CN115040468A - A Chinese medicinal composition for oral administration or administration of spicy food for middle-sized animals, and its preparation method - Google Patents

A Chinese medicinal composition for oral administration or administration of spicy food for middle-sized animals, and its preparation method Download PDF

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CN115040468A
CN115040468A CN202210529516.XA CN202210529516A CN115040468A CN 115040468 A CN115040468 A CN 115040468A CN 202210529516 A CN202210529516 A CN 202210529516A CN 115040468 A CN115040468 A CN 115040468A
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administration
oral immunization
inner core
spicy
oral
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CN115040468B (en
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沈青春
蒋卉
丁家波
范学政
张广智
李松励
秦彤
梁琳
汤新明
梁瑞英
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Institute of Animal Science of CAAS
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
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    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
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    • A—HUMAN NECESSITIES
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    • A61K2039/552—Veterinary vaccine
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Abstract

The invention discloses a spicy strip for oral administration or administration of medium and large animals, which comprises an inner core and an external wrapping layer, wherein the inner core is an edible soft substance capable of absorbing liquid; the outer wrapping layer comprises a phagostimulant; the oral immunization or administration of the spicy strips for medium and large animals disclosed by the invention has inductivity on the oral immunization or administration of large and medium animals, enhances the initiative of animal feeding, changes the oral immunization or administration mode from forced animal fixation and forced drenching into the initiative of animal feeding, reduces the stress reaction of animals, improves the safety of feeding personnel, and greatly lightens the labor intensity of operating personnel; the drug delivery system can be applied to the drug delivery of various drugs or vaccines, and has good universality; the risk of people and livestock suffering from brucellosis and the like due to contact with animals is reduced, and the safety is good; the production method is simple and easy to operate, mild in condition, free of potential safety hazard and environmental pollution, and beneficial to industrial wide popularization and application.

Description

A Chinese medicinal composition for oral administration or administration of spicy food for middle-sized animals, and its preparation method
Technical Field
The invention relates to the technical field of medicines, in particular to a spicy strip for oral immunization or administration of medium and large animals and a production method thereof.
Background
In the process of breeding medium and large animals, epidemic disease prevention and treatment of various animal diseases need to be carried out, the animals are mainly administrated by injection and oral administration at present, and due to the problems of injection pain, poor palatability of oral liquid and the like, the animals are not matched and can only be completed by forced restraint, forced drenching or injection. Animals such as pigs, sheep and the like need at least 2 people for baoding, and large animals such as cows, horses, camels and the like need more operation and guarantee personnel; the administration mode has low efficacy and high labor intensity of personnel, is easy to cause animal stress reaction, and can also cause injury to personnel due to carelessness; in some animal populations with zoonosis, infections of the operator may also result during mandatory security procedures.
The related technical scheme disclosed by the prior art comprises an administration device and an administration device which are applied to animals, and medicines are made into feeding-inducing pills, paste, capsules and the like; in the actual use process, the drug delivery device or the drug delivery device is not suitable for large animals and is easy to swallow by the large animals; the attractant pills, the ointment and the capsules have poor attraction effect on large animals, poor administration effect in use and high proportion of medicine leakage; in addition, the existing administration devices, administration apparatuses, feeding-inducing pills, ointments and capsules can only administer one medicament; the drug can not be administered to various drugs, and the universality is poor.
Accordingly, those skilled in the art have endeavored to develop a "spicy strip" for oral immunization or administration to medium-sized animals and a method for producing the same, which addresses the deficiencies of the prior art described above.
Disclosure of Invention
In view of the above defects of the prior art, the technical problems to be solved by the invention are that the efficiency is low and the labor intensity of operators is high when the drug is administered to medium and large animals disclosed in the prior art; can not be applied to the administration of various medicines, and has poor application universality.
In order to achieve the above objects, the present invention provides in a first aspect an oral immunization or administration "pepperstrip" for medium and large animals, comprising an inner core and an outer wrapping layer, wherein the inner core is an edible soft substance capable of absorbing liquid; the outer wrapping layer comprises a phagostimulant;
further, the inner core is made from one or more of flour, white sugar, sucrose, fructose, tryptone powder, gelatin, egg white, agar and baking soda;
further, the external wrapping layer is made of crispy colloid or bread material;
furthermore, the external wrapping layer is made of edible glue, flour, milk, egg white or edible polymer materials, a certain amount of phagostimulant is added, and the phagostimulant with a certain concentration can also be brushed on the surface of the wrapping layer;
further, the phagostimulant is one or more of a taste enhancer, grass flavor, sweet orange flavor, essence, enzyme, pyrophosphate, sodium glutamate, salt and the like; or the phagostimulant is a commercialized animal phagostimulant or a flavoring agent;
further, the percentage content of the phagostimulant in the external wrapping layer is 0.001% -5%; preferably, the percentage content is 1 to 5 percent
Further, the outer surface of the inner core also comprises an egg white or agar layer;
further, the outer wrapping layer is made of edible glue or edible high polymer materials;
further, the oral immunization or administration "spicy strip" is one of a strip shape, an oval shape, a cylindrical shape, a square shape, a spherical shape and an irregular shape;
further, the length of the oral immunization or administration spicy strip is 5-15 cm, and the diameter of the oral immunization or administration spicy strip is 1.5-6 cm;
preferably, the length of the oral immunization or administration 'spicy strip' is 9-11 cm, and the diameter is 2-4 cm;
further, the medium and large animals are horses, cattle, yaks, camels, pigs, sheep and deer;
in a specific embodiment of the invention, the outer surface of the inner core further comprises an egg white layer;
in another embodiment of the present invention, the outer surface of the carrier core further comprises an agar layer;
in a particular embodiment of the invention, the length of the oral immunization or administration "peppery strip" is 6 cm;
in another embodiment of the invention, the length of said oral immunization or administration "peppers" is 8 cm;
in another embodiment of the invention, the length of said oral immunization or administration "peppers" is 10 cm;
in a particular embodiment of the invention, the diameter of the oral immunization or administration "peppery strip" is 2.5 cm;
in another embodiment of the invention, said oral immunization or administration "peppers" have a diameter of 3 cm;
in another embodiment of the invention, the oral immunization or administration "peppers" have a diameter of 3.5 cm;
in a specific embodiment of the invention, the percentage content of the phagostimulant in the outer coating is 1%;
in another specific embodiment of the invention, the percentage content of the phagostimulant in the outer coating is 3%;
in another embodiment of the invention, the percentage of the phagostimulant in the outer coating is 5%;
in a specific embodiment of the invention, the medium-sized animals are immunized or administered with "peppers" orally, and the inner core is edible soft material capable of absorbing liquid and has the functions of absorbing the medicine and keeping the stability of the medicine;
in a specific embodiment of the invention, the medium-sized animals are orally immunized or administered with the "peppers", and the outer wrapping layer is an edible coat or bread outer layer containing a phagostimulant, which mainly functions to prevent medicine leakage and enhance the initiative of the animals to eat the "peppers";
in a second aspect, the invention provides a method for producing a "spicy strip" for oral immunization or administration to medium and large animals, comprising the following steps:
step 1, manufacturing an inner core: mixing the manufacturing raw materials of the inner core according to a certain proportion, adding water to prepare dough, and shaping the dough into a shape with the length of 3-6 cm and the diameter of about 1.5-3 cm; then coating egg white or agar on the surface, and steaming and baking to obtain a soft inner core with certain toughness;
step 2, wrapping the soft inner core obtained in the step 1 with an external wrapping layer added with a phagostimulant, and baking and forming again to obtain the oral immunization or administration spicy strips;
step 3, carrying out single plastic packaging on the oral immunization or administration spicy strips obtained in the step 2 by using a food-grade film bag, and filling inert gas into the packaging to protect the oral immunization or administration spicy strips;
further, in the step 1, raw materials for manufacturing the inner core are flour, sugar, protein and baking soda;
further, the weight ratio (g: g) of the flour, the saccharides, the protein and the baking soda is (10-80): (5-20): 20-50): 1;
further, in the step 1, the volume-to-weight ratio (mL: g) of the added water to the total amount of the raw materials for manufacturing the inner core is 1: 1-1: 1.8;
further, in the step 1, the steaming and baking temperature is 95-105 ℃;
further, in the step 2, the weight ratio (g: g) of the external wrapping layer to the inner core is 1: 1-3: 1;
further, in the step 2, the baking temperature is 120-220 ℃;
further, in the step 3, the inert gas is nitrogen or helium;
in a specific embodiment of the present invention, in the step 1, the baking is performed in an oven or an oven;
in a specific embodiment of the invention, in the step 1, the weight ratio (g: g) between the flour, the sugar, the protein and the baking soda is 20:5:20: 1;
in another specific embodiment of the present invention, in the step 1, the weight ratio (g: g) between the flour, the sugar, the protein and the baking soda is 30:10:30: 1;
in another specific embodiment of the present invention, in the step 1, the weight ratio (g: g) between the flour, the sugar, the protein and the baking soda is 50:20:50: 1;
in a specific embodiment of the invention, in the step 1, the volume-to-weight ratio (mL: g) of the added water to the total amount of the raw materials for making the inner core is 1: 1;
in another specific embodiment of the invention, in the step 1, the volume-to-weight ratio (mL: g) of the added water to the total amount of the raw materials for making the inner core is 1: 1.2;
in another specific embodiment of the invention, in the step 1, the volume-to-weight ratio (mL: g) of the added water to the total amount of the raw materials for making the inner core is 1: 1.5;
in a specific embodiment of the present invention, in the step 1, the temperature of the steaming and baking is 95 ℃;
in another specific embodiment of the present invention, in the step 1, the temperature of the steaming is 100 ℃;
in another specific embodiment of the present invention, in the step 1, the temperature of the steaming is 105 ℃;
in a specific embodiment of the invention, in the step 2, the weight ratio (g: g) of the outer coating layer to the inner core is 1: 1;
in another specific embodiment of the present invention, in the step 2, the weight ratio (g: g) of the outer coating layer to the inner core is 2: 1;
in another specific embodiment of the present invention, in the step 2, the weight ratio (g: g) of the outer coating layer to the inner core is 3: 1;
in a specific embodiment of the present invention, in the step 2, the baking temperature is 120 ℃;
in another specific embodiment of the present invention, in the step 2, the baking temperature is 170 ℃;
in another specific embodiment of the present invention, in the step 2, the baking temperature is 220 ℃;
in a specific embodiment of the present invention, the step 1 is specifically operated as follows: mixing flour, saccharides, protein and baking soda according to a certain proportion, adding water to prepare dough, and shaping the dough into a shape with the length of 3-6 cm and the diameter of 1.5-3 cm; then coating egg white or agar on the surface, and steaming and baking at 95-105 ℃ to obtain a soft inner core with certain toughness;
in a specific embodiment of the present invention, the step 2 specifically operates as follows: wrapping a wrapping layer added with a phagostimulant outside the soft inner core obtained in the step 1 according to the weight ratio of 1: 1-1: 3, and baking and forming at 120-220 ℃ to obtain the oral immunization or administration 'spicy strips';
the invention also provides the use of the oral immunization or administration of "peppers" according to any one of the first aspect of the invention in the preparation of a medicament for oral immunization or administration to a large or medium-sized animal; the administration is of a common therapeutic agent; the oral immunization is vaccine oral immunization;
by adopting the scheme, the oral immunization or administration 'spicy strip' for medium and large animals and the production method thereof disclosed by the invention have the following advantages:
(1) the oral immunization or administration 'spicy strip' for medium and large animals is made of edible materials or high polymer materials with good animal palatability, the interior of the spicy strip is made of materials capable of absorbing moisture and protecting liquid medicine, enough liquid medicine can be absorbed, and the drug effect or the immune effect of the spicy strip can not be reduced within hours; the outer layer is wrapped by the edible colloid or bread outer layer, so that the liquid medicine can be prevented from overflowing before feeding, and a phagostimulant or flavoring agent aiming at the target animal is added, so that the initiative of animal feeding is greatly enhanced; when the compound premix is used for animal administration, firstly, the medicine or the vaccine is diluted into 0.2 ml/head part or 0.5 ml/head part or 1.0 ml/head part, and the diluted medicine or the vaccine is injected and added into the spicy strips, so that each spicy strip contains 1 head part of the medicine or the vaccine, the administration work is easily completed in a way of feeding animals one by one, the compound premix has inducibility to oral immunity or administration of large and medium animals, and the labor intensity of operators is greatly reduced;
(2) the oral immunization or administration 'spicy strip' for medium and large animals can be used for administration by injecting various different animal medicines into the soft carrier inner core before use according to the required administration types; the universality is good, and the wide popularization and application are facilitated;
(3) the oral immunization or administration of the spicy strips for medium and large animals greatly reduces the investment of epidemic prevention personnel in the feeding process, reduces the direct contact between the personnel and the animals, reduces the risk of various diseases of the animals, can also reduce the risk of the common diseases of the people and livestock such as brucellosis infected by the contact of the personnel with the animals, and has good safety;
(4) the production method of the oral immunization or administration of the spicy strips for medium and large animals has mild conditions, simple and convenient operation, no three wastes and contribution to industrial popularization and application;
in conclusion, the oral immunization or administration of the spicy strips for medium and large animals disclosed by the invention has inductivity on the oral immunization or administration of the large and medium animals, enhances the initiative of animal feeding, and the oral immunization (or administration) mode is changed from forced feeding after forcibly fixing the animals into the initiative feeding of the animals, so that the stress response of the animals is reduced, the safety of raising personnel is improved, and the labor intensity of operators is greatly reduced; the drug delivery system can be applied to the drug delivery of various drugs or vaccines, and has good universality; the risk of people and livestock suffering from brucellosis and the like due to contact with animals is reduced, and the safety is good; the production method is simple and easy to operate, mild in condition, free of potential safety hazard and environmental pollution, and beneficial to industrial wide popularization and application.
The conception, the specific technical solutions and the technical effects produced by the present invention will be further described with reference to the following detailed description so as to fully understand the objects, the features and the effects of the present invention.
Detailed Description
The following describes several preferred embodiments of the present invention to make the technical contents thereof clearer and easier to understand. The invention may be embodied in many different forms of embodiments, which are intended to be illustrative only, and the scope of the invention is not intended to be limited to the embodiments shown herein.
If there is an experimental method in which specific conditions are not specified, it is usually carried out according to conventional conditions such as the relevant instructions or manuals.
Example 1 preparation of oral immunization "spicy strips" of live vaccine against Brucella melitensis
Step 1, mixing 20g of flour, 15g of cane sugar, 8g of egg white, 13g of tryptone, 3g of gelatin, 4g of milk powder and 1g of baking soda, adding 45mL of water, stirring to prepare dough, and shaping into long shapes with the length of about 3cm and the diameter of about 1.5 cm; then coating 2g of egg white on the surface, and steaming and baking at 95-105 ℃ to obtain 3 soft inner cores with certain toughness, wherein the length of the soft inner core is about 5cm, and the diameter of the soft inner core is about 2 cm;
step 2, coating a layer of edible gum 15g added with 0.15g of green grass fragrance outside the 1 soft inner cores obtained in the step 1 to serve as an external coating layer, and baking and forming at 120 ℃ to obtain the oral immune spicy strips;
step 3, carrying out single plastic packaging on the oral immune spicy strips obtained in the step 2 by using food-grade transparent or semitransparent film bags, and filling nitrogen gas into the packages to protect the oral immune spicy strips; the two ends of the packaging bag are cut in a zigzag manner, so that the packaging bag is convenient to tear when in use;
the oral immune 'spicy strip' inner core is suitable for the survival of Brucella melitensis at room temperature and is used for carrying out oral immunity of Brucella melitensis live vaccine on sheep.
Example 2 preparation of live vaccine oral immunization of Brucella bovis "spicy strips
Step 1, mixing 30g of flour, 10g of fructose, 11g of egg white, 5g of tryptone, 5g of gelatin, 5g of milk powder and 1g of baking soda, adding 65mL of water, stirring to prepare dough, and shaping into long shapes with the length of about 4cm and the diameter of about 2 cm; then coating 4g of egg white on the surface, and steaming and baking at 95-105 ℃ to obtain 4 soft inner cores with certain toughness, wherein the length of each soft inner core is about 8cm, and the diameter of each soft inner core is about 3 cm;
step 2, wrapping 35g of edible gum added with 1g of enzyme outside the 1 soft inner cores obtained in the step 1 to serve as an external wrapping layer, and baking and forming at 170 ℃ to obtain the oral immune spicy strips;
step 3, carrying out single plastic packaging on the oral immune spicy strips obtained in the step 2 by using food-grade transparent or semitransparent film bags, and filling nitrogen gas into the packages to protect the oral immune spicy strips; the two ends of the packaging bag are cut in a zigzag manner, so that the packaging bag is convenient to tear when in use;
the oral immune 'spicy strip' inner core is suitable for survival of Brucella at room temperature, and is used for oral immunization of live Brucella vaccine for cattle.
Example 3 preparation of Camel brucellosis live vaccine oral immunization "spicy stick
Step 1, mixing 50g of flour, 20g of cane sugar, 22g of egg white, 7g of tryptone, 7g of gelatin, 6g of milk powder and 1g of baking soda, adding 100mL of water, stirring to prepare dough, and shaping into long shapes with the length of about 6cm and the diameter of about 2.2 cm; then 8g of egg white is coated on the surface of the egg white, and 6 soft inner cores with certain toughness are obtained after the egg white is steamed and baked at 100 ℃, so that the length of each soft inner core is about 10cm, and the diameter of each soft inner core is about 3.5 cm;
step 2, coating 60g of edible glue added with 3g of sodium glutamate outside the 1 soft inner cores obtained in the step 1 to serve as an external coating layer, and baking and forming at 220 ℃ to obtain the oral immune spicy strips;
step 3, carrying out single plastic package on the oral immune spicy strips obtained in the step 2 by using food-grade transparent or semitransparent film bags, and filling helium gas into the packages to protect the oral immune spicy strips; the two ends of the packaging bag are cut in a zigzag manner, so that the packaging bag is convenient to tear when in use;
the oral immune 'spicy stripe' inner core is suitable for the survival of brucella of camel species at room temperature, and is used for carrying out oral immunity of brucellosis live vaccines on camels.
The oral immune "spicy strips" obtained in the above examples 1 to 3 were subjected to palatability examination:
before feeding in the morning, randomly selecting 10 sheep (or 10 cattle or 10 camels) as a test animal group, extending the oral immune 'spicy strips' of the embodiment 1-3 to the front of the mouth of the corresponding test animal group for about 5cm, and calculating the number of animals in the to-be-tested animal group which is actively fed; repeating the three times of examination, wherein the number of the animals to be detected in each group actively feed is not less than 8; the oral immune spicy strips obtained in the embodiments 1-3 have good palatability, so that animals can realize active feeding; if the number of the healthy sheep (or cattle or camel) is less than 8, randomly selecting 10 (or heads) again to test, and if the number of the healthy sheep (or cattle or camel) is not less than 8 (or heads), judging that the palatability is in accordance with the specification, if the number of the healthy sheep (or cattle or camel) is still less than 8 (or heads), judging that the palatability is not in accordance with the specification;
test example 4 oral immunoprotective potential test
The oral immunization of the live vaccine of brucellosis obtained in example 1, example 2 and example 3 is used for carrying out the experiment of the spicy strip;
firstly, checking that the external package of the oral immunization spicy strip of the brucellosis live vaccine is not damaged, the internal package of the oral immunization spicy strip is free from foreign matters, the length of the oral immunization spicy strip accords with the designed length, and the external wrapping layer covers the whole spicy strip;
diluting the live Brucella vaccine into 1 part/0.5 ml by using phosphate buffer solution (PBS, pH 7.2, 0.02mol/l) containing 0.01% phenol red, injecting the diluted live Brucella vaccine into the middle part of a spicy strip from one end of the spicy strip by using a sterile injector, placing the spicy strip at room temperature (25 +/-2 ℃) for 2 hours, cutting the middle part of the spicy strip, taking out the red dyed part, adding the red dyed part into 1ml PBS to be fully and uniformly smashed, counting live bacteria, taking live bacteria counting of each part of live bacteria counting of the live Brucella vaccine as a control, and testing that the live bacteria count in the spicy strip is not less than 90% of the live bacteria count in a control sample; the oral administration immunity hot strip of the brucellosis live vaccine obtained in the embodiment can be used for ensuring the effectiveness of the brucellosis live vaccine injected into the oral administration immunity hot strip, so that the immunity effectiveness is not reduced, and the oral administration immunity requirement of animals is met;
test example 5 test for protective ability of drug administration
The oral immune "spicy strip" obtained in example 1, example 2 and example 3 was used for the administration protection ability test;
firstly, checking that the external package of the spicy strips is not damaged and the internal package of the spicy strips is free from foreign matters, wherein the length of the spicy strips conforms to the designed length, and the external wrapping layer covers the whole spicy strips;
diluting levamisole hydrochloride for expelling parasites for cattle into 1 part/ml by using normal saline containing 0.01 percent of phenol red, injecting the levamisole hydrochloride into the middle part of a spicy strip from one end of the spicy strip by using a sterile injector, placing the spicy strip at room temperature (25 +/-2 ℃) for 2 hours, cutting the middle part of the spicy strip, taking out a part impregnated by a medicament, adding the part into the normal saline, fully and uniformly mashing the mixture, carrying out medicament detection, and detecting that the total amount of levamisole hydrochloride in the spicy strip has no obvious change and the effective medicament content is higher than 90 percent of that in an original medicament preparation; the 'spicy strips' obtained in the embodiment can effectively protect the levamisole hydrochloride injected into the spicy strips, so that the drug effect is not reduced, and the treatment and administration requirements of animals are met.
Application examples 6,
The sheep were immunized orally with live vaccine of brucellosis (S2 strain) using the oral immunization "peppers" obtained in example 1:
diluting the brucellosis live vaccine (S2 strain) to 1.2 parts per 0.5ml by using normal saline; the possible loss or injection error is considered, and the immune dose of each sheep is ensured to reach 1 head;
sucking the diluted live vaccine of the brucellosis of the sheep by using an injector, directly penetrating through an outer package, pricking into the middle part of the spicy strip from the central position of one end of the spicy strip, and injecting 0.5ml of vaccine into the spicy strip, so that the preparation of all the spicy strips is completed repeatedly according to the number of sheep to be immunized;
opening the packaging bags of the spicy strips containing the vaccines one by one, feeding the spicy strips to the sheep to be stocked (to be fed) one by one, and ensuring that the vaccine oral immunization of each sheep is completed.
Through practical use, when the oral immune spicy strips of the example 1 are adopted to carry out oral immune on sheep; the operator only needs to stretch the opened oral immune spicy strips which are injected with the vaccine to the position near the mouth of the sheep, and the sheep actively takes food to complete the immune work; the feeding time of each sheep is less than 0.5 minute, and the time and the labor of operators are saved.
Comparative examples 7,
Dividing the cattle into 2 groups, each group having 10 heads; one group of cattle is subjected to brucellosis live vaccine oral immunization by adopting the oral immunization 'spicy strips' of the embodiment 2 by adopting the operation of the application example 6; the other group adopts the prior conventional method to carry out artificial drenching immunization of the live vaccine of the brucellosis;
the oral immune spicy strips in the embodiment 2 are adopted to carry out the oral immune cattle group, the cattle are fed actively one by one quietly and orderly, and the whole immune operation is completed by one operator in 5 minutes;
manually drenching the immunized cattle group by a conventional method, wherein 2 cattle are subjected to baoding, and 1 cattle are drenched; cattle have obvious struggle and howl; the whole immunization operation of the whole group of 10 cattle is completed by 3 persons within 30 minutes; wherein, the cattle hurt the cattle, and the liquid medicine leaks in the process of filling.
The comparative example description and analysis shows that compared with the conventional drenching immunization, the oral immunization 'spicy strip' of the embodiment 2 is adopted to carry out immunization operation on the cattle, and the animal immunization mode is changed from forced animal retention and forced drenching into the active animal feeding, so that the stress reaction of the animals is reduced, the safety of the breeders is improved, the immunization operation period is shortened, the immunization operation personnel are reduced, and the labor intensity of the operators is greatly reduced;
the oral immune 'spicy strip' obtained by other embodiments of the invention has similar beneficial effects as above;
oral immunization or administration of "peppers" according to other embodiments of the present invention have similar beneficial effects as described above.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims (10)

1. The oral immunization or administration spicy strip for medium and large animals is characterized by comprising an inner core and an outer wrapping layer, wherein the inner core is an edible soft substance capable of absorbing liquid; the outer coating layer comprises a phagostimulant.
2. The oral immunization or administration of "peppers" of claim 1, wherein,
the inner core is made from one or more of flour, white sugar, sucrose, fructose, tryptone powder, gelatin, egg white, agar and baking soda;
the external wrapping layer is made of crispy colloid or bread material.
3. The oral immunization or administration of "peppers" of claim 1, wherein,
the phagostimulant is one or more of a taste enhancer, green grass flavor, sweet orange flavor, essence, enzyme, pyrophosphate, sodium glutamate, salt and the like;
the external wrapping layer is made of edible glue, flour, milk, egg white or edible high polymer materials.
4. The oral immunization or administration of "peppers" of claim 1, wherein,
the percentage content of the phagostimulant in the external wrapping layer is 0.001-5%.
5. The oral immunization or administration of "peppers" of claim 1, wherein,
the oral immunization or administration 'spicy strip' is one of a strip shape, an oval shape, a cylindrical shape, a square shape, a spherical shape and an irregular shape;
the length of the oral immunization or administration spicy strip is 5-15 cm, and the diameter of the oral immunization or administration spicy strip is 1.5-6 cm.
6. A production method of orally-administered immunization or administration of spicy sticks for medium and large animals is characterized by comprising the following steps:
step 1, manufacturing an inner core: mixing the manufacturing raw materials of the inner core according to a certain proportion, adding water to prepare dough, and shaping the dough into a shape with the length of 5-6 cm and the diameter of about 1.5-2 cm; then coating egg white or agar on the surface, and steaming and baking to obtain a soft inner core with certain toughness;
step 2, wrapping the soft inner core obtained in the step 1 with an external wrapping layer added with a phagostimulant, and baking and forming again to obtain the oral immunization or administration 'spicy strips';
and 3, carrying out single plastic packaging on the oral immunization or administration spicy strips obtained in the step 2 by using food-grade film bags, and filling inert gas into the packaging to protect the oral immunization or administration spicy strips.
7. The production method according to claim 6,
step 1 and step 2, can also be toasted or steamed and toasted and finished in one step, namely finish the preparation before the inner core of step 1 is steamed and toasted, carry on step 2 subsequently, then toast or steam and toast and finish in one step; diluting the phagostimulant to a certain concentration and brushing the diluted phagostimulant on the surface of the wrapping layer.
8. The production method according to claim 6, wherein in the step 1,
the inner core is made from raw materials of flour, sugar, protein and baking soda;
the weight ratio (g: g) of the flour, the saccharides, the protein and the baking soda is (10-80): 5-20): 20-50): 1;
the volume-weight ratio (mL: g) of the added water to the total amount of the raw materials for making the inner core is 1: 1-1: 1.8.
9. The production method according to claim 7,
in the step 2, the weight ratio (g: g) of the external wrapping layer to the inner core is 1: 1-3: 1;
in the step 3, the inert gas is nitrogen or helium.
10. Use of the oral immunization or administration "peppers" according to any one of claims 1 to 5 for preparing oral immunization or administration for medium and large animals;
characterized in that the administration is of a common therapeutic agent; the oral immunization is vaccine oral immunization.
CN202210529516.XA 2022-05-16 2022-05-16 A Chinese medicinal composition for oral administration or administration of spicy food for middle-sized animals, and its preparation method Active CN115040468B (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1529615A (en) * 2000-06-30 2004-09-15 Method and composition for oral vaccination
CN103690964A (en) * 2013-12-20 2014-04-02 大连赛姆生物工程技术有限公司 Self-help-feeding drug carrier for sheep and preparation method thereof
CN106727426A (en) * 2016-12-08 2017-05-31 中国药科大学 A kind of preparation method for oral protein immune carrier

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1529615A (en) * 2000-06-30 2004-09-15 Method and composition for oral vaccination
CN103690964A (en) * 2013-12-20 2014-04-02 大连赛姆生物工程技术有限公司 Self-help-feeding drug carrier for sheep and preparation method thereof
CN106727426A (en) * 2016-12-08 2017-05-31 中国药科大学 A kind of preparation method for oral protein immune carrier

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