CN115078615A - Extraction column for liquid chromatography - Google Patents
Extraction column for liquid chromatography Download PDFInfo
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- CN115078615A CN115078615A CN202211002349.XA CN202211002349A CN115078615A CN 115078615 A CN115078615 A CN 115078615A CN 202211002349 A CN202211002349 A CN 202211002349A CN 115078615 A CN115078615 A CN 115078615A
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- 238000000605 extraction Methods 0.000 title claims abstract description 57
- 238000004811 liquid chromatography Methods 0.000 title claims abstract description 15
- 239000012528 membrane Substances 0.000 claims abstract description 46
- 229920000642 polymer Polymers 0.000 claims abstract description 34
- 239000011148 porous material Substances 0.000 claims abstract description 22
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 16
- 239000004698 Polyethylene Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 8
- -1 polyethylene Polymers 0.000 claims description 8
- 239000004743 Polypropylene Substances 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- 229920001155 polypropylene Polymers 0.000 claims description 5
- 229920000573 polyethylene Polymers 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 239000004695 Polyether sulfone Substances 0.000 claims description 2
- 229920006393 polyether sulfone Polymers 0.000 claims description 2
- 239000011347 resin Substances 0.000 claims description 2
- 229920005989 resin Polymers 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 2
- 229920002799 BoPET Polymers 0.000 claims 1
- 239000005041 Mylar™ Substances 0.000 claims 1
- 239000004677 Nylon Substances 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 claims 1
- 229920001778 nylon Polymers 0.000 claims 1
- 230000003670 easy-to-clean Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 24
- 239000012071 phase Substances 0.000 description 19
- 239000012535 impurity Substances 0.000 description 13
- 239000000463 material Substances 0.000 description 12
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
- 238000004140 cleaning Methods 0.000 description 9
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical group OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
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- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000004696 Poly ether ether ketone Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 229920000620 organic polymer Polymers 0.000 description 3
- 229920002530 polyetherether ketone Polymers 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- NIJJYAXOARWZEE-UHFFFAOYSA-N Valproic acid Chemical compound CCCC(C(O)=O)CCC NIJJYAXOARWZEE-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
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- 229920002521 macromolecule Polymers 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 229920005597 polymer membrane Polymers 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000004780 2D liquid chromatography Methods 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
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- 239000012472 biological sample Substances 0.000 description 1
- FFGPTBGBLSHEPO-UHFFFAOYSA-N carbamazepine Chemical compound C1=CC2=CC=CC=C2N(C(=O)N)C2=CC=CC=C21 FFGPTBGBLSHEPO-UHFFFAOYSA-N 0.000 description 1
- 229960000623 carbamazepine Drugs 0.000 description 1
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- 230000008602 contraction Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 229920006284 nylon film Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920006267 polyester film Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
- 229960000604 valproic acid Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention provides an extraction column for liquid chromatography, which takes an inflow end of a mobile phase as an upper end, and comprises a column cap, a front-end column cap, a column tube, a tail-end column cap and a column cap which are sequentially connected from top to bottom, wherein a stationary phase is filled in the column tube, the upper end of the front-end column cap is provided with a pressure-variable pore structure, and the pressure-variable pore structure comprises an upper layer, a middle layer and a lower layer, and sequentially comprises an elastic soft plate, a polymeric filter membrane and a supporting pore plate from top to bottom; the stationary phase is provided with a polymer liquid-tight membrane close to the inflow end of the mobile phase, the outer diameter of the polymer liquid-tight membrane is smaller than the inner diameter of the column tube, the central axis of the polymer liquid-tight membrane coincides with the central axis of the column tube, and the polymer liquid-tight membrane is parallel to the cross section of the column tube. The extraction column can solve the problem that the extraction column is easy to block, is easy to clean, and can prolong the service life of the chromatographic column and improve the column efficiency.
Description
Technical Field
The invention relates to the field of liquid chromatographic columns, in particular to an extraction column.
Background
In solid phase extraction systems, on-line processing of samples is often performed using a chromatography column, typically an extraction column for sample processing. In two-dimensional liquid chromatography detection, extraction columns are mainly used for primary separation, purification and concentration of samples. In this process, the extraction column is subject to contamination from various impurities in the sample, which tend to accumulate at the inlet end, resulting in high pressure, which severely affects the useful life of the extraction column.
The mobile phase flows through the chromatographic column, and because a large amount of impurities exist in the sample, particularly the protein in the biological sample is subjected to a mass aggregation phenomenon after being intercepted by the sieve plate, the degree of sieve plate blockage is more and more serious as the sampling frequency of the chromatographic column is more and the time is longer, which is also the most common problem of the modern liquid chromatographic column. Extraction columns are typically subjected to a larger sample volume than conventional chromatography columns, and so the columns are more susceptible to plugging.
The existing solution is to perform ultrasonic cleaning on the extraction column or replace column head filler to repair the column head, but the existing solution needs high specialization and has limited capability of solving the problem.
The applicant has obtained a patent for a liquid chromatograph with on-line cleaning function, with the following publication numbers: CN104330502B, mainly aim at the online washing of two-dimensional liquid chromatogram's first chromatographic column and middle chromatographic column, have the back washing function. Of course, this is only one specific chromatographic system. The chromatographic column can be cleaned on line or off line in a reverse cleaning mode.
In addition, in the conventional on-line extraction column, after a sample enters a stationary phase along with a mobile phase, a target object can make regular motion at the early stage and enters a detector for analysis, and a relatively normal chromatogram can be obtained. With the continuous accumulation of protein and other impurities in the sample in the stationary phase, part of the stationary phase loses the adsorption capacity, and the target object starts to make various irregular motions in the stationary phase and enters a detector for analysis to obtain a particularly trailing chromatogram.
Therefore, it is important to develop a novel extraction column for liquid chromatography to solve the above problems.
Disclosure of Invention
The invention aims to provide an extraction column, which can solve the problem that the extraction column is easy to block, is easy to clean, prolongs the service life of a chromatographic column, improves the column efficiency and keeps a better peak shape for a long time.
(II) in order to realize the purpose of the invention, the technical scheme of the invention is as follows:
an extraction column for liquid chromatography takes the inflow end of a mobile phase as the upper end, and comprises a column cap, a front-end column cap, a column tube, a tail-end column cap and a column cap which are sequentially connected from top to bottom, wherein a stationary phase is filled in the column tube, the upper end of the front-end column cap is provided with a pressure-variable pore structure, the pressure-variable pore structure comprises an upper layer, a middle layer and a lower layer, and an elastic soft plate, a high polymer filter membrane and a support pore plate are sequentially arranged from top to bottom; the stationary phase is internally provided with a polymer liquid-tight membrane close to the inflow end of the mobile phase, the outer diameter of the polymer liquid-tight membrane is smaller than the inner diameter of the column tube, the central axis of the polymer liquid-tight membrane coincides with the central axis of the column tube, and the polymer liquid-tight membrane is arranged parallel to the cross section of the column tube.
Preferably, the flexible soft plate comprises a fixed ring and a flexible sieve plate positioned in the fixed ring.
Preferably, the material of the fixing ring is preferably PEEK ring, the material of the fixing ring is polyether-ether-ketone, and the material of the PE sieve plate is preferably polyethylene. The material has the characteristics of high strength, low dissolution and the like, and the thickness of the PE sieve plate is between 0.5mm and 8 mm.
Preferably, the thickness of the elastic soft plate is 0.5mm-8mm, and the pore diameter is 5 microns-10 microns.
Preferably, the thickness of the polymeric filter membrane is 0.1 mm-0.5 mm, and the material with molecular weight larger than 5000Da can be intercepted.
Preferably, the material of the polymeric filter membrane is a material which can resist organic and acid-base reagents, and can be polyether sulfone resin or polypropylene.
Preferably, the material of the polymer liquid-tight membrane can be any liquid-tight membrane, and can be a fluorine-containing polymer material, preferably a polyester film or a nylon film.
Preferably, the gap between the periphery of the polymer liquid-tight membrane and the inner wall of the column tube is smaller than 1/4 of the inner diameter of the column tube, and the control is reasonable in the range. Further preferably, the gap between the outer periphery of the polymer liquid-impermeable film and the inner wall of the column tube is 1 to 3mm, and according to a great deal of research by the inventors, the rectification effect is better when the gap is 1 to 3 mm.
Preferably, the metal sieve plate is arranged above the polymer liquid-tight membrane and used for supporting the polymer liquid-tight membrane and preventing the polymer liquid-tight membrane from deforming.
Preferably, the structure of the supporting orifice plate may be identical to that of the flexible plate.
Preferably, the column head is provided with a hole for flowing in or flowing out the mobile phase.
Preferably, the inlet end of the column tube is provided with a front-end column cap, the outlet end of the column tube is provided with a tail-end column cap, the inlet end of the column tube is arranged in a column head, internal threads are arranged in the column head, the outlet end of the column tube is arranged in the column head, the internal threads are arranged in the column head, and the column tube is connected with the column head through the threads.
(III) the principle of the invention is illustrated:
1. description of the principle of easy cleaning of extraction columns with pressure-swing pores to improve the lifetime of the extraction column:
the pressure-variable pore soft plate and the polymer filter membrane are arranged in the column head at the inlet end, the background pressure of the extraction column corresponding to the one-dimensional mobile phase is generally about 2MPa and is not enough to form high pressure, when the mobile phase enters at a high flow rate, the high pressure is formed and generally reaches more than 10MPa, the elastic soft plate shrinks downwards, the pore diameter is reduced, filter particles are intercepted, more fine impurities are intercepted and filtered by the polymer membrane, and the support pore plate supports the elastic soft plate and the polymer membrane; during on-line and off-line reverse cleaning, the pressure is reduced, the elastic soft plate is deformed and expanded, and impurities intercepted by the organic polymer filter membrane and the elastic soft plate are reversely eluted and discharged. The utility model provides an extraction column with press and become hole, can be easier go out extraction column entry end impurity reverse cleaning to promote extraction column's life.
2. The principle that the polymer liquid-impermeable film can shape the mobile phase so as to improve the column efficiency is explained as follows:
the invention relates to a normal chromatographic column, wherein a sample flows through a stationary phase along with a mobile phase, the stationary phase loses adsorption capacity along with the accumulation of impurities such as protein in the sample, so that a target object in the sample does various irregular motions, the flow velocity nearest to the center of the chromatographic column is the maximum, the flow velocity near the periphery of the chromatographic column is small, and the flow velocity is uneven.
Compared with the prior art, the invention has the advantages that:
1. the extraction column provided by the invention has a pressure-variable gap structure, and impurities at the inlet end of the extraction column can be more easily cleaned out in a reverse direction, so that the service life of the extraction column is prolonged to more than 1000 needles.
2. The extraction column with the rectification characteristic rectifies the flow shape and the flow velocity of fluid entering the chromatographic column, the flow velocity becomes uniform and the flow direction becomes regular, so that target objects in a sample are rearranged after rectification and move forward in a synchronous manner, the peak shape of the extraction column is obviously improved, a relatively normal peak shape can be maintained, and more than 1000 needles can be maintained.
The detailed structure of the present invention will be further described with reference to the accompanying drawings and the detailed description.
Drawings
FIG. 1 is a schematic view of a split structure of an extraction column in example 1 of the present invention;
FIG. 2 is a schematic sectional view of an extraction column according to example 1 of the present invention;
FIG. 3 is a schematic top view of the flexible printed circuit board according to the present invention;
FIG. 4 is a schematic view of the present invention in example 1 with a metal sieve plate added;
FIG. 5 is a graph comparing the peak shape change of example 1 of the present invention with that of comparative example 1;
FIG. 6 is a graph of a 1000 pin peak contrast test for example 1 of the present invention;
FIG. 7 is a comparison graph of the spike shape of comparative example 1 test 200 of the present invention;
FIG. 8 is a graph comparing the pressure change of example 1 of the present invention with that of comparative example 1;
wherein, 1 is column cap, 2 is macromolecule filter membrane, 3 is elastic soft board, 4 is front end column cap, 5 is column tube, 6 is end column cap, 7 is fixed phase, 8 is supporting pore plate, 9 is fixed ring, 10 is sieve plate, 11 is macromolecule liquid-tight membrane, 12 is metal sieve plate.
Detailed Description
Example 1: as shown in fig. 1-4, an extraction column, using the inflow end of the mobile phase as the upper end, from the top to the bottom, the extraction column comprises a column cap 1, a front end column cap 4, a column tube 5, a terminal column cap 6 and a column cap 1, which are connected in sequence, the front end of the front end column cap 4 is provided with an elastic soft plate 3, a polymeric filter membrane 2 and a support pore plate 8 from the top to the bottom in sequence (the elastic soft plate 3, the polymeric filter membrane 2 and the support pore plate 8 form a new pressure-variable pore structure). The column head 1 is internally provided with a hole for flowing in or flowing out the mobile phase. The column tube 5 is internally filled with a stationary phase 7. The inlet end of the column tube 5 is provided with a front-end column cap 4, the outlet end of the column tube 5 is provided with a tail-end column cap 6, the inlet end of the column tube is arranged in the column cap 1, internal threads are arranged in the column cap 1, the outlet end of the column tube 5 is arranged in the column cap 1, internal threads are arranged in the column cap 1, and the column tube 5 is in threaded connection with the column cap 1. A polymer liquid-tight membrane 11 is arranged in the stationary phase 7 near the inflow end of the mobile phase, and the outer diameter of the polymer liquid-tight membrane 11 is smaller than the inner diameter of the column tube 5. The central axis of the polymer liquid-tight membrane 11 is superposed with the central axis of the column tube 5. The gap between the outer periphery of the polymer liquid-impermeable film 11 and the inner wall of the column tube 5 is smaller than 1/4 of the inner diameter of the column tube 5, which is 1mm in example 1.
As shown in fig. 3, the flexible elastic plate 3 is composed of a PEEK ring and a PE sieve plate, the PEEK ring is made of polyetheretherketone, and the PE sieve plate is made of polyethylene. The material has the characteristics of high strength, easy dissolution and the like.
The material of the polymeric filter membrane 2 is polyether sulfone (PES) or polypropylene (PP), and the material has high strength, chemical resistance and thermal stability.
The support pore plate 8 can be a support mesh plate, or the support pore plate 8 is composed of a PEEK ring and a PE sieve plate, the PEEK ring is made of PEEK, and the PE sieve plate is made of polyethylene. The material has the characteristics of high strength, easy dissolution and the like.
The column tube 5 and the column cap 1 are both made of stainless steel.
The thickness of the elastic soft plate 3 is 0.5mm, the aperture is 5 microns, the thickness of the macromolecular filter membrane 2 is 0.1mm, and substances with molecular weight larger than 5000Da can be intercepted.
The top of the polymer liquid-tight membrane is provided with a metal sieve plate for supporting the polymer liquid-tight membrane and preventing the polymer liquid-tight membrane from deforming.
Description of the working principle: after being subjected to protein precipitation treatment, a blood sample with a complex matrix enters an extraction column along with a liquid chromatography mobile phase, impurities with large molecular weight in the sample are intercepted when passing through an elastic soft plate, substances with medium and small molecular weights pass through an elastic filter plate and then are intercepted and filtered for the second time when passing through an organic polymer filter membrane, finally, a measured object with small molecular weight and part of impurities in the sample enter an extraction column stationary phase through a second-stage elastic soft plate and an anti-blocking filter cap, the measured object is primarily separated from the sample under the chromatographic action force of the extraction column immobility, and the separated object flows into a pipeline through an outlet filter cap and enters other separation systems or detection systems. After the extraction column with the pressure-variable pore structure finishes complex sample treatment for multiple times or once, offline or online, the flow with certain elution strength can be used in reverse phase to perform reverse phase cleaning and purification on the extraction column, and impurities intercepted by the organic polymer filter membrane and the elastic soft plate can deform in the expansion and contraction elasticity of the elastic soft plate, so that the impurities are promoted to be discharged, and the self-purification of the extraction column is finished. Meanwhile, the invention shapes the fluid by adding a polymer liquid-impermeable film, the flow direction track of the fluid is changed after the fluid meets the polymer liquid-impermeable film, the fluid uniformly flows out from the periphery of the polymer liquid-impermeable film from the longitudinal cross line, the flow speed is uniform, the flow direction is regular, the sample is more uniformly dispersed after rectification, the separation of the sample is more facilitated, the column efficiency is improved, and the peak shape of the extraction column is better.
The following percentages are volume percentages, and the extraction column in example 1 and a conventional extraction column were used to determine carbamazepine in human blood. Blood samples were treated with 67% acetonitrile +33% ethanol. The test is carried out on a liquid chromatograph with an on-line cleaning function, and the mobile phase is 30% acetonitrile and 70% water. The flow rate is set to be 1.0mL/min, the auxiliary mobile phase is 0.5% formic acid solution, the auxiliary flow rate is 2.0mL/min, the auxiliary time duration is 1.0min, the wavelength is 282nmnm, the sample injection volume is 50 muL, and the column temperature is 40 ℃. Test 1000 needles, look for peak-type changes (see fig. 5).
Valproic acid in a human blood sample is extracted by using the extraction column in the example 1 and a conventional extraction column on a liquid chromatograph with an online cleaning function, the mobile phase is 30% acetonitrile and 70% water, the set flow rate is 1.0mL/min, the sample injection is tested for 1000 needles, and the pressure change is checked (see figure 6).
According to the test results, as shown in fig. 7 and fig. 8, when the conventional extraction column extracts a sample, the pressure is continuously increased, the pressure reaches 18MPa at 400 needles, and the tolerance pressure of the conventional liquid chromatography is generally 20 MPa. Meanwhile, when a sample is tested by the conventional extraction column, the peak shape is not shaped, so that the test sample is not focused and dispersed, and the peak collapse is serious when the sample is tested by 200 needles. The extraction column of the embodiment 1 has the advantages that the inlet end is additionally provided with the pressure-variable pore structure, molecules can be intercepted in time, impurities and protein attached to the inlet end can be washed out in time through reverse cleaning, and the pressure is not obviously increased. Meanwhile, the newly added molecular interception membrane can reshape the target substance on the stationary phase again without diffusion, and the peak shape is kept normal and can be maintained for more than 1000 needles.
The above description is for the purpose of illustrating the present invention and is not intended to limit the scope of the present invention, and any person skilled in the art can substitute or change the technical solution of the present invention and its conception within the technical scope of the present invention, and the technical solution and the concept of the present invention are also intended to be covered by the scope of the claims of the present invention.
Claims (10)
1. An extraction column for liquid chromatogram takes the inflow end of a mobile phase as the upper end, and comprises a column cap (1), a front end column cap (4), a column tube (5), a tail end column cap (6) and a column cap (1) which are sequentially connected from top to bottom, wherein a stationary phase (7) is filled in the column tube (5), and the extraction column is characterized in that the upper end of the front end column cap (4) is provided with a pressure-variable pore structure which comprises an upper layer, a middle layer and a lower layer, and an elastic soft plate (3), a polymer filter membrane (2) and a support pore plate (8) are sequentially arranged from top to bottom; the stationary phase (7) is internally provided with a polymer liquid-tight membrane (11) close to the inflow end of the mobile phase, the outer diameter of the polymer liquid-tight membrane (11) is smaller than the inner diameter of the column tube (5), the central axis of the polymer liquid-tight membrane (11) is coincided with the central axis of the column tube (5), and the polymer liquid-tight membrane (11) is arranged parallel to the cross section of the column tube (5).
2. Extraction column for liquid chromatography according to claim 1, characterized in that the flexible plate (3) comprises a fixed ring (9) and a flexible sieve plate (10) inside the fixed ring (9).
3. The extraction column for liquid chromatography as claimed in claim 2, wherein the thickness of the flexible plate (3) is 0.5mm to 8mm, the pore diameter is 5 microns to 10 microns, and the flexible sieve plate (10) is made of polyethylene or polypropylene.
4. The extraction column for liquid chromatography as claimed in claim 1, wherein the polymeric filtration membrane (2) has a thickness of 0.1mm to 0.5mm and a retention of substances with a molecular weight of > 5000 Da.
5. The extraction column for liquid chromatography according to any one of claims 1 to 4, wherein the polymeric filtration membrane (2) is made of polyethersulfone resin or polypropylene.
6. An extraction column for liquid chromatography according to any one of claims 1 to 4, wherein the clearance between the outer periphery of the polymer liquid-impermeable membrane (11) and the inner wall of the column tube (5) is less than 1/4 of the inner diameter of the column tube (5).
7. The extraction column for liquid chromatography according to any one of claims 1 to 4, wherein the gap between the outer periphery of the polymeric liquid-impermeable membrane (11) and the inner wall of the column tube (5) is 1 to 3 mm.
8. The extraction column according to any of claims 1 to 4, wherein the polymeric liquid impermeable membrane (11) is made of mylar or nylon.
9. An extraction column for liquid chromatography according to any one of claims 1 to 4, wherein a metal sieve plate (12) is provided above the polymer liquid-impermeable membrane (11).
10. The extraction column for liquid chromatography according to any of claims 1 to 4, wherein the column head (1) is provided with holes for the inflow or outflow of the mobile phase, the inlet end of the column tube (5) is provided with a front end column cap (4), the outlet end of the column tube (5) is provided with a rear end column cap (6), the inlet end of the column tube (5) is arranged in the column head (1), the column head (1) is internally provided with internal threads, the outlet end of the column tube (5) is arranged in the column head (1), the column head (1) is internally provided with internal threads, and the column tube (5) and the column head (1) are connected through threads.
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| CN202211002349.XA CN115078615B (en) | 2022-08-22 | 2022-08-22 | Extraction column for liquid chromatography |
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| CN202211002349.XA CN115078615B (en) | 2022-08-22 | 2022-08-22 | Extraction column for liquid chromatography |
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| CN115078615B CN115078615B (en) | 2022-12-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023155629A1 (en) * | 2022-08-22 | 2023-08-24 | 湖南德米特仪器有限公司 | Multi-mode matrix-resistant extraction column |
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