CN115047194A - Fluorescent quantitative detection reagent card - Google Patents
Fluorescent quantitative detection reagent card Download PDFInfo
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- CN115047194A CN115047194A CN202210659816.XA CN202210659816A CN115047194A CN 115047194 A CN115047194 A CN 115047194A CN 202210659816 A CN202210659816 A CN 202210659816A CN 115047194 A CN115047194 A CN 115047194A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/165—Coronaviridae, e.g. avian infectious bronchitis virus
Abstract
The utility model relates to a detect reagent card field, specifically disclose a fluorescence quantitative determination reagent card, it includes shell and detection module, detection module includes the bottom plate and sets gradually the sample loading pad on the bottom plate, combine to fill up, detect and fill up and inhale the appearance and fill up, the shell corresponds to sample loading hole has been seted up at sample loading pad's position, the shell corresponds to the observation hole has been seted up at the position of combination pad, in the shell in sample loading pad keeps away from the one end of inhaling the appearance pad is equipped with the cover and locates the windband of sample loading pad end, be equipped with in the shell be used for to the air feed mechanism of air feed in the windband. The application has the effects of high sampling liquid chromatography speed and high detection efficiency, and is suitable for screening the high-flow region in real time.
Description
Technical Field
The application relates to the field of detection reagent cards, in particular to a fluorescent quantitative detection reagent card.
Background
The novel coronavirus IgM antibody detection kit generally comprises the following components according to the action principle: colloidal gold method, fluorescence immunochromatography, enzyme-linked immunosorbent assay, chemiluminescence method and the like. The principle of the fluorescence immunochromatography is based on a dry fluorescence labeling technology and an immunochromatography technology, a sample is dripped on a sample pad, and the sample moves forwards along a detection card under the action of capillary. The novel coronavirus (SARS-CoV-2) antigen in the sample binds to the labeled novel coronavirus (SARS-CoV-2) nucleocapsid protein monoclonal antibody to form an antigen-antibody complex. This complex migrates upward on the membrane by capillary effect until captured by a pre-coated monoclonal antibody to the novel coronavirus (SARS-CoV-2) nucleocapsid protein at the detection line (T-line). As a quality control, the fluoroimmunoassay analyzer recognizes that the C-line signal indicates that the appropriate volume of sample has been added and that membrane chromatography has occurred.
Chinese patent No. CN206804667U in the related art proposes a multi-index time-resolved fluorescence immunochromatographic kit for rapid quantitative detection of renal failure, which comprises a detection card, wherein the detection card comprises a sample loading pad, a binding pad, a detection pad with a detection line T and a quality control line C, a sample suction pad and a bottom plate.
With respect to the related art among the above, the inventors consider that the following drawbacks exist: after the sampling liquid is dripped into a sample adding hole of a reagent card, the sampling liquid permeates into a combination pad loaded with detection antigen in a sample loading pad by virtue of capillary action and then permeates into a detection pad, even if the sample sucking pad is arranged at the other end of the reagent card to promote the chromatography speed of the sampling liquid in the combination pad and the detection pad, the actual detection still needs to consume 10-15 minutes, the detection efficiency is low, and the method is not suitable for timely sampling detection in areas with large human flow; in the process, the combination pad and the sampling pad are both exposed in the air and are easy to be secondarily polluted, so that the detection result is influenced.
Disclosure of Invention
In order to solve the problem that the detection rate is low because the reagent card needs to be static for a long time in self-testing use, the application provides a fluorescence quantitative detection reagent card.
The application provides a fluorescence quantitative determination reagent card adopts the following technical scheme:
the utility model provides a fluorescence quantitative determination reagent card, includes shell and detection module, detection module includes the bottom plate and sets gradually the sample loading pad, combines to fill up, detects the pad and inhale the sample pad on the bottom plate, the shell corresponds to sample loading hole has been seted up at the position that fills up, the shell corresponds to the inspection hole has been seted up at the position that combines the pad, in the shell the sample loading pad is kept away from the one end that inhales the sample pad is equipped with the cover and locates the windband of sample loading pad end, be equipped with in the shell be used for to the air feed mechanism of air feed in the windband.
Through adopting above-mentioned technical scheme, dripping into the back to the last appearance pad from the hole of getting one's sample with more drop number, supply fresh air in to the wind cover through air feed mechanism, this air forms the air current and fills up to inhaling appearance pad circulation by the last appearance in the shell, can make sampling liquid chromatography in last appearance pad, combination pad and detection pad rapider, improved detection efficiency to can reduce the check-out time of this application to a certain extent. The method is particularly applicable to the real-time screening of passenger flows in high-passenger-flow areas needing to be detected on the ground, such as airports, passenger stations, railway stations and the like, has the advantages of quick detection timeliness and short passenger flow stagnation time, and can effectively and timely warn so as to prevent further spread of infection after the passenger flows are dispersed.
Optionally, an air inlet of the air sleeve is provided with an air buffering block, and a plurality of air holes penetrate through the air buffering block.
Through adopting above-mentioned technical scheme, the setting of slow wind piece can make the air current that gets into more mildly, smoothly from the wind cover air inlet to the air current that is close to wind cover air inlet department is too big, blows off the sampling liquid easily, has guaranteed as far as possible that the effective reaction of sampling liquid and combining pad and material in the sampling pad is long.
Optionally, the air supply mechanism includes one end and the inflator of wind cover intercommunication, the inflator inlay card in the shell, the sealed slip is provided with the piston in the inflator, the rigid coupling has one end to extend to on the piston the outside push rod of shell.
By adopting the technical scheme, after the shell is held by hand, the drawing push rod can drive the piston to reciprocate in the inflator, so that the air supply to the air sleeve can be carried out continuously, and the speed of the drawing push rod is controllable, so that the flow velocity of the air flow output by the air sleeve can be controllable.
Optionally, one end of the push rod, which is located outside the housing, is fixedly connected with a first sealing plate;
when the push rod pushes the piston to be closest to the sample loading pad, the first sealing plate completely seals the sample loading hole.
By adopting the technical scheme, when the push rod is pushed, the first sealing plate can be driven to slide on the shell, and when the airflow in the air sleeve blows the sampling liquid detection module for chromatography, more sampling liquid adsorbed at the sample loading pad is blown out from the sample loading hole with a certain probability, so that pollution is caused; and at this moment, because the first closing plate can block the sample loading hole when sliding along with the push rod, the phenomenon can be effectively avoided.
Optionally, the air supply mechanism includes an air bag communicated with the windband, a reset structure for driving the air bag to expand and reset is arranged in the air bag, and a squeezing structure for squeezing the air bag is arranged on the housing.
Through adopting above-mentioned technical scheme, after dripping the appearance and accomplishing, press the air pocket through crowded structure of pushing away for the air output of storing in the air pocket is to the air pocket, stops to press the air pocket after, and the reset structure further drives the air pocket inflation of being shriveled and resumes to the former state, thereby can last the air that supplies in the air pocket, in order to accelerate the chromatography speed of sampling liquid in detection module.
Optionally, the return structure comprises a return spring arranged along the contraction direction of the air bag.
Through adopting above-mentioned technical scheme, the air pocket is by the flat back of pressure, and reset spring is compressed and is produced deformation, presses the back when stopping external force, and reset spring's deformation power orders about the air pocket inflation and resets to continue to suck the air, in order to do benefit to next air feed in to the air jacket.
Optionally, the pushing structure includes a chute disposed on the housing and arranged along a length direction of the housing, a pressing plate located on a side of the air bag away from the sample loading pad is disposed in the housing, one end of the pressing plate penetrates through the chute and extends to an outside of the housing, and a second sealing plate is fixedly connected to the end of the pressing plate;
when the pressing plate slides to be closest to the sample loading pad, the second sealing plate completely seals the sample loading hole.
Through adopting above-mentioned technical scheme, at the in-process through clamp plate extrusion air pocket, the second shrouding is followed the clamp plate and is removed together to carry out the shutoff to the hole of drawing a design, can effectively avoid the windband air feed when too much, the sampling liquid in the pad of drawing a design is blown out and is caused the pollution.
Optionally, the housing is covered with a transparent film at the observation hole.
Through adopting above-mentioned technical scheme, the setting of transparent film can effectively be completely cut off, protect the detection pad of observing hole department to prevent this application from unsealing the back and detecting the pad and arrange the emergence pollution in the air for a long time.
Optionally, the rigid coupling has to enclose to establish in the shell a plurality of backplate of bottom plate week side, works as the shell seals the back, the backplate with the shell encloses to synthesize and is used for holding detection module's airtight chamber.
Through adopting above-mentioned technical scheme, a plurality of backplate and shell inner wall enclose synthetic airtight chamber, can effectively ensure that the air current of output in the wind cover can fill in proper order in the pad of going up the appearance, combination pad and detection pad, promote the chromatography speed of sampling liquid as high as efficient as possible.
Optionally, follow two of shell length direction the equal interval rigid coupling of lateral wall that the backplate is close to mutually has a plurality of fins, the fin is followed shell length direction is the slope setting.
By adopting the technical scheme, when the air flow output by the air sleeve circulates in the closed cavity, the air flow can be guided by the arrangement of the wing plates, and on one hand, the air flow can be gathered to ensure a better blowing effect on the sampling liquid; on the other hand, the two wing plates which are oppositely arranged reduce the sectional area when the airflow passes through, and can play a role in accelerating the airflow.
In summary, the present application includes at least one of the following beneficial technical effects:
1. after the sampling liquid with more number of drips instil into to the last appearance pad from the hole of going up the appearance, supply fresh air in to the wind jacket through air feed mechanism, this air forms the air current and fills up to inhaling the appearance pad circulation by the last appearance in the shell, can make sampling liquid more rapidly in the chromatography of last appearance pad, combination pad and detection pad, has improved detection efficiency to can reduce the check-out time of this application to a certain extent. Particularly, the method can be used for timely screening of passenger flow in some high-passenger-flow areas needing to be detected on the ground, such as airports, passenger stations, railway stations and the like, has quick detection timeliness and small passenger flow stagnation time, and can effectively warn in time so as to prevent further spread of infection after the passenger flow is dispersed;
2. in the process of supplying air into the air sleeve, the first sealing plate and the second sealing plate can dynamically seal the sample loading hole, so that the phenomenon that the sampling liquid in the sample loading pad is blown out to cause pollution when the air sleeve supplies excessive air is effectively avoided;
3. when the air flow output by the air sleeve circulates in the closed cavity, the air flow can be guided by the arrangement of the wing plates, so that on one hand, the air flow can be gathered to ensure a better blowing effect on the sampling liquid; on the other hand, the two wing plates which are oppositely arranged reduce the sectional area when the airflow passes through, and can play a role in accelerating the airflow.
Drawings
Fig. 1 is a schematic view of the overall structure of embodiment 1 of the present application.
Fig. 2 is a schematic structural diagram of a detection module in embodiment 1 of the present application.
FIG. 3 is a schematic sectional structure diagram of application example 1.
FIG. 4 is a schematic sectional structure diagram of application example 2.
Description of reference numerals: 1. a housing; 11. sample loading holes; 12. an observation hole; 13. a transparent film;
20. a base plate; 21. a sample loading pad; 22. a bonding pad; 23. a detection pad; 24. a sample sucking pad;
3. a wind sleeve;
4. a slow wind block;
51. an air cylinder; 52. a piston; 53. a push rod; 54. a first seal plate;
61. an air bag; 62. a return spring; 63. a chute; 64. pressing a plate; 65. a second closing plate; 66. a limiting plate;
71. a guard plate; 72. a closed cavity; 73. an air outlet; 74. a fin.
Detailed Description
The present application is described in further detail below with reference to figures 1-4.
Example 1:
the embodiment of the application discloses a fluorescence quantitative detection reagent card. Referring to fig. 1 and 2, the fluorescence quantitative detection reagent card comprises a shell 1 and a detection module, wherein the shell 1 is formed by buckling a face shell and a bottom shell, the detection module comprises a bottom plate 20, and a sample loading pad 21, a combination pad 22, a detection pad 23 and a sample sucking pad 24 which are sequentially arranged on the bottom plate 20, a sample loading hole 11 is formed in a position of the shell 1 corresponding to the sample loading pad 21, and an observation hole 12 is formed in a position of the shell 1 corresponding to the combination pad 22. The one end of keeping away from in the sample pad 21 of going up in shell 1 and inhaling sample pad 24 is equipped with the wind cover 3 that the sample pad 21 head end was located to the cover, the air intake department of wind cover 3 is provided with slow wind piece 4, slow wind piece 4 is filled in wind cover 3, it has a plurality of bleeder vents to run through in the slow wind piece 4, during concrete setting, slow wind piece 4 can be the sponge, the activated carbon layer, filter pulp etc. makes, can play slow wind effect, can play again and carry out filterable effect to the air, be equipped with the air feed mechanism that is used for air feed in the wind cover 3 in the shell 1.
So set up the back, when carrying out quick self-checking, the person of examining can drop into more sampling liquid of drop number to the back in the sample loading pad 21 from sample loading hole 11, supplies fresh air in to windband 3 through air feed mechanism, and the setting up of slow wind piece 4 enables to obtain that the air current that windband 3 air intake got into is gentler, smooth and easy more. And this air current is by the pad of getting a sample 21 to inhale a sample pad 24 circulation in shell 1, can make sampling liquid go up sample pad 21, combination pad 22 and detect and fill up 23 middle chromatography faster, improved detection efficiency to can reduce the check-out time of this application to a certain extent. The method is particularly applicable to timely screening of passenger flow in high passenger flow areas needing to be detected on the ground, such as airports, passenger stations, railway stations and the like, has the advantages of quick detection timeliness and short passenger flow dead time, and can effectively and timely warn so as to prevent further spread of infection after passenger flow dispersion.
Considering that the detection reagent card of the present application is a disposable product, and considering the processing cost and the assembly portability, referring to fig. 3, the air supply mechanism is configured to include an air cylinder 51 having one end communicating with the air jacket 3, the air cylinder 51 is embedded in the housing 1 and arranged along the length direction of the housing 1, a piston 52 is arranged in the air cylinder 51 in a sealing and sliding manner, and a push rod 53 having one end extending to the outside of the housing 1 is fixedly connected to the piston 52; the specific design is that two baffles are integrally formed on the bottom shell of the shell 1, two ends of the inflator 51 in the length direction are clamped and embedded between the two baffles, a notch for inserting the push rod 53 is formed in the baffle departing from the sample loading pad 21, and a hole for the push rod 53 to generate is reserved at the end part of the shell 1. The end of the push rod 53 outside the housing 1 is fixedly connected with a first sealing plate 54, the first sealing plate 54 and the air cylinder 51 are integrally formed in a C shape, and when the push rod 53 pushes the piston 52 to be closest to the loading pad 21, the first sealing plate 54 completely seals the loading hole 11.
Thus, when assembling the present application, the piston 52 and the push rod 53 are assembled with the air cylinder 51, the assembled air cylinder 51 is clamped between the two baffles on the bottom shell of the housing 1, the face shell of the housing 1 is covered, and the first sealing plate 54 is attached to the surface of the housing 1.
When the air supply device is used, after the shell 1 is held by hand, the drawing push rod 53 can drive the piston 52 to reciprocate in the air cylinder 51, so that the air sleeve 3 can be continuously supplied with air, and the speed of the drawing push rod 53 is controllable, so that the flow rate of air flow output by the air sleeve 3 can be controlled; simultaneously when promoting push rod 53, can drive first shrouding 54 and slide on shell 1 to even more sampling liquid that sample pad 21 department was adsorbed is blown out by the air current from sample hole 11 department, first shrouding 54 also can carry out the shutoff to sample hole 11, has effectively avoided secondary pollution.
Also to reduce secondary contamination during use of the present application, referring to fig. 3, the housing 1 is covered with a transparent film 13 at the viewing aperture 12. The transparent film 13 can effectively isolate and protect the detection pad 23 at the observation hole 12, so as to prevent the detection pad 23 from being polluted when being placed in the air for a long time after the application is opened.
Furthermore, during actual setting, the width of the bottom plate 20 does not reach the width of the housing 1, that is, the airflow output by the windband 3 escapes into the inner cavity of the housing 1 with high probability, so that the promotion effect of the chromatographic speed of the sampling solution in the detection module is weak, especially the detection pad 23 far away from the sample loading pad 21, and the effect of improving the detection speed is not obvious.
In view of the above, referring to fig. 3, a plurality of guard plates 71 surrounding the periphery of the bottom plate 20 are fixedly connected in the housing 1, specifically, the guard plates 71 are integrally formed on the bottom shell of the housing 1, when the housing 1 is closed, the guard plates 71 and the housing 1 surround to form a sealed cavity 72 for accommodating the detection module, and an air outlet hole 73 for communicating with the sealed cavity 72 is reserved at one end of the housing 1 away from the sample loading hole 11; the lateral wall that two backplate 71 along shell 1 length direction are close to mutually all has the interval rigid coupling to have a plurality of fins 74, and a plurality of fins 74 on two backplate 71 are the one-to-one setting, and fin 74 is the slope setting along shell 1 length direction.
Therefore, the airflow output by the windband 3 enters the closed cavity 72 formed by the plurality of guard plates 71 and the inner wall of the shell 1, the airflow output by the windband 3 can be effectively ensured to be sequentially filled in the sample loading pad 21, the combination pad 22 and the detection pad 23 and finally discharged from the air outlet 73, and the chromatography speed of the sampling solution can be promoted as efficiently as possible; in the process of circulating the airflow in the closed cavity 72, the arrangement of the wing plates can guide the airflow, so that on one hand, the airflow can be gathered to ensure a better blowing effect on the sampling liquid; on the other hand, the two wing plates which are oppositely arranged reduce the sectional area of the airflow when the airflow passes through the two wing plates, a Venturi effect is formed, the effect of accelerating the airflow can be achieved, the chromatography speed of the airflow in the detection module after the sampling liquid is wrapped by the airflow can be promoted, and the detection speed is further improved.
The application principle of the fluorescent quantitative detection reagent card is as follows: when carrying out quick self-checking, the back in the sample pad 21 is dripped into to the sample hole 11 with more sampling liquid of drop number by the examinee, and pull push rod 53 can drive piston 52 reciprocating motion in inflator 51, and then can carry out the continuous air feed to wind cover 3, and the setting of slow-breeze piece 4 enables to obtain that the air current that the air intake of wind cover 3 got into is gentler, smooth and easy more. And this air current is by the pad of getting a sample 21 to inhale a sample pad 24 circulation in shell 1, can make sampling liquid go up sample pad 21, combination pad 22 and detect and fill up 23 middle chromatography faster, improved detection efficiency to can reduce the check-out time of this application to a certain extent. The method is particularly applicable to timely screening of passenger flow in high passenger flow areas needing to be detected on the ground, such as airports, passenger stations, railway stations and the like, has the advantages of quick detection timeliness and short passenger flow dead time, and can effectively and timely warn so as to prevent further spread of infection after passenger flow dispersion.
Example 2:
the embodiment of the application discloses a fluorescence quantitative detection reagent card. Referring to fig. 4, the difference from embodiment 1 is that: the air supply mechanism comprises an air bag 61 communicated with the windband 3, a reset structure for driving the air bag 61 to expand and reset is arranged in the air bag 61, and the reset structure comprises a reset spring 62 which is arranged in the air bag 61 and arranged along the contraction direction of the air bag 61; when the air bag 61 is specifically arranged, the air bag is cylindrical, two parallel limiting plates 66 are fixedly connected to the bottom shell of the shell 1, and the limiting plates 66 are arranged along the axial direction of the air bag 61. The casing 1 is provided with a squeezing structure for conveniently squeezing the air bag 61, the squeezing structure comprises a sliding groove 63 which is arranged on the casing 1 and arranged along the length direction of the casing 1, a pressing plate 64 which is positioned at one side of the air bag 61 far away from the sample loading pad 21 is arranged in the casing 1, one end of the pressing plate 64 penetrates through the sliding groove 63 and extends to the outside of the casing 1, and a second sealing plate 65 is fixedly connected to the end part; when the pressing plate 64 slides to be closest to the loading pad 21, the second closing plate 65 completely closes the loading hole 11.
Accordingly, after the dropping sample is completed, the pressing plate 64 is pushed to press the air bag 61, so that the air stored in the air bag 61 is output to the windband 3, and after the pressing of the air bag 61 is stopped, the deformation force of the return spring 62 drives the deflated air bag 61 to expand and return to the original shape, so that the air can be continuously supplied into the windband 3 by repeatedly pushing the pressing plate 64, and the chromatography speed of the sampling solution in the detection module is increased. In the process, the second sealing plate 65 arranged in the same way can also effectively avoid the phenomenon that the sampling liquid in the sampling pad 21 is blown out to cause pollution when the air supply of the air sleeve 3 is excessive.
The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (10)
1. The utility model provides a fluorescence quantitative determination reagent card, includes shell (1) and detection module, detection module includes bottom plate (20) and sets gradually last appearance pad (21), combination pad (22), detection pad (23) and inhale appearance pad (24) on bottom plate (20), shell (1) is corresponding to sample hole (11) have been seted up at the position of last appearance pad (21), shell (1) corresponds observation hole (12), its characterized in that have been seted up at the position of combination pad (22): the utility model discloses a sample suction device, including shell (1), in sample feeding pad (21) keep away from in shell (1) the one end of inhaling sample pad (24) is equipped with the cover and locates wind cover (3) of sample feeding pad (21) head end, be equipped with in shell (1) be used for to the air feed mechanism of air feed in wind cover (3).
2. The reagent card for fluorescent quantitative detection of claim 1, wherein: the air inlet of the air sleeve (3) is provided with a slow air block (4), and a plurality of air holes penetrate through the slow air block (4).
3. The reagent card for fluorescent quantitative detection according to claim 2, wherein: the air feed mechanism includes one end with inflator (51) of windband (3) intercommunication, inflator (51) inlay card in shell (1), inflator (51) internal seal slides and is provided with piston (52), the rigid coupling has one end to extend to push rod (53) of shell (1) outside on piston (52).
4. The reagent card for fluorescent quantitative detection according to claim 3, wherein: one end of the push rod (53) positioned outside the shell (1) is fixedly connected with a first sealing plate (54);
when the push rod (53) pushes the piston (52) to be closest to the loading pad (21), the first closing plate (54) completely closes the loading hole (11).
5. The reagent card for fluorescent quantitative detection according to claim 2, wherein: the air supply mechanism comprises an air bag (61) communicated with the air sleeve (3), a reset structure used for driving the air bag (61) to expand and reset is arranged in the air bag (61), and a squeezing structure used for squeezing the air bag (61) is arranged on the shell (1).
6. The reagent card for fluorescent quantitative detection of claim 5, wherein: the return structure includes a return spring (62) provided in a contraction direction of the air bag (61).
7. The reagent card for fluorescent quantitative detection of claim 6, wherein: the squeezing and pushing structure comprises a sliding groove (63) which is arranged on the shell (1) and arranged along the length direction of the shell (1), a pressing plate (64) which is positioned on one side of the air bag (61) far away from the sample loading pad (21) is arranged in the shell (1), one end of the pressing plate (64) penetrates through the sliding groove (63) and extends to the outside of the shell (1), and a second sealing plate (65) is fixedly connected to the end part of the pressing plate (64);
when the pressing plate (64) slides to be closest to the loading pad (21), the second sealing plate (65) completely seals the loading hole (11).
8. The reagent card for fluorescent quantitative detection according to any one of claims 1 to 7, wherein: the shell (1) is covered with a transparent film (13) at the observation hole (12).
9. The reagent card for fluorescent quantitative detection of claim 8, wherein: rigid coupling has to enclose in shell (1) and establishes a plurality of backplate (71) of bottom plate (20) week side work as shell (1) seals the back, backplate (71) with shell (1) encloses to synthesize and is used for holding detection module's airtight chamber (72).
10. The reagent card for fluorescent quantitative detection of claim 9, wherein: follow shell (1) length direction's two the equal interval rigid coupling of lateral wall that backplate (71) are close to mutually has a plurality of fin (74), fin (74) are followed shell (1) length direction is the slope setting.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210659816.XA CN115047194B (en) | 2022-06-13 | 2022-06-13 | Fluorescent quantitative detection reagent card |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210659816.XA CN115047194B (en) | 2022-06-13 | 2022-06-13 | Fluorescent quantitative detection reagent card |
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| CN115047194A true CN115047194A (en) | 2022-09-13 |
| CN115047194B CN115047194B (en) | 2023-06-30 |
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