CN115074300A - Mixed microbial agent for inhibiting growth of false smut - Google Patents
Mixed microbial agent for inhibiting growth of false smut Download PDFInfo
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- CN115074300A CN115074300A CN202210932523.4A CN202210932523A CN115074300A CN 115074300 A CN115074300 A CN 115074300A CN 202210932523 A CN202210932523 A CN 202210932523A CN 115074300 A CN115074300 A CN 115074300A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 44
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 28
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 40
- 239000002994 raw material Substances 0.000 claims abstract description 39
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 28
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 28
- 239000000654 additive Substances 0.000 claims abstract description 26
- 230000000996 additive effect Effects 0.000 claims abstract description 26
- 239000003381 stabilizer Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 239000002270 dispersing agent Substances 0.000 claims abstract description 22
- 239000008213 purified water Substances 0.000 claims abstract description 22
- 241000186000 Bifidobacterium Species 0.000 claims abstract description 20
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 20
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 20
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000004472 Lysine Substances 0.000 claims abstract description 20
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims description 29
- 238000002360 preparation method Methods 0.000 claims description 16
- 238000005303 weighing Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 239000002054 inoculum Substances 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 5
- 229920002367 Polyisobutene Polymers 0.000 claims description 3
- CJZGTCYPCWQAJB-UHFFFAOYSA-L calcium stearate Chemical group [Ca+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O CJZGTCYPCWQAJB-UHFFFAOYSA-L 0.000 claims description 3
- 235000013539 calcium stearate Nutrition 0.000 claims description 3
- 239000008116 calcium stearate Substances 0.000 claims description 3
- 150000004985 diamines Chemical class 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 12
- 235000009566 rice Nutrition 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 4
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 230000006866 deterioration Effects 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 11
- 239000000203 mixture Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241001474928 Ustilaginoidea virens Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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Abstract
The invention discloses a mixed microbial agent for inhibiting growth of false smut, which relates to the technical field of agricultural production and comprises the following raw materials in percentage by mass: 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3 to 8 percent of dispersant, 3 to 8 percent of stabilizer and 3 to 8 percent of antioxidant. The microbial agent prepared by the invention has good effect on inhibiting the growth of false smut, improves the yield of rice, and simultaneously, 3-8% of stabilizing agent and 3-8% of antioxidant are added, so that the prepared microbial agent can be placed for a long time, and the condition of deterioration of the agent is avoided.
Description
Technical Field
The invention relates to the technical field of agricultural production, in particular to a mixed microbial agent for inhibiting the growth of false smut.
Background
Rice is one of the important food crops for human beings, has a long history of cultivation and eating, and is eaten by half of the population all over the world, mainly in Asia, the south of Europe, tropical America and parts of Africa. The total yield of rice accounts for the third crop in the world, is lower than that of corn and wheat, but can maintain the life of a large population, so that the united nations define 2004 as the international rice year.
In the process of planting rice, the rice is often affected by false smut, the yield of the rice is often influenced, the rice is often required to be sprayed with a medicament for ensuring the yield of the rice, the preparation process of the conventional medicament for preventing and treating the false smut is complicated, and meanwhile, the prevention and treatment effect on the false smut is poor, so that the rice has a space to be improved.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a mixed microbial agent for inhibiting the growth of false smut. The preparation method has the advantages that the prepared microbial agent has a good effect of inhibiting the growth of false smut, the yield of rice is improved, 3-8% of stabilizing agent and 3-8% of antioxidant are added, the prepared microbial agent can be placed for a long time, and the condition that the agent is deteriorated is avoided.
In order to achieve the purpose, the invention adopts the following technical scheme:
the mixed microbial agent for inhibiting the growth of false smut comprises the following raw materials in percentage by mass: 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3 to 8 percent of dispersant, 3 to 8 percent of stabilizer and 3 to 8 percent of antioxidant.
Preferably, the material composition comprises the following raw materials in percentage by mass: 10% of Siamese bacillus, 15% of lactobacillus plantarum, 15% of bifidobacterium, 10% of lysine bacillus, 40% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3% of dispersing agent, 3% of stabilizing agent and 4% of antioxidant.
Preferably, the material composition comprises the following raw materials in percentage by mass: 10% of Siamese bacillus, 10% of lactobacillus plantarum, 10% of bifidobacterium, 10% of lysine bacillus, 50% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3% of dispersing agent, 4% of stabilizing agent and 3% of antioxidant.
Preferably, the material composition comprises the following raw materials in percentage by mass: 10% of Siamese bacillus, 10% of lactobacillus plantarum, 10% of bifidobacterium, 15% of lysine bacillus, 40% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 5% of dispersing agent, 7% of stabilizing agent and 3% of antioxidant.
The preparation method of the mixed microbial agent for inhibiting the growth of false smut comprises the following steps:
the method comprises the following steps: weighing raw materials such as 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water, 3-8% of dispersing agent, 3-8% of stabilizing agent, 3-8% of antioxidant and the like by using a weighing device;
step two: then, pouring 10% -15% of Siamese bacillus, 10% -15% of lactobacillus plantarum, 10% -15% of bifidobacterium, 10% -15% of lysine bacillus and 40% -50% of purified water into a stirring tank, and stirring by using a stirring device;
step three: while stirring, adding 3-8% of dispersing agent, 3-8% of stabilizing agent and 3-8% of antioxidant while stirring, and stirring for 10-20 minutes after adding until each raw material is fully mixed and dissolved in purified water;
step four: and after the raw materials are fully fused, the microbial agent can be prepared, and finally the microbial agent can be filled in a medicine by using a filling device for sale, so that the preparation of the microbial agent is completed.
The invention is further configured in that the preparation method is carried out by a preparation system, and the preparation system comprises a weighing device, a stirring device and a filling device.
The invention further provides that the weighing device is at least one of a balance, an electronic platform scale and an electronic weight-hanging scale, and is preferably the electronic platform scale. The electronic platform scale can display the quality of the weighed object in real time, and the convenience of operation is improved.
The stirring device further comprises a handheld shell, the inner wall of the handheld shell is provided with a motor, an output shaft of the motor is connected with a rotating rod, and the outer wall of the rotating rod is provided with a plurality of stirring rods which are distributed at equal intervals. During the stirring, the handheld shell of workman stretches into the puddler to the agitator tank in, and control motor work drives the dwang and rotates, and the dwang drives the puddler and rotates and stir the mixture to the raw materials, has improved the production efficiency of microbial inoculant.
The invention is further configured such that the filling device is at least one of a fully automatic filling machine, a gravity-type negative pressure filling machine and a rotary liquid dosing filling machine, and preferably a rotary liquid dosing filling machine. The rotary liquid quantitative filling machine can conveniently realize the quantitative filling of the microbial agent, and the convenience of the filling operation is improved.
The invention is further configured such that the stabilizer is calcium stearate, the dispersant is polyisobutylene diamine, and the antioxidant is a water-soluble antioxidant.
The beneficial effects of the invention are as follows: according to the mixed microbial agent for inhibiting the growth of the false smut, 10% -15% of Siamese bacillus, 10% -15% of lactobacillus plantarum, 10% -15% of bifidobacterium and 10% -15% of lysine bacillus are used as main raw materials to be fully mixed, the prepared microbial agent has a good effect of inhibiting the growth of the false smut, the yield of rice is improved, 3% -8% of a stabilizing agent and 3% -8% of an antioxidant are added, the prepared microbial agent can be guaranteed to be placed for a long time, and the condition that the agent is deteriorated is avoided.
Drawings
FIG. 1 is a schematic view of the raw material composition structure of the mixed microbial agent for inhibiting the growth of false smut according to the present invention;
FIG. 2 is a schematic view of the preparation process of the mixed microbial agent for inhibiting the growth of ustilaginoidea virens according to the present invention.
Detailed Description
The technical solution of the present patent will be further described in detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Example 1
Referring to fig. 1, the mixed microbial agent for inhibiting the growth of false smut comprises the following raw materials in percentage by mass: 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3 to 8 percent of dispersant, 3 to 8 percent of stabilizer and 3 to 8 percent of antioxidant.
Example 2
Referring to fig. 1, the mixed microbial agent for inhibiting the growth of false smut comprises the following raw materials in percentage by mass: 10% of Siamese bacillus, 15% of lactobacillus plantarum, 15% of bifidobacterium, 10% of lysine bacillus, 40% of purified water and an auxiliary additive, wherein the auxiliary additive comprises the following raw materials in percentage by mass: 3% of dispersing agent, 3% of stabilizing agent and 4% of antioxidant.
Example 3
Referring to fig. 1, the mixed microbial agent for inhibiting the growth of false smut comprises the following raw materials in percentage by mass: 10% of Siamese bacillus, 10% of lactobacillus plantarum, 10% of bifidobacterium, 10% of lysine bacillus, 50% of purified water and an auxiliary additive, wherein the auxiliary additive comprises the following raw materials in percentage by mass: 3% of dispersing agent, 4% of stabilizing agent and 3% of antioxidant.
Example 4
Referring to fig. 1, the mixed microbial agent for inhibiting the growth of false smut comprises the following raw materials in percentage by mass: 10% of Siamese bacillus, 10% of lactobacillus plantarum, 10% of bifidobacterium, 15% of lysine bacillus, 40% of purified water and an auxiliary additive, wherein the auxiliary additive comprises the following raw materials in percentage by mass: 5% of dispersing agent, 7% of stabilizing agent and 3% of antioxidant.
Referring to fig. 2, the method for preparing the mixed microbial agent for inhibiting the growth of false smut comprises the following steps:
the method comprises the following steps: weighing raw materials such as 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water, 3-8% of dispersing agent, 3-8% of stabilizing agent, 3-8% of antioxidant and the like by using a weighing device;
step two: then, pouring 10% -15% of Siamese bacillus, 10% -15% of lactobacillus plantarum, 10% -15% of bifidobacterium, 10% -15% of lysine bacillus and 40% -50% of purified water into a stirring tank, and stirring by using a stirring device;
step three: while stirring, adding 3-8% of dispersing agent, 3-8% of stabilizing agent and 3-8% of antioxidant while stirring, and stirring for 10-20 minutes after adding until each raw material is fully mixed and dissolved in purified water;
step four: and after the raw materials are fully fused, preparing the microbial agent, and finally filling the microbial agent into a medicine by using a filling device for sale, thereby completing the preparation of the microbial agent.
In this embodiment, the preparation method is implemented by a preparation system, and the preparation system includes a weighing device, a stirring device, and a filling device.
In this embodiment, weighing device is balance, electronic platform balance and the electron hangs at least one in the platform balance, and preferably electronic platform balance, but electronic platform balance real-time display is weighed the quality of article, has improved the convenience of operation.
In this embodiment, agitating unit includes handheld shell, and the inner wall of handheld shell is provided with the motor, and the output shaft of motor has the dwang, and the outer wall of dwang is provided with the puddler that a plurality of equidistance distribute, and during the stirring, the handheld shell of workman stretches into the puddler to the agitator tank in, and control motor work drives the dwang and rotates, and the dwang drives the puddler and rotates and stir the mixture to the raw materials, has improved the production efficiency of microbial inoculant.
In this embodiment, the filling device is at least one of a full-automatic filling machine, a gravity type negative pressure filling machine and a rotary liquid quantitative filling machine, and is preferably a rotary liquid quantitative filling machine, and the rotary liquid quantitative filling machine can conveniently realize quantitative filling of the microbial agent, so that convenience of filling operation is improved.
In this example, the stabilizer was calcium stearate, the dispersant was polyisobutylene diamine, and the antioxidant was a water soluble antioxidant.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (10)
1. The mixed microbial agent for inhibiting the growth of false smut is characterized by comprising the following raw materials in percentage by mass: 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3 to 8 percent of dispersant, 3 to 8 percent of stabilizer and 3 to 8 percent of antioxidant.
2. The mixed microbial agent for inhibiting the growth of false smut according to claim 1, comprising the following raw materials in percentage by mass: 10% of Siamese bacillus, 15% of lactobacillus plantarum, 15% of bifidobacterium, 10% of lysine bacillus, 40% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 3% of a dispersant, 3% of a stabilizer and 4% of an antioxidant.
3. The mixed microbial agent for inhibiting the growth of false smut according to claim 1, comprising the following raw materials in percentage by mass: 10% of Siamese bacillus, 10% of lactobacillus plantarum, 10% of bifidobacteria, 10% of lysine bacillus, 50% of purified water and an auxiliary additive, wherein the auxiliary additive comprises the following raw materials in percentage by mass: 3% of dispersing agent, 4% of stabilizing agent and 3% of antioxidant.
4. The mixed microbial agent for inhibiting the growth of false smut according to claim 1, comprising the following raw materials in percentage by mass: 10% of Siamese bacillus, 10% of lactobacillus plantarum, 10% of bifidobacterium, 15% of lysine bacillus, 40% of purified water and an auxiliary additive, wherein the auxiliary additive is prepared from the following raw materials in percentage by mass: 5% of dispersing agent, 7% of stabilizing agent and 3% of antioxidant.
5. A method for preparing the mixed microbial agent for inhibiting the growth of false smut according to claim 1, comprising the steps of:
the method comprises the following steps: weighing raw materials such as 10-15% of Siamese bacillus, 10-15% of lactobacillus plantarum, 10-15% of bifidobacterium, 10-15% of lysine bacillus, 40-50% of purified water, 3-8% of dispersing agent, 3-8% of stabilizing agent, 3-8% of antioxidant and the like by using a weighing device;
step two: then, pouring 10% -15% of Siamese bacillus, 10% -15% of lactobacillus plantarum, 10% -15% of bifidobacterium, 10% -15% of lysine bacillus and 40% -50% of purified water into a stirring tank, and stirring by using a stirring device;
step three: while stirring, adding 3-8% of dispersing agent, 3-8% of stabilizing agent and 3-8% of antioxidant while stirring, and stirring for 10-20 minutes after adding until each raw material is fully mixed and dissolved in purified water;
step four: and after the raw materials are fully fused, the microbial agent can be prepared, and finally the microbial agent can be filled in a medicine by using a filling device for sale, so that the preparation of the microbial agent is completed.
6. The method for preparing a mixed microbial inoculant for inhibiting the growth of false smut according to claim 5, wherein the preparation method is implemented by a preparation system, and the preparation system comprises a weighing device, a stirring device and a filling device.
7. The method for preparing a mixed microbial agent for inhibiting the growth of false smut according to claim 6, wherein the weighing device is at least one of a balance, an electronic platform scale and an electronic platform-hung scale, and is preferably an electronic platform scale.
8. The method for preparing a mixed microbial inoculant for inhibiting the growth of false smut according to claim 7, wherein the stirring device comprises a hand-held shell, the inner wall of the hand-held shell is provided with a motor, the output shaft of the motor is connected with a rotating rod, and the outer wall of the rotating rod is provided with a plurality of stirring rods which are distributed equidistantly.
9. The method for preparing a mixed microbial inoculant for inhibiting the growth of false smut according to claim 8, wherein the filling device is at least one of a full-automatic filling machine, a gravity type negative pressure filling machine and a rotary type liquid quantitative filling machine, and preferably a rotary type liquid quantitative filling machine.
10. The method for preparing a mixed microbial inoculant for inhibiting the growth of false smut according to claim 9, wherein the stabilizing agent is calcium stearate, the dispersing agent is polyisobutylene diamine, and the antioxidant is a water-soluble antioxidant.
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| CN116769677A (en) * | 2023-08-09 | 2023-09-19 | 宁夏三十六度生物科技有限公司 | Microbial agent for efficient growth of rice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116769677A (en) * | 2023-08-09 | 2023-09-19 | 宁夏三十六度生物科技有限公司 | Microbial agent for efficient growth of rice |
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