CN115198029A - Small spinach fingerprint spectrum, construction method and application thereof - Google Patents
Small spinach fingerprint spectrum, construction method and application thereof Download PDFInfo
- Publication number
- CN115198029A CN115198029A CN202210641480.4A CN202210641480A CN115198029A CN 115198029 A CN115198029 A CN 115198029A CN 202210641480 A CN202210641480 A CN 202210641480A CN 115198029 A CN115198029 A CN 115198029A
- Authority
- CN
- China
- Prior art keywords
- spinach
- fingerprint
- bands
- ssr
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Botany (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Theoretical Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a small spinach fingerprint, a construction method and application thereof, wherein the small spinach fingerprint is obtained by performing PCR amplification on DNA of a small spinach sample by using the following primers, performing electrophoresis detection on a PCR product, and finally performing data analysis. The fingerprint of the spinach disclosed by the invention provides a scientific basis for screening valuable breeding materials from local variety germplasm resources, reduces the breeding blindness, improves the breeding efficiency of the spinach, provides a basis for the protection of new variety intellectual property and variety identification and innovative breeding of the spinach, and also provides a theoretical support for increasing the number of spinach fingerprints, enriching the number of SSR markers and perfecting a spinach DNA fingerprint spectrum database.
Description
Technical Field
The invention belongs to the field of biotechnology and botany, and particularly relates to a small spinach fingerprint spectrum, a construction method and application thereof.
Background
Simple Sequence Repeats (SSRs), also known as microsatellites, are DNA fragments with tandem repeat motifs of 1-6 nucleotides. Wide genome coverage, robustness and high reproducibility, CO dominant inheritance, high polymorphism of multiple alleles per site, transferability between species, and low requirements for expertise and instrumentation are some of the attractive features of SSR markers. Therefore, genotyping plants using SSR markers is relatively inexpensive and can be used in small laboratories. SSR markers have been applied to fingerprinting, genetic diversity, population structure analysis, association mapping, and linkage mapping.
Spinach is native to ancient Persia and has spread around the world since the 11 th century. Through the active or passive selection of isolation of different regions and climates for a long time, the genetic diversity of spinach is quite abundant. The method comprises the following steps that Li (2018) excavates 85 polymorphic SSR markers from a genome sequence of one spinach variety, carries out genotyping on 48 spinach varieties in the world, and discovers spinach variety clustering based on geographical sources; xu (2017) and the like develop 34 new polymorphic SSR markers from spinach reference genomes and are used for evaluating genetic diversity of Chinese spinach germplasm resources, and the experimental research result shows that: spinach has a genetic similarity coefficient of 0.30-0.75 and a percent of 89.90% polymorphic sites, which may be attributed to: the information quantity of the RAPD and AFLP molecular markers with extremely high polymorphism obtained under the condition of the same number of molecular markers is more than 8-10 times that of the SSR markers, so that the obtained information quantity can not explain the genetic diversity displayed on the whole genome under the condition of low SSR abundance, and the similarity coefficient result obtained by the RAPD and the AFLP is higher than that of the SSR molecular markers. Khattak (2007) uses 13 SSR markers to research the genetic diversity of 33 hybrid spinach varieties, and the research finds that the varieties can be divided into three genetic groups according to different producing areas. In another study, kuwahara (2014) investigated genetic diversity of 50 spinach germplasm using a set of six SSR markers, and the results showed that spinach germplasm has higher genetic diversity, with the highest genetic diversity of material from west asia (afghanistan, iran, irak and syria).
The DNA fingerprinting technology is a DNA electrophoresis map which is based on DNA molecular markers and can identify the differences of biological individuals, and mainly comprises the fingerprinting technology based on AFLPRFLP, RAPD, SSR, SNP and other markers, wherein SSR and SNP markers are most commonly used. The fingerprint constructed by using SSR molecular markers contains a large amount of related information, and the technical variety identification field is widely applied due to the characteristics of convenience in modification, simplicity, high efficiency and the like. Cantini et al (2001) established a fingerprint for 59 cherry varieties by using a high polymorphism SSR molecular marker technology; 8 pairs of SSR primers with high polymorphism and stable amplification are combined by the Wangxing star and the like (2017) to successfully construct the fingerprint of 48 fruit ginkgo varieties; the method comprises the following steps of conducting amplification on a stable polymorphic band obtained by 18 pairs of SSR core primers with obvious polymorphic bands, and successfully constructing a fingerprint spectrum library for 105 parts of melon materials by utilizing the method (2012). The research of Zhao Rui Li utilizes 7 pairs of SSR primers screened out, and a fingerprint is constructed for 33 spinach germplasms according to 67 polymorphic band types obtained by the SSR primers. In another study, kuwahara (2014) used a set of six SSR markers to investigate the genetic diversity of 50 spinach germplasm. The results indicate that spinach germplasm has a high genetic diversity, with the highest genetic diversity of material from west asia (afghanistan, iran, iraq and syria). At present, systematic research on genetic diversity of Chongming spinach populations has not been found.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to provide a small spinach fingerprint spectrum, a construction method and application thereof.
The technical scheme is as follows: in order to solve the technical problems, the invention adopts the following technical scheme:
the fingerprint of the little spinach is obtained by adopting the following primers to perform PCR amplification on DNA of a little spinach sample, performing electrophoresis detection on a PCR product and finally performing data analysis;
the names and sources of the spinach samples are shown in the following table:
the spinach fingerprint is shown in the following table:
wherein, according to the electrophoresis result, the SSR amplification bands are counted, and at the same migration position, the band with the SSR amplification band is marked as '1', and the band without the SSR amplification band is marked as '0'.
The construction method of the fingerprint of the spinach comprises the following steps:
(1) Amplifying 42 parts of spinach DNA by using SSR primers, and selecting SSR primers with clear bands and high polymorphism from the spinach DNA;
(2) Analyzing the obtained polymorphic loci and finding out primers capable of distinguishing 42 spinach samples;
(3) And (3) using the primer selected in the step (2) as a core primer for constructing fingerprints, and respectively marking the amplified fragments with '1' and '0' at the same migration position to construct the fingerprint maps of 42 spinach samples.
Preferably, in step (1), the amplification system is 20 μ L, and comprises: 1 mu L of DNA template; 1. Mu.L of each of the upstream and downstream primers (10 ng. Mu.L); PCR Master Mix 15. Mu.L; add ddH2O to 20. Mu.L.
Preferably, in step (3), the SSR amplification bands are counted based on the electrophoresis results, and the bands are represented as "1" for the bands and "0" for the bands at the same migration positions, thereby forming respective data matrices. And calculating the total bands amplified according to each pair of primers and the number of polymorphic bands by utilizing POPGENE32 software according to the statistical result, and calculating the polymorphic percentage. The data were statistically analyzed for the index of genetic diversity (He) hannon information (I) and the coefficient of genetic similarity of 42 spinach test seed materials (Nei, 1973) using the POPGENE2.10 software.
The invention also provides application of the small spinach fingerprint in spinach variety identification.
The invention finally provides an identification method of spinach varieties, which comprises the following steps:
(1) Extracting the genomic DNA of the purple spinach to be detected, and performing PCR amplification on the genomic DNA of the sample to be detected by using the primers;
(2) Carrying out electrophoresis detection on the PCR amplification product;
(3) Counting SSR amplification bands according to the electrophoresis result, recording the bands as '1' and the bands as '0' at the same migration position, and forming respective data matrixes;
(4) And (4) comparing the result of the step (3) with the fingerprint of the little spinach to identify the variety.
Has the advantages that: the fingerprint of the spinach disclosed by the invention provides a scientific basis for screening valuable breeding materials from local variety germplasm resources, reduces breeding blindness, improves spinach breeding efficiency, provides a basis for protection of new variety intellectual property and variety identification and innovative breeding of the spinach, and also provides a theoretical support for increasing the number of spinach fingerprints, enriching the number of SSR markers and perfecting a spinach DNA fingerprint database.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to the following examples. The experimental methods used in the examples are conventional methods unless otherwise specified, and the materials, reagents and the like used therein are commercially available.
Examples
1. SSR primer screening and PAGE electrophoresis
The SSR primers are respectively from published sequences and synthesized by Shanghai Biotechnology Limited. The DNA of 42 parts of spinach (table 1) is amplified by 300 parts of SSR primers, and 57 pairs of SSR primers with clear bands and high polymorphism are selected from the DNA (table 2). The amplification system is 20 mu L, namely 1 mu L of DNA template; 1. Mu.L of each of the upstream and downstream primers (10 ng. Mu.L); PCR Master Mix 15. Mu.L; add ddH 2 O to 20. Mu.L. The electrophoresis of the PCR product PAGE gel refers to the method of Togaele et al (2014). SSR primers, agarose and a PCR Master Mix kit are purchased from Shanghai Biotechnology engineering Co., ltd. The main equipment comprises: PCR amplification (PCR apparatus model: mycycler PCR apparatus, bio-Rad, USA).
TABLE 1 spinach Material names and sources
Table 2: primer sequence information
2. SSR fingerprint construction
Analyzing the obtained polymorphic loci. The results showed that the 9 pairs of primers (9, 15, 34, 40, 44, 46, 47, 52 and 56, table 3) could distinguish between 42 materials. Nine SSR-labeled primers were used as core primers for constructing fingerprints. At the same migration position, the amplified fragments are marked by '1' and '0' respectively, and the fingerprint maps of 42 spinach resources are constructed. The specific strip combination of 9 pairs of primers is used as the DNA fingerprint of one variety and can be used for identifying different varieties.
Table 3: basic information of 9 pairs of core primers
Molecular marking: counting SSR amplification bands according to electrophoresis results, recording the bands as '1' and the bands as '0' at the same migration positions, and forming respective data matrixes. And calculating the total bands amplified according to each pair of primers and the number of the polymorphic bands by utilizing POPGENE32 software according to the statistical result, and calculating the polymorphism percentage. The data were statistically analyzed for the index of genetic diversity (He) hannon information (I) and the coefficient of genetic similarity of 42 spinach test seed materials (Nei, 1973) using the POPGENE2.10 software.
3. Fingerprint spectrum
With the diversification of market demands, the types of spinach varieties are gradually increased, and the difference between the varieties is gradually reduced. Are difficult to identify by means of phenotypic features alone. The fingerprint constructed by the molecular marker contains more related information and can be modified at any time. Has the characteristics of high efficiency and convenience, and is favorable for variety identification. This technique has been widely used. In the research, 9 pairs of SSR primers (No. 9, 15, 34, 40, 44, 46, 47, 52 and 56) which are stable in amplification and obvious in polymorphic bands are screened out. Based on the 63 polymorphic bands obtained, a fingerprint of 42 spinach germplasm was constructed (Table 4). Primers 9 and 46 can distinguish Chongming spinach SP35 (Chongming spinach) and SP26 from other varieties. The primer 40 can distinguish SP35 (Chongming spinach) and SP40 from other varieties. The pic values of primers satt083 and satt534 are both more than 0.65, and the Shannon Weaver diversity index is more than 1.78, which shows that the two pairs of primers have stronger variety identification capability and can be preferentially used for spinach variety identification. Because of the limited choice of primers and materials in this study, fingerprints were constructed that were limited to distinguishing the selected materials. If we want to increase the population we need to reselect primers and go on further study.
Table 4 shows that the fingerprint of 42 spinach resources constructed by 9 pairs of SSR primers is shown in the specification
The above embodiments are the best mode for carrying out the invention, but the embodiments of the invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the invention should be construed as equivalents thereof, and they are included in the scope of the invention.
Sequence listing
<110> Shanghai city academy of agricultural sciences
Vegetable science and technology promotion station in Shanghai city Chongming area
<120> small spinach fingerprint spectrum, construction method and application thereof
<160> 18
<170> SIPOSequenceListing 1.0
<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
actagtgagg gggccagttt aca 23
<210> 2
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
cagctgaggc tcttcttctt cttc 24
<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
aggtagaggc aaaggaagag gca 23
<210> 4
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
acagaaccgg aaaaaaaaaa aggg 24
<210> 5
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 5
ttagcaaaca ccaaaaattg ga 22
<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 6
gaacaccaaa tgttgcatgg 20
<210> 7
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 7
ccatgaggaa caacactagc aa 22
<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 8
ccatctcaca gacaatctca cag 23
<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 9
cttgcctcca atttgcattt 20
<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 10
tgtgagagtc ggagaatcct t 21
<210> 11
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 11
atgaaggcaa gatttcggta ct 22
<210> 12
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 12
ctcaaccacg acatgaacct ta 22
<210> 13
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 13
cctcatcatt gtcctcatca ag 22
<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 14
actgccgaga actgcataaa ct 22
<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 15
aaccaaaagg caacaaaaat g 21
<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 16
ttttgaacaa ccccgatagc 20
<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 17
atctcgtgcc aaccataacc 20
<210> 18
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 18
ttgatcagag gacaaagaaa gc 22
Claims (8)
1. The fingerprint of the little spinach is characterized in that the primers are adopted to carry out PCR amplification on DNA of a little spinach sample, the PCR product is subjected to electrophoresis detection, and finally, data analysis is carried out to obtain the little spinach fingerprint;
2. the spinach fingerprint as claimed in claim 1, wherein the spinach samples have the names and sources as shown in the following table:
3. spinach fingerprint as claimed in claim 1, characterized in that the spinach fingerprint is as shown in the following table:
wherein, according to the electrophoresis result, the SSR amplification bands are counted, and at the same migration position, the band with the SSR amplification band is marked as '1', and the band without the SSR amplification band is marked as '0'.
4. The method for constructing the fingerprint of spinach as claimed in claim 1, which comprises the following steps:
(1) Amplifying 42 parts of spinach DNA by using SSR primers, and selecting SSR primers with clear bands and high polymorphism from the spinach DNA;
(2) Analyzing the obtained polymorphic loci and finding out primers capable of distinguishing 42 spinach samples;
(3) And (3) using the primer selected in the step (2) as a core primer for constructing fingerprints, and respectively marking the amplified fragments with '1' and '0' at the same migration position to construct the fingerprint maps of 42 spinach samples.
5. The method for constructing the fingerprint of spinach as claimed in claim 4, wherein in the step (1), the amplification system is 20 μ L and comprises the following steps: 1 mu L of DNA template; 1. Mu.L of each of the upstream and downstream primers (10 ng. Mu.L); PCRmaster Mix15 μ L; add ddH2O to 20. Mu.L.
6. The method for constructing a fingerprint of spinach as claimed in claim 4, wherein in the step (3), the SSR amplified bands are counted according to the electrophoresis result, and the bands are marked as "1" and the bands are not marked as "0" at the same migration positions, so as to form respective data matrixes. And calculating the total bands amplified according to each pair of primers and the number of the polymorphic bands by utilizing POPGENE32 software according to the statistical result, and calculating the polymorphism percentage. The data were statistically analyzed for the index of genetic diversity (He) hannon information (I) and the coefficient of genetic similarity of 42 spinach test seed materials (Nei, 1973) using the POPGENE2.10 software.
7. Use of a fingerprint of a spinach as claimed in claim 1 for the identification of a spinach variety.
8. The method for identifying the spinach variety is characterized by comprising the following steps of:
(1) Extracting the genomic DNA of purple spinach to be detected, and respectively carrying out PCR amplification on the genomic DNA of a sample to be detected by using the primers as claimed in claim 1;
(2) Carrying out electrophoresis detection on the PCR amplification product;
(3) Counting SSR amplification bands according to the electrophoresis result, recording the bands as '1' and the bands as '0' at the same migration position, and forming respective data matrixes;
(4) And (3) comparing the result of the step (3) with the fingerprint of the spinach as claimed in any one of claims 1 to 3 to carry out variety identification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210641480.4A CN115198029B (en) | 2022-06-07 | 2022-06-07 | Small spinach fingerprint, construction method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210641480.4A CN115198029B (en) | 2022-06-07 | 2022-06-07 | Small spinach fingerprint, construction method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115198029A true CN115198029A (en) | 2022-10-18 |
| CN115198029B CN115198029B (en) | 2023-08-08 |
Family
ID=83576235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210641480.4A Active CN115198029B (en) | 2022-06-07 | 2022-06-07 | Small spinach fingerprint, construction method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115198029B (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003085133A2 (en) * | 2002-04-08 | 2003-10-16 | Centre For Dna Fingerprinting And Diagnostics | Novel fissr-pcr primers and method of genotyping diverse genomes of plant and animal systems including rice varieties |
| CN1944649A (en) * | 2006-10-27 | 2007-04-11 | 上海市农业科学院 | Vegetable soy bean DNA finger print map and its construction method and use |
| CN102719554A (en) * | 2012-07-17 | 2012-10-10 | 天津市农业质量标准与检测技术研究所 | Method for building celery EST-SSR (expressed sequence tags-simple sequence repeat) fingerprint and application thereof |
| CN105420368A (en) * | 2015-12-17 | 2016-03-23 | 黑龙江八一农垦大学 | Method for constructing fingerprint of phaseolus vulgaris according to SSR (simple sequence repeat) molecular marker and application |
| CN106978504A (en) * | 2017-05-03 | 2017-07-25 | 上海师范大学 | A kind of preparation method and application of spinach SSR marker |
| CN108165655A (en) * | 2018-02-27 | 2018-06-15 | 河南师范大学 | A kind of SSR marker SP_SSR19 with spinach male close linkage and its application in spinach sex identification |
| CN110438252A (en) * | 2019-08-02 | 2019-11-12 | 中国农业科学院蔬菜花卉研究所 | Molecular labeling and its application with spinach male and female gender close linkage |
| CN110453003A (en) * | 2019-08-02 | 2019-11-15 | 中国农业科学院蔬菜花卉研究所 | Molecular labeling and its application with spinach leaf character main effect QTL compact linkage |
| CN112575108A (en) * | 2020-12-21 | 2021-03-30 | 上海市农业科学院 | SSR molecular marker combination, SSR primer combination, SSR fingerprint and application of SSR molecular marker combination and SSR primer combination |
| CN113373259A (en) * | 2021-08-02 | 2021-09-10 | 黑龙江八一农垦大学 | Common kidney bean fingerprint constructed based on phenotype and SSR molecular marker combination |
-
2022
- 2022-06-07 CN CN202210641480.4A patent/CN115198029B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003085133A2 (en) * | 2002-04-08 | 2003-10-16 | Centre For Dna Fingerprinting And Diagnostics | Novel fissr-pcr primers and method of genotyping diverse genomes of plant and animal systems including rice varieties |
| CN1944649A (en) * | 2006-10-27 | 2007-04-11 | 上海市农业科学院 | Vegetable soy bean DNA finger print map and its construction method and use |
| CN102719554A (en) * | 2012-07-17 | 2012-10-10 | 天津市农业质量标准与检测技术研究所 | Method for building celery EST-SSR (expressed sequence tags-simple sequence repeat) fingerprint and application thereof |
| CN105420368A (en) * | 2015-12-17 | 2016-03-23 | 黑龙江八一农垦大学 | Method for constructing fingerprint of phaseolus vulgaris according to SSR (simple sequence repeat) molecular marker and application |
| CN106978504A (en) * | 2017-05-03 | 2017-07-25 | 上海师范大学 | A kind of preparation method and application of spinach SSR marker |
| CN108165655A (en) * | 2018-02-27 | 2018-06-15 | 河南师范大学 | A kind of SSR marker SP_SSR19 with spinach male close linkage and its application in spinach sex identification |
| CN110438252A (en) * | 2019-08-02 | 2019-11-12 | 中国农业科学院蔬菜花卉研究所 | Molecular labeling and its application with spinach male and female gender close linkage |
| CN110453003A (en) * | 2019-08-02 | 2019-11-15 | 中国农业科学院蔬菜花卉研究所 | Molecular labeling and its application with spinach leaf character main effect QTL compact linkage |
| CN112575108A (en) * | 2020-12-21 | 2021-03-30 | 上海市农业科学院 | SSR molecular marker combination, SSR primer combination, SSR fingerprint and application of SSR molecular marker combination and SSR primer combination |
| CN113373259A (en) * | 2021-08-02 | 2021-09-10 | 黑龙江八一农垦大学 | Common kidney bean fingerprint constructed based on phenotype and SSR molecular marker combination |
Non-Patent Citations (3)
| Title |
|---|
| 张婉;崔继哲;于拴仓;李丽;: "白菜品种的SSR指纹图谱数据库的构建", 分子植物育种 * |
| 潜宗伟;陈海丽;崔彦玲;: "菠菜转录组SSR位点分析及其分子标记的开发", 农业生物技术学报 * |
| 阎君，等: "基于转录组测序的菠菜SSR标记开发", 园艺学报 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115198029B (en) | 2023-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Montemurro et al. | Genetic relationships and cultivar identification among 112 olive accessions using AFLP and SSR markers. | |
| CN110241252B (en) | SNP molecular marker combination for constructing peach DNA fingerprint spectrum, application and method | |
| Minaeifar et al. | Biosystematic study in the genus Cousinia Cass.(Asteraceae), section Cousinia | |
| Nimmakayala et al. | Use of VeraCode 384-plex assays for watermelon diversity analysis and integrated genetic map of watermelon with single nucleotide polymorphisms and simple sequence repeats | |
| CN108220402B (en) | Method for identifying pedigree relationship between Chinese cabbage germplasm and variety | |
| CN108588255B (en) | Indel marker development for distinguishing five pepper cultivars and application thereof | |
| Pratap et al. | Cross‐genera amplification of informative microsatellite markers from common bean and scarlet runner bean for assessment of genetic diversity in mungbean (Vigna radiata) | |
| CN104598773A (en) | Method for developing endangered rhododendron molle SSR primer based on RAD-seq | |
| Liu et al. | Characterization of new microsatellite markers based on the transcriptome sequencing of Clematis finetiana | |
| CN110551844B (en) | Sugarcane cultivar genome SSR molecular marker development method and application | |
| CN110106279B (en) | Single-site SSR primer group developed based on Miscanthus formosanus genome sequence and application thereof | |
| CN115852022B (en) | Tobacco core SNP marker developed based on whole genome resequencing and KASP technology and application thereof | |
| CN111471791A (en) | Soybean DNA fingerprint construction method and application thereof | |
| WO2017088144A1 (en) | Snp combination for analyzing diversity of chinese cabbage germplasm resource and for molecular breeding, and use thereof | |
| CN115198029A (en) | Small spinach fingerprint spectrum, construction method and application thereof | |
| Xiaoying et al. | A novel strategy employed in identification of 25 important loquat cultivars using RAPD marker | |
| CN114317800B (en) | EST-SSR marker primer developed based on biota orientalis transcriptome sequence and application thereof | |
| CN113897457A (en) | KASP molecular marker linked with wheat stripe rust resistant QTL and application thereof | |
| CN114807413A (en) | Olive ISSR-PCR molecular marker combination and application thereof | |
| CN109280722B (en) | Chinese pumpkin transcriptome SSR molecular marker primer and application thereof | |
| Hosang et al. | Genetic variability in West Timor landrace maize populations | |
| CN113215297A (en) | Molecular marker ID0159 closely linked with major QTL site of sesame oil content and application thereof | |
| CN115976251B (en) | InDel marker for cassava genetic diversity analysis and application thereof | |
| CN115873986B (en) | EST-SSR molecular marker primer group and application thereof | |
| CN116716423B (en) | EST-SSR marker primer pair developed based on phalaris arundinacea transcriptome sequence, and phalaris arundinacea variety identification method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20230712 Address after: 201106 No. 2901 Zhai Road, Minhang District, Shanghai Applicant after: Shanghai Academy of Agricultural Sciences Applicant after: Shanghai Chongming vegetable science and Technology Extension Station Applicant after: SHANGHAI SUNQIAO OVERFLOW BETTER AGRICULTURAL TECHNOLOGY Co.,Ltd. Address before: 201106 No. 2901 Zhai Road, Minhang District, Shanghai Applicant before: Shanghai Academy of Agricultural Sciences Applicant before: Shanghai Chongming vegetable science and Technology Extension Station |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |