CN115137876A - Hectorite bioceramic artificial bone and preparation method thereof - Google Patents
Hectorite bioceramic artificial bone and preparation method thereof Download PDFInfo
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- CN115137876A CN115137876A CN202210737754.XA CN202210737754A CN115137876A CN 115137876 A CN115137876 A CN 115137876A CN 202210737754 A CN202210737754 A CN 202210737754A CN 115137876 A CN115137876 A CN 115137876A
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- hectorite
- artificial bone
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- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 122
- KWLMIXQRALPRBC-UHFFFAOYSA-L hectorite Chemical compound [Li+].[OH-].[OH-].[Na+].[Mg+2].O1[Si]2([O-])O[Si]1([O-])O[Si]([O-])(O1)O[Si]1([O-])O2 KWLMIXQRALPRBC-UHFFFAOYSA-L 0.000 title claims abstract description 56
- 229910000271 hectorite Inorganic materials 0.000 title claims abstract description 56
- 239000003462 bioceramic Substances 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 238000007639 printing Methods 0.000 claims abstract description 81
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 42
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 22
- 238000010146 3D printing Methods 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 239000007864 aqueous solution Substances 0.000 claims description 27
- 239000008213 purified water Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 22
- 238000005245 sintering Methods 0.000 claims description 21
- 238000011068 loading method Methods 0.000 claims description 17
- 238000011049 filling Methods 0.000 claims description 16
- 239000011148 porous material Substances 0.000 claims description 14
- 230000007547 defect Effects 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 12
- 239000000919 ceramic Substances 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 11
- XCOBTUNSZUJCDH-UHFFFAOYSA-B lithium magnesium sodium silicate Chemical compound [Li+].[Li+].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Na+].[Na+].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].[Mg+2].O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3.O1[Si](O2)([O-])O[Si]3([O-])O[Si]1([O-])O[Si]2([O-])O3 XCOBTUNSZUJCDH-UHFFFAOYSA-B 0.000 claims description 9
- 229940094522 laponite Drugs 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 7
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 7
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 7
- 239000000378 calcium silicate Substances 0.000 claims description 5
- 229910052918 calcium silicate Inorganic materials 0.000 claims description 5
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 3
- 230000008439 repair process Effects 0.000 abstract description 3
- 206010065687 Bone loss Diseases 0.000 abstract description 2
- 206010061363 Skeletal injury Diseases 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 230000002188 osteogenic effect Effects 0.000 abstract description 2
- 230000017423 tissue regeneration Effects 0.000 abstract description 2
- 238000005303 weighing Methods 0.000 description 18
- 239000000203 mixture Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 6
- 210000003484 anatomy Anatomy 0.000 description 5
- 238000007373 indentation Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 238000011960 computer-aided design Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000010603 microCT Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009818 osteogenic differentiation Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 206010052428 Wound Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000012867 bioactive agent Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000010478 bone regeneration Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- IPGANOYOHAODGA-UHFFFAOYSA-N dilithium;dimagnesium;dioxido(oxo)silane Chemical compound [Li+].[Li+].[Mg+2].[Mg+2].[O-][Si]([O-])=O.[O-][Si]([O-])=O.[O-][Si]([O-])=O IPGANOYOHAODGA-UHFFFAOYSA-N 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 208000028774 intestinal disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000012802 nanoclay Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- 230000004819 osteoinduction Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000013500 performance material Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
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- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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Abstract
The invention discloses a hectorite bioceramic artificial bone and a preparation method thereof, wherein hectorite and bioceramic are used as raw materials and are mixed with polyvinyl alcohol to prepare paste with good printability, a host bone prototype model is obtained to design an artificial bone three-dimensional model, a bioceramic 3D printer is used for carrying out non-silk 3D printing, and the printed artificial bone is sintered to prepare the hectorite bioceramic artificial bone. The method is simple in forming, the used raw materials are low in cost and have good biocompatibility, the prepared hectorite bioceramic artificial bone has osteogenic inductivity, good bone tissue repair capacity and good mechanical strength, printing of the personalized artificial bone is achieved through a digital design technology, and the hectorite bioceramic artificial bone is beneficial to clinically applying to repair of human bone injury or bone loss.
Description
Technical Field
The invention belongs to the field of bone repair, and particularly relates to a hectorite bioceramic artificial bone and a preparation method thereof.
Background
The hectorite is an artificially synthesized nano clay, is a layered silicate material, is also called lithium magnesium silicate/lithium magnesium sodium silicate, is composed of layered disc nanoparticles with the diameter of about 25nm and the thickness of 1nm, and has wide application prospect in the field of nano biological materials due to the advantages of low price, excellent performance, high safety and the like. Clay has been used in many ways as a bioactive agent for the treatment of wounds, hemostasis, intestinal diseases, skin diseases, etc., and also as a stabilizer, thickener, etc. for other liquids due to its structural diversity.
Laponite exhibits good biocompatibility in interaction with cells and can regulate proliferation and differentiation of cells, so that laponite is widely used in the fields of tissue engineering, wound healing, bioprinting and the like, and is one of novel, widely-used and good-performance materials in the field of regenerative medicine. The use of laponite in bone tissue engineering to promote bone regeneration has been a popular study in the field of materials science in recent years. Some studies have demonstrated that laponite has biological activity, and even in the absence of growth factors, laponite induces osteogenic differentiation of various cells such as osteogenic precursor cells, human mesenchymal stem cells, human adipose-derived cells, and the like. Laponite dissociates into individual particles of lithium, magnesium, silicon, etc., which cause upregulation of osteogenesis-related genes and pathways, thereby inducing osteogenic differentiation of cells.
With the accelerated development of 3D printing, more and more materials are used for research. Whereas the most used in the field of bone repair is the silkless 3D printing technique. However, due to the characteristics of hectorite, the paste which meets the silk-free 3D printing is prepared and belongs to the gel class, so that the mechanical property of the printed artificial bone is very low, and even the shape of the artificial bone cannot be maintained, and a method for enhancing the mechanical strength of the hectorite artificial bone is urgently needed.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a hectorite bioceramic artificial bone and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme to realize the purpose:
a hectorite bioceramic artificial bone is prepared from hectorite and bioceramic by a silk-free 3D printing process.
Further, the bioceramic comprises one or more of hydroxyapatite, beta-tricalcium phosphate and calcium silicate.
A preparation method of a hectorite bioceramic artificial bone comprises the following steps:
dissolving polyvinyl alcohol in purified water to prepare a polyvinyl alcohol aqueous solution;
adding purified water into printing powder of hectorite and biological ceramics, uniformly stirring, adding a polyvinyl alcohol aqueous solution, uniformly stirring again, and filling into a charging barrel for defoaming to obtain a printing paste body;
acquiring CT/MRI/X rays of a defect part of a host bone, processing the data, acquiring a prototype model of the host bone, and designing a suitable three-dimensional model of the artificial bone;
loading the printing paste into a printing head of a 3D printer, loading the designed three-dimensional model into 3D printing software, setting printing parameters, and starting the 3D printer to finish printing of the artificial bone;
and sintering the printed artificial bone to obtain the hectorite bioceramic artificial bone product.
Further, the mass fraction of the polyvinyl alcohol aqueous solution is 4-10%.
Furthermore, the mass ratio of the hectorite to the bioceramic in the printing paste is 1:1-3, and the added purified water is 2-4 times of the mass of the hectorite and the bioceramic printing powder.
Furthermore, the polyvinyl alcohol aqueous solution added into the printing paste is 1/6-1/4 of the sum of the mass of the laponite, the bioceramic and the purified water.
Further, the data are processed by a software 3D Slicer to obtain a host bone prototype model, a suitable artificial bone three-dimensional model is designed by Free CAD software, the model is stored as an STL format file and loaded into PC Printer software, and the silk-Free 3D printing is carried out by a biological ceramic 3D Printer.
Further, the printing parameters are set to: the filling rate is 40-80%, the printing layer height is 0.1-0.3 mm, the average filament diameter is 500-900 μm, and the average pore diameter is 300-800 μm.
Furthermore, in the sintering process, the sintering temperature is 840-880 ℃, and the heat preservation time is 2-4 h.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a hectorite bioceramic artificial bone and a preparation method thereof. The method is simple in forming, printing of the personalized artificial bone can be achieved through a digital design technology, the used raw materials are low in cost and have good biocompatibility, the prepared hectorite bioceramic artificial bone has osteoinduction, good bone tissue repair capacity and good mechanical strength, the hectorite bioceramic artificial bone is favorable for clinically repairing bone injury or bone loss of a human body, and the application prospect is very wide.
Drawings
In order to more clearly explain the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a 3D printed image of a hectorite bioceramic artificial bone prepared in example 1 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention is described in further detail below with reference to the accompanying drawings:
the invention provides a preparation method of a hectorite bioceramic artificial bone, which comprises the following steps:
preparing a polyvinyl alcohol aqueous solution: dissolving polyvinyl alcohol in purified water to obtain a polyvinyl alcohol solution with the mass fraction of 4% -10%;
preparing a printing paste: weighing hectorite and biological ceramic in a mass ratio of 1:1-3, uniformly stirring the hectorite and the biological ceramic to obtain printing powder, weighing purified water in an amount which is 2-4 times of the printing powder, uniformly stirring the purified water, weighing polyvinyl alcohol aqueous solution which is 1/6-1/4 of the sum of the printing powder and the purified water, uniformly stirring the polyvinyl alcohol aqueous solution again, filling the mixture into a charging barrel, and defoaming the mixture to obtain paste with good printability.
Artificial bone three-dimensional model: acquiring CT/MRI/X rays of a defect part of a host bone, processing data by using software 3D Slicer 4.10.2, acquiring a prototype model of the host bone, and designing an STL (synthetic bone template) file of an applicable artificial bone three-dimensional model by using Free CAD (computer aided design) software;
silkless 3D printing: the artificial bone is printed by a biological ceramic printer. Firstly, loading the uniformly mixed printing paste into a printing head, then loading the designed STL file of the artificial bone three-dimensional model into PC Printer software, and setting the printing process parameters as follows: the filling rate is 40-80%, the printing layer height is 0.1-0.3 mm, the average filament diameter is 500-900 microns, the average pore diameter is 300-800 microns, the printing paste is uniformly extruded through a screw propeller at a constant speed, a workbench performs synthetic motion along an x-y axis, a printing head moves along a z axis, and the printing is sequentially performed layer by layer, so that the printing of the artificial bone is finally completed;
and (3) sintering: and (3) preserving the heat of the printed artificial bone at 840-880 ℃ for 6-8 h, and sintering to obtain the hectorite bioceramic artificial bone.
Example 1:
preparing a polyvinyl alcohol aqueous solution: dissolving 10g of polyvinyl alcohol in 90g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 10%;
preparing a printing paste body: weighing 10g of hectorite and 10g of hydroxyapatite, uniformly stirring the hectorite and the hydroxyapatite to obtain printing powder, weighing 40g of purified water, uniformly stirring the purified water, weighing 10g of polyvinyl alcohol aqueous solution, uniformly stirring the polyvinyl alcohol aqueous solution again, filling the polyvinyl alcohol aqueous solution into a charging barrel, and defoaming the polyvinyl alcohol aqueous solution to obtain paste with good printability;
artificial bone three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer 4.10.2, acquiring a host bone prototype model, and designing an artificial bone three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
silkless 3D printing: and printing the artificial bone by using a biological ceramic printer. Firstly, loading the uniformly mixed printing paste into a printing head, then loading the designed STL file of the artificial bone three-dimensional model into PC Printer software, and setting the printing process parameters as follows: the filling rate is 45%, the printing layer height is 0.1mm, the average filament diameter is 600 microns, the average pore diameter is 500 microns, the printing paste is uniformly extruded at a constant speed through a spiral propeller, a workbench performs synthetic motion along an x-y axis, a printing head moves along a z axis, and the artificial bone is printed layer by layer in sequence, and finally the printing of the artificial bone is completed;
and (3) sintering: and sintering the printed artificial bone at the sintering temperature of 840 ℃ for 4h to obtain the hectorite bioceramic artificial bone as shown in figure 1.
Example 2:
preparing a polyvinyl alcohol aqueous solution: dissolving 8g of polyvinyl alcohol in 92g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 8%;
preparing a printing paste: weighing 10g of hectorite and 15g of beta-tricalcium phosphate, uniformly stirring the hectorite and the beta-tricalcium phosphate to obtain printing powder, weighing 62.5g of purified water, uniformly stirring the purified water, weighing 15.91g of polyvinyl alcohol aqueous solution, uniformly stirring the polyvinyl alcohol aqueous solution again, filling the polyvinyl alcohol aqueous solution into a charging barrel, and defoaming the polyvinyl alcohol aqueous solution to obtain paste with good printability;
artificial bone three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer 4.10.2, acquiring a host bone prototype model, and designing an artificial bone three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
silkless 3D printing: the artificial bone is printed by a biological ceramic printer. Firstly, loading the uniformly mixed printing paste into a printing head, then loading the designed STL file of the artificial bone three-dimensional model into PC Printer software, and setting the printing process parameters as follows: the filling rate is 40%, the printing layer height is 0.15mm, the average filament diameter is 500 microns, the average pore diameter is 300 microns, the printing paste is uniformly extruded at a constant speed through a spiral propeller, a workbench performs synthetic motion along an x-y axis, a printing head moves along a z axis, and the artificial bone is printed layer by layer in sequence, and finally the printing of the artificial bone is completed;
and (3) sintering: and sintering the printed artificial bone at 850 ℃ for 3.5 hours to obtain the hectorite bioceramic artificial bone after sintering.
Example 3:
preparing a polyvinyl alcohol aqueous solution: dissolving 6g of polyvinyl alcohol in 94g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 6%;
preparing a printing paste body: weighing 10g of hectorite, 8g of hydroxyapatite, 6g of beta-tricalcium phosphate and 6g of calcium silicate, uniformly stirring the mixture to obtain printing powder, weighing 60g of purified water, uniformly stirring the mixture, weighing 18g of polyvinyl alcohol aqueous solution, uniformly stirring the mixture again, filling the mixture into a charging barrel, and defoaming the mixture to obtain paste with good printability;
artificial bone three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer 4.10.2, acquiring a host bone prototype model, and designing an artificial bone three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
silkless 3D printing: the artificial bone is printed by a biological ceramic printer. Firstly, loading the uniformly mixed printing paste into a printing head, then loading the designed STL file of the artificial bone three-dimensional model into PC Printer software, and setting the printing process parameters as follows: the filling rate is 50%, the printing layer height is 0.2mm, the average filament diameter is 700 mu m, the average pore diameter is 400 mu m, the printing paste is uniformly extruded at a constant speed through a spiral propeller, the workbench performs synthetic motion along an x-y axis, the printing head moves along a z axis, and the artificial bone is printed layer by layer in sequence, and finally the printing of the artificial bone is completed;
and (3) sintering: and sintering the printed artificial bone at 860 ℃ for 3h to obtain the hectorite bioceramic artificial bone.
Example 4:
preparing a polyvinyl alcohol aqueous solution: dissolving 5g of polyvinyl alcohol in 95g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 5%;
preparing a printing paste: weighing 10g of hectorite, 11g of hydroxyapatite, 7g of beta-tricalcium phosphate and 7g of calcium silicate, uniformly stirring the mixture to obtain printing powder, weighing 122.5g of purified water, uniformly stirring the mixture, weighing 35g of polyvinyl alcohol aqueous solution, uniformly stirring the mixture again, filling the mixture into a charging barrel, and defoaming the mixture to obtain paste with good printability;
artificial bone three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer 4.10.2, acquiring a host bone prototype model, and designing an artificial bone three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
silkless 3D printing: the artificial bone is printed by a biological ceramic printer. Firstly, loading the uniformly mixed printing paste into a printing head, then loading the designed STL file of the artificial bone three-dimensional model into PC Printer software, and setting the printing process parameters as follows: the filling rate is 60%, the printing layer height is 0.25mm, the average filament diameter is 800 microns, the average pore diameter is 600 microns, the printing paste is uniformly extruded at a constant speed through a spiral propeller, a workbench performs synthetic motion along an x-y axis, a printing head moves along a z axis, and the artificial bone is printed layer by layer in sequence to finally finish printing of the artificial bone;
and (3) sintering: and sintering the printed artificial bone at 870 ℃ for 2.5h to obtain the hectorite bioceramic artificial bone.
Example 5:
preparing a polyvinyl alcohol aqueous solution: dissolving 4g of polyvinyl alcohol in 96g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 4%;
preparing a printing paste: weighing 10g of hectorite, 12g of hydroxyapatite, 9g of beta-tricalcium phosphate and 9g of calcium silicate, uniformly stirring the mixture to obtain printing powder, weighing 160g of purified water, uniformly stirring the mixture, weighing 33.33g of polyvinyl alcohol aqueous solution, uniformly stirring the mixture again, filling the mixture into a charging barrel, and defoaming the mixture to obtain paste with good printability;
artificial bone three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer 4.10.2, acquiring a host bone prototype model, and designing an artificial bone three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
silkless 3D printing: and printing the artificial bone by using a biological ceramic printer. Firstly, loading the uniformly mixed printing paste into a printing head, then loading the designed STL file of the artificial bone three-dimensional model into PC Printer software, and setting the printing process parameters as follows: the filling rate is 80%, the printing layer height is 0.3mm, the average filament diameter is 900 microns, the average pore diameter is 800 microns, the printing paste is uniformly extruded at a constant speed through a spiral propeller, a workbench performs synthetic motion along an x-y axis, a printing head moves along a z axis, and the artificial bone is printed layer by layer in sequence, and finally the printing of the artificial bone is completed;
and (3) sintering: and sintering the printed artificial bone at 880 ℃, keeping the temperature for 2 hours, and obtaining the hectorite bioceramic artificial bone after sintering.
And (3) testing the performance of the hectorite bioceramic artificial bone:
the porosity, average filament diameter, average pore diameter and ball indentation strength of the hectorite bioceramic artificial bone prepared in example 1 are tested by the following specific test methods: the porosity, the average silk diameter, the average pore diameter and the pore connectivity refer to a porous metal material X-ray CT detection method for a surgical implant of GB/T36984-2018, a sample is subjected to Micro-CT detection, and three-dimensional reconstruction is carried out on all scanned faults to obtain a sample three-dimensional body model; the ratio of the pore volume to the total sample volume was then calculated as the three-dimensional porosity. And analyzing the image of the Micro-CT, calculating the diameter of the aperture wire, describing the aperture of the circular hole by adopting the diameter of the circular hole, describing the aperture of the slit hole by adopting the distance between two pairs of walls, measuring 12 points on each surface, and taking the average value as the average aperture and the average wire diameter. The ball indentation strength is referred to a ball indentation test method in a YY/T1558.3-2017 material mechanical strength detection method, ten printed samples are taken, the samples are cubes with the length, the width and the height of 10mm, a universal testing machine is used for testing at the loading speed of 0.5mm/min, the maximum load in the testing process is recorded, and the average value of the maximum load is used as the final ball indentation strength. The result shows that the porosity is 46 percent, the average wire diameter is 600 mu m, the average pore diameter is 500 mu m, the ball indentation strength is 152N, and the result shows that the hectorite bioceramic artificial bone has high porosity, uniform wire diameter and pore diameter and highly uniform structure, is very beneficial to cell attachment and drug release, has higher strength, and can ensure the stability of the artificial bone during implantation.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The hectorite bioceramic artificial bone is characterized in that hectorite and bioceramic are used as raw materials and are prepared through a filament-free 3D printing process.
2. The laponite bioceramic artificial bone of claim 1, wherein the bioceramic comprises one or more of hydroxyapatite, β -tricalcium phosphate, calcium silicate.
3. A method for preparing a hectorite bioceramic artificial bone according to any one of claims 1-2, comprising:
dissolving polyvinyl alcohol in purified water to prepare a polyvinyl alcohol aqueous solution;
adding purified water into printing powder of hectorite and biological ceramics, uniformly stirring, adding a polyvinyl alcohol aqueous solution, uniformly stirring again, and filling into a charging barrel for defoaming to obtain a printing paste body;
acquiring CT/MRI/X rays of a defect part of a host bone, processing the data, acquiring a prototype model of the host bone, and designing a suitable three-dimensional model of the artificial bone;
loading the printing paste into a printing head of a 3D printer, loading the designed three-dimensional model into 3D printing software, setting printing parameters, and starting the 3D printer to finish printing of the artificial bone;
and sintering the printed artificial bone to obtain the hectorite bioceramic artificial bone product.
4. The method for preparing the hectorite bioceramic artificial bone according to claim 3, wherein the mass fraction of the polyvinyl alcohol aqueous solution is 4-10%.
5. The method for preparing a hectorite bioceramic artificial bone according to claim 3, wherein the mass ratio of hectorite to bioceramic in the printed paste is 1:1-3, and the added purified water is 2-4 times of the mass of hectorite and bioceramic printed powder.
6. The method for preparing a hectorite bioceramic artificial bone according to claim 3, wherein the polyvinyl alcohol aqueous solution added into the paste for printing is 1/6 to 1/4 of the sum of the mass of the hectorite, the bioceramic and the purified water.
7. The method for preparing a hectorite bioceramic artificial bone according to claim 3, wherein a host bone prototype model is obtained after data is processed by a 3D Slicer software, an applicable artificial bone three-dimensional model is designed by Free CAD software, the model is stored as an STL format file and loaded into a PC Printer software, and the bioceramic 3D Printer is used for 3D printing without silk.
8. The method for preparing a hectorite bioceramic artificial bone according to claim 3, wherein printing parameters are set as follows: the filling rate is 40-80%, the printing layer height is 0.1-0.3 mm, the average filament diameter is 500-900 μm, and the average pore diameter is 300-800 μm.
9. The method for preparing a hectorite bioceramic artificial bone according to claim 3, wherein the sintering temperature is 840-880 ℃ and the heat preservation time is 2-4 h in the sintering process.
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Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104163622A (en) * | 2014-06-06 | 2014-11-26 | 上海交通大学附属第一人民医院 | Preparation method and application of laponite bioceramics |
CN104325644A (en) * | 2014-10-20 | 2015-02-04 | 西安点云先进材料科技有限公司 | Filament-free three-dimensional printing method |
CN109180175A (en) * | 2018-10-25 | 2019-01-11 | 河北大洲智造科技有限公司 | A kind of photocuring 3D printing bioceramic slurry and preparation method thereof, bone tissue engineering scaffold and its application |
CN110075359A (en) * | 2019-03-26 | 2019-08-02 | 华南理工大学 | A kind of ultrasonic wave added prepares porous bone cement bracket and preparation method thereof |
CN110075349A (en) * | 2019-04-09 | 2019-08-02 | 温州医科大学 | A kind of bioactivity glass compound rest and application |
CN110357657A (en) * | 2019-08-15 | 2019-10-22 | 河北大洲智造科技有限公司 | A kind of 3D printing bioceramic slurry and preparation method thereof, a kind of bio-ceramic artificial bone and preparation method thereof |
CN110665063A (en) * | 2019-10-28 | 2020-01-10 | 中国人民解放军第四军医大学 | 3D biological printing ink and preparation method thereof, tissue engineering scaffold and preparation method thereof |
CN110721336A (en) * | 2019-11-26 | 2020-01-24 | 许雄程 | Nano lithium magnesium silicate/polycaprolactone composite material and preparation method thereof |
CN111012947A (en) * | 2019-12-30 | 2020-04-17 | 南京财经大学 | Injectable and self-healing starch-based hydrogel and preparation method and application thereof |
CN111070376A (en) * | 2019-12-25 | 2020-04-28 | 西安点云生物科技有限公司 | 3D printing bionic porous bioceramic artificial bone and preparation method thereof |
CN112960988A (en) * | 2021-02-02 | 2021-06-15 | 烟台正海生物科技股份有限公司 | 3D printing cuttable biological ceramic support and preparation method and application thereof |
-
2022
- 2022-06-27 CN CN202210737754.XA patent/CN115137876A/en active Pending
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104163622A (en) * | 2014-06-06 | 2014-11-26 | 上海交通大学附属第一人民医院 | Preparation method and application of laponite bioceramics |
CN104325644A (en) * | 2014-10-20 | 2015-02-04 | 西安点云先进材料科技有限公司 | Filament-free three-dimensional printing method |
CN109180175A (en) * | 2018-10-25 | 2019-01-11 | 河北大洲智造科技有限公司 | A kind of photocuring 3D printing bioceramic slurry and preparation method thereof, bone tissue engineering scaffold and its application |
CN110075359A (en) * | 2019-03-26 | 2019-08-02 | 华南理工大学 | A kind of ultrasonic wave added prepares porous bone cement bracket and preparation method thereof |
CN110075349A (en) * | 2019-04-09 | 2019-08-02 | 温州医科大学 | A kind of bioactivity glass compound rest and application |
CN110357657A (en) * | 2019-08-15 | 2019-10-22 | 河北大洲智造科技有限公司 | A kind of 3D printing bioceramic slurry and preparation method thereof, a kind of bio-ceramic artificial bone and preparation method thereof |
CN110665063A (en) * | 2019-10-28 | 2020-01-10 | 中国人民解放军第四军医大学 | 3D biological printing ink and preparation method thereof, tissue engineering scaffold and preparation method thereof |
CN110721336A (en) * | 2019-11-26 | 2020-01-24 | 许雄程 | Nano lithium magnesium silicate/polycaprolactone composite material and preparation method thereof |
CN111070376A (en) * | 2019-12-25 | 2020-04-28 | 西安点云生物科技有限公司 | 3D printing bionic porous bioceramic artificial bone and preparation method thereof |
CN111012947A (en) * | 2019-12-30 | 2020-04-17 | 南京财经大学 | Injectable and self-healing starch-based hydrogel and preparation method and application thereof |
CN112960988A (en) * | 2021-02-02 | 2021-06-15 | 烟台正海生物科技股份有限公司 | 3D printing cuttable biological ceramic support and preparation method and application thereof |
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