CN115011514A - Humic acid degrading bacteria and application thereof - Google Patents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a humic acid degrading bacterium, which is a Bacillus litoralis HA-X1 strain, is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC 24268. The invention also provides application of the bacteria in degradation of humic acid. The Bacillus seashore HA-X1CGMCC24268 strain can effectively degrade humic acid in wastewater. When the addition amount of the humic acid degrading bacteria liquid is 4% by volume and the pH value is 8, the effect of degrading the humic acid is optimal.
Description
Technical Field
The invention relates to a humic acid degrading bacterium and application thereof.
Background
Humic acid is a high molecular natural organic matter widely existing in natural environments such as soil, rivers, lakes, swamps and forests, is mainly generated by animal and plant debris, sludge and microbial flora through a series of complex humification reactions, and can account for more than 50% of the total amount of natural organic matter in natural water. Humic acid presents dark brown in water body and affects the sensory performance of the water body, the structure of the compound contains a large amount of quinone group, carbonyl group, amino group, carboxyl group, methoxyl group and other groups, the groups contain a large amount of sites which can be attacked by an oxidant, and the groups are easily attacked by chlorine to generate disinfection byproducts in the chlorination disinfection process, the disinfection byproducts usually have the effects of carcinogenesis, mutagenesis and the like, and due to the existence of the active groups, the hydrophilicity and the adsorption performance of the humic acid are improved, so that the humic acid is the basis for the reaction with metal ions and other organic matters and generates pollutants with higher toxicity, thereby forming great threat to the ecological environment and the health of human beings. Humic acid long-chain organic chromophore complex formation, which is usually black, gray brown or brown yellow, etc. The discharge of the colored wastewater not only causes visual discomfort for people, but also even causes serious pollution once entering natural water or farmlands, so that the self-purification function of the water is reduced, and the ecological environment is damaged.
The existing humic acid treatment method has physical, chemical and biological methods, and microbial degradation has the unique advantages of low cost, high efficiency, no secondary pollution and the like, and meanwhile, microbial degradation of organic matters also has the advantages of wide sources, convenience in selection, simplicity in operation and the like. Therefore, the humic acid high-efficiency degrading bacteria are separated and screened in nature, and the method has important practical significance for discussing factors such as initial concentration of humic acid, pH, inoculation amount and the like which influence the degrading performance of the high-efficiency humic acid degrading bacteria, and provides a foundation for subsequent treatment.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a humic acid degrading bacterium and application thereof.
The invention provides a humic acid degrading bacterium, which is a Bacillus maritima strain Bacillus litoralisHA-X1, and is preserved in China general microbiological culture Collection center (CGMCC) in 2022, 1 month and 10 days, wherein the preservation address is as follows: the number of the collection is CGMCC24268, No. 3 of Xilu No. 1 of Beijing Chaoyang district.
The invention also provides a humic acid degrading bacterial liquid which is obtained by carrying out amplification culture on the Haibacillus hamiltonii HA-X1 strain.
Preferably, the expanding culture is specifically as follows: inoculating the Bacillus bikedfolius HA-X1 strain into beef extract peptone liquid medium, and shake-culturing at 30 deg.C for 18-30 h.
The invention also provides application of the humic acid degrading bacteria or the humic acid degrading bacteria liquid in preparation of products for degrading humic acid.
Preferably, when humic acid is degraded, the humic acid degrading bacteria liquid is added into the humic acid-containing material for degradation.
Preferably, the degradation temperature is 30 ℃.
Preferably, when humic acid is degraded, the addition amount of the humic acid-degrading bacterial liquid is 3-5% by volume.
Preferably, when humic acid is degraded, the addition amount of the humic acid-degrading bacterial liquid is 4% by volume.
Preferably, the initial pH is 7 to 8 when humic acid is degraded.
The Bacillus hamiltonii HA-X1CGMCC24268 strain can effectively degrade humic acid in wastewater. When the addition amount of the humic acid degradation bacterial liquid is 4% by volume and the pH value is 8, the effect of humic acid degradation is optimal.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 shows the colony morphology of the strain HA-X1 on beef extract peptone solid medium.
FIG. 2 is a scanning electron micrograph of strain HA-X1.
FIG. 3 is a phylogenetic tree of strain HA-X1.
FIG. 4 is a growth and degradation curve of strain HA-X1.
FIG. 5 shows the effect of initial concentration of humic acid on the degradation performance of humic acid.
FIG. 6 is a graph showing the effect of initial pH on humic acid degradation performance.
FIG. 7 shows the effect of the bacterial liquid dosage on the degradation performance of humic acid.
Detailed Description
The following examples are intended to facilitate a better understanding of the invention, but are not intended to limit the invention thereto. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples are commercially available unless otherwise specified.
Example 1
1 materials and methods
1.1 sampling
Soil samples were collected from soil and artificial lake bottom mud 5cm below rotten wood in peng jia school zone of university of physical engineering in Lanzhou.
1.2 culture Medium
(1) Enrichment culture medium: beef extract peptone medium: 5g of beef extract, 10g of peptone, 5g of NaCl, 1L of distilled water and 20g of agar, wherein the pH value is 7.0-7.2.
(2) Domestication culture medium:
domestication culture medium (i): 0.5g of beef extract, 1g of peptone, 0.5g of NaCl0.5g, 0.005g of humic acid, 1L of distilled water and 20g of agar, wherein the pH value is 7.0-7.2;
acclimatization culture medium (II): 0.1g of beef extract, 0.1g of peptone, 0.05g of NaCl0.05g, 0.015g of humic acid, 1L of distilled water and 20g of agar, wherein the pH value is 7.0-7.2.
(3) Primary screening of culture medium: humic acid 0.03g, distilled water 1L, agar 20g, pH 7.0-7.2.
1.3 preparation of humic acid solution
Weighing 1g of humic acid, adding the humic acid into 900mL of deionized water, fully stirring to completely dissolve the humic acid, then adding the deionized water to dilute the humic acid to 1L, adjusting the pH value to 7, and storing the humic acid in a brown bottle for later use.
1.4 Experimental instruments and Equipment
An electronic balance; a constant temperature gas bath oscillator; a high-pressure steam sterilization pot; an electric heating constant temperature incubator; an ultraviolet-visible spectrophotometer; an optical microscope; a refrigerator; a pH meter; a scanning electron microscope; a hotplate can be debugged; an aseptic worktop; portable colorimeter.
1.5 selection of soil dilution
Taking 10g of the collected soil sample, placing the soil sample into a conical flask filled with 90mL of water, then adding 2-3 glass beads, shaking the soil sample at 30 ℃ for 1 hour by a shaking table at 150r/min, standing the soil sample for 20min, and taking supernatant, namely 10 -1 Diluting the soil suspension to 10 -2 、10 -3 、10 -4 、10 -5 Then sucking 100 mu L of bacterial liquid of each dilution, respectively coating on enrichment medium flat plates, setting 3 parallel samples for each dilution, culturing at 30 ℃ for 24h, and finding 10 times of experiments -3 Each colony in the culture medium is distributed more evenly under dilution, and a single colony is easy to pick out, so that 10 colonies are selected -3 And (5) performing subsequent bacteria screening experiments on the diluted soil suspension.
1.6 preliminary screening of humic acid degrading bacteria
Will 10 -3 Separating and purifying the bacteria on the diluted enrichment culture medium to obtain 20 strains of bacteria, inoculating the separated and purified strains in a domestication culture medium, culturing for a certain time at 30 ℃, transferring grown colonies to a domestication culture medium II, and performing gradient domestication. After the bacterial colony grows out in the domesticated culture medium II, transferring the bacterial colony into a primary screening culture medium for transferring, screening and culturing,3 strains of humic acid degrading bacteria with larger bacterial colonies are obtained by preliminary screening, and are numbered as HA-X1, HA-X2 and HA-X3 in sequence.
1.7 rescreening of humic acid degrading bacteria
Respectively preparing 3 humic acid degrading bacteria HA-X1, HA-X2 and HA-X3 into bacterial suspension with OD600 of 0.6, adding 2% of humic acid wastewater with initial concentration of 25mg/L and initial pH of 7 according to volume ratio, carrying out shake cultivation at 30 ℃ at 150r/min, setting a control group, carrying out shake cultivation on three parallel samples, and measuring UV (ultraviolet) of the samples every 24 hours 254 (UV 254 Which represents the absorption value of organic pollutants such as aromatic hydrocarbons in water at the wavelength of 254nm of ultraviolet light, and these compounds are usually the main components of natural organic compounds with complex structures, and the concentration of these compounds can be indirectly represented by the absorbance at this wavelength). The degradation rates of different humic acid degrading bacteria on humic acid are shown in Table 1. The strain HA-X1 with the highest degradation rate is selected from 3 humic acid degrading bacteria, and the degradation rate is 40.69%.
TABLE 1 degradation rates of humic acid by different humic acid-degrading bacteria
| Strain of bacillus | HA-X1 | HA-X2 | HA-X3 |
| Percent of degradation/%) | 40.69 | 28.21 | 31.17 |
1.8 preparation of bacterial liquid
Inoculating the strain HA-X1 in the step 1.6 into a beef extract peptone liquid culture medium, performing shake culture at 30 ℃, adding a fresh culture into sterile physiological saline after 24 hours, and diluting to 10 DEG 8 CFU·mL -1 And storing in a refrigerator at 4 ℃ for later use. 1.9 morphological observation and identification of humic acid degrading fungi
1.9.1 morphological observation
Carrying out streak culture on the screened humic acid degrading bacteria HA-X1 on a beef extract peptone culture medium, culturing for 24h at 30 ℃, and observing the colony morphology; and observing the characteristics of the thallus under a scanning electron microscope.
As can be seen from the morphological observation of FIG. 1, after HA-X1 is cultured on beef extract peptone medium for 24 hours, the colony is convex, is off-white, HAs neat edge and HAs wet surface; as shown in fig. 2, is rod-shaped under a scanning electron microscope.
FIG. 1 shows the colony morphology of the strain HA-X1 on beef extract peptone solid medium.
FIG. 2 is a scanning electron micrograph of strain HA-X1.
1.9.2 identification
By performing 16SrDNA sequencing on HA-X1, the strain HA-X1 HAs the highest similarity with Bacillus sp.W25(2008), and the homology is 99.65% after head and tail redundant sequences are removed, but the strain only HAs a generic name. The second highest ranked similarity was BacillusalitoralisstrainaKUDC 1714, which has a homology of 99.51%. Based on the colony morphology and the thallus characteristics, HA-X1 is identified as the Bacilluspension. The strain HA-X1 is preserved in China general microbiological culture Collection center (CGMCC) at 1 month and 10 days of 2022, the preservation address is No. 3 of Xilu No. 1 of Beijing Kogyo and the preservation number is CGMCC 24268.
FIG. 3 is a phylogenetic tree of strain HA-X1.
The 16SrDNA gene sequence of HA-X1 is as follows:
TATAATGCAGTCGAGCGAATCAATGGGAGCTTGCTCCCTGAGATTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGATTGGGATAACTCCGGGAAACCGGAGCTAATACCGGATAACATTTTGAACCGCATGGTTCAAAATTGAAAGATGGCTTCTGCTATCACTTACAGATGGACCCGCGGCGCAATAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAACAACGCCGCGTGAACGATGAAGGCCTTCGGGTCGTAAAGTTCTGTTGTTAGGGAAGAACAAGTACCAGAGTAACTGCTGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGTACGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTACAGAAGAAGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGTACGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGAGGGTTTCCGCCCTTTAGTGCTGCAGCAAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCACTGCCCGGTATAGAGATATACCTTTCCCTTCGGGGACAGTGGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTTAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAAAGGGCTGCAAGACCGCGAGGTCAAGCCAATCCCATAAAACCATTCTCAGTTCGGATTGCAGGCTGCAACTCGCCTGCATGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCGTAAGGAGCCAGCCGCC。
2 growth and degradation characteristics of humic acid degrading bacteria, namely, seashore bacillus CGMCC24268
2.1 degradation experiment of humic acid degrading fungi Bacillus seashore CGMCC24268
2.1.1 humic acid degrading bacterium Bacillus seashore CGMCC24268 growth degradation curve
Respectively adding 2% volume ratio of humic acid degrading bacteria HA-X1 bacterial liquid into humic acid solution, setting three parallel samples, setting a blank control of non-inoculated bacteria, and carrying out shake cultivation at 30 ℃ and 150 r/min. 2mL samples were taken at 0h, 4h, 8h, 12h, 16h, 20h, 24h, 36h, 48h, 60h and 72h, OD600 values were determined, and UV254 was measured every 24h to calculate degradation rates. And drawing a growth degradation curve of the strain HA-X1 in the humic acid solution by taking the culture time as a horizontal coordinate and the OD600 value and the humic acid degradation rate as vertical coordinates.
2.1.2 initial humic acid concentration
Respectively preparing humic acid wastewater with the concentration of 5mg/L, 15mg/L, 25mg/L, 35mg/L, 45mg/L and 55mg/L, adjusting the pH to 7, respectively adding 2% by volume of Bacillus hamellalis CGMCC24268 bacterial liquid, carrying out shake culture at 30 ℃ and 150r/min for 72 hours to measure the UV of the bacillus hamellae 254 、VIS 400 And chroma.
2.1.3 initial pH
Preparing humic acid wastewater with initial concentration of 25mg/L and initial pH of 3, 4, 5, 6, 7, 8, 9 and 10, respectively, adding 2% by volume of Bacillus seashore CGMCC24268 bacterial solution, culturing at 30 deg.C and 150r/min in a shaking table, and measuring UV for 72 hr 254 、VIS 400 And chroma.
2.1.4 bacterial liquid addition
Preparing humic acid wastewater with initial concentration of 25mg/L and initial pH of 8, respectively adding 0.5%, 1%, 2%, 3%, 4%, 5%, 6% by volume of Bacillus hamiltonii CGMCC24268 bacterial solution, culturing at 30 deg.C and 150r/min in a shaking table, measuring UV for 72 hr 254 、VIS 400 And chroma.
2.2 degradation experiment results of humic acid degradation fungus Bacillus seashore CGMCC24268
2.2.1 humic acid high-efficiency degrading bacteria growth degradation curve
As shown in FIG. 4, the growth curve of the humic acid highly effective degrading bacteria HA-X1 is S-shaped, which conforms to the growth rule of microorganism. The strain HA-X1 is continuously self-regulated in the initial 12h to adapt to a new environment and is in a slow period; the humic acid shows exponential growth within 12-48h and enters a logarithmic phase of growth, so that the degradation rate of the bacterial strain HA-X1 to the humic acid is increased; and after 48 hours, the humic acid tends to be stable, the newly increased number and the death number of the cells are in dynamic balance at the time of entering a stable period, the degradation rate of the humic acid by HA-X1 also tends to be stable, and the highest degradation rates are 40.69% and 44.59% respectively.
FIG. 4 is a growth and degradation curve of strain HA-X1.
2.2.2 initial humic acid concentration
As shown in FIG. 5, when the initial concentration of humic acid is 25mg/L, the degradation rate of HA-X1 to humic acid is the highest, and UV is 254 、VIS 400 (UV-visible spectrophotometry with ultrapure water as blank control, and measurement of absorbance value at 400nm of sample solution VIS 400 Is used for representing the content of chromogenic group in the complex organic matter), the removal rate of chroma and COD are 40.39%, 47.99%, 58.95% and 44.83% respectively.
FIG. 5 shows the effect of initial concentration of humic acid on the degradation performance of humic acid.
2.2.3 initial pH
As shown in FIG. 6, UV under acidic conditions at initial pH 254 、VIS 400 The removal rate of color and chroma was high, but the removal rate of TCOD (Total chemical oxygen demand) was low, and it was found that UV was present at an acidic pH 254 、VIS 400 The high removal rate of the color and the color is caused by precipitation of humic acid under an acidic condition, and is not the degradation effect of the strain HA-X1 on the humic acid. And at an initial pH of 8, UV 254 、VIS 400 The removal rate of the color and the chroma are the highest, and are respectively 44.20%, 52.22% and 61.9%; the removal of TCOD at an initial pH of 7 reached a maximum of 52.36%. As can be seen from the figure, the initial pH values of 7 and 8 were found to be the same as each other, and the TCOD removal rate was substantially unchanged, so that the optimum pH for growth of the strain HA-X1 was 8.
FIG. 6 is a graph showing the effect of initial pH on humic acid degradation performance.
2.2.4 bacterial liquid addition
As shown in FIG. 7, when the bacterial liquid dosage is 4% by volume, the degradation rate of HA-X1 to humic acid is the highest, and UV is 254 、VIS 400 The removal rates of chroma and COD were 50.05%, 58.93%, 68.52% and 56.87%, respectively.
FIG. 7 shows the effect of the bacterial liquid dosage on the degradation performance of humic acid.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> university of Rituo-Risk of Lanzhou
<120> humic acid degrading bacterium and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1423
<212> DNA
<213> Bacilluspension (Bacillus litoralis)
<400> 1
tataatgcag tcgagcgaat caatgggagc ttgctccctg agattagcgg cggacgggtg 60
agtaacacgt gggtaacctg cctgtaagat tgggataact ccgggaaacc ggagctaata 120
ccggataaca ttttgaaccg catggttcaa aattgaaaga tggcttctgc tatcacttac 180
agatggaccc gcggcgcaat agctagttgg tgaggtaacg gctcaccaag gcaacgatgc 240
gtagccgacc tgagagggtg atcggccaca ctgggactga gacacggccc agactcctac 300
gggaggcagc agtagggaat cttccgcaat ggacgaaagt ctgacggaac aacgccgcgt 360
gaacgatgaa ggccttcggg tcgtaaagtt ctgttgttag ggaagaacaa gtaccagagt 420
aactgctggt accttgacgg tacctaacca gaaagccacg gctaactacg tgccagcagc 480
cgcggtaata cgtaggtggc aagcgttgtc cggaattatt gggcgtaaag cgtacgcagg 540
cggtttctta agtctgatgt gaaagcccac ggctcaaccg tggagggtca ttggaaactg 600
gggaacttga gtacagaaga agagagtgga attccacgtg tagcggtgaa atgcgtagag 660
atgtggagga acaccagtgg cgaaggcgac tctctggtct gtaactgacg ctgaggtacg 720
aaagcgtggg gagcgaacag gattagatac cctggtagtc cacgccgtaa acgatgagtg 780
ctaagtgtta gagggtttcc gccctttagt gctgcagcaa acgcattaag cactccgcct 840
ggggagtacg gtcgcaagac tgaaactcaa aggaattgac gggggcccgc acaagcggtg 900
gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga catcccactg 960
cccggtatag agatatacct ttcccttcgg ggacagtggt gacaggtggt gcatggttgt 1020
cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttgatctt 1080
agttgccagc atttagttgg gcactctaag gtgactgccg gtgacaaacc ggaggaaggt 1140
ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatgga 1200
tggtacaaag ggctgcaaga ccgcgaggtc aagccaatcc cataaaacca ttctcagttc 1260
ggattgcagg ctgcaactcg cctgcatgaa gctggaatcg ctagtaatcg cggatcagca 1320
tgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg 1380
taacacccga agtcggtggg gtaaccgtaa ggagccagcc gcc 1423
Claims (9)
1. A humic acid degrading bacterium is characterized in that: the humic acid degrading bacteria are Bacillus litoralis HA-X1 strains of seashore, are preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and have the preservation number of CGMCC 24268.
2. Humic acid degradation bacterial liquid, which is characterized in that: is obtained by expanding and culturing the Haibacillus hamiltonii HA-X1 strain.
3. The humic acid-degrading bacterial liquid according to claim 2, wherein: the expanded culture specifically comprises the following steps: inoculating the Bacillus bikedfolius HA-X1 strain into beef extract peptone liquid medium, and shake-culturing at 30 deg.C for 18-30 h.
4. Use of the humic acid-degrading bacteria of claim 1 or the humic acid-degrading bacteria solution of claim 2 in the preparation of products for degrading humic acid.
5. Use according to claim 4, characterized in that: and when the humic acid is degraded, adding the humic acid degrading bacteria liquid into the material containing the humic acid for degradation.
6. Use according to claim 5, characterized in that: the degradation temperature was 30 ℃.
7. Use according to any one of claims 4 to 6, characterized in that: when humic acid is degraded, the addition amount of the humic acid degrading bacteria liquid is 3-5% by volume.
8. Use according to any one of claims 7, characterized in that: when humic acid is degraded, the addition amount of the humic acid degrading bacteria liquid is 4% by volume.
9. Use according to any one of claims 4 to 6, characterized in that: in the degradation of humic acid, the initial pH is 7-8.
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| CN103468598A (en) * | 2012-11-07 | 2013-12-25 | 上海大学 | Humic acid degrading strain, and screening method and application method thereof |
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| E. CATIER ESHAM, ET AL.: "Identification and characterization of humic substances-degrading bacterial isolates from an estuarine environment", FEMS MICROBIOLOGY ECOLOGY, vol. 34, no. 2 * |
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| CN115433692A (en) * | 2022-06-10 | 2022-12-06 | 兰州理工大学 | Humic acid degrading bacteria and application thereof |
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