CN115058324A

CN115058324A - Device for realizing virus screening based on bowl-shaped centrifugal microfluidic chip

Info

Publication number: CN115058324A
Application number: CN202210841205.7A
Authority: CN
Inventors: 张佩刚
Original assignee: Shanghai Relax Material Technology Co ltd
Current assignee: Shanghai Relax Material Technology Co ltd
Priority date: 2022-07-18
Filing date: 2022-07-18
Publication date: 2022-09-16

Abstract

The invention relates to a device for screening viruses based on a bowl-shaped centrifugal microfluidic chip, wherein the device comprises: a clamping disc embedded with the cover disc through the base disc; the conical chip layer is matched with the clamping disc and is provided with a hollow cone with a microporous wall structure; the conical chip layer and the clamping disc are embedded to form a bowl-shaped chip which is arranged in a three-dimensional intersecting manner; and a motor shaft is inserted in the center of the clamping plate and used for realizing centrifugal mixing treatment on the bowl-shaped chip. By adopting the device for screening the viruses based on the bowl-shaped centrifugal microfluidic chip, the bowl-shaped chip is used without professional operators and aerosol pollution, only 3 minutes are needed for inputting trace samples, freeze-dried powder, reagents and color developing agents, and then the full-automatic operation and visual quick detection of multiple targets of the samples are realized by a centrifugal mixing and positive pressure driving mode, so that the detection accuracy and the screening speed can be obviously improved, and the device can be widely applied to occasions such as airport customs, community families and the like.

Description

Device for realizing virus screening based on bowl-shaped centrifugal microfluidic chip

Technical Field

The invention relates to the technical field of in-vitro diagnosis and micro-fluidic, in particular to a device for screening viruses based on a bowl-shaped centrifugal micro-fluidic chip, belonging to the technical field of biological detection.

Background

At present, the nasopharyngeal swab virus detection program has multiple operation steps, long time consumption, needs manual operation of professional experimenters, and has the defects of high false negative rate, aerosol pollution and leakage, and easy error in the operation process. How to popularize nucleic acid detection in global development and less developed areas faces a series of problems of how to overcome pollution, reduce cost, remove complex instruments, simplify operation and the like.

The existing solution provided by the nucleic acid detection microfluidic chip integrates a plurality of reaction steps into a microfluidic chip with a few square centimeters, and the microfluidic chip has the characteristics of controllable liquid flow, less consumption, quick analysis and the like, and has a huge development prospect as a new scientific technology. The miniaturization of a detection instrument, the simplification of sample pretreatment, the high efficiency and the accuracy of experiment operation and the economy of detection cost can be realized. At present, the detection technology based on PCR and a constant-temperature amplification method is mainly used, but the sampling of the method also depends on nasopharyngeal swabs with a lot of flaws and high false negative rate; in addition, most of the fluorescence detection needs professional detection equipment to interpret results, and due to the background fluorescence, the negative sample interpretation time is long; most of the chips only aim at single pathogen for detection, and cannot meet the requirement on flux; in addition, most microfluidic technologies require additional control equipment to maintain a stable pressure difference in the channel, which all challenge the requirements of POCT to be efficient, accurate, simple and fast.

The prior patent CN113652341a discloses a virus detector capable of "visual detection", although it is simple and convenient, has low professional requirement, and has very strong practicability. There are some disadvantages and inconveniences, such as: additional manual syringes and laboratory micro-syringe pumps were required, heating by water or metal bath (30 min at 65 ℃ constant temperature), 1 min time for cooling at room temperature was required [ after warming will affect the stability of viral nucleic acids, especially RNA, will affect the assay results ], and a separate centrifuge was required to mix the solutions. The multiple manual operations and additional time consumption thereof cannot be applied to mass production.

The existing virus screening has many difficulties and defects:

the research and development technology and the production technology are disjointed, so that the experimental technology of the micro-fluidic chip is more, but the mass production and application are less;

research and development technology is disjointed from market application, so that the virus kit has a plurality of patents, but is rarely used when being operated well and used by oneself;

sample collection is disjointed from virus extraction, so that the pharyngeal swab virus is greatly amplified by heating, but the high-virus sputum is rarely extracted directly;

the virus extraction and the nucleic acid detection are disjointed, so that a plurality of virus detection equipment and instruments are provided, and the virus detection equipment is fast, convenient, fast, convenient and free from people fatigue and is few;

in the face of continuous mutation of new coronavirus, a novel detection mode and a screening device which are frequent, low-cost, rapid and large-batch in detection, high in sensitivity and speed are required to be provided, and development of a screening tool which is simpler and more convenient and easier to popularize is urgent.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a device for screening viruses based on a bowl-shaped centrifugal microfluidic chip, which is used for carrying out multi-target detection on a single sample.

In order to achieve the above purpose, the device for screening viruses based on the bowl-shaped centrifugal microfluidic chip of the invention comprises the following components:

this device based on centrifugal micro-fluidic chip of bowl form realizes virus screening, its key feature is, the device include:

a clamping disc which is embedded with the cover disc through the base disc;

the conical chip layer is matched with the clamping disc and is provided with a hollow cone with a microporous wall structure;

the conical chip layer is embedded with the clamping disc to form a bowl-shaped chip which is arranged in a three-dimensional intersecting manner; and a motor shaft is inserted in the center of the clamping plate and used for realizing centrifugal mixing treatment on the bowl-shaped chip.

Preferably, the chassis is a flat-bottomed disc, a concave opening is formed in the upper end of the chassis, a central cylinder, a through shaft hole for inserting a motor and a positioning pin hole formed in the edge of the through shaft hole are sequentially arranged in the chassis;

the circumferential edge of the upper end of the chassis is provided with a chassis clamping groove with a bayonet, the bottom of the chassis is provided with an inwards concave bottom, and the positioning pin hole and the chassis clamping groove are used for positioning and embedding the disc cover of the clamping disc; and

the liquid inlet channel of the clamping plate is formed by combining a liquid inlet socket, a circular ring kit, a circular arc reaction tank, a circular pipe groove and a groove liquid guide pipe.

Preferably, the box body of the circular kit is made of plastic, and the annular outer vertical surface of the box body is provided with a structure with the upper end protruding outwards and the lower end inclining inwards, and is adhered and sealed by a film for preventing the reagent from splashing;

the bottom of the circular kit is of a structure which is high in center and inclined towards the circumferential direction, the circular kit is provided with N independent arc sections according to the angle, each arc section is divided into M fan-shaped grids of independent fan grids, more than one powder or reagent and a channel for inputting gas are pre-buried in each fan-shaped grid, one end of the gas channel is communicated with the micro gas pump, and the other end of the gas channel is communicated with the circular pipe groove and the groove through hole of the groove liquid guide pipe.

Preferably, the arc reaction tank is uniformly provided with a plurality of arc cavities according to a proportion to contain reagents and reaction liquid, and at least more than one virus screening primer is pre-buried in each arc cavity and is used for detecting pathogens;

the circular tube grooves are uniformly divided and arranged at the periphery of each section of the circular arc cavity according to the same proportion as the circular arc reaction tank, and the bottom of each section of the circular arc cavity is provided with the groove liquid guide pipe; and a reverse wedge ring slot is also arranged below the groove liquid guide pipe.

Preferably, the circular ring kit is further provided with two box bodies distributed inside and outside, wherein the inner layer is provided with a small circular ring kit with a reduced single structure, a circular ring liquid storage tank is arranged on the periphery of the small circular ring kit and used for increasing the addition of reagents, primers and probes are uniformly arranged on the periphery of the circular ring liquid storage tank, and the primers and the probes are embedded in the circular arc cavities at corresponding positions.

Preferably, the disk cover is a disk with an axial hole, and an outer hexagonal reverse-tooth nut and a positioning pin arranged at the edge of the axial hole are arranged at the axial hole of the disk cover;

a circular hole ring, a liquid inlet socket, a disc cover spigot and a disc cover buckle are sequentially arranged outwards along the shaft hole, and the outer hexagonal inverse-tooth nut, the positioning pin, the disc cover spigot and the disc cover buckle are used for being jointly used for accurately embedding and fixing the disc cover on the chassis of the clamping disc; the round hole circle be arranged in inserting a plurality of shower nozzles in order to input several kinds of reagent respectively to input malleation gas in the follow air pump, feed liquor socket be arranged in inserting the feed liquor funnel in order to carry out input sample liquid.

Preferably, the liquid inlet funnel is provided with an oval open upper part, a funnel-shaped middle part and a conical through pipe at the outlet end of the lower part, and is used for collecting oral sputum samples, the open opening of the liquid inlet funnel is provided with an oval reagent bag plug capable of being plugged into an extrusion type plastic reagent bag, the edge of the inner side of the open opening of the liquid inlet funnel is provided with a needling component, and a fiber filter layer is padded in the middle of the liquid inlet funnel; two sides of a conical pipe at the lower part of the liquid inlet funnel are provided with ribbed membrane strips which correspond to the liquid inlet sockets in size and are fixedly arranged at the upper part of the conical pipe, and the lower part of each ribbed membrane strip is provided with a movable strip block for locking and stopping the left side and the right side of the liquid inlet funnel; the reagent pack plug is internally provided with virus lysate, and the needling component is used for inserting and puncturing the extrusion type plastic reagent pack, so that the virus lysate is filled to wash viscous sputum filtered and blocked by the fiber filtering layer.

Preferably, the conical chip layer is provided with a thermoplastic resin material with the same size as the conical chip layer as a chip base layer, a layer of PET coiled material is pasted outside the chip base layer as a chip cover layer of the conical chip layer, and a layer of PE coiled material is pasted outside the chip cover layer as a chip protection layer of the conical chip layer;

the conical chip layer is also provided with a single-side chip layer or double-side chip layers; the single-side chip layer is provided with at least four groups of screening channels only on the outer surface of the chip base layer; the double-side chip layer is characterized in that more than four groups of screening channels are respectively arranged on the inner side surface and the outer side surface of the chip base layer.

Preferably, the chip cover layer is manufactured with a plurality of large hole ends and small hole ends with preset sizes through slitting, edge pressing and curling, each large hole end and each small hole end are respectively arranged at the upper closed circular ring end and the lower closed circular ring end of the conical chip layer, and each large hole end and each small hole end are set to be in a closed state;

at least more than four liquid inlet pipes coated with hot melt quick drying glue layers are equidistantly arranged on the circumference of the upper closed circular ring end of the small hole end; each section of the conical chip layer is also provided with a group of notches coated with hot-melt quick-drying adhesive along the height direction of the conical chip layer, and the conical chip layer of each section is formed by crimping after the notches are butted and bonded;

the small hole end of the conical chip layer is inserted into the inverted wedge ring slot, the groove liquid guide pipe is inserted into the corresponding liquid inlet pipe, the end face of the small hole end of the conical chip layer is coated with hot melt adhesive, and the hot melt adhesive is used for bonding the inverted wedge ring slot arranged on the bottom of the chassis so as to realize matching assembly of the bowl-shaped chip.

Preferably, the conical chip layer is provided with a preset number of screening channels which are arranged vertically along the conical chip layer or in a parabola shape along the rotation direction of the conical chip layer, each screening channel is respectively provided with a liquid inlet pipe, at least more than one flow splitting channel, a backflow prevention buckle, a pre-assembly port, an upper blocking channel, a lower blocking channel, a square color developing groove and a waste liquid groove, the diameters of inlets of the upper blocking channel and the lower blocking channel are respectively set to be larger than the diameter of the square color developing groove, the upper blocking channel and the lower blocking channel are communicated with the square color developing groove, the waste liquid groove is arranged on two sides of the square color developing groove, water absorption paper is further arranged between the square color developing groove and the waste liquid groove, an exhaust groove is arranged above the waste liquid groove, and the waste liquid groove is communicated with the exhaust groove through an overflow prevention pipeline, the exhaust groove is also provided with an exhaust hole, and the exhaust hole is used for exhausting gas in a pipeline of the bowl-shaped chip in the processes of inputting, vibrating, centrifuging and positive pressure of a liquid sample;

and the waste liquid groove is pre-embedded with a high polymer water absorption material, the upper blocking channel and the lower blocking channel are provided with microspheres which are filled in the pre-assembly port, the microspheres are used for preventing the reagent from being blocked, the bottoms of the upper blocking channel and the lower blocking channel are provided with anti-falling buckles, and the anti-falling buckles are used for preventing the microspheres from falling.

This automation equipment based on centrifugal micro-fluidic chip of bowl form realizes virus screening, its key feature is, automation equipment contain the above bowl form chip, press from both sides solid dish, circular cone chip layer, chassis and cover plate, wherein, the bowl form chip adopt the bull peristaltic pump to follow the top of bowl form chip annotate the liquid and handle, just automation equipment still be provided with feed liquor funnel, aerosol sample thief, medium temperature sterilization case and screening window baffle.

The desktop device for realizing virus screening based on the bowl-shaped centrifugal microfluidic chip is mainly characterized by comprising the bowl-shaped chip, a clamping disc, a conical chip layer, a base plate and a cover disc, wherein the bowl-shaped chip is extracted from a bottom box by adopting an intelligent vacuum pump and a water pump and is injected from the top of the bowl-shaped chip, the bowl-shaped chip is formed by superposing a cylindrical outer sleeve, an inner sleeve and a disc-shaped bottom box, a color sensor is arranged on the inner wall of the outer sleeve, an arc-shaped screen is attached to the arc of the outer wall of the outer sleeve, and arc-shaped nozzles and outer sleeves with preset numbers are arranged in the top of the outer sleeve; the miniature motor is arranged at the center of the top of the inner sleeve, the miniature motor shaft is arranged in the direction which is vertically and upwards protruded from the miniature motor, a positioning turntable is arranged above the miniature motor shaft, the positioning turntable comprises a rotating disc, a fixed disc arranged at the bottom of the rotating disc and a positioning steel ball arranged on the fixed disc, and the rotating disc is provided with the bowl-shaped chip.

Preferably, the bottom case comprises a bottom cover and a base, a large inserting buckle and a small inserting buckle are arranged on the bottom cover, the large inserting buckle is used for inserting and fixing the outer sleeve, and the small inserting buckle is used for inserting and fixing the inner sleeve; the central position of the bottom cover is provided with an intelligent vacuum water pump, a micro water pump A, a micro water pump B and a control electric plate; the bottom cover is arranged on the base, the base is also provided with a foot seat, and a storage area A, a storage area B and a storage area C for storing liquid reagents are arranged in the base in a divided mode; intelligence vacuum pump, miniature pump first, miniature pump second through the straw with storage area first, storage area second and storage area third be connected to adopt plastic pipe and each circular-arc nozzle be connected, the control electroplax with intelligence vacuum pump, miniature pump first, miniature pump second, color sensor, circular-arc screen and miniature motor be connected mutually the electricity.

This wearable device based on centrifugal micro-fluidic chip of bowl form realizes virus screening, its key feature is, the device contain the above-mentioned bowl form chip, press from both sides solid dish, circular cone chip layer, chassis and cover plate, wherein, the bowl form chip for adopt divide into the interval of predetermineeing the number in the ring kit, carry out the screening reagent that pours into in advance to positive pressure mode through manual pressure-on reagent passageway and blow through the mouth sets up, and by wearable device operation switch button selection function switch, display screen and corresponding demonstration and electroplax, realize the screening through with wearable device cover press carry out the mode of perpendicular operation on vertical cylindric wearable base.

The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip is used as a noninvasive, efficient, day and night attended airport customs and a home direct sputum collection and detection mode, and multiple reactions of a conventional analysis and detection method are integrated on the bowl-shaped centrifugal microfluidic chip. The kit can be widely applied to detection of various respiratory viruses, can obviously improve the accuracy and the detection speed of detection results, and can screen patients with no symptoms or at the early stage of disease, thereby avoiding the risk of cross infection. Meanwhile, the device is suitable for operation and carrying, simple, convenient, efficient, economical and durable in production and manufacture, and is very suitable for large-scale popularization and application.

Drawings

Fig. 1 is a schematic front view of a bowl-shaped chip of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 2 is a schematic top view of a bowl-shaped centrifugal microfluidic chip-based device for screening viruses according to a first embodiment of the present invention.

Fig. 3 is a schematic front view of a base plate of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 4 is a schematic top view of a bottom plate of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 5 is a schematic front view of a kit of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 6 is a schematic top view of a kit for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip according to a first embodiment of the present invention.

Fig. 7 is a schematic front view of a cover plate of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 8 is a schematic top view of a cover plate of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 9 is a schematic front view of a clamping plate of a first embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 10 is a schematic front view of a chip layer of a device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to a first embodiment of the present invention.

Fig. 11 is a schematic top view of a chip layer of a device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to a first embodiment of the present invention.

Fig. 12 is a schematic diagram of chip layer bonding of a first embodiment of the device for realizing virus screening based on a bowl-shaped centrifugal microfluidic chip.

Fig. 13 is a schematic diagram of a first embodiment of a liquid inlet funnel of the device for realizing virus screening based on the bowl-shaped centrifugal microfluidic chip.

Fig. 14 is a schematic front view of a clamping plate of a second embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip.

Fig. 15 is a schematic front view of a second embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 16 is a schematic top view of a second embodiment of the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 17 is a schematic diagram of an embodiment of an automated device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 18 is a schematic view of a window of an embodiment of the automated device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 19 is a schematic diagram of a desktop device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 20 is a schematic outer sleeve of a desktop device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 21 is a schematic diagram of an inner sleeve of the desktop device for screening viruses based on the bowl-shaped centrifugal microfluidic chip.

Fig. 22 is a schematic view of a rotating disc assembly of the desktop device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 23 is an exploded view of a rotary disk of the desktop device for screening viruses based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 24 is a schematic bottom cover view of the desktop-type device for screening viruses based on the bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 25 is a schematic view of a base of the desktop device for screening viruses based on the bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 26 is a schematic diagram of a wearable device for implementing virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 27 is a schematic diagram of the chip mounting of the wearable device for virus screening based on the bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 28 is a schematic diagram of a chip structure of the wearable device for realizing virus screening based on the bowl-shaped centrifugal microfluidic chip.

Fig. 29 is a schematic front view of a wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Fig. 30 is a schematic top view of a wearable device for virus screening based on a bowl-shaped centrifugal microfluidic chip according to the present invention.

Reference numerals

1, a bowl-shaped chip; 2, clamping and fixing the disc; 3 a conical chip layer; 4, a chassis; 5, covering the disc;

6, a liquid inlet channel; 7 screening the channel; 8, a concave opening; 9 a central cylinder; 10 is directly connected with a shaft hole;

11 positioning pin holes; 12 ring kit; 13 arc reaction tank; 14 circular tube grooves; 15 a grooved catheter;

16 chassis slots; 17, a concave bottom of the chassis; 18 a screening device; 19 a kit bevel; 20 bottom surface of the kit;

21, a circular cavity; 22 a fan-shaped grating; 23 arc cavity; 24 viral primers; 25, a circular tube cavity;

26 a reverse wedge ring slot; 27 outer hexagonal reverse-tooth nuts; 28 positioning pins; 29 circular hole rings; 30 liquid inlet sockets;

31, covering a spigot on the disc; 32 disc cover buckles; 33 an air pump; 34 a chip base layer; 35 a chip cap layer;

36 a chip protection layer; 37 liquid inlet pipe; 38 a flow-splitting channel; 39 anti-backflow buckle; 40 a pre-assembly port;

41 blocking the channel; 42 lower blocking passage; 43 microspheres; 44 anti-falling buckle; 45 square color development groove;

46 a waste liquid tank; 47 absorbent paper; 48 an overflow prevention duct; 49 exhaust grooves; 50 two-dimensional codes;

51 air exhaust holes; 52, a large-hole end; 53 small bore end; 54 hot melt adhesive; a 55 chip interface;

56 liquid inlet funnel; 57 a needling member; 58 a honeycomb sponge; 59 a ribbed membrane strip; 60 movable bars;

61 reagent pack stopper; 62 small circular kit; 63 a circular liquid storage tank; 64 primers and probes; 65 aerosol sampler;

66 negative pressure exhaust fan; 67 ultraviolet sterilizing lamps; 68 medium temperature sterilizing box; 69 screening window blinds; 70 disposable mask;

71 a fingerprint controller; 72 measuring the vibration handrail; 73, an antibacterial color-coated plate; 74 automatic heightening pad; 75 control the electric cabinet;

76 an automatic conveying table; 77 negative pressure exhaust fan; 78 circulating the conveying line; 79 chip fixing blocks; 80 micro air pump;

81 multi-head peristaltic pumps; 82 circular ring-shaped nozzles; 83 covering the funnel with a mask; 85 a desktop type device;

86 bottom box; 87 an outer sleeve; 88 a color sensor; 89 arc-shaped screens; a 90 arc-shaped nozzle;

91 an outer sleeve handle; 92 an inner sleeve; 93 a micro motor; 94 miniature motor shafts; 95 positioning rotary table;

95-1 rotating the disc; 95-2 fixing the disc; 95-3 positioning the steel balls; 96 bottom caps; 97 large plug-in buckle;

98 small plug-in fasteners; 99 intelligent vacuum water pump; 100 micro water pump A; 101, a miniature water pump B; 102 a control panel;

103 a base; 104 foot seats; 105 storage area a; 106 storage area B; 107 storage area C;

108 a wearable device; 109 switch keys; 110 display and electric board; 111 wearable base

Detailed Description

In order to more clearly describe the technical contents of the present invention, the following further description is given in conjunction with specific embodiments.

Before describing in detail embodiments that are in accordance with the present invention, it should be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Referring to fig. 1, the device for screening viruses based on a bowl-shaped centrifugal microfluidic chip includes:

a clamping disc 2 which is embedded with the cover disc 5 through the base disc 4;

a conical chip layer 3 which is matched with the clamping disc 2 and is provided with a hollow cone with a micro-pore wall structure;

the conical chip layer 3 is embedded with the clamping disc 2 to form a bowl-shaped chip 1 which is arranged in a three-dimensional intersecting manner; and a motor shaft is inserted in the center of the clamping plate 2 and used for realizing centrifugal mixing treatment on the bowl-shaped chip 1.

As a preferred embodiment of the present invention, the chassis 4 is a flat-bottom disc, a concave opening 8 is arranged at the upper end of the chassis 4, a central cylinder 9, a through shaft hole 10 for inserting a motor, and a positioning pin hole 11 arranged at the edge of the through shaft hole 10 are sequentially arranged in the chassis 4;

a chassis clamping groove 16 with a bayonet is arranged on the circumferential edge of the upper end of the chassis 4, an inward concave bottom is arranged at the bottom of the chassis 4, and the positioning pin hole 11 and the chassis clamping groove 16 are used for positioning and embedding the disc cover 5 of the clamping disc 2; and

the liquid inlet channel 6 of the clamping disc 2 is formed by combining a liquid inlet socket 30, a circular ring kit 12, a circular reaction tank 13, a circular pipe groove 14 and a groove liquid guide pipe 15.

As a preferred embodiment of the present invention, the box body of the annular reagent box 12 is made of plastic, and the annular outer vertical surface is configured as a structure with an upper end protruding outwards and a lower end inclining inwards, and is adhered and sealed by a film, so as to prevent the reagent from splashing;

the bottom of the circular kit 12 is set to be a structure with a high center and inclined towards the circumferential direction, the circular kit 12 is set to be provided with N independent arc sections according to an angle, each arc section is divided into M fan-shaped grids 27 with independent fan grids, more than one powder or reagent and a channel for inputting gas are pre-embedded in each fan-shaped grid 27, one end of the gas channel is communicated with the micro air pump, and the other end of the gas channel is communicated with the circular tube groove 14 and the groove through hole of the groove liquid guide tube 15.

As a preferred embodiment of the present invention, the arc reaction tank 13 is uniformly provided with a plurality of arc cavities 23 in proportion to accommodate reagents and reaction solutions, and each arc cavity 23 is pre-embedded with at least one screening viral primer 24 for detecting pathogens;

the circular tube grooves 14 are uniformly divided and arranged at the periphery of each section of the circular arc cavity 23 according to the same proportion as the circular arc reaction tank 13, and the bottom of each section of the circular arc cavity 23 is provided with the groove liquid guide tube 15; a reverse wedge ring slot 26 is also arranged below the groove liquid guide tube 15.

As a preferred embodiment of the present invention, the circular ring kit 12 is further configured as two box bodies distributed inside and outside, wherein the inner layer is configured as a small circular ring kit 62 with a reduced single structure, the periphery of the small circular ring kit 62 is provided with a circular ring reservoir 63 for increasing the addition amount of the reagent, the periphery of the circular ring reservoir 63 is further uniformly provided with primers and probes 64, and each of the primers and probes 64 is embedded in the circular arc cavity 23 at a corresponding position.

As a preferred embodiment of the present invention, the disk cover 5 is a circular disk with a shaft hole 8, and a hexagon nut 27 and a positioning pin 28 arranged on the edge of the shaft hole are arranged at the shaft hole of the disk cover 5;

a circular hole ring 29, a liquid inlet socket 30, a disc cover spigot 31 and a disc cover buckle 32 are sequentially arranged along the shaft hole 8 outwards, and the outer hexagonal inverse-tooth nut 27, the positioning pin 28, the disc cover spigot 31 and the disc cover buckle 32 are used for cooperating together and accurately embedding and fixing the disc cover 5 on the chassis 4 of the clamping disc 2; the circular hole ring 29 is used for being inserted into a plurality of nozzles to input a plurality of reagents respectively, positive pressure gas is input from the air pump 33, and the liquid inlet socket 30 is used for being inserted into the liquid inlet funnel 56 to input sample liquid.

As a preferred embodiment of the present invention, the liquid inlet funnel 56 is provided with an oval open upper portion, a funnel-shaped middle portion, and a conical through pipe at the outlet end of the lower portion, and is used for collecting oral sputum samples, the open opening of the liquid inlet funnel 56 is provided with an oval reagent pack plug 61 which has the same size and can be plugged into an extrusion type plastic reagent pack, the inner side edge of the open opening of the liquid inlet funnel 56 is provided with a needle-punched member 57, and a fiber filter layer 58 is padded in the middle of the liquid inlet funnel 56; two sides of the conical pipe at the lower part of the liquid inlet funnel 56 are provided with fascia strips 59 which correspond to the liquid inlet sockets 30 in size and are fixedly arranged at the upper part of the conical pipe, and the lower parts of the fascia strips 59 are movable strip blocks used for locking and stopping the left side and the right side of the liquid inlet funnel 56; the reagent pack plug 61 is provided with virus lysate, and the needling member 57 is used for inserting and puncturing the extrusion type plastic reagent pack, so that the virus lysate is filled to wash viscous sputum filtered and blocked by the fiber filter layer 58.

As a preferred embodiment of the present invention, the conical chip layer 3 is provided with a thermoplastic resin material with a size consistent with that of the conical chip layer as a chip base layer 34, a PET coil is adhered to the outside of the chip base layer 34 as a chip cover layer 35 of the conical chip layer 3, and a PE coil is adhered to the outside of the chip cover layer 35 as a chip protection layer 36 of the conical chip layer 3;

the conical chip layer 3 is also provided with a single-side chip layer or double-side chip layers; the single-side chip layer is provided with at least four groups of screening channels 7 only on the outer surface of the chip base layer 34; the double-side chip layer is characterized in that more than four groups of screening channels 7 are respectively arranged on the inner side surface and the outer side surface of the chip base layer 34.

As a preferred embodiment of the present invention, the chip cover layer 35 is manufactured with a plurality of large hole ends 52 and small hole ends 53 with preset sizes by slitting and edge-pressing curling, each of the large hole ends 52 and the small hole ends 53 is respectively arranged at the upper closed circular ring end and the lower closed circular ring end of the conical chip layer 3, and each of the large hole ends 52 and the small hole ends 53 is set to be in a closed state;

at least more than four liquid inlet pipes coated with hot melt quick drying glue layers are equidistantly arranged on the circumference of the upper closed circular ring end of the small hole end 53; each section of the conical chip layer 3 is also provided with a group of notches coated with hot-melt quick-drying adhesive along the height direction of the conical chip layer 3, and each section of the conical chip layer 3 is curled to form each section of the conical chip layer 3 after the notches are butted and bonded;

the small hole end 53 of the conical chip layer 3 is inserted into the reversed wedge ring slot 26, the groove liquid guide tube 15 is inserted into the corresponding liquid inlet pipe 37, the end surface of the small hole end 53 of the conical chip layer 3 is coated with a hot melt adhesive 54, and the hot melt adhesive 54 is used for bonding the reversed wedge ring slot 26 arranged on the bottom of the chassis 4, so as to realize the matching assembly of the bowl-shaped chip 1.

As a preferred embodiment of the present invention, a preset number of screening channels 7 are disposed on the conical chip layer 3, wherein the screening channels 7 are disposed vertically along the conical chip layer 3 or in a parabolic manner along a rotation direction of the conical chip layer 3, each screening channel 7 is respectively provided with a liquid inlet pipe 37, at least one or more than one shunt channel 38, a backflow preventing buckle 39, a pre-assembly port 40, an upper blocking channel 41, a lower blocking channel 42, a square chromogenic groove 45 and a waste liquid groove 46, inlet diameters of the upper blocking channel 41 and the lower blocking channel 42 are both set to be larger than a diameter of the square chromogenic groove 45, the upper blocking channel 41 and the lower blocking channel 42 are communicated with the square chromogenic groove 45, the waste liquid grooves 46 are disposed on two sides of the square chromogenic groove 45, and a piece of absorbent paper 47 is disposed between the square chromogenic groove 45 and the waste liquid groove 46, an exhaust groove 49 is arranged above the waste liquid tank 46, the waste liquid tank 46 is communicated with the exhaust groove 49 through an anti-overflow pipeline 48, an exhaust hole 51 is also arranged on the exhaust groove 49, and the exhaust hole 51 is used for exhausting gas existing in a pipeline of the bowl-shaped chip 1 in the processes of inputting, vibrating, centrifuging and positive pressure of a liquid sample;

and the waste liquid groove 46 is pre-embedded with a high molecular water absorbing material, the upper blocking channel 41 and the lower blocking channel 42 are provided with microspheres 43 which are filled in the pre-assembly port, the microspheres 43 are used for preventing the reagent from being blocked, the bottoms of the upper blocking channel 41 and the lower blocking channel 42 are provided with anti-falling buckles 44, and the anti-falling buckles 44 are used for preventing the microspheres 43 from falling.

This automation equipment based on centrifugal micro-fluidic chip of bowl form realizes virus screening, wherein, automation equipment contain the aforesaid bowl form chip 1, clamping dish 2, circular cone chip layer 3, chassis 4 and lid dish 5, wherein, bowl form chip 1 adopt the bull peristaltic pump follow the top of bowl form chip 1 annotate the liquid and handle, just automation equipment still be provided with feed liquor funnel 56, aerosol sample thief 65, medium temperature sterilization case 68 and screening window baffle 69.

The desktop device for realizing virus screening based on the bowl-shaped centrifugal microfluidic chip comprises the bowl-shaped chip 1, a clamping disc 2, a conical chip layer 3, a base plate 4 and a cover disc 5, wherein the bowl-shaped chip 1 adopts a mode of extracting from a bottom box by adopting an intelligent vacuum pump and a water pump and injecting liquid from the top of the bowl-shaped chip 1, the bowl-shaped chip 1 is formed by superposing a cylindrical outer sleeve 87, an inner sleeve 92 and a disc-shaped bottom box 86, a color sensor 88 is arranged on the inner wall of the outer sleeve 87, an arc-shaped screen 89 is attached to the arc of the outer wall of the outer sleeve 87, and arc-shaped nozzles 90 and an outer sleeve handle 91 in preset numbers are arranged in the top of the outer sleeve 87; the center of the top of the inner sleeve 92 is provided with a micro motor 93, a micro motor shaft 94 is arranged in the vertical upward protruding direction of the micro motor 93, a positioning turntable 95 is arranged above the micro motor shaft 94, the positioning turntable 95 comprises a rotating disc 95-1, a fixed disc 95-2 arranged at the bottom of the rotating disc 95-1 and a positioning steel ball 95-3 arranged on the fixed disc 95-2, and the rotating disc 95-1 is provided with the bowl-shaped chip 1.

As a preferred embodiment of the present invention, the bottom case 86 includes a bottom cover 96 and a base 103, the bottom cover 96 is provided with a large insertion buckle 97 and a small insertion buckle 98, the large insertion buckle 97 is used for inserting and fixing the outer sleeve 87, and the small insertion buckle 98 is used for inserting and fixing the inner sleeve 92; the central position of the bottom cover 96 is provided with an intelligent vacuum water pump 99, a micro water pump A100, a micro water pump B101 and a control electric plate 102; the bottom cover 96 is arranged on a base 103, the base 103 is also provided with a foot seat 104, and a storage area A105, a storage area B106 and a storage area C107 for storing liquid reagents are arranged in the base 103 in a dividing way; the intelligent vacuum water pump 99, the miniature water pump A100 and the miniature water pump B101 are connected with the storage area A105, the storage area B106 and the storage area C107 through a suction pipe and connected with the arc-shaped nozzles 90 through plastic pipes, and the control electric plate 102 is electrically connected with the intelligent vacuum water pump 99, the miniature water pump A100, the miniature water pump B101, the color sensor 88, the arc-shaped screen 89 and the miniature motor 93.

The wearable device for screening viruses based on the bowl-shaped centrifugal microfluidic chip comprises the bowl-shaped chip 1, a clamping disc 2, a conical chip layer 3, a base plate 4 and a cover disc 5, wherein the bowl-shaped chip 1 is divided into intervals with preset numbers in a circular kit 12, screening reagents are filled in advance and are arranged in a positive pressure mode of manually pressing through a reagent channel and blowing through a mouth, a function switch, a display screen and a corresponding display and electric plate 110 are selected by an operation switch button 109 of the wearable device 108, and screening is realized in a mode of pressing the wearable device 108 on a vertical cylindrical wearable base 111 in a sleeved mode to vertically run.

The bowl-shaped centrifugal microfluidic chip (hereinafter referred to as a bowl-shaped chip 1; see fig. 1 and 2) in the automatic device is a clamping disk 2 (see fig. 7) which is formed by embedding a base disk 4 (see fig. 3 and 4) and a cover disk 5 (see fig. 5 and 6) and is similar to a bowl bottom disk; the hollow conical chip layer (hereinafter, referred to as a conical chip layer 3; please refer to fig. 8 and 9) with a microporous wall structure is arranged like a hollow cone on the bowl edge, a liquid inlet channel 6 (please refer to fig. 9) is arranged on the clamping disc 2, and the liquid inlet channel 6 is formed by a circular ring kit 12, an arc reaction tank 13, a circular pipe groove 14, a groove liquid guide pipe 15, a circular pipe cavity 25, a reversed wedge ring slot 26 and a liquid inlet socket 30; the conical chip layer 3 is provided with a parallel independent micropore array screening channel 7 (please refer to fig. 10), the screening channel is formed by the liquid inlet pipe 37, the shunt channel 38, the backflow prevention buckle 39, the pre-assembly port 40, the upper blocking channel 41, the lower blocking channel 42, the microspheres 43, the anti-falling buckle 44, the square color developing groove 45, the waste liquid groove 46, the absorbent paper 47, the anti-overflow pipeline 48 and the exhaust groove 49, the conical chip layer 3 is embedded with the clamping disc 2 to form a three-dimensional intersected bowl-shaped chip 1, and the center of the clamping disc 2 is inserted with a motor shaft for realizing centrifugal mixing of the bowl-shaped chip 1.

The clamping disc base plate 4 is a flat-bottom disc with the diameter phi of 150mm and the height of 18mm (refer to fig. 3), and a concave opening 8 with the depth of 2mm and the diameter phi of 200mm is arranged at the upper end of the base plate 4; a central cylinder 9 with the height of 12mm and the diameter of phi 30mm, a straight-through shaft hole 10 with the diameter of phi 8mm for inserting a motor and two positioning pin holes 11 with the diameter of phi 3mm are sequentially arranged in the disc; and a ring kit 12 with a height of 9mm, an upper end diameter phi 90mm and a lower end diameter phi 80 mm; a circular arc reaction tank 13 with the height of 10mm and the diameter phi of 116mm, a round pipe groove 14 with the diameter phi of 9mm, and a groove liquid guide pipe 15 with the diameter phi of 3mm below the round pipe groove 14; a chassis clamping groove 16 of a bayonet is arranged at the circumferential edge of the upper end of the chassis 4; the bottom of the chassis 4 is provided with an inwards concave bottom 17; the positioning pin holes 11 and the chassis clamping grooves 16 are used for positioning and correspondingly embedding and clamping the clamping plate cover 5, the liquid inlet channel 6 (please refer to fig. 3, 4 and 5) of the bowl-shaped chip 1 is arranged on the plane layer of the clamping plate 2 outside the bowl bottom, and the liquid inlet channel 6 sequentially comprises a liquid inlet socket 30, a circular ring kit 12, an arc liquid storage tank 13, a circular pipe groove 14 and a groove liquid guide pipe 15.

The annular reagent box 12 is arranged to be closed at the inner side, the periphery of the outer side is provided with a reagent box inclined plane 19, the top of the box body protrudes outwards, and the bottom of the box body inclines inwards to prevent reagent from splashing; the bottom surface 20 of the reagent box is arranged to be high in the center and low in inclination towards the circumference so as to facilitate the flowing of the reagent; the annular reagent box 12 is equally divided into eight sections of annular cavities 21 according to angles, and the annular cavities 21 are divided into three input reagents and a fan-shaped grid 22 for inputting gas.

The arc reaction tank 13 is equally divided into eight arc cavities 23 according to the proportion, the length/width/depth of the eight arc cavities 23 is 35/17/10mm, and the eight arc cavities can contain 1-6 ml of reaction liquid, and a screening virus primer 24 is pre-embedded in each arc cavity 23 to detect pathogens of the eight arc cavities; then, dividing the circular tube groove 14 with the diameter phi of 9mm into eight circular tube cavities 25 and connecting the eight circular tube cavities with the periphery of each section of circular arc cavity 23, wherein the bottom of each section of circular tube cavity 25 is provided with a groove liquid guide tube 15 with the length of 3mm and the diameter phi of 2.5 mm; a reversed wedge ring slot 26 with the width of 10mm is also arranged below the groove liquid guide pipe 15.

The clamping disc cover 5 is a disc with a shaft hole 8, an outer hexagonal inverse-tooth nut 27 and a positioning pin 28 at the edge of the shaft hole are arranged at the shaft hole of the disc cover 5, a circular hole ring 29, a liquid inlet socket 30, a disc cover spigot 31 and a disc cover buckle 32 are sequentially arranged outwards, and the nut 27, the positioning pin 28, the disc cover spigot 31 and the disc cover buckle 32 which are arranged on the shaft hole 8 are used for accurately embedding and fixing the disc cover 5 on the chassis 4 of the clamping disc 3; the circular hole ring 29 is used for inserting a plurality of nozzles to input various reagents respectively and inputting positive pressure gas from the gas pump 33; the inlet spigot 30 is adapted to be inserted into an inlet funnel 57 for the input of sample fluid.

The conical chip layer 3 (refer to fig. 4 and 5) with the microporous wall structure is provided with thermoplastic resin, coiled materials with the same size, a chip base layer 34 serving as the conical chip layer 3, a layer of PET coiled material adhered outside the chip base layer and serving as a chip cover layer 35 of the chip layer, and a layer of PE coiled material adhered outside the chip cover layer 35 and serving as a chip protective layer 36 of the chip layer (the single-sided chip in the scheme only adopts a unidirectional outer layer), the surface of the chip base layer 34 is respectively provided with eight groups of screening channels 6 with microstructures, and the structure of each screening channel 6 is sequentially set as follows:

(1) a liquid inlet pipe 37 (phi 3mm, length 5 mm);

(2) a flow dividing channel 38 (two strips are 5/3/2mm in length, width and thickness and form an angle of 45 degrees);

(3) an anti-backflow buckle 39 (the two widths and the thicknesses are respectively 0.4/0.4 mm);

(4) a pre-assembly opening 40 (the length, width and thickness of each of the two diamonds are 5/5/1 mm);

(5) an upper blocking passage 41 (0.5/0.5 mm wide and 1/4 mm long, respectively);

(6) a lower plugging channel 42 (width and thickness of 0.4/0.4mm, length of 3/4 mm, and the lower half part of the channel is a blocking section);

(7) microspheres 43 (30 μm in diameter, pre-filled from pre-assembly port 40 for pre-plugging);

(8) anti-falling buckles 44 (provided to prevent microspheres from falling);

(9) a square color developing tank 45 (two 10/10/2mm in length, width and thickness, respectively, and capable of containing 0.2ml of solution);

(10) a waste liquid tank 46 (length, width and thickness: 15/10/2 mm);

(11) absorbent paper 47 (length, width and thickness 8/5/0.3);

(12) the overflow prevention pipe 48 (12/2/2 in length, width and thickness);

(13) an exhaust groove 49 (diameter of 4 mm/thickness of 2 mm/height of 10 mm);

after the chip base layer 34 is covered with a PET chip cover layer 35 after processing and manufacturing, a two-dimensional code 50 is printed above the exhaust groove 49 of the chip base layer 34, and an exhaust hole 51 communicated with the outside is punched at the exhaust groove 49, wherein the exhaust hole 51 is used for discharging gas in a pipeline of the bowl-shaped chip 1 in the processes of inputting, vibrating, centrifuging and positive pressure of a liquid sample.

Subsequently, a chip protection layer 36 made of PE transparent materials is overlaid and adhered on the surface of the PET chip cover layer 35; finally, the single width is 60mm, the small hole end 52 is about phi 130mm, and the large hole end 53 is about phi 160mm through slitting and edge pressing and curling; and the edge of the small hole end 52 is also coated with a hot melt adhesive layer 54 with the length of 10 mm. In addition, a chip interface 55 along the height direction of the conical chip 3 is further arranged on the surface of the conical chip layer 3, and the conical chip layer 3 is formed after the coiled material is glued, adhered and curled.

Then the small hole end 52 of the conical chip layer 3 is inserted into the reversed wedge ring slot 26 at the bottom of the clamping disk chassis 4, the groove liquid guide tube 15 is correspondingly inserted into the liquid inlet pipe 37, and the hot melt adhesive 54 coated on the end face of the small hole end 52 of the conical chip layer 3 is completely bonded with the reversed wedge ring slot 26 at the bottom of the clamping disk chassis 4, so that the matching assembly of the bowl-shaped chip 1 is realized.

The liquid inlet funnel 56 is provided with an oval opening shape with the upper part height of 15mm and the phi of about 30mm, the middle part is funnel-shaped, the outlet end of the lower part is a conical through pipe, the opening of the liquid inlet funnel 56 is provided with an oval plug 61 which has the same size and can be plugged into an extrusion type plastic reagent bag, the edge of the inner side of the opening of the liquid inlet funnel 56 is provided with a needling component 57, and the middle pad is provided with a fiber filter layer 58; two sides of a conical pipe at the lower part of the liquid inlet funnel 56 are provided with reinforced membrane strips 59 which have the size corresponding to the liquid inlet sockets and are fixed at the upper parts, and the lower parts of the reinforced membrane strips 59 are provided with movable strip blocks 60 for locking and stopping two sides of the open funnel; the reagent pack plug 61 is internally provided with virus lysate, the liquid inlet funnel 56 is used for collecting a sample of oral sputum, and the needling component 57 is used for inserting and puncturing the plastic reagent pack 61 so as to perfuse the virus lysate to wash the filtered viscous sputum on the fiber filter layer 58.

When screening is carried out, three reagent nozzles and one gas nozzle are respectively inserted into four round holes in the round hole ring 23 on the cover disc in the direction of the circle center of the kit and are respectively injected into the four fan-shaped grids 22. Firstly, 30 muL of alcohol and 15 muL of ammonium acetate are respectively sprayed into the two fan-shaped grids, after the alcohol and the ammonium acetate are centrifugally mixed, 280 muL of red oily dye is sprayed, after the red oily dye and the ammonium acetate are centrifugally mixed again, positive pressure gas introduced from the air pump 24 is input through the spray head inserted into the fourth fan-shaped grid 22.

At present, epidemic situation research is mainly carried out by single pneumonia detection caused by new coronavirus, but various viruses causing respiratory virus infection, such as respiratory syncytial virus, rhinovirus, adenovirus and metapneumovirus, as well as influenza virus, parainfluenza virus, coronavirus and coxsackie virus. In order to solve the problem that the infection symptoms of the virus influenza and the common cold are similar, and avoid the large-scale infection risk caused by the fact that repeated detection is labor-consuming and time-consuming, the cost is too high and virus omission detection is avoided, particularly in cold seasons, a plurality of respiratory virus detection reagents are combined to screen suspected cases, the disease classification of patients is carried out rapidly, and the method has very important significance for achieving rapid isolation and targeted treatment. The scheme is provided with another technical scheme.

As another preferred embodiment of the present invention, please refer to FIGS. 13 and 14, which are based on the "blocking flow by physical precipitation of nucleic acid" technology and employ a bowl-shaped centrifugal microfluidic (field-vision) chip (similar to the former case will not be described again). The difference lies in that the circular kit 12 is divided into an inner box body and an outer box body by the scheme:

1. a small circular kit 62 with a reduced single structure (three reagents, one gas channel) on the inner layer is provided;

2. the amount of the reagent added to the small circle kit 62 is increased (doubled as the reagent in the first embodiment);

3. a circular liquid storage tank 63 (arranged on the periphery of the small circular reagent box 62) is added;

4. eight primers and probes 64 aiming at different respiratory viruses are added and respectively embedded in the arc cavities 23 which are divided into eight different parts;

5. the method realizes the simultaneous screening of multiple target viruses on a single sample by adopting the same screening mode of the first embodiment.

The embodiment of the technical scheme is based on the detection result that the oily colored indicating liquid can be visually detected to be positive after the detection layer blocks a channel at the section under the positive pressure action when the precipitation diameter is larger than the size of the gap formed by stacking the microspheres.

After the mixed solution of ethanol and ammonium acetate is introduced into the bowl-shaped chip 1, the bowl-shaped chip 1 starts the centrifugal motor again to realize centrifugation for 30s, and the alcohol is rapidly mixed with the reaction system in the centrifugation process, so that the nucleic acid precipitate is uniformly generated. In order to make the reaction result better observed, 280 μ L of red oil dye is injected into the clamping plate 2, so that the result of detection can be rapidly observed by naked eyes, and positive pressure driving is given to the bowl-shaped chip 1 at room temperature.

The bowl-shaped centrifugal microfluidic chip is applied as follows, wherein technical features which are not described in detail are the same as those in the above description, and are not described again here.

The bowl-shaped chip 1 arranged in the automatic device of the first automatic device comprises the following operation processes:

1. an aerosol sampler 65 is added (a screener enters a room, and the aerosol sampler 65 and the negative pressure exhaust fan 66 are started immediately);

2. sampling is completed by adding a liquid inlet funnel 56 (a disposable mask with a hole in the center is inserted), and plugging a plug (virus lysate is squeezed in) after blowing and spitting;

3. after the screener finishes sampling once and presses the fingerprint controller:

firstly, the bowl-shaped chip 1 rotates at an angle immediately, and the screening window baffle 69 can expose the unused liquid inlet socket 30;

secondly, the liquid inlet funnel 56 wrapping the disposable mask after sampling is subjected to a heating, baking and sterilizing procedure through a medium temperature sterilizing box 68;

thirdly, if each sampling socket of the bowl-shaped chip 1 is used up, the sampling socket is automatically lifted and leveled up to enter a horizontal conveying state;

4. when the bowl-shaped chip 1 reaches the horizontal conveying position, the multi-head peristaltic pump 81 is started immediately, the circular nozzle 82 (comprising 8 groups of 32 nozzles, wherein each group comprises 3 reagent nozzles and 1 gas nozzle) is descended and inserted into the circular nozzle 82 at the outer side of the reagent kit to input a reagent (the reagent comprises 30 mu l of alcohol, namely ammonium acetate 2: 1 mixed sputum solution to the circular arc reaction tank 13), the plug circular nozzle 82 is lifted immediately, and the reagent is centrifuged quickly for 30 s; then, 20 mu L of oily colored indicating solution flows into each arc reaction tank, and then the mixture is centrifuged for 30 s;

5. starting the micro air pump 80 to inflate the arc reaction tank of the bowl-shaped chip 1 to generate positive pressure to drive the sample to flow;

6. whether or not the colors of the "reaction tank clogging section channel and the coloring section channel" are abnormal is checked by the color sensor 88 (or visually checked).

After the bowl-shaped chip 1 is collected, the system automatically lifts and lays the bowl-shaped chip 1, and the multi-head peristaltic pump immediately aligns with the circular hole ring 29 on the bowl-shaped chip 1 to input a corresponding reagent and quickly carry out centrifugal rotation; rotate mix reagent 30s after and promptly stop fast, bull peristaltic pump 81 input take the colored reagent again after rotate 30s stop fast again, miniature air pump 80 blow in malleation gas fast to bowl form chip 1 in, and slowly rotate bowl form chip 1, by video camera to bowl form chip layer observation area of side in chip 1 screen piece by piece whether the color is unusual? Once the abnormity is found, the system uploads the two-dimensional code at the abnormal position to a cloud server through a signal emitter, the cloud server analyzes, checks and authenticates, a check report of the new coronavirus nucleic acid reagent of the testee is sent to a system center and a mobile phone end of the tested person, and meanwhile, the system also gives an alarm through voice and light; if the negative pressure suction fan 66 of the system detects virus information by an air (including epithelial cells and metabolites) sensor in the screening room, an alarm message is also sent by a voice player and a light.

A bowl-shaped wearable centrifugal (visually discriminable) microfluidic chip, the use comprising the steps of:

step 1, after the ID card and the video are authenticated, a testee obtains a specific two-dimensional code to open a door and enter screening;

step 2, taking a disposable (with a hole in the center) mask, and inserting a liquid inlet funnel 56 into the center hole of the disposable mask;

step 3, pulling out a liquid inlet plug at a liquid inlet of the clamping disc, inserting the liquid inlet plug into a liquid inlet funnel with a mask and wearing the liquid inlet funnel on the face;

step 4, biting a liquid inlet funnel, blowing air, then promoting spitting of sputum, and then plugging a plug to squeeze in the virus lysate;

step 5, the mask is taken off, the mask is tightly tied on the liquid inlet funnel 56 by using an ear rope, and the mask is taken out after the fingerprint controller is pressed;

step 6, rotating the bowl-shaped chip 1 by an angle, displaying an unused liquid inlet plug, and continuously sampling by a later person;

step 7, after sampling is finished, immediately lifting and flatly placing the bowl-shaped chip 1, and entering a screening process of horizontal conveying;

step 8, inputting a reagent into the circular hole ring 29 on the bowl-shaped chip 1 by the multi-head peristaltic pump, and quickly centrifuging for 30 s;

step 9, releasing 30 mul of alcohol by a multi-head peristaltic pump: ammonium acetate 2: 1, mixing the sputum solution to an arc reaction tank 13;

step 10, then flowing 20 mu L of oily colored indicating solution into each arc reaction tank, and centrifuging for 30 s;

step 11, starting an intelligent vacuum pump to inflate the arc reaction tank to generate positive pressure to drive a sample to flow;

and 12, checking whether the colors of the reaction tank blocking section channel and the color development section channel are abnormal or not by visual inspection.

The bowl-shaped chip 1 of the second desktop type device 85 adopts the mode that the size is consistent with that of an automatic bowl-shaped chip, an intelligent vacuum pump and a water pump are adopted for extracting from a bottom box and injecting liquid from the top of the bowl-shaped chip, and the bowl-shaped chip is formed by superposing a cylindrical outer sleeve 87, an inner sleeve 92 and a disc-shaped bottom box 86, wherein the inner wall of the outer sleeve 87 is provided with a color sensor 88, an arc-shaped screen 89 is attached to the arc of the outer wall, and arc-shaped nozzles 90 (four side by side) and an outer sleeve handle 91 are arranged in the top; a power supply 93 and a miniature motor shaft 94 which protrudes vertically upwards are arranged at the center of the top of the inner sleeve 92, a positioning turntable 95 (a rotating disc 95-1// a fixed disc 95-2 at the bottom of the rotating disc// a positioning steel ball 95-3 arranged on the fixed disc) and a bowl-shaped chip 1 arranged on the rotating disc 95-1 are arranged above the miniature motor shaft 94;

the bottom box 86 comprises a bottom cover 96 and a base 103, wherein the bottom cover 96 is provided with an inserting buckle 97 and an inserting buckle 98, the inserting buckle 97 is used for inserting and fixing the outer sleeve 87, and the inserting buckle 98 is used for inserting and fixing the inner sleeve 92; the center of the bottom cover 96 is provided with an intelligent vacuum water pump 99, a micro water pump A100, a micro water pump B101 and a control electric plate 102; the base 103 is provided with a foot seat 104, and the base is internally divided into a storage area A105, a storage area B106 and a storage area C107 of three liquid reagents; the intelligent vacuum water pump 99, the micro water pump A100 and the micro water pump B101 are connected with the 3 liquid reagent storage areas A105, B106 and C107 by suction pipes and are connected with the four nozzles 90 which are arranged in an arc shape by plastic pipes; the control electric plate 102 is electrically connected with the intelligent vacuum water pump 99, the micro water pump A100, the micro water pump B101, the micro color sensor 88, the arc-shaped screen 89 and the micro motor 93.

The bowl-shaped chip 1 arranged in the third wearable device 108 of the present invention adopts a chip with a diameter phi of 50 x 80 and a height of 22 mm (please see fig. 25, 26, 27, 28, 29), and adopts a positive pressure mode that three screening reagents are pre-filled into the reagent kit 12 in four intervals, pass through a manual pressure reagent channel and blow through the mouth, and the wearable device 108, the operation keys 109 select a function switch, a display screen and a control circuit and a power supply 110 behind the display screen, and the wearable device is sleeved on a vertical cylinder to vertically run by adopting the vertical cylinder 111.

At present, a virus screening mode by collecting nasopharyngeal swabs in a large scale has great influence on screening results in the aspects of skills, infection time and the like, and the heating amplification of the virus is very time-consuming during detection. The sputum is used as a sample for screening the viruses by utilizing the advantage that the content of the viruses in the sputum is far beyond the mucous membrane at the nasopharynx, so that the sample to be detected does not need to be heated and amplified; secondly, professional operators are not needed, and errors caused by human can be reduced; third, there is no aerosol pollution; the method can obviously accelerate the screening speed, increase the specificity and sensitivity of detection, can fully mix the solution only by 3 minutes of mechanical operation, accurately divide the sample after centrifugation, and rapidly push the separation of the sample by positive pressure driving so as to realize full-automatic operation and visual rapid detection of multiple targets of the sputum sample.

The liquid inlet funnel 56 is used for blowing air with a nozzle, mustard powder is added on the surface of the honeycomb sponge 58 to cause stimulation to cause cough, so that an air passage can vibrate during blowing to generate severe cough to loosen secretion in the air passage, thick phlegm adhered to the air passage is loosened and easy to discharge (has the same phlegm discharging effect as a vibrating phlegm discharging device), loosened secretion (epithelial cells and metabolites) in an air pipe and an air alveolus is pushed into an upper air passage from bottom to top, and the lung environment is improved to facilitate the cough of the secretion.

The scheme adopts and imbeds honeycomb sponge in feed liquor funnel 56, relies on the super large area of contact of honeycomb sponge to remove the high-efficient separation and adsorb the viscidity sputum in the sample, simultaneously with the help of the impulsive force of "virus lysate" in the extrusion feed liquor funnel 56 opening is plugged into lid 61, makes thin mucus sample in the sputum flow to the reservoir smoothly through the space to guarantee virus lysate to the dissolution and the release of virus nucleic acid, therefore realize the successful collection of sputum sample.

The present case adopts the setting of paper that absorbs water in the waste liquid groove, adsorbs unnecessary detection liquid through the paper that absorbs water to avoid detecting liquid and spill over in a large number, improve the security of sample.

The scheme adopts the virus screening method that the face image and the 'ID card' are identified simultaneously and the two-dimensional code of the tested person on the bowl-shaped chip 1 is identified, and after the Bluetooth device on the screening device is associated with the APP software on the mobile phone, the data can be uploaded to the epidemic situation monitoring system stored on the network big data platform, and meanwhile, the data is uploaded to the mobile phone of the user. Epidemic situation and virus data service system through collect, arrangement, filtration, judgement, analysis, feedback carry out the high in the clouds and share and show swiftly, judge for the government to the epidemic situation and do detailed reference. The introduction of large data will make the screening device truly an extension of human monitoring functions, and its importance will certainly become increasingly prominent. Boxing is to be performed in virus screening or epidemic situation monitoring.

In the embodiment of the present invention, a screening scheme using "ethanol solution and nucleic acid" reagent is mainly derived from the technology disclosed in CN 113652341A: the reagent comprises a combined reagent of ethanol solution, ammonium acetate and oily red indicator liquid, and a simple flow channel and visual detection. However, in order to improve the efficiency and effect of the scheme, a better technical scheme of 'direct sample collection, reagent pre-embedding, pneumatic positive pressure' and 'bowl-shaped chip 1 set centrifuge' is provided, so that the large-amplitude improvement and the full presentation of the using effect in the prior art are realized.

Meanwhile, the chip adopts an integrated and disposable design, the reagent dosage is less, the integration level is high, related primers and probes are pre-embedded in advance and are adhered to the bowl-shaped conical chip, cross mixing and dissolving are avoided in the chip, and the environment is not polluted. Centrifugal force in this chip can guarantee that the sputum sample can intensive mixing and flow into each function groove smoothly, can accomplish the collection and the detection of sputum sample fast in the chip of relative closed to can directly visually confirm the testing result, promote the portability that detects greatly, more need not to examine the sample censorship and carry the effect when contracting. The bowl-shaped centrifugal microfluidic chip has the characteristics of convenience, easiness in manufacturing, high sensitivity, stability, low cost, high sensitivity, high efficiency, high flux, high automation degree and the like, improves the control capability of the existing microfluidic chip on fluid, realizes the detection and analysis of multifunctional microfluidic samples, has a very large market in the aspect of expanding the application of the microfluidic technology entering families, and has extremely good application value in the field of POCT detection.

Compared with the prior art, the beneficial effects brought by the invention are shown in the table:

in the description herein, references to the description of terms "an embodiment," "some embodiments," "an example," "a specific example," or "an embodiment," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

The bowl-shaped centrifugal microfluidic chip for screening viruses by adopting the sputum is used as a noninvasive, efficient and convenient airport customs and home direct sputum collection and detection mode, and multiple reactions of a conventional analysis and detection method are integrated on the bowl-shaped centrifugal microfluidic chip. The kit can be widely applied to detection of various respiratory viruses, can obviously improve the accuracy and the detection speed of detection results, and can screen patients with no symptoms or at the early stage of disease, thereby avoiding the risk of cross infection. Meanwhile, the device is suitable for operation and carrying, simple, convenient, efficient, economical and durable in production and manufacture, and is very suitable for large-scale popularization and application.

In this specification, the invention has been described with reference to specific embodiments thereof. It will, however, be evident that various modifications and changes may be made thereto without departing from the broader spirit and scope of the invention. The specification and drawings are, accordingly, to be regarded in an illustrative rather than a restrictive sense.

Claims

1. A device for realizing virus screening based on a bowl-shaped centrifugal microfluidic chip is characterized by comprising:

a clamping disc (2) which is embedded with the cover disc (5) through the chassis (4);

the conical chip layer (3) is matched with the clamping disc (2) and is provided with a hollow cone with a microporous wall structure;

the conical chip layer (3) is embedded with the clamping disc (2) to form a bowl-shaped chip (1) which is arranged in a three-dimensional intersecting manner; and a motor shaft is inserted in the center of the clamping plate (2) and used for realizing centrifugal mixing treatment on the bowl-shaped chip (1).

2. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip is characterized in that the base plate (4) is a flat-bottomed disc, a concave opening (8) is formed in the upper end of the base plate (4), a central cylinder (9), a through shaft hole (10) for inserting a motor and a positioning pin hole (11) formed in the edge of the through shaft hole (10) are sequentially arranged in the base plate (4);

a chassis clamping groove (16) with a bayonet is formed in the circumferential edge of the upper end of the chassis (4), an inward concave bottom is formed in the bottom of the chassis (4), and the positioning pin hole (11) and the chassis clamping groove (16) are used for positioning and embedding the disc cover (5) of the clamping disc (2); and

the liquid inlet channel (6) of the clamping disc (2) is formed by combining a liquid inlet socket (30), a circular ring kit (12), a circular arc reaction tank (13), a circular tube groove (14) and a groove liquid guide pipe (15).

3. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip according to claim 2,

the box body of the circular kit (12) is made of plastic, and the annular outer vertical surface of the box body is provided with a structure with the upper end protruding outwards and the lower end inclining inwards, and is stuck and sealed by a film for preventing the reagent from splashing;

the bottom of the circular kit (12) is arranged to be of a structure which is high in center and inclined towards the circumferential direction, the circular kit (12) is arranged to be provided with N independent arc sections according to an angle, each arc section is divided into M fan-shaped grids (27) of independent fan grids, more than one powder or reagent and a channel for inputting gas are pre-buried in each fan-shaped grid (27), one end of the gas channel is communicated with the micro air pump, and the other end of the gas channel is communicated with the circular tube groove (14) and the groove through hole of the groove liquid guide tube (15).

4. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip according to claim 2,

a plurality of arc cavities (23) are uniformly arranged in the arc reaction tank (13) according to a proportion to contain reagents and reaction liquid, and at least more than one virus screening primer (24) is pre-buried in each arc cavity (23) and is used for detecting pathogens;

the circular tube grooves (14) are uniformly divided and arranged at the periphery of each section of the circular arc cavity (23) according to the same proportion as the circular arc reaction tank (13), and the bottom of each section of the circular arc cavity (23) is provided with the groove liquid guide pipe (15); a reverse wedge ring slot (26) is arranged below the groove liquid guide pipe (15).

5. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip as claimed in claim 4, wherein the ring kit (12) is further provided with two boxes distributed inside and outside, wherein the inner layer is provided with a small ring kit (62) with a reduced single structure, a ring reservoir (63) is arranged on the periphery of the small ring kit (62) for increasing the addition amount of reagents, primers and probes (64) are uniformly arranged on the periphery of the ring reservoir (63), and each primer and probe (64) is embedded in the corresponding circular arc cavity (23).

6. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip is characterized in that the tray cover (5) is a circular disc with a shaft hole (8), and a hexagon nut (27) and a positioning pin (28) arranged at the edge of the shaft hole are arranged at the shaft hole of the tray cover (5);

a circular hole ring (29), a liquid inlet socket (30), a disc cover spigot (31) and a disc cover buckle (32) are sequentially arranged outwards along the shaft hole (8), and the outer hexagonal inverse-tooth nut (27), the positioning pin (28), the disc cover spigot (31) and the disc cover buckle (32) jointly act to accurately embed and fix the disc cover (5) on the chassis (4) of the clamping disc (2); the circular hole ring (29) is used for being inserted into a plurality of spray heads to input a plurality of reagents respectively, positive pressure gas is input from the air pump (33), and the liquid inlet socket (30) is used for being inserted into the liquid inlet funnel (56) to input sample liquid.

7. The device for realizing virus screening based on the bowl-shaped centrifugal microfluidic chip according to claim 6, wherein the liquid inlet funnel (56) is provided with an oval open upper part, a funnel-shaped middle part and a conical through pipe at the outlet end of the lower part for collecting oral sputum samples, a reagent bag plug (61) which has the same size and is oval and can be plugged into an extrusion type plastic reagent bag is arranged at the open opening of the liquid inlet funnel (56), a needle punching component (57) is arranged at the inner side edge of the open opening of the liquid inlet funnel (56), and a fiber filtering layer (58) is padded at the middle position of the liquid inlet funnel (56); two sides of a conical pipe at the lower part of the liquid inlet funnel (56) are provided with ribbed membrane strips (59) which correspond to the liquid inlet sockets (30) in size and are fixedly arranged at the upper part of the conical pipe, and the lower parts of the ribbed membrane strips (59) are movable strip blocks used for locking and stopping the left side and the right side of the liquid inlet funnel (56); the reagent pack plug (61) is internally provided with virus lysate, and the needle puncturing component (57) is used for inserting and puncturing the extrusion type plastic reagent pack, so that the virus lysate is filled to wash viscous sputum filtered and blocked by the fiber filtering layer (58).

8. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip according to claim 1,

the conical chip layer (3) is provided with a thermoplastic resin material with the same size as the conical chip layer as a chip base layer (34), a layer of PET coiled material is pasted outside the chip base layer (34) as a chip cover layer (35) of the conical chip layer (3), and a layer of PE coiled material is pasted outside the chip cover layer (35) as a chip protection layer (36) of the conical chip layer (3);

the conical chip layer (3) is also provided with a single-side chip layer or double-side chip layers; the single-side chip layer is provided with at least more than four groups of screening channels (7) only on the outer surface of the chip base layer (34); the double-side chip layer is characterized in that more than four groups of screening channels (7) are respectively arranged on the inner side surface and the outer side surface of the chip base layer (34).

9. The device for screening viruses based on the bowl-shaped centrifugal microfluidic chip according to claim 8,

the chip cover layer (35) is provided with a plurality of large hole ends (52) and small hole ends (53) with preset sizes through slitting and edge pressing curling, the large hole ends (52) and the small hole ends (53) are respectively arranged at the upper closed circular ring end and the lower closed circular ring end of the conical chip layer (3), and the large hole ends (52) and the small hole ends (53) are set to be in a closed state;

at least more than four liquid inlet pipes coated with hot melt quick drying glue layers are equidistantly arranged on the circumference of the upper closed circular ring end of the small hole end (53); each section of the conical chip layer (3) is also provided with a group of notches coated with hot melt quick-drying adhesive along the height direction of the conical chip layer (3), and each section of the conical chip layer (3) is curled to form each section of the conical chip layer (3) after the notches are butted and bonded;

the small hole end (53) of the conical chip layer (3) is inserted into the inverted wedge ring slot (26), the groove liquid guide pipe (15) is inserted into the corresponding liquid inlet pipe (37), the end face of the small hole end (53) of the conical chip layer (3) is coated with hot melt adhesive (54), and the hot melt adhesive (54) is used for bonding the inverted wedge ring slot (26) arranged at the bottom of the chassis (4) so as to realize matching and assembling of the bowl-shaped chip (1).

10. The device for screening viruses based on bowl-shaped centrifugal microfluidic chips according to claim 9, wherein a predetermined number of screening channels (7) are disposed on the conical chip layer (3) and are disposed vertically along the conical chip layer (3) or in a parabolic manner along the rotation direction of the conical chip layer (3), each screening channel (7) is respectively disposed with a liquid inlet pipe (37), at least one or more fluid distribution channels (38), a backflow prevention buckle (39), a pre-assembly port (40), an upper blocking channel (41), a lower blocking channel (42), a square chromogenic groove (45) and a waste liquid groove (46), the inlet diameters of the upper blocking channel (41) and the lower blocking channel (42) are both set to be larger than the diameter of the square chromogenic groove (45), and the upper blocking channel (41) and the lower blocking channel (42) are communicated with the square chromogenic groove (45), the two sides of the square color developing groove (45) are provided with the waste liquid groove (46), water absorption paper (47) is arranged between the square color developing groove (45) and the waste liquid groove (46), an exhaust groove (49) is arranged above the waste liquid groove (46), the waste liquid groove (46) is communicated with the exhaust groove (49) through an anti-overflow pipeline (48), the exhaust groove (49) is also provided with an exhaust hole (51), and the exhaust hole (51) is used for exhausting gas in a pipeline of the bowl-shaped chip (1) in the processes of inputting, vibrating, centrifuging and positive pressure of a liquid sample;

and the waste liquid groove (46) is pre-embedded with a high-molecular water absorbing material, the upper blocking channel (41) and the lower blocking channel (42) are provided with microspheres (43) which are filled in the pre-assembly port, the microspheres (43) are used for preventing the reagent from blocking, the bottoms of the upper blocking channel (41) and the lower blocking channel (42) are provided with anti-falling buckles (44), and the anti-falling buckles (44) are used for preventing the microspheres (43) from falling.

11. An automatic device for realizing virus screening based on a bowl-shaped centrifugal microfluidic chip is characterized by comprising the bowl-shaped chip (1), a clamping disc (2), a conical chip layer (3), a base plate (4) and a cover disc (5) in claim 1, wherein the bowl-shaped chip (1) adopts a multi-head peristaltic pump to perform liquid injection treatment on the top of the bowl-shaped chip (1), and the automatic device is further provided with a liquid inlet funnel (56), an aerosol sampler (65), a medium-temperature sterilization box (68) and a screening window baffle plate (69).

12. A desktop device for realizing virus screening based on a bowl-shaped centrifugal microfluidic chip is characterized in that, the device comprises the bowl-shaped chip (1) of claim 1, a clamping plate (2), a conical chip layer (3), a bottom plate (4) and a cover plate (5), wherein, the bowl-shaped chip (1) is extracted from the bottom box by adopting an intelligent vacuum pump and a water pump, and the liquid is injected from the top of the bowl-shaped chip (1), the bowl-shaped chip (1) is formed by superposing a cylindrical outer sleeve (87), an inner sleeve (92) and a disc-shaped bottom box (86), a color sensor (88) is arranged on the inner wall of the outer sleeve (87), and the arc-shaped screen (89) is stuck on the arc of the outer wall of the outer sleeve (87), the top of the outer sleeve (87) is internally provided with a preset number of arc-shaped nozzles (90) and outer sleeve handles (91); inner sleeve (92) top center be provided with micro motor (93), and be in micro motor (93) perpendicular upwards the outstanding direction on be provided with micro motor shaft (94), the top of micro motor shaft (94) be provided with location carousel (95), location carousel (95) include rolling disc (95-1), set up fixed disk (95-2) in the bottom of rolling disc (95-1) to and install location steel ball (95-3) on fixed disk (95-2), rolling disc (95-1) on the installation be provided with bowl form chip (1).

13. The desktop device for screening viruses based on the bowl-shaped centrifugal microfluidic chip of claim 12, wherein the bottom case (86) comprises a bottom cover (96) and a base (103), the bottom cover (96) is provided with a large plug-in buckle (97) and a small plug-in buckle (98), the large plug-in buckle (97) is used for plugging and fixing the outer sleeve (87), and the small plug-in buckle (98) is used for plugging and fixing the inner sleeve (92); the central position of the bottom cover (96) is provided with an intelligent vacuum water pump (99), a micro water pump A (100), a micro water pump B (101) and a control electric plate (102); the bottom cover (96) is arranged on the base (103), the base (103) is also provided with a foot seat (104), and a storage area A (105), a storage area B (106) and a storage area C (107) for storing liquid reagents are arranged in the base (103) in a dividing way; intelligence vacuum water pump (99), miniature water pump first (100), miniature water pump second (101) pass through the straw with storage area first (105), storage area second (106) and storage area third (107) be connected to adopt the plastic pipe with each circular-arc nozzle (90) be connected, control electroplax (102) with intelligent vacuum water pump (99), miniature water pump first (100), miniature water pump second (101), color sensor (88), circular-arc screen (89) and micro motor (93) mutually the electricity be connected.

14. A wearable device for realizing virus screening based on a bowl-shaped centrifugal microfluidic chip is characterized by comprising the bowl-shaped chip (1) of claim 1, a clamping disc (2), a conical chip layer (3), a bottom disc (4) and a cover disc (5), wherein the bowl-shaped chip (1) is divided into intervals with preset numbers in a circular kit (12) and is pre-filled with screening reagents, the intervals are set in a positive pressure mode of manually pressing through a reagent channel and blowing through a mouth, a function switch, a display screen and a corresponding display and electric board (110) are selected by operating a switch key (109) of a wearable device (108), and the wearable device (108) is pressed on a vertical cylindrical base (111) to realize screening in a vertical operation mode.