CN115109432B - Method for converting indigo by rolling wall breaking of bluish She Yin indole alkaloids - Google Patents
Method for converting indigo by rolling wall breaking of bluish She Yin indole alkaloids Download PDFInfo
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- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 title claims abstract description 67
- 235000000177 Indigofera tinctoria Nutrition 0.000 title claims abstract description 51
- 229940097275 indigo Drugs 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 43
- 238000005096 rolling process Methods 0.000 title claims abstract description 28
- 229930005303 indole alkaloid Natural products 0.000 title claims abstract description 16
- 150000002475 indoles Chemical class 0.000 title abstract description 5
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Chemical compound C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 claims abstract description 11
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 64
- 239000006260 foam Substances 0.000 claims description 52
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 31
- 238000001035 drying Methods 0.000 claims description 23
- 238000001816 cooling Methods 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 20
- 241001252483 Kalimeris Species 0.000 claims description 12
- 239000012535 impurity Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- -1 stems Substances 0.000 claims description 9
- 239000000428 dust Substances 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 3
- 239000012043 crude product Substances 0.000 claims description 3
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- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000007602 hot air drying Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims 6
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims 6
- 229930014456 matrine Natural products 0.000 claims 6
- 239000000126 substance Substances 0.000 abstract description 4
- 238000006911 enzymatic reaction Methods 0.000 abstract description 3
- 239000002243 precursor Substances 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 abstract description 2
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- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
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- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- SIKJAQJRHWYJAI-UHFFFAOYSA-N benzopyrrole Natural products C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 1
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- 229930182470 glycoside Natural products 0.000 description 1
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- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for converting indigo blue by rolling wall breaking of a mugwort She Yin indole alkaloid, and particularly relates to the field of plant processing. According to the invention, indole alkaloids are converted into indigo by a rolling wall breaking method, the cell structure of the indole alkaloids is destroyed by the action of mechanical external force of the blades, and precursor substances and enzymes are released respectively and subjected to enzymatic reaction.
Description
Technical Field
The invention relates to the technical field of plant processing, in particular to a method for twisting and breaking wall to convert indigo blue by using a bluish dogbane She Yin indole alkaloid.
Background
Indigo is one of the earliest natural pigments found in the world at present, the annual demand of the world is more than 25000 tons, and the market prospect is wide. The preparation process of the indigo and the application of the indigo in dyeing are recorded from ancient times in China, and the indigo is widely applied to dyeing of cotton fabrics, silk, wool and the like and can also be applied to food pigment. Although the process for synthesizing indigo is mature and the products occupy market main force at present, the sodium sulfite which is a potential carcinogen is needed in the synthesis process, and is decomposed into sulfuric acid and sulfurous acid in the process of preparing indigo, the treatment cost of the materials is high, and in order to reduce the additional cost of wastewater treatment, many factories directly discharge the carcinogenic wastes into rivers, so that serious harm is caused to the environment. Nowadays, health importance is becoming more and more appreciated, and natural indigo is therefore also becoming more and more important, and natural indigo is still an irreplaceable choice for the food industry.
At present, the indigo is prepared by adopting a traditional fermentation process in China, and the main process is as follows: after picking the indian kale, putting the stem leaves into a water pool, pressing the stem leaves into water with heavy objects, and soaking. Depending on the temperature, soaking for 3-8 days is required, when the raw materials are fully fermented, water turns dark green, the leaves of the bluish macranth are fished out and discarded, the leaf residues at this time produce a pungent smell, and then slowly poured into lime milk, with constant agitation until the liquid turns dark blue and there is a lot of purple foam floating on the surface, a process called "indigo beating", which is considered to be the most important process in the indigo production process. Finally, standing, discarding supernatant, generating a large amount of wastewater containing inorganic ions, and drying to obtain indigo coarse powder, wherein the precipitate at the bottom is indigo paste. Therefore, the process has the advantages of high content of indigo impurities, high labor intensity, low production efficiency, large wastewater amount, no utilization of waste residues and wastewater and large environmental pollution. Therefore, development of a modern technology which is fast, environment-friendly and suitable for industrial production is urgent.
Disclosure of Invention
In order to overcome the above-mentioned drawbacks of the prior art, an embodiment of the present invention provides a method for twisting and breaking wall of a basket She Yin indole alkaloid to convert indigo, so as to solve the problems set forth in the above-mentioned background art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for twisting wall-broken conversion indigo by using a bluish She Yin indole alkaloid comprises the following steps:
s1, preparing materials: screening fresh leaves of the Indian kalimeris herb, and cleaning to obtain clean fresh leaves;
s2, withering: placing the clean fresh leaves in the step S1 into a tray, paving the fresh leaves with the thickness of 0.5 cm-1.5 cm, and heating the fresh leaves by hot air at the temperature of 35-80 ℃ to obtain softened leaves after withering;
s3, cooling: cooling the softened leaves obtained in the step S2 to room temperature, stacking and standing for 0.5-2 h to balance water to obtain cooled leaves;
s4, enzyme deactivation: putting the cooling leaves obtained in the step S3 into a steamer, and carrying out steam fixation at 100 ℃ for 60-120S to obtain fixation leaves;
s5, breaking the wall: rolling, extruding and breaking the wall of the water-removing She Songru rolling machine prepared in the step S4, and collecting overflowed juice;
s6, floatation: placing the juice collected in the step S5 into a beaker, adding water, stirring, standing, and collecting upper foam; placing the foam into a separating funnel, continuously adding water to wash the foam, and repeating until the foam water washing liquid becomes colorless to obtain foam rich in indigo;
s7, drying: and (3) placing the foam collected in the step (S6) into a hot air drying box for drying to obtain an indigo crude product.
Further, the fresh leaves of the Indian kalimeris herb in the step S1 are leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the Indian kalimeris herb stems and leaves picked within 4-12 hours of the current day, and the water content is 70-85%.
Further, in the step S2, the hot air of a blast drying box is used for withering, and the water content of the softened fresh leaves of the Indian kalimeris herb is 50-60%.
Further, the stacking thickness of the cooling leaves in the step S3 is 10 cm-20 cm.
Further, the water content of the de-enzyming leaves in the step S4 is kept between 50% and 60%.
Further, in the step S5, the rolling time is 20-60 min, and the extrusion pressure is 70-150 kg.
Further, the water adding amount in the step S6 is 4-6 times of the mass of the fresh leaves, the stirring speed is 200-500 rpm, and the foam washing times are 3-5 times.
Further, the drying temperature in the step S7 is 50-60 ℃.
The invention has the technical effects and advantages that:
1. in the invention, indole alkaloids are converted into indigo by a rolling wall breaking method, and in fresh leaves of the Indian kalimeris herb, as precursor substances, namely, indole glycoside and related enzymes, which are related to the formation of the indigo are respectively stored in different parts of leaf cells, the fresh leaves are green; when the cell structure of the blade is destroyed under the action of mechanical external force, the precursor substances and the enzyme are released respectively and perform enzymatic reaction, and the enzyme has the characteristics of high efficiency and specificity, so that indigo can be rapidly generated under the condition of proper temperature and oxygen, and in the twisting process, the blade generates certain heat due to friction, thereby being beneficial to the enzymatic reaction and improving the reaction rate.
2. The invention adopts the fixation process, which aims at passivating oxidase existing in fresh leaves and inhibiting oxidation of alkaloid components, compared with the fixation process of Wen Sha with equal height, steam fixation is heated more uniformly, and the phenomena of black leaves, black spots and the like can not be generated due to scorching of leaves caused by local high temperature, and the conditions are easy to control. In addition, the penetrability of steam is stronger than that of hot air, so that the steam is easy to permeate into fresh leaves, and meanwhile, the pores of the leaves can be expanded, and substances in the leaves after the steam is deactivated are more easily dissolved out.
3. The invention utilizes the characteristic that the indigo is indissolvable in water, can rapidly remove a large amount of water-soluble impurities in the blades by a water washing mode, and the generated indigo floats on the water surface or in bubbles, and the purity of the product can reach 25-30% after drying.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a method for twisting wall-broken conversion of indigo by using a bluish She Yin indole alkaloid, which comprises the following steps:
s1, preparing materials: screening fresh leaves of the Indian kalimeris herb, and cleaning to obtain clean fresh leaves;
s2, withering: placing the clean fresh leaves in the step S1 into a tray, paving the fresh leaves with the thickness of 0.5 cm-1.5 cm, and heating the fresh leaves by hot air at the temperature of 35-80 ℃ to obtain softened leaves after withering;
s3, cooling: cooling the softened leaves obtained in the step S2 to room temperature, stacking and standing for 0.5-2 h to balance water to obtain cooled leaves;
s4, enzyme deactivation: putting the cooling leaves obtained in the step S3 into a steamer, and carrying out steam fixation at 100 ℃ for 60-120S to obtain fixation leaves;
s5, breaking the wall: rolling, extruding and breaking the wall of the water-removing She Songru rolling machine prepared in the step S4, and collecting overflowed juice;
s6, floatation: placing the juice collected in the step S5 into a beaker, adding water, stirring, standing, and collecting upper foam; placing the foam into a separating funnel, continuously adding water to wash the foam, and repeating until the foam water washing liquid becomes colorless to obtain foam rich in indigo;
s7, drying: and (3) placing the foam collected in the step (S6) into a hot air drying box for drying to obtain an indigo crude product.
In a preferred embodiment, the fresh leaves of the Indian kalimeris herb in the step S1 are leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the Indian kalimeris herb leaves picked within 4-12 hours of the day, and the water content is 70-85%.
In a preferred embodiment, the withering process in step S2 is performed using a forced air drying oven to wither the softened fresh leaves of kale with a moisture content of 50% to 60%.
In a preferred embodiment, the stacking thickness of the cooling leaves in step S3 is 10cm to 20cm.
In a preferred embodiment, the water content of the de-enzyming leaves in step S4 is kept between 50% and 60%.
In a preferred embodiment, the rolling time in step S5 is 20min to 60min and the pressing pressure is 70kg to 150kg.
In a preferred embodiment, the water addition amount in step S6 is 4 to 6 times the mass of the fresh leaves, the stirring speed is 200 to 500rpm, and the number of foam washes is 3 to 5.
In a preferred embodiment, the drying temperature in step S7 is 50 ℃ to 60 ℃.
Example 1
A method for twisting wall-broken conversion indigo by using a bluish She Yin indole alkaloid comprises the following steps:
s1, taking 1000g of leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the stems and leaves of the bluish dogwood picked within 4-12 hours on the same day, wherein the water content is 78%, and preparing clean bluish dogwood fresh leaves;
s2, placing the clean fresh leaves of the Chinese kale obtained in the step S1 into a tray, paving the fresh leaves to be 0.5cm thick, and then placing the fresh leaves into a blast drying box, wherein the temperature of hot air is 35 ℃, and the water content of the softened leaves is 58.6%;
s3, stacking, standing and cooling the softened leaves prepared in the step S2 at room temperature for 2 hours, wherein the thickness is 10cm, balancing the moisture, and the moisture content of the cooled leaves is 58.3%;
s4, placing the cooling leaves obtained in the step S3 into a steamer, setting the temperature to be 100 ℃, and using steam to deactivate for 60 seconds, wherein the water content of the deactivated leaves is 58%;
s5, placing the de-enzyming leaves prepared in the step S4 into a material groove of a rolling machine, paving the material groove with the thickness of 10cm, compacting the leaves by rotating a material groove cover at the rotating speed of 60rpm for 20min, and then collecting generated foam and juice into a barrel for later use; repeatedly rolling the rolled de-enzymed leaves in a single screw squeezer under the pressure of 70kg, adding 4L of water in a rolling process for each time, adding 1L of water each time, collecting overflowed juice and combining the overflowed juice with juice in a barrel;
s6, stirring the juice combined in the step S5 by using a direct current stirrer, controlling the rotating speed to be 200rpm and the time to be 10min, collecting generated foam after stirring is finished, putting the foam into a new barrel, adding 1L of water, continuously stirring and collecting foam according to the previous condition, repeating the process for 3 times until a water layer is colorless, discarding liquid, and collecting foam rich in indigo;
and S7, placing the foam obtained in the step S6 into an oven, setting the temperature to be 50 ℃, and drying to obtain powder rich in alkaloids such as indigo.
Example 2
A method for twisting wall-broken conversion indigo by using a bluish She Yin indole alkaloid comprises the following steps:
s1, taking 1000g of leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the stems and leaves of the bluish dogwood picked within 4-12 hours on the same day, wherein the water content is 78%, and preparing clean bluish dogwood fresh leaves;
s2, placing the clean fresh leaves of the Chinese kale obtained in the step S1 into a tray, paving the fresh leaves to be 1.5cm thick, and then placing the fresh leaves into a blast drying box, wherein the temperature of hot air is 80 ℃, and the water content of the softened leaves is 58.3%;
s3, stacking, standing and cooling the softened leaves prepared in the step S2 at room temperature for 2 hours, wherein the thickness is 20cm, balancing the moisture, and the moisture content of the cooled leaves is 58.1%;
s4, placing the cooling leaves obtained in the step S3 into a steamer, setting the temperature to be 100 ℃, and using steam to deactivate for 120S, wherein the water content of the deactivated leaves is 56%;
s5, placing the de-enzyming leaves prepared in the step S4 into a material groove of a rolling machine, paving the material groove with the thickness of 10cm, compacting the leaves by rotating a material groove cover at the rotating speed of 60rpm for 60min, and then collecting generated foam and juice into a barrel for later use; repeatedly rolling the rolled de-enzymed leaves in a single screw squeezer under the pressure of 150kg, adding 4L of water in a rolling process for each time, adding 1L of water each time, collecting overflowed juice and combining the overflowed juice with juice in a barrel;
s6, stirring the juice combined in the step S5 by using a direct current stirrer, controlling the rotating speed to be 400rpm and the time to be 10min, collecting generated foam after stirring is finished, putting the foam into a new barrel, adding 1.5L of water, continuously stirring and collecting foam according to the previous condition, repeating the process for 5 times until a water layer is colorless, discarding liquid, and collecting foam rich in indigo;
and S7, placing the foam obtained in the step S6 into an oven, setting the temperature to be 60 ℃, and drying to obtain powder rich in alkaloids such as indigo.
Example 3
A method for twisting wall-broken conversion indigo by using a bluish She Yin indole alkaloid comprises the following steps:
s1, taking 1000g of leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the stems and leaves of the bluish dogwood picked within 4-12 hours on the same day, wherein the water content is 78%, and preparing clean bluish dogwood fresh leaves;
s2, placing the clean fresh leaves of the Chinese kale obtained in the step S1 into a tray, paving the fresh leaves to be 1cm thick, and then placing the fresh leaves into a blast drying box, wherein the temperature of hot air is 55 ℃, and the water content of the softened leaves is 53.4%;
s3, stacking, standing and cooling the softened leaves prepared in the step S2 at room temperature for 2 hours, wherein the thickness is 20cm, balancing the moisture, and the moisture content of the cooled leaves is 53.1%;
s4, placing the cooling leaves obtained in the step S3 into a steamer, setting the temperature to be 100 ℃, and using steam to deactivate for 120S, wherein the water content of the deactivated leaves is 57%;
s5, placing the de-enzyming leaves prepared in the step S4 into a material groove of a rolling machine, paving the material groove with the thickness of 10cm, compacting the leaves by rotating a material groove cover at the rotating speed of 60rpm for 60min, and then collecting generated foam and juice into a barrel for later use; repeatedly rolling the rolled de-enzymed leaves in a single screw squeezer under the pressure of 100kg, adding 4L of water in a rolling process for each time, adding 1L of water each time, collecting overflowed juice and combining the overflowed juice with juice in a barrel;
s6, stirring the juice combined in the step S5 by using a direct current stirrer, controlling the rotating speed to be 300rpm and the time to be 10min, collecting generated foam after stirring is finished, putting the foam into a new barrel, adding 1.5L of water, continuously stirring and collecting foam according to the previous condition, repeating the process for 5 times until a water layer is colorless, discarding liquid, and collecting foam rich in indigo;
and S7, placing the foam obtained in the step S6 into an oven, setting the temperature to 55 ℃, and drying to obtain powder rich in alkaloids such as indigo.
Example 4
A method for twisting wall-broken conversion indigo by using a bluish She Yin indole alkaloid comprises the following steps:
s1, taking 1000g of leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the stems and leaves of the bluish dogwood picked within 4-12 hours on the same day, wherein the water content is 78%, and preparing clean bluish dogwood fresh leaves;
s2, placing the clean fresh leaves of the Chinese kale obtained in the step S1 into a tray, paving the fresh leaves to be 1cm thick, and then placing the fresh leaves into a blast drying box, wherein the temperature of hot air is 80 ℃, and the water content of the softened leaves is 56.6%;
s3, stacking, standing and cooling the softened leaves prepared in the step S2 at room temperature for 2 hours, wherein the thickness is 20cm, balancing the moisture, and the moisture content of the cooled leaves is 56.2%;
s4, placing the cooling leaves obtained in the step S3 into a steamer, setting the temperature to be 100 ℃, and using steam to deactivate for 120S, wherein the water content of the deactivated leaves is 54%;
s5, placing the de-enzyming leaves prepared in the step S4 into a material groove of a rolling machine, paving the material groove with the thickness of 20cm, compacting the leaves by rotating a material groove cover at the rotating speed of 60rpm for 60min, and then collecting generated foam and juice into a barrel for later use; repeatedly rolling the rolled de-enzymed leaves in a single screw squeezer under the pressure of 150kg, adding 4L of water in a rolling process for each time, adding 1L of water each time, collecting overflowed juice and combining the overflowed juice with juice in a barrel;
s6, stirring the juice combined in the step S5 by using a direct current stirrer, controlling the rotating speed to be 500rpm and the time to be 10min, collecting generated foam after stirring is finished, putting the foam into a new barrel, adding 1.5L of water, continuously stirring and collecting foam according to the previous condition, repeating the process for 5 times until a water layer is colorless, discarding liquid, and collecting foam rich in indigo;
and S7, placing the foam obtained in the step S6 into an oven, setting the temperature to be 60 ℃, and drying to obtain powder rich in alkaloids such as indigo.
Example 5
A method for twisting wall-broken conversion indigo by using a bluish She Yin indole alkaloid comprises the following steps:
s1, taking 1000g of leaves which are not damaged after removing impurities such as stems, dust and mud and the like from the stems and leaves of the bluish dogwood picked within 4-12 hours on the same day, wherein the water content is 78%, and preparing clean bluish dogwood fresh leaves;
s2, placing the clean fresh leaves of the Chinese kale obtained in the step S1 into a tray, paving the fresh leaves to be 0.5cm thick, and then placing the fresh leaves into a blast drying box, wherein the temperature of hot air is 80 ℃, and the water content of the softened leaves is 54.8%;
s3, stacking, standing and cooling the softened leaves prepared in the step S2 at room temperature for 2 hours, wherein the thickness is 1cm, balancing the moisture, and the moisture content of the cooled leaves is 54.3%;
s4, placing the cooling leaves obtained in the step S3 into a steamer, setting the temperature to be 100 ℃, and using steam to deactivate for 120S, wherein the water content of the deactivated leaves is 53%;
s5, placing the de-enzyming leaves prepared in the step S4 into a material groove of a rolling machine, paving the material groove with the thickness of 10cm, compacting the leaves by rotating a material groove cover at the rotating speed of 60rpm for 60min, and then collecting generated foam and juice into a barrel for later use; repeatedly rolling the rolled de-enzymed leaves in a single screw squeezer under the pressure of 150kg, adding 4L of water in a rolling process for each time, adding 1L of water each time, collecting overflowed juice and combining the overflowed juice with juice in a barrel;
s6, stirring the juice combined in the step S5 by using a direct current stirrer, controlling the rotating speed to be 500rpm and the time to be 10min, collecting generated foam after stirring is finished, putting the foam into a new barrel, adding 1L of water, continuously stirring and collecting foam according to the previous condition, repeating the process for 5 times until a water layer is colorless, discarding liquid, and collecting foam rich in indigo;
and S7, placing the foam obtained in the step S6 into an oven, setting the temperature to be 60 ℃, and drying to obtain powder rich in alkaloids such as indigo.
The above examples prepared powder 1-5 rich in indigo alkaloid and so on with the same weight respectively to prepare samples, then to carry out comparison and analysis, and under the same conditions, to carry out evaluation and absorption effect monitoring on two indexes of powder quality, indigo content and yield in the powder rich in indigo alkaloid and so on; the monitoring results are shown in table 1 below:
table 1 table of suction effect for samples made with different amounts of materials by weight for examples 1-5 under the same conditions.
| Project | Powder quality (mg) | Indigo content (%) | Yield (%) |
| Example 1 | 20 | 26.4 | 49.8 |
| Example 2 | 20 | 25.8 | 49.1 |
| Example 3 | 20 | 25.4 | 48.9 |
| Example 4 | 20 | 25.1 | 48.6 |
| Example 5 | 20 | 26.0 | 49.3 |
From the above, it can be seen from the data in examples 1-3 that example 1 is the best mode.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (7)
1. The method for twisting and breaking wall of the bluish She Yin indole alkaloid to convert the indigo is characterized by comprising the following steps of:
s1, preparing materials: screening fresh leaves of the Indian kalimeris herb, and cleaning to obtain clean fresh leaves;
s2, withering: placing the clean fresh leaves in the step S1 into a tray, paving the fresh leaves with the thickness of 0.5 cm-1.5 cm, and heating the fresh leaves by hot air at the temperature of 35-80 ℃ to obtain softened leaves after withering;
s3, cooling: cooling the softened leaves obtained in the step S2 to room temperature, stacking and standing for 0.5-2 h to balance water to obtain cooled leaves;
s4, enzyme deactivation: putting the cooling leaves obtained in the step S3 into a steamer, and carrying out steam fixation at 100 ℃ for 60-120S to obtain fixation leaves;
s5, breaking the wall: rolling, extruding and breaking the wall of the water-removing She Songru rolling machine prepared in the step S4, and collecting overflowed juice;
s6, floatation: placing the juice collected in the step S5 into a beaker, adding water, stirring, standing, and collecting upper foam; placing the foam into a separating funnel, continuously adding water to wash the foam, and repeating until the foam water washing liquid becomes colorless to obtain foam rich in indigo;
s7, drying: and (3) placing the foam collected in the step (S6) into a hot air drying box for drying to obtain an indigo crude product.
2. The method for twisting and breaking wall to convert indigo blue by using the matrine She Yin as claimed in claim 1, wherein the method comprises the following steps: the fresh leaves of the Indian kalimeris herb in the step S1 are leaves which are not damaged after the leaves of the Indian kalimeris herb picked within 4-12 hours of the current day are subjected to the removal of impurities such as stems, dust and mud, and the like, and the water content is 70-85%.
3. The method for twisting and breaking wall to convert indigo blue by using the matrine She Yin as claimed in claim 1, wherein the method comprises the following steps: and in the step S2, the hot air of a blast drying box is used for withering, and the water content of the softened fresh leaves of the Indian kalimeris herb is 50-60%.
4. The method for twisting and breaking wall to convert indigo blue by using the matrine She Yin as claimed in claim 1, wherein the method comprises the following steps: the stacking thickness of the cooling leaves in the step S3 is 10 cm-20 cm.
5. The method for twisting and breaking wall to convert indigo blue by using the matrine She Yin as claimed in claim 1, wherein the method comprises the following steps: the water content of the de-enzyming leaves in the step S4 is kept between 50 and 60 percent.
6. The method for twisting and breaking wall to convert indigo blue by using the matrine She Yin as claimed in claim 1, wherein the method comprises the following steps: the water adding amount in the step S6 is 4-6 times of the mass of the fresh leaves, the stirring speed is 200-500 rpm, and the foam washing times are 3-5 times.
7. The method for twisting and breaking wall to convert indigo blue by using the matrine She Yin as claimed in claim 1, wherein the method comprises the following steps: the drying temperature in the step S7 is 50-60 ℃.
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| CN1970642A (en) * | 2006-11-10 | 2007-05-30 | 中国农业大学 | Biological synthesis method for dark indigo |
| CN102247415A (en) * | 2010-05-21 | 2011-11-23 | 成都中医药大学 | Method for preparing indigo naturalis |
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| CN1970642A (en) * | 2006-11-10 | 2007-05-30 | 中国农业大学 | Biological synthesis method for dark indigo |
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| CN105178061A (en) * | 2015-09-28 | 2015-12-23 | 江苏丹毛纺织股份有限公司 | Technological method for dyeing wool tops through plant indigo dye |
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