CN115025893B - Blood low-temperature centrifuge, whole blood separating method and erythrocyte washing method - Google Patents
Blood low-temperature centrifuge, whole blood separating method and erythrocyte washing method Download PDFInfo
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- CN115025893B CN115025893B CN202210958233.7A CN202210958233A CN115025893B CN 115025893 B CN115025893 B CN 115025893B CN 202210958233 A CN202210958233 A CN 202210958233A CN 115025893 B CN115025893 B CN 115025893B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0428—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with flexible receptacles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B2005/0435—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles with adapters for centrifuge tubes or bags
Abstract
The invention is suitable for the field of medical appliances, and provides a blood low-temperature centrifuge, a whole blood separating method and a red blood cell washing method; wherein, blood low temperature centrifuge includes the organism, and the inboard of organism is equipped with centrifugal cabin, and the tip of organism is equipped with and is used for the sealed cover of centrifugal cabin, still includes: the centrifugal assembly is arranged in the centrifugal cabin, the adapter is arranged on the centrifugal assembly, and the centrifugal assembly is used for driving the adapter to rotate; the adapter comprises a bag cabin and an infusion assembly, wherein the bag cabin is used for placing the liquid bag, and the infusion assembly is used for delivering liquid to the appointed liquid bag; and the control assembly is respectively used for controlling the centrifugal assembly and the infusion assembly. The invention is convenient for the effective control of blood component centrifugation, the blood bag does not leave the centrifuge in the whole preparation process, and the invention has the advantages of high efficiency and high quality for preparing the component blood.
Description
Technical Field
The invention belongs to the field of medical appliances, and particularly relates to a blood low-temperature centrifugal machine, a whole blood separating method and a red blood cell washing method.
Background
Under the action of an appropriate centrifugal force, various blood components are layered due to the difference in specific gravity, and thus can be separated.
In the prior art, some blood components are separated by a manual method, so that the workload of an operator is large, the efficiency is low, and the separation effect difference of products is large; some adopt the machine to assist the separation, the blood bag need be taken out from centrifuge, installs on the component blood separator, and the blood bag is probably rocked in the process of taking out to can influence the blood layering, in the separation process simultaneously, blood leaves centrifugal low temperature environment, also has an influence to blood quality.
Therefore, in view of the above situation, there is an urgent need to develop a blood cryocentrifuge, a method for separating whole blood, and a method for washing red blood cells, so as to overcome the disadvantages of the current practical applications.
Disclosure of Invention
The invention aims to provide a blood low-temperature centrifugal machine, a whole blood separating method and a red blood cell washing method, and aims to solve the problems of poor blood separating quality and low efficiency of the existing separating mode.
The invention is realized in this way, a blood low temperature centrifuge, including the organism, the inboard of the said organism has centrifugal cabins, the tip of the organism has cover used for sealing to centrifugal cabin, also include: the centrifugal assembly is arranged in the centrifugal cabin, an adapter is arranged on the centrifugal assembly, and the centrifugal assembly is used for driving the adapter to rotate; the adapter comprises a bag cabin and an infusion assembly, wherein the bag cabin is used for placing the liquid bag, and the infusion assembly is used for delivering liquid to the appointed liquid bag; and the control assembly is respectively used for carrying out work control on the centrifugal assembly and the infusion assembly.
According to a further technical scheme, the number of the adapters is two, the centrifugal assembly is used for driving the two adapters to rotate simultaneously, bag cabins are arranged on the two adapters, and the infusion assembly comprises a liquid pump and a valve which are arranged on one of the adapters.
According to a further technical scheme, two adapter bodies are further provided with counterweight cabins, and the counterweight cabins are used for placing balancing objects.
Further technical scheme, the centrifugation subassembly is including rotor and motor, the motor is installed in the bottom in centrifugation cabin, and the rotor is installed to the output of motor, is equipped with the support on the rotor, and two adapters use the rotor to hang on the support as central horizontal symmetry.
Further technical scheme, centrifugal component is still including the rotor lid, rotor lid demountable installation is in the rotor and is kept away from the one end of motor, just the rotor lid is used for hiding two adapters.
According to a further technical scheme, the control assembly comprises a man-machine interaction interface, a first signal transceiver, a pipeline sensor, a PID controller and a second signal transceiver, the man-machine interaction interface is arranged on the cover, the first signal transceiver is arranged on the cavity wall of the centrifugal cabin, the first signal transceiver and the motor are electrically connected with the man-machine interaction interface, the pipeline sensor, the PID controller and the second signal transceiver are arranged on one adapter, the PID controller is electrically connected with the pipeline sensor, the liquid pump, the valve and the second signal transceiver respectively, and the second signal transceiver is in wireless communication connection with the first signal transceiver.
According to the further technical scheme, the control assembly further comprises a charging socket and a battery, the charging socket is arranged on the machine cover and electrically connected with the human-computer interaction interface, and the charging socket is used for charging the battery; one of them still be equipped with the battery cabin on the adapter, the battery is placed in the battery cabin, just battery and PID controller electric connection, the battery is used for supplying power to pipeline perceptron, liquid pump, valve, PID controller and second signal transceiver.
Another object of the present invention is to provide a method for separating whole blood using the blood cryocentrifuge, comprising the steps of:
step 1, starting a blood low-temperature centrifuge and carrying out self-inspection;
step 4, hanging the adapter on a bracket of the rotor, and sequentially covering a rotor cover and a machine cover;
and 8, opening the cover and the rotor cover in sequence, opening all valves, taking out the blood collection bag, filling red blood cell suspension in the blood collection bag, and filling plasma in the transfer bag.
Another object of the present invention is to provide a method for washing erythrocytes by using the blood cryocentrifuge, comprising the steps of:
step 1, starting a blood low-temperature centrifuge and carrying out self-checking;
step 4, placing the battery in a battery cabin, wherein the battery is electrically connected with the PID controller; placing a balancing object in the balance weight cabin, wherein the weight of the adapter for placing the sodium chloride injection bag is more than that of the adapter for placing the transfer bag by the weight of the two bags of sodium chloride injection during balancing;
and step 14, opening the machine cover and the rotor cover in sequence, opening all the valves, and taking out the transfer bag, wherein the washing red blood cells are filled in the transfer bag.
According to the invention, through the arrangement of the centrifugal component and the installation of the adapter on the centrifugal component, the centrifugal component can drive the adapter to rotate, when the adapter rotates, the transfusion component can convey blood to the designated liquid bag, and the arrangement of the control component is matched, so that the effective control of blood component centrifugation is facilitated, the blood bag does not leave the centrifuge in the whole preparation process, and the advantage of high-efficiency and high-quality component blood preparation is achieved.
Drawings
Fig. 1 is a schematic structural diagram of a blood cryocentrifuge according to an embodiment of the present invention.
Fig. 2 is a schematic top view of an adapter portion of a blood cryocentrifuge according to an embodiment of the present invention.
Fig. 3 is a schematic view of a top mounting structure of an adapter part in separating whole blood in the blood cryogenic centrifuge according to the embodiment of the present invention.
Fig. 4 is a schematic top view of an adapter part of a blood low-temperature centrifuge according to an embodiment of the present invention, when washing red blood cells.
In the figure: 1-body, 2-cover, 3-human-machine interface, 4-charging socket, 5-first signal transceiver, 6-rotor, 7-rotor cover, 8-adapter, 9-centrifugal chamber, 10-motor, 11-pipeline sensor, 12-liquid pump, 13-valve, 14-PID controller, 15-counterweight chamber, 16-bag chamber, 17-battery chamber, 18-blood collection bag, 19-transfer bag, 20-preservation liquid bag, 21-battery, 22-sodium chloride injection bag, 23-connecting pipe, 24-second signal transceiver.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
Specific implementations of the present invention are described in detail below with reference to specific embodiments.
As shown in fig. 1-2, a blood low-temperature centrifuge provided for an embodiment of the present invention includes a machine body 1, a centrifuge chamber 9 is provided inside the machine body 1, a cover 2 for sealing the centrifuge chamber 9 is provided at an end of the machine body 1, the cover 2 and the machine body 1 are preferably detachably connected, and the cover 2 for protecting the centrifuge front cover is started, so as to facilitate reliable centrifugation; further comprising:
the centrifugal assembly is arranged in the centrifugal cabin 9, the adapter 8 is arranged on the centrifugal assembly, and the centrifugal assembly is used for driving the adapter 8 to rotate; the adapter 8 comprises a bag compartment 16 and an infusion set, wherein the bag compartment 16 is used for placing a liquid bag, and the infusion set is used for delivering liquid to a specified liquid bag;
and the control assembly is respectively used for controlling the centrifugal assembly and the infusion assembly.
In the embodiment of the invention, through the arrangement of the centrifugal component and the installation of the adapter 8 on the centrifugal component, the centrifugal component can drive the adapter 8 to rotate, when the adapter 8 rotates, the transfusion component can convey blood to a designated liquid bag, and the arrangement of the control component is matched, so that the effective control of blood component centrifugation is facilitated, the blood bag does not leave the centrifuge in the whole preparation process, and the advantage of high-efficiency and high-quality blood component preparation is achieved. In addition, a refrigerating device and a temperature control system can be combined, so that the low-temperature centrifugal effect is guaranteed, and limitation is not performed.
As shown in fig. 1-4, as a preferred embodiment of the present invention, there are two adapters 8, a centrifuge module is used to drive the two adapters 8 to rotate simultaneously, there are bag chambers 16 on each of the two adapters 8, the number and structure of the bag chambers 16 are not limited, and it is sufficient to reliably place a blood collection bag 18, a transfer bag 19, a preservation solution bag 20, a sodium chloride injection bag 22, etc., the infusion module includes a liquid pump 12 and a valve 13 on one of the adapters 8, and as shown in fig. 2, there are preferably 4 valves 13.
When whole blood is separated, as shown in fig. 3, a blood collection bag 18 is placed in the bag chamber 16 of one of the adapters 8, a transfer bag 19 and a preservation solution bag 20 are placed in the bag chamber 16 of the other adapter 8, the transfer bag 19 and the preservation solution bag 20 are respectively connected with the liquid pump 12 through a pipeline, a valve 13 is respectively arranged on each of the two pipelines, and the liquid pump 12 is further connected with the blood collection bag 18 through a pipeline; the liquid pump 12 is used to transfer blood in the blood collection bag 18 to the transfer bag 19 or transfer preservation liquid in the preservation liquid bag 20 to the blood collection bag 18.
When red blood cells are washed, as shown in fig. 4, a transfer bag 19 is placed in the bag chamber 16 of one of the adapters 8, the transfer bag 19 is further connected with a red blood cell bag (not shown) through a connecting pipe 23, a preservation solution bag 20 and a sodium chloride injection bag 22 are placed in the bag chamber 16 of the other adapter 8, the preservation solution bag 20 and the sodium chloride injection bag 22 are respectively connected with the liquid pump 12 through pipelines, a valve 13 is respectively arranged on the connecting pipelines between the preservation solution bag 20 and the sodium chloride injection bag 22 and the liquid pump 12, and the liquid pump 12 is further connected with the transfer bag 19 through a pipeline; the liquid pump 12 is used for conveying the sodium chloride injection in the sodium chloride injection bag 22 to the transfer bag 19, or conveying the supernatant in the transfer bag 19 to an empty bag of the sodium chloride injection bag 22, or conveying the preservation liquid in the preservation liquid bag 20 to the transfer bag 19.
Further, two still be equipped with counter weight cabin 15 on the adapter 8, counter weight cabin 15 is used for placing of balancing thing, and the balancing thing can adopt weight etc. through the setting of counter weight cabin 15, carries out the weight balancing of two adapters 8 when being convenient for centrifugation, promotes centrifugal quality and efficiency.
As shown in fig. 1-2, as a preferred embodiment of the present invention, the centrifugal assembly includes a rotor 6 and a motor 10, the motor 10 is installed at the bottom of a centrifugal chamber 9, the rotor 6 is installed at the output end of the motor 10, a bracket is integrally formed on the rotor 6, and two adapters 8 are horizontally and symmetrically suspended on the bracket by taking the rotor 6 as the center; preferably, the structure of the bracket is not limited, and the arrangement of fig. 2 can be adopted, and the rotor 6 and the bracket are designed integrally, so that the adapter 8 can be hung on the bracket.
Further, centrifugal component is still including rotor lid 7, rotor lid 7 demountable installation is in the rotor 6 one end of keeping away from motor 10, just rotor lid 7 is used for hiding two adapters 8, covers rotor lid 7 who plays the guard action before starting the centrifugation, avoids article in it to drop when the centrifugation, promotes the reliability of work.
In the embodiment of the invention, the arrangement of the centrifugal assembly is beneficial to carrying out reliable bearing on the two adapters 8 and controlling the rotation and the centrifugation, and is stable and reliable.
As shown in fig. 1-4, as a preferred embodiment of the present invention, the control component includes a human-computer interaction interface 3, a first signal transceiver 5, a pipeline sensor 11, a PID controller 14 (Proportion-Integration-Differentiation controller) and a second signal transceiver 24, the human-computer interaction interface 3 is disposed on the cover 2, the first signal transceiver 5 is disposed on the wall of the centrifuge chamber 9, the first signal transceiver 5 and the motor 10 are electrically connected to the human-computer interaction interface 3, the pipeline sensor 11, the PID controller 14 and the second signal transceiver 24 are disposed on one of the adapters 8, the PID controller 14 is electrically connected to the pipeline sensor 11, the liquid pump 12, the valve 13 and the second signal transceiver 24, the second signal transceiver 24 is wirelessly connected to the first signal transceiver 5, the pipeline sensor 11 is a sensing device for sensing the flow of liquid in the pipeline, and the prior art is used, and the present invention is not limited; the human-computer interaction interface 3 sends out a control signal, and then the PID controller 14 controls the pipeline sensor 11, the liquid pump 12 and the valve 13 to work through wireless communication between the first signal transceiver 5 and the second signal transceiver 24.
When separating whole blood, as shown in fig. 3, a line sensor 11 is further provided in the line between the liquid pump 12 and the blood collection bag 18; the man-machine interaction interface 3 controls the motor 10 to drive the adapter 8 to rotate and centrifuge in a single direction, and when blood in the blood collection bag 18 is layered, the motor 10 stops working; opening the valve 13 on the pipeline between the transfer bag 19 and the liquid pump 12, starting the liquid pump 12, leading the upper plasma layer in the blood collection bag 18 to flow to the transfer bag 19, and when the pipeline sensor 11 detects that red blood cells pass through, closing the valve 13 on the pipeline between the transfer bag 19 and the liquid pump 12 and stopping the liquid pump 12 by the PID controller 14; then, the valve 13 on the pipeline between the liquid pump 12 and the preservation liquid bag 20 is opened, the liquid pump 12 is started, the preservation liquid in the preservation liquid bag 20 flows to the blood collection bag 18, and when the pipeline sensor 11 detects that no liquid passes through, the PID controller 14 closes the valve 13 on the pipeline between the liquid pump 12 and the preservation liquid bag 20 and stops the liquid pump 12.
When washing the red blood cells, as shown in fig. 4, a pipeline sensor 11 is further disposed on the pipeline between the transfer bag 19 and the liquid pump 12; opening a valve 13 on a pipeline between a sodium chloride injection bag 22 and the liquid pump 12, starting the liquid pump 12, enabling the sodium chloride injection in one bag of the sodium chloride injection bag 22 to flow to a transfer bag 19, and when the pipeline sensor 11 detects that no liquid passes through, closing the valve 13 on the pipeline between the sodium chloride injection bag 22 and the liquid pump 12 and stopping the liquid pump 12 by the PID controller 14; the man-machine interaction interface 3 controls the motor 10 to drive the adapter 8 to rotate forward and backward, and the red blood cells in the transfer bag 19 are shaken up; then the man-machine interaction interface 3 controls the motor 10 to drive the adapter 8 to rotate and centrifuge in a single direction, and when blood in the transfer bag 19 is layered, the motor 10 stops working; the valve 13 on the pipeline between the empty sodium chloride injection bag 22 without the sodium chloride injection and the liquid pump 12 is opened, the liquid pump 12 is started, the supernatant in the transfer bag 19 flows to the empty bag, and when the pipeline sensor 11 detects that red blood cells pass through, the PID controller 14 closes the valve 13 on the pipeline between the sodium chloride injection bag 22 and the liquid pump 12 and stops the liquid pump 12; then the valve 13 on the pipeline between the preservation liquid bag 20 and the liquid pump 12 is opened, the liquid pump 12 is started, the preservation liquid in the preservation liquid bag 20 flows to the transfer bag 19, and when the pipeline sensor 11 detects that no liquid passes through, the PID controller 14 closes the valve 13 on the pipeline between the preservation liquid bag 20 and the liquid pump 12 and stops the liquid pump 12.
Further, the control assembly further comprises a charging socket 4 and a battery 21, the charging socket 4 is arranged on the cover 2, the charging socket 4 is electrically connected with the human-computer interaction interface 3, and the charging socket 4 is used for charging the battery 21; one of the adapters 8 is further provided with a battery compartment 17, the battery 21 is placed in the battery compartment 17, the battery 21 is electrically connected with the PID controller 14, and the battery 21 is used for supplying power to the pipeline sensor 11, the liquid pump 12, the valve 13, the PID controller 14 and the second signal transceiver 24, that is, when the adapter is not in operation, the charging socket 4 charges the battery 21; in operation, battery 21 is placed in battery compartment 17 to power the various components on adapter 8; preferably, the battery 21 is a storage battery capable of being charged circularly, and the specific model is not limited; the charging structure of the charging socket 4 is not limited, and the conventional technology is adopted; the removable connection between the battery 21 and the PID controller 14 is not limited and may be made using conventional techniques.
As shown in FIG. 3, an embodiment of the present invention further provides a method for separating whole blood by using the blood cryocentrifuge, comprising the following steps:
step 1, starting a blood low-temperature centrifuge and carrying out self-checking, and realizing that a human-computer interaction interface 3 controls each electrical element of an adapter 8 through a wireless connection technology;
step 4, hanging the adapter 8 on a bracket of the rotor 6, and sequentially covering the rotor cover 7 and the machine cover 2;
and 8, opening the cover 2 and the rotor cover 7 in sequence, opening all the valves 13, taking out the blood collection bag 18, filling red blood cell suspension in the blood collection bag 18, and filling plasma in the transfer bag 19.
As shown in fig. 4, an embodiment of the present invention further provides a method for washing red blood cells, which uses the blood cryocentrifuge to wash red blood cells, comprising the following steps:
step 1, starting a blood low-temperature centrifuge, carrying out self-checking, and realizing control of each electric element of an adapter 8 by a human-computer interaction interface 3 through a wireless connection technology;
step 4, placing a battery 21 in the battery compartment 17, wherein the battery 21 is electrically connected with the PID controller 14; by placing a balancing object in the weight chamber 15, the weight of the adapter 8 for placing the sodium chloride injection bag 22 is more than the weight of the adapter 8 for placing the transfer bag 19 by the weight of two bags of sodium chloride injection during balancing;
and step 14, opening the machine cover 2 and the rotor cover 7 in sequence, opening all the valves 13, and taking out the transfer bag 19, wherein the washing red blood cells are filled in the transfer bag 19.
The embodiment of the invention provides a method for separating whole blood and a method for washing red blood cells, which are matched with the blood low-temperature centrifugal machine provided by the invention for use, the method has reasonable steps, can quickly and efficiently separate whole blood to obtain red blood cell suspension and wash red blood cells, can meet the requirement of separating large-capacity blood components, and has the advantages of high efficiency and high quality in preparing component blood.
In addition, the control, model and circuit connection of each component are not specifically limited, and can be flexibly set in practical application. The circuits, electronic components and modules referred to are well within the art of prior art and, needless to say, the present invention is not limited to software and process modifications.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is specific and detailed, but not to be understood as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent should be subject to the appended claims.
Claims (1)
1. The utility model provides a blood low temperature centrifuge, includes the organism, the inboard of organism is equipped with centrifugal cabin, and the tip of organism is equipped with the cover, its characterized in that still includes:
the centrifugal assembly is arranged in the centrifugal cabin, an adapter is arranged on the centrifugal assembly, and the centrifugal assembly is used for driving the adapter to rotate;
the adapter comprises a bag cabin and an infusion assembly, wherein the bag cabin is used for placing the liquid bag, and the infusion assembly is used for delivering liquid to the appointed liquid bag;
the control assembly is respectively used for controlling the operation of the centrifugal assembly and the infusion assembly;
the centrifugal assembly is used for driving the two adapters to rotate simultaneously, and bag cabins are arranged on the two adapters;
the infusion assembly comprises a liquid pump and a valve which are arranged on one adapter;
the two adapters are also provided with counterweight cabins, and the counterweight cabins are used for placing balancing objects;
the centrifugal assembly comprises a rotor and a motor;
the motor is arranged at the bottom of the centrifugal cabin, the rotor is arranged at the output end of the motor, the rotor is provided with a support, and the two adapters are horizontally and symmetrically hung on the support by taking the rotor as a center;
the centrifugal assembly further comprises a rotor cover;
the rotor cover is detachably arranged at one end of the rotor, which is far away from the motor, and is used for covering the two adapters;
the control component comprises a man-machine interaction interface, a first signal transceiver, a pipeline sensor, a PID controller and a second signal transceiver;
the human-computer interaction interface is arranged on the machine cover, the first signal transceiver is arranged on the cavity wall of the centrifugal cabin, and the first signal transceiver and the motor are electrically connected with the human-computer interaction interface;
the pipeline sensor, the PID controller and the second signal transceiver are all arranged on one adapter, the PID controller is respectively electrically connected with the pipeline sensor, the liquid pump, the valve and the second signal transceiver, and the second signal transceiver is in wireless communication connection with the first signal transceiver;
the control assembly also comprises a charging socket and a battery;
the charging socket is arranged on the cover and electrically connected with the human-computer interaction interface, and the charging socket is used for charging the battery;
one of the adapters is also provided with a battery cabin, the battery is placed in the battery cabin and is electrically connected with the PID controller, and the battery is used for supplying power to the pipeline sensor, the liquid pump, the valve, the PID controller and the second signal transceiver;
the blood low-temperature centrifuge is used for separating whole blood or washing red blood cells, and specifically comprises the following steps:
the method for separating the whole blood by adopting the blood low-temperature centrifuge comprises the following steps:
step 1, starting a blood low-temperature centrifuge and carrying out self-inspection;
step 2, placing the blood sampling bag in a bag cabin of one adapter, placing a transfer bag and a preservation solution bag in a bag cabin of the other adapter, wherein the transfer bag and the preservation solution bag are respectively connected with a liquid pump through a pipeline with a valve, and the liquid pump is also connected with the blood sampling bag through a pipeline with a pipeline sensor;
step 3, placing the battery in a battery cabin, wherein the battery is electrically connected with the PID controller; balancing the weight of the two adapters by placing a balancing object in the balancing cabin;
step 4, hanging the adapter on a bracket of the rotor, and sequentially covering a rotor cover and a machine cover;
step 5, the man-machine interaction interface controls the motor to work, the centrifugation is started, and the blood in the blood sampling bag is layered;
step 6, the human-computer interaction interface controls the motor to stop working, the centrifugation is stopped, a valve on a pipeline between the transfer bag and the liquid pump is opened, then the liquid pump is started, and upper plasma in the blood collection bag flows to the transfer bag; when the pipeline sensor detects that red blood cells pass through, the PID controller closes a valve on a pipeline between the transfer bag and the liquid pump and controls the liquid pump to stop working;
step 7, opening a valve on a pipeline between the preservation solution bag and the liquid pump, then starting the liquid pump, and enabling the preservation solution in the preservation solution bag to flow to the blood collection bag; when the pipeline sensor detects that no liquid passes through, the PID controller closes a valve on a pipeline between the liquid storage bag and the liquid pump and controls the liquid pump to stop working;
step 8, opening the cover and the rotor cover in sequence, opening all valves, taking out the blood collection bag, filling the blood collection bag with erythrocyte suspension, and filling the transfer bag with plasma;
the method for washing the red blood cells by adopting the blood low-temperature centrifuge comprises the following steps:
step 1, starting a blood low-temperature centrifuge and carrying out self-inspection;
step 2, the erythrocyte blood bag is connected with the transfer bag through a connecting pipe, and the erythrocytes to be washed are transferred to the transfer bag;
step 3, placing the transfer bag filled with the red blood cells in a bag cabin of one adapter, placing the preservation solution bag and the sodium chloride injection bag in a bag cabin of the other adapter, wherein the preservation solution bag and the sodium chloride injection bag are respectively connected with a liquid pump through a pipeline with a valve, and the liquid pump is also connected with the transfer bag through a pipeline with a pipeline sensor;
step 4, placing a battery in a battery cabin, wherein the battery is electrically connected with the PID controller; placing a balancing object in the balancing cabin, wherein the weight of the adapter for placing the sodium chloride injection bag is more than that of the adapter for placing the transfer bag by the weight of two bags of sodium chloride injection during balancing;
step 5, hanging the adapter on a bracket of the rotor, and sequentially covering a rotor cover and a machine cover;
step 6, opening a valve on a pipeline between the sodium chloride injection bag and the liquid pump, then starting the liquid pump, and enabling the sodium chloride injection in the sodium chloride injection bag to flow to the transfer bag; when the pipeline sensor detects that no liquid passes through, the PID controller closes a valve on a pipeline between the sodium chloride injection bag and the liquid pump and controls the liquid pump to stop working, and at the moment, the weight of the two adapters is the same;
step 7, controlling the motor to rotate forwards and backwards through the human-computer interaction interface, and shaking up the red blood cells in the transfer bag;
step 8, controlling the motor to work in a single direction by the human-computer interaction interface, starting centrifugation, and layering blood in the transfer bag;
step 9, stopping centrifugation, opening a valve on a pipeline between the sodium chloride injection bag of the empty bag and the liquid pump, then starting the liquid pump, transferring the supernatant in the bag to flow to the sodium chloride injection bag, when the transfer time is the same as that in step 6, closing the valve on the pipeline between the sodium chloride injection bag and the liquid pump by the PID controller, and controlling the liquid pump to stop working, wherein the amount of the transferred liquid is the amount of one bag of sodium chloride injection;
step 10, repeating the step 6 to the step 9, and using the sodium chloride injection in the second bag of sodium chloride injection to wash the red blood cells;
step 11, repeating the step 6 to the step 8, and using the sodium chloride injection in the third bag of sodium chloride injection to wash the red blood cells;
step 12, stopping centrifugation, opening a valve on a pipeline between a sodium chloride injection bag without sodium chloride injection and a liquid pump, then starting the liquid pump, and transferring supernatant liquid in the bag to flow into an empty sodium chloride injection bag; when the pipeline sensor detects that red blood cells pass through, the PID controller controls a valve on a pipeline between a sodium chloride injection bag without sodium chloride injection and the liquid pump to be closed, and controls the liquid pump to stop working;
step 13, opening a valve on a pipeline between the preservation liquid bag and the liquid pump, then starting the liquid pump, and enabling the preservation liquid in the preservation liquid bag to flow to the transfer bag; when the pipeline sensor detects that no liquid passes through, the PID controller controls a valve on a pipeline between the liquid storage bag and the liquid pump to be closed, and controls the liquid pump to stop working;
and step 14, opening the machine cover and the rotor cover in sequence, opening all the valves, and taking out the transfer bag, wherein the washing red blood cells are filled in the transfer bag.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210958233.7A CN115025893B (en) | 2022-08-11 | 2022-08-11 | Blood low-temperature centrifuge, whole blood separating method and erythrocyte washing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210958233.7A CN115025893B (en) | 2022-08-11 | 2022-08-11 | Blood low-temperature centrifuge, whole blood separating method and erythrocyte washing method |
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| CN115025893B true CN115025893B (en) | 2022-11-04 |
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Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19846535C2 (en) * | 1998-10-09 | 2000-12-07 | Westfalia Separator Ag | centrifuge |
| JP5175184B2 (en) * | 2005-06-22 | 2013-04-03 | テルモ ビーシーティー、インコーポレーテッド | Apparatus and method for separating discrete volumes of composite liquid |
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