CN115092436A - Reagent strip filling device and method thereof - Google Patents
Reagent strip filling device and method thereof Download PDFInfo
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- CN115092436A CN115092436A CN202210869914.6A CN202210869914A CN115092436A CN 115092436 A CN115092436 A CN 115092436A CN 202210869914 A CN202210869914 A CN 202210869914A CN 115092436 A CN115092436 A CN 115092436A
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- reagent strip
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 175
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000007789 sealing Methods 0.000 claims abstract description 67
- 238000005429 filling process Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims description 115
- 238000004140 cleaning Methods 0.000 claims description 100
- 239000011324 bead Substances 0.000 claims description 50
- 102000004190 Enzymes Human genes 0.000 claims description 43
- 108090000790 Enzymes Proteins 0.000 claims description 43
- 238000002372 labelling Methods 0.000 claims description 43
- 239000000758 substrate Substances 0.000 claims description 39
- 210000003437 trachea Anatomy 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 5
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 2
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 2
- 241001330002 Bambuseae Species 0.000 claims description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 2
- 239000011425 bamboo Substances 0.000 claims description 2
- 230000001360 synchronised effect Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000000087 stabilizing effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B3/00—Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans, or jars
- B65B3/26—Methods or devices for controlling the quantity of the material fed or filled
- B65B3/30—Methods or devices for controlling the quantity of the material fed or filled by volumetric measurement
- B65B3/305—Methods or devices for controlling the quantity of the material fed or filled by volumetric measurement with measuring pockets moving in an endless path
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B3/00—Packaging plastic material, semiliquids, liquids or mixed solids and liquids, in individual containers or receptacles, e.g. bags, sacks, boxes, cartons, cans, or jars
- B65B3/26—Methods or devices for controlling the quantity of the material fed or filled
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B43/00—Forming, feeding, opening or setting-up containers or receptacles in association with packaging
- B65B43/42—Feeding or positioning bags, boxes, or cartons in the distended, opened, or set-up state; Feeding preformed rigid containers, e.g. tins, capsules, glass tubes, glasses, to the packaging position; Locating containers or receptacles at the filling position; Supporting containers or receptacles during the filling operation
- B65B43/54—Means for supporting containers or receptacles during the filling operation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
- B65B57/00—Automatic control, checking, warning, or safety devices
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a reagent strip filling device and a method thereof, which comprises a rack, a rear filling assembly, a front filling assembly, a sealing assembly, reagent strips and a controller, the controller controls the first driving motor, the second driving motor and the third driving motor to move the filling part to be inserted into the reagent strip, before the filling process, the air bag is expanded to maintain the sealing between the clamping plate and the reagent strip, so as to avoid splashing to other adjacent hole sites on the reagent strip, inside sucking the reagent of fluid-discharge tube cardboard department into the fluid-discharge tube, avoid appearing the problem of drippage, fill the quantity that the in-process can control the filling reagent respectively through the extension length of the output shaft of control pneumatic cylinder, reach the effect of quantitative filling, solved current reagent strip filling device and easily appear reagent mixed pollution's problem and synchronous filling and lead to the extravagant problem of reagent at the filling in-process.
Description
Technical Field
The invention relates to the technical field of reagent strip filling, in particular to a reagent strip filling device and a reagent strip filling method.
Background
A reagent strip for chemiluminescence quantitative determination, mainly used disease diagnosis's detection, current reagent strip filling device is at the filling in-process, because there is not drip proof structure, the condition that the first department of filling appears remaining reagent drippage after leading to the filling device filling to accomplish, the condition that splashes to adjacent hole site still can appear in the reagent of filling in-process, easily cause reagent mixed contamination's problem, and simultaneously, current reagent strip filling device needs a plurality of filling heads that work as corresponding with reagent strip hole site to carry out synchronous filling to reagent strip filling in-process, because the required reagent volume of filling of different hole sites is inconsistent, synchronous filling can lead to the reagent volume of different hole site fillings unanimously, lead to reagent extravagant.
Disclosure of Invention
The embodiment of the invention provides a reagent strip filling device and a reagent strip filling method.
In view of the above problems, the technical solution proposed by the present invention is:
a reagent strip filling device, comprising:
a frame;
the rear filling assembly comprises a rear filling device, and the rear filling device is arranged on one side of the inside of the rack;
the front filling assembly is arranged on the other side of the rack and comprises a base, a fixed frame, a lifting plate, a vertical lifting device, a transverse moving device, a longitudinal moving device, a driving block and a filling part;
the fixing frame is arranged at the top of the base, the vertical lifting device is arranged on the base, the vertical lifting device is connected with the lifting plate and used for driving the lifting plate to move up and down, the transverse moving device is arranged on the fixing frame, the longitudinal moving device is arranged on the transverse moving device, the driving block is arranged on the longitudinal moving device, the longitudinal moving device is used for driving the driving block to move longitudinally, and the filling part is connected with the driving block;
the rear filling assembly is communicated with the front filling assembly;
the sealing assemblies are respectively arranged on the rear filling device and the front filling assembly;
the reagent strip assembly comprises a reagent strip and a fixed seat, the reagent strip is arranged on the fixed seat, the fixed seat fixes the reagent strip through a groove which is arranged and corresponds to the size of the reagent strip, the fixed seat is arranged in a clamping groove which is formed in the lifting plate, a sample hole, a cleaning hole, a magnetic bead hole, an enzyme labeling hole, a substrate hole, a TIP head cleaning hole and a photometric hole are respectively arranged on the reagent strip, and the filling part is contacted with the reagent strip;
the controller is arranged on one side of the rack and is in communication connection with the rear filling assembly, the front filling assembly and the sealing assembly respectively.
In order to better realize the technical scheme of the invention, the following technical measures are also adopted.
Furthermore, the rear filling device comprises a hydraulic cylinder, an empty cylinder, a piston, a liquid inlet and outlet cylinder, a liquid inlet pipe, a check valve and a liquid discharge pipe, wherein the hydraulic cylinder is fixed on the rack, one end of the empty cylinder is fixedly connected with the hydraulic cylinder, the piston is arranged in the empty cylinder, an output shaft of the hydraulic cylinder is connected with the piston, one end of the liquid inlet and outlet cylinder is communicated with the other end of the empty cylinder, one end of the liquid inlet pipe is communicated with one side of the liquid inlet and outlet cylinder, one end of the liquid inlet pipe is communicated with a reagent, the check valve is arranged between the liquid inlet and outlet cylinder and the liquid inlet pipe, and one end of the liquid discharge pipe is communicated with the other end of the liquid inlet and outlet cylinder.
Further, vertical lift device includes a driving motor, a first lead screw and a first guide bar, two vertical lift device be transversely set up respectively in the both sides of base, a driving motor set up in the inside of base, a lead screw link up the base with a driving motor's output shaft, a guide bar set up in the top of base is located one side of a driving motor, a lead screw with a guide bar runs through respectively the lifter plate, the lifter plate with a lead screw threaded connection, the lifter plate with a guide bar sliding connection.
Further, two the lateral shifting device be vertically set up respectively in on the mount, the lateral shifting device includes second driving motor, second lead screw, second guide bar and first connecting seat, two first connecting seat is transversely to be set up respectively on the mount, the both ends of second guide bar respectively with first connecting seat is connected, second driving motor through first connecting seat with the mount is connected, the one end of second lead screw with second driving motor's output shaft, the other end of second lead screw link up to be kept away from second driving motor one side first connecting seat, and with first connecting seat rotates to be connected.
Furthermore, the longitudinal moving device comprises a third driving motor, a third screw rod, a third guide rod and a second connecting seat, the two second connecting seats are longitudinally arranged on the two transverse moving devices respectively, the second screw rod and the second guide rod are communicated with the second connecting seats respectively, the second connecting seats are in threaded connection with the second screw rod, the second guide rod is in sliding connection with the second connecting seats, the third driving motor is connected with the second connecting seats, one end of the third screw rod is connected with an output shaft of the third driving motor, the other end of the third screw rod is communicated with the second connecting seats far away from one side of the third driving motor and is rotatably connected with the second connecting seats, and two ends of the third guide rod are connected with the two second connecting seats respectively.
Furthermore, the third screw rod and the third guide rod respectively penetrate through the driving block, the driving block is in threaded connection with the third screw rod, and the third guide rod is in sliding connection with the driving block.
Furthermore, the filling part comprises a fixed plate, a sleeve, a liquid flow limiting device, a clamping plate, a conical sleeve and a connecting rod, the fixed plate is connected with the driving block, two ends of the sleeve are sealed, the sleeve is arranged on the fixed plate, the liquid flow limiting device is arranged in the sleeve, one end of the sleeve is connected with the sealing assembly and the rear filling assembly through a sleeve, the clamping plate is connected with the other end of the sleeve through the connecting rod, an annular groove is formed in the edge of the clamping plate, an air vent is formed in the top of the clamping plate, an arc part is arranged on the surface of the bottom of the clamping plate, and the conical sleeve is arranged in the air vent.
Further, the liquid flow limiting device comprises a shell, a sealing plug, a limiting plate and a spring, wherein a flow guide cavity is formed in the shell, a sealing groove is formed in one side of the inner wall of the flow guide cavity, a limiting groove is formed in the other side of the inner wall of the flow guide cavity, one end of the sealing plug is connected with the inside of the sealing groove in an inserted mode, the other end of the sealing plug passes through the spring and is connected with the surface of the inner wall of the flow guide cavity, the limiting plate is arranged on one side of the sealing plug, the limiting plate is connected with the limiting groove in a sliding mode, and the other end of the liquid discharge pipe is sequentially connected with the sleeve, the shell, the connecting rod and the clamping plate and the outside of the clamping plate in a communicated mode.
Furthermore, seal assembly includes air pump, trachea, pressure sensor, gasbag and solenoid valve, the gasbag set up in the inside of ring channel, the air pump set up in the opposite side of an advance and exhaust liquid section of thick bamboo, the output of air pump with tracheal one end is connected, the tracheal other end runs through in proper order the sleeve pipe the sleeve the connecting rod with the cardboard with the gasbag intercommunication, pressure sensor with the solenoid valve sets up respectively on the trachea, the solenoid valve link up sheathed tube one side with sheathed tube outside intercommunication.
A filling method of a reagent strip filling device comprises the following steps:
s1, positioning the reagent strip, fixing the reagent strip through a groove arranged on a fixed seat, and positioning the fixed seat through a clamping groove arranged on a lifting plate;
s2, adjusting the position of the filling part, wherein the controller respectively controls the first driving motor, the second driving motor and the third driving motor to rotate, so that the lifting plate and the filling part are positioned at initial positions, the initial positions are that the bottom of the lifting plate is contacted with the top of the base, the position of the filling part is positioned at the upper left corner, at the moment, the driving block is contacted with the third driving motor, and the second connecting seat is contacted with the second driving motor;
s3, supplementing a reagent, wherein the sealing plug is completely inserted into the sealing groove under the action of the spring, the sealing plug seals the liquid discharge pipe to prevent outside air from entering the liquid discharge pipe, and the controller controls the hydraulic cylinder to drive the reagent communicated with the other end of the liquid inlet pipe to enter the liquid inlet and discharge cylinder through the one-way valve in the retraction process of the piston;
s4, sealing, wherein the controller drives the filling part to move above the reagent strip by respectively controlling a second driving motor and a third driving motor, and respectively inserts six clamping plates into the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole, the substrate hole and the TIP head cleaning hole at the starting position by controlling a first driving motor, wherein the number of the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole and the TIP head cleaning hole is three, the number of the substrate holes is one, the controller starts an air pump, and the air pump conveys air into the air bag through an air pipe to expand the air bag so as to keep the air bag sealed with the clamping plates, the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole, the substrate hole and the TIP head cleaning hole;
s5, filling a reagent for the first time, wherein the controller controls the hydraulic cylinder to extend out, the piston extrudes the reagent in the liquid discharging cylinder, the reagent is respectively filled in the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole, the substrate hole and the TIP head cleaning hole through a liquid discharging pipe and a liquid flow limiting device, and air in the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole, the substrate hole and the TIP head cleaning hole is discharged through the conical sleeve in the filling process;
s6, the reagent flows back, after the pouring is finished, the controller controls the hydraulic cylinder to drive the piston to retract, the reagent communicated with the other end of the liquid inlet pipe enters the liquid inlet and outlet cylinder through the one-way valve, in the retraction process of the piston, the reagent on one end of the liquid outlet pipe positioned on the clamping plate is sucked into the liquid outlet pipe, meanwhile, the liquid outlet pipe is sealed in the process that the sealing plug is inserted into the sealing groove under the action of the spring to prevent the reagent in the liquid outlet pipe from flowing back to the liquid inlet and outlet cylinder completely, the controller closes the air pump to open the electromagnetic valve to exhaust, then the electromagnet is closed, and the air bag contracts back to the inside of the annular groove;
s7, reagent is filled for the second time, the controller separates the clamping plates from the reagent strip by controlling the first driving motor, the six clamping plates of the filling part are moved to the second sample hole, the second cleaning hole, the second magnetic bead hole, the second enzyme labeling hole and the second TIP head cleaning hole of the reagent strip by respectively controlling the second driving motor and the third driving motor, the controller inserts the six clamping plates into the second sample hole, the second cleaning hole, the second magnetic bead hole, the second enzyme labeling hole and the second TIP head cleaning hole by controlling the first driving motor, the controller starts the air pump, and the air pump conveys air to the inside of the air bag through the air pipe to expand the air bag to keep the sealing of the clamping plates, the sample hole, the magnetic bead hole, the enzyme labeling hole, the substrate hole and the TIP head cleaning hole;
s8, repeating the steps S5-S6;
s9, reagent is filled for the third time, the controller separates the clamping plates from the reagent strips by controlling the first driving motor, the six clamping plates of the filling part are moved to the third sample hole, the third cleaning hole, the third magnetic bead hole, the third enzyme labeling hole and the third TIP head cleaning hole of the reagent strips by respectively controlling the second driving motor and the third driving motor, the controller inserts the six clamping plates into the third sample hole, the third cleaning hole, the third magnetic bead hole, the third enzyme labeling hole and the third TIP head cleaning hole by controlling the first driving motor, the controller starts the air pump, and the air pump conveys air to the inside of the air bag through the air pipe to expand the air bag to keep the sealing of the clamping plates, the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole, the substrate hole and the TIP head cleaning hole;
s10, repeating the steps S5-S6.
Compared with the prior art, the invention has the beneficial effects that: the controller controls the first driving motor, the second driving motor and the third driving motor to move the filling part to be inserted into the reagent strip, before the filling process, the controller starts the air pump, the air pump conveys air into the air bag through the air pipe to enable the air bag to expand to keep the air bag to seal the clamping plate, the sample hole, the cleaning hole, the magnetic bead hole, the enzyme labeling hole, the bottom hole and the TIP head cleaning hole, the reagent is prevented from splashing to other adjacent hole sites on the reagent strip in the filling process, after the filling is finished, the reagent enters the liquid inlet and outlet cylinder through the one-way valve, in the retraction process of the piston, the reagent on one end of the liquid outlet pipe is sucked into the liquid outlet pipe, meanwhile, the sealing plug is inserted into the sealing groove under the action of the spring to seal the liquid outlet pipe, the reagent in the liquid outlet pipe is prevented from completely flowing back to the liquid inlet and outlet cylinder, and the influence of an air section in the liquid outlet pipe on the accuracy of the filling quantity in the next filling process is reduced, simultaneously with the reagent suction fluid-discharge tube inside of fluid-discharge tube cardboard department, avoid appearing the problem of drippage, fill the quantity that the in-process can control the filling reagent respectively through the extension length of the output shaft of control pneumatic cylinder, reach the effect of quantitative filling, solved current reagent strip filling device and easily appear reagent mixed pollution's problem and synchronous filling and lead to the extravagant problem of reagent at the filling in-process.
The foregoing description is only an overview of the technical solutions of the present invention, and the embodiments of the present invention are described below in order to make the technical means of the present invention more clearly understood and to make the above and other objects, features, and advantages of the present invention more clearly understandable.
Drawings
Fig. 1 is a schematic view structure diagram of a reagent strip filling apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a front-loading assembly according to an embodiment of the present disclosure;
FIG. 3 is an enlarged schematic view of the structure at A in FIG. 2;
FIG. 4 is an enlarged view of the structure at B in FIG. 2;
FIG. 5 is an enlarged view of the structure at C in FIG. 2;
FIG. 6 is an enlarged view of the structure of FIG. 2 at D;
FIG. 7 is a schematic structural diagram of a filling section and a reagent strip according to an embodiment of the present invention;
fig. 8 is a schematic view of another perspective structure of the reagent strip filling apparatus according to the embodiment of the present invention;
fig. 9 is a schematic structural diagram of a post-filling device according to an embodiment of the disclosure;
FIG. 10 is an enlarged view of the structure at A in FIG. 9;
fig. 11 is a schematic partial sectional structural view of a post-filling device according to an embodiment of the disclosure;
FIG. 12 is a schematic view, partly in section, of a portion of the filling section according to the embodiment of the disclosure;
FIG. 13 is a partial cross-sectional view of another portion of the filling section according to the embodiment of the present disclosure;
fig. 14 is a communication block diagram of a reagent strip filling apparatus according to an embodiment of the present invention;
fig. 15 is a schematic flow chart of a filling method of a reagent strip filling device according to an embodiment of the present invention.
Reference numerals are as follows: 1. a frame; 2. a rear filling assembly; 21. a post-filling device; 211. a hydraulic cylinder; 212. an empty cartridge; 213. a piston; 214. a liquid inlet and outlet cylinder; 215. a liquid inlet pipe; 216. a one-way valve; 217. a liquid discharge pipe; 3. a sleeve; 4. a seal assembly; 41. an air pump; 42. an air tube; 43. a pressure sensor; 44. an air bag; 45. an electromagnetic valve; 5. a front fill assembly; 51. a base; 52. a fixed mount; 53. a lifting plate; 531. a card slot; 54. a vertical lifting device; 541. a first drive motor; 542. a first lead screw; 543. a first guide bar; 55. a lateral movement device; 551. a second drive motor; 552. a second screw rod; 553. a second guide bar; 554. a first connecting seat; 56. a longitudinal moving device; 561. a third drive motor; 562. a third screw rod; 563. a third guide bar; 564. a second connecting seat; 57. a drive block; 58. a filling section; 581. a fixing plate; 582. a sleeve; 583. a liquid flow restriction device; 5831. a housing; 58311. a flow guide cavity; 58312. a sealing groove; 58313. a limiting groove; 5832. a sealing plug; 5833. a limiting plate; 5834. a spring; 584. clamping a plate; 5841. an annular groove; 5842. a vent hole; 5843. an arc portion; 585. a conical sleeve; 586. a connecting rod; 6. a reagent strip assembly; 61. a reagent strip; 611. a sample well; 612. cleaning the holes; 613. magnetic bead holes; 614. enzyme labeling holes; 615. a substrate hole; 616. cleaning holes by TIP heads; 62. a fixed seat; 621. a groove; 7. and a controller.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-14, a reagent strip filling apparatus comprises a frame 1, a rear filling assembly 2, a front filling assembly 5, a sealing assembly 4 and a controller 7, wherein the rear filling assembly 2 comprises a rear filling device 21, the rear filling device 21 is arranged on one side of the inside of the frame 1, the front filling assembly 5 is arranged on the other side of the frame 1, the front filling assembly 5 comprises a base 51, a fixing frame 52, a lifting plate 53, a vertical lifting device 54, a transverse moving device 55, a longitudinal moving device 56, a driving block 57 and a filling part 58, the fixing frame 52 is arranged on the top of the base 51, the vertical lifting device 54 is arranged on the base 51, the vertical lifting device 54 is connected with the lifting plate 53 and used for driving the lifting plate 53 to move up and down, the transverse moving device 55 is arranged on the fixing frame 52, the longitudinal moving device 56 is arranged on the transverse moving device 55, the driving block 57 is arranged on the longitudinal moving device 56, the longitudinal moving device 56 is used for driving the driving block 57 to move longitudinally, the filling part 58 is connected with the driving block 57, the rear filling component 2 is communicated with the front filling component 5, the sealing components 4 are respectively arranged on the rear filling device 21 and the front filling component 5, the reagent strip component 6 comprises a reagent strip 61 and a fixed seat 62, the reagent strip 61 is arranged on the fixed seat 62, the fixed seat 62 fixes the reagent strip 61 through a groove 621 which is arranged and corresponds to the size of the reagent strip 61, the fixed seat 62 is arranged in a clamping groove 531 which is arranged on the lifting plate 53, a sample hole 611, a cleaning hole 612, a magnetic bead hole 613, an enzyme labeling hole 614, a substrate hole 615, a TIP head cleaning hole 616 and a photometric hole are respectively arranged on the reagent strip 61, the filling part 58 is in contact with the reagent strip 61, the controller 7 is arranged on one side of the rack 1, and the controller 7 is respectively connected with the rear filling component 2, the front filling component 5 and the reagent strip 61, The front fill assembly 5 and the seal assembly 4 are communicatively connected.
The embodiment of the invention is also realized by the following technical scheme.
Referring to fig. 1 and 8-11, in an embodiment of the present invention, the post-filling device 21 includes a hydraulic cylinder 211, an empty cylinder 212, a piston 213, a liquid inlet and outlet cylinder 214, a liquid inlet pipe 215, a check valve 216, and a liquid outlet pipe 217, the hydraulic cylinder 211 is fixed on the frame 1, one end of the empty cylinder 212 is fixedly connected to the hydraulic cylinder 211, the piston 213 is disposed inside the empty cylinder 212, an output shaft of the hydraulic cylinder 211 is connected to the piston 213, one end of the liquid inlet and outlet cylinder 214 is communicated to the other end of the empty cylinder 212, one end of the liquid inlet pipe 215 is communicated to one side of the liquid inlet and outlet cylinder 214, one end of the liquid inlet pipe 215 is communicated with a reagent, the check valve 216 is disposed between the liquid inlet and outlet cylinder 214 and the liquid inlet pipe 215, and one end of the liquid outlet pipe 217 is communicated to the other end of the liquid inlet and outlet cylinder 214.
It should be noted that the check valve 216 is used to prevent the reagent in the liquid inlet and outlet cylinder 214 from flowing back into the liquid inlet pipe 215, the number of the post-filling devices 21 is eight, and the post-filling devices 21 correspond to the sample holes 611, the cleaning holes 612, the magnetic bead holes 613, the enzyme labeling holes 614, the substrate holes 615, and the TIP head cleaning holes 616 arranged on the reagent strip 61, different post-filling devices 21 are respectively used to connect different reagents required by the sample holes 611, the cleaning holes 612, the magnetic bead holes 613, the enzyme labeling holes 614, and the TIP head cleaning holes 616, the number of the sample holes 611, the cleaning holes 612, the magnetic bead holes 613, the enzyme labeling holes 614, and the TIP head cleaning holes 616 is three, the number of the substrate holes 615 is one, for example, the amounts of the reagents filled in three adjacent sample holes 611 are different according to requirements, and the protruding length of the output shaft of the hydraulic cylinder 211 can be controlled to fill the sample holes 611, the cleaning holes 612, the substrate holes 615, and the TIP head cleaning holes 616 respectively, The quantity of the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP head cleaning hole 616 reagent achieves the effect of quantitative filling.
Referring to fig. 1 to 6, in the embodiment of the present invention, the vertical lifting device 54 includes a first driving motor 541, a first lead screw 542 and a first guiding rod 543, the two vertical lifting devices 54 are transversely disposed on two sides of the base 51, respectively, the first driving motor 541 is disposed inside the base 51, the first lead screw 542 penetrates through the base 51 and is connected to an output shaft of the first driving motor 541, the first guiding rod 543 is disposed on a top portion of the base 51 and is located on one side of the first driving motor 541, the first lead screw 542 and the first guiding rod 543 respectively penetrate through the lifting plate 53, the lifting plate 53 is in threaded connection with the first lead screw 542, the lifting plate 53 is in sliding connection with the first guiding rod 543, the two lateral moving devices 55 are longitudinally disposed on the fixing frame 52, respectively, the lateral moving device 55 includes a second driving motor 551, a second lead screw 552, a second guiding rod and a first connecting base 554, two first connecting seats 554 are transversely arranged on the fixing frame 52 respectively, two ends of a second guide rod 553 are connected with the first connecting seats 554 respectively, a second driving motor 551 is connected with the fixing frame 52 through the first connecting seats 554, one end of a second lead screw 552 is connected with an output shaft of the second driving motor 551, the other end of the second lead screw 552 penetrates through the first connecting seat 554 at the side far away from the second driving motor 551 and is rotatably connected with the first connecting seats 554, a longitudinal moving device 56 comprises a third driving motor 561, a third lead screw 562, a third guide rod 563 and a second connecting seat 564, two second connecting seats 564 are longitudinally arranged on the two transverse moving devices 55 respectively, the second lead screw 552 and the second guide rod 553 penetrate through the second connecting seats 564 respectively, the second connecting seat 564 is in threaded connection with the second lead screw 552, the second guide rod 553 is in sliding connection with the second connecting seats, the third driving motor 561 is connected with the second connecting seats 564, one end of the third screw 562 is connected with an output shaft of the third driving motor 561, the other end of the third screw 562 penetrates through the second connecting seat 564 far away from one side of the third driving motor 561 and is rotatably connected with the second connecting seat 564, two ends of the third guide rod 563 are respectively connected with the two second connecting seats 564, the third screw 562 and the third guide rod 563 penetrate through the driving block 57 respectively, the driving block 57 is in threaded connection with the third screw 562, and the third guide rod 563 is in sliding connection with the driving block 57.
It should be noted that, the first driving motor 541 is used for driving the first lead screw 542 to rotate, the lifting plate 53 is driven to drive the reagent strip 61 to approach or leave the filling portion 58, the first guiding rod 543 is used for guiding the lifting plate 53 and stabilizing a lifting process of the lifting plate 53, the second driving motor 551 is used for driving the second lead screw 552 to rotate, the second connecting seat 564 is driven to drive the filling portion 58 to move transversely, the second guiding rod 553 is used for guiding the second connecting seat 564 and stabilizing a transverse moving process of the second connecting seat 564, the third driving motor 561 is used for driving the third lead screw 562 to rotate, the driving block 57 is driven to drive the filling portion 58 to move longitudinally, the third guiding rod 563 is used for guiding the driving block 57 and stabilizing a longitudinal moving process of the driving block 57, the movable filling part 58 can complete the whole filling process of the reagent strips 61 on the fixed seat 62 by controlling the first driving motor 541, the second driving motor 551 and the third driving motor 561.
Referring to fig. 1-3, 7, 12 and 13, in an embodiment of the present invention, the filling unit 58 includes a fixing plate 581, a sleeve 582, a flow restriction device 583, a clamp plate 584, a tapered sleeve 585 and a connecting rod 586, the fixing plate 581 is connected to the driving block 57, both ends of the sleeve 582 are closed, the sleeve 582 is disposed on the fixing plate 581, the flow restriction device 583 is disposed inside the sleeve 582, one end of the sleeve 582 is connected to the sealing assembly 4 and the rear filling assembly 2 through the bushing 3, the clamp plate 584 is connected to the other end of the sleeve 582 through the connecting rod 586, an annular groove 5841 is disposed at an edge of the clamp plate 584, a vent hole 5842 is disposed at a top of the clamp plate 584, a circular arc portion 5843 is disposed on a bottom surface of the clamp plate 584, the tapered sleeve 585 is disposed inside the vent hole 5842, the flow restriction device 583 includes a housing 5831, a sealing plug 5832, a restriction plate 5833 and a spring 5834, a flow guide chamber 58311 is disposed inside the housing 5831, seal groove 58312 has been seted up to the inner wall one side of water conservancy diversion chamber 58311, spacing groove 58313 has been seted up to the inner wall opposite side of water conservancy diversion chamber 58311, the inside of one end grafting and seal groove 58312 of sealing plug 5832, the other end of sealing plug 5832 passes through spring 5834 and is connected with the inner wall surface of water conservancy diversion chamber 58311, limiting plate 5833 sets up in one side of sealing plug 5832, limiting plate 5833 and spacing groove 58313 sliding connection, the other end of fluid-discharge tube 217 link up connecting sleeve 3 in proper order, sleeve 582, casing 5831, connecting rod 586 and cardboard 584 and the outside of cardboard 584 intercommunication.
It should be noted that the number of the sleeve 582, the flow restriction device 583, the card 584, the taper sleeve 585 and the connecting rod 586 is eight, which corresponds to the corresponding rear filling device 21, and is used to fill different reagents into the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614, the substrate hole 615 and the TIP head cleaning hole 616 on the reagent strip 61, the size of the card 584 is respectively matched with the size and shape of the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614, the substrate hole 615 and the TIP head cleaning hole 616 on the reagent strip 61, the size and shape of the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614, the substrate hole 615 and the TIP head cleaning hole 616 on the reagent strip 61 are consistent, during the reagent filling process, the reagent in the liquid discharge tube 217 enters the sealing groove 58312 to push the sealing plug 5832 into the flow guide cavity 58311, during which, the 5832 compresses the spring 5834 under the position restriction of the restriction plate 5833, the number of the limiting plates 5833 is two, the limiting plates are distributed at two sides of the sealing plug 5832, the reagent enters the flow guide cavity 58311 and is poured into the reagent strip 61 from the clamping plate 584, after the pouring is finished, the controller 7 controls the hydraulic cylinder 211 to drive the piston 213 to retract, the reagent communicated with the other end of the liquid inlet pipe 215 enters the liquid inlet and outlet cylinder 214 through the one-way valve 216, in the retracting process of the piston 213, the reagent positioned on one end of the clamping plate 584 of the liquid outlet pipe 217 is sucked into the liquid outlet pipe 217, meanwhile, the sealing plug 5832 is inserted into the sealing groove 58312 under the action of the spring 5834 to seal the liquid outlet pipe 217 so as to prevent the reagent in the liquid outlet pipe 217 from completely flowing back into the liquid outlet cylinder 214, the influence of the air section in the liquid outlet pipe 217 in the next pouring process on the pouring amount accuracy is reduced, meanwhile, the reagent in the position of the clamping plate 584 of the liquid outlet pipe 217 is sucked into the liquid outlet pipe 217 so as to avoid the dripping problem, in the pouring process, the taper sleeve 585 is used for exhausting, blocking splashed reagent droplets in the process of filling the liquid discharge pipe 217, avoiding splashing to other adjacent hole sites on the reagent strip 61 to cause mixed pollution, and the circular arc portion 5843 arranged at the bottom of the clamping plate 584 can avoid the reagent from being attached to the bottom of the clamping plate 584.
Referring to fig. 3-7, in the embodiment of the present invention, the sealing assembly 4 includes an air pump 41, an air tube 42, a pressure sensor 43, an air bag 44 and a solenoid valve 45, the air bag 44 is disposed inside the annular groove 5841, the air pump 41 is disposed on the other side of the liquid inlet and outlet barrel 214, an output end of the air pump 41 is connected to one end of the air tube 42, the other end of the air tube 42 sequentially penetrates through the sleeve 3, the sleeve 582, the connecting rod 586 and the snap plate 584 to communicate with the air bag 44, the pressure sensor 43 and the solenoid valve 45 are respectively disposed on the air tube 42, and the solenoid valve 45 penetrates through one side of the sleeve 3 to communicate with the outside of the sleeve 3.
Specifically, six clamping plates 584 are respectively inserted into the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP head cleaning hole 616 at the beginning, the number of the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614 and the TIP head cleaning hole 616 is three, the number of the substrate holes 615 is one, the controller 7 starts the air pump 41, the air pump 41 supplies air to the air bag 44 through the air pipe 42 to expand the air bag 44 to maintain the sealing between the clamping plates 584 and the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP head cleaning hole 616, and the adjacent other hole sites on the reagent strip 61 are prevented from being splashed during the filling process to cause mixed pollution.
The signal input end of the controller 7 is in communication connection with the signal output end of the pressure sensor 43, and the signal output end of the controller 7 is in communication connection with the signal input ends of the hydraulic cylinder 211, the air pump 41, the electromagnetic valve 45, the first driving motor 541, the second driving motor 551 and the third driving motor 561 respectively.
Referring to fig. 1-15, the invention also provides a filling method of the reagent strip filling device, which comprises the following steps:
s1, positioning the reagent strip, namely fixing the reagent strip 61 through a groove 621 formed in a fixed seat 62, and positioning the fixed seat 62 through a clamping groove 531 formed in a lifting plate 53;
s2, adjusting the position of the filling part, the controller 7 controlling the first driving motor 541, the second driving motor 551 and the third driving motor 561 to rotate respectively, so that the lifting plate 53 and the filling part 58 are at initial positions, the initial position is that the bottom of the lifting plate 53 contacts with the top of the base 51, the position of the filling part 58 is at the upper left corner, at this time, the driving block 57 contacts with the third driving motor 561, and the second connecting seat 564 contacts with the second driving motor 551;
s3, supplementing a reagent, completely inserting the sealing plug 5832 into the sealing groove 58312 under the action of the spring 5834, sealing the liquid discharge pipe 217 by the sealing plug 5832, preventing outside air from entering the inside of the liquid discharge pipe 217, controlling the hydraulic cylinder 211 by the controller 7 to drive the piston 213 to retract, and enabling the reagent communicated with the other end of the liquid inlet pipe 215 to enter the inside of the liquid inlet and discharge cylinder 214 through the check valve 216;
s4, sealing, the controller 7 drives the filling part 58 to move above the reagent strip 61 by controlling the second driving motor 551 and the third driving motor 561 respectively, and inserts six clamping plates 584 into the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614, the substrate hole 615 and the TIP head cleaning hole 616 respectively by controlling the first driving motor 541, the number of the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614 and the TIP head cleaning hole 616 is three, the number of the substrate holes 615 is one, the controller 7 starts the air pump 41, the air pump 41 sends air into the air bag 44 through the air pipe 42 to expand the air bag 44 to keep the sealing between the clamping plates 584 and the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614, the substrate hole 615 and the TIP head cleaning hole 616;
s5, reagent filling is performed for the first time, the controller 7 controls the hydraulic cylinder 211 to extend, the piston 213 presses the reagent in the liquid discharging cylinder 214, the reagent is respectively filled in the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP head cleaning hole 616 through the liquid discharging pipe 217 and the liquid flow limiting device 583, and air in the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP head cleaning hole 616 is discharged through the conical sleeve 585 during filling;
s6, the reagent flows back, after the pouring is finished, the controller 7 controls the hydraulic cylinder 211 to drive the piston 213 to retract, the reagent communicated with the other end of the liquid inlet pipe 215 enters the liquid inlet and outlet cylinder 214 through the one-way valve 216, in the retraction process of the piston 213, the reagent on one end of the liquid outlet pipe 217 on the clamping plate 584 is sucked into the liquid outlet pipe 217, meanwhile, the sealing plug 5832 is inserted into the sealing groove 58312 to seal the liquid outlet pipe 217 so as to prevent the reagent in the liquid outlet pipe 217 from flowing back to the liquid inlet and outlet cylinder 214 completely, the controller 7 closes the air pump 41 to open the electromagnetic valve 45 to exhaust, then closes the electromagnet, and the air bag 44 contracts back to the inside of the annular groove 5841;
s7, reagent filling for the second time, the controller 7 separates the card 584 from the reagent strip by controlling the first driving motor 541, the six clamping plates 584 of the filling part 58 are driven to move above the second sample hole 611, the second cleaning hole 612, the second magnetic bead hole 613, the second enzyme labeling hole 614 and the second TIP cleaning hole 616 of the reagent strip 61 by respectively controlling the second driving motor 551 and the third driving motor 561, the controller 7 inserts the six clamping plates 584 into the second sample hole 611, the second cleaning hole 612, the second magnetic bead hole 613, the second enzyme labeling hole 614 and the second TIP cleaning hole 616 by controlling the first driving motor 541, the controller 7 starts the air pump 41, and the air pump 41 supplies air into the air bag 44 through the air pipe 42 to expand the air bag 44 to keep the sealing between the clamping plates 584 and the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP cleaning hole 616;
s8, repeating the steps S5-S6;
s9, reagent filling for the third time, the controller 7 separates the card 584 from the reagent strip by controlling the first driving motor 541, the six card plates 584 of the filling part 58 are driven to move above the third sample hole 611, the third cleaning hole 612, the third magnetic bead hole 613, the third enzyme labeling hole 614 and the third TIP cleaning hole 616 of the reagent strip 61 by controlling the second driving motor 551 and the third driving motor 561 respectively, the controller 7 inserts the six card plates 584 into the third sample hole 611, the third cleaning hole 612, the third magnetic bead hole 613, the third enzyme labeling hole 614 and the third TIP cleaning hole 616 by controlling the first driving motor 541, the controller 7 starts the air pump 41, and the air pump 41 supplies air into the air bag 44 through the air pipe 42 to expand the air bag 44 to maintain the sealing between the card plates 584 and the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP cleaning hole 616.
It should be noted that, in the filling process, the amount of the filling reagent can be respectively controlled by controlling the extension length of the output shaft of the hydraulic cylinder 211, so as to achieve the quantitative filling effect, and compared with synchronous filling, the amount of the filling reagent in the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the enzyme labeling hole 614, the substrate hole 615 and the TIP head cleaning hole 616 can be quantitatively controlled, and because there is only one substrate hole 615, the hydraulic cylinder 211 of the filling device for filling the substrate hole 615 does not act in the second reagent filling process and the third reagent filling process;
s10, repeating the steps S5-S6.
Specifically, the controller 7 controls the first driving motor 541, the second driving motor 551 and the third driving motor 561 to move the filling part 58 to insert into the reagent strip 61, before the filling process, the controller 7 starts the air pump 41, the air pump 41 pumps air into the air bag 44 through the air tube 42 to inflate the air bag 44 to maintain the sealing between the clamping plate 584 and the sample hole 611, the cleaning hole 612, the magnetic bead hole 613, the elisa hole 614, the substrate hole 615 and the TIP cleaning hole 616, so as to avoid splashing to other adjacent holes on the reagent strip 61 during the filling process, after the filling process, the reagent on one end of the clamping plate 584 of the liquid discharge tube 217 is sucked into the liquid discharge tube 217 during the retraction process of the piston 213, and the sealing plug 5832 is inserted into the sealing groove 58312 under the action of the spring 5834 to close the liquid discharge tube 217 so as to avoid the reagent in the liquid discharge tube 217 from flowing back into the liquid discharge tube 214 completely, reduce the influence of the air section in the next filling in-process fluid-discharge tube 217 to the filling volume accuracy, inhale the fluid-discharge tube 217 with the reagent of fluid-discharge tube 217 cardboard 584 department inside simultaneously, avoid appearing the problem of drippage, the volume of filling reagent can be controlled respectively to the extension length of the output shaft through control pneumatic cylinder 211 to the filling in-process, reach the effect of quantitative filling, the problem of reagent mixed pollution and the synchronous filling lead to the extravagant problem of reagent have easily appeared at the filling in-process to have solved current reagent strip filling device.
It should be noted that the specific model specifications of the hydraulic cylinder 211, the air pump 41, the pressure sensor 43, the electromagnetic valve 45, the first driving motor 541, the second driving motor 551, the third driving motor 561 and the controller 7 need to be determined according to the actual specification of the device, and the specific model selection calculation method adopts the prior art in the field, and therefore, detailed description is omitted.
The power supply and the principle of the hydraulic cylinder 211, the air pump 41, the pressure sensor 43, the solenoid valve 45, the first driving motor 541, the second driving motor 551, the third driving motor 561 and the controller 7 will be apparent to those skilled in the art and will not be described in detail herein.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The utility model provides a reagent strip filling device which characterized in that includes:
a frame (1);
a rear filling assembly (2), wherein the rear filling assembly (2) comprises a rear filling device (21), and the rear filling device (21) is arranged on one side of the interior of the rack (1);
the front filling assembly (5) is arranged on the other side of the rack (1), and the front filling assembly (5) comprises a base (51), a fixing frame (52), a lifting plate (53), a vertical lifting device (54), a transverse moving device (55), a longitudinal moving device (56), a driving block (57) and a filling part (58);
the fixing frame (52) is arranged at the top of the base (51), the vertical lifting device (54) is arranged on the base (51), the vertical lifting device (54) is connected with the lifting plate (53) and used for driving the lifting plate (53) to move up and down, the transverse moving device (55) is arranged on the fixing frame (52), the longitudinal moving device (56) is arranged on the transverse moving device (55), the driving block (57) is arranged on the longitudinal moving device (56), the longitudinal moving device (56) is used for driving the driving block (57) to move longitudinally, and the filling part (58) is connected with the driving block (57);
the rear filling assembly (2) is communicated with the front filling assembly (5);
a sealing assembly (4), the sealing assembly (4) being respectively arranged on the rear filling device (21) and the front filling assembly (5);
the reagent strip assembly (6) comprises a reagent strip (61) and a fixed seat (62), the reagent strip (61) is arranged on the fixed seat (62), the fixed seat (62) fixes the reagent strip (61) through a groove (621) which is arranged and corresponds to the reagent strip (61) in size, the fixed seat (62) is arranged in a clamping groove (531) which is formed in the lifting plate (53), a sample hole (611), a cleaning hole (612), a magnetic bead hole (613), an enzyme labeling hole (614), a substrate hole (615), a TIP head cleaning hole (616) and a photometric hole are respectively formed in the reagent strip (61), and the filling part (58) is in contact with the reagent strip (61);
a controller (7), controller (7) set up in one side of frame (1), controller (7) respectively with rear portion filling subassembly (2), anterior filling subassembly (5) and sealing component (4) communication connection.
2. The reagent strip filling device according to claim 1, wherein: the rear filling device (21) comprises a hydraulic cylinder (211), an empty cylinder (212), a piston (213), a liquid inlet and outlet cylinder (214), a liquid inlet pipe (215), a one-way valve (216) and a liquid outlet pipe (217), the hydraulic cylinder (211) is fixed on the frame (1), one end of the hollow cylinder (212) is fixedly connected with the hydraulic cylinder (211), the piston (213) is arranged in the hollow cylinder (212), the output shaft of the hydraulic cylinder (211) is connected with the piston (213), one end of the liquid inlet and outlet cylinder (214) is communicated with the other end of the empty cylinder (212), one end of the liquid inlet pipe (215) is communicated with one side of the liquid inlet and outlet cylinder (214), one end of the liquid inlet pipe (215) is communicated with a reagent, the one-way valve (216) is arranged between the liquid inlet and outlet cylinder (214) and the liquid inlet pipe (215), one end of the drain pipe (217) is communicated with the other end of the liquid inlet and outlet cylinder (214).
3. The reagent strip filling device according to claim 1, wherein: the vertical lifting device (54) comprises a first driving motor (541), a first screw rod (542) and a first guide rod (543), two vertical lifting devices (54) are transversely arranged on two sides of the base (51) respectively, the first driving motor (541) is arranged inside the base (51), the first screw rod (542) penetrates through the base (51) and an output shaft of the first driving motor (541), the first guide rod (543) is arranged at the top of the base (51) and is located on one side of the first driving motor (541), the first screw rod (542) and the first guide rod (543) penetrate through the lifting plate (53) respectively, the lifting plate (53) is in threaded connection with the first screw rod (542), and the lifting plate (53) is in sliding connection with the first guide rod (543).
4. A reagent strip filling apparatus according to claim 3, wherein: the two transverse moving devices (55) are respectively arranged on the fixed frame (52) in a longitudinal direction, the transverse moving device (55) comprises a second driving motor (551), a second screw rod (552), a second guide rod (553) and first connecting seats (554), the two first connecting seats (554) are respectively transversely arranged on the fixed frame (52), both ends of the second guide bar (553) are respectively connected with the first connection seat (554), the second driving motor (551) is connected with the fixed frame (52) through a first connecting seat (554), one end of the second screw rod (552) is connected with an output shaft of the second driving motor (551), the other end of the second screw rod (552) penetrates through the first connecting seat (554) at the side far away from the second driving motor (551) and is rotatably connected with the first connecting seat (554).
5. The reagent strip filling device of claim 4, wherein: the longitudinal moving device (56) comprises a third driving motor (561), a third screw rod (562), a third guide rod (563) and second connecting seats (564), the two second connecting seats (564) are longitudinally arranged on the two transverse moving devices (55) respectively, the second screw rod (552) and the second guide rod (553) penetrate through the second connecting seats (564) respectively, the second connecting seats (564) are in threaded connection with the second screw rod (552), the second guide rod (553) is in sliding connection with the second connecting seats (564), the third driving motor (561) is connected with the second connecting seats (564), one end of the third screw rod (562) is connected with an output shaft of the third driving motor (561), the other end of the third screw rod (562) penetrates through the second connecting seats (564) far away from one side of the third driving motor (561), and is rotatably connected with the second connecting seats (564), and both ends of the third guide rod (563) are respectively connected with the two second connecting seats (564).
6. The reagent strip filling device according to claim 5, wherein: the third screw rod (562) and the third guide rod (563) respectively penetrate through the driving block (57), the driving block (57) is in threaded connection with the third screw rod (562), and the third guide rod (563) is in sliding connection with the driving block (57).
7. The reagent strip filling device according to claim 2, wherein: the filling part (58) comprises a fixing plate (581), a sleeve (582), a liquid flow limiting device (583), a clamping plate (584), a tapered sleeve (585) and a connecting rod (586), the fixing plate (581) is connected with the driving block (57), two ends of the sleeve (582) are closed, the sleeve (582) is arranged on the fixing plate (581), the liquid flow limiting device (583) is arranged inside the sleeve (582), one end of the sleeve (582) is connected with the sealing assembly (4) and the rear filling assembly (2) through a sleeve (3), the clamping plate (584) is connected with the other end of the sleeve (582) through the connecting rod (586), an annular groove (5841) is formed in the edge of the clamping plate (584), a vent hole (5842) is formed in the top of the clamping plate (584), a circular arc part (5843) is arranged on the bottom surface of the clamping plate (584), the conical sleeve (585) is disposed inside the vent hole (5842).
8. The reagent strip filling device of claim 7, wherein: the liquid flow limiting device (583) comprises a shell (5831), a sealing plug (5832), a limiting plate (5833) and a spring (5834), a flow guide cavity (58311) is arranged in the shell (5831), a sealing groove (58312) is formed in one side of the inner wall of the flow guide cavity (58311), a limiting groove (58313) is formed in the other side of the inner wall of the flow guide cavity (58311), one end of the sealing plug (5832) is inserted into the sealing groove (58312), the other end of the sealing plug (5832) is connected with the surface of the inner wall of the flow guide cavity (58311) through the spring (5834), the limiting plate (5833) is arranged on one side of the sealing plug (5832), the limiting plate (5833) is connected with the limiting groove (58313) in a sliding mode, and the other end of a liquid discharge pipe (217) is sequentially connected with the sleeve (3), the sleeve (582) and the shell (5831), The connecting rod (586) and the clamping plate (584) are communicated with the outside of the clamping plate (584).
9. The reagent strip filling apparatus according to claim 8, wherein: seal assembly (4) include air pump (41), trachea (42), pressure sensor (43), gasbag (44) and solenoid valve (45), gasbag (44) set up in the inside of ring channel (5841), air pump (41) set up in advance the opposite side of discharging liquid section of thick bamboo (214), the output of air pump (41) with the one end of trachea (42) is connected, the other end of trachea (42) runs through in proper order sleeve pipe (3), sleeve (582), connecting rod (586) and cardboard (584) with gasbag (44) intercommunication, pressure sensor (43) with solenoid valve (45) set up respectively on trachea (42), solenoid valve (45) link up one side of sleeve pipe (3) with the outside intercommunication of sleeve pipe (3).
10. A filling method using the reagent strip filling apparatus according to any one of claims 1 to 9, wherein: the method comprises the following steps:
s1, positioning the reagent strip, namely fixing the reagent strip (61) through a groove (621) formed in a fixing seat (62), and positioning the fixing seat (62) through a clamping groove (531) formed in a lifting plate (53);
s2, adjusting the position of the filling part, wherein the controller (7) controls the first driving motor (541), the second driving motor (551) and the third driving motor (561) to rotate respectively, so that the lifting plate (53) and the filling part (58) are positioned at initial positions, the initial positions are that the bottom of the lifting plate (53) is contacted with the top of the base (51), the position of the filling part (58) is positioned at an upper left corner, at the moment, the driving block (57) is contacted with the third driving motor (561), and the second connecting seat (564) is contacted with the second driving motor (551);
s3, supplementing a reagent, wherein a sealing plug (5832) is completely inserted into a sealing groove (58312) under the action of a spring (5834), the sealing plug (5832) seals a liquid discharge pipe (217) to prevent external air from entering the liquid discharge pipe (217), and a controller (7) controls a hydraulic cylinder (211) to drive a piston (213) to retract, so that the reagent communicated with the other end of a liquid inlet pipe (215) enters the liquid inlet and discharge barrel (214) through a one-way valve (216);
s4, sealing, wherein the controller (7) controls the second driving motor (551) and the third driving motor (561) to drive the filling part (58) to move to the upper side of the reagent strip (61) respectively, and controls the first driving motor (541) to insert six clamping plates (584) into the sample hole (611), the cleaning hole (612), the magnetic bead hole (613), the enzyme labeling hole (614), the substrate hole (615) and the TIP head cleaning hole (616) at the starting position respectively, the number of the sample hole (611), the cleaning hole (612), the magnetic bead hole (613), the enzyme labeling hole (614) and the TIP head cleaning hole (616) is three, the number of the substrate holes (615) is one, the controller (7) starts the air pump (41), and the air pump (41) supplies air to the air bag (44) through the air pipe (42) to expand the air bag (44) so as to keep the clamping plates (584) and the sample hole (611) and the sample hole (561), Sealing a cleaning hole (612), a magnetic bead hole (613), an enzyme labeling hole (614), a substrate hole (615) and a TIP head cleaning hole (616);
s5, reagent filling is performed for the first time, a controller (7) controls a hydraulic cylinder (211) to extend, a piston (213) extrudes the reagent in a liquid discharge cylinder (214), the reagent is respectively filled in a sample hole (611), a cleaning hole (612), a magnetic bead hole (613), an enzyme labeling hole (614), a substrate hole (615) and a TIP head cleaning hole (616) through a liquid discharge pipe (217) and a liquid flow limiting device (583), and air in the sample hole (611), the cleaning hole (612), the magnetic bead hole (613), the enzyme labeling hole (614), the substrate hole (615) and the TIP head cleaning hole (616) is discharged through a conical sleeve (585) in the filling process;
s6, the reagent flows back, after the pouring is finished, the controller (7) controls the hydraulic cylinder (211) to drive the piston (213) to retract, the reagent communicated with the other end of the liquid inlet pipe (215) enters the liquid inlet and outlet cylinder (214) through the one-way valve (216), in the retracting process of the piston (213), the reagent on one end of the liquid outlet pipe (217) positioned on the clamping plate (584) is sucked into the liquid outlet pipe (217), meanwhile, the sealing plug (5832) is inserted into the sealing groove (58312) under the action of the spring (5834) to seal the liquid outlet pipe (217) so as to avoid the reagent in the liquid outlet pipe (217) from completely flowing back into the liquid inlet and outlet cylinder (214), the controller (7) closes the air pump (41) and opens the electromagnetic valve (45) to exhaust air, then closes the electromagnet, and the air bag (44) contracts back to the inside of the annular groove (5841);
s7, filling the reagent for the second time, the controller (7) separating the clamping plate (584) from the reagent strip by controlling the first driving motor (541), moving the six clamping plates (584) of the filling part (58) to the upper side of the second sample hole (611), the second cleaning hole (612), the second magnetic bead hole (613), the second enzyme labeling hole (614) and the second TIP head cleaning hole (616) of the reagent strip (61) by controlling the second driving motor (551) and the third driving motor (561), inserting the six clamping plates (584) into the second sample hole (611), the second cleaning hole (612), the second magnetic bead hole (613), the second enzyme labeling hole (614) and the second TIP head cleaning hole (616) by controlling the first driving motor (7), starting the controller (41) by controlling the first driving motor (7), and inflating the air pump (41) to deliver air into the air bag (44) by the air pipe (42) to keep the air bag (584) and the air bag (541) and keep the air bag (44) inflated sample clamping plate (584) and the sample strip (61) and the air bag (541) and the air pump (41) inflated The hole (611), the cleaning hole (612), the magnetic bead hole (613), the enzyme labeling hole (614), the substrate hole (615) and the TIP head cleaning hole (616) are sealed;
s8, repeating the steps S5-S6;
s9, reagent is filled for the third time, the controller (7) controls the first driving motor (541) to separate the clamping plate (584) from the reagent strip, controls the second driving motor (551) and the third driving motor (561) to drive the six clamping plates (584) of the filling part (58) to move to the positions above the third sample hole (611), the third cleaning hole (612), the third magnetic bead hole (613), the third enzyme labeling hole (614) and the third TIP head cleaning hole (616) of the reagent strip (61), the controller (7) controls the first driving motor (613) to insert the six clamping plates (584) into the interiors of the third sample hole (611), the third cleaning hole (612), the third magnetic bead hole (613), the third enzyme labeling hole (614) and the third TIP head cleaning hole (616), the controller (7) starts the air pump (41), and the air pump (41) delivers air to the interior of the air bag (44) through the air pipe (42) to expand the air bag (44) so that the air bag (44) and the clamping plate (584) and the sample strip are kept by the air pump (41) The hole (611), the cleaning hole (612), the magnetic bead hole (613), the enzyme labeling hole (614), the substrate hole (615) and the TIP head cleaning hole (616) are sealed;
s10, repeating the steps S5-S6.
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Cited By (1)
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| CN117617598A (en) * | 2024-01-26 | 2024-03-01 | 宿迁市百亚电子商务有限公司 | Test method of electronic cigarette oiling component for electronic cigarette production |
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