CN115136887B - Method for cultivating triploid purple rain birch - Google Patents
Method for cultivating triploid purple rain birch Download PDFInfo
- Publication number
- CN115136887B CN115136887B CN202210931061.4A CN202210931061A CN115136887B CN 115136887 B CN115136887 B CN 115136887B CN 202210931061 A CN202210931061 A CN 202210931061A CN 115136887 B CN115136887 B CN 115136887B
- Authority
- CN
- China
- Prior art keywords
- birch
- purple
- triploid
- rain
- white birch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
Abstract
The invention discloses a method for cultivating triploid purple rained birch, and belongs to the field of forest breeding. The method comprises the following steps: (1) White birch seed is induced by colchicine to be cultivated into tetraploid plant; (2) Hybridizing the purple-rain birch and the northeast white birch which are used as parents to obtain a diploid purple-rain birch plant; (3) Taking diploid purple birch as a male parent, collecting pollen, drying in the shade for 24-48 hours, and preserving in vacuum for later use; (4) Taking tetraploid white birch as female parent, removing male inflorescence, and pollinating at 9-10 am; (5) And (5) continuously cultivating after pollination to obtain triploid purple rain birch seedlings. According to the invention, the triploid purple rain birch is obtained by combining cross breeding and ploidy breeding through leaf color investigation, and the new variety has more excellent cold resistance and has important significance for improving the ornamental value of the purple rain birch and beautifying the urban environment.
Description
Technical Field
The invention relates to the field of forest breeding, in particular to a method for cultivating triploid purple rain birch.
Background
The purple birch (Betula pendula 'PurpleRain') is a color-leaf tree variety from Betula alba (Betula pendula Roth), has purple leaves, grey-white trunk and high ornamental value, and is widely applied to courtyard decoration and urban greening in Europe and North America. The diploid betula alreuteri is introduced in 2001 in Halbin, and after years of experiments, the fact that the growth of the variety is late after autumn, the tops of branches are usually damaged by early frost, the trunk and the side branches are bent, and the ornamental value is obviously reduced. Therefore, the improvement of the cold resistance of the purple rain birch is a problem which is urgently needed to be solved at present.
The polyploid of the forest tree generally has the characteristics of vigorous growth, large leaf, strong stress resistance and the like, is an effective way for genetic improvement of the forest tree, and is also successful experience verified by Chinese and foreign scholars through practice. For example, johnsson et al have shown that the hybridization of betula heterotetraploids (B.japonica. Times.B.verrucosa) with betula diploids produces heterotrimeric progeny that are significantly superior in growth to betula copula homologous progeny, white birch triploid breeding proves that the bred white birch tetraploid is used as a female parent to be hybridized with a white birch diploid, and a fast-growing and anti-adversity white birch family and an excellent single plant are bred. In particular, poplar has achieved some benefit in triploid breeding. The natural triploid of the European aspen and the American aspen and the hybrid triploid thereof are popularized and widely applied in forestry production nowadays, and the application of the Chinese aspen triploid brings huge economic benefits to Chinese farmers. At present, no relevant report on breeding of the triploid purple birch exists.
The creation and breeding of the purple rain birch triploid is one of the ways of improving the cold resistance of the purple rain birch triploid. The breeding method has the advantages that the crossbreeding work of taking the excellent white birch tetraploid as the female parent and the excellent purple rain birch diploid is carried out, and the cold-resistant new species is bred, so that the breeding method has important significance for improving the cold resistance and the ornamental value of the purple rain birch and beautifying the urban environment.
Disclosure of Invention
The invention aims to provide a method for cultivating triploid purple rained birch, which solves the problems in the prior art. The excellent white birch tetraploid is used as a female parent to perform hybrid breeding with the excellent purple rain birch diploid, so that a new white birch variety with the excellent purple rain can be obtained, and the new white birch variety has more excellent cold resistance and has important significance for improving the appreciation of the purple rain birch and beautifying the urban environment.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for cultivating triploid purple rained birch, which is characterized by comprising the following steps:
(1) Soaking seeds of white birch, inducing with colchicine, and culturing into tetraploid white birch plant;
(2) Carrying out hybrid breeding by taking the purple rain birch and the northeast white birch as parents to obtain hybrid offspring, selecting axillary buds on hybrid offspring branches, and carrying out tissue culture to obtain diploid purple rain birch plants;
(3) When the diploid purple rained birch in the step (2) begins to loose pollen, picking up male inflorescences, collecting pollen, drying in the shade for 24-48 hours, and preserving in vacuum for later use;
(4) Selecting branches with female flowers in the tetraploid white birch in the step (1), removing male inflorescences, and bagging for pollination; the pollination time is 9-10 am; the pollination is pollen preserved in vacuum in the step (3);
(5) And (5) continuously cultivating after pollination to obtain the triploid purple rained birch seedling.
Further, in the step (1), the seed soaking time is 1d; the colchicine induction is specifically as follows: soaking seeds for 2d at 30 ℃ according to the proportion of adding 30mL of colchicine solution into 300 seeds; the mass concentration of the colchicine is 0.2 percent.
Further, in the step (1), after colchicine induction, seed germination is also included, and after cotyledons grow out, the seeds are transplanted into a sterilized matrix for cultivation.
Further, in the step (2), the tissue culture comprises primary culture and secondary culture of adventitious buds, wherein the culture medium components of the primary culture comprise WPM culture medium +1.0 mg/L6-BA; the components of the culture medium for subculture comprise WPM culture medium +1.0 mg/L6-BA +0.02mg/LNAA.
Further, in the step (2), the purple rained birch is a 5-year-old plant; the northeast white birch is a 7-year-old plant.
Further, in the step (2), the axillary buds further comprise a disinfection treatment before tissue culture.
Further, the sterilization process includes: picking axillary buds, placing in 70% ethanol solution for 1min, rinsing with sterile water for 1min, removing outer bud scale, and adding HgCl with volume fraction of 0.1% 2 Soaking for 8min until the quality is goodCa (ClO) in an amount of 10% 2 Soaking in the solution for 10min, and rinsing with sterile water for 3-5 times.
The invention discloses the following technical effects:
according to the invention, a means of combining cross breeding and ploidy breeding is adopted, and the triploid purple rainblow is obtained through leaf color investigation, and has important significance for improving the cold resistance and the ornamental value of the purple rainblow and beautifying the urban environment due to the characteristics of cold resistance, red and green saturation and high anthocyanin content of the triploid purple rainblow.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 shows the result of chromosome ploidy analyzer detection of hybrid progeny No. Q33 × purple nepheline No. 1 and Q33 × purple nepheline No. 2, wherein a is the result of detection of purple rainbirch in hybrid progeny, and b is the result of detection of white birch in hybrid progeny;
FIG. 2 shows the leaf color separation of the progeny of the hybrids Q33X cancrina No. 1 and Q33X cancrina No. 2;
FIG. 3 shows phenotype of triploid purple birch (left) and triploid white birch (right) plants in hybrid progeny Q33 × purple nepheline No. 1 and Q33 × purple nepheline No. 2;
FIG. 4 shows leaf phenotype of triploid purple birch (left) and triploid white birch (right) in hybrid progeny Q33X purple nepheline No. 1 and Q33X purple nepheline No. 2;
FIG. 5 shows the condition of branching caused by freezing damage of the terminal buds of triploid purple birch and triploid white birch in filial generation of Q33 × purple nepheline No. 1 and Q33 × purple nepheline No. 2;
FIG. 6 shows the area growth of triploid purple birch and triploid white birch and common diploid white birch in hybrid progeny Q33X purple nephelin No. 1 and Q33X purple nephelin No. 2.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in the present disclosure, it is understood that each intervening value, to the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The specification and examples are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
Mature seeds of the white birch, the 5-year-old purple birch and the 7-year-old northeast white birch used in the embodiment are all derived from white birch elite base of northeast forestry university.
Example 1
1. Cultivation of white birch tetraploid mother tree Q33
(1) The mature embryo of the white birch is treated by colchicine to induce tetraploid:
soaking mature seeds of the white birch in distilled water for 1 day, adding 30mL of colchicine with the mass concentration of 0.2% into 300 seeds, continuously soaking the seeds for 2 days at the temperature of 30 ℃, taking out the seeds, placing the seeds in a culture dish filled with moist filter paper for germination, and transplanting the seeds into a seedling culture tray filled with a sterilized matrix for culture after cotyledons grow out after 7 days.
(2) Ploidy identification of tetraploid seedlings
After 3-4 leaves grow out from the nursery stock, taking the leaves respectively, bringing the leaves back to a laboratory by a crushed ice fresh-keeping method, and carrying out ploidy determination by adopting a ploidy analyzer to obtain 200 white birch tetraploids successively.
(3) White birch tetraploid selection and planting
The height, the plant type, the leaf size and the like of the obtained tetraploid white birch in the seedling developing period are determined, field offspring is determined and the like, and a tetraploid white birch single plant (Q33) with excellent comprehensive properties is obtained through screening.
2. Cultivation of diploid purple rain birch mother tree ' purple nephelin No. 1 ' and ' purple nephelin No. 2
The method comprises the steps of taking a 5-year-old purple birch (Betula pendula 'PurpleRain') of a national fine breed base of the white birch of the university of northeast forestry as a female parent and a male parent as a 7-year-old northeast white birch (Betula platyphylla), collecting the pollen of the northeast white birch in 20/2011, selecting branches with female inflorescences from the purple birch, removing bags after the male inflorescences on the upper parts of the branches, pollinating when mucus begins to be secreted by stigma, performing operations of seed collection, airing, seed cleaning and the like after the fruit cluster grows mature, and seeding and seedling in the early 5/2012 month. Obtaining more than 500 purple rain birch strains, respectively investigating seedling height, leaf color separation condition and freezing damage condition of hybrid offspring in 2 years, preliminarily selecting 4 excellent purple rain birch single strains (Z1, Z2, Z3 and Z4), respectively collecting branches with axillary buds of the 4 excellent single strains in 10 th of 2013, washing for 2 days with running water, picking up the axillary buds, placing in 70% ethanol for 1min, rinsing for 1min with sterile water, stripping outer layer bud scale on a clean bench, and 0.1% (W/V) HgCl 2 Soaking in solution for 8min, with 10% calcium hypochlorite (ClO) 2 Soaking in the solution for 10min, noneRinsing with bacteria water for 3-5 times. Inoculating the sterilized axillary buds into WPM +1.0 mg/L6-Benzylaminopurine (6-Benzylaminopurine, 6-BA) for primary culture, transferring to WPM +1.0 mg/L6-BA +0.02mg/L NAA for subculture of adventitious buds after 30d, carrying out rooting culture on tissue culture seedlings in 3 months in 2014, transplanting the tissue culture seedlings into a seedling tray in middle ten days of 4 months, respectively carrying out propagation on 100 excellent single plants of Z1, Z2, Z3 and Z4, placing the seedlings in a greenhouse for conventional management, transplanting the seedlings into a flowerpot with 21cm multiplied by 21cm in early spring in 2015, carrying out cultivation in 2 years, carrying out investigation and analysis on the high seedling, leaf color and freezing damage condition of 400 strains of Z1, Z2, Z3 and Z4, wherein the leaf color of Z1 and Z2 is a formal spring brown in summer, the top color of the new variety is a dark green plant with a purple color of 2019, and the top color of the purple is a new variety with a color of 2019, namely, and the purple early season in 9012 Yuanxian-year is obtained; the leaves of Z2 are purple in spring, the upper surface of the new top shoot leaves are grayish reddish brown in summer, and a new plant variety right is also obtained in the same year and is formally named as 'purple Charactitis 2' (authorization number: 20190380).
3. Construction of triploid seed production garden
The excellent white birch tetraploid Q33 and the diploids 'purple Xia No. 1' and 'purple Xia No. 2' which are bred are used as mother trees and are planted in a shed type seed garden of a white birch national fine variety base of northeast forestry university, and the row spacing of the mother trees is 4m multiplied by 5m. And carrying out conventional measures such as water and fertilizer management, pest control and the like on the mother trees in the garden.
4. Creation of triploid purple rain birch
(1) Collection of pollen of male parent
Test groups: and 4, 20 days after 4 months, when the inflorescences of the canula purpurea 1 and the canula purpurea 2 begin to loose powder, picking the inflorescences, spreading the inflorescences on parchment paper, placing the parchment paper in a drying place to wait for powder scattering, continuously drying the parchment paper in the shade for 24 to 48 hours after the powder scattering, and then vacuumizing the parchment paper to store the parchment paper for later use at 4 ℃.
Control group 1: the difference with the test group is that after the powder is scattered, the drying is continued for 12 hours in the shade, and then the parchment paper is well wrapped and stored for standby at 4 ℃ after being vacuumized.
Control group 2: the method is different from the test group in that the pollen of the male parent is directly wrapped by parchment paper for storage for later use without being dried in the shade.
(2) Hybrid pollination
After 30 days in 4 months, a branch with a female inflorescence is selected by taking tetraploid white birch (Q33) in a garden as a female parent, a male inflorescence at the upper part of the branch is removed, a bag is sleeved, after mucus secretion of stigma begins, pollination is carried out by using pollen of 'purple light 1' and 'purple light 2' collected by a test group, a control group 1 and a control group 2 respectively, the female parent is randomly set to be 12 groups, and pollination information of each group with the number of 1-12 is shown in a table 1.
TABLE 1 pollination information for groups
In late 7 months, the growth and development of the infructescence are observed, the growth of the hybridized infructescence of the 4 th group and the 10 th group is best, most of the rest groups have no infructescence, which indicates that the hybridization fails, and in addition, a small part of the group has infructescence but poor infructescence or falls off when the group is not mature. The reasons for the failure of other hybridization combinations are as follows: (1) pollen viability is reduced or lost. That is, the pollen collected by the control group has short drying time in the shade, or is not dried in the shade and directly wrapped by parchment paper for storage, the pollen which is not dried gradually loses activity in the storage process, and the pollination by the pollen can cause the fruit sequence to develop badly or fall off; (2) pollination times are not appropriate. The pollination time of the control group is 6-7 in the early morning, the temperature is low at the moment, the pollen tube is not beneficial to growth, the pollen only plays a role in stimulating stigma, and although the fruit is generated, the pollen falls off spontaneously due to dysplasia in the later period.
Thus, groups 4 and 10 were selected for subsequent studies.
(3) Seed collection, seedling raising and ploidy identification:
in late 7 months, after the fruit sequence is mature, the operations of seed collection, airing and seed cleaning are carried out, the seedlings are sowed in seedling-raising trays (25 holes) of 40cm multiplied by 40cm at the beginning of 8 months, and the seedlings are waiting for the seedlings to grow4-5 leaves, respectively selecting Q33X Chardonia 1 hybrid progeny 104 strains and Q33X Chardonia 2 hybrid 157 seedling, picking young leaves, removing midrib, and placing about 0.5cm X0.5 cm leaves in a glass culture dish; 2mL of pre-cooled extraction buffer (10 mmol/L MgSO. Sub.m.) was added 4 ·7H 2 O solution, 50mmol/L KCl solution, 5mmol/L HEPES solution, 1% (W/V) PVP-40,0.25% (V/V) Triton X-100, pH = 8.0), mesophyll was quickly minced vertically with a unimodal blade, 1ml DAPI staining solution (Partec) was added; the sample was filtered through a 30 μm cell sieve into the sample tubes and ploidy was determined using a chromosome ploidy analyzer (PA, partec, germany), and the progeny of the cross were triploid (FIG. 1).
5. Leaf color separation and cold resistance comparison of triploid offspring
Leaf color separation and freezing damage of hybrid offspring are investigated in the current seedling stage of seedling culture, and leaf color investigation of hybrid combination offspring seedlings of No. Q33X purple nepheline 1 finds that 53 plants are purple-leaf purple-rain birch (ZY 1) and 51 plants are green-leaf white birch (LY 1) in 104 triploid offspring; in the Q33 × purple nepheline No. 2 triploid progeny, 73 plants were purple-leaf purple rainbirch (ZY 2) and 84 plants were green-leaf white birch (LY 2), and accordingly, the purple leaves were considered: the proportion of green leaves was close to 1 (FIGS. 2-4).
Because the nursery stocks are grown in 8 months, the terminal buds of the nursery stocks do not form full dormant buds after 10 months of growth, the early frost in the Harbin region can cause freezing damage to the seedlings, in order to compare the cold resistance of the triploid purple birch and the triploid white birch, the nursery stocks are tested to overwinter under the condition of not performing cold-proof, whether the terminal buds grow to straight stems or branch due to the freezing damage of the terminal buds is investigated in the next 5 months, the cold resistance of the triploid family is further evaluated, and the investigation result shows that: the percentage of the branch-producing lines in the triploid purple rain birch ZY1 and ZY2 to the total number of the strains ZY1 and ZY2 is 3.92 percent and 8.00 percent respectively; the percentage of triploid birch LY1 and LY2 producing branched lines to the total number of LY1 and LY2 was 8.16% and 25.64%, respectively. It was shown that the cold tolerance of triploid purple rained birch was significantly higher than that of triploid white birch (fig. 5).
6. Leaf color and growth characteristics of triploid purple rained birch
Transplanting overwintering seedlings into flowerpots with the diameter of 21cm respectively at the beginning of 5 months, and placing the flowerpots in a plastic shed for culturing. Measuring leaf brightness, anthocyanin content, chlorophyll content, leaf length, leaf width and plant height by using a spectrocolorimeter (CR-400, japan), a portable anthocyanin content measuring instrument (OPTI-SCIENCES ACM-200 plus) and a portable chlorophyll measuring instrument (SPAD-502 PLUS, KONICA MINOLTA) respectively at 15 days in 5 months, 1 days in 6 months, 15 days in 6 months and 1 day in 7 months; measuring an L value and an a value by using a CR-400 color difference meter, wherein the L value represents the brightness degree of the blade, and the higher the value is, the higher the brightness of the blade is; a indicates red-green saturation, + indicates red bias, and-indicates green bias. The results of measurement are shown in Table 2-6 (ZY 1 and ZY2 represent triploid purple rained birch, LY1 and LY2 represent triploid white birch, BH represents diploid white birch).
TABLE 2 comparison of leaf color values L of plants at different developmental stages
Note: those in the same column followed by the same letter indicate no significant difference at the P < 0.05 level.
TABLE 3 comparison of leaf color a of plants at different developmental stages
Note: those with the same letters after the same column indicate no significant difference at P < 0.05.
TABLE 4 comparison of the relative content of anthocyanin in plants at different developmental stages
Note: those in the same column followed by the same letter indicate no significant difference at the P < 0.05 level.
TABLE 5 comparison of relative chlorophyll content in plants at different developmental stages
Note: those with the same letters after the same column indicate no significant difference at P < 0.05.
TABLE 6 plant height comparison of plants at different developmental stages
Note: those in the same column followed by the same letter indicate no significant difference at the P < 0.05 level.
The results show that the L values of the triploid purple rained birch (ZY) were significantly lower than those of the triploid white birch (LY) and the diploid white Birch (BH) in the 4 stages investigated in terms of the degree of leaf darkening (table 2); in terms of red-green saturation, triploid purple rained birch is significantly higher than triploid white birch and diploid white birch (table 3); in terms of the relative content of anthocyanin, the anthocyanin content of the triploid purple rained birch is remarkably higher than that of the triploid white birch and the diploid white birch in 4 periods (table 4); in terms of relative chlorophyll content, the early stage of leaf development is that the chlorophyll content of the triploid purple rained birch is significantly higher than that of the white birch, and no significant difference exists by 7 months and 1 day (table 5); in terms of plant height growth, triploid purple rainy birch was significantly lower than diploid and triploid white birch (table 6); in terms of leaf area, the triploid purple rained birch is significantly higher than that of diploid white birch, the average value of the leaf area of the purple rained birch of 2 families is 65.94% higher than that of the diploid white birch, and the growth amount of the purple rained birch leaves is obviously reduced compared with that of the triploid white birch (figure 6).
In conclusion, the triploid purple rain birch has excellent cold resistance, and meanwhile, the leaves have good red-green saturation, bright color and higher ornamental value.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (9)
1. A cultivation method of triploid purple rain birch is characterized by comprising the following steps:
(1) Soaking seeds of white birch, inducing with colchicine, and culturing into tetraploid white birch plant;
(2) Carrying out hybrid breeding by taking the purple rain birch and the northeast white birch as parents to obtain hybrid offspring, selecting axillary buds on hybrid offspring branches, and carrying out tissue culture to obtain diploid purple rain birch plants;
(3) When the diploid purple rained birch in the step (2) begins to loose pollen, picking up male inflorescences, collecting pollen, drying in the shade for 24-48 hours, and preserving in vacuum for later use;
(4) Selecting branches with female flowers in the tetraploid white birch in the step (1), removing male inflorescences, and bagging for pollination; the pollination time is 9-10 am; the pollination is pollen preserved in vacuum in the step (3);
(5) And (5) continuously cultivating after pollination to obtain triploid purple rain birch seedlings.
2. The cultivation method as claimed in claim 1, wherein, in the step (1), the seed soaking time is 1d; the colchicine induction is specifically as follows: soaking seeds for 2 days at 30 ℃ according to the proportion that 30mL of colchicine solution is added into 300 seeds, wherein the mass concentration of the colchicine solution is 0.2%.
3. The cultivation method as claimed in claim 1, wherein in step (1), after colchicine induction, seeds are germinated and transplanted into a sterilized substrate for cultivation after cotyledons grow out.
4. The cultivation method as claimed in claim 1, wherein, in step (2), the tissue culture includes primary culture and subculture of adventitious buds.
5. The culture method of claim 4, wherein the medium component of the primary culture comprises WPM medium +1.0 mg/L6-BA.
6. The culture method according to claim 4, wherein the medium components of the subculture comprise WPM medium +1.0 mg/L6-BA +0.02mg/LNAA.
7. The cultivation method according to claim 1, wherein in step (2), the purple rained birch is a 5-year-old plant; the northeast white birch is a 7-year-old plant.
8. The cultivation method as claimed in claim 1, wherein in the step (2), the axillary buds further comprise a sterilization treatment before the tissue culture.
9. Incubation method according to claim 8, characterized in that said sterilisation treatment comprises: picking axillary buds, placing in 70% ethanol solution for 1min, rinsing with sterile water for 1min, removing outer layer bud scale, and adding HgCl with volume fraction of 0.1% 2 Soaking for 8min in water, and adding 10% of Ca (ClO) 2 Soaking in the solution for 10min, and rinsing with sterile water for 3-5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210931061.4A CN115136887B (en) | 2022-08-04 | 2022-08-04 | Method for cultivating triploid purple rain birch |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210931061.4A CN115136887B (en) | 2022-08-04 | 2022-08-04 | Method for cultivating triploid purple rain birch |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115136887A CN115136887A (en) | 2022-10-04 |
| CN115136887B true CN115136887B (en) | 2023-01-10 |
Family
ID=83413895
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210931061.4A Active CN115136887B (en) | 2022-08-04 | 2022-08-04 | Method for cultivating triploid purple rain birch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115136887B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115715522A (en) * | 2022-12-06 | 2023-02-28 | 东北林业大学 | Method for creating purple birch with cracked leaves |
| CN115997683B (en) * | 2023-02-09 | 2023-10-20 | 东北林业大学 | Efficient creating method for tetraploid betula platyphylla |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502237A (en) * | 2009-01-24 | 2009-08-12 | 东北林业大学 | Micro-propagation method for white birch with purple leaf |
| CN103125399A (en) * | 2013-03-20 | 2013-06-05 | 东北林业大学 | Rapid issue culture propagation method for white birch tetraploid |
| CN107135942A (en) * | 2017-06-04 | 2017-09-08 | 滨州市沾化区冬枣实业总公司 | A kind of breeding method of triploid winter jujube |
-
2022
- 2022-08-04 CN CN202210931061.4A patent/CN115136887B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101502237A (en) * | 2009-01-24 | 2009-08-12 | 东北林业大学 | Micro-propagation method for white birch with purple leaf |
| CN103125399A (en) * | 2013-03-20 | 2013-06-05 | 东北林业大学 | Rapid issue culture propagation method for white birch tetraploid |
| CN107135942A (en) * | 2017-06-04 | 2017-09-08 | 滨州市沾化区冬枣实业总公司 | A kind of breeding method of triploid winter jujube |
Non-Patent Citations (6)
| Title |
|---|
| Morphological Variation among Betula nana (diploid), B. pubescens (tetraploid) and their Triploid Hybrids in Iceland;Æ. Th. Thórsson等;《Annals of Botany》;20070411;第99卷(第6期);1183-1193 * |
| Natural hybridisation in birch: triploid hybrids between Betula nana and B. pubescens;Kesara Anamthawat-Jónsson & Aegir Thór Thórsson;《Plant Cell, Tissue and Organ Culture》;20031231;第75卷;99-107 * |
| Research progress on genetic improvement of Betula platyphylla Suk.;Chuanping YANG;《Front. Agr. Sci. Eng.》;20171231;第4卷(第4期);391-401 * |
| 彩叶桦‘紫霞1号’新品种;刘桂丰等;《南京林业大学学报( 自然科学版)》;20210930;第45卷(第5期);245-246 * |
| 白桦不同杂交组合三倍体子代当年生苗木生长性状分析;林琳等;《北京林业大学学报》;20120930;第34卷(第5期);1-5 * |
| 紫雨桦花色素苷含量的时空变化及相关基因的表达特性分析;姜晶等;《西南林业大学学报(自然科学)》;20170315;第37卷(第02期);53-59 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115136887A (en) | 2022-10-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115136887B (en) | Method for cultivating triploid purple rain birch | |
| CN113287513A (en) | Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity | |
| CN107278891B (en) | A kind of apricot plum quick breeding method for tissue culture | |
| CN104145818A (en) | Preservation method of gerbera germplasm resources | |
| CN113057099B (en) | Simplified tissue culture method of tilia europaea | |
| Sabir et al. | Qualitative and quantitative responses of early ripening table grape cultivars (Vitis vinifera L.) to pollination treatments under controlled growing condition | |
| JP7248367B2 (en) | Method for production of seed with improved seed germination properties | |
| Abul-Soad et al. | Date palm somatic embryogenesis from inflorescence explant | |
| US5304725A (en) | Elite white spruce hybrids and method of production | |
| CN111280054B (en) | Tissue culture proliferation and rooting integrated seedling formation method for hydrangea convata and culture medium used by tissue culture proliferation and rooting integrated seedling formation method | |
| Mohamed et al. | Enhanced haploids regeneration in anther culture of summer squash (Curcurbita pepo L.) | |
| Kwon et al. | Tetraploid induction approach induced by colchicine of Prunella vulgaris for. albiflora Nakai | |
| CN113785772A (en) | Method for inducing cucurbit haploid embryo | |
| Jitsopakul et al. | Interspecific and intergeneric hybrids of Aerides species with Rhynchostylis coelestis Rchb. f. and germination of hybrid seeds in vitro | |
| CN110959530A (en) | Non-distance calanthe field regression method | |
| Ajdarbin et al. | Indirect Shoot Regeneration in Anthurium andreanum ‘Clisto’from Leaf Explant | |
| Rahman et al. | Cytomorphological characterization of tea cultivars | |
| CN115443902B (en) | Breeding method for overcoming obstacle before fertilization of distant hybridization of muscadine grapes and true grapes and identification method thereof | |
| CN102440177B (en) | Method for cultivating new cactus variety redtop | |
| US11013191B2 (en) | Method for growing somatic embryos of conifers into trees | |
| CN109156345B (en) | Micropropagation method for tissue at top end of reproductive bud of six flowers | |
| KR20180133732A (en) | New Aralia elata variety | |
| CN100387113C (en) | Tissue cultivation quick breeding method for red leaf Chinese toon | |
| Sun et al. | The Technical System of Rapid Propagation with Photinia frasery Leaves Inducing Calli, First Generation Culture, Increasing Reproduction, Rooting and Training Seedlings to Transplant | |
| CN115462303A (en) | Seedling raising method for gramineous plants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |