CN115054725B - Hectorite 3D printing artificial bone scaffold and preparation method thereof - Google Patents
Hectorite 3D printing artificial bone scaffold and preparation method thereof Download PDFInfo
- Publication number
- CN115054725B CN115054725B CN202210736957.7A CN202210736957A CN115054725B CN 115054725 B CN115054725 B CN 115054725B CN 202210736957 A CN202210736957 A CN 202210736957A CN 115054725 B CN115054725 B CN 115054725B
- Authority
- CN
- China
- Prior art keywords
- printing
- hectorite
- artificial bone
- bone scaffold
- scaffold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y70/00—Materials specially adapted for additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y80/00—Products made by additive manufacturing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/25—Process efficiency
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Dermatology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a 3D printing artificial bone scaffold of hectorite and a preparation method thereof, wherein the 3D printing artificial bone scaffold of hectorite and purified water are prepared into printable paste, a three-dimensional model of the artificial bone scaffold meeting the requirements is designed after a host bone prototype model is obtained according to medical image data of a host, the designed three-dimensional model of the artificial bone scaffold is printed layer by using a bioceramic printer through a wire-free 3D printing, the printed scaffold is subjected to crosslinking and sintering to obtain the 3D printing artificial bone scaffold of the hectorite, and the 3D printing artificial bone scaffold of the hectorite prepared by the method has the antibacterial property of the hectorite, the capability of promoting the bone and accelerating the wound healing, has an adjustable pore structure and porosity, has certain strength, is low in raw material cost and simple in molding, can be used for drug-carrying scaffolds for drug release, cell-carrying scaffolds for biological 3D printing and filling scaffolds for bone implantation, and has a very wide application prospect.
Description
Technical Field
The invention belongs to the field of bone repair, and particularly relates to a hectorite 3D printing artificial bone scaffold and a preparation method thereof.
Background
Hectorite is an artificially synthesized nano clay, is a layered silicate material, also called lithium magnesium silicate/lithium magnesium sodium silicate, and consists of layered disc nano particles with the diameter of about 25nm and the thickness of 1nm, and has wide application prospect in the field of nano biological materials due to the advantages of low price, excellent performance, high safety and the like. Clay has been used as a bioactive agent for treating various diseases such as wounds, hemostats, intestinal diseases, skin diseases, etc., and is also used as a stabilizer, thickener, etc. for other liquids due to its structural diversity.
The hectorite has good biocompatibility in interaction with cells and can regulate proliferation and differentiation of the cells, so that the hectorite is widely used in the fields of tissue engineering, wound healing, bioprinting and the like, and is one of novel materials with wide application and good performance in the field of regenerative medicine. The use of laponite in bone tissue engineering to promote bone regeneration is a popular study in the field of materials in recent years. Several studies have demonstrated that hectorite has biological activity and, even in the absence of growth factors, can induce osteogenic differentiation of various cells, such as osteogenic precursor cells, human bone marrow mesenchymal stem cells, human adipose-derived cells, and the like. Hectorite can dissociate into independent particles of lithium, magnesium, silicon, etc., which cause up-regulation of osteogenic related genes and pathways, thereby inducing osteogenic differentiation of cells.
However, the hectorite is used as a synthetic octahedral layered colloid material, and has extremely strong gel forming performance in a water system, so that the formation of the hectorite bracket becomes a difficult problem, and particularly, the 3D printing of the hectorite is more difficult.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a hectorite 3D printing artificial bone scaffold and a preparation method thereof.
In order to achieve the purpose, the invention is realized by adopting the following technical scheme:
a method for preparing a hectorite 3D printing artificial bone scaffold, comprising the following steps:
uniformly stirring hectorite and purified water, and filling the mixture into a charging barrel for defoaming to prepare printing paste;
acquiring CT/MRI/X rays of a host bone defect part, processing data, acquiring a host bone prototype model, and designing an applicable bracket three-dimensional model;
loading printing paste into a printing head, loading a designed three-dimensional model of the bracket into 3D printing software, setting printing parameters, starting a 3D printer, and finishing printing of the bracket;
dissolving polyvinyl alcohol in purified water to obtain a polyvinyl alcohol solution, immersing the printed scaffold in the polyvinyl alcohol solution for crosslinking to obtain the hectorite 3D printed artificial bone scaffold;
sintering the crosslinked scaffold to obtain the hectorite 3D printing artificial bone scaffold.
Further, the mass ratio of the hectorite to the purified water is 1:3-5.
Further, the data is processed by using a software 3D Slicer to obtain a host bone prototype model, an applicable bracket three-dimensional model is designed by using Free CAD software, the model is stored as an STL file and loaded into PC Printer software, and a bioceramic Printer is used for carrying out non-wire 3D printing.
Further, the mass fraction of the polyvinyl alcohol solution is 4% -10%.
Further, in the crosslinking process, the crosslinking time is 3-7 h.
Further, in the sintering process, the sintering temperature is 820-870 ℃, and the heat preservation time is 3-5 h.
The hectorite 3D printing artificial bone scaffold prepared by the preparation method is provided.
The 3D printing artificial bone scaffold of hectorite has a pore structure of 30-90 degrees and a porosity of 40-80%.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a hectorite 3D printing artificial bone scaffold and a preparation method thereof, wherein hectorite is used as a raw material, a host bone prototype model is obtained by analyzing medical image data of a host, a scaffold three-dimensional model suitable for requirements is designed, and according to the designed scaffold three-dimensional model, sintering and crosslinking are performed after printing is completed by using a non-wire 3D printing technology, so that the hectorite 3D printing artificial bone scaffold is obtained. The preparation method provides a rapid forming method of the hectorite bracket, and has the advantages of low raw material cost and simple forming; the prepared hectorite 3D printing artificial bone scaffold has osteoinductive property, antibacterial property, wound healing accelerating capability, good biocompatibility, strong bone tissue repairing capability, adjustable pore structure and porosity, certain strength, and wide application prospect, and can be used as a drug-carrying scaffold for drug release, a cell-carrying scaffold for biological 3D printing and a filling scaffold for bone implantation.
Drawings
For a clearer description of the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a flow chart of the preparation of the hectorite 3D printing artificial bone scaffold of the present invention.
Fig. 2 is a CT reconstruction of a laponite 3D printed artificial bone scaffold prepared in example 5 of the present invention.
Fig. 3 is a wire diameter measurement chart of a laponite 3D printed artificial bone scaffold prepared in example 5 of the present invention.
Fig. 4 is a ball indentation test chart of a laponite 3D printed artificial bone scaffold prepared in example 5 of the present invention.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments of the present invention. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention is described in further detail below with reference to the attached drawing figures:
referring to fig. 1, the invention provides a preparation method of a hectorite 3D printing artificial bone scaffold, comprising the following steps:
preparing a printing paste: and (3) weighing hectorite and purified water in a mass ratio of 1:3-5, uniformly stirring, and then filling into a charging barrel for defoaming to obtain paste with good printability.
And (3) a bracket three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer4.10.2 to acquire a host bone prototype model, and designing an applicable support three-dimensional model STL file by using Free CAD software;
and (3) silk-free 3D printing: and (5) carrying out silk-free 3D printing on the support by using a bioceramic printer. Firstly, filling the uniformly mixed printing paste into a printing head, then loading a designed three-dimensional model STL file of a bracket into PC Printer software, and setting parameters of a printing process as follows: filling the pore structure at 30-90 degrees, wherein the porosity is 40-80%, starting a printer, uniformly extruding the printing paste at a constant speed through a spiral propeller, enabling a workbench to perform synthetic motion along an x-y axis, enabling a printing head to move along a z axis, printing layer by layer in sequence, and finally finishing printing of the bracket;
crosslinking: dissolving polyvinyl alcohol in purified water to obtain a polyvinyl alcohol solution with the mass fraction of 4% -10%, immersing the printed hectorite bracket into the polyvinyl alcohol solution for crosslinking for 3-7 h;
sintering: and (3) preserving the heat of the crosslinked bracket for 3-5 hours at the temperature of 820-870 ℃ and obtaining the hectorite 3D printing artificial bone bracket after sintering.
Example 1:
preparing a printing paste: respectively weighing 10g of hectorite and 30g of purified water, uniformly stirring, and then filling into a charging barrel for defoaming to obtain paste with good printability.
And (3) a bracket three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer4.10.2 to acquire a host bone prototype model, and designing a support three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
and (3) silk-free 3D printing: and (5) carrying out silk-free 3D printing on the support by using a bioceramic printer. Firstly, filling the uniformly mixed printing paste into a printing head, then loading a designed three-dimensional model STL file of a bracket into PC Printer software, and setting parameters of a printing process as follows: the pore structure is filled at 30 degrees, the porosity is 80%, a printer is started, the printing paste is uniformly extruded at a constant speed through a screw propeller, a workbench moves in a combined mode along an x-y axis, a printing head moves along a z axis, printing is sequentially carried out layer by layer, and finally printing of the bracket is finished;
crosslinking: dissolving 4g of polyvinyl alcohol in 96g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 4%, and immersing the printed hectorite bracket into the polyvinyl alcohol solution for crosslinking for 7 hours;
sintering: sintering the crosslinked scaffold at 820 ℃ for 5 hours to obtain the hectorite 3D printing artificial bone scaffold.
Example 2:
preparing a printing paste: respectively weighing 10g of hectorite and 35g of purified water, uniformly stirring, and then filling into a charging barrel for defoaming to obtain paste with good printability.
And (3) a bracket three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer4.10.2 to acquire a host bone prototype model, and designing a support three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
and (3) silk-free 3D printing: and (5) carrying out silk-free 3D printing on the support by using a bioceramic printer. Firstly, filling the uniformly mixed printing paste into a printing head, then loading a designed three-dimensional model STL file of a bracket into PC Printer software, and setting parameters of a printing process as follows: the pore structure is filled at 45 degrees, the porosity is 70%, a printer is started, printing paste is uniformly extruded at a constant speed through a screw propeller, a workbench moves in a combined mode along an x-y axis, a printing head moves along a z axis, printing is sequentially carried out layer by layer, and finally printing of a bracket is completed;
crosslinking: dissolving 5g of polyvinyl alcohol in 95g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 5%, and immersing the printed hectorite bracket into the polyvinyl alcohol solution for crosslinking for 6 hours;
sintering: sintering the bracket with the crosslinked bracket at 850 ℃ for 4.5 hours; and obtaining the hectorite 3D printing artificial bone scaffold after sintering.
Example 3:
preparing a printing paste: respectively weighing 10g of hectorite and 40g of purified water, uniformly stirring, and then filling into a charging barrel for defoaming to obtain paste with good printability.
And (3) a bracket three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer4.10.2 to acquire a host bone prototype model, and designing a support three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
and (3) silk-free 3D printing: and (5) carrying out silk-free 3D printing on the support by using a bioceramic printer. Firstly, filling the uniformly mixed printing paste into a printing head, then loading a designed three-dimensional model STL file of a bracket into PC Printer software, and setting parameters of a printing process as follows: the pore structure is filled at 60 degrees, the porosity is 60 percent, a printer is started, paste is uniformly extruded through a screw propeller, a workbench moves in a combined mode along an x-y axis, a printing head moves along a z axis, printing is sequentially carried out layer by layer, and finally printing of a bracket is completed;
crosslinking: 6g of polyvinyl alcohol is dissolved in 94g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 6%, and the printed hectorite stent is immersed in the polyvinyl alcohol solution for crosslinking for 5 hours;
sintering: sintering the crosslinked bracket at 850 ℃ for 4 hours to obtain the hectorite 3D printing artificial bone bracket after sintering.
Example 4:
preparing a printing paste: respectively weighing 10g of hectorite and 45g of purified water, uniformly stirring, and then filling into a charging barrel for defoaming to obtain paste with good printability.
And (3) a bracket three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer4.10.2 to acquire a host bone prototype model, and designing a support three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
and (3) silk-free 3D printing: and (5) carrying out silk-free 3D printing on the support by using a bioceramic printer. Firstly, filling the uniformly mixed printing paste into a printing head, then loading a designed three-dimensional model STL file of a bracket into PC Printer software, and setting parameters of a printing process as follows: filling the pore structure at 75 degrees, starting a printer, uniformly extruding paste through a screw propeller at a uniform speed, enabling a workbench to perform synthetic motion along an x-y axis, enabling a printing head to move along a z axis, printing layer by layer in sequence, and finally finishing printing of a bracket;
crosslinking: 8g of polyvinyl alcohol is dissolved in 92g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 8%, and the printed hectorite stent is immersed in the polyvinyl alcohol solution for crosslinking for 4 hours;
sintering: sintering the crosslinked scaffold at 860 ℃ for 3.5 hours to obtain the hectorite 3D printing artificial bone scaffold.
Example 5:
preparing a printing paste: respectively weighing 10g of hectorite and 50g of purified water, uniformly stirring, and then filling into a charging barrel for defoaming to obtain paste with good printability.
And (3) a bracket three-dimensional model: acquiring CT/MRI/X rays of a host bone defect part, processing data by using software 3D Slicer4.10.2 to acquire a host bone prototype model, and designing a support three-dimensional model STL file suitable for a bone defect anatomical structure or special requirements by using Free CAD software;
and (3) silk-free 3D printing: and (5) carrying out silk-free 3D printing on the support by using a bioceramic printer. Firstly, filling the uniformly mixed printing paste into a printing head, then loading a designed three-dimensional model STL file of a bracket into PC Printer software, and setting parameters of a printing process as follows: setting the pore structure to be filled at 90 degrees, starting a printer, uniformly extruding paste at a uniform speed through a screw propeller, enabling a workbench to perform synthetic motion along an x-y axis, enabling a printing head to move along a z axis, printing layer by layer in sequence, and finally finishing printing of a bracket;
crosslinking: 10g of polyvinyl alcohol is dissolved in 90g of purified water to obtain a polyvinyl alcohol solution with the mass fraction of 10%, and the printed hectorite stent is immersed in the polyvinyl alcohol solution for crosslinking for 3 hours;
sintering: sintering the crosslinked bracket at 870 ℃ for 3 hours to obtain the hectorite 3D printing artificial bone bracket.
Performance test of laponite 3D printed artificial bone scaffold:
the 3D printing artificial bone scaffold prepared in example 5 was tested for porosity, average wire diameter, average pore diameter, ball indentation strength by the following specific test method: the porosity, the average wire diameter, the average pore diameter and the pore connectivity are all referred to a GB/T36984-2018 porous metal material X-ray CT detection method for surgical implants, a sample is subjected to Micro-CT detection, three-dimensional reconstruction is carried out by all faults scanned, and an obtained sample three-dimensional body model is shown in figure 2; the ratio of pore volume to total sample volume is then calculated as three-dimensional porosity. And analyzing the image of the Micro-CT, as shown in figure 3, calculating the size of the diameter of the aperture, describing the diameter of the circular hole by using the diameter of the circular hole, describing the aperture of the slit hole by using the distance between two pairs of walls, and measuring 12 points on each surface, wherein the average value is taken as the average aperture and the average diameter. Ball indentation strength reference ten printed samples were taken as ball indentation test methods in the YY/T1558.3-2017 material mechanical strength detection method, the samples were cubes with length, width and height of 10mm, and were tested at a loading speed of 0.5mm/min using a universal tester, as shown in fig. 4, and the maximum load during the test was recorded, with the average value of the maximum load as the final ball indentation strength. The result shows that the porosity is 51%, the average wire diameter is 700 mu m, the average pore diameter is 600 mu m, and the ball indentation strength is 96N, which indicates that the hectorite 3D printing artificial bone scaffold has high porosity, uniform wire diameter and pore diameter, uniform structure and high uniformity, is very favorable for cell climbing and drug release, has certain strength, and can ensure the stability of the scaffold during implantation.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The preparation method of the hectorite 3D printing artificial bone scaffold is characterized by comprising the following steps of:
uniformly stirring hectorite and purified water, and filling the mixture into a charging barrel for defoaming to prepare printing paste;
acquiring CT/MRI/X rays of a host bone defect part, processing data, acquiring a host bone prototype model, and designing an applicable bracket three-dimensional model;
loading printing paste into a printing head, loading the designed three-dimensional model of the artificial bone scaffold into 3D printing software, setting printing parameters, starting a 3D printer, and finishing printing of the artificial bone scaffold;
dissolving polyvinyl alcohol in purified water to obtain a polyvinyl alcohol solution, and immersing the printed bracket into the polyvinyl alcohol solution for crosslinking;
sintering the artificial bone scaffold with the crosslinked structure to obtain the hectorite 3D printing artificial bone scaffold;
the mass ratio of the hectorite to the purified water is 1:3-5;
the mass fraction of the polyvinyl alcohol solution is 4% -10%.
2. The method for preparing the hectorite 3D printing artificial bone scaffold according to claim 1, wherein a host bone prototype model is obtained after data is processed by using a software 3D Slicer, an applicable scaffold three-dimensional model is designed by using Free CAD software, the model is stored as an STL file and loaded into PC Printer software, and a bioceramic Printer is used for carrying out silk-Free 3D printing.
3. The method for preparing the hectorite 3D printing artificial bone scaffold according to claim 1, wherein in the crosslinking process, the crosslinking time is 3-7 h.
4. The method for preparing the hectorite 3D printing artificial bone scaffold according to claim 1, wherein in the sintering process, the sintering temperature is 820-870 ℃, and the heat preservation time is 3-5 h.
5. A hectorite 3D printed artificial bone scaffold made by the method of any one of claims 1-4.
6. The laponite 3D printed artificial bone scaffold of claim 5, wherein the pore structure is 30 ° -90 ° filled and the porosity is 40% -80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210736957.7A CN115054725B (en) | 2022-06-27 | 2022-06-27 | Hectorite 3D printing artificial bone scaffold and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210736957.7A CN115054725B (en) | 2022-06-27 | 2022-06-27 | Hectorite 3D printing artificial bone scaffold and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115054725A CN115054725A (en) | 2022-09-16 |
| CN115054725B true CN115054725B (en) | 2023-09-15 |
Family
ID=83202412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210736957.7A Active CN115054725B (en) | 2022-06-27 | 2022-06-27 | Hectorite 3D printing artificial bone scaffold and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115054725B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107235721A (en) * | 2017-05-02 | 2017-10-10 | 烟台正海生物科技股份有限公司 | A kind of porous Bredigites biological ceramic support of 3 D-printing and preparation method and application |
| WO2018090190A1 (en) * | 2016-11-15 | 2018-05-24 | 深圳先进技术研究院 | Clay-based hydrogel matrix for three-dimensional printing, preparation method therefor and use thereof |
| CN109200339A (en) * | 2018-09-12 | 2019-01-15 | 华东理工大学 | A kind of composite material, feedstock composition, bone repair, preparation method and application |
| CN111070376A (en) * | 2019-12-25 | 2020-04-28 | 西安点云生物科技有限公司 | 3D printing bionic porous bioceramic artificial bone and preparation method thereof |
| CN112960988A (en) * | 2021-02-02 | 2021-06-15 | 烟台正海生物科技股份有限公司 | 3D printing cuttable biological ceramic support and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020132875A1 (en) * | 2000-12-29 | 2002-09-19 | Dental Technologies, Inc. | Solid nanocomposites and their use in dental applications |
-
2022
- 2022-06-27 CN CN202210736957.7A patent/CN115054725B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018090190A1 (en) * | 2016-11-15 | 2018-05-24 | 深圳先进技术研究院 | Clay-based hydrogel matrix for three-dimensional printing, preparation method therefor and use thereof |
| CN107235721A (en) * | 2017-05-02 | 2017-10-10 | 烟台正海生物科技股份有限公司 | A kind of porous Bredigites biological ceramic support of 3 D-printing and preparation method and application |
| CN109200339A (en) * | 2018-09-12 | 2019-01-15 | 华东理工大学 | A kind of composite material, feedstock composition, bone repair, preparation method and application |
| CN111070376A (en) * | 2019-12-25 | 2020-04-28 | 西安点云生物科技有限公司 | 3D printing bionic porous bioceramic artificial bone and preparation method thereof |
| CN112960988A (en) * | 2021-02-02 | 2021-06-15 | 烟台正海生物科技股份有限公司 | 3D printing cuttable biological ceramic support and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| Zehao Wang，et al..A Novel 3D-bioprinted Porous Nano Attapulgite Scaffolds with Good Performance for Bone Regeneration.International Journal of Nanomedicine.2020,第15卷第6947页左栏第3段、右栏第1段. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115054725A (en) | 2022-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110101904B (en) | Degradable regenerative medical material for promoting tissue in-situ regeneration and preparation method thereof | |
| CN105330285B (en) | A kind of 3D printing ZrO2Toughening bioactive ceramics powder body material and its preparation and application | |
| CN113018509B (en) | Composite osteochondral scaffold and preparation method and application thereof | |
| WO2019211449A1 (en) | Synthetic bone graft | |
| CN115177787B (en) | 3D printing composite bone repair material and preparation method and application thereof | |
| CN109133971A (en) | A kind of calcium phosphate/bioactivity glass bone repairing support and preparation method thereof | |
| CN108686265A (en) | A kind of magnetic resonance development hydrogel of 3D printing and its preparation method and application | |
| KR101481988B1 (en) | Method for preparing porous ceramic scaffold | |
| CN115607742A (en) | Composite hydrogel scaffold for promoting bone-cartilage repair and preparation method and application thereof | |
| CN115054725B (en) | Hectorite 3D printing artificial bone scaffold and preparation method thereof | |
| CN107050513A (en) | A kind of gradient dip-coating HA prepares ZrO2The method of Bone Defect Repari bioceramic scaffold material | |
| Kakarla et al. | Boron nitride nanotubes reinforced gelatin hydrogel-based ink for bioprinting and tissue engineering applications | |
| CN103191470A (en) | Organic/inorganic composite three-dimensional porous scaffold with drug sustained release function, and preparation method thereof | |
| SE537634C2 (en) | Titandioxidscaffold | |
| CN108638494B (en) | Preparation method of calcium phosphate porous scaffold | |
| CN112704767B (en) | Personalized customized bone organ manufactured accurately in 4D mode and preparation method thereof | |
| CN115137876A (en) | Hectorite bioceramic artificial bone and preparation method thereof | |
| CN114855024A (en) | Porous tantalum medical implant material and preparation method and application thereof | |
| CN107041968A (en) | One kind prepares ZrO2The HA dip-coating slurries of Bone Defect Repari bioceramic scaffold material | |
| CN106178099A (en) | The forming technique of direct write without mould prepares the method for titanium/hydroxylapatite biology support | |
| CN114288477A (en) | Recombinant collagen hydrogel 3D printing ink and application thereof | |
| CN111701074A (en) | Polydopamine hybrid agarose hydrogel, and preparation method and application thereof | |
| CN111921011A (en) | Artificial bone coated with coating and preparation method thereof | |
| CN109330743A (en) | A kind of 3D printing tissue engineering bracket and preparation method thereof | |
| CN116161955B (en) | Normal-temperature one-step extrusion 3D printing forming preparation method of high-strength hydroxyapatite bone tissue engineering scaffold |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |