CN115106142A - Multichannel interval-adjustable liquid transfer device and cup separating system - Google Patents
Multichannel interval-adjustable liquid transfer device and cup separating system Download PDFInfo
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- CN115106142A CN115106142A CN202210798159.7A CN202210798159A CN115106142A CN 115106142 A CN115106142 A CN 115106142A CN 202210798159 A CN202210798159 A CN 202210798159A CN 115106142 A CN115106142 A CN 115106142A
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- 239000007788 liquid Substances 0.000 title claims abstract description 54
- 230000007246 mechanism Effects 0.000 claims abstract description 39
- 239000011159 matrix material Substances 0.000 claims abstract description 15
- 238000007599 discharging Methods 0.000 claims abstract description 4
- 230000005540 biological transmission Effects 0.000 claims description 12
- 210000000056 organ Anatomy 0.000 claims description 8
- 229920001971 elastomer Polymers 0.000 claims description 6
- 239000000806 elastomer Substances 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 5
- 230000009467 reduction Effects 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 15
- 230000008569 process Effects 0.000 description 12
- 150000007523 nucleic acids Chemical class 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
- 108020004707 nucleic acids Proteins 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000012943 hotmelt Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0275—Interchangeable or disposable dispensing tips
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
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- Hematology (AREA)
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a multichannel spacing-adjustable pipetting device, which comprises an adjusting mechanism and a pipetting mechanism, wherein the adjusting mechanism comprises an elastic body and an inflation and deflation device; the invention utilizes the air charging and discharging device to charge and discharge air to the elastic body, controls the matrix formed by arranging a plurality of rigid structures to be amplified or reduced in equal proportion, can quickly adjust the space between a plurality of rows of pipette guns and a large number of pipette guns, and meets the requirements of the space between test tubes on the sampling tube rack and the space between holes on the pipette plate, thereby solving the problem that the space adjustment of the plurality of rows of pipette guns can not be realized in the existing multi-channel pipette guns. The multichannel adjustable-interval liquid transfer device is novel and simple in structure, convenient to operate and beneficial to greatly improving the efficiency of a cup separating system. The invention also provides a cup separating system which comprises the transfer mechanism and the multi-channel liquid transferring device with the adjustable space, can realize the quick adjustment of the space of the liquid transferring guns in multiple rows and in large batch, and greatly improves the efficiency of the cup separating system.
Description
Technical Field
The invention relates to the field of medical instruments, in particular to a multichannel spacing-adjustable liquid transfer device and a cup separating system.
Background
The virus nucleic acid detection needs to carry out the processes of sample collection, sample pretreatment, virus nucleic acid extraction, nucleic acid detection, result interpretation and the like. In this case, the sample needs to be inactivated and subjected to plate transfer before nucleic acid extraction. The conventional work of rotating the plate after inactivation needs manual operation, which comprises a series of complicated works of information checking, cover opening, liquid transferring and cover closing, and the process is called as 'cup separating' for short. For improving the nucleic acid detection flux, it is important to accelerate the speed of the cup separation system. In order to further improve the detection throughput and reduce the workload of medical staff, the cup separating system is usually completed in an automatic manner, i.e., an automatic cup separating system. Wherein, the pipetting step is the most time-consuming ring in the operation process of the cup separating system.
The liquid transfer method comprises the following steps that a manipulator is generally used for carrying out liquid transfer, a liquid transfer gun is clamped to be moved to the position above a certain uncapped sampling tube, the head of the liquid transfer gun is immersed into the sampling tube through the feeding motion in the vertical direction, a negative pressure is applied to the air pipe communicated with the liquid transfer gun, so that the liquid transfer gun absorbs quantitative liquid to be tested in the sampling tube, the liquid transfer gun is moved to the position above a liquid transfer plate through a mechanical handle, and the negative pressure is removed to inject the liquid to be tested into a hole corresponding to the liquid transfer plate, so that the liquid transfer operation is completed.
In order to further increase the throughput of the pipetting step and the efficiency of nucleic acid detection, it is necessary to use a plurality of pipetting guns simultaneously to accomplish the above-mentioned work, i.e., a multi-channel pipetting gun. However, since the pitch of the nucleic acid sampling tube racks (5-10ml, 20mm, and about 30 mm) is different from the pitch (9mm) of the conventional 96-well plate, pipetting cannot be performed only by mounting a plurality of pipette tips at fixed positions on the robot arm, but the multichannel pipette gun is required to be adjustable while keeping the pitch the same.
At present, there are mainly two ways to realize that the interval of multichannel pipetting gun is adjustable: one is by some mechanical structure, such as a spring or linkage; the other is realized by adding a motor. However, the application range of the two methods is generally a single-row pipette structure, and once the liquid-transfering guns are expanded to a multi-row pipette structure, a large number of pipette guns cause the problems of too many parts in the adjusting mechanism, too complex structure, strong instability in use and the like. Therefore, a multi-channel liquid transferring device with adjustable spacing is needed to be designed, wherein the device is simple in structure, high in use stability and applicable to spacing adjustment of multiple rows of liquid transferring guns.
Disclosure of Invention
The invention aims to provide a multichannel spacing-adjustable pipetting device and a cup separating system, and aims to solve the problem that the spacing adjustment of a plurality of rows of pipetting guns cannot be realized in the conventional multichannel pipetting gun.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a multichannel spacing-adjustable pipetting device, which comprises:
the adjusting mechanism comprises an elastic body and an air charging and discharging device, a plurality of rigid structures distributed in a matrix are embedded in the elastic body, and the geometric center of the outer contour of the matrix is superposed with the geometric center of the outer contour of the elastic body; the inflation and deflation device is connected with the elastic body and can inflate or deflate the elastic body to realize the equal proportional amplification or reduction of the space between any two adjacent rows of the rigid structures and the space between any two adjacent columns of the rigid structures in the matrix;
the liquid suction mechanism comprises a plurality of liquid transferring guns and a negative pressure liquid suction device, the liquid transferring guns are arranged on the rigid structure, and the liquid transferring guns are arranged in one-to-one correspondence with the rigid structure; and any one of the liquid-transferring guns is provided with a gas transmission channel, and the gas transmission channel is connected with the negative pressure liquid-absorbing device.
Optionally, the elastic body is a rectangular elastic air bag.
Optionally, the rigid structure is a rigid pipe fitting, any rigid pipe fitting penetrates through the top surface and the bottom surface of the rectangular elastic air bag, the outer wall of any rigid pipe fitting is hermetically connected with the rectangular elastic air bag, and a radial convex ring is arranged at the bottom of the inner wall of any rigid pipe fitting to fix the liquid-transferring gun.
Optionally, any one side surface of the rectangular elastic airbag is set to be an extensible organ structure surface, and any one organ structure surface can be extended and retracted along a direction perpendicular to the rigid pipe fitting.
Optionally, the apparatus further comprises a guide mechanism, wherein the guide mechanism comprises:
the supporting plate is arranged above the rectangular elastic air bag, two pairs of sliding rails are arranged on the supporting plate, two sliding rails in each pair of sliding rails are positioned on the same straight line, and the projections of the two pairs of sliding rails on the rectangular elastic air bag are respectively superposed with two diagonal lines of the rectangular elastic air bag;
the sliding blocks are in sliding fit with the sliding rails, and each sliding rail is provided with one sliding block in a sliding mode;
the top end of the connecting rod is connected with the sliding block, and the bottom end of the connecting rod is connected with the corner of the rectangular elastic air bag.
Optionally, the support plate is a rectangular support plate.
Optionally, four corners of the rectangular elastic airbag are provided with connecting lugs, and the connecting lugs are connected with the bottom end of the connecting rod.
Optionally, the connecting rod is a stud bolt, and two ends of the stud bolt respectively penetrate through the sliding block and the connecting lug and are in locking fit with the nut.
Optionally, the inflation/deflation device comprises a gas pipe and a gas source, one end of the gas pipe is connected with the elastic body, and the other end of the gas pipe is connected with the gas source.
Optionally, the negative pressure liquid suction device is a negative pressure air source, and each air transmission channel is connected with the negative pressure air source.
Meanwhile, the invention provides a cup separation system which comprises a transfer mechanism and the multi-channel liquid transfer device with the adjustable space, wherein the transfer mechanism is used for lifting and transferring the multi-channel liquid transfer device with the adjustable space.
Optionally, the transfer mechanism is a robot.
Compared with the prior art, the invention has the following technical effects:
the multichannel pipetting device with the adjustable interval utilizes the inflation and deflation device to inflate and deflate the elastic body, controls the matrix formed by arranging the rigid structures to be enlarged or reduced in equal proportion, can quickly adjust the intervals of a plurality of rows and a large number of pipetting guns, meets the requirement of the interval between test tubes on the sampling tube rack and the interval between holes on the pipetting plate, and solves the problem that the interval adjustment of the plurality of rows of pipetting guns cannot be realized in the conventional multichannel pipetting gun. The multichannel adjustable-interval liquid transfer device is novel and simple in structure, convenient to operate and beneficial to greatly improving the efficiency of a cup separating system.
In addition, the invention provides a cup separating system, which comprises a transfer mechanism and the multi-channel adjustable-interval pipetting device, wherein the transfer mechanism is used for lifting and transferring the multi-channel adjustable-interval pipetting device. This branch cup system can realize multirow, big interval quick adjustment of pipetting gun in batches through setting up foretell multichannel interval adjustable pipetting device, has improved branch cup system's efficiency greatly.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an elastomer in an inflated expanded state in a multi-channel adjustable-pitch pipetting device according to an embodiment of the invention;
FIG. 2 is a schematic structural diagram of an elastomer in a deflated state in the multi-channel adjustable-pitch pipetting device disclosed by the embodiment of the invention;
FIG. 3 is a schematic structural diagram of a guide mechanism according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a fluid aspirating mechanism disclosed in an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of an adjusting mechanism according to an embodiment of the present invention;
FIG. 6 is a graph of the results of a finite element analysis of the elastomer inflation and deflation process disclosed in the embodiments of the present invention.
Wherein the reference numerals are:
100. a multi-channel spacing adjustable pipetting device;
1. an adjustment mechanism; 11. an elastomer; 111. an organ structure surface; 12. a rigid structure; 121. a radial convex ring; 13. a gas delivery pipe;
2. a liquid suction mechanism; 21. a liquid transferring gun; 211. a gun head; 22. a gas transmission channel;
3. a guide mechanism; 31. a support plate; 32. a slide rail; 33. a slider; 34. a connecting rod; 35. a nut;
4. and (5) connecting lugs.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
One of the purposes of the invention is to provide a multi-channel liquid transfer device with adjustable space, which is used for solving the problem that the space adjustment of a plurality of rows of liquid transfer guns cannot be realized in the existing multi-channel liquid transfer gun.
The invention also aims to provide a cup separating system which comprises a transfer mechanism and the multi-channel liquid transferring device with the adjustable spacing, and can solve the problem of low working efficiency of the existing cup separating system.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example one
As shown in fig. 1 to 2, the present embodiment provides a multichannel spacing adjustable pipetting device 100, which mainly includes an adjusting mechanism 1 and a pipetting mechanism 2, wherein the adjusting mechanism 1 includes an elastic body 11 and a gas charging and discharging device, a plurality of rigid structures 12 distributed in a matrix are embedded on the elastic body 11, and the geometric center of the outer contour of the matrix coincides with the geometric center of the outer contour of the elastic body 11; the inflation and deflation device is connected with the elastic body 11, and can inflate or deflate the elastic body 11 to realize the equal proportional amplification or reduction of the distance between any two adjacent rows of rigid structures 12 and the distance between any two adjacent columns of rigid structures 12 in the matrix; the liquid suction mechanism 2 comprises a plurality of liquid transferring guns 21 and a negative pressure liquid suction device, the liquid transferring guns 21 are arranged on the rigid structure 12, and the liquid transferring guns 21 are arranged corresponding to the rigid structure 12 one by one; any liquid-transfering gun 21 is provided with a gas-transfering channel 22, and the gas-transfering channel 22 is connected with a negative pressure liquid-transfering device. The multichannel spacing adjustable pipetting device 100 specifically utilizes the elastic body 11 to perform horizontal (transverse) equal-proportion amplification or reduction in the process of inflation or deflation, and further drives the multiple rows of pipetting guns placed therein to perform the equal-proportion amplification or reduction of the row spacing and the column spacing, so as to realize the spacing adjustment of the multiple rows of multichannel pipetting guns.
In the present embodiment, the elastic body 11 is preferably a rectangular elastic bladder. Rigid structure 12 is the rigidity pipe fitting, and arbitrary rigidity pipe fitting all runs through the top surface and the bottom surface setting of rectangle elasticity gasbag, and the outer wall of arbitrary rigidity pipe fitting all with rectangle elasticity gasbag sealing connection, the setting of rigidity pipe fitting does not influence the intercommunication of the inside air cavity of rectangle elasticity gasbag. The bottom of the inner wall of any one rigid pipe fitting is provided with a radial convex ring 121 to form a stepped hole structure, so that a shaft shoulder formed by the radial convex ring 121 supports the liquid-transfering gun 21, and the liquid-transfering gun 21 is axially fixed in the rigid pipe fitting. The rectangular elastic air bag deforms in equal proportion in the processes of inflation and deflation, and the time of the distance is kept equal; the rigid structure 12 will not deform due to the change of pressure during the process of inflating and deflating the rectangular elastic air bag.
In this embodiment, any side surface of the rectangular elastic airbag, that is, any side surface around the rectangular elastic airbag except the top surface and the bottom surface, is set as the retractable accordion structure surface 111, and any accordion structure surface 111 can be retracted in a direction perpendicular to the rigid pipe. The rectangular elastic airbag of the present embodiment has the accordion-structured surfaces 111 on the outer peripheral surface thereof, and thus, when expanded or contracted, the rectangular elastic airbag is not substantially vertically deformed but only laterally deformed, with the axial direction of the rigid pipe member being the vertical deformation direction and the direction perpendicular to the vertical deformation direction being the lateral deformation direction.
In this embodiment, the airbag device further comprises a guide mechanism 3, the guide mechanism 3 comprises a support plate 31, a slider 33 and a connecting rod 34, the support plate 31 is disposed above the rectangular elastic airbag, two pairs of slide rails 32 are disposed on the support plate 31, two slide rails 32 of each pair of slide rails 32 are located on the same straight line, and projections of the two pairs of slide rails 32 on the rectangular elastic airbag are respectively overlapped with two diagonal lines of the rectangular elastic airbag, that is, the two pairs of slide rails 32 are arranged according to the two diagonal lines of the rectangular elastic airbag; the sliding blocks 33 are in sliding fit with the sliding rails 32, and each sliding rail 32 is provided with one sliding block 33 in a sliding way; the top end of the connecting rod 34 is connected with the sliding block 33, and the bottom end is connected with the corner of the rectangular elastic air bag. Among them, the support plate 31 is preferably a rectangular support plate 31, and the center of the rectangular support plate 31 is coaxially arranged with the center of the rectangular elastic bag. The guide mechanism 3 is used for further limiting the deformation of the rectangular elastic air bag, ensuring the equal proportion deformation of the rectangular elastic air bag, and simultaneously realizing the axial fixing and guiding functions of the rectangular elastic air bag, wherein the axial fixing is used for preventing the rectangular elastic air bag from falling off, and the guiding is used for enabling the deformation of the rectangular elastic air bag to be more controllable and stable.
As a further preferred scheme, two ends of the sliding rail 32 may be provided with a limiting structure, and when the sliding block 33 slides to the limiting positions of the two ends, the two limiting structures correspond to the two adjustable intervals.
In this embodiment, four corners of the rectangular elastic airbag are provided with the connection lugs 4, and the connection lugs 4 are connected with the bottom ends of the connection rods 34. Preferably, the connecting rod 34 is a stud bolt, and two ends of the stud bolt are respectively arranged through the sliding block 33 and the connecting lug 4 and are locked and matched with the nut 35.
In this embodiment, the inflation and deflation device includes an air pipe 13 and an air source, one end of the air pipe 13 is connected with the elastic body 11, and the other end is connected with the air source. Preferably, the air pipe 13 and the elastic body 11 are hermetically connected by hot melt adhesion or by arranging a sealing gasket.
In this embodiment, the negative pressure liquid suction device is preferably a negative pressure air source, the air transmission channel 22 of each liquid transfer gun 21 is connected to the negative pressure air source, each air transmission channel 22 may correspond to one negative pressure air source, or a plurality of air transmission channels 22 share one negative pressure air source.
In this embodiment, the elastic body 11 is highly elastic, so that it is deformed rapidly and significantly during the inflation and deflation processes. The elastomer 11 is required to have high tensile resilience and high elongation at break, and natural latex can be selected as a manufacturing material thereof. In order to clarify the principle of the elastic body 11 for realizing the equidistant change and clarify the application range of the equidistant change, the finite element analysis adopts a rectangle for analysis. As shown in fig. 6, finite element analysis is performed in COMSOL on the theory that the elastic body 11 deforms at equal intervals in the inflation and deflation process, in the structure on the left side in fig. 6, a tensile force which is perpendicular to the corresponding side and outward and has equal unit length is applied to each side, and the center is set as a fixed point, so that the obtained expanded structure is shown in the structure on the right side in fig. 6, and it can be found that the intervals between the rigid structures 12 in the figure are almost expanded at equal proportion, so that it can be proved that as long as the arrangement center of the rigid structure 12 array is coincident with the geometric center of the boundary graph of the elastic body 11, the elastic body 11 can maintain deformation at equal intervals, and the difficulty in designing the elastic body 11 is further reduced.
In the embodiment, the organ structure surface 111 (also called as a folding structure) can ensure that the dimension of the rectangular elastic air bag is changed only in the transverse direction in the process of inflation and deflation; the rectangular elastic air bag can ensure that the intervals of the liquid suction mechanisms are equal, and the air bag is reduced or expanded in equal proportion when the pressure intensity of the air bag is changed; the guide mechanism can improve the stability of the rectangular elastic air bag when the rectangular elastic air bag deforms, and the rectangular elastic air bag can be adjusted to switch between two set pressure intensities when the rectangular elastic air bag is used. The multichannel pipetting device 100 with adjustable spacing of the embodiment has a simple structure, can adjust the spacing of a plurality of rows and a plurality of pipetting guns at the same time, and keeps the spacing consistent. The following takes the rigid structures 12 on the rectangular elastic air bag as an example, which are uniformly distributed in a 5 × 5 matrix, and the working principle of the multi-channel interval adjustable pipetting device 100 of the present embodiment is specifically described, wherein the multi-channel interval adjustable pipetting device 100 is mainly used to adjust the intervals between pipetting guns distributed in a 5 × 5 matrix from the test tube row intervals on the sampling tube rack to the intervals between 96 well plates (the 96 well plate is one of the pipetting plates, which is the prior art), and only 2 stable intervals are required. The specific operation steps and flow are as follows:
taking the deflation process as an example, in the initial state, the rectangular elastic air bag is filled with gas (higher pressure), the organ structure surface 111 is supported without folds, by designing the structure size and the interval size of the rigid structures 12 on the rectangular elastic air bag, the row interval of the rigid structures 12 is the test tube row interval (assumed to be 20mm) on the sampling tube rack at this time, the interval between two adjacent rigid structures 12 in each row of rigid structures 12 is equal to the interval between two adjacent test tubes in each row of test tubes on the sampling tube rack, the interval of the pipetting gun 21 installed in the rigid structure 12 is also the same as the two intervals, and the sliding block 33 is at the starting point position (outer edge position) of the sliding rail 32 at this time. Integrally moving the multi-channel interval adjustable pipetting device 100 through a corresponding moving auxiliary device, such as a manipulator, so that the gun heads 211 of the plurality of pipetting guns 21 extend into the corresponding test tubes, applying negative pressure to the corresponding pipetting guns 21 through the gas transmission channels 22, so that the pipetting guns 21 absorb liquid to be tested in the corresponding test tubes, and then moving the whole multi-channel interval adjustable pipetting device 100 upwards to an initial position; meanwhile, the air pipe 13 extracts the air in the rectangular elastic air bag, so that the rectangular elastic air bag transversely shrinks in equal proportion, each organ structure surface 111 begins to fold according to the original folding surface, the matrix formed by the rigid structure 12 is reduced in equal proportion, the distance between the pipette guns 21 begins to be reduced in equal proportion until the row distance of the pipette guns 21 and the distance between two adjacent pipette guns 21 in each row of pipette guns 21 are respectively the same as the row distance (such as 9mm) and the column distance of the holes on the 96-hole plate under a certain pressure (at the moment, the general slide block 33 runs to the innermost end of the slide rail 32), at the moment, the whole multi-channel distance adjustable pipetting device 100 is moved downwards, the pipette guns 21 extend into the corresponding holes of the 96-hole plate, then the negative pressure in the gas transmission channel 22 is removed, so that the liquid to be detected stored in the liquid-transferring gun 21 is released into the corresponding hole of the 96-well plate, and the liquid-transferring process is completed.
In the above, the pipette device 100 with adjustable multi-channel spacing adjusts the spacing of the pipette guns distributed in a 5 × 5 matrix from the test tube row spacing on the sampling tube rack to the spacing of the 96-well plate, and only 2 stable spacings are needed, so that the specific pressures of two different spacings can be tested in advance, and the pressure can be switched between the two values only by controlling the pressure during use. The whole adjusting mechanism may have the problem of insufficient precision, and the rectangular elastic air bag can complete the distance adjusting work because the diameter of the hole of the 96-hole plate is 8.5mm and the requirement on the precision is not high. Compared with the prior art, the adjustable pipetting device 100 of multichannel interval of this technical scheme can make multirow, a large amount of pipetting gun's interval adjust fast, and the sample tube support only needs once to accomplish 25 pipetting guns 21 or more pipetting guns 21's of pipetting of 96 orifice plates pipetting work, simple structure, and the operation of being convenient for has improved the efficiency of branch cup system greatly.
Example two
The embodiment provides a cup separating system, which comprises a transfer mechanism and the multi-channel adjustable-interval pipetting device 100 as described in the first embodiment, wherein the transfer mechanism is used for lifting and transferring the multi-channel adjustable-interval pipetting device 100 so as to assist the multi-channel adjustable-interval pipetting device 100 to complete cup separating operation. The transfer mechanism can be a mechanical arm, and can also be an existing two-dimensional or three-dimensional adjusting platform and the like.
The cup separating system provided by the embodiment can realize quick adjustment of the space of multiple rows and large batches of liquid transferring guns by arranging the multi-channel space adjustable liquid transferring device 100, and greatly improves the efficiency of the cup separating system.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. A multichannel adjustable-spacing pipetting device is characterized by comprising:
the adjusting mechanism comprises an elastic body and an air charging and discharging device, a plurality of rigid structures distributed in a matrix are embedded in the elastic body, and the geometric center of the outer contour of the matrix is superposed with the geometric center of the outer contour of the elastic body; the inflation and deflation device is connected with the elastic body and can inflate or deflate the elastic body to realize the equal proportional amplification or reduction of the space between any two adjacent rows of the rigid structures and the space between any two adjacent columns of the rigid structures in the matrix;
the liquid suction mechanism comprises a plurality of liquid transferring guns and a negative pressure liquid suction device, the liquid transferring guns are arranged on the rigid structure, and the liquid transferring guns are arranged in one-to-one correspondence with the rigid structure; and any one of the liquid-transferring guns is provided with a gas transmission channel, and the gas transmission channel is connected with the negative pressure liquid-absorbing device.
2. A multiple channel adjustable spacing pipetting device as recited in claim 1 wherein the elastomer is a rectangular elastomeric balloon.
3. The multi-channel adjustable-spacing pipetting device of claim 2, wherein the rigid structure is a rigid pipe, any one of the rigid pipe is arranged to penetrate through the top surface and the bottom surface of the rectangular elastic air bag, the outer wall of any one of the rigid pipe is connected with the rectangular elastic air bag in a sealing manner, and the bottom of the inner wall of any one of the rigid pipe is provided with a radial convex ring to fix the pipetting gun.
4. A multi-channel pipetting device with adjustable spacing as recited in claim 3, wherein any one side of the rectangular elastic air bag is provided with a telescopic organ structure surface, and any one of the organ structure surfaces can be telescopic along the direction perpendicular to the rigid pipe.
5. A multi-channel adjustable-pitch pipetting device according to any one of claims 2 to 4, further comprising a guide mechanism, the guide mechanism comprising:
the supporting plate is arranged above the rectangular elastic air bag, two pairs of sliding rails are arranged on the supporting plate, two sliding rails in each pair of sliding rails are positioned on the same straight line, and the projections of the two pairs of sliding rails on the rectangular elastic air bag are respectively superposed with two diagonal lines of the rectangular elastic air bag;
the sliding blocks are in sliding fit with the sliding rails, and each sliding rail is provided with one sliding block in a sliding mode;
the top end of the connecting rod is connected with the sliding block, and the bottom end of the connecting rod is connected with the corner of the rectangular elastic air bag.
6. The multi-channel adjustable-spacing pipetting device as recited in claim 5, wherein the rectangular elastic air bag is provided with connecting lugs at four corners, and the connecting lugs are connected with the bottom ends of the connecting rods.
7. The multi-channel adjustable-pitch pipetting device as recited in claim 6, wherein the connecting rod is a stud bolt, and two ends of the stud bolt are respectively arranged through the sliding block and the connecting lug and are in locking fit with a nut.
8. The multi-channel liquid transfer device with the adjustable space as claimed in any one of claims 1 to 4, wherein the inflation and deflation device comprises an air pipe and an air source, one end of the air pipe is connected with the elastic body, and the other end of the air pipe is connected with the air source.
9. The multi-channel adjustable-spacing pipetting device as recited in any one of claims 1 to 4, wherein the negative pressure pipetting device is a negative pressure gas source, and each gas transmission channel is connected with the negative pressure gas source.
10. A dispensing cup system, comprising a transfer mechanism for lifting and transferring the multi-channel adjustable-pitch pipetting device and the multi-channel adjustable-pitch pipetting device as recited in any one of claims 1 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210798159.7A CN115106142B (en) | 2022-07-06 | 2022-07-06 | Multichannel interval adjustable pipetting device and cup separating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210798159.7A CN115106142B (en) | 2022-07-06 | 2022-07-06 | Multichannel interval adjustable pipetting device and cup separating system |
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| CN115106142A true CN115106142A (en) | 2022-09-27 |
| CN115106142B CN115106142B (en) | 2024-03-29 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050214173A1 (en) * | 2004-01-25 | 2005-09-29 | Fluidigm Corporation | Integrated chip carriers with thermocycler interfaces and methods of using the same |
| CN208975834U (en) * | 2018-10-19 | 2019-06-14 | 南京林业大学 | A kind of liquid-transfering gun and liquid-transfering device |
| CN112169857A (en) * | 2020-10-19 | 2021-01-05 | 西南医科大学 | Rapid arranging and storing box for pipette heads of liquid-transfering gun and using method |
| CN215541001U (en) * | 2021-07-13 | 2022-01-18 | 广东食品药品职业学院 | Interval adjustable double-end liquid-transfering gun |
| CN114367328A (en) * | 2022-02-16 | 2022-04-19 | 安徽医学高等专科学校 | Liquid taking device for biochemical experiments |
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2022
- 2022-07-06 CN CN202210798159.7A patent/CN115106142B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050214173A1 (en) * | 2004-01-25 | 2005-09-29 | Fluidigm Corporation | Integrated chip carriers with thermocycler interfaces and methods of using the same |
| CN208975834U (en) * | 2018-10-19 | 2019-06-14 | 南京林业大学 | A kind of liquid-transfering gun and liquid-transfering device |
| CN112169857A (en) * | 2020-10-19 | 2021-01-05 | 西南医科大学 | Rapid arranging and storing box for pipette heads of liquid-transfering gun and using method |
| CN215541001U (en) * | 2021-07-13 | 2022-01-18 | 广东食品药品职业学院 | Interval adjustable double-end liquid-transfering gun |
| CN114367328A (en) * | 2022-02-16 | 2022-04-19 | 安徽医学高等专科学校 | Liquid taking device for biochemical experiments |
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| CN115106142B (en) | 2024-03-29 |
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