CN115181732A - Preparation method of phage lyase source feed additive capable of effectively removing impurities - Google Patents
Preparation method of phage lyase source feed additive capable of effectively removing impurities Download PDFInfo
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- CN115181732A CN115181732A CN202210863436.8A CN202210863436A CN115181732A CN 115181732 A CN115181732 A CN 115181732A CN 202210863436 A CN202210863436 A CN 202210863436A CN 115181732 A CN115181732 A CN 115181732A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/00031—Uses of virus other than therapeutic or vaccine, e.g. disinfectant
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/00051—Methods of production or purification of viral material
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to the technical field of feed additives, and discloses a preparation method of a phage lyase source feed additive for effectively removing impurities, which comprises the following steps: s1: preparing a culture medium, putting MgSO47H2O, KH2PO4, naCl, beer yeast, yeast extract powder, glucose, thiamine hydrochloride, lignin powder and bran into a mixing tank, and stirring and mixing to obtain the culture medium; s2: preparing a phage stock solution, namely adding calcium chloride, magnesium chloride, banana puree and active carbon into the prepared culture medium serving as a raw material to mix, and preparing the phage stock solution; s3: preparing the auxiliary materials. According to the invention, calcium chloride, magnesium chloride, banana puree and active carbon are added to react with each other, so that the raw materials are more nutritious while removing peculiar smell and impurities, the culture efficiency is improved, impurities added into the raw materials can be controlled through impurity removal treatment, the impurities are prevented from influencing culture and fermentation of a culture medium, and the impurity removal effect is improved.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a preparation method of a phage lyase source feed additive for effectively removing impurities.
Background
The bacteriophage is a specific virus infecting bacteria, and is also a most abundant and most various biological entity on the earth, and the number of the bacteriophage in a biosphere is estimated to be up to 1031, and the bacterial death rate caused by bacteriophage infection and lysis can account for 20-40% of the total death rate. Thus, bacteriophages have a considerable influence on the biogeochemical cycle. The life history of the phage includes the lysis cycle and the lysogenic cycle. After the phage entering the lysis cycle infects the host, a large amount of host substances are utilized to synthesize and assemble progeny phage, and the host cells are lysed to release progeny viruses.
The bacteriophage grows and breeds in host cells, can cause the lysis of pathogenic bacteria, and reduce the density of the pathogenic bacteria, thereby reducing or avoiding the infection or morbidity of the pathogenic bacteria and achieving the purpose of treating and preventing diseases, namely bacteriophage therapy. The therapy is widely applied to the fields of veterinarians, agriculture, food microbiology and the like, and the domestic breeding industry, especially the chicken industry, is often troubled by the livestock and poultry intestinal diarrhea, which is mainly caused by pathogenic microorganisms such as escherichia coli, salmonella and the like. With the emergence of drug-resistant bacteria, the treatment of bacterial diseases by using bacteriophage with strong specificity and resistance is important. Smith, barrow and the like can reduce the probability of suffering from escherichia coli intestinal diseases of lambs, piglets and chicks by utilizing phage therapy, and the conventional phage lyase feed additive has a lot of impurities in the preparation process, influences the whole using effect, is high in cost and cannot meet the requirements of people, so that the preparation method of the phage lyase feed additive for effectively removing the impurities is provided.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a preparation method of a phage lyase-derived feed additive for effectively removing impurities, and solves the problems that the existing phage lyase-derived feed additive has a lot of impurities in the preparation process, influences the overall use effect, is high in cost and cannot meet the requirements of people.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of a phage lyase source feed additive capable of effectively removing impurities comprises the following steps:
s1: preparing culture medium, putting MgSO47H2O, KH2PO4, naCl, beer yeast, yeast extract powder, glucose, thiamine hydrochloride, lignin powder and bran into a mixing tank, stirring and mixing to obtain culture medium;
s2: preparing a bacteriophage stock solution, taking the prepared culture medium as a raw material, adding calcium chloride, magnesium chloride, banana mud and active carbon for mixing, and fishing out the floating substances floating on the upper layer to prepare the bacteriophage stock solution;
s3: preparing auxiliary materials, namely culturing bacteriophage, preparing a production strain from the bacteriophage, performing lysis treatment on the strain, collecting lysate for later use, performing sterilization treatment on bacteriophage stock solution after induction, treating the lysate for later use, adding an induction buffer solution during induction for induction, wherein the addition amount of the induction buffer solution is 110-180mol/L of Na2HPO4, 120-200mmol/L of CaCl2 and 66-150mmol/L of MgCl2;
s4: performing amplification culture fermentation, namely taking a bacteriophage lysate and a bacteriophage stock solution after induced sterilization as raw materials, performing inclined plane amplification culture treatment on strains, adding the bacteriophage stock solution during amplification culture, then putting the strains into eggplant bottle seeds for amplification culture treatment, putting the strains into a seed fermentation tank for fermentation treatment after treatment, and putting the strains into the fermentation tank for secondary fermentation after fermentation;
s5: removing impurities, namely filtering and removing impurities when phage lysate and phage stock solution after induced sterilization are added each time during propagation fermentation, and controlling the addition amount;
s6: centrifuging and recovering, centrifuging after secondary fermentation, filtering with a purification and concentration membrane of microfiltration and ultrafiltration during centrifugation, collecting thallus, recovering the residual centrifugate of the thallus, and using the recovered liquid as bacteriophage stock solution for later use;
s7: purifying and concentrating, namely purifying and concentrating the thalli collected after centrifugation, and concentrating the live bacterial sludge;
s8: mixing and adsorbing, then performing mixing and adsorbing, and performing matching and adsorbing by adopting a special carrier gel during adsorbing;
s9: drying and crushing, namely putting the adsorbed material into a drying chamber for drying, crushing after drying, and filtering, removing impurities and crushing for multiple times while crushing to obtain a feed additive finished product.
As a further scheme of the invention, the S1 is added with glucose and thiamine hydrochloride slowly when preparing the culture medium, and is added with stirring when adding.
Further, BSA, lentinan, ganoderan and tea polyphenol are added and mixed in the S2 during the preparation of the phage stock solution.
On the basis of the scheme, the expanding culture is carried out in S4, the expanding culture is carried out at the heating temperature of 30-40 ℃, and then intermittent stirring treatment is carried out.
And further, filtering the impurities in the S5 through a common filtering gauze, and collecting the filtered impurities for later use.
On the basis of the scheme, the centrifugate in the S6 is filtered again when being recovered, so that large-particle impurities are removed.
(III) advantageous effects
Compared with the prior art, the invention provides the preparation method of the phage lyase feed additive for effectively removing impurities, and the preparation method has the following beneficial effects:
1. according to the invention, calcium chloride, magnesium chloride, banana puree and active carbon are added to enable the calcium chloride, the magnesium chloride, the banana puree and the active carbon to react with each other, so that the banana puree is more nutritious while removing peculiar smell and impurities, and the culture efficiency is improved.
2. According to the invention, the yield can be greatly improved through secondary expanding culture treatment, and the secondary fermentation treatment and the multi-strain fermentation process in the same fermentation tank solve the problems of large investment and low production efficiency of single-strain fermentation.
3. According to the invention, the bacteriophage lysate and the bacteriophage stock solution after induced sterilization are added each time during propagation fermentation, and filtration and impurity removal treatment are carried out, so that impurities added in raw materials can be controlled through impurity removal treatment, the impurities are prevented from influencing culture and fermentation of a culture medium, and the impurity removal effect is improved.
4. According to the invention, the purification and concentration membrane with microfiltration and ultrafiltration is adopted for filtration, so that impurities can be removed more effectively, the complete structure of the phage can be preserved, small molecular proteins and other various nutrients in the solution can be separated, the concentration of a target substance can be effectively improved, the shelf life can be prolonged, harmful impurities can be effectively removed through the processes of fermentation, impurity removal, purification and concentration, the content of the target substance can be improved, the biological activity can be maintained for a long time, the production cost is effectively reduced while the production efficiency is improved, the problem that the phage is easy to inactivate is solved by adopting the gel adsorption of a special carrier, and the storage life of the phage is prolonged.
Drawings
FIG. 1 is a schematic flow structure diagram of a method for preparing a phage lyase-derived feed additive for effectively removing impurities according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
Referring to fig. 1, a method for preparing a bacteriophage lytic enzyme source feed additive for effectively removing impurities, comprising the steps of:
s1: preparing a culture medium, namely putting MgSO47H2O, KH2PO4, naCl, beer yeast, yeast extract powder, glucose, thiamine hydrochloride, lignin powder and bran into a mixing tank, stirring and mixing to obtain the culture medium, and slowly adding the glucose and the thiamine hydrochloride when S1 is used for preparing the culture medium, and stirring and adding the glucose and the thiamine hydrochloride when the glucose and the thiamine hydrochloride are added;
s2: preparing a phage stock solution, taking a prepared culture medium as a raw material, adding calcium chloride, magnesium chloride, banana puree and active carbon for mixing, removing floating substances floating on the upper layer, preparing the phage stock solution, and adding the calcium chloride, the magnesium chloride, the banana puree and the active carbon to enable the calcium chloride, the magnesium chloride, the banana puree and the active carbon to react with each other, so that the phage stock solution is more nutritious while removing peculiar smell and impurities, and the culture efficiency is improved;
s3: preparing auxiliary materials, namely culturing bacteriophage firstly, preparing a production strain from the bacteriophage, performing lysis treatment on the production strain, collecting lysate for later use, performing sterilization treatment on a bacteriophage stock solution after induction, and performing treatment for later use, wherein an induction buffer solution is added during induction for induction, and the addition amount of the induction buffer solution is 180mol/L Na2HPO4, 130mmol/L CaCl2 and 100mmol/L MgCl2;
s4: expanding culture fermentation, namely taking a bacteriophage lysate and a bacteriophage stock solution after induced sterilization as raw materials, performing inclined plane expanding culture treatment on strains, adding the bacteriophage stock solution during expanding culture, then putting the bacteriophage stock solution into eggplant bottle seeds for expanding culture treatment, putting the treated bacteriophage stock solution into a seed fermentation tank for fermentation treatment, putting the strains into the fermentation tank again for secondary fermentation after fermentation, heating the strains at the heating temperature of 36 ℃ during expanding culture in S4, and then performing intermittent stirring treatment, wherein the yield can be greatly improved through the secondary expanding culture treatment, and the secondary fermentation treatment is performed, so that the problem of large investment and low production efficiency of single-strain fermentation is solved;
s5: impurity removal, namely filtering and impurity removal treatment is carried out when phage lysate and induced sterilized phage stock solution are added each time during propagation fermentation, the addition amount is controlled, filtering treatment is carried out through a common filtering gauze during impurity removal in S5, filtered impurities are collected for later use, impurities added into raw materials can be controlled through impurity removal treatment, the impurities are prevented from influencing culture and fermentation of a culture medium, and the impurity removal effect is improved;
s6: centrifuging and recovering, performing centrifugal treatment on the secondary fermentation solution after the secondary fermentation, filtering the solution by adopting a microfiltration and ultrafiltration purification concentration membrane during centrifugation, then collecting thalli, recovering the centrifugal liquid left by the thalli, taking the recovered liquid as phage stock solution for later use, performing secondary filtration on the centrifugal liquid during recovery in S6 to remove large-particle impurities, and filtering by adopting the microfiltration and ultrafiltration purification concentration membrane, so that impurities can be more effectively removed, the complete structure of the phage can be preserved, and not only can small-molecular proteins and other various nutrient substances in the solution be separated, but also the concentration of target substances can be effectively improved, and the quality guarantee period can be prolonged;
s7: purifying and concentrating, namely purifying and concentrating the thalli collected after centrifugation, concentrating the live bacterial sludge, effectively removing harmful impurities through the processes of fermentation, impurity removal, purification and concentration, improving the content of a target object, keeping the biological activity for a long time, and effectively reducing the production cost while improving the production efficiency;
s8: mixing adsorption, then mixing adsorption, adopting specific carrier gel for matching adsorption during adsorption, and adopting specific carrier gel for adsorption, thereby solving the problem that bacteriophage is easy to inactivate, and prolonging the storage life of bacteriophage;
s9: drying and crushing, namely putting the adsorbed material into a drying chamber for drying, crushing after drying, and filtering, removing impurities and crushing for multiple times while crushing to obtain a feed additive finished product.
Example 2
Referring to fig. 1, a method for preparing a bacteriophage lytic enzyme source feed additive for effectively removing impurities, comprising the steps of:
s1: preparing a culture medium, namely putting MgSO47H2O, KH2PO4, naCl, beer yeast, yeast extract powder, glucose, thiamine hydrochloride, lignin powder and bran into a mixing tank, stirring and mixing to obtain the culture medium, and slowly adding glucose and thiamine hydrochloride when S1 is used for preparing the culture medium, and stirring and adding the glucose and thiamine hydrochloride when the glucose and the thiamine hydrochloride are added;
s2: preparing a phage stock solution, taking a prepared culture medium as a raw material, adding calcium chloride, magnesium chloride, banana puree and active carbon for mixing, fishing out floating substances floating on the upper layer, preparing the phage stock solution, adding BSA, lentinan, ganoderan and tea polyphenol for mixing in S2 during preparing the phage stock solution, and enabling the materials to react with each other through the addition of the calcium chloride, the magnesium chloride, the banana puree and the active carbon, so that the materials are more nutritious while removing peculiar smell and impurities, and the culture efficiency is improved;
s3: preparing auxiliary materials, namely culturing bacteriophage firstly, preparing a production strain from the bacteriophage, performing lysis treatment on the production strain, collecting lysate for later use, performing sterilization treatment on a bacteriophage stock solution after induction, and performing treatment for later use, wherein an induction buffer solution is added during induction for induction, and the addition amount of the induction buffer solution is 110mol/L Na2HPO4, 125mmol/L CaCl2 and 70mmol/L MgCl2;
s4: performing amplification culture fermentation, namely taking a bacteriophage lysate and a bacteriophage stock solution after induced sterilization as raw materials, performing inclined plane amplification culture treatment on strains, adding the bacteriophage stock solution during amplification culture, then putting the bacteriophage stock solution into eggplant bottle seeds for amplification culture treatment, putting the treated bacteriophage stock solution into a seed fermentation tank for fermentation treatment, and putting the strains into the fermentation tank again for secondary fermentation after fermentation, wherein the yield can be greatly improved through secondary amplification culture treatment, and the secondary fermentation treatment is performed, and the problem of large investment and low production efficiency in single-strain fermentation is solved through a multi-strain fermentation process in the same fermentation tank;
s5: impurity removal, wherein during culture expanding fermentation, filtering and impurity removal treatment are carried out each time when phage lysate and phage stock solution after induced sterilization are added, the addition amount is controlled, impurities added into raw materials can be controlled through impurity removal treatment, the impurities are prevented from influencing culture and fermentation of a culture medium, and the impurity removal effect is improved;
s6: centrifuging and recycling, centrifuging the secondary fermented product, filtering the secondary fermented product by adopting a purification and concentration membrane with microfiltration and ultrafiltration during centrifuging, collecting thalli, recycling the centrifugal liquid left by the thalli, taking the recycled liquid as phage stock solution for later use, and filtering the secondary fermented product by adopting the purification and concentration membrane with microfiltration and ultrafiltration, so that impurities can be removed more effectively, the complete structure of the phage can be stored, small molecular proteins and other various nutrient substances in the solution can be separated, and the concentration of a target substance can be effectively improved, and the quality guarantee period can be prolonged;
s7: purifying and concentrating, namely purifying and concentrating the thalli collected after centrifugation to concentrate the live bacterial sludge, and effectively removing harmful impurities to improve the content of a target object and keep the biological activity for a long time through the processes of fermentation, impurity removal, purification and concentration, so that the production cost is effectively reduced while the production efficiency is improved;
s8: mixing and adsorbing, then mixing and adsorbing, adopting the special carrier gel for matching and adsorbing during the adsorption, and adopting the special carrier gel for adsorption, thereby solving the problem that the bacteriophage is easy to inactivate and prolonging the storage life of the bacteriophage;
s9: drying and crushing, namely putting the adsorbed material into a drying chamber for drying, crushing after drying, and filtering, removing impurities and crushing for multiple times while crushing to obtain a feed additive finished product.
In the description herein, it is noted that relational terms such as first and second, and the like, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A preparation method of a phage lyase source feed additive capable of effectively removing impurities is characterized by comprising the following steps:
s1: preparing culture medium, putting MgSO47H2O, KH2PO4, naCl, beer yeast, yeast extract powder, glucose, thiamine hydrochloride, lignin powder and bran into a mixing tank, stirring and mixing to obtain culture medium;
s2: preparing a bacteriophage stock solution, taking the prepared culture medium as a raw material, adding calcium chloride, magnesium chloride, banana mud and active carbon for mixing, and fishing out the floating substances floating on the upper layer to prepare the bacteriophage stock solution;
s3: preparing auxiliary materials, namely culturing bacteriophage firstly, forming a production strain by the bacteriophage, carrying out cracking treatment on the production strain, collecting a cracking solution for later use, carrying out sterilization treatment on a bacteriophage stock solution after induction, treating the bacteriophage stock solution for later use, adding an induction buffer solution for induction during induction, wherein the addition amount of the induction buffer solution is 110-180mol/L Na2HPO4, 120-200mmol/L CaCl2 and 66-150mmol/L MgCl2;
s4: performing propagation fermentation, namely performing inclined plane propagation treatment on strains by using bacteriophage lysate and bacteriophage stock solution subjected to induced sterilization as raw materials, adding the bacteriophage stock solution during propagation, then putting the strains into eggplant bottle seeds for propagation treatment, putting the strains into a seed fermentation tank for fermentation treatment after treatment, and putting the strains into the fermentation tank for secondary fermentation after fermentation;
s5: removing impurities, namely filtering and removing impurities when phage lysate and phage stock solution after induced sterilization are added each time during propagation fermentation, and controlling the addition amount;
s6: centrifuging and recovering, centrifuging after secondary fermentation, filtering with a purification and concentration membrane of microfiltration and ultrafiltration during centrifugation, collecting thallus, recovering the residual centrifugate of the thallus, and using the recovered liquid as bacteriophage stock solution for later use;
s7: purifying and concentrating, namely purifying and concentrating the thalli collected after centrifugation, and concentrating the live bacterial sludge;
s8: mixing and adsorbing, and then performing mixing and adsorbing, wherein a specific carrier gel is adopted for matching and adsorbing during adsorbing;
s9: drying and crushing, namely putting the adsorbed material into a drying chamber for drying, crushing after drying, and filtering, impurity removing and crushing for multiple times while crushing to obtain a feed additive finished product.
2. The method for preparing a phage lyase-derived feed additive effective in removing impurities as claimed in claim 1, wherein the step of S1 is carried out by adding glucose and thiamine hydrochloride slowly during the preparation of the culture medium while stirring.
3. The method for preparing a phage lytic enzyme source feed additive capable of effectively removing impurities according to claim 2, wherein BSA, lentinan, ganoderan, and tea polyphenol are added and mixed in S2 during preparation of phage stock solution.
4. The method for preparing a feed additive derived from a bacteriophage lytic enzyme capable of effectively removing impurities according to claim 1, wherein the S4 is heated at 30-40 ℃ during propagation and then subjected to intermittent stirring.
5. The method for preparing a phage lyase-derived feed additive capable of effectively removing impurities as claimed in claim 4, wherein the step of S5 is carried out by filtering with a common filter gauze during impurity removal, and the filtered impurities are collected for further use.
6. The method of claim 1, wherein the step of recovering the centrifugate in S6 is performed by filtering again to remove large particle impurities.
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