CN115093490A - Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity and preparation method and application thereof - Google Patents
Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity and preparation method and application thereof Download PDFInfo
- Publication number
- CN115093490A CN115093490A CN202210749071.6A CN202210749071A CN115093490A CN 115093490 A CN115093490 A CN 115093490A CN 202210749071 A CN202210749071 A CN 202210749071A CN 115093490 A CN115093490 A CN 115093490A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- asparagus
- low
- molecular
- molecular weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000004676 glycans Chemical class 0.000 title claims abstract description 154
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 154
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 154
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000206611 Gracilariopsis lemaneiformis Species 0.000 title claims description 18
- 241000234427 Asparagus Species 0.000 claims abstract description 100
- 235000005340 Asparagus officinalis Nutrition 0.000 claims abstract description 100
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 51
- 230000015556 catabolic process Effects 0.000 claims abstract description 33
- 238000006731 degradation reaction Methods 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 26
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 25
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 20
- 238000001556 precipitation Methods 0.000 claims abstract description 16
- 238000004108 freeze drying Methods 0.000 claims abstract description 15
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 239000002244 precipitate Substances 0.000 claims abstract description 12
- 238000000502 dialysis Methods 0.000 claims abstract description 11
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 10
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 10
- 239000011718 vitamin C Substances 0.000 claims abstract description 10
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 6
- 235000006708 antioxidants Nutrition 0.000 claims abstract description 6
- 238000000605 extraction Methods 0.000 claims abstract description 5
- 239000000047 product Substances 0.000 claims abstract description 4
- 230000001376 precipitating effect Effects 0.000 claims abstract description 3
- 235000019441 ethanol Nutrition 0.000 claims description 22
- 239000000843 powder Substances 0.000 claims description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 13
- 239000008103 glucose Substances 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 230000000593 degrading effect Effects 0.000 claims description 10
- 238000010828 elution Methods 0.000 claims description 10
- 238000002390 rotary evaporation Methods 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 7
- 229930182830 galactose Natural products 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 7
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 30
- 230000000694 effects Effects 0.000 description 21
- 241000703939 Gracilariopsis longissima Species 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 10
- 230000004792 oxidative damage Effects 0.000 description 8
- 239000002245 particle Substances 0.000 description 7
- 238000009826 distribution Methods 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 150000002772 monosaccharides Chemical class 0.000 description 6
- GHMLBKRAJCXXBS-UHFFFAOYSA-N resorcinol Chemical compound OC1=CC=CC(O)=C1 GHMLBKRAJCXXBS-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- IBZYPBGPOGJMBF-UHFFFAOYSA-N 3,6 anhydrogalactose Natural products CCC=CCC1C(CC(=O)NC(C(C)CC)C(O)=O)CCC1=O IBZYPBGPOGJMBF-UHFFFAOYSA-N 0.000 description 4
- WZYRMLAWNVOIEX-BGPJRJDNSA-N 3,6-anhydro-D-galactose Chemical compound O=C[C@H](O)[C@H]1OC[C@@H](O)[C@@H]1O WZYRMLAWNVOIEX-BGPJRJDNSA-N 0.000 description 4
- DCQFFOLNJVGHLW-UHFFFAOYSA-N 4'-Me ether-Punctatin+ Natural products O1C(O)C(O)C2OCC1C2O DCQFFOLNJVGHLW-UHFFFAOYSA-N 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- WZYRMLAWNVOIEX-UHFFFAOYSA-N cinnamtannin B-2 Natural products O=CC(O)C1OCC(O)C1O WZYRMLAWNVOIEX-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000003642 reactive oxygen metabolite Substances 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000049 pigment Substances 0.000 description 3
- 108010010803 Gelatin Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 229910001626 barium chloride Inorganic materials 0.000 description 2
- 238000004737 colorimetric analysis Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000005457 ice water Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
- 125000002777 acetyl group Chemical class [H]C([H])([H])C(*)=O 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000000089 atomic force micrograph Methods 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000004192 high performance gel permeation chromatography Methods 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- CTYRPMDGLDAWRQ-UHFFFAOYSA-N phenyl hydrogen sulfate Chemical compound OS(=O)(=O)OC1=CC=CC=C1 CTYRPMDGLDAWRQ-UHFFFAOYSA-N 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
- A61K36/04—Rhodophycota or rhodophyta (red algae), e.g. Porphyra
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Sustainable Development (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Materials Engineering (AREA)
- Medical Informatics (AREA)
- Polymers & Plastics (AREA)
- Alternative & Traditional Medicine (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity and a preparation method and application thereof, belonging to the technical field of extraction of algal polysaccharide. The method comprises the following steps: heating and extracting the processed asparagus, centrifuging and concentrating the extracting solution, precipitating with ethanol, dissolving the precipitate again, dialyzing and freeze-drying to obtain asparagus polysaccharide; adding vitamin C and hydrogen peroxide, performing microwave-assisted degradation, and then performing concentration, alcohol precipitation, redissolution, dialysis and freeze drying to obtain the low-molecular-weight crude polysaccharide of the asparagus; purifying to obtain the low-purity molecular weight polysaccharide of the asparagus. The low molecular weight polysaccharide extracted from asparagus of the invention has simple structure, low molecular weight and low viscosity, and is beneficial to development and utilization. Experiments prove that the asparagus low-molecular-weight polysaccharide prepared by the invention has high purity compared with the undegraded common polysaccharide, obviously improves the antioxidant activity, and provides a theoretical basis for development, popularization and application of antioxidant products.
Description
Technical Field
The invention relates to the technical field of extraction of algal polysaccharides, in particular to asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity and a preparation method and application thereof.
Background
The asparagus is an edible economic red alga, the main production places in China are Fujian (75.5%), Guangdong (12.3%) and Shandong (12.3%), and the asparagus is a traditional marine alga plant used as both medicine and food in China. The asparagus can be directly eaten, can be processed into food additives, humectants, gels, medicaments and the like, is widely applied to the industries of food, cosmetics, chemical industry, medicines and the like, and creates great economic value. The main active substance of the asparagus is polysaccharide, and the asparagus has the physiological functions of resisting oxidation, resisting cancer, reducing blood pressure, enhancing immunity and the like. However, the molecular weight of the polysaccharide can significantly affect the biological activity, and if the molecular weight is too large (>1000kDa), the viscosity of the polysaccharide is too high, which is not beneficial to exerting the biological activity, and the development and utilization rate of the polysaccharide is low; while too small a molecular weight (<5kDa), polysaccharides tend to lose biological activity. The asparagus polysaccharide extracted by the prior art often has the problems of large molecular weight, high viscosity and difficult development and utilization, so the invention aims to provide the asparagus polysaccharide with biological activity and high purity and low molecular weight, and provides a theoretical basis for the development and utilization of the asparagus polysaccharide.
Disclosure of Invention
The invention aims to provide asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity, and a preparation method and application thereof, so as to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a preparation method of asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity, which comprises the following steps:
(1) extracting asparagus polysaccharide: cleaning, drying, pulverizing, removing impurities with ethanol, heating, extracting, centrifuging, concentrating, precipitating with ethanol, redissolving the precipitate, dialyzing, and lyophilizing to obtain thallus Gracilariae polysaccharide;
(2) degrading asparagus polysaccharide: adding vitamin C and hydrogen peroxide into the asparagus polysaccharide solution, stirring uniformly, carrying out microwave heating assisted degradation, then concentrating, carrying out alcohol precipitation, dissolving the precipitate again, and then dialyzing and freeze-drying to obtain the asparagus low-molecular-weight crude polysaccharide;
(3) purifying the asparagus low molecular weight polysaccharide: and (3) adding the solution of the low-molecular-weight crude polysaccharide of the asparagus in the step (2) into a glucose gel column for separation and purification to obtain the high-purity low-molecular-weight polysaccharide of the asparagus.
Further, in the step (1), the specific steps of extracting the asparagus polysaccharide comprise: cleaning, drying and crushing asparagus, removing impurities by using 95% ethanol, drying to obtain asparagus powder, and adding a raw material-liquid ratio of 1 g: heating and extracting 40-50mL of water at 80-100 ℃ for 2-4h, centrifuging to obtain supernatant, performing rotary evaporation and concentration at 40-60 ℃, adding 4 times of volume of absolute ethyl alcohol for alcohol precipitation, performing dialysis treatment for 48-72h after precipitation and redissolution, and performing freeze drying for 48-72h to obtain the asparagus polysaccharide.
Further, the heating extraction also comprises ultrasonic auxiliary extraction for 10-40 min; the temperature of the alcohol precipitation is 4 ℃, and the time is 12 h.
Further, in the step (2), the concentration of the asparagus polysaccharide solution is 2-8mg/mL, and the addition amount of the vitamin C and the hydrogen peroxide is 1: 1, in a final concentration of 5-25 mM.
Further, in the step (2), the microwave heating power is 500-900W, the temperature is 40-56 ℃, and the degradation time is 0.1-1 h; the concentration temperature is 40-60 ℃, and the dialysis and freeze drying time is 48-72 h.
Further, in the step (3), the solution concentration of the low molecular weight crude polysaccharide of the asparagus is 2-8mg/mL, the crude polysaccharide is added into a glucose gel G-75 column, elution is carried out by using 0-1M NaCl solution, elution components are collected, detection is carried out by adopting a phenol-sulfuric acid method, and polysaccharide components are collected, so as to obtain the low molecular weight polysaccharide of the asparagus.
The invention also provides the asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity, which is obtained by the preparation method.
Further, it has a molecular weight of 16kDa and consists of galactose and glucose.
The invention also provides application of the asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity in preparation of antioxidant products.
The invention discloses the following technical effects:
the low molecular weight polysaccharide extracted from asparagus of the invention has simple structure, low molecular weight, low viscosity and high purity, and is beneficial to development and utilization. Meanwhile, the safe and nontoxic asparagus polysaccharide is obtained by a water extraction and alcohol precipitation method, and then the asparagus high-purity low-molecular-weight polysaccharide is prepared by degrading with free radicals. In addition, tests prove that the asparagus low-molecular-weight polysaccharide prepared by the invention has high purity compared with the undegraded common polysaccharide, obviously improves the antioxidant activity, and provides a theoretical basis for development, popularization and application of antioxidant products.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a graph of the molecular weight distribution of Gracilaria lemaneiformis polysaccharide before and after degradation;
FIG. 2 is a graph of the elution profile of the purification of Gracilaria lemaneiformis low molecular weight polysaccharides;
FIG. 3 is the change in relative viscosity before and after degradation of Gracilaria lemaneiformis polysaccharide;
FIG. 4 shows XRD analysis results before and after degradation of Gracilaria lemaneiformis polysaccharide;
FIG. 5 is a scanning electron microscope image of Gracilaria lemaneiformis polysaccharide before and after degradation;
FIG. 6 is an atomic force microscope image of Gracilaria lemaneiformis polysaccharide before and after degradation; a: GLP; b: GLP-HV; c: GLP-H; d: GLP-V;
FIG. 7 shows the protective effect of Gracilaria verrucosa polysaccharide on oxidative damage of HepG2 cells before and after degradation;
FIG. 8 is a graph of the effect of Gracilaria verrucosa polysaccharide before and after degradation on oxidative damage to HepG2 cell viability;
FIG. 9 is a graph of the effect of Gracilaria verrucosa polysaccharide on the total antioxidant capacity of HepG2 cells before and after degradation;
FIG. 10 is a graph of the effect of Gracilaria verrucosa polysaccharide before and after degradation on CAT activity of HepG2 cells;
FIG. 11 is a graph of the effect of Gracilaria verrucosa polysaccharide before and after degradation on GSH-PX activity in HepG2 cells;
FIG. 12 is a graph of the effect of Gracilaria verrucosa polysaccharide on SOD activity of HepG2 cells before and after degradation;
FIG. 13 is a graph of the effect of Gracilaria verrucosa polysaccharide before and after degradation on the MDA activity of HepG2 cells;
FIG. 14 shows the interaction of Gracilaria verrucosa polysaccharide before and after degradation with HepG2 cell Ca 2+ The effect of intensity;
FIG. 15 is a graph of the effect of Gracilaria verrucosa polysaccharide degradation on ROS content in HepG2 cells before and after degradation.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in the present disclosure, it is understood that each intervening value, to the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. It is intended that the specification and examples be considered as exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
Example 1
The preparation method of the asparagus high-purity low-molecular-weight polysaccharide comprises the following steps:
(1) pretreatment of asparagus: cleaning, drying and crushing the asparagus, removing pigment and alcohol-soluble impurities by using 95% ethanol, and drying to obtain uniform asparagus powder.
(2) Extracting asparagus polysaccharide: taking the asparagus powder in the step (1), adding pure water, wherein the material-liquid ratio is 1 g: 45mL of the polysaccharide powder, performing ultrasonic-assisted extraction for 30min, heating and extracting at 90 ℃ for 4h, centrifuging to obtain a supernatant, performing rotary evaporation and concentration at 60 ℃, slowly adding 4 times of volume of absolute ethyl alcohol while stirring, placing in a refrigerator at 4 ℃ for standing and alcohol precipitation for 12h, centrifuging to obtain a precipitate, adding pure water for redissolution, performing dialysis treatment for 72h, and finally performing freeze drying for 72h to obtain asparagus polysaccharide powder (GLP).
(3) Degrading asparagus polysaccharide: weighing 18.7mM vitamin C, dissolving in hydrogen peroxide with the same dose, adding into 5mg/mL asparagus polysaccharide solution, stirring uniformly, degrading by microwave (600W, 56 ℃) for 0.5h, carrying out rotary evaporation concentration at 50 ℃, slowly adding 4 times of volume of absolute ethyl alcohol, stirring while adding, placing in a refrigerator at 4 ℃, standing for alcohol precipitation for 12h, centrifuging to obtain precipitate, adding pure water for redissolution, carrying out dialysis treatment for 48h, and finally carrying out freeze drying for 72h to obtain the asparagus low-molecular-weight polysaccharide powder.
(4) Purifying the asparagus low molecular weight polysaccharide: preparing the low molecular weight polysaccharide powder in the step (3) into a 5mg/mL solution, adding the solution into a pretreated glucose gel G-75 column, wherein the sample loading volume is 2% of the column volume, eluting the solution by respectively adopting 0, 0.2, 0.4, 0.6, 0.8 and 1M NaCl solution at the elution flow rate of 0.5mL/min, collecting eluates in different time periods, collecting 50 pipes for each concentration, measuring the eluates by adopting a phenol-sulfuric acid method, and collecting polysaccharide components to obtain the asparagus low molecular weight polysaccharide (GLP-HV).
Example 2
The preparation method of the asparagus high-purity low-molecular-weight polysaccharide comprises the following steps:
(1) pretreatment of asparagus: cleaning, drying and crushing the asparagus, removing pigments and alcohol-soluble impurities by using 95% ethanol, and drying to obtain uniform asparagus powder.
(2) Extracting asparagus polysaccharide: taking the asparagus powder in the step (1), adding pure water, wherein the material-liquid ratio is 1 g: 40mL of the polysaccharide powder is subjected to ultrasonic-assisted extraction for 10min, heating and extracting at 100 ℃ for 3h, centrifuging to obtain a supernatant, performing rotary evaporation and concentration at 50 ℃, slowly adding 4 times of volume of absolute ethyl alcohol while stirring, placing in a refrigerator at 4 ℃ for standing and alcohol precipitation for 12h, centrifuging to obtain a precipitate, adding pure water for redissolution, performing dialysis treatment for 65h, and finally performing freeze drying for 65h to obtain the asparagus polysaccharide powder.
(3) Degrading asparagus polysaccharide: weighing 25mM vitamin C, dissolving in hydrogen peroxide with the same dose, adding into 8mg/mL asparagus polysaccharide solution, stirring uniformly, degrading by microwave (500W, 40 ℃) for 0.1h, carrying out rotary evaporation concentration at 60 ℃, slowly adding 4 times of volume of absolute ethyl alcohol, stirring while adding, placing in a refrigerator at 4 ℃, standing for alcohol precipitation for 12h, centrifuging to obtain precipitate, adding pure water for redissolution, carrying out dialysis treatment for 72h, and finally carrying out freeze drying for 48h to obtain the asparagus low-molecular-weight polysaccharide powder.
(4) Purifying the asparagus low molecular weight polysaccharide: preparing the low molecular weight polysaccharide powder in the step (3) into a solution of 2mg/mL, adding the solution into a pretreated glucose gel G-75 column, wherein the sample loading volume is 5% of the column volume, eluting the solution by respectively adopting 0, 0.2, 0.4, 0.6, 0.8 and 1M NaCl solution, the elution flow rate is 0.4mL/min, collecting the eluates in different time periods, collecting 30 pipes for each concentration, measuring the eluates by adopting a phenol-sulfuric acid method, and collecting polysaccharide components to obtain the asparagus low molecular weight polysaccharide (GLP-HV).
Example 3
The preparation method of the asparagus high-purity low-molecular-weight polysaccharide comprises the following steps:
(1) pretreatment of asparagus: cleaning, drying and crushing the asparagus, removing pigments and alcohol-soluble impurities by using 95% ethanol, and drying to obtain uniform asparagus powder.
(2) Extracting asparagus polysaccharide: taking the asparagus powder in the step (1), adding pure water, wherein the material-liquid ratio is 1 g: 50mL, extracting for 40min under the assistance of ultrasonic waves, heating and extracting at 80 ℃ for 2h, centrifuging to obtain a supernatant, performing rotary evaporation and concentration at 40 ℃, slowly adding 4 times of volume of absolute ethyl alcohol while stirring, placing in a refrigerator at 4 ℃, standing for alcohol precipitation for 12h, centrifuging to obtain a precipitate, adding pure water for redissolution, performing dialysis treatment for 48h, and finally performing freeze drying for 48h to obtain the asparagus polysaccharide powder.
(3) Degrading asparagus polysaccharide: weighing 5mM vitamin C, dissolving in hydrogen peroxide with the same dose, adding into 2mg/mL asparagus polysaccharide solution, stirring uniformly, placing in microwave (900W, 50 ℃) for degradation for 1h, carrying out rotary evaporation concentration at 40 ℃, slowly adding anhydrous ethanol with 4 times of volume, stirring while adding, placing in a refrigerator at 4 ℃ for standing and alcohol precipitation for 12h, centrifuging to obtain precipitate, adding pure water for redissolution, carrying out dialysis treatment for 54h, and finally carrying out freeze drying for 55h to obtain the asparagus low-molecular-weight polysaccharide powder.
(4) Purifying the asparagus low molecular weight polysaccharide: preparing the low molecular weight polysaccharide powder in the step (3) into 8mg/mL solution, adding the solution into a glucose gel G-75 column which is processed in advance, wherein the sample loading volume is 1% of the column volume, eluting the solution by respectively adopting 0, 0.2, 0.4, 0.6, 0.8 and 1M NaCl solution at the elution flow rate of 1.0mL/min, collecting the eluents in different time periods, collecting 80 pipes for each concentration, measuring the eluates by adopting a phenol-sulfuric acid method, and collecting polysaccharide components to obtain the asparagus low molecular weight polysaccharide (GLP-HV).
Comparative example 1
The difference from example 1 is that hydrogen peroxide is omitted in step (3); finally obtaining the asparagus low molecular weight polysaccharide (GLP-H).
Comparative example 2
The difference from example 1 is that vitamin C is omitted in step (3); finally obtaining the asparagus low molecular weight polysaccharide (GLP-V).
Effect example 1
1. Determination of chemical indexes of polysaccharide:
GLP-HV, GLP-H and GLP-V prepared by the method are taken, the total sugar content is measured by adopting a phenol sulfate method, the 3, 6-anhydrogalactose is measured by adopting a resorcinol colorimetric method, the sulfate group content is measured by adopting a gelatin-barium chloride turbidimetric method, and the carbonyl content is measured by adopting a carbonyl kit. The detailed operation steps are as follows:
determination of total sugar content by phenol-sulfuric acid method: taking 1mL of sample, adding 1mL of 5% phenol, mixing uniformly, slowly adding 5mL of concentrated sulfuric acid, mixing uniformly, standing at normal temperature for 30min, cooling at room temperature, and measuring the absorbance value of each tube at 490 nm. And (4) taking galactose as a standard substance, and drawing a standard curve so as to calculate the total sugar content.
Determination of 3, 6-anhydrogalactose by Resorcinol colorimetry: preparing a resorcinol-acetal working solution: respectively taking 9mL of resorcinol solution, 1mL of 0.04% acetal solution and 100mL of concentrated hydrochloric acid, and uniformly mixing to prepare the compound for use. Taking 0.5mL of sample (1mg/mL), placing in an ice-water bath, respectively adding 5mL of resorcinol-acetal working solution, shaking uniformly, reacting at 80 ℃ for 15min, and measuring absorbance at 554nm after 2min of ice-water bath. And drawing a standard curve by taking the fructose as a standard substance so as to calculate the content of the 3, 6-anhydrogalactose.
Measuring the sulfate group content by a gelatin-barium chloride turbidimetry method: weighing 0.01g of sample, adding 1M HCL solution, diluting to 10mL with constant volume, hydrolyzing at 100 ℃ for 6h, taking 0.2mL of hydrolyzed sample, taking HCl solution as blank, and adding 3.8mL of 3% trichloroacetic acid and 1mL of BaCl into each tube respectively 2 Gelatin solution, left at room temperature for 15min, absorbance at 360nm, denoted A 1 (ii) a 1mL of gelatin solution was used instead of BaCl 2 Measuring absorbance by the same method and recording as A 2 (ii) a Absorbance difference A 1 －A 2 The required value is obtained, potassium sulfate is used as a standard substance, a standard curve is drawn, and the sulfate group content is calculated. The results are shown in table 1:
TABLE 1 chemical index changes before and after degradation of Gracilaria lemaneiformis polysaccharide
The total sugar content of the polysaccharide extracted by the method is more than 97 percent, the polysaccharide is degraded by the method, the contents of 3, 6-anhydrogalactose, sulfate and carbonyl are obviously increased, the active groups of the degraded low molecular weight polysaccharide are increased, and a theoretical basis is provided for the increase of the activity of the polysaccharide.
2. Purification of polysaccharide, determination of molecular weight distribution, monosaccharide composition and viscosity:
the molecular weights of the GLP-HV, the GLP-H and the GLP-V prepared in the way are measured by adopting high performance gel permeation chromatography, the results are shown in table 1, after the polysaccharide is degraded by adopting the method, the molecular weight is reduced from 1478kDa to 16kDa, the other two comparative examples are respectively 1329kD and 1000kDa, the data are processed to obtain the molecular weight distribution diagram of figure 1, the result shows that the asparagus polysaccharide possibly contains two main components before degradation, after the asparagus polysaccharide is degraded into the low molecular weight polysaccharide, the molecular weight distribution diagram shows one main component, and the comparative examples 1-2 and the comparative examples before degradation have two components.
The asparagus low molecular weight polysaccharide prepared in example 1 is eluted by NaCl solutions with different concentrations to obtain an elution curve chart shown in figure 2, and the result shows that when the eluent is water, the peak appears, which indicates that the low molecular weight polysaccharide prepared by the invention belongs to neutral polysaccharide, only has one main component, corresponds to the molecular weight distribution chart, and reflects that the purity of the low molecular weight polysaccharide obtained by degrading hydrogen peroxide and vitamin C is higher. Combining the total sugar content in table 1, the molecular weight distribution diagram in fig. 1 and the elution graph in fig. 2, it can be seen that the low molecular weight polysaccharide prepared by the present invention has high purity and can be purified without further purification in the subsequent production process.
The monosaccharide composition of the Gracilaria lemaneiformis low molecular weight polysaccharide prepared in example 1 was analyzed by gel permeation chromatography, and the results showed that the monosaccharide components before degradation of Gracilaria lemaneiformis were mainly glucose (34.35%) and galactose (57.37%), and the monosaccharide components after degradation of the low molecular weight polysaccharide were glucose (33.37%) and galactose (59.12%), respectively. The monosaccharide composition of GLP-H prepared in the comparative example was mainly glucose (24.63%) and galactose (66.39%), the monosaccharide composition of GLP-V was mainly glucose (24.43%) and galactose (69.67%), and the viscosity was measured using the ubbelohde viscometer, and the results showed that when the concentration of polysaccharide was 5mg/mL, the relative viscosities before and after degradation of asparagus were 4.96 and 1.08, respectively, and the degradation significantly reduced the viscosity of polysaccharide, and the viscosities of comparative examples GLP-H and GLP-V were 4.67 and 2.74 (see fig. 3).
3. Polysaccharide crystal result analysis: the XRD diffractometer was used to determine the crystal structure of the polysaccharide, and the results are shown in FIG. 4, in which the low molecular weight polysaccharide prepared by the method of the present invention changed in structure, exhibiting a microcrystalline structure, while the comparative examples 1-2 had a structure between GLP and GLP-HV.
4. Polysaccharide particle size and microstructure analysis: and (3) determining the particle size and microstructure of the polysaccharide before and after degradation by adopting a particle size analyzer, a scanning electron microscope and an atomic force microscope. The results are shown in Table 2:
TABLE 2 particle size change before and after degradation of Gracilaria lemaneiformis polysaccharide
| Particle size | D 10 (μm) | D 50 (μm) | D 90 (μm) |
| GLP | 99.90±6.67A | 258.05±18.82* | 569.91±35.04α |
| GLP-HV | 25.70±0.39C | 85.14±1.42*** | 224.74±7.93γ |
| GLP-H | 55.45±1.98B | 118.64±5.41** | 247.55±15.33βγ |
| GLP-V | 57.12±1.78B | 133.53±5.55** | 283.86±13.87β |
After degradation of the polysaccharide by the process of the invention, its average particle size (D) 50 ) Reduced from 258.05 μm to 85.14 μm, compared with the prior artExamples are 118.64 μm and 133.53 μm, respectively. The results of the analysis by the scanning electron microscope and the atomic force microscope are shown in FIG. 5 and FIG. 6 (in FIG. 6, A: GLP; B: GLP-HV; C: GLP-H; D: GLP-V; and the like), and after the polysaccharide is degraded by the method of the present invention, the microstructure is obviously changed, the sheet structure of the polysaccharide is destroyed, and the structure is loose and broken. The microstructure change of the comparative example was between GLP and GLP-HV. This is consistent with the results for molecular weight and particle size. The polysaccharide prepared by the invention is proved to have low molecular weight, low viscosity and high purity.
Effect example 2
Influence of Gracilaria verrucosa polysaccharide on protection effect and oxidation resistance of oxidative-damaged human liver cancer cells (HepG2) before and after degradation
Adding the asparagus polysaccharide prepared in the example 1 and the degraded low-molecular-weight polysaccharide into HepG2 cells cultured for 24 hours according to a conventional method to continuously culture the cells for 24 hours, inducing the HepG2 cells for 6 hours by using 100 mu g/mL hydrogen peroxide, and researching the protection effect of the cells on the HepG2 cells induced by oxidative damage by using the hydrogen peroxide, wherein a normal group (NC) is the HepG2 cells cultured according to the conventional method; the model set (Mode) is: HepG2 cells which are not added with asparagus polysaccharide and are subjected to oxidative damage induced by hydrogen oxide; fig. 7 and 8 show that before and after the degradation of the asparagus polysaccharide, the asparagus polysaccharide has the protection effect on the HepG2 cells against oxidative damage, the asparagus polysaccharide can remarkably repair the shape and the activity of the oxidative damage cells, and the polysaccharide degraded by the method has more obvious effect. Collecting cell precipitate with oxidative damage, completely cracking the cell, and determining antioxidant activity of the cell with T-AOC, SOD, MDA, GSH-PX, CAT kit, etc. to further verify antioxidant effect of thallus Gracilariae low molecular weight polysaccharide. As a result, as shown in FIGS. 9 to 13, the degraded Gracilaria lemaneiformis low molecular weight polysaccharides had more significant antioxidant activity (note: in FIGS. 7 to 13, "#" indicates a significant difference from the normal group (NC), and "#" indicates a significant difference from the model group (Mode) (p < 0.05)). Ca of asparagus polysaccharide to cells is observed by adopting fluorescent inverted microscope 2+ The strength and ROS (reactive oxygen species) content, and the results in fig. 14 and fig. 15 show that the method for degrading asparagus polysaccharide can obviously reduce Ca of oxidative damage cells 2+ Strength and ROS content, increased finenessAntioxidant capacity of the cells. Whereas the antioxidant capacity of the comparative examples on cells is between GLP and GLP-HV.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (9)
1. A preparation method of asparagus high-purity low-molecular-weight polysaccharide with antioxidant activity is characterized by comprising the following steps:
(1) extracting asparagus polysaccharide: cleaning, drying, pulverizing and removing impurities with ethanol, heating and extracting, centrifuging the extractive solution, concentrating, precipitating with ethanol, dissolving precipitate again, dialyzing, and freeze drying to obtain thallus Gracilariae polysaccharide;
(2) degrading asparagus polysaccharide: adding vitamin C and hydrogen peroxide into the asparagus polysaccharide solution, stirring uniformly, carrying out microwave heating assisted degradation, then concentrating, carrying out alcohol precipitation, dissolving the precipitate again, and then dialyzing and freeze-drying to obtain the asparagus low-molecular-weight crude polysaccharide;
(3) purifying the asparagus low molecular weight polysaccharide: and (3) adding the solution of the low-molecular-weight crude polysaccharide of the asparagus in the step (2) into a glucose gel column for separation and purification to obtain the high-purity low-molecular-weight polysaccharide of the asparagus.
2. The preparation method according to claim 1, wherein in the step (1), the specific steps of extracting the asparagus polysaccharide comprise: cleaning, drying and crushing asparagus, removing impurities by using 95% ethanol, drying to obtain asparagus powder, and adding a raw material-liquid ratio of 1 g: heating and extracting 40-50mL of water at 80-100 ℃ for 2-4h, centrifuging to obtain supernatant, performing rotary evaporation and concentration at 40-60 ℃, adding 4 times of volume of absolute ethyl alcohol for alcohol precipitation, performing dialysis treatment for 48-72h after precipitation and redissolution, and performing freeze drying for 48-72h to obtain the asparagus polysaccharide.
3. The method of claim 2, wherein the heating extraction further comprises ultrasonic assisted extraction for 10-40 min; the temperature of the alcohol precipitation is 4 ℃, and the time is 12 h.
4. The preparation method according to claim 1, wherein in the step (2), the concentration of the asparagus polysaccharide solution is 2-8mg/mL, and the addition amount of the vitamin C and the hydrogen peroxide is 1: 1, in a final concentration of 5-25 mM.
5. The preparation method according to claim 1, wherein in the step (2), the microwave heating power is 500- & 900W, the heating temperature is 40-56 ℃, and the degradation time is 0.1-1 h; the concentration temperature is 40-60 ℃, and the dialysis and freeze drying time is 48-72 h.
6. The preparation method according to claim 1, wherein in the step (3), the solution concentration of the low molecular weight crude polysaccharide of Gracilaria lemaneiformis is 2-8mg/mL, the solution is added into a glucose gel G-75 column, elution is carried out by using 0-1M NaCl solution, elution components are collected, detection is carried out by using a phenol-sulfuric acid method, and polysaccharide components are collected, so as to obtain the low molecular weight polysaccharide of Gracilaria lemaneiformis.
7. A high purity low molecular weight polysaccharide of Gracilaria lemaneiformis having antioxidant activity obtained by the method according to any one of claims 1 to 6.
8. The Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide having antioxidant activity according to claim 7, wherein the polysaccharide has a molecular weight of 16kDa and consists of galactose and glucose.
9. Use of the Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity as defined in claim 7 or 8 in the preparation of antioxidant products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210749071.6A CN115093490A (en) | 2022-06-29 | 2022-06-29 | Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210749071.6A CN115093490A (en) | 2022-06-29 | 2022-06-29 | Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115093490A true CN115093490A (en) | 2022-09-23 |
Family
ID=83294155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210749071.6A Pending CN115093490A (en) | 2022-06-29 | 2022-06-29 | Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115093490A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115611992A (en) * | 2022-10-21 | 2023-01-17 | 上海海洋大学 | Gracilaria lemaneiformis polysaccharide and preparation method and application thereof |
| CN116120481A (en) * | 2023-01-31 | 2023-05-16 | 广东药科大学 | Method for extracting antioxidant active substances from asparagus |
| CN116375901A (en) * | 2023-04-10 | 2023-07-04 | 中国海洋大学 | Preparation method of asparagus fucoidin, obtained fucoidin and application of fucoidin |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112961260A (en) * | 2021-03-23 | 2021-06-15 | 华南理工大学 | Gracilaria lemaneiformis polysaccharide with remarkable anti-inflammatory activity and preparation method and application thereof |
-
2022
- 2022-06-29 CN CN202210749071.6A patent/CN115093490A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112961260A (en) * | 2021-03-23 | 2021-06-15 | 华南理工大学 | Gracilaria lemaneiformis polysaccharide with remarkable anti-inflammatory activity and preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| XIAOSHAN LONG等: ""Optimized Degradation and Inhibition of α-glucosidase Activity by Gracilaria lemaneiformis Polysaccharide and Its Production In Vitro"", 《MAR. DRUGS》 * |
| 杨文鸽等: ""龙须菜多糖的降解及其降解产物的抗氧化活性"", 《水产学报》 * |
| 梁彬: ""分子量对复合多糖抗肿瘤活性的影响"", 《万方数据知识服务平台》 * |
| 王晓梅等: ""龙须菜多糖的提取纯化及生物活性研究进展"", 《海洋渔业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115611992A (en) * | 2022-10-21 | 2023-01-17 | 上海海洋大学 | Gracilaria lemaneiformis polysaccharide and preparation method and application thereof |
| CN116120481A (en) * | 2023-01-31 | 2023-05-16 | 广东药科大学 | Method for extracting antioxidant active substances from asparagus |
| CN116375901A (en) * | 2023-04-10 | 2023-07-04 | 中国海洋大学 | Preparation method of asparagus fucoidin, obtained fucoidin and application of fucoidin |
| CN116375901B (en) * | 2023-04-10 | 2024-03-22 | 中国海洋大学 | Preparation method of asparagus fucoidin, obtained fucoidin and application of fucoidin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115093490A (en) | Gracilaria lemaneiformis high-purity low-molecular-weight polysaccharide with antioxidant activity and preparation method and application thereof | |
| Meng et al. | Structural characterization and immunomodulating activities of polysaccharides from a newly collected wild Morchella sextelata | |
| CN106117389B (en) | Method for extracting and purifying beta-glucan from highland barley grains | |
| CN109750070A (en) | A kind of functionality mulberry leaf oligosaccharide and its preparation method and application | |
| CN111793141B (en) | Pleurotus citrinopileatus mycelium polysaccharide and preparation method and application thereof | |
| CN111704678A (en) | Oyster mushroom galactomannan glucan and preparation method and application thereof | |
| CN110606900B (en) | Method for separating and purifying fructus Jujubae polysaccharide with antioxidant effect | |
| CN114106212B (en) | Bletilla ochracea polysaccharide as well as preparation method and application thereof | |
| CN112724270B (en) | Low-molecular-weight alfalfa polysaccharide, preparation method thereof and application of low-molecular-weight alfalfa polysaccharide in regulating intestinal flora | |
| CN110218262B (en) | Application of low-sulfated heteroglycan rich in glucuronic acid and derived from brown algae in preparation of medicines for treating type 2 diabetes | |
| CN107236054B (en) | Preparation method and application of low-molecular-weight ascophyllan | |
| CN107987179B (en) | Application of low-sulfated fucan in preparation of immunopotentiator | |
| CN111607010A (en) | Enteromorpha polysaccharide with immunoregulation activity and preparation method thereof | |
| CN116589604A (en) | Preparation method of high-purity ginseng polysaccharide | |
| CN113694152B (en) | Method for obtaining coix seed extracting solution by high-stability enzymolysis method | |
| CN104004109A (en) | Ocean sulfated glycosaminoglycan SE-3 and preparation method thereof | |
| CN112794925B (en) | Amomum villosum polysaccharide and preparation method and application thereof | |
| CN113621085A (en) | Preparation method of dandelion root polysaccharide sulfation modification product and application of dandelion root polysaccharide sulfation modification product | |
| CN106754475A (en) | The preparation of lactic acid bacteria WXT002 and exocellular polysaccharide and function | |
| CN108727511B (en) | Carboxymethylated mulberry polysaccharide and preparation method and application thereof | |
| CN111620965A (en) | Preparation method of flammulina velutipes chitin | |
| AU2021100482A4 (en) | An Pleurotus ostreatus Galactomannan and Its Preparation Method and Application | |
| CN106046187B (en) | With free radical cracking product for improving immunocompetent sunset abelmoschus stem or bark leaf polyose and preparation method thereof | |
| CN115232225B (en) | Prepared rehmannia root homogeneous polysaccharide and preparation method and application thereof | |
| CN112724275B (en) | Preparation and application of oak bark polysaccharide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220923 |