relative partial dispersion coefficient of the lenticular lens 102 of the first lens group is also greater than 0.005.
A fourth aspect is related to the radius of curvature: the second lens can also comprise a single lens other than the positive single lens 201, the mirror surface of the second lens group closest to the object plane is a convex surface, R 1 The radius of curvature of the mirror surface closest to the object plane of the second lens group satisfies the relation:
the mirror surface closest to the image surface of the third mirror group is a concave surface R 2 The radius of curvature of the mirror surface closest to the image surface of the third lens group satisfies the relation:
in one possible embodiment, the radius of curvature satisfies
In yet another aspect, the object-side numerical aperture NA of the microscope objective is between 0.1 and 0.2.
In the embodiment of the application, the spatial distance relation between the microscope objective lens and the object plane and the mirror plane is as follows: d, d 0 The distance from the object plane to the object plane side mirror plane of the first mirror group satisfies the relation:
further optionally, a->
Examples:
the microscope objective lens is sequentially provided with a negative lens 101, a biconvex lens 102, a positive single lens 201, a biconvex lens 301 and a biconcave lens 302 from an object plane to a lens, and the optical axes of the lenses are coincident or almost coincident; microscope objective focal length f=40, object space numerical aperture na=0.15, working distance d 0 The maximum half image height hy=12.5 mm using a 200mm focal length tube lens=43.6 mm, and the specific optical parameters of the microscope objective lens are as follows:
wherein, surface 1 is the left side of negative lens 101 in fig. 1, surface 2 is the bonding surface between negative lens 101 and biconvex lens 102, surface 3 is the right side of biconvex lens 102, surface 4 is the left side of positive single lens 201, surface 5 is the right side of positive single lens 201, surface 6 is the left side of biconvex lens 301, surface 7 is the bonding surface between biconvex lens 301 and biconcave lens 302, and surface 8 is the right side of biconcave lens 302. The pitch in the above table indicates the distance from the current surface to the next adjacent surface, for example, the pitch of the surface 2 is 5mm, and indicates the pitch between the cemented surface between the negative lens 101 and the lenticular lens 102 and the right side surface of the lenticular lens 102 is 5mm, that is, the thickness of the lenticular lens 102 is 5mm; for another example, the pitch of the surface 3 is 0.249278mm, which means that the pitch between the right side surface of the lenticular lens 102 and the left side surface of the positive single lens 201 is 0.249278mm, that is, the air distance between the lenticular lens 102 and the positive single lens 201 is 0.249278mm.
The relative partial dispersion coefficient of the positive single lens 201 of the second lens group and the biconvex lens 102 of the first lens group is 0.0063.
The transfer function MTF diagram of the microscope objective is shown in fig. 2, the horizontal axis is spatial frequency, the unit is line pair/millimeter (lp/mm), two lines are calculated as a line pair, and the line pair which can be distinguished per millimeter is the numerical value of resolution; the vertical axis is modulation transmissionThe transfer function MTF (Modulation Transfer Function) is a quantitative description of the resolution of the lens. In the present embodiment, modulation (Modulation) is used to represent contrast, and the maximum brightness is defined as I max Minimum brightness is I min The modulation M is defined as: m= (I) max -I min )/(I max +I min ). The larger the modulation M is between 0 and 1, which means that the contrast is larger, and when the maximum luminance and the minimum luminance are completely equal, the contrast is completely lost, and the modulation M is equal to 0.
For a sine wave with an original modulation degree of M, if the modulation degree of an image reaching the image plane through the lens is M', the MTF function value is: MTF value = M' M. It can be seen that the MTF value must be between 0 and 1, and the closer to 1, the better the performance of the lens, if the MTF value of the lens is equal to 1, the modulation degree of the lens output completely reflects the contrast of the input sine wave; whereas if the modulation degree of the input sine wave is 1, the modulation degree of the output image is exactly equal to the MTF value, so the MTF function represents the contrast of the lens at a certain spatial frequency.
As can be seen from the MTF curves of fig. 2, the MTF values of the microscope objective in this example at a representative 0 field of view, 0.5 field of view and maximum field of view are already very close to the diffraction limit. The diffraction limit means that when an ideal object point is imaged by an optical system, it is impossible to obtain an ideal image point due to the limitation of diffraction of light of physical optics, but a diffraction image of the diffraction image of the diffraction system is obtained, and the diffraction image is the diffraction limit of the physical optics, that is, the maximum value.
In fig. 2, the diffraction limit is shown as TS DIFF LIMIT, and it can be seen that the microscope objective lens in this embodiment can be used to make the MTF value near the diffraction limit of the physical optics over a wide visible spectrum, i.e. the imaging quality index near the limit under ideal conditions, which indicates that the imaging quality is very good.
The microscope objective lens of the embodiment has a small number of lenses, good processing performance and excellent imaging performance, and has a wide application prospect.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The foregoing is merely illustrative of the embodiments of this application and it will be appreciated by those skilled in the art that variations and modifications may be made without departing from the principles of the application, and it is intended to cover all modifications and variations as fall within the scope of the application.
Claims (9)
1. A microscope objective configured as an infinity objective of imaging magnification and long working distance of 4 to 6 times, characterized by consisting of a first lens group, a second lens group and a third lens group arranged in order along an optical axis in an object-plane-to-image-plane direction, wherein the first lens group consists of a negative lens (101) and a biconvex lens (102) arranged in order along the optical axis in the object-plane-to-image-plane direction, the second lens group is a positive single lens (201), and the third lens group consists of a biconvex lens (301) and a biconcave lens (302) arranged in order along the optical axis in the object-plane-to-image-plane direction;
wherein the negative lens (101) and the biconvex lens (102) of the first lens group form a cemented lens, and the biconvex lens (301) and the biconcave lens (302) of the third lens group form a cemented lens;
the microscope objective satisfies the relationship:
5.2<f 1 /f<7.9;
0.5< f 2 /f <2;
1.6<-f 3 /f<2.4;
wherein,f 1 for the combined focal length of the first lens group,f 2 for the combined focal length of the second lens group,f 3 for the combined focal length of the third lens group,ffor a combined focal length of the microscope objective.
2. The microscope objective of claim 1, wherein an optical axis spacing between the first and second lens groups is less than or equal to 3mm and an optical axis spacing between the second and third lens groups is less than or equal to 3mm.
3. Microscope objective according to claim 1, characterized in that it satisfies the relation:
N m 1 -N p1 >0.12, wherein,N m1 is the refractive index of the negative lens (101) of the first lens group,N p1 a refractive index of a lenticular lens (102) of the first lens group; and/or the number of the groups of groups,
V dp 1 -V dm1 >12, wherein,V dp1 for the abbe number of the lenticular lenses (102) of the first lens group,V dm1 is the dispersion coefficient of the negative lens (101) of the first lens group.
4. Microscope objective according to claim 1, characterized in that it satisfies the relation:
-10< V dm2 - V dp2 <10, wherein,V dm2 is the dispersion coefficient of the biconcave lens (302) of the third lens group,V dp2 is the dispersion coefficient of the lenticular lens (301) of the third lens group.
5. Microscope objective according to claim 1, characterized in that it satisfies the relation: 0.25<R 1 /f<1.1, wherein,R 1 a radius of curvature of an object side surface of the positive single lens (201); and/or the number of the groups of groups,
it satisfies the relation: 0.15<R 2 /f<0.6, wherein,R 2 is the radius of curvature of the image side of the biconcave lens (302) of the third lens group.
6. Microscope objective according to claim 1, characterized in that it satisfies the relation:
dP g,f >0.005 of the total number of the components, wherein,dP g,f the relative partial dispersion coefficient of the positive single lens (201) of the second lens group is calculated as follows:
dP g,f =(n g -n f )/(n f -n c )-0.6457+0.001703*(n d -1)/(n f -n c ) Wherein, the method comprises the steps of, wherein,n g the refractive index of the positive single lens (201) at g line,n f is at the positive single lens (201)FThe refractive index of the line is such that,n d is at the positive single lens (201)dThe refractive index of the line is such that,n c is at the positive single lens (201)CRefractive index of the line.
7. Microscope objective according to one of claims 1 to 6, characterized in that it satisfies the relation:
0.55<d 0 /f<2.2, wherein,d 0 is the distance along the optical axis from the object plane to the object plane of the negative lens (101) of the first lens group.
8. Microscope objective according to one of claims 1 to 6, characterized in that it satisfies the relation:
0.1<NA<0.2, wherein,NAan object numerical aperture for the microscope objective.
9. The microscope objective according to any one of claims 1 to 7, characterized in that the ratio of the distance of the object plane to the object side of the negative lens (101) of the first lens group along the optical axis to the combined focal length of the microscope objective is between 0.85 and 1.25;
the microscope objective satisfies the relationship:
0.42<R 1 /f<0.66,0.24<R 2 /f<0.45, wherein,R 1 is the radius of curvature of the object side surface of the positive single lens (201) of the second lens group,R 2 a radius of curvature of an image side surface of the biconcave lens (302) that is the third lens group;
0.75<f 2 /f<1.2; wherein,f 2 for the combined focal length of the second lens group,ffor a combined focal length of the microscope objective.
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CN111381354A (en) * | 2020-03-04 | 2020-07-07 | 宁波舜宇仪器有限公司 | Microscope objective |
CN112578545A (en) * | 2020-11-11 | 2021-03-30 | 南京星烁光学仪器有限公司 | 40-time long working distance optical microscope objective |
CN114002817A (en) * | 2021-11-09 | 2022-02-01 | 张家港中贺自动化科技有限公司 | Microscope objective |
CN114019665A (en) * | 2021-12-14 | 2022-02-08 | 宁波舜宇仪器有限公司 | Microscope objective |
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CN111381354A (en) * | 2020-03-04 | 2020-07-07 | 宁波舜宇仪器有限公司 | Microscope objective |
CN112578545A (en) * | 2020-11-11 | 2021-03-30 | 南京星烁光学仪器有限公司 | 40-time long working distance optical microscope objective |
CN114002817A (en) * | 2021-11-09 | 2022-02-01 | 张家港中贺自动化科技有限公司 | Microscope objective |
CN114019665A (en) * | 2021-12-14 | 2022-02-08 | 宁波舜宇仪器有限公司 | Microscope objective |
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