CN115027052A - Tibial bone support and 3D printing method thereof - Google Patents
Tibial bone support and 3D printing method thereof Download PDFInfo
- Publication number
- CN115027052A CN115027052A CN202210637108.6A CN202210637108A CN115027052A CN 115027052 A CN115027052 A CN 115027052A CN 202210637108 A CN202210637108 A CN 202210637108A CN 115027052 A CN115027052 A CN 115027052A
- Authority
- CN
- China
- Prior art keywords
- circle
- printing
- nth
- layer
- tibial bone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000000988 bone and bone Anatomy 0.000 title claims abstract description 66
- 238000010146 3D printing Methods 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 105
- 210000002303 tibia Anatomy 0.000 claims abstract description 38
- 238000007639 printing Methods 0.000 claims abstract description 37
- 238000002156 mixing Methods 0.000 claims abstract description 34
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims abstract description 31
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims abstract description 31
- 230000008569 process Effects 0.000 claims abstract description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 31
- 108010010803 Gelatin Proteins 0.000 claims description 31
- 239000008273 gelatin Substances 0.000 claims description 31
- 229920000159 gelatin Polymers 0.000 claims description 31
- 235000019322 gelatine Nutrition 0.000 claims description 31
- 235000011852 gelatine desserts Nutrition 0.000 claims description 31
- 235000010413 sodium alginate Nutrition 0.000 claims description 31
- 239000000661 sodium alginate Substances 0.000 claims description 31
- 229940005550 sodium alginate Drugs 0.000 claims description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 239000002994 raw material Substances 0.000 claims description 7
- 239000011148 porous material Substances 0.000 abstract description 23
- 230000008859 change Effects 0.000 abstract description 11
- 239000000203 mixture Substances 0.000 abstract description 6
- 239000011664 nicotinic acid Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 2
- 230000001276 controlling effect Effects 0.000 description 8
- 230000007547 defect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000001125 extrusion Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 230000001054 cortical effect Effects 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 210000003275 diaphysis Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000004349 growth plate Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 208000006735 Periostitis Diseases 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037182 bone density Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 210000003460 periosteum Anatomy 0.000 description 1
- 239000012255 powdered metal Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/10—Processes of additive manufacturing
- B29C64/165—Processes of additive manufacturing using a combination of solid and fluid materials, e.g. a powder selectively bound by a liquid binder, catalyst, inhibitor or energy absorber
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2/3094—Designing or manufacturing processes
- A61F2/30942—Designing or manufacturing processes for designing or making customized prostheses, e.g. using templates, CT or NMR scans, finite-element analysis or CAD-CAM techniques
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2/38—Joints for elbows or knees
- A61F2/389—Tibial components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/10—Processes of additive manufacturing
- B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/28—Bones
- A61F2002/2892—Tibia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/30—Joints
- A61F2/3094—Designing or manufacturing processes
- A61F2002/30985—Designing or manufacturing processes using three dimensional printing [3DP]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/25—Process efficiency
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Manufacturing & Machinery (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Physics & Mathematics (AREA)
- Transplantation (AREA)
- Veterinary Medicine (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Heart & Thoracic Surgery (AREA)
- Biomedical Technology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Cardiology (AREA)
- Optics & Photonics (AREA)
- Mechanical Engineering (AREA)
- Physical Education & Sports Medicine (AREA)
- Geometry (AREA)
- Prostheses (AREA)
Abstract
The invention relates to the technical field of bionic material processing, and provides a 3D printing method of a tibial bone scaffold, which realizes the change of the composition of substances in the radial direction of a tibia by controlling the mixing ratio of a material containing hydroxyapatite and a material not containing hydroxyapatite in the 3D printing process; by adopting a curve printing path mode, the printing path is continuous and smooth, and stress concentration is reduced; each curve is defined to be formed by combining a plurality of tangent semicircles with the same diameter, and the diameter of the circle forming each curve is gradually increased along the radial direction from outside to inside, so that the pore size of the stent also has the characteristic of gradient increase along the radial direction; the wave crest of the Nth circle in each layer is limited to be opposite to the wave trough of the Nth circle in the adjacent layer, so that the adjacent layers have the functions of supporting each other and reducing pores; the finally obtained tibial bone scaffold has the characteristic that the radial material concentration and the pore structure present gradient change.
Description
Technical Field
The invention belongs to the technical field of bionic material processing, and particularly relates to a tibial bone scaffold and a 3D printing method thereof.
Background
When the damaged bone tissue is small, the bone tissue can be self-healed; however, when the bone defect reaches a certain degree, the bone defect cannot be repaired by simply relying on the self-healing capability of the bone. Filling a bone defect with a bone repair material is a common treatment protocol at present. The tibial bone scaffold is a common bone repair material, and is usually prepared by a 3D printing technology. 3D printing is a technique of constructing an object by printing layer by layer using an adhesive material such as powdered metal or plastic based on a digital model file.
Although the preparation of the tibial bone scaffold is realized by a 3D printing technology in the prior art, the bone density in the natural tibia is non-uniformly distributed and has a gradient pore structure; its porosity grow gradually from outer to interior, and the outside region is mostly the cortex bone, and its porosity is lower, and mechanical strength is higher, and the inside region is mostly the cancellous bone, and its porosity is higher, and mechanical strength is lower. Meanwhile, the natural bone is mainly formed by compounding hydroxyapatite and various collagens, wherein, the content of inorganic components such as hydroxyapatite and the like in the outer layer cortical bone is higher, thereby maintaining higher mechanical strength; in the inner cancellous bone, organic components such as collagen are contained in a higher amount, thereby maintaining activation of blood vessels and transport of nutrients. However, in the 3D printing common in the prior art, a single material and single process is mostly adopted, and the prepared tibial bone scaffold has a uniform structure and a single component; and most of the printing is in a well-shaped lattice structure, so that the prepared tibial material cannot realize the structure gradient change and the component gradient continuous change in the radial direction.
Therefore, it is desirable to provide a 3D printing method for tibial bone scaffolds that more closely resembles the natural tibia, resulting in a tibial bone scaffold with radial material concentration and gradient pore structure.
Disclosure of Invention
In view of this, the present invention provides a method for 3D printing of a tibial bone scaffold. By utilizing the 3D printing method provided by the invention, the obtained tibial bone scaffold has the characteristic that the radial material concentration and the pore structure show gradient change, and is closer to a natural tibia.
The invention provides a 3D printing method of a tibial bone scaffold, which comprises the following steps:
(1) mixing sodium alginate, gelatin and water to obtain a material A; mixing sodium alginate, gelatin, hydroxyapatite and water to obtain a material B;
(2) layering along the axial direction of the tibia according to the target tibia structure, and performing 3D printing layer by taking the material A and the material B obtained in the step (1) as raw materials for 3D printing to obtain a tibia bone support;
the path of 3D printing in each layer is:
sequentially printing a first circle, a second circle and an Nth circle from outside to inside to obtain a layer;
the first, second and nth turns are independently comprised of a plurality of tangent semi-circles;
the diameters of semicircles in the first circle, the second circle and the Nth circle are sequentially increased;
in the same circle, the diameters of all semi-circles are the same; in the same circle, the arc opening of one semicircle in two adjacent semicircles points to the circle center of the circle, and the arc opening of the other semicircle is back to the circle center of the circle, so that each circle is wavy;
in adjacent layers, the wave crest of the Nth circle of one layer is opposite to the wave trough of the Nth circle of the other layer, so that the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure;
and printing the first circle, the second circle and the Nth circle, and controlling the mixing ratio of the material A and the material B to gradually increase the proportion of the material A and gradually decrease the proportion of the material B.
Preferably, the mixing temperature of the sodium alginate, the gelatin and the water in the step (1) is 45-55 ℃.
Preferably, the mixing temperature of the sodium alginate, the gelatin, the hydroxyapatite and the water in the step (1) is 55-65 ℃.
Preferably, the mixing ratio of the material A and the material B in the step (2) is controlled by a micro pump.
The invention provides a tibial bone scaffold prepared by the preparation method in the scheme.
The invention provides a 3D printing method of a tibial bone scaffold, which comprises the following steps: (1) mixing sodium alginate, gelatin and water to obtain a material A; mixing sodium alginate, gelatin, hydroxyapatite and water to obtain a material B; (2) layering along the axial direction of the tibia according to the target tibia structure, and performing 3D printing layer by taking the material A and the material B obtained in the step (1) as raw materials for 3D printing to obtain a tibia bone support; the path of 3D printing in each layer is: sequentially printing a first circle and a second circle from outside to inside until an Nth circle to obtain a layer; the first ring, the second ring and the Nth ring are independently composed of a plurality of tangent semicircles; the diameters of semicircles in the first circle, the second circle and the Nth circle are sequentially increased; in the same circle, the diameters of all semi-circles are the same; in the same circle, the arc opening of one semicircle in two adjacent semicircles points to the circle center of the circle, and the arc opening of the other semicircle is back to the circle center of the circle, so that each circle is wavy; in adjacent layers, the wave crest of the Nth circle of one layer is opposite to the wave trough of the Nth circle of the other layer, so that the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure; and printing the first circle, the second circle and the Nth circle, and controlling the mixing ratio of the material A and the material B to gradually increase the proportion of the material A and gradually decrease the proportion of the material B. According to the invention, the change of the composition of the substance in the radial direction of the tibia is realized by controlling the mixing ratio of the material containing the hydroxyapatite and the material not containing the hydroxyapatite in the 3D printing process. By adopting a curve printing path mode, the printing path is continuous and smooth, and stress concentration is reduced; each curve is defined to be formed by combining a plurality of tangent semicircles with the same diameter, and the diameter of the circle forming each curve is gradually increased along the radial direction from outside to inside, so that the pore size of the stent also has the characteristic of gradient increase along the radial direction; by limiting the wave crest of the Nth circle of one layer to be opposite to the wave trough of the Nth circle of the other layer in adjacent layers, the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure, and the functions of mutual supporting and pore reduction are achieved. Therefore, the tibia bone scaffold obtained by the 3D printing method has the characteristic that the radial material concentration and the pore structure present gradient change, and is closer to a natural tibia.
Drawings
FIG. 1 is a schematic view of a natural tibia structure;
FIG. 2 is a simulation of the multi-layered structure and the two-layered structure of the tibial bone scaffold provided by the present invention;
FIG. 3 is a schematic view of the tibial bone scaffold provided by the present invention in terms of structure and composition in the axial direction;
FIG. 4 is a diagram of a printing path of an odd number of layers on the layer structure of a tibial bone scaffold provided by the present invention;
FIG. 5 is a diagram of a print path for an even number of layers on a layer structure of a tibial bone scaffold provided by the present invention;
fig. 6 is a device and a flowchart for 3D printing of a tibial bone scaffold provided by the present invention.
Detailed Description
The invention provides a 3D printing method of a tibial bone scaffold, which comprises the following steps:
(1) mixing sodium alginate, gelatin and water to obtain a material A; mixing sodium alginate, gelatin, hydroxyapatite and water to obtain a material B;
(2) layering along the axial direction of the tibia according to the target tibia structure, and performing 3D printing layer by taking the material A and the material B obtained in the step (1) as raw materials for 3D printing to obtain a tibia bone support;
the path of 3D printing in each layer is:
sequentially printing a first circle and a second circle from outside to inside until an Nth circle to obtain a layer;
the first, second and nth turns are independently comprised of a plurality of tangent semi-circles;
the diameters of semicircles in the first circle, the second circle and the Nth circle are sequentially increased;
in the same circle, the diameters of all semi-circles are the same; in the same circle, the arc opening of one semicircle in two adjacent semicircles points to the circle center of the circle, and the arc opening of the other semicircle is back to the circle center of the circle, so that each circle is wavy;
in adjacent layers, the wave crest of the Nth circle of one layer is opposite to the wave trough of the Nth circle of the other layer, so that the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure;
and printing the first circle, the second circle and the Nth circle, and controlling the mixing ratio of the material A and the material B to gradually increase the proportion of the material A and gradually decrease the proportion of the material B.
The method mixes sodium alginate, gelatin and water to obtain a material A.
The invention has no special regulation on the dosage relation of the sodium alginate, the gelatin and the water, and can be adjusted according to the actual requirement. In the embodiment of the invention, the relation of the using amount of the sodium alginate, the gelatin and the water is preferably 0.4g to 0.5g to 10 ml. In the invention, the material A mainly provides a component which forms the tibial bone scaffold and does not contain hydroxyapatite.
In the invention, the mixing temperature of the sodium alginate, the gelatin and the water is preferably 45-55 ℃, and more preferably 52 ℃. The invention limits the mixing temperature within the range, which is beneficial to fully and uniformly mixing the sodium alginate and the gelatin in the water.
According to the invention, sodium alginate, gelatin, hydroxyapatite and water are mixed to obtain a material B.
In the present invention, the amount of sodium alginate and gelatin in said material B is preferably the same as the amount of sodium alginate and gelatin in material a, except that a certain amount of hydroxyapatite is added. The dosage of the hydroxyapatite is not specially determined, and the hydroxyapatite is added according to actual requirements. The invention limits the dosage of the sodium alginate and the gelatin in the material B to be the same, aims to realize that the dosage relationship between the sodium alginate and the gelatin in the materials A and B is not changed, and realizes the dosage change of the hydroxyapatite in the mixture by regulating and controlling the proportion of the materials A and B. In the invention, the material B mainly provides a component containing hydroxyapatite for forming the tibial bone scaffold.
In the invention, the mixing temperature of the sodium alginate, the gelatin, the hydroxyapatite and the water is preferably 55-65 ℃, and more preferably 60 ℃. The invention limits the mixing temperature within the range, which is beneficial to fully and uniformly mixing the sodium alginate, the gelatin and the hydroxyapatite in the water.
The invention takes a material B containing hydroxyapatite and a material A without hydroxyapatite as raw materials, and realizes the change of the material composition on the tibia radial direction by controlling the mixing ratio of the materials A and B in the 3D printing process.
After the material A and the material B are obtained, layering is carried out along the axial direction of the tibia according to the target tibia structure, the material A and the material B are used as raw materials for 3D printing, and 3D printing is carried out layer by layer to obtain the tibia bone support.
The natural tibia structure is schematically shown in fig. 1, and it can be seen from fig. 1 that the tibia is composed of an epiphyseal part, a growth plate, cancellous bone, a diaphysis and a fused growth plate; wherein the diaphysis comprises cortical bone, a medullary cavity and a periosteum; the axial cross-sectional view of the tibial region taken is approximately circular.
The layering method is not particularly limited in the present invention, and may be a layering method according to 3D printing known to those skilled in the art.
According to the concentration distribution characteristics of inorganic hydroxyapatite and organic sodium alginate and gelatin in the bionic natural tibia, materials in the material feeding ports A and B are pushed to a mixing cavity of a 3D printing device through a micro pump, then two-phase materials are uniformly mixed through rotation of a screw rod of the 3D printing device and then are extruded to a low-temperature receiving platform of the 3D printing device, and a printing path is generated according to a constructed tibial bone support model to drive the platform to move, so that the bionic tibial bone support is formed.
The 3D printing device is not particularly specified in the present invention, and a 3D printing device known to those skilled in the art may be used. The control mode of the motion platform and the temperature control component for 3D printing is not specially specified, and the control mode known by the technical personnel in the field is adopted to carry out conventional control on the motion platform and the temperature control component for 3D printing.
The invention provides a simulation diagram of a multilayer structure and a two-layer structure of a tibial bone support, which is shown in figure 2. As can be seen from fig. 2, the tibial bone scaffold is axially composed of a plurality of layers, and the upper semicircle and the lower semicircle of each two adjacent layers form a round structure.
In the present invention, the path of 3D printing in each layer is: sequentially printing a first circle and a second circle from outside to inside until an Nth circle to obtain a layer; the first, second and nth turns are independently comprised of a plurality of tangent semi-circles; the diameters of semicircles in the first circle, the second circle and the Nth circle are sequentially increased; in the same circle, the diameters of all semi-circles are the same; in the same circle, the arc opening of one semicircle in two adjacent semicircles points to the circle center of the circle, and the arc opening of the other semicircle is back to the circle center of the circle, so that each circle is wavy; in adjacent layers, the wave crest of the Nth circle of one layer is opposite to the wave trough of the Nth circle of the other layer, so that the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure.
The invention provides a printing path diagram of odd layers on the layer structure of the tibial bone scaffold, which is shown in figure 4. As can be seen from fig. 4, when printing the odd layers of the tibial bone scaffold, the starting point prints the first circle from the peak of the curve, and then prints the second to fifth circles step by step, and simultaneously the circle radius of each circle gradually increases.
The present invention provides a printed path diagram for even layers on a layered structure of a tibial bone scaffold, see fig. 5. As can be seen from fig. 5, when even-numbered layers of the tibial bone scaffold are printed, the starting point is printed for the first circle from the trough of the curve, and then the second to fifth circles are printed step by step, and the circle radius of each circle is gradually increased.
The diameter of the semicircle in each circle is not specially limited, the proper diameter of the semicircle is designed to be used as the diameter of the semicircle for printing the first circle according to the diameter of the target tibia, and the diameter of the semicircle is sequentially increased to print the second circle till the Nth circle. In an embodiment of the invention, the radius of the half circle printing the first turn is preferably 0.8mm or 1.6 mm; the radius of the half circle of the last turn printed is preferably 2 mm.
According to the invention, by adopting a curve printing path mode, the printing path is continuous and smooth, and stress concentration is reduced; each curve is defined to be formed by combining a plurality of tangent semicircles with the same diameter, and the diameter of the circle forming each curve is gradually increased along the radial direction from outside to inside, so that the pore size of the stent also has the characteristic of gradient increase along the radial direction; by limiting the wave crest of the Nth circle of one layer to be opposite to the wave trough of the Nth circle of the other layer in adjacent layers, the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure, and the functions of mutual supporting and pore reduction are achieved.
In the invention, in the process from printing the first circle, the second circle to the Nth circle, the mixing ratio of the material A and the material B is controlled, so that the proportion of the material A is gradually increased, and the proportion of the material B is gradually decreased.
The structure and the components of the tibial bone bracket provided by the invention in the axial direction are schematically shown in figure 3. As can be seen from fig. 3: in the layer structure, the main components of the outer layer of the tibial bone scaffold are sodium alginate, gelatin and hydroxyapatite; the main components of the inner layer are sodium alginate and gelatin, and the proportion of the hydroxyapatite is gradually reduced from the outside to the inside.
Preferably, the material A and the material B are respectively introduced into the feeding pipe A and the feeding pipe B through a micro pump, and then introduced into a mixing cavity of a 3D printer to be mixed and printed. In the present invention, the mixing ratio of the material A and the material B is preferably controlled by a micro pump. According to the invention, the mixing ratio of the material A and the material B can be accurately controlled through the micro pump, so that the dosage of the hydroxyapatite in the material can be accurately controlled.
According to the 3D printing method of the tibial bone scaffold, provided by the invention, the tibial bone scaffold with gradually changed radial material concentration and pore structure gradient is finally obtained by adopting a mode of separately feeding inorganic materials and organic materials and matching with a curve printing path mode.
The invention provides a tibial bone scaffold prepared by the preparation method in the scheme. The content of inorganic hydroxyapatite forming the tibial bone scaffold is gradually reduced and the aperture of the pore structure forming the tibial bone scaffold is gradually increased in the radial direction from outside to inside.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. The described embodiments are only some embodiments of the invention, not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a device and a flow chart for 3D printing of a tibial bone scaffold, which are shown in figure 6: heating gelatin and sodium alginate in a water bath at 52 ℃ and stirring to obtain a material A; heating gelatin, sodium alginate and hydroxyapatite in water bath at 60 deg.C, and stirring to obtain material B; control respectively through two micropumps, make material A and material B get into feed pipe A and feed pipe B respectively, then get into the hybrid chamber through the feed pipe, stir propulsion screw rod, through control motion platform and temperature control component, at last extrusion moulding.
Example 1
Simulation printing height is 5 mm's shin bone scaffold
1. Preparation of printing Material
1-1) dissolving 0.4g of sodium alginate and 0.5g of gelatin in 10ml of deionized water under heating in a water bath at 52 ℃ to obtain a solution A and loading the solution A into a feeding tube;
1-2) 0.4g of sodium alginate, 0.5g of gelatin and 0.5g of hydroxyapatite were dissolved in 10ml of deionized water under heating in a water bath at 60 ℃ to give a solution B and loaded in a feed tube.
2. Designing a tibial bone scaffold and planning a print path
2-1) designing the appearance structure of the tibial bone bracket according to the defect position and size of the tibia to obtain a tibial bone bracket model with the height of 5 mm;
2-2) designing a pore structure of the tibial bone scaffold according to the defect position, wherein the pore diameter of the pore structure at the outermost layer is 0.8mm, and the pore diameter of the pore structure at the innermost layer is 2 mm;
2-3) designing and planning a printing path according to the tibial bone scaffold structure:
according to the target tibia structure, layering is conducted along the axial direction of the tibia, the printing material is prepared in the step 1, the material A and the material B serve as raw materials for 3D printing, and 3D printing is conducted layer by layer to obtain a tibia bone support;
the path of 3D printing in each layer is:
sequentially printing a first circle and a second circle from outside to inside until an Nth circle to obtain a layer;
the first, second and nth turns are independently comprised of a plurality of tangent semi-circles;
the diameters of semicircles in the first circle, the second circle and the Nth circle are sequentially increased;
in the same circle, the diameters of all semi-circles are the same; in the same circle, the arc opening of one semicircle in two adjacent semicircles points to the circle center of the circle, and the arc opening of the other semicircle is back to the circle center of the circle, so that each circle is wavy;
in adjacent layers, the wave crest of the Nth circle of one layer is opposite to the wave trough of the Nth circle of the other layer, so that the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure;
and printing the first circle, the second circle and the Nth circle, and controlling the mixing ratio of the material A and the material B to gradually increase the proportion of the material A and gradually decrease the proportion of the material B.
3. Printing tibia bone support
3-1) the micro-pump control system controls the speed at which the feed tube A, B is extruded into the mixer based on the print path;
a) when the outer cortical tibial bone scaffold part is printed, the feeding pipe B is controlled to extrude, the feeding pipe A does not extrude, and the concentration of HA (hydroxyapatite) in the material extruded by the nozzle is about 3.51 wt%;
b) as the printing path gradually approaches the center, the extrusion speed of the feeding pipe A is gradually increased, the extrusion speed of the feeding pipe B is gradually reduced, and meanwhile, the HA proportion in the material extruded by the nozzle is gradually reduced;
c) when the center portion was printed, the supply tube A was controlled to extrude and the supply tube B did not extrude, and the HA concentration in the head extrusion material was 0 wt%.
3-2) stirring the mixed material solution uniformly by a screw, extruding and propelling the material solution into a printing nozzle (the diameter of the nozzle is 0.2 mm);
3-3) the receiving platform moves on an X axis and a Y axis, and the moving speed of the platform is 5 mm/s;
3-4) the starting temperature of the temperature control assembly on the receiving platform is 4 ℃.
3-5) the receiving platform moves 0.2mm along the negative direction of the Z axis, the temperature of the temperature control assembly is reduced by 0.5 ℃, the step 3.1-3.3 is repeated, and a second layer is printed;
3-6) repeating the steps 3-3) to 3-5) until the printing is finished, and obtaining the tibial bone scaffold with the height of 5mm and the pore diameter of 0.8mm to 2mm and with gradually changed radial material concentration and pore structure gradient.
Example 2
A tibial bone scaffold with gradually changed radial material concentration and pore structure gradient is prepared according to the method of the embodiment 1, except that the diameter of the innermost layer hole of the tibial bone scaffold at the 1 st to 4 th layers is 1.6 mm. Due to the characteristic that the more the position of the bionic natural tibia close to a medullary cavity in the axial direction is, the less the spongy bone is, the tibia bone support with higher compression strength and gradually changed radial material concentration and pore structure gradient is obtained.
It can be seen from the above embodiments that the present invention realizes the change of the composition of the material in the radial direction of the tibia by controlling the mixing ratio of the materials a and B; the gradient gradual change of the pore structure in the axial direction of the tibia is realized by adopting a curve printing path mode.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A3D printing method of a tibial bone scaffold comprises the following steps:
(1) mixing sodium alginate, gelatin and water to obtain a material A; mixing sodium alginate, gelatin, hydroxyapatite and water to obtain a material B;
(2) layering along the axial direction of the tibia according to the target tibia structure, and performing 3D printing layer by taking the material A and the material B obtained in the step (1) as raw materials for 3D printing to obtain a tibia bone support;
the path of 3D printing in each layer is:
sequentially printing a first circle and a second circle from outside to inside until an Nth circle to obtain a layer;
the first, second and nth turns are independently comprised of a plurality of tangent semi-circles;
the diameters of semicircles in the first circle, the second circle and the Nth circle are sequentially increased;
in the same circle, the diameters of all semi-circles are the same; in the same circle, the arc opening of one semicircle in two adjacent semicircles points to the circle center of the circle, and the arc opening of the other semicircle is back to the circle center of the circle, so that each circle is wavy;
in adjacent layers, the wave crest of the Nth circle of one layer is opposite to the wave trough of the Nth circle of the other layer, so that the semi-circles of the Nth circle of the adjacent layers are spliced into a complete circle structure;
and printing the first circle, the second circle and the Nth circle, and controlling the mixing ratio of the material A and the material B to gradually increase the proportion of the material A and gradually decrease the proportion of the material B.
2. The 3D printing method according to claim 1, wherein the mixing temperature of the sodium alginate, the gelatin and the water in the step (1) is 45-55 ℃.
3. The 3D printing method according to claim 1, wherein the mixing temperature of the sodium alginate, the gelatin, the hydroxyapatite and the water in the step (1) is 55-65 ℃.
4. The 3D printing method according to claim 1, wherein the mixing ratio of the material A and the material B in the step (2) is controlled by a micro pump.
5. A tibial bone scaffold obtainable by the process of any one of claims 1 to 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210637108.6A CN115027052A (en) | 2022-06-07 | 2022-06-07 | Tibial bone support and 3D printing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210637108.6A CN115027052A (en) | 2022-06-07 | 2022-06-07 | Tibial bone support and 3D printing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115027052A true CN115027052A (en) | 2022-09-09 |
Family
ID=83123049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210637108.6A Pending CN115027052A (en) | 2022-06-07 | 2022-06-07 | Tibial bone support and 3D printing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115027052A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109809810A (en) * | 2019-03-07 | 2019-05-28 | 华南理工大学 | A kind of bioactive ceramics bracket and preparation method thereof with heterogeneous porous bionical natural bony structure |
| US20200179121A1 (en) * | 2017-05-29 | 2020-06-11 | University College Dublin, National University Of Ireland, Dublin | An implantable medical device |
| CN113172880A (en) * | 2021-05-05 | 2021-07-27 | 西北工业大学 | Continuous gradient bionic manufacturing method based on pneumatic precise control of active cartilage support |
-
2022
- 2022-06-07 CN CN202210637108.6A patent/CN115027052A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20200179121A1 (en) * | 2017-05-29 | 2020-06-11 | University College Dublin, National University Of Ireland, Dublin | An implantable medical device |
| CN109809810A (en) * | 2019-03-07 | 2019-05-28 | 华南理工大学 | A kind of bioactive ceramics bracket and preparation method thereof with heterogeneous porous bionical natural bony structure |
| CN113172880A (en) * | 2021-05-05 | 2021-07-27 | 西北工业大学 | Continuous gradient bionic manufacturing method based on pneumatic precise control of active cartilage support |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111070376B (en) | 3D printing bionic porous bioceramic artificial bone and preparation method thereof | |
| CN107823714B (en) | Forming system for preparing tissue engineering osteochondral scaffold and biological 3D printing forming method | |
| Wang et al. | A building‐block approach to 3D printing a multichannel, organ‐regenerative scaffold | |
| AU2002325762B2 (en) | Porous ceramic composite bone grafts | |
| US11534527B2 (en) | Method for supplying inks for three-dimensional printing, and three-dimensional printing method using same | |
| CN111803715B (en) | Degradable artificial bone particle with core-shell structure and preparation method thereof | |
| CN107412851B (en) | Construction method of prevascularized bulk biological bone scaffold of composite cells | |
| CN110916735B (en) | Degradable bioactive screw and manufacturing method thereof | |
| US20180112167A1 (en) | Rotary device for bio-printing and method for using the same | |
| CN113262325B (en) | 3D printing customized programmed specific biological function bone tissue repair regeneration promoting scaffold material and preparation method thereof | |
| CN104826171A (en) | Material increase manufacturing method of multi-scale biomimetic artificial bone support | |
| CN113172880B (en) | Continuous gradient bionic manufacturing method based on pneumatic precise control of active cartilage support | |
| KR102083788B1 (en) | 3D printing system for artificial blood vessel manufacturing and method of manufacturing artificial blood vessel using same | |
| CN112245077B (en) | Aperture gradient porous scaffold and minimum curved surface structural unit used for same | |
| CN103143062A (en) | Three-dimensional controllable incremental forming method and forming system for active osteochondral integrated gradient scaffold | |
| CN107898533A (en) | Manually carry the coaxial regeneration vessel stent of medicine and its combination process preparation method | |
| WO2007128192A1 (en) | A medical strengthened-type porous bioceramics, its preparation method and application | |
| Huang et al. | Preparation of multigradient hydroxyapatite scaffolds and evaluation of their osteoinduction properties | |
| CN115027052A (en) | Tibial bone support and 3D printing method thereof | |
| CN115042428A (en) | Additive manufacturing method for constructing multiple continuous gradient functional bone scaffold | |
| CN110916736A (en) | Porous degradable screw and manufacturing method thereof | |
| CN112386345B (en) | Alveolar bone increment support system | |
| CN108158697A (en) | bone defect filling bracket structure | |
| CN104606718A (en) | Preparation method of composite material bionic bone scaffold containing drug carrying microsphere | |
| CN112120833A (en) | Method for enhancing initial strength of 3D printing bone by implant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220909 |