CN115088528A - Octagonal seedling picking mechanism for octagonal anthracnose prevention - Google Patents
Octagonal seedling picking mechanism for octagonal anthracnose prevention Download PDFInfo
- Publication number
- CN115088528A CN115088528A CN202210791437.6A CN202210791437A CN115088528A CN 115088528 A CN115088528 A CN 115088528A CN 202210791437 A CN202210791437 A CN 202210791437A CN 115088528 A CN115088528 A CN 115088528A
- Authority
- CN
- China
- Prior art keywords
- box body
- aniseed
- anthracnose
- seedling
- octagonal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/14—Greenhouses
- A01G9/16—Dismountable or portable greenhouses ; Greenhouses with sliding roofs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/24—Devices or systems for heating, ventilating, regulating temperature, illuminating, or watering, in greenhouses, forcing-frames, or the like
- A01G9/247—Watering arrangements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of plant disease control, and discloses an octagonal seedling selecting mechanism for octagonal anthracnose prevention, which comprises a box body, wherein the box body comprises a bottom plate, a supporting plate and a cover plate, a plurality of stainless steel base plates are padded on the bottom plate, a plurality of supporting columns are fixedly connected to the bottom plate, a first air cylinder is arranged on the plurality of supporting columns, a cylinder body of the first air cylinder is fixedly connected with the supporting columns, an output shaft of the first air cylinder is fixedly connected with the cover plate, a control panel is arranged on the supporting plate, a temperature and humidity sensor, an intelligent temperature and humidity automatic controller and a bathroom heater are arranged on the control panel, the cover plate is connected to the box body in a covering mode, four lamp tubes are arranged on the cover plate and divided into two groups, atomizing nozzles are arranged on the cover plate and extend into the box body, the box body is enclosed by transparent greenhouse films except one side of the supporting plate, so that the rest surfaces of the box body are formed, and exhaust ports are arranged on the films. The invention has simple and light structure, can be moved to the place to be used at will, and can more conveniently and accurately select the needed octagonal anthracnose resistant seedlings.
Description
Technical Field
The invention relates to the technical field of plant disease control, in particular to an aniseed seedling selecting mechanism for preventing aniseed anthracnose.
Background
The star anise is a woody spice economic forest tree species with special characteristics and advantages in Guangxi, and is called as a main material in the north. The star anise product is widely applied to the fields of feed, chemical industry, food, medicine and daily necessities, is a traditional large export commodity in China, and has the largest planting area in Guangxi. The aniseed anthracnose is a main fungal disease on the aniseed trees, the disease causes abnormal fallen leaves, fallen flowers and fallen fruits of the aniseed trees, and serious causes the whole plant to die, which is a harmful piece and has great influence on the yield of the aniseed. The breeding and planting of disease-resistant varieties is an important means for preventing and treating crop diseases. The star anise has long service life, and investigation and research results show that the resistance of star anise forest stands to the diseases can be improved by selecting the strain with strong star anise anthracnose resistance for afforestation or crown changing grafting, so that the occurrence of the diseases is reduced, the use amount of pesticides is reduced, the related control cost is reduced, and the environment is protected. In addition, the strain afforestation with strong disease resistance has the function of continuously resisting the impregnation of pathogenic bacteria and playing a fundamental prevention and control role in the future growth process.
In order to effectively control the harm of the aniseed anthracnose, the aniseed seedlings suitable for producing the actual aniseed anthracnose-resistant aniseed strain are provided for social production, the aniseed seedlings are usually required to be selected, the actual aniseed anthracnose-resistant strain screening needs to be carried out on the forest land, and the screening process is complicated, long in time consumption, difficult and even impossible.
Disclosure of Invention
The invention aims to provide an aniseed seedling selecting mechanism for preventing aniseed anthracnose, and solves the problems that screening of aniseed anthracnose resistant seedlings in the prior art needs to be carried out on forest land, is complicated, time-consuming, difficult and even impossible to implement.
In order to achieve the above purpose, the basic scheme of the invention is as follows: the octagonal seedling selecting mechanism for the prevention of the octagonal anthracnose comprises a box body, wherein the box body comprises a bottom plate, a supporting plate and a cover plate, a plurality of stainless steel base plates are padded on the bottom plate, a plurality of supporting columns are fixedly connected to the bottom plate, a first air cylinder is arranged on the plurality of supporting columns, a cylinder body of the first air cylinder is fixedly connected with the supporting columns, an output shaft of the first air cylinder is fixedly connected with the cover plate, a control panel is arranged on the supporting plate, a temperature and humidity sensor, an intelligent temperature and humidity automatic controller and a bathroom heater are arranged on the control panel, the cover plate is covered on the box body, four lamp tubes are arranged on the cover plate, the lamp tubes are divided into two groups, one group has the wavelength of 365 and 500nm, the other group has the wavelength of 580 and 800nm, the two groups of lamp tubes are distributed at equal intervals along a diagonal line, atomizing nozzles are arranged on the cover plate and extend into the box body, the box body except one side of the supporting plate is enclosed by a transparent greenhouse film to form the rest surfaces of the box body, and the films are provided with exhaust ports.
Principle of the basic scheme: during operation, the devices are completely installed, then the seedlings are transplanted to the base plate after being punctured by aseptic plum blossom-shaped needles on two sides of a midvein in the middle of the seedling leaves, then the height of the cover plate is adjusted under the driving of the first air cylinder, the cover plate is adjusted to enable the distance between the lamp tube and the leaves at the top end of the seedlings to reach 80-90 centimeters, then the temperature and humidity sensor acquires the temperature and humidity information in the box body, the temperature and humidity sensor sends the information to the intelligent temperature and humidity automatic controller, the intelligent temperature and humidity automatic controller controls the bathroom heater and the driving piece of the atomizing nozzle according to the received information so as to adjust the temperature and humidity, the temperature is maintained between 28 ℃ and 32 ℃, the relative humidity is maintained at 80-90%, and then the conidium liquid for inoculation is prepared into 5 × 104 spores/ml by adopting a plant pathology technical method, and opening a spray head to spray moisture on the leaf surfaces of the seedlings for inoculation, powering on and turning on a lamp, keeping illumination for 24 hours, maintaining for 15 days, and taking out the seedlings, thereby investigating the disease index and disease resistance evaluation of the seedlings.
The advantages of the basic scheme are: under the action of the first cylinder, the height distance between the lamp tube and the seedlings can be freely and flexibly adjusted to reach the required height, so that the seedlings are irradiated in a proper distance, then the temperature and humidity information in the box body is obtained by the temperature and humidity sensor and is sent to the intelligent temperature and humidity automatic controller, the intelligent temperature and humidity automatic controller adjusts the bathroom heater and the first pump assembly according to the received information, so that the humidity and the temperature in the box body reach the required quantity, the seedlings can be in a better proper environment for subsequent illumination operation, after the illumination is finished, the disease index and the disease resistance evaluation of the seedlings are checked, so that the more proper seedlings can be selected, the mechanism is simple and easy to configure, can be assembled and is flexible and convenient to operate, a certain number of seedlings resistant to the aniseed anthracnose can be selected in a short time, and the trouble that the traditional screening of the seedlings resistant to the aniseed anthracnose needs to be carried out on the forest land is compensated to a certain extent, time consumption, difficulty, high cost and the like.
Further, atomizer includes inner tube and outer tube, the outer tube top is equipped with the second cylinder, second cylinder body and apron fixed connection, second cylinder output shaft and outer tube fixed connection, be equipped with a plurality of atomizing orifices on the inner tube, inner tube and outer tube sliding fit, be equipped with the pivot in the inner tube, a plurality of first dead levers of fixedly connected with in the pivot, first dead lever and inner tube fixed connection are passed through in the pivot, the atomizer top is equipped with annular case, annular case and atomizer intercommunication, annular case and apron fixed connection, the annular case top is equipped with the motor, the motor both sides are equipped with the second dead lever, the motor passes through second dead lever and cover connection, the coaxial fixed connection pivot of output shaft of motor, annular case and motor normal running fit, annular case one side has first pump package spare through the connecting pipe intercommunication.
Principle of the basic scheme: under the drive of first pump package spare, the liquid that will need to pour into passes through annular case input to atomizer department, then under the drive of motor, will drive the shower nozzle and carry out rotary motion, then required spun liquid sprays around in toward the case body through a plurality of atomizing orifices to under the drive of second cylinder, the outer tube carries out vertical motion, the water yield and the height of adjustable shower nozzle from this.
The advantages of the basic scheme are: the atomizing nozzle can be driven to rotate under the driving of the motor, so that the sprayed liquid can spray sterile water or spore liquid to the periphery along with the rotating motion, thereby, when adjusting the humidity, the inside of the box body can be fully sprayed by the sterile water, thereby the required humidity can be adjusted more quickly, when the spore liquid is used for spraying the seedlings, the seedlings in the box body can be sprayed in all directions, so as to avoid that partial seedlings can not be fully sprayed due to being positioned at the dead angle in the box body, and simultaneously, the outer pipe is driven by the second air cylinder to move vertically, therefore, the water yield can be adjusted, when the outer tube slides to a certain distance, the outer tube can cover the atomization spray holes of the cover part, therefore, the liquid amount sprayed out can be reduced, and when the atomization spray holes of the inner tube are not covered, the maximum liquid amount can be sprayed out so as to adjust the proper flow rate.
Further, a plurality of universal wheels are arranged at the bottom of the box body.
Principle of the basic scheme: through the design of universal wheel, this mechanism of removal that can be comparatively convenient.
The advantages of the basic scheme are: this mechanism passes through the design of universal wheel, removal at will to people can move this mechanism to different places and carry out work when carrying out the operation of selecting of reality, consequently can make the staff use more convenient nimble, remedied the defect in prior art in a certain sense, improved staff's work efficiency, the user demand of more suitable reality.
Further, one side that control panel was kept away from to the backup pad is equipped with the second pump subassembly, and the second pump subassembly passes through the connecting pipe intercommunication gas vent.
Principle of the basic scheme: the gas entering the tank body is exhausted through the exhaust port by the driving of the second pump assembly.
The advantages of the basic scheme are: with this internal gas outgoing of case to build suitable environment for the seedling, and through the design of second pump package spare, make the gas can be more convenient nimble by the discharge.
Further, a venturi tube is arranged below the control panel, an air amplifier is arranged at one end of the venturi tube, a throat section of the venturi tube is communicated with an air outlet at one side of the box body through a connecting pipe, and an expansion section of the venturi tube is communicated with an air outlet at the other side of the box body through a connecting pipe.
Principle of the basic scheme: input gas into the air amplifier through the injector, when gas flows to the air amplifier, high-pressure gas passing through the air amplifier drives gas around the input end of the air amplifier, thereby outputting a large amount of low-pressure gas flow at the output end of the air amplifier at a high speed, the flowing amount of the gas is amplified to multiple times, then when the gas passes through the throat section of the venturi tube, the throat section is in a negative pressure state, then the gas in the box body is extracted through the connecting pipe, when the extracted gas flows to the expansion section of the venturi tube, part of the gas flows to the box body through the connecting pipe.
The advantages of the basic scheme are: the design of the air amplifier can lead the gas flow to reach multiple times, thus leading the throat section of the venturi tube to be in a negative pressure state and continuously extracting the gas in the box body, leading part of the gas to continuously flow into the box body through the expansion section of the venturi tube, thus leading the gas in the box body to be extracted more mildly on one hand and leading the gas in the box body to be continuously and circularly updated through the circular flow of the gas on the other hand.
Further, the mechanism is used in the following way,
s1, connecting and mounting all the components of the mechanism completely,
s2, moving the nursery stock to be selected and wound on the leaf to a backing plate in the mechanism,
s3, adjusting the length of the four-corner support column to make the distance between the lamp tube on the top supporting plate and the leaves at the top of the nursery stock reach 80-90 cm,
s4, adjusting a temperature and humidity controller, keeping the temperature between 28 and 32 ℃ and the relative humidity between 80 and 90 percent,
s5, opening the spray head to spray the star anise anthracnose spore suspension with the concentration of 5 x 104 spores/ml on the leaf surface of the seedling for inoculation, spraying until the dripping degree is reached,
s6, turning on the lamp, keeping 24 hours illumination, keeping for 15 days,
and S7, taking out the seedlings, and investigating disease index and disease resistance evaluation.
Drawings
FIG. 1 is a front view of an octagonal seedling picking mechanism for octagonal anthracnose prevention in an embodiment of the invention.
FIG. 2 is a front view of an octagonal seedling picking mechanism for octagonal anthracnose prevention in an embodiment of the invention.
FIG. 3 is a front view of the support plate of the octagonal seedling picking mechanism for octagonal anthracnose prevention in the embodiment of the invention.
FIG. 4 is a top view of the atomizer of the anise seedling selection mechanism for anise anthracnose prevention in the embodiment of the present invention.
FIG. 5 is a front view of a support plate of an octagonal seedling picking mechanism for octagonal anthracnose prevention in yet another embodiment of the invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the box comprises a box body 1, a support column 2, a cover plate 3, a lamp tube 4, an exhaust hole 5, a first air cylinder 6, a second pump assembly 7, a universal wheel 8, a bottom plate 9, a first pump assembly 10, a motor 12, an annular box 13, an atomizing nozzle 14, a rotating shaft 15, a control panel 16, an intelligent temperature and humidity automatic controller 17, a bathroom heater 18, a temperature and humidity sensor 19, a support plate 20, a venturi tube 22, an air amplifier 23, an outer tube 24, an inner tube 25 and a first fixing rod 26.
The embodiment is substantially as shown in figures 1, 2 and 3 of the accompanying drawings: a octagonal seedling selecting mechanism for octagonal anthracnose prevention comprises a box body 1, wherein the bottom of the box body 1 is provided with a plurality of universal wheels 8, the box body 1 comprises a bottom plate 9, a supporting plate 20 and a cover plate 3, a plurality of stainless steel backing plates are padded on the bottom plate 9, a plurality of supporting columns 2 are fixedly connected to the bottom plate 9, a first air cylinder 6 is arranged on the plurality of supporting columns 2, a cylinder body of the first air cylinder 6 is fixedly connected with the supporting column 2, an output shaft of the first air cylinder 6 is fixedly connected with the cover plate 3, the bottom plate 9 is fixedly connected with the supporting plate 20, a control panel 16 is arranged on the supporting plate 20, a temperature and humidity sensor 19, an intelligent temperature and humidity automatic controller 17 and a bath heater 18 are arranged on the control panel 16, the cover plate 3 is connected to the box body 1, four lamp tubes 4 are arranged on the cover plate 3, the lamp tubes 4 are divided into two groups, one group has a wavelength of 365 and 500nm, the other group has a wavelength of 580 and 800nm, and the two groups of lamp tubes 4 are equidistantly distributed along a diagonal line, a second pump assembly 7 is arranged on one side of the support plate 20 far away from the control panel 16, the second pump assembly 7 is communicated with the exhaust port through a connecting pipe, the atomizer 14 comprises an inner pipe 25 and an outer pipe 24, a second cylinder is arranged on the top of the outer pipe 24, the cylinder body of the second cylinder is fixedly connected with the cover plate 3, the output shaft of the second cylinder is fixedly connected with the outer pipe 24, a plurality of atomizing spray holes are arranged on the inner pipe 25, the inner pipe 25 is in sliding fit with the outer pipe 24, a rotating shaft 15 is arranged in the inner pipe 25, a plurality of first fixing rods 26 are fixedly connected on the rotating shaft 15, the rotating shaft 15 is fixedly connected with the inner pipe 25 through the first fixing rods 26, an annular box 13 is arranged on the top of the atomizer 14, the annular box 13 is communicated with the atomizer 14, the annular box 13 is fixedly connected with the cover plate 3, a motor 12 is arranged on the top of the annular box 13, second fixing rods are arranged on two sides of the motor 12, the motor 12 is connected with the cover plate 3 through the second fixing rods, the output shaft of the motor 12 is coaxially and fixedly connected with the rotating shaft 15, annular case 13 and motor 12 normal running fit, annular case 13 one side has first pump package spare 10 through the connecting pipe intercommunication, and case body 1 removes backup pad 20 one side and all encloses with the transparent greenhouse film and seal to form case body 1 remaining face, and all be equipped with the gas vent on the film.
The specific implementation process is as follows: (1) during operation, the devices are completely installed, then the seedlings are transplanted to the backing plate after being punctured by aseptic plum blossom-shaped needles on two sides of the midvein in the middle of the seedling leaves, then the height of the cover plate 3 is adjusted under the driving of the first air cylinder 6, the distance between the lamp tube 4 and the leaves at the top end of the seedlings reaches 80-90 centimeters, then the temperature and humidity sensor 19 acquires the temperature and humidity information in the box body 1, the temperature and humidity sensor 19 sends the information to the intelligent temperature and humidity automatic controller 17, the intelligent temperature and humidity automatic controller 17 controls the bathroom heater 18 and the first pump assembly 10 according to the received information so as to adjust the temperature and humidity, the temperature is maintained between 28 ℃ and 32 ℃, the relative humidity is maintained between 80-90%, and then the conidium liquid for inoculation is prepared into the concentration of 5 x 104 spores/ml by adopting a plant pathology technical method, and opening a spray head to spray moisture on the leaf surfaces of the seedlings for inoculation, powering on and turning on a lamp, keeping illumination for 24 hours, maintaining for 15 days, and taking out the seedlings, thereby investigating the disease index and disease resistance evaluation of the seedlings.
(2) Under the effect of first cylinder 6, can be free nimble height distance of regulation fluorescent tube 4 and seedling, thereby reach required height, so that the seedling is shone in suitable distance, then under humiture inductor 19 and humiture intelligent automatic control 17 cooperation, temperature and humidity in the adjustable case body 1 make the seedling carry out subsequent illumination operation in a better environment, the state of an illness index and the disease resistance evaluation of seedling of the convenient investigation after the illumination is accomplished, thereby can select more suitable octagon seedling, and under the drive of motor 12, can drive atomizer 14 and carry out rotary motion, thereby make spun liquid along with rotary motion around spray sterile water or spore liquid, thereby when adjusting humidity, can make the inside abundant sterile water that is sprayed to of case body 1, consequently can adjust required humidity more rapidly, when using spore liquid to spray the seedling, can carry out the omnidirectional injection to the inside seedling of case body 1, in order to avoid partial seedling to be because of being in case body 1 internal dead angle department and can not be arrived by abundant injection, simultaneously under the drive of second cylinder, the outer tube carries out vertical motion, the water yield of adjustable atomizer from this, when the outer tube slides certain distance, outer tube 24 can hide partial atomizing orifice, thereby reducible spun liquid volume, when the atomizing orifice of inner tube 25 is not hidden, alright the biggest liquid volume of blowout, in order to adjust suitable flow. In addition, the mechanism can be freely moved through the design of the universal wheels 8, so that when people perform actual selection operation, the mechanism can be moved to different places for working, therefore, the mechanism can be more convenient and flexible for workers to use, overcomes the defects in the prior art to a certain extent, improves the working efficiency of the workers, is more suitable for actual use requirements, is simple and easy to configure, can be assembled and is flexible and convenient to operate, can select a certain number of seedlings resisting the aniseed anthracnose in a short time, and accordingly compensates the trouble that the traditional selection of the seedlings resisting the aniseed anthracnose needs to be carried out on the forest land to work to a certain extent, time consumption, difficulty, high cost and the like.
In the present embodiment, the mechanism is used in the following manner,
s1, connecting and installing all the components of the mechanism completely,
s2, moving the nursery stock to be selected and wound on the leaf to a backing plate in the mechanism,
s3, adjusting the length of the four-corner post 2 to make the distance between the lamp tube 4 on the top supporting plate 20 and the leaves at the top of the nursery stock reach 80-90 cm,
s4, adjusting the temperature and humidity of the intelligent automatic controller 17, adjusting the temperature to 28-32 ℃, keeping the relative humidity to 80-90%,
s5, opening the spray head 14 to spray the star anise anthracnose spore suspension with the concentration of 5 × 104 spores/m 1 on the leaf surface of the seedling for inoculation until the dropping water degree,
s6, turning on the lamp, keeping 24 hours illumination, keeping for 15 days,
and S7, taking out the seedlings, and investigating disease index and disease resistance evaluation.
The present application further differs from the above embodiments in that, basically as shown in fig. 4 and fig. 5, a venturi 22 is provided below the control panel 16, an air amplifier 23 is provided at one end of the venturi 22, a throat section of the venturi 22 is connected to an air outlet at one side of the box body 1 through a connecting pipe, and an expansion section of the venturi 22 is connected to an air outlet at the other side of the box body 1 through a connecting pipe.
In this embodiment, gas is input into the air amplifier 23 through the injector, when the gas flows to the air amplifier 23, the high-pressure gas passing through the air amplifier 23 drives the gas around the input end of the air amplifier 23, so as to output a large amount of low-pressure gas flow at a high speed at the output end of the air amplifier 23, at this time, the flow amount of the gas is amplified to multiple times, then when the gas passes through the throat section of the venturi 22, the throat section is in a negative pressure state, then the gas in the box body 1 is extracted through the connecting pipe, when the extracted gas flows to the expansion section of the venturi 22, part of the gas flows into the box body 1 through the connecting pipe, so that on one hand, the gas in the box body 1 can be extracted more gently, and on the other hand, the gas in the box body 1 can be continuously and circularly updated through the circular flow of the gas.
And (3) experimental monitoring results:
example 3
In the star anise forests in different areas, according to the disease characteristics of star anise anthracnose, a disease spot area ratio method (the ratio of disease spot area to leaf area) is adopted as a disease grading standard, branches of plants with different disease-sensitive indexes (different resistance grades) are selected to be grafted to breed seedlings, then conidium solutions with different concentrations are respectively adopted in nursery lands to inoculate the seedlings, and the resistance of the seedlings to the star anise anthracnose is investigated on the 15 th day after inoculation. (test time: 6-9 months, 3 repetitions, and average values of the measured values.) the test results are shown in Table 1.
Table 1 different spore concentration results units for seedling inoculation: spores/ml%
| Concentration of spore liquid | Index of inter-forest disease | Determination of disease index | The goodness of fit of the two compositions is% |
| 5×10 2 | 48.7 | 4.9±0.05 | 10.1C |
| 5×10 3 | 48.7 | 12.5±0.21 | 25.6B |
| 5×10 4 | 48.7 | 26.9±0.25 | 55.2A |
| 5×10 5 | 48.7 | 27.1±0.31 | 55.6A |
| 5×10 6 | 48.7 | 27.2±0.52 | 55.8A |
| 5×10 7 | 48.7 | 26.9±0.28 | 55.3A |
The test result shows that: the pathogenicity of different spore liquid concentrations is different, and the inoculation result with the concentration of 5 multiplied by 104-5 multiplied by 107 spores/ml has higher coincidence rate with the actual result, but not more than 56%. The different capital letters in the table indicate that the differences are very significant (same below).
Example 4
Referring to example 3, seedlings were inoculated with different inocula. Disease indices of seedlings were investigated on day 15. The test results are shown in Table 2.
TABLE 2 results of the determination of the resistance of different inoculants to seedlings
The test result shows that: the pathogenicity of different inoculants was different, and the closeness of the resistance determined with mycelial patch inoculation to the actual was relatively high, up to 75.1%.
Example 5
Referring to example 3, after inoculation of seedlings, the seedlings were subjected to pathogenic induction in chambers of different temperature ranges. Disease indices of seedlings were investigated on day 15. The test results are shown in Table 3.
Table 3 measurement of seedling resistance under different temperature conditions units: c
The test result shows that: after inoculation, the seedlings were exposed to different temperature ranges, and the measured resistance was relatively close to the actual one when inoculated at a temperature range of 26-32 ℃.
Example 6
Referring to example 3, after inoculation of seedlings, the seedlings were subjected to pathogenic induction in an environment of different humidity ranges. Disease indices of seedlings were investigated on day 15. The test results are shown in Table 4.
Table 4 results units of the determination of the resistance of seedlings under different humidity conditions: by%
The test shows that: seedlings with high humidity are prone to disease. The test results showed that the measured resistance was relatively close to the actual value when inoculated in the range of 71% to 90% relative humidity.
Example 7
After the seedling inoculation, the seedling is subjected to pathogenic induction in the environment of the combined factors as described in the specification 1 and the specification 2. Disease index of seedlings was investigated after 15 days. The test results are shown in Table 5.
Table 5 results units for different combinations of induction factors for seedling inoculation: is based on
| Combination of inducing factors | Index of inter-forest disease | Determination of disease index | The goodness of fit of the two compositions is% | Remarks for note |
| I | 48.7 | 40.9±0.96 | 84.0B | See description 1 |
| II | 48.7 | 50.5±0.26 | 96.4A | |
Description 1: the inoculum is conidium liquid cultured by PDA culture medium with concentration of 5 × 104 spores/ml, after wound is caused to seedling leaf, the spraying method is used for inoculation, and after inoculation, the seedling is in: inducing pathogenicity under natural environment conditions with the temperature of 26-32 ℃ and the relative humidity of 75-85 percent.
Description 2: it is the content of the present invention. The inoculum was conidia broth cultured in PLNA sporulation medium at a concentration of 5 × 104 spores/ml. After wound is caused to the leaf blade of the seedling, the seedling is inoculated by a spraying method, and after inoculation, the seedling is positioned in the following position: the temperature is 28-32 ℃, the relative humidity is 80-90%, and the pathogenicity is induced under the space environment irradiated by the lamp light in 24 hours (the lamp light wavelength is 365-.
The test time is 8 months, and the period is the peak period of the onset of aniseed anthracnose in the forest.
The test result shows that: compared with the inducing factor combination I (the inducing factor combination I is similar to a commonly used disease resistance identification method of the common technicians in the field for anthracnose of crops such as soybeans, eggplants, peppers, mango trees and the like), the disease index of seedlings of the inducing factor combination II is more consistent with the disease index of actual forest lands, and the consistency reaches 96.4 percent. The difference between the two reaches the level of extremely significant difference.
Example 8
On the basis of the embodiments 1 to 8, the method carries out field verification test again, the time period of the field verification test is 2 years, and the specific time is selected from 6-9 months of the onset peak of the anthracnose of the aniseed every year.
The following are specific experimental data and results:
in the test, seedlings with different resistance levels selected by the invention are transplanted into illicium verum woodlands in different slopes, spores in a PLNA spore production culture medium are prepared into spore suspension with the concentration of 5 x 104 spores/ml in 6-9 months of the outbreak peak period of the anthracnose of the illicium verum in the second and third years, the spore suspension is used for spot-coating inoculation after the leaves are punctured with wounds, and the leaves are sprayed with sterile water once every 10 hours, 13 hours and 18 hours after the inoculation, and the continuous period lasts for 10 days. After 30 days, the disease index of the seedlings was investigated. The test was run in a random train arrangement, repeated three times.
The test result shows that: the resistance level of the selected seedlings in the forest is matched with the resistance level selected and evaluated by the method, and the disease index goodness of fit of the resistance level and the resistance level is between 85 and 95 percent. Shows that: the resistance level of the seedlings selected by the aniseed seedling selecting mechanism for preventing the aniseed anthracnose is consistent with the actual resistance level of the forest land, namely the invention has stronger practicability.
The results of the tests are detailed in table 6 below.
TABLE 6 comparison of resistance of selected seedlings in field test according to the invention
As fully demonstrated in examples 1-8 above, compared with the method for identifying similar anthracnose of crops such as soybean, eggplant, pepper, mango and the like by a person skilled in the art, star anise is a perennial woody aromatic plant and has self-specific biological characteristics. According to the aniseed seedling selecting mechanism for aniseed anthracnose prevention, the anthracnose resistance of the aniseed seedlings is determined, the selecting result is more consistent with the actual morbidity situation in a forest, and the determining result achieves an unexpected better effect.
The present invention has been described in general terms, but it will be apparent to those skilled in the art that modifications and improvements can be made based on the present invention. Therefore, it is possible to modify or improve the optical characteristics of the optical fiber without departing from the spirit of the present invention.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures and/or features of the schemes is not described herein in excess. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (6)
1. A mechanism is selected with aniseed seedling for aniseed anthracnose prevention, its characterized in that: comprises a box body, the box body comprises a bottom plate, a supporting plate and a cover plate, a plurality of stainless steel backing plates are padded on the bottom plate, a plurality of pillars are fixedly connected on the bottom plate, a first air cylinder is arranged on the pillars, the cylinder body of the first air cylinder is fixedly connected with the pillars, the output shaft of the first air cylinder is fixedly connected with the cover plate, the bottom plate is fixedly connected with the supporting plate, a control panel is arranged on the supporting plate, a temperature and humidity sensor is arranged on the control panel, humiture intelligence automatic control and bathroom heater, apron lid connect on the case body, are equipped with four fluorescent tubes on the apron, and the fluorescent tube divide into two sets ofly, and a set of wavelength is 365-.
2. The aniseed seedling picking mechanism for aniseed anthracnose prevention according to claim 1, characterized in that: atomizer includes inner tube and outer tube, the outer tube top is equipped with the second cylinder, second cylinder body and apron fixed connection, second cylinder output shaft and outer tube fixed connection, be equipped with a plurality of atomizing orifices on the inner tube, inner tube and outer tube sliding fit, be equipped with the pivot in the inner tube, a plurality of first dead levers of fixedly connected with in the pivot, first dead lever and inner tube fixed connection are passed through in the pivot, the atomizer top is equipped with annular case, annular case and atomizer intercommunication, annular case and apron fixed connection, annular roof portion is equipped with the motor, the motor both sides are equipped with the second dead lever, the motor passes through second dead lever and apron connection, the coaxial fixed connection pivot of output shaft of motor, annular case and motor normal running fit, annular case one side has first pump package spare through the connecting pipe intercommunication.
3. The aniseed seedling picking mechanism for aniseed anthracnose prevention according to claim 2, characterized in that: the bottom of the box body is provided with a plurality of universal wheels.
4. The aniseed seedling picking mechanism for aniseed anthracnose prevention according to claim 3, characterized in that: one side that control panel was kept away from to the backup pad is equipped with the second pump subassembly, and the second pump subassembly passes through the connecting pipe intercommunication gas vent.
5. The mechanism is selected with aniseed seedling to be used for star anise anthracnose prevention according to claim 3, characterized in that: a venturi is arranged below the control panel, an air amplifier is arranged at one end of the venturi, a throat section of the venturi is communicated with an air outlet at one side of the box body through a connecting pipe, and an expansion section of the venturi is communicated with an air outlet at the other side of the box body through a connecting pipe.
6. The aniseed seedling picking mechanism for aniseed anthracnose prevention according to claim 5, characterized in that: the method of use of the mechanism is as follows,
s1, connecting and mounting all the components of the mechanism completely,
s2, moving the nursery stock to be selected and wound on the leaf to a backing plate in the mechanism,
s3, adjusting the length of the four-corner support column to make the distance between the lamp tube on the top supporting plate and the leaves at the top of the nursery stock reach 80-90 cm,
s4, adjusting the temperature and humidity of the intelligent automatic controller, adjusting the temperature to be between 28 and 32 ℃, keeping the relative humidity to be between 80 and 90 percent,
s5, opening a spray nozzle to spray the octagonal anthracnose spore suspension with the concentration of 5 × 104 spores/ml on the leaf surface of the seedling for inoculation, spraying until the dripping degree is reached,
s6, turning on the lamp, keeping 24 hours illumination, keeping for 15 days,
and S7, taking out the nursery stock, and investigating disease index and disease resistance evaluation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210791437.6A CN115088528A (en) | 2022-07-05 | 2022-07-05 | Octagonal seedling picking mechanism for octagonal anthracnose prevention |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210791437.6A CN115088528A (en) | 2022-07-05 | 2022-07-05 | Octagonal seedling picking mechanism for octagonal anthracnose prevention |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115088528A true CN115088528A (en) | 2022-09-23 |
Family
ID=83296467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210791437.6A Pending CN115088528A (en) | 2022-07-05 | 2022-07-05 | Octagonal seedling picking mechanism for octagonal anthracnose prevention |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115088528A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10210869A (en) * | 1996-11-29 | 1998-08-11 | Nippon Glass Kogyo Kk | Plant pathogenic fungus inoculator |
| CN103314697A (en) * | 2013-07-01 | 2013-09-25 | 张振华 | Water-fertilizer-gas integrated irrigation control system and control method |
| CN206181975U (en) * | 2016-10-19 | 2017-05-24 | 宁波大叶园林科技有限公司 | Spray line with adjustable spray angle |
| CN109819800A (en) * | 2019-03-07 | 2019-05-31 | 胡海航 | A kind of light source regulating device of nursery box |
| CN211931835U (en) * | 2020-01-12 | 2020-11-17 | 南安市跃启机械贸易有限公司 | Novel agricultural irrigation sprays device |
| CN212594698U (en) * | 2020-06-28 | 2021-02-26 | 武蒙 | Building site dust fall equipment convenient to remove |
| CN112568092A (en) * | 2019-09-27 | 2021-03-30 | 邱建荣 | Watering device for fruit and vegetable planting |
| CN213095162U (en) * | 2020-07-21 | 2021-05-04 | 杨颖� | Seedling raising box for forestry greening engineering |
| CN213404268U (en) * | 2020-10-09 | 2021-06-11 | 江汉大学 | Pepper in-vitro fruit inoculation anthracnose resistance determination device |
| CN214508277U (en) * | 2020-11-12 | 2021-10-29 | 龙岩市万花园林有限公司 | Butterfly orchid breeding device |
-
2022
- 2022-07-05 CN CN202210791437.6A patent/CN115088528A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10210869A (en) * | 1996-11-29 | 1998-08-11 | Nippon Glass Kogyo Kk | Plant pathogenic fungus inoculator |
| CN103314697A (en) * | 2013-07-01 | 2013-09-25 | 张振华 | Water-fertilizer-gas integrated irrigation control system and control method |
| CN206181975U (en) * | 2016-10-19 | 2017-05-24 | 宁波大叶园林科技有限公司 | Spray line with adjustable spray angle |
| CN109819800A (en) * | 2019-03-07 | 2019-05-31 | 胡海航 | A kind of light source regulating device of nursery box |
| CN112568092A (en) * | 2019-09-27 | 2021-03-30 | 邱建荣 | Watering device for fruit and vegetable planting |
| CN211931835U (en) * | 2020-01-12 | 2020-11-17 | 南安市跃启机械贸易有限公司 | Novel agricultural irrigation sprays device |
| CN212594698U (en) * | 2020-06-28 | 2021-02-26 | 武蒙 | Building site dust fall equipment convenient to remove |
| CN213095162U (en) * | 2020-07-21 | 2021-05-04 | 杨颖� | Seedling raising box for forestry greening engineering |
| CN213404268U (en) * | 2020-10-09 | 2021-06-11 | 江汉大学 | Pepper in-vitro fruit inoculation anthracnose resistance determination device |
| CN214508277U (en) * | 2020-11-12 | 2021-10-29 | 龙岩市万花园林有限公司 | Butterfly orchid breeding device |
Non-Patent Citations (1)
| Title |
|---|
| 曾照芳 等: "茶叶质量安全控制与溯源", 西南交通大学出版社, pages: 297 - 194 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| de Oliveira et al. | Yield of micropropagated sugarcane varieties in different soil types following inoculation with diazotrophic bacteria | |
| Bulger et al. | Influence of temperature and wetness duration on infection of strawberry flowers by Botrytis cinerea and disease incidence of fruit originating from infected flowers. | |
| Weathers et al. | Aeroponics for the culture of organisms, tissues and cells | |
| WO2023025115A1 (en) | Application of 2-amino-3-methylhexanoic acid in promoting plant growth and increasing yield | |
| Utkhede | Increased growth and yield of hydroponically grown greenhouse tomato plants inoculated with arbuscular mycorrhizal fungi and Fusarium oxysporum f. sp. radicis-lycopersici | |
| Delhomez et al. | Susceptibility of strawberry cultivars and advanced selections to leaf spot caused by Mycosphaerella fragariae | |
| CN115088528A (en) | Octagonal seedling picking mechanism for octagonal anthracnose prevention | |
| Ahmed et al. | Physiologic specialisation in Macrophomina phaseoli (Maubl.) Ashby., causing stem rot of jute, Corchorus species | |
| El-Borhamy et al. | Physiological, Biochemical and Agronomic response of some flax cultivars to water deficit under clay soil conditions in North Delta | |
| CN104974939A (en) | Fusarium equiseti strain for preventing and treating poplar canker and application thereof | |
| CN103937684A (en) | Grass-inhibiting fungi screened from eucalyptus soil | |
| Dechassa et al. | Survey of Tea (Camellia sinensis) Diseases in Southwestern Ethiopia | |
| Amani et al. | Isolation and identification of fungal pathogens on banana trees (Musa acuminata L.) in Iran. | |
| Urazaliev et al. | Somaclonal variation of sugar beet resistant to pathogenic root rot Fusarium oxysporum var. orthoceras | |
| Keitt | Inoculation experiments with species of Coccomyces from stone fruits | |
| CN108770682B (en) | Wheat breeding method for resistance to snow mold leaf blight | |
| Page | Botrytis leaf spot on onions and its control | |
| Ahmed et al. | Studies of Botrytis cinerea causing botrytis gray mold disease in chickpea (Cicer arietinum L.) | |
| CN108738901A (en) | A kind of medicinal material seedling culture room | |
| Oliveira et al. | Selection of teak clones resistant to the fungus Ceratocystis fimbriata. | |
| Lisboa et al. | Development and physiological aspects of three species of passion fruit submitted to water stress | |
| Zeynalova | Study of sowing quality, oiliness and resistance to wilt disease of seeds of geographically distant cotton varieties | |
| Belachew et al. | Study on coffee wilt diseases (Gibberella xylarioides heim and saccus) resistant coffee genotypes at Jimma, southwest Ethiopia | |
| Purnami et al. | Identification of Pests and Diseases in Coffee Plants in the Manggarai Area–Flores, Indonesia | |
| Dar et al. | Status, symptomatology and partial characterization of stem bark canker disease of apple (Malus domestica Borkh.) in Kashmir Valley |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |