CN115052995B - 分析方法、分析系统及分析表面 - Google Patents

分析方法、分析系统及分析表面 Download PDF

Info

Publication number
CN115052995B
CN115052995B CN202180012867.7A CN202180012867A CN115052995B CN 115052995 B CN115052995 B CN 115052995B CN 202180012867 A CN202180012867 A CN 202180012867A CN 115052995 B CN115052995 B CN 115052995B
Authority
CN
China
Prior art keywords
cell
molecule
analysis
specimen
stimulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202180012867.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN115052995A (zh
Inventor
松本真宽
中川和博
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sony Group Corp
Original Assignee
Sony Group Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sony Group Corp filed Critical Sony Group Corp
Publication of CN115052995A publication Critical patent/CN115052995A/zh
Application granted granted Critical
Publication of CN115052995B publication Critical patent/CN115052995B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN202180012867.7A 2020-02-14 2021-02-04 分析方法、分析系统及分析表面 Active CN115052995B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020023365 2020-02-14
JP2020-023365 2020-02-14
PCT/JP2021/004130 WO2021161893A1 (ja) 2020-02-14 2021-02-04 分析方法、分析システム、及び分析用表面

Publications (2)

Publication Number Publication Date
CN115052995A CN115052995A (zh) 2022-09-13
CN115052995B true CN115052995B (zh) 2025-05-06

Family

ID=77291444

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180012867.7A Active CN115052995B (zh) 2020-02-14 2021-02-04 分析方法、分析系统及分析表面

Country Status (5)

Country Link
US (1) US20230066282A1 (https=)
EP (1) EP4105340A4 (https=)
JP (2) JP7647736B2 (https=)
CN (1) CN115052995B (https=)
WO (1) WO2021161893A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117015710A (zh) * 2021-03-10 2023-11-07 索尼集团公司 生物粒子分析方法及用于生物粒子分析的试剂盒
CN116376662A (zh) * 2021-12-24 2023-07-04 映泰科技有限公司 空间转录组学分析的生物芯片和其制备方法及应用
US20250271415A1 (en) * 2022-03-29 2025-08-28 Sony Group Corporation Bioparticle analysis system, information processing device, and bioparticle analysis method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017019456A2 (en) * 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
WO2019079399A1 (en) * 2017-10-20 2019-04-25 Duke University DEVICES, SYSTEMS AND METHODS FOR HIGH SPEED SINGLE CELL ANALYSIS
EP3541956A1 (en) * 2016-11-17 2019-09-25 Spatial Transcriptomics AB Method for spatial tagging and analysing nucleic acids in a biological specimen

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7214477B1 (en) * 1999-07-26 2007-05-08 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Layered device with capture regions for cellular analysis
WO2001011341A2 (en) * 1999-08-05 2001-02-15 Cellomics, Inc. A system for cell-based screening
CA2814047C (en) * 2010-10-08 2017-11-14 President And Fellows Of Harvard College High-throughput immune sequencing
CN110079585A (zh) * 2012-07-30 2019-08-02 株式会社日立制作所 基因表达解析方法、基因表达解析用设备及基因表达解析装置
EP4592400A3 (en) * 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
ES2754177T3 (es) 2013-06-12 2020-04-16 Massachusetts Gen Hospital Métodos para la detección multiplexada de moléculas diana y sus usos
WO2017075293A1 (en) 2015-10-28 2017-05-04 Silicon Valley Scientific, Inc. Method and apparatus for encoding cellular spatial position information
CN113271400B (zh) 2016-09-16 2023-12-19 索尼半导体解决方案公司 成像装置和电子设备
EP4556575A3 (en) * 2016-11-21 2025-10-08 Bruker Spatial Biology, Inc. A method for sequencing nucleic acids
JP2020523993A (ja) * 2017-06-16 2020-08-13 ザ ジャクソン ラボラトリーThe Jackson Laboratory 単一核および単分子クロマチン相互作用アッセイ
CN109429261A (zh) * 2017-08-31 2019-03-05 索尼公司 用于无线通信的电子设备和方法
WO2020123316A2 (en) * 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
CN110577983A (zh) * 2019-09-29 2019-12-17 中国科学院苏州生物医学工程技术研究所 高通量单细胞转录组与基因突变整合分析方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017019456A2 (en) * 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
EP3541956A1 (en) * 2016-11-17 2019-09-25 Spatial Transcriptomics AB Method for spatial tagging and analysing nucleic acids in a biological specimen
WO2019079399A1 (en) * 2017-10-20 2019-04-25 Duke University DEVICES, SYSTEMS AND METHODS FOR HIGH SPEED SINGLE CELL ANALYSIS

Also Published As

Publication number Publication date
EP4105340A4 (en) 2023-08-09
WO2021161893A1 (ja) 2021-08-19
JPWO2021161893A1 (https=) 2021-08-19
JP7647736B2 (ja) 2025-03-18
EP4105340A1 (en) 2022-12-21
JP2025087797A (ja) 2025-06-10
CN115052995A (zh) 2022-09-13
US20230066282A1 (en) 2023-03-02

Similar Documents

Publication Publication Date Title
US20230313279A1 (en) Methods of determining the location of an analyte in a biological sample using a plurality of wells
CN100485032C (zh) 生物分子基底,使用它的检验和诊断方法及装置
CN115052995B (zh) 分析方法、分析系统及分析表面
CN105026562B (zh) 基因定量、序列分析用二维细胞阵列器件及装置
US20260116065A1 (en) Systems and methods for spatial screening of analytes
CN110079585A (zh) 基因表达解析方法、基因表达解析用设备及基因表达解析装置
JP6307179B2 (ja) 単一細胞解析用フローセルデバイス及び単一細胞解析装置
JP2016052320A (ja) 2次元cDNAライブラリを用いた遺伝子発現解析方法
JP7740398B2 (ja) 粒子確認方法、粒子捕捉用チップ、及び粒子分析システム
US20230174970A1 (en) Spatial multiomics and live biology with continuous imaging
US11835510B2 (en) Obtaining information from a biological sample in a flow cell
JP7816150B2 (ja) 生体粒子分析方法及び生体粒子分析システム
US20180299360A1 (en) Methods for selectively analyzing biological samples
JP2004354164A (ja) 微粒子を用いた検体の検査方法及びその検査システム
KR20190112944A (ko) 생체시료의 선별 분리방법 및 선별 분리장치
CN117015710A (zh) 生物粒子分析方法及用于生物粒子分析的试剂盒
WO2023188896A1 (ja) 生体粒子解析システム、情報処理装置、及び生体粒子解析方法
Gowdavally Biochip Technology–A Gigantic Innovation

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant