CN115029403A - Process and device for extracting polypeptide from momordica charantia - Google Patents
Process and device for extracting polypeptide from momordica charantia Download PDFInfo
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- CN115029403A CN115029403A CN202210915542.6A CN202210915542A CN115029403A CN 115029403 A CN115029403 A CN 115029403A CN 202210915542 A CN202210915542 A CN 202210915542A CN 115029403 A CN115029403 A CN 115029403A
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- B01F25/90—Falling particle mixers, e.g. with repeated agitation along a vertical axis with moving or vibrating means, e.g. stirrers, for enhancing the mixing
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- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
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Abstract
The invention discloses a wild bitter gourd polypeptide extraction process and a device, relating to the technical field of bitter gourd polypeptide extraction, wherein the device comprises an enzymolysis tank, a premixing mechanism and a dispersion mechanism, wherein the center position of the upper end of the enzymolysis tank is provided with an installation frame, the top of the installation frame is provided with a motor, the output end of the motor is vertically provided with a rotating shaft, the upper end of the enzymolysis tank is provided with a rotating groove, and the rotating groove is connected with a hopper in a sliding way. The overturning of the cleaning brush is realized, and the cleaning brush is convenient to wash.
Description
Technical Field
The invention relates to the technical field of bitter gourd polypeptide extraction, in particular to a technique and a device for extracting momordica charantia polypeptide.
Background
The balsam pear is a cucurbitaceae plant, and the whole plant comprises fruits with bitter taste, leaf edge teeth shape, rectangular fruits, emerald green tender fruits and orange after ripening; growing in subtropical regions, it is widely cultivated worldwide because it can be used as both a medicine and a vegetable. The fruit is bitter in property and cold in taste, has the effects of clearing summer heat, clearing away heat, improving eyesight, detoxifying and the like, is used for treating trauma by local external application, and is used for expelling parasites, regulating menstruation, resisting viruses and treating measles and hepatitis by oral administration.
The conventional bitter gourd polypeptide extraction mode generally adopts an enzymolysis method, namely, bitter gourd powder is directly subjected to enzymolysis by adopting cellulase, and then the aim of extraction is fulfilled by settling, filtering and collecting filtrate. The enzymolysis tank is added to most direct balsam pear powder to current mode, and the balsam pear powder agglomerates in the enzymolysis liquid easily, and traditional stirring mode is lower to the dispersion efficiency of balsam pear powder, influences the efficiency of enzymolysis liquid enzymolysis balsam pear powder, and then influence the extraction of balsam pear polypeptide, and the not convenient to use user uses.
Aiming at the problems, an improved momordica charantia polypeptide extraction process and device are designed.
Disclosure of Invention
The invention aims to provide a momordica charantia polypeptide extraction process and a momordica charantia polypeptide extraction device, which are used for solving the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a fructus Momordicae Charantiae polypeptide extraction process comprises the following steps;
the method comprises the following steps: and (3) putting the artificially planted and wild fresh balsam pears into a freeze drying device for freeze drying, and then crushing into powder to obtain the freeze-dried balsam pear powder.
Step two: pouring the balsam pear powder in the step one into an enzymolysis device, degrading the balsam pear powder by using cellulase (the enzymolysis temperature is 40 degrees, the enzymolysis time is 45 minutes), and then pouring the enzymolysis liquid into a barrel.
Step three: and (4) putting the barrel of the enzymatic hydrolysate in the step two into a refrigeration house for freezing and precipitating, wherein the temperature of the refrigeration house is not more than 0 ℃, the freezing time is 5 days, and the impurities are precipitated.
Step four: filtering the enzymolysis solution precipitated in the third step for three times by using a filtering device, then performing enzyme inactivation and disinfection (the temperature is within 100 ℃), and obtaining the balsam pear polypeptide concentrated solution.
An enzymolysis device for extracting momordica charantia polypeptide comprises an enzymolysis tank, a premixing mechanism and a dispersion mechanism, a discharge pipe for discharging is arranged at the lower end of the side wall of the enzymolysis tank, enzymolysis liquid is filled at the bottom of the enzymolysis tank, the center of the upper end of the enzymolysis tank is provided with a mounting frame, the upper end of the side wall of the enzymolysis tank is provided with a bracket for supporting the mounting frame, one end of the bracket far away from the enzymolysis tank is arranged on the side wall of the mounting frame, the top of the mounting frame is provided with a motor, the output end of the motor is vertically provided with a rotating shaft, one end of the rotating shaft far away from the motor is rotatably connected to the central position of the bottom of the enzymolysis tank, the upper end of enzymolysis tank has been seted up and has been rotated the groove, it has the hopper that is used for adding the balsam pear powder to enzymolysis tank inside to rotate sliding connection on the groove, installs the second puddler that is used for carrying out the stirring to the enzymolysis liquid on the rotation axis lateral wall that is close to the enzymolysis tank bottom.
The premixing mechanism is arranged inside the enzymolysis tank above the second stirring rod and is used for premixing bitter gourd powder and enzymolysis liquid, so that the bitter gourd powder is mixed with the enzymolysis liquid with low concentration, the mixed liquid is conveyed to the bottom of the enzymolysis tank, and the enzymolysis efficiency of the enzymolysis liquid on the bitter gourd powder is improved.
Dispersion mechanism sets up at the top of enzymolysis tank for disperse the bitter gourd powder, make the even whereabouts of bitter gourd powder, improve the mixing efficiency of bitter gourd powder and enzymolysis liquid.
As a still further scheme of the invention: premix the mechanism and include the baffle, baffle horizontal installation is on the enzymolysis tank inner wall of second puddler top, the central point of baffle puts to rotate the cover on the lateral wall of rotation axis, install the drinking-water pipe on the baffle, the top layer of enzymolysis liquid is inserted to the lower extreme of drinking-water pipe, install on the lateral wall of drinking-water pipe and be used for making the enzymolysis liquid carry the second water pump of baffle upper end through the drinking-water pipe, the lower extreme at the baffle is installed to the second water pump, install the first puddler that is used for mixing balsam pear powder and enzymolysis liquid on the rotation axis lateral wall of baffle top, install the catheter on the enzymolysis tank lateral wall of baffle top, the one end that first puddler was kept away from to the catheter is installed at the lateral wall lower extreme of enzymolysis tank, install on the lateral wall of catheter and be used for carrying the mixed liquid of baffle top through the first water pump of catheter enzymolysis tank bottoms, the first water pump is installed on the lateral wall of the enzymolysis tank.
As a still further scheme of the invention: the dispersion mechanism comprises a rotary drum, the rotary drum is rotatably sleeved on the side wall of the rotary shaft above the first stirring rod, the upper end of the rotary drum is rotatably connected to the top of the enzymolysis tank, a second gear is installed on the side wall of the rotary shaft inside the rotary drum, a first gear is meshed with the side wall of the second gear, the first gear is rotatably connected to the top of the enzymolysis tank inside the rotary drum through a support rod, a second transmission toothed ring matched with the first gear is installed on the inner wall of the rotary drum, the second transmission toothed ring is meshed with one end of the first gear away from the second gear, a dispersion leakage hole plate used for dispersing bitter gourd powder is rotatably sleeved on the side wall of the rotary drum, the side wall of the dispersion leakage hole plate is installed on the inner wall of the enzymolysis tank above the input end of a liquid guide pipe, and a bitter gourd powder stirring plate used for dispersing the upper end of the dispersion leakage hole plate is installed on the side wall of the rotary drum above the dispersion leakage hole plate, the lower extreme of dialling the board hugs closely in the upper end of dispersion orifice plate, the inside material subassembly that spills that is used for making the hopper to evenly spilling the material of dispersion orifice plate that is provided with of enzymolysis tank above the dispersion orifice plate, the lower extreme of dispersion orifice plate is provided with and is used for clearing up the clearance subassembly of preventing stifled to dispersion orifice plate lower extreme.
As a still further scheme of the invention: the material spreading assembly comprises a fixed frame which is arranged on the side wall of the rotary cylinder above the dispersing perforated plate, the bottom of the fixed frame is horizontally provided with a reciprocating screw rod, two ends of the reciprocating screw rod are respectively and rotatably connected on the side wall of the fixed frame, the side wall of the reciprocating screw is rotationally connected with a moving block through threads, the side wall of the moving block is closely attached to and slides on the inner wall of the fixed frame, the output end of the hopper penetrates through the top of the fixed frame and is provided with a hose, one end of the hose far away from the hopper is provided with a discharge pipe, the discharge pipe is arranged on the side wall of the moving block, a limiting frame is arranged on the inner wall of the enzymolysis tank close to the reciprocating screw rod, first transmission ring gear is installed to the bottom of spacing frame, the one end that the rotation section of thick bamboo was kept away from to reciprocal screw rod is passed fixed frame and spacing frame and is installed the third gear that uses with the cooperation of first transmission ring gear, third gear and first transmission ring gear intermeshing.
As a still further scheme of the invention: the cleaning component comprises a rotating rod and a limiting groove, the limiting groove is horizontally arranged on the inner wall of the enzymolysis tank below the input end of the liquid guide pipe, the rotating rod is horizontally arranged in the enzymolysis tank below the input end of the liquid guide pipe, one end of the rotating rod, which is close to the rotating cylinder, is rotatably connected to the side wall of the rotating cylinder, a plurality of connecting rods which are oppositely arranged are arranged on the side wall of the rotating rod, one end, which is far away from the rotating rod, of each connecting rod is provided with a cleaning brush for cleaning the lower end of the scattered perforated plate, the cleaning brushes and the connecting rods are on the same plane, one end, which is far away from the rotating cylinder, of the rotating rod is provided with a limiting plate for limiting the rotating rod, the limiting plate is slidably connected to the inner wall of the limiting groove, an opening is formed in the limiting groove, a rotating frame convenient for rotating the limiting plate to rotate is arranged in the opening, and guide plates, convenient for the limiting plate to enter the limiting groove are arranged at the two ends of the rotating frame, the deflector is kept away from the one end of rotating the frame and is installed at the both ends of spacing groove, the inside of rotating the section of thick bamboo is provided with and is used for utilizing reciprocal screw rod pivoted power to drive the linkage subassembly of dwang and clearance brush upset.
As a still further scheme of the invention: the linkage assembly comprises a connecting cylinder, the connecting cylinder is sleeved on the side wall of a rotating shaft in the rotating cylinder, a second bevel gear is installed at the upper end of the connecting cylinder, an incomplete bevel gear is installed at the lower end of the connecting cylinder, the lower ends of the second bevel gear and the incomplete bevel gear are both rotatably connected with a rotating seat, the rotating seat is installed on the inner wall of the rotating cylinder, a third bevel gear is installed at one end, close to the rotating cylinder, of the reciprocating screw rod, penetrates through the side walls of the fixed frame and the rotating cylinder, the third bevel gear is meshed with the second bevel gear, the diameter of the second bevel gear is larger than that of the third bevel gear, a first bevel gear is installed at one end, close to the rotating cylinder, of the rotating rod, penetrates through the side wall of the rotating cylinder, the first bevel gear is meshed with the incomplete bevel gear, the diameter of the incomplete bevel gear is larger than that of the first bevel gear, and when the limiting plate moves into the rotating cylinder, incomplete bevel gear and first bevel gear intermeshing, incomplete tooth on the incomplete bevel gear can drive first bevel gear and rotate half a circle.
As a still further scheme of the invention: and a reinforcing rib plate for improving the structural strength of the bracket is arranged on the side wall of the bracket.
As a still further scheme of the invention: install on the inner wall of enzymolysis tank and drive the connecting rod when the connecting rod upset and carry out the vibrator that vibrates.
As a still further scheme of the invention: the side wall of the liquid guide pipe is provided with a pipe hoop used for fixing the liquid guide pipe, and the pipe hoop is arranged on the side wall of the enzymolysis tank.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention is provided with the premixing mechanism, and because the enzymolysis time is longer, the active ingredients of the enzymolysis liquid easily sink, so that the top layer liquid of the enzymolysis liquid is conveyed to the partition plate by using the water pumping pipe, the enzymolysis liquid and the bitter gourd powder are premixed by using the first stirring rod and subjected to preliminary enzymolysis, then the mixed liquid is conveyed to the bottom of the enzymolysis tank through the liquid guide pipe, the bitter gourd powder is subjected to enzymolysis again by using the high-concentration enzymolysis liquid, and simultaneously, the bitter gourd powder dissolved in the enzymolysis liquid is more easily diffused and dispersed at the bottom of the enzymolysis tank, and the enzymolysis efficiency of the enzymolysis liquid on the bitter gourd powder is effectively improved.
2. The bitter gourd powder dispersing device is provided with the dispersing mechanism, the rotating cylinder is decelerated by the second gear, the first gear and the second transmission gear ring, the rotating cylinder drives the shifting plate to move slowly, the shifting plate effectively disperses bitter gourd powder at the upper end of the dispersing leakage orifice plate, the bitter gourd powder at the upper end of the dispersing leakage orifice plate uniformly falls onto the partition plate, and the mixing efficiency of the bitter gourd powder and enzymatic hydrolysate is improved.
3. The bitter gourd powder dispersing device is provided with the material scattering assembly, the rotating cylinder is used for driving the fixing frame and the hopper to rotate so as to drive the discharging pipe to rotate, meanwhile, the reciprocating screw rod is used for driving the discharging pipe to reciprocate, so that the discharging pipe can uniformly scatter materials to the dispersing leakage orifice plate, the shifting plate can rapidly disperse bitter gourd powder on the dispersing leakage orifice plate, the dispersing efficiency of the bitter gourd powder at the upper end of the dispersing leakage orifice plate is improved, and meanwhile, the mixing efficiency of the bitter gourd powder and an enzymolysis liquid is improved.
4. The cleaning brush is provided with the cleaning assembly, the rotating cylinder is used for driving the rotating rod to rotate, so that the rotating rod drives the cleaning brush to clean the lower end of the dispersing perforated plate, the lower end of the dispersing perforated plate is prevented from being blocked, the dispersing efficiency of the balsam pear powder is improved, and meanwhile, the limiting groove and the rotating rod are arranged below the input end of the liquid guide pipe, so that the cleaning brush can be rinsed through overturning the cleaning brush, and the cleaning efficiency of the cleaning brush on the lower end of the dispersing perforated plate is improved.
5. The invention is provided with a linkage component, a third bevel gear is driven to rotate by the rotation of a reciprocating screw rod, the third bevel gear drives a second bevel gear to slowly rotate, the second bevel gear drives an incomplete bevel gear to rotate through a connecting cylinder, when incomplete teeth on the incomplete bevel gear are meshed with a first bevel gear, the incomplete bevel gear drives the first bevel gear to rotate for half a circle, so that the first bevel gear drives a rotating rod to rotate for half a circle, the overturning of a cleaning brush is realized, the cleaning brush is convenient to rinse, and the cleaning efficiency of the cleaning brush on the lower end of a dispersion hole plate is improved.
Drawings
FIG. 1 is a schematic three-dimensional structure of the present invention.
Fig. 2 is a schematic top view of the present invention.
Fig. 3 is a schematic bottom view of the present invention.
FIG. 4 is a top view of the linkage assembly of the present invention.
FIG. 5 is a bottom view of the linkage assembly of the present invention.
Wherein: 1. an enzymolysis tank; 2. a rotating shaft; 3. a partition plate; 4. a limiting groove; 5. a rotating drum; 6. a dispersive perforated plate; 7. dialing a plate; 8. a hose; 9. a reciprocating screw; 10. a support; 11. a hopper; 12. installing a frame; 13. a motor; 14. a rotating groove; 15. a fixing frame; 16. a limiting frame; 17. a first drive ring gear; 18. a catheter; 19. rotating the frame; 20. rotating the rod; 21. a connecting rod; 22. cleaning a brush; 23. a first stirring rod; 24. a second stirring rod; 25. a first water pump; 26. a water pumping pipe; 27. a second water pump; 28. a first bevel gear; 29. a rotating seat; 30. a second bevel gear; 31. a third bevel gear; 32. a moving block; 33. a discharge pipe; 34. a connecting cylinder; 35. an incomplete bevel gear; 36. a limiting plate; 37. a guide plate; 38. a second drive ring gear; 39. a first gear; 40. a second gear; 41. a third gear.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the invention, the momordica charantia polypeptide extraction process comprises the following steps;
the method comprises the following steps: and (3) putting the artificially planted and wild fresh balsam pears into a freeze drying device for freeze drying, and then crushing into powder to obtain the freeze-dried balsam pear powder.
Step two: pouring the balsam pear powder in the step one into an enzymolysis device, degrading the balsam pear powder by using cellulase (the enzymolysis temperature is 40 degrees, the enzymolysis time is 45 minutes), and then pouring the enzymolysis liquid into a barrel.
Step three: and (4) putting the barrel containing the enzymatic hydrolysate in the step two into a refrigeration house for freezing and precipitating, wherein the temperature of the refrigeration house is not more than 0 ℃, the freezing time is 5 days, and the impurities are precipitated.
Step four: filtering the enzymolysis solution precipitated in the third step for three times by using a filtering device, then performing enzyme inactivation, and sterilizing (the temperature is within 100 ℃), thus obtaining the balsam pear polypeptide concentrated solution.
Referring to fig. 1-5, an enzymolysis device for extracting momordica charantia polypeptide comprises an enzymolysis tank 1, a premixing mechanism and a dispersing mechanism, wherein a discharge pipe for discharging is installed at the lower end of the side wall of the enzymolysis tank 1, enzymolysis liquid is filled in the bottom of the enzymolysis tank 1, an installation frame 12 is installed at the central position of the upper end of the enzymolysis tank 1, a support 10 for supporting the installation frame 12 is installed at the upper end of the side wall of the enzymolysis tank 1, one end, far away from the enzymolysis tank 1, of the support 10 is installed on the side wall of the installation frame 12, a motor 13 is installed at the top of the installation frame 12, a rotating shaft 2 is vertically installed at the output end of the motor 13, one end, far away from the motor 13, of the rotating shaft 2 is rotatably connected to the central position of the bottom of the enzymolysis tank 1, a rotating groove 14 is formed in the upper end of the enzymolysis tank 1, a hopper 11 for adding momordica charantia powder into the enzymolysis tank 1 is slidably connected to the rotating groove 14, a second stirring rod 24 for stirring the enzymolysis liquid is arranged on the side wall of the rotating shaft 2 close to the bottom of the enzymolysis tank 1.
Premixing mechanism sets up inside enzymolysis tank 1 above second puddler 24 for premix bitter gourd powder and enzymolysis liquid, make bitter gourd powder mix with the enzymolysis liquid of low concentration earlier, then carry the bottom of enzymolysis tank 1 with the mixed liquid after will mixing, improve the enzymolysis efficiency of enzymolysis liquid to bitter gourd powder.
Dispersing mechanism sets up at the top of enzymolysis tank 1 for disperse the bitter gourd powder, make the even whereabouts of bitter gourd powder, improve the mixing efficiency of bitter gourd powder and enzymolysis liquid.
Premix mechanism includes baffle 3, 3 horizontal installation of baffle is on the 1 inner wall of enzymolysis tank in second puddler 24 top, the central point of baffle 3 puts and rotates the cover on the lateral wall of rotation axis 2, install drinking-water pipe 26 on the baffle 3, the top layer of enzymolysis liquid is inserted to the lower extreme of drinking-water pipe 26, install on the lateral wall of drinking-water pipe 26 and be used for making the enzymolysis liquid carry the second water pump 27 of baffle 3 upper end through drinking-water pipe 26, the lower extreme at baffle 3 is installed to second water pump 27, install on the 2 lateral walls of rotation axis of baffle 3 top and be used for carrying out the first puddler 23 that mixes to balsam pear powder and enzymolysis liquid, install catheter 18 on the enzymolysis tank 1 lateral wall of baffle 3 top, catheter 18 is kept away from the one end of first puddler 23 and is installed at the lateral wall lower extreme of enzymolysis tank 1, install on catheter 18's the lateral wall and be used for carrying the mixed liquid of baffle 3 top through catheter 18 the first water of enzymolysis tank 1 bottom And the pump 25, wherein the first water pump 25 is arranged on the side wall of the enzymolysis tank 1.
The effect of mechanism in advance is because the enzymolysis time is longer, the active ingredient of enzymolysis liquid sinks easily, thereby utilize drinking-water pipe 26 to carry the top layer liquid of enzymolysis liquid on the baffle 3, and utilize first puddler 23 to premix enzymolysis liquid and balsam pear powder, and carry out preliminary enzymolysis, then carry the bottom of enzymolysis tank 1 through catheter 18 with mixed liquid, the enzymolysis liquid that utilizes high concentration carries out the enzymolysis to the balsam pear powder once more, the balsam pear powder that has dissolved in the enzymolysis liquid simultaneously is dispersed in the diffusion in enzymolysis tank 1 bottom more easily, effectively improve the enzymolysis efficiency of enzymolysis liquid to the balsam pear powder.
The dispersion mechanism comprises a rotary drum 5, the rotary drum 5 is rotatably sleeved on the side wall of the rotary shaft 2 above the first stirring rod 23, the upper end of the rotary drum 5 is rotatably connected to the top of the enzymolysis tank 1, the side wall of the rotary shaft 2 inside the rotary drum 5 is provided with a second gear 40, the side wall of the second gear 40 is meshed with a first gear 39, the first gear 39 is rotatably connected to the top of the enzymolysis tank 1 inside the rotary drum 5 through a support rod, the inner wall of the rotary drum 5 is provided with a second transmission toothed ring 38 matched with the first gear 39 for use, the second transmission toothed ring 38 is meshed with one end of the first gear 39 far away from the second gear 40, the side wall of the rotary drum 5 is rotatably sleeved with a dispersion leakage hole plate 6 used for dispersing bitter gourd powder, the side wall of the dispersion leakage hole plate 6 is installed on the inner wall of the enzymolysis tank 1 above the input end of the liquid guide tube 18, install on the 5 lateral walls of a rotation section of thick bamboo above the dispersion orifice plate 6 and be used for carrying out the bitter gourd powder that disperses to the 6 upper ends of dispersion orifice plate and dial board 7, the lower extreme of dialling board 7 is hugged closely in the upper end of dispersion orifice plate 6, 1 inside of enzymolysis tank above the dispersion orifice plate 6 is provided with and is used for making hopper 11 to the scattered material subassembly that spills that leaks 6 even spills of orifice plate, the lower extreme of dispersion orifice plate 6 is provided with and is used for clearing up the clearance subassembly that prevents stifled to 6 lower extremes of dispersion orifice plate.
The effect of dispersion mechanism utilizes second gear 40, first gear 39 and second transmission ring gear 38 to slow down to a rotating cylinder 5, makes a rotating cylinder 5 drive and dials board 7 slow moving, realizes dialling the effective dispersion of board 7 to dispersion leak orifice plate 6 upper end balsam pear powder, makes the even dropping of balsam pear powder on the 6 upper ends of dispersion leak orifice plate baffle 3, improves the mixing efficiency of balsam pear powder and enzymolysis liquid, and convenient to use person uses.
The material scattering assembly comprises a fixed frame 15, the fixed frame 15 is installed on the side wall of the rotating cylinder 5 above the dispersing perforated plate 6, a reciprocating screw 9 is horizontally arranged at the bottom of the fixed frame 15, the two ends of the reciprocating screw 9 are respectively connected onto the side wall of the fixed frame 15 in a rotating manner, the side wall of the reciprocating screw 9 is connected with a moving block 32 in a rotating manner through threads, the side wall of the moving block 32 is tightly attached to the inner wall of the fixed frame 15 in a sliding manner, the output end of the hopper 11 penetrates through the top of the fixed frame 15 and is provided with a hose 8, one end, far away from the hopper 11, of the hose 8 is provided with a discharging pipe 33, the discharging pipe 33 is installed on the side wall of the moving block 32, a limiting frame 16 is installed on the inner wall of the enzymolysis tank 1 close to the reciprocating screw 9, a first transmission toothed ring 17 is installed at the bottom of the limiting frame 16, one end, far away from the rotating cylinder 5, of the reciprocating screw 9 penetrates through the fixed frame 15 and the limiting frame 16 and is provided with a third gear 41 matched with the first transmission toothed ring 17, the third gear wheel 41 is intermeshed with the first transmission ring gear 17.
The effect of spilling the material subassembly is that it drives fixed frame 15 and hopper 11 rotation to utilize a rotation section of thick bamboo 5 to drive discharging pipe 33 and rotate, utilize reciprocal screw rod 9 to drive discharging pipe 33 reciprocating motion simultaneously, realize discharging pipe 33 and to the even material of spilling of scattered orifice plate 6, be convenient for dial board 7 and to disperse the bitter gourd powder on the orifice plate 6 and carry out the fast dispersion, improve the dispersion efficiency of 6 upper end bitter gourd powder of scattered orifice plate, improve the mixing efficiency of bitter gourd powder and enzymolysis liquid simultaneously, convenient to use person uses.
The cleaning component comprises a rotating rod 20 and a limiting groove 4, the limiting groove 4 is horizontally arranged on the inner wall of an enzymolysis tank 1 below the input end of a liquid guide tube 18, the rotating rod 20 is horizontally arranged inside the enzymolysis tank 1 below the input end of the liquid guide tube 18, one end of the rotating rod 20, which is close to the rotating cylinder 5, is rotatably connected onto the side wall of the rotating cylinder 5, a plurality of connecting rods 21 which are oppositely arranged are arranged on the side wall of the rotating rod 20, a cleaning brush 22 for cleaning the lower end of a dispersing leak orifice plate 6 is arranged at one end, which is far away from the rotating rod 20, of each connecting rod 21, the cleaning brush 22 and the connecting rod 21 are arranged on the same plane, a limiting plate 36 for limiting the rotating rod 20 is arranged at one end, which is far away from the rotating cylinder 5, of the rotating rod 20, the limiting plate 36 is slidably connected onto the inner wall of the limiting groove 4, an opening is arranged on the limiting groove 4, and a rotating frame 19 convenient for limiting plate 36 to rotate is arranged in the opening, rotate the both ends of frame 19 and install the deflector 37 that is convenient for limiting plate 36 to enter into the spacing groove 4, the deflector 37 is kept away from the one end of rotating frame 19 and is installed at the both ends of spacing groove 4, the inside of rotating cylinder 5 is provided with and is used for utilizing reciprocal screw rod 9 pivoted power to drive the linkage subassembly of dwang 20 and the upset of cleaning brush 22.
The effect of clearance subassembly utilizes a rotor section of thick bamboo 5 to drive dwang 20 and rotates to make dwang 20 drive clearance brush 22 clear up the lower extreme that disperses hourglass orifice plate 6, prevent that 6 lower extremes of dispersion hourglass orifice plate from blockking up, improve the dispersion efficiency of balsam pear powder, simultaneously because spacing groove 4 and dwang 20 set up the below at catheter 18 input, can rinse clearance brush 22 through the upset to clearance brush 22, improve clearance brush 22 and to the clearance efficiency of dispersing hourglass orifice plate 6 lower extreme.
The linkage assembly comprises a connecting cylinder 34, the connecting cylinder 34 is sleeved on the side wall of the rotating shaft 2 in the rotating cylinder 5, a second bevel gear 30 is installed at the upper end of the connecting cylinder 34, an incomplete bevel gear 35 is installed at the lower end of the connecting cylinder 34, the lower ends of the second bevel gear 30 and the incomplete bevel gear 35 are both rotatably connected with a rotating seat 29, the rotating seat 29 is installed on the inner wall of the rotating cylinder 5, one end of the reciprocating screw 9 close to the rotating cylinder 5 penetrates through the fixed frame 15 and the side wall of the rotating cylinder 5 to be provided with a third bevel gear 31, the third bevel gear 31 is mutually meshed with the second bevel gear 30, the diameter of the second bevel gear 30 is larger than that of the third bevel gear 31, one end of the rotating rod 20 close to the rotating cylinder 5 penetrates through the side wall of the rotating cylinder 5 to be provided with a first bevel gear 28, and the first bevel gear 28 is mutually meshed with the incomplete bevel gear 35, the diameter of the incomplete bevel gear 35 is larger than that of the first bevel gear 28, when the limiting plate 36 moves to the inside of the rotating frame 19, the incomplete bevel gear 35 is meshed with the first bevel gear 28, and incomplete teeth on the incomplete bevel gear 35 can drive the first bevel gear 28 to rotate for half a turn.
The effect of linkage subassembly utilizes the rotation of reciprocal screw rod 9 to drive third bevel gear 31 and rotates, third bevel gear 31 drives second bevel gear 30 and slowly rotates, second bevel gear 30 passes through connecting cylinder 34 and drives incomplete bevel gear 35 and rotate, when incomplete tooth on incomplete bevel gear 35 and first bevel gear 28 meshing, incomplete bevel gear 35 drives first bevel gear 28 and rotates the half-turn, thereby make first bevel gear 28 drive dwang 20 and rotate the half-turn, realize the upset of clearance brush 22, be convenient for the rinse-wash of clearance brush 22, improve clearance brush 22 and to the clearance efficiency of scattered orifice plate 6 lower extreme, convenient to use person uses.
When using, start second water pump 27 earlier, second water pump 27 carries the upper end of baffle 3 with the enzymolysis liquid supernatant through drinking-water pipe 26, inside the staff added the hopper 11 with the bitter gourd powder, the inside of discharging pipe 33 is carried through hose 8 with the bitter gourd powder to hopper 11, start motor 13 simultaneously, the output of motor 13 drives rotation axis 2 and rotates, rotation axis 2 drives first puddler 23 and second puddler 24 and stirs the enzymolysis liquid, rotation axis 2 drives second gear 40 simultaneously and rotates, second gear 40 drives first gear 39 and rotates, first gear 39 drives second transmission ring gear 38 and rotates, second transmission ring gear 38 drives a rotating cylinder 5 and rotates.
The rotating cylinder 5 drives the fixed frame 15 and the hopper 11 to rotate, the fixed frame 15 drives the reciprocating screw 9 to rotate, the reciprocating screw 9 drives the third gear 41 to roll along the first transmission gear ring 17, the third gear 41 drives the reciprocating screw 9 to rotate, under the action of the screw thread, the reciprocating screw 9 drives the moving block 32 to reciprocate, the moving block 32 drives the discharging pipe 33 to reciprocate, so that the discharge pipe 33 uniformly sprays the bitter gourd powder on the upper end of the dispersing perforated plate 6, the rotary drum 5 drives the stirring plate 7 to rotate, the stirring plate 7 disperses the bitter gourd powder on the dispersing perforated plate 6, the bitter gourd powder on the dispersing perforated plate 6 falls onto the partition plate 3 and is mixed under the action of the first stirring rod 23, then, the first water pump 25 is started, and the mixed solution is delivered to the bottom of the enzymolysis tank 1 through the liquid guide tube 18 by the first water pump 25, and is fully mixed with the high-concentration enzymolysis solution for enzymolysis.
Meanwhile, the rotating cylinder 5 drives the rotating rod 20 to rotate, the rotating rod 20 drives the connecting rod 21 to rotate, the connecting rod 21 drives the cleaning brush 22 to clean the lower end of the dispersing leak hole plate 6, meanwhile, the reciprocating screw 9 drives the third bevel gear 31 to rotate, the third bevel gear 31 drives the second bevel gear 30 to rotate, the second bevel gear 30 drives the connecting cylinder 34 to rotate, the connecting cylinder 34 drives the incomplete bevel gear 35 to rotate, when the limiting plate 36 moves into the rotating frame 19, the incomplete bevel gear 35 is meshed with the first bevel gear 28, the incomplete bevel gear 35 drives the first bevel gear 28 to rotate for a half-turn, the first bevel gear 28 drives the rotating rod 20 to rotate, the rotating rod 20 drives the connecting rod 21 and the cleaning brush 22 to rotate, the cleaning brush 22 is turned over, the rotating rod 20 drives the limiting plate 36 to turn over in the rotating frame 19, after the turning over is completed, as the rotating lever 20 rotates, the stopper plate 36 reenters the inside of the stopper groove 4 to restrict the rotating lever 20.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof.
Claims (10)
1. A momordica charantia polypeptide extraction process is characterized by comprising the following steps;
the method comprises the following steps: freeze-drying the artificially-planted and wild fresh bitter gourds in a freeze-drying device, and then crushing into powder to obtain freeze-dried bitter gourd powder;
step two: pouring the balsam pear powder in the step one into an enzymolysis device, degrading the balsam pear powder by using cellulase (the enzymolysis temperature is 40 degrees, the enzymolysis time is 45 minutes), and then pouring the enzymolysis liquid into a barrel;
step three: putting the barrel containing the enzymatic hydrolysate in the step two into a refrigeration house for freezing and precipitating, wherein the temperature of the refrigeration house is not more than 0 ℃, the freezing time is 5 days, and impurities are precipitated;
step four: filtering the enzymolysis solution precipitated in the third step for three times by using a filtering device, then performing enzyme inactivation and disinfection (the temperature is within 100 ℃), and obtaining the balsam pear polypeptide concentrated solution.
2. The enzymatic hydrolysis device for momordica charantia polypeptide extraction according to claim 1, comprising an enzymatic hydrolysis tank (1), a premixing mechanism and a dispersing mechanism, wherein a discharging pipe for discharging is installed at the lower end of the side wall of the enzymatic hydrolysis tank (1), enzymatic hydrolysis liquid is filled in the bottom of the enzymatic hydrolysis tank (1), a mounting frame (12) is installed at the central position of the upper end of the enzymatic hydrolysis tank (1), a support (10) for supporting the mounting frame (12) is installed at the upper end of the side wall of the enzymatic hydrolysis tank (1), one end of the support (10) far away from the enzymatic hydrolysis tank (1) is installed on the side wall of the mounting frame (12), a motor (13) is installed at the top of the mounting frame (12), a rotating shaft (2) is vertically installed at the output end of the motor (13), one end of the rotating shaft (2) far away from the motor (13) is rotatably connected to the central position of the bottom of the enzymatic hydrolysis tank (1), a rotating groove (14) is formed in the upper end of the enzymolysis tank (1), a hopper (11) used for adding bitter gourd powder into the enzymolysis tank (1) is connected onto the rotating groove (14) in a sliding mode, and a second stirring rod (24) used for stirring enzymolysis liquid is installed on the side wall of the rotating shaft (2) close to the bottom of the enzymolysis tank (1);
the premixing mechanism is arranged in the enzymolysis tank (1) above the second stirring rod (24) and is used for premixing the bitter gourd powder and the enzymolysis liquid and then conveying the mixed liquid to the bottom of the enzymolysis tank (1);
the dispersion mechanism is arranged at the top of the enzymolysis tank (1) and used for dispersing the bitter gourd powder to enable the bitter gourd powder to fall evenly.
3. The enzymatic hydrolysis device for mountain balsam pear polypeptide extraction of claim 2, characterized in that, it includes baffle (3) to mix the mechanism in advance, baffle (3) horizontal installation is on enzymolysis tank (1) inner wall of second puddler (24) top, the central point of baffle (3) puts the rotation cover on the lateral wall of rotation axis (2), install drinking-water pipe (26) on baffle (3), the top layer of enzymolysis liquid is inserted to the lower extreme of drinking-water pipe (26), install on the lateral wall of drinking-water pipe (26) and be used for making the enzymolysis liquid carry second water pump (27) of baffle (3) upper end through drinking-water pipe (26), the lower extreme at baffle (3) is installed in second water pump (27), install on the rotation axis (2) lateral wall of baffle (3) top and be used for carrying out the first puddler (23) that mix balsam pear powder and enzymolysis liquid, install catheter (18) on enzymolysis tank (1) lateral wall of baffle (3) top, the lateral wall lower extreme at enzymolysis tank (1) is installed to the one end that first puddler (23) were kept away from in catheter (18), install on the lateral wall of catheter (18) and be used for carrying first water pump (25) of enzymolysis tank (1) bottom with the mixed liquid of baffle (3) top through catheter (18), install on the lateral wall of enzymolysis tank (1) first water pump (25).
4. The enzymatic hydrolysis device for momordica dioica polypeptide extraction according to claim 2, wherein the dispersion mechanism comprises a rotating cylinder (5), the rotating cylinder (5) is rotatably sleeved on the side wall of the rotating shaft (2) above the first stirring rod (23), the upper end of the rotating cylinder (5) is rotatably connected to the top of the enzymatic hydrolysis tank (1), the side wall of the rotating shaft (2) inside the rotating cylinder (5) is provided with a second gear (40), the side wall of the second gear (40) is engaged with a first gear (39), the first gear (39) is rotatably connected to the top of the enzymatic hydrolysis tank (1) inside the rotating cylinder (5) through a support rod, the inner wall of the rotating cylinder (5) is provided with a second transmission gear ring (38) matched with the first gear (39) for use, and the second transmission gear ring (38) and the first gear (39) are mutually engaged with one end of the second gear (40) far away from the first gear (39), rotate the cover on the lateral wall of a section of thick bamboo (5) and be used for carrying out dispersion hourglass orifice plate (6) that disperse to the balsam pear powder, the lateral wall of dispersion hourglass orifice plate (6) is installed on enzymolysis tank (1) inner wall above catheter (18) input, install on rotation section of thick bamboo (5) lateral wall of dispersion hourglass orifice plate (6) top and be used for carrying out dispersion group board (7) to the balsam pear powder of dispersion hourglass orifice plate (6) upper end, the lower extreme of group board (7) is hugged closely in the upper end of dispersion hourglass orifice plate (6), enzymolysis tank (1) inside of dispersion hourglass orifice plate (6) top is provided with and is used for making hopper (11) to the scattered material subassembly that spills material of dispersion hourglass orifice plate (6), the lower extreme of dispersion hourglass orifice plate (6) is provided with and is used for clearing up the clearance subassembly that prevents blockking up to dispersion hourglass orifice plate (6) lower extreme.
5. The enzymolysis device for extracting momordica dioica polypeptide according to claim 4, wherein said spreading component comprises a fixed frame (15), said fixed frame (15) is installed on the side wall of the rotating cylinder (5) above the dispersing perforated plate (6), the bottom of said fixed frame (15) is horizontally provided with a reciprocating screw (9), two ends of said reciprocating screw (9) are respectively connected on the side wall of the fixed frame (15) in a rotating way, the side wall of said reciprocating screw (9) is connected with a moving block (32) in a rotating way by screw threads, the side wall of said moving block (32) is tightly attached to the inner wall of the fixed frame (15) in a sliding way, the output end of said hopper (11) passes through the top of the fixed frame (15) and is installed with a hose (8), the end of said hose (8) far away from the hopper (11) is installed with a discharging pipe (33), said discharging pipe (33) is installed on the side wall of the moving block (32), install limit frame (16) on enzymolysis tank (1) inner wall near reciprocating screw rod (9), first transmission ring gear (17) are installed to the bottom of limit frame (16), reciprocating screw rod (9) are kept away from the one end of rotating a section of thick bamboo (5) and are passed fixed frame (15) and limit frame (16) and install third gear (41) with first transmission ring gear (17) cooperation use, third gear (41) and first transmission ring gear (17) intermeshing.
6. The enzymolysis device for mountain balsam pear polypeptide extraction of claim 4, characterized in that, the clearance subassembly includes dwang (20) and spacing groove (4), spacing groove (4) horizontal installation is on enzymolysis tank (1) inner wall below catheter (18) input, dwang (20) level sets up inside enzymolysis tank (1) below catheter (18) input, the one end that dwang (20) is close to a rotating barrel (5) is rotated and is connected on the lateral wall of a rotating barrel (5), install connecting rod (21) that a plurality of set up relatively on the lateral wall of dwang (20), the one end that dwang (20) was kept away from in connecting rod (21) is installed and is used for carrying out the clearance to the lower extreme that disperses leak orifice plate (6) (22), clearance brush (22) and connecting rod (21) are on the coplanar, dwang (20) are kept away from the one end of a rotation section of thick bamboo (5) and are installed and be used for carrying out spacing limiting plate (36) to dwang (20), limiting plate (36) sliding connection is on the inner wall of spacing groove (4), be provided with the opening on spacing groove (4), be provided with limiting plate (36) pivoted rotation frame (19) of being convenient for in the opening, install at the both ends of rotation frame (19) and be convenient for limiting plate (36) to enter into deflector (37) in spacing groove (4), deflector (37) are kept away from the one end of rotation frame (19) and are installed at the both ends of spacing groove (4), the inside of a rotation section of thick bamboo (5) is provided with and is used for utilizing reciprocal screw rod (9) pivoted power to drive the linkage subassembly of dwang (20) and clearance brush (22) upset.
7. The enzymatic hydrolysis device for momordica dioica polypeptide extraction according to claim 6, wherein the linkage assembly comprises a connecting cylinder (34), the connecting cylinder (34) is sleeved on the side wall of the rotating shaft (2) inside the rotating cylinder (5), the upper end of the connecting cylinder (34) is provided with a second bevel gear (30), the lower end of the connecting cylinder (34) is provided with an incomplete bevel gear (35), the lower ends of the second bevel gear (30) and the incomplete bevel gear (35) are both rotatably connected with a rotating seat (29), the rotating seat (29) is arranged on the inner wall of the rotating cylinder (5), one end of the reciprocating screw (9) close to the rotating cylinder (5) penetrates through the side walls of the fixed frame (15) and the rotating cylinder (5) to be provided with a third bevel gear (31), the third bevel gear (31) is meshed with the second bevel gear (30), and the diameter of the second bevel gear (30) is larger than that of the third bevel gear (31), the utility model discloses a rotating frame, including dwang (20), first bevel gear (28) are installed to the lateral wall that the one end of dwang (20) is close to a rotation section of thick bamboo (5) passes a rotation section of thick bamboo (5), first bevel gear (28) and incomplete bevel gear (35) intermeshing, the diameter of incomplete bevel gear (35) is greater than the diameter of first bevel gear (28), and when limiting plate (36) moved inside rotation frame (19), incomplete bevel gear (35) and first bevel gear (28) intermeshing, incomplete tooth on incomplete bevel gear (35) can drive first bevel gear (28) and rotate the half-turn.
8. The enzymatic hydrolysis device for momordica dioica polypeptide extraction according to claim 2, wherein the side wall of the bracket (10) is installed with reinforcing ribs for improving the structural strength of the bracket (10).
9. The enzymolysis device for mountain balsam pear polypeptide extraction according to claim 6, wherein a vibrator for driving the connecting rod (21) to vibrate when the connecting rod (21) is overturned is installed on the inner wall of the enzymolysis tank (1).
10. The enzymatic hydrolysis device for momordica dioica polypeptide extraction according to claim 3, wherein a pipe clamp for fixing the catheter (18) is installed on the side wall of the catheter (18), and the pipe clamp is installed on the side wall of the enzymatic hydrolysis tank (1).
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| CN202210915542.6A CN115029403B (en) | 2022-08-01 | 2022-08-01 | Mountain balsam pear polypeptide draws uses enzymolysis device |
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| CN202210915542.6A CN115029403B (en) | 2022-08-01 | 2022-08-01 | Mountain balsam pear polypeptide draws uses enzymolysis device |
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| CN115029403B CN115029403B (en) | 2023-03-10 |
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