CN115124186A - Method for treating aged micro-plastic wastewater by using microalgae - Google Patents
Method for treating aged micro-plastic wastewater by using microalgae Download PDFInfo
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- CN115124186A CN115124186A CN202210550023.4A CN202210550023A CN115124186A CN 115124186 A CN115124186 A CN 115124186A CN 202210550023 A CN202210550023 A CN 202210550023A CN 115124186 A CN115124186 A CN 115124186A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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Abstract
The invention belongs to the field of wastewater treatment, and particularly relates to a method for treating aged micro-plastic wastewater by using microalgae, which comprises the following steps: 1) pre-culturing functional microalgae to reach logarithmic growth phase; 2) respectively aging the micro plastic wastewater by using hydrochloric acid or sodium hydroxide; 3) and inoculating the functional microalgae cultured in the step 1) into the micro plastic wastewater respectively pretreated by hydrochloric acid or sodium hydroxide in the step 2), and purifying the high-concentration aged micro plastic wastewater. The application provides a green, environment-friendly, economical, effective and sustainable method for purifying high-concentration aged micro-plastic wastewater.
Description
Technical Field
The invention belongs to the field of wastewater treatment, and particularly relates to a method for treating aged micro-plastic wastewater by using microalgae.
Background
The biopolymer purification of aged microplastics in microalgae is a sunlight-driven, low-cost and environmentally friendly technology. In recent years, the technology of purifying aged microplastics using microalgae has gained increasing attention due to the promotion of sewage discharge standards and the demand for environmentally friendly technologies in carbon and background. The microalgae can generate Extracellular Polymeric Substance (EPS) due to adaptive reaction in the environment of high-concentration aged microplastic, and the hydrophobic property of the aged microplastic promotes the aggregation between the microalgae and the high-concentration aged microplastic, so that the potential of the microalgae for purifying the high-concentration aged microplastic wastewater is reflected.
Although there are many advantages to the technology of contaminant purification based on biopolymers of microalgae, there are a wide variety of contaminants in nature, which cannot guarantee non-selective purification of contaminants, and it is therefore necessary to seek to enhance the purification capacity of microalgae remediation technologies in combination with other green means.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for treating aged micro-plastic wastewater by using microalgae.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for treating aged micro-plastic wastewater by using microalgae comprises the following steps:
1) pre-culturing the functional microalgae to reach the logarithmic growth phase;
2) respectively aging the micro plastic wastewater by using strong acid or strong base;
3) and inoculating the functional microalgae cultured in the step 1) into the micro plastic wastewater respectively pretreated by strong acid or strong base in the step 2) to purify the high-concentration aged micro plastic wastewater.
The functional microalgae in the step 1) is Scenedesmus quadricauda or chlorella vulgaris.
The pre-culture conditions in the step 1) are that the whole day illumination, the illumination intensity of 4800- 2 And N 2 Mixed gas of (2), CO 2 The volume fraction is 3% -5%, and the ventilation volume is 30-40 mL/min.
The strong acid in the step 2) is hydrochloric acid, sulfuric acid or nitric acid; the strong base is sodium hydroxide or potassium hydroxide.
The concentration of strong acid or strong base is 5-20mol/L, and the treatment time is 24-48 h.
The concentration of strong acid or strong base is 10mol/L, and the treatment time is 48 h.
In the step 2), the micro plastic is polyethylene glycol terephthalate, the size is 5-50 mu m, and the concentration of the micro plastic after being treated by strong acid or strong alkali is 20-200 mg/L.
Compared with the prior art, the invention has the beneficial effects that:
the application provides a green, environment-friendly, economical, effective and sustainable method for purifying high-concentration aged micro-plastic wastewater. And (3) purifying the wastewater containing the high-concentration aged micro-plastics by using microalgae, purifying pollutants by using photosynthesis of the microalgae, and obtaining microalgae biomass and cytochrome. The microalgae purification mode can enable the microalgae to generate more negatively charged Extracellular Polymeric Substances (EPS), and has potential purification potential for aged microplastics. And simultaneously, the aged micro-plastic wastewater is pretreated by strong acid or strong base so as to reach the natural aging degree in a short time.
Drawings
FIG. 1 is a graph showing the intensity of uronic acid peak in EPS produced from Chlorella (after 8 days of culture) in the examples;
FIG. 2 shows the embodiment of adding strong alkali to age the chlorella OD in the micro plastic wastewater 680 And (5) a variation graph.
FIG. 3 shows the addition of strong acid to the chlorella OD in the wastewater 680 A variation graph;
FIG. 4 is a graph showing the change of chlorophyll concentration in the waste water of the aged micro plastic added with strong alkali in the example;
FIG. 5 is a graph showing the change of chlorophyll concentration in wastewater from the aging of micro-plastic with strong acid added in the example;
FIG. 6 is a graph showing the change in carotenoid concentration in the waste water of the aged micro plastic with the addition of strong alkali in the examples;
FIG. 7 is a graph showing the change in carotenoid concentration in wastewater from the addition of a strong acid-aged micro plastic in the examples.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings and preferred embodiments.
The implementation case is as follows: a method for treating aged micro-plastic wastewater by using microalgae comprises the following steps:
(1) inoculating Chlorella UTEX1602 into sterilized BG-11 medium 200mL, placing in biochemical incubator, and pre-culturing to logarithmic ChlorellaGrowing period, and taking it as working algae liquid; the conditions of the pre-culture were set as: illumination all day long, illumination intensity 4800- 2 And N 2 Mixed gas of (2), CO 2 The occupied volume fraction is 5%.
(2) Respectively treating the waste water containing the high-concentration aged micro-plastics by using hydrochloric acid or sodium hydroxide for 48 hours to artificially simulate the treatment, so as to obtain a waste water treatment solution; the concentration of the hydrochloric acid or the sodium hydroxide is 10 mol/L;
the preparation method of the artificial simulation wastewater containing the high-concentration aged micro-plastics comprises the steps of adding a certain amount of 4g/L aged micro-plastic mother liquor (the micro-plastics are polyethylene glycol terephthalate and have the size of 10 mu m; the micro-plastics are added into 200mL BG-11 culture medium) to obtain wastewater containing 20, 100 and 200mg/L aged micro-plastics;
(3) washing and centrifuging the algae solution obtained in the step (1) by deionized water for 3 times, inoculating the algae solution into the artificial simulated high-concentration aged micro-plastic-containing wastewater treated by strong acid or strong base in the step (2), culturing the wastewater on a microalgae culture rack to ensure that the absorbance of the initially inoculated microalgae is 0.1 at 680nm wavelength, illuminating the microalgae all day at 4800-5200Lux illumination intensity at 24-26 ℃ for 8 days in a culture period, and sampling to determine the content of uronic acid in the microalgae EPS.
(4) Periodically collecting the algae solution cultured in step (3), and measuring the absorbance of the algae solution at 680nm wavelength.
(5) And (4) periodically collecting the algae liquid cultured in the step (3), centrifuging and removing the supernatant to obtain algae mud, re-suspending the harvested algae mud in methanol-water solution with the same volume, storing at 4 ℃ for 16-24h, centrifuging and measuring the absorbance of the supernatant at multiple wavelengths, and measuring the intracellular concentration of the photochromic pigment.
The control group differs from the examples in that the medium does not require the addition of aged microplastics.
Namely, the method for treating the wastewater by the microalgae comprises the following steps:
(1) inoculating chlorella UTEX1602 to sterilized BG-11 medium containing 200mL, placing in biochemical incubator, and pre-culturing to make chlorella reach logarithmic phaseThen the algae solution is used as working algae solution; the conditions of the pre-culture were set as: illumination all day long, illumination intensity 4800- 2 And N 2 Mixed gas of (2), CO 2 The volume fraction is 5%.
(2) Washing and centrifuging the algae solution obtained in the step (1) by deionized water for 3 times, inoculating the algae solution into water, culturing the algae solution on a microalgae culture rack to ensure that the absorbance of the initially inoculated microalgae is 0.1 at the wavelength of 680nm, illuminating the microalgae all day with the illumination intensity of 4800-.
(3) Periodically collecting the algae solution cultured in step (2), and measuring the absorbance of the algae solution at 680nm wavelength.
(4) Collecting the algae liquid cultured in the step (2) periodically, centrifuging, removing the supernatant to obtain algae mud, re-suspending the harvested algae mud in methanol-water solution with the same volume, storing at 4 ℃ for 16-24h, centrifuging, measuring the absorbance of the supernatant at multiple wavelengths, and measuring the intracellular concentration of the photochromic pigment.
After the treatment of the aged microplastic by adding strong acid or strong base respectively as shown in fig. 1, after 8 days of culture, the content of uronic acid in EPS generated by the aged microplastic in the artificial simulation wastewater of 20, 100 and 200mg/L by the microalgae is respectively 8.36% lower and 45.48% higher than that of the control group.
FIG. 2 shows that after the treatment of aging microplastics by adding strong base respectively, after 8 days of culture, the OD of microalgae in the process of purifying the aging microplastics in 20, 100 and 200mg/L artificial simulation wastewater 680 0.553, 0.436 and 0.447, respectively, which were increased by 17.41% and decreased by 7.43% and 5.10%, respectively, compared to the control group.
FIG. 3 shows that OD of micro-algae in the micro-plastic aging process of purifying artificial simulated wastewater of 20, 100 and 200mg/L after micro-algae is cultured for 8 days after the micro-plastics aging treatment by adding strong acid respectively 680 0.551, 0.539 and 0.605, respectively, which are increased by 16.99%, 14.44% and 28.45%, respectively, compared to the control group.
After the aging micro-plastic treatment by adding strong base respectively and the culture for 8 days, the chlorophyll content of the microalgae in the aging micro-plastic process of purifying the artificial simulated wastewater of 20, 100 and 200mg/L is respectively 2.56mg/L, 2.14mg/L and 2.00mg/L, which is respectively increased by 19.63% and reduced by 0.09% and 6.54% compared with the control group.
After the treatment of the strong acid-aged microplastic is added and the culture is carried out for 8 days, the chlorophyll content of the microalgae in the process of purifying the aged microplastic in the artificial simulated wastewater of 20 mg/L, 100 mg/L and 200mg/L is respectively 2.47mg/L, 2.79mg/L and 2.81mg/L, which is respectively increased by 15.42% and reduced by 30.37% and 31.31% compared with the control group.
After the treatment of adding strong alkali to the aged microplastics respectively, and culturing for 8 days, the carotenoid contents of the microalgae in the processes of purifying the aged microplastics in 20, 100 and 200mg/L artificial simulated wastewater are respectively 0.74mg/L, 0.63mg/L and 0.61mg/L, which are respectively increased by 17.46% and reduced by 1.11% and 3.17% compared with the control group.
After the treatment of adding strong acid aged microplastic respectively and culturing for 8 days, the carotenoid contents of the microalgae in the processes of purifying the aged microplastic in 20, 100 and 200mg/L artificial simulated wastewater are respectively 0.77mg/L, 0.80mg/L and 0.82mg/L, which are respectively increased by 22.22%, 26.98% and 30.16% compared with the control group.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. A method for treating aged micro-plastic wastewater by using microalgae is characterized by comprising the following steps:
1) pre-culturing functional microalgae to reach logarithmic growth phase;
2) respectively aging the micro plastic wastewater by using strong acid or strong base;
3) and inoculating the functional microalgae cultured in the step 1) into the micro plastic wastewater respectively pretreated by strong acid or strong base in the step 2) to purify the high-concentration aged micro plastic wastewater.
2. The method for treating aged micro-plastic wastewater with microalgae of claim 1, wherein the functional microalgae in step 1) is Scenedesmus quadricauda or Chlorella vulgaris.
3. The method for treating the aged waste water of the micro-algae according to claim 1, wherein the pre-culturing conditions in the step 1) are illumination throughout the day, illumination intensity of 4800- 2 And N 2 Mixed gas of (2), CO 2 The volume fraction is 3% -5%, and the ventilation volume is 30-40 mL/min.
4. The method for treating aged micro-plastic wastewater by using microalgae according to claim 1, wherein the strong acid in step 2) is hydrochloric acid, sulfuric acid or nitric acid; the strong base is sodium hydroxide or potassium hydroxide.
5. The method for treating aged micro-plastic wastewater by using microalgae as claimed in claim 4, wherein the concentration of the strong acid or strong base is 5-20mol/L, and the treatment time is 24-48 h.
6. The method for treating aged micro-plastic wastewater by using microalgae as claimed in claim 4, wherein the concentration of the strong acid or the strong base is 10mol/L, and the treatment time is 48 h.
7. The method for treating the aged micro plastic wastewater by using the microalgae according to claim 1, wherein the micro plastic in the step 2) is polyethylene terephthalate with the size of 5-50 μm, and the concentration of the micro plastic after being treated by the strong acid or the strong base is 20-200 mg/L.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017130106A1 (en) * | 2016-01-25 | 2017-08-03 | Bio-P S.R.L. | Process for producing starch from microalgae |
| CN113943054A (en) * | 2021-10-25 | 2022-01-18 | 常熟理工学院 | Method for efficiently cleaning fluorine-containing micro-plastic in water body by using spirulina |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017130106A1 (en) * | 2016-01-25 | 2017-08-03 | Bio-P S.R.L. | Process for producing starch from microalgae |
| CN113943054A (en) * | 2021-10-25 | 2022-01-18 | 常熟理工学院 | Method for efficiently cleaning fluorine-containing micro-plastic in water body by using spirulina |
Non-Patent Citations (2)
| Title |
|---|
| CESAR CUNHA等: "Marine vs freshwater microalgae exopolymers as biosolutions to microplastics pollution", 《ENVIRONMENTAL POLLUTION》 * |
| 陈足音等: "老化微塑料对三角褐指藻生长及光合活性的影响", 《环境科学与技术》 * |
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