CN103468598A - Humic acid degrading strain, and screening method and application method thereof - Google Patents
Humic acid degrading strain, and screening method and application method thereof Download PDFInfo
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- CN103468598A CN103468598A CN2012104403271A CN201210440327A CN103468598A CN 103468598 A CN103468598 A CN 103468598A CN 2012104403271 A CN2012104403271 A CN 2012104403271A CN 201210440327 A CN201210440327 A CN 201210440327A CN 103468598 A CN103468598 A CN 103468598A
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Abstract
The invention discloses a humic acid degrading strain. The strain is separated from a bioactive carbon filter tank of a water plant. The humic acid degrading strain is an Acidovorax sp., has a preservation number of CGMCC No.6564, and is preserved in China General Microbiological Culture Collection Centeron Sep. 20, 2012. The humic acid degrading strain can be used in the treatment of micro-polluted water containing humic acid. A treatment method comprises the following steps: picking the bacterium colony, carrying out amplification culture, processing to prepare a bacterium suspension, adjusting the OD 600 to 0.6, and inoculating into the humic acid micro-polluted water according to a volume ratio of 1%. The COD removal rate and the UV254 removal rate of the micro-polluted water having a humic acid initial concentration of 15mg/L by the strain are 33.8% and 48.1% respectively. Results of researches on the influences of the degradation performance of the strain by three factors comprising the humic acid initial concentration, the pH value and the temperature show that when the humic acid initial concentration is 20mg/L and the reaction temperature is 35DEG C, the strain can well perform its degradation performance on humic acid; and it is in favor of the growth of the strain when the micro-polluted water is acidic, so the degradation of the humic acid by the strain is promoted.
Description
Technical field
The present invention relates to a kind of screening and application method of humic acid degrading bacterial strain, described bacterial strain can be applicable to the processing containing the micro-polluted water of humic acids, belongs to and utilizes the technical field of microorganism to waste water control.
Background technology
Natural organic matter (natrual organic matter) is a class ubiquitous organic substance in open water supply, mainly comprises soil ulmin, amino acid, carbohydrate etc.Wherein humus content in surface pond is very high, is removal object main in drinking water treatment.The form of soil ulmin in water mainly is divided into humic acids and fulvic acid.Humic acids (humic acid) is the larger molecular organics matter of a class formation complexity, and it contains multiple functional group, as phenolic hydroxyl group, carboxyl, ketone type carbonyl etc.In general source water, content is the 10mg/L left and right, accounts for the 50%-90% of organic substance ratio in water.Humic acids very easily forms disinfection byproduct (DBP) and haloform in the Chlorine process, in some region, causes Kaschin-Beck disease, and in addition, the existence of humic acid substance can make Micropollutants generation chemical property in water change.Humic acid material also can stimulate the growth of pathogenic micro-organism.Therefore its existence in water is the great difficult problem in drinking water treatment.
At present, the removal method about humic acids is mainly the physics and chemistry method.The microorganism that utilizes nature to exist carries out biological degradation to hardly degraded organic substances such as humic acidss, has the irreplaceable advantage of other method.A large amount of data findings show, the negative impact that microorganism is caused environment the degraded of organic pollutant is little.Therefore for this target contaminant of humic acids, screening obtains the microorganism of efficient degradation, and studies the factor that affects its degradation property, and tool is of great significance.
Summary of the invention
The object of the invention is to: [1] provides a kind of humic acid degrading bacterial strain; [2] provide a kind of screening method for described humic acid degrading bacterium; [3] provide the application method of described humic acid degrading bacterium.
Humic acid degrading bacterium provided by the present invention, on the biological activated carbon gathered in certain waterworks biological activated carbon filter, separation screening obtains.This bacterial strain be a strain acidovorax facilis (
acidovoraxsp.), the bacterial strain preserving number is: CGMCC No.6564, on September 20th, 2012 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms.
Described humic acid degradation bacteria, is characterized in that this bacterial strain, on the domestication substratum, cultivates 7 days under 30 ℃, and the bacterium colony projection is smooth glossy, is cream-coloured.It is shaft-like observing its form under scanning electronic microscope.
Screening method provided by the invention, is characterized in that, comprises the following steps:
(1) isolate the different bacterium of growthhabit from the biological activated carbon collected;
(2) bacterium separation obtained carries out gradient by the domestication substratum I-III containing the different concns humic acids and cultivates domestication;
Domestication substratum I: extractum carnis 0.1g, peptone 0.2g, sodium-chlor 0.1g, humic acids 0.005g, agar 20g, pH 7.0, distilled water 1000mL; Domestication substratum II: extractum carnis 0.01g, peptone 0.02g, sodium-chlor 0.01g, humic acids 0.010g, agar 20g, pH 7.0, distilled water 1000mL; Domestication substratum III: humic acids 0.015g, agar 20g, pH 7.0, distilled water 1000mL.The bacterium that separation is obtained is cultivated by the gradient switching of domestication substratum I-III, makes bacterium be tamed and then adapt to the poor nutrient environment of humic acids existence.Using the growing state of bacterium on domestication substratum III as the primary dcreening operation foundation, and screening obtains 5 kinds of fast growths, bacterial strain that lawn is large and intensive, and number consecutively is F1, F2, F3, F4, F5.
(3) above-mentioned 5 strain bacteriums are added in the humic acids micro-polluted water, with COD
mn, UV
254for evaluation index, estimate the degradation property of bacterium to humic acids, further filter out the humic acid degrading bacterium with better degradation effect.
Application method provided by the invention is that described bacterial strain is applied to the processing containing the micro-polluted water of humic acids; Treatment process is: this bacterium colony of picking, make bacteria suspension after enlarged culturing, regulate OD600(optical density (OD) 600) and be 0.6, be 1% ratio is inoculated in the humic acids micro-polluted water by volume, measure COD, UV
254; The clearance of the micro-polluted water COD that humic acid degrading bacterium in the present invention is 15mg/L to the humic acids starting point concentration is 33.8%, UV
254clearance be 48.1%; Humic acid degrading bacterium in the present invention is 20mg/L at the humic acids starting point concentration, when temperature of reaction is 35 ℃, and the humic acids of degrading better when micro-polluted water is acidity.
The accompanying drawing explanation
Fig. 1 is the clearances of the 5 strain bacteriums that obtain of primary dcreening operation to water sample COD.
Fig. 2 is that the 5 strain bacteriums that obtain of primary dcreening operation are to water sample UV
254clearance.
The SEM scanning electron microscope (SEM) photograph that Fig. 3 is the humic acid degrading bacterium.
Fig. 4 is the impact of humic acids starting point concentration on humic acid degrading bacterium degradation property.
Fig. 5 is the impacts of different pH on humic acid degrading bacterium degradation property.
Fig. 6 is the impact of differing temps on humic acid degrading bacterium degradation property.
Embodiment
Now specific embodiments of the invention are described below.
One, a kind of screening method of humic acid degrading bacterial strain
(1) material is prepared
1, bacterium source and bacterium gather environment
(1) waterworks, Shanghai City, bacterium source biological activated carbon filter;
(2) absolute altitude 0.75 m at the bottom of collection environmental organism activated carbon filter, support bed thickness 0.55 mm, gac bed thickness 1.5 m, charcoal layer top mark is high is 1.30 m, establishes filtering layer depth of the water submerging 1.00 m, design water level 2.30 m in filter tank.Adopt large resistance water distribution system at the bottom of pond, water backwash rate 8 ~ 12 L/m2.s, the filtrate rate of expansion is controlled at 33%.Current gac individual layer filtrate thickness 1500 mm, filtering velocity 9.6 m/h, be 9.4 min duration of contact.
2, humic acids micro-polluted water preparation
Humic acids Shanghai huge maple chemistry Science and Technology Ltd., Ju Feng chemical plant, Jiashan produces.Lot number: 100301; Moisture 5%; Ignition residue 5%; Muriate 0.05%; Vitriol 0.05%.
The humic acids storing solution: take the 1g humic acids, dissolve with 1mol/LNaOH, the tap water dilution is settled to 1000mL, and adjusting pH is 7.0, keeps in Dark Place.
The humic acids micro-polluted water: get 15mL humic acids storing solution, tap water dilutes and is settled to 1000mL.
3, substratum
Enrichment medium: extractum carnis 5g, peptone 10g, sodium-chlor 5g, pH 7.0, distilled water 1000mL.
Domestication substratum I: extractum carnis 0.1g, peptone 0.2g, sodium-chlor 0.1g, humic acids 0.005g, agar 20g, pH 7.0, distilled water 1000mL.
Domestication substratum II: extractum carnis 0.01g, peptone 0.02g, sodium-chlor 0.01g, humic acids 0.010g, agar 20g, pH 7.0, distilled water 1000mL.
Domestication substratum III: humic acids 0.015g, agar 20g, pH 7.0, distilled water 1000mL.
Above substratum is standby after respectively at 121 ℃ of autoclaving 20min.
4, experimental instruments
Constant temperature oscillator;
The illumination bio-incubator;
Steam sterilization pan;
Ultrasonic Cleaners;
Opticmicroscope;
Bechtop;
Portable pH meter;
The circulating water type vacuum pump;
UV-4802 twin-beam ultraviolet-visible pectrophotometer;
PCR reaction amplification instrument (Canadian BBI company);
3730 order-checking row analysers (American AB I company);
SW-CJ-1D clean bench (Jiangsu revive purifying instrument factory);
DK-8D type electric heating constant temperature tank (the gloomy reliable Instrument Ltd. that tests in Shanghai);
DYY-8 type voltage stabilization and current stabilization electrophoresis apparatus (Shanghai Qi Te Analytical Instrument Co., Ltd);
YXJ-2 whizzer (Hunan instrument whizzer instrument company limited);
The miniature electrophoresis chamber of H6-1 (Shanghai lean organic glass products instrument plant);
Gel imaging system (Gene Genius company);
U-3010 ultraviolet-visible spectrophotometer (Hitachi company);
Pipettor (scope 100-1000ml, 20-200ml, 0.5-10ml) (Canadian BBI company);
(2) isolation and screening of humic acid degrading bacterium
1, the separation of bacterium
Get biological activated carbon sample 5 g, be placed in the aseptic Erlenmeyer flask of the 250mL that the 100mL sterilized water is housed, with homogenizer, shake 30 min, the biofilm detachment on biological activated carbon is entered in water, form mixed bacteria liquid.
Above-mentioned mixed bacteria liquid is carried out to the concentration gradient dilution, gets the bacterium liquid of 1mL after dilution and be added on the nutrient agar that 40mL solidified, with spreading rod fast coating evenly, cultivate 2d in 30 ℃, the different bacterium colony of picking growthhabit carries out purifying, and numbering.
2, the preparation of bacteria suspension
By Enrichment of bacteria medium centrifugal collection bacterium, wash bacterium 3 times with physiological saline, adding sterilized water to adjust bacteria suspension optical density value OD600 is 0.6, standby when to be screened.
3, the screening of bacterium
By bacterium is cultivated by the gradient switching of domestication substratum I-III, thereby being tamed, bacterium adapts to the poor nutrient environment that humic acids exists.Using the growing state of bacterium on domestication substratum III as the primary dcreening operation foundation, and concrete grammar is as follows:
The bacterium that separation is obtained is seeded on domestication substratum I, cultivates 1d, and the bacterium grown on picking substratum I is transferred in domestication substratum II, cultivate 3d, bacterium on the picking II is transferred into the III substratum, cultivates 7d, observes the growing state of bacterium on the III substratum, therefrom filter out fast growth, 5 strain bacteriums that bacterial plaque is large, be numbered respectively F1, F2, F3, F4, F5.
Above-mentioned 5 strain bacteriums are made to bacteria suspension, and the volume ratio by 1% adds in the humic acids micro-polluted water, with COD
mn, UV
254for evaluation index, estimate the degradation property of bacterium to humic acids, therefrom filter out the humic acid degradation bacteria with better degradation effect.
(3) evaluation of humic acid degrading bacterium
1, strain morphology is observed
The bacterium colony projection, smooth glossy, be cream-coloured.
By the humic acid degrading bacterium bacteria suspension of enlarged culturing be fixed, after the pre-treatment such as dehydration, lyophilize 48h, obtain powder, after metal spraying processing, amplifies 10000 times and observe ne ar under the JSM-6700F high resolution scanning electron microscope.
2, identify
The humic acid degrading bacterium that screening is obtained carries out 16S rDNA gene sequencing, and the gene order obtained is logged in to NCBI(National Center of Biotechnology Information) database, result show its with
acidovoraxsp. the homology of MN33.2 is 99%.The sequence signature obtained according to form as shown in Figure 3 and evaluation, this bacterium be shaft-like acidovorax facilis (
acidovoraxsp.).
Two, the application of humic acid degrading bacterium
By described humic acid degrading bacterium enlarged culturing, by the centrifugal collection of its enrichment culture liquid bacterium, with physiological saline, wash bacterium 3 times, adding sterilized water to adjust bacteria suspension optical density value OD600 is 0.6, the ratio with 1% is added in the micro-polluted water containing humic acids.
The humic acid degrading bacterium is inoculated in enrichment medium, cultivates 24h for 30 ℃, make bacteria suspension, in the humic acids micro-polluted water that 1% the inoculum size access humic acids concentration of take is 15mg/L, estimate its degradation effect to humic acids.As illustrated in fig. 1 and 2, after the reaction times is 24h, the clearance of the micro-polluted water COD that it is 15mg/L to the humic acids starting point concentration is 33.8%, UV
254clearance be 48.1%.
Three, the influence factor of humic acid degrading bacterium degradation property
Get humic acids starting point concentration, pH and temperature as the principal element that affects humic acid degrading bacterium degradation effect, take COD as index, estimate the impact of these factors.
1, humic acids starting point concentration
Humic acid degrading bacterium bacteria suspension is added in the humic acids micro-polluted water of different concns with 1.0% ratio, and humic acids concentration is respectively 5mg/L, 10mg/L, 15mg/L, 20mg/L, and water sample pH is 7.0, in 20 ℃ of reaction 24h and 48h, measures COD.
2、pH
Water sample pH is set and is respectively 3.0,4.0,5.0,6.0,7.0,8.0, humic acid degrading bacterium bacteria suspension be take to 1.0% ratio and be added in the humic acids micro-polluted water that humic acids concentration is 15mg/L, in 20 ℃ of reaction 24h and 48h, measure COD.
3, temperature
Temperature of reaction is set and is respectively 20 ℃, 25 ℃, 30 ℃, 35 ℃, 40 ℃, by humic acid degrading bacterium bacteria suspension take 1.0% ratio be added to pH as 7.0 and the humic acids concentration humic acids micro-polluted water that is 15mg/L in, reaction 24h and 48h, measure COD.
Research humic acids starting point concentration, pH, these three factors of temperature affect result as shown in Fig. 4-6 to humic acid degrading bacterium degradation property, and when the humic acids starting point concentration is 20mg/L, when temperature of reaction is 35 ℃, this bacterium more can be brought into play its degradation property to humic acids; Micro-polluted water is the growth that acidity is conducive to this acidovorax facilis, promotes its degraded to humic acids.
The bacterial strain that the present invention separates, filters out, be a strain acidovorax facilis (
acidovoraxsp.), it has the function of degraded humic acids.This can widen people to acidovorax facilis in its function aspects applied research thinking, and, for the degraded humic acids provides bacterium source and the technology of use, there is stronger actual application value.
Claims (4)
1. a humic acid degrading bacterial strain, is characterized in that this bacterial strain is to separate acquisition from the biological activated carbon of waterworks, Shanghai City biological activated carbon filter; This bacterial strain be a strain acidovorax facilis (
acidovoraxsp.), the bacterial strain preserving number is: CGMCC No.6564, on September 20th, 2012 is in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms.
2. a kind of humic acid degradation bacteria strains as claimed in claim 1, is characterized in that this bacterial strain is on the domestication substratum, cultivates 7 days under 30 ℃, and the bacterium colony projection, smooth glossy, be cream-coloured; It is shaft-like observing its form under scanning electronic microscope.
3. the screening method of a humic acid degrading bacterial strain is characterized in that comprising the following steps:
Isolate the different bacterium of growthhabit from the biological activated carbon collected;
The bacterium that separation is obtained carries out gradient by the domestication substratum I-III containing the different concns humic acids and cultivates domestication;
Domestication substratum I: extractum carnis 0.1g, peptone 0.2g, sodium-chlor 0.1g, humic acids 0.005g, agar 20g, pH 7.0, distilled water 1000mL; Domestication substratum II: extractum carnis 0.01g, peptone 0.02g, sodium-chlor 0.01g, humic acids 0.010g, agar 20g, pH 7.0, distilled water 1000mL; Domestication substratum III: humic acids 0.015g, agar 20g, pH 7.0, distilled water 1000mL; The bacterium that separation is obtained is cultivated by the gradient switching of domestication substratum I-III, makes bacterium be tamed and then adapt to the poor nutrient environment of humic acids existence; Using the growing state of bacterium on domestication substratum III as the primary dcreening operation foundation, filter out 5 strain fast growths, bacterium that bacterial plaque is large, number consecutively is F1, F2, F3, F4, F5;
Above-mentioned 5 kinds of bacterial strains are added in the humic acids micro-polluted water, with COD
mn, UV
254for evaluation index, estimate the degradation property of bacterium to humic acids, further filter out the humic acid degrading bacterium with better degradation effect.
4. the application method of a humic acid degrading bacterial strain, is characterized in that described bacterial strain is applied in the processing containing the micro-polluted water of humic acids; Treatment process is: this bacterium colony of picking, make bacteria suspension after enlarged culturing, and regulating OD600 is 0.6, is that 1% ratio is inoculated in the humic acids micro-polluted water by volume, measures COD, UV
254; The clearance of the micro-polluted water COD that humic acid degrading bacterium in the present invention is 15mg/L to the humic acids starting point concentration is 33.8%, UV
254clearance be 48.1%; Humic acid degrading bacterium in the present invention is 20mg/L at the humic acids starting point concentration, when temperature of reaction is 35 ℃, and the humic acids of degrading better when micro-polluted water is acidity.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923852A (en) * | 2014-03-07 | 2014-07-16 | 江苏农林职业技术学院 | Acidovorax sp. and application |
| CN104819951A (en) * | 2015-05-09 | 2015-08-05 | 西北农林科技大学 | Soil degrading bacteria and experimental method of combinations of soil degrading bacteria for degrading humic acid (HA) in farmland |
| CN112812970A (en) * | 2021-03-19 | 2021-05-18 | 上饶师范学院 | Method for reinforcing utilization of humic acid in pig farm biogas slurry by microalgae through nano titanium dioxide |
| CN115011514A (en) * | 2022-06-10 | 2022-09-06 | 兰州理工大学 | Humic acid degrading bacteria and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021126A (en) * | 2010-05-18 | 2011-04-20 | 中国石油天然气集团公司 | Salt-resistant degrading bacterium for treating high-concentration persistent organic wastewater and application thereof |
-
2012
- 2012-11-07 CN CN201210440327.1A patent/CN103468598B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021126A (en) * | 2010-05-18 | 2011-04-20 | 中国石油天然气集团公司 | Salt-resistant degrading bacterium for treating high-concentration persistent organic wastewater and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 刘志培 等: "食酸丛毛单胞菌AN3菌株降解苯胺的研究", 《应用与环境生物学报》 * |
| 周洁 等: "食酸戴尔福特菌USTB04生物降解微囊藻毒素的活性研究", 《科学技术与工程》 * |
| 徐子晴 等: "PHBV降解菌株的选育及其降解性能研究", 《塑料科技》 * |
| 韩云平 等: "¹ 腐殖酸高效降解菌的筛选研究", 《山西大学学报(自然科学版)》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103923852A (en) * | 2014-03-07 | 2014-07-16 | 江苏农林职业技术学院 | Acidovorax sp. and application |
| CN103923852B (en) * | 2014-03-07 | 2016-01-13 | 江苏农林职业技术学院 | A kind of acidovorax facilis and application thereof |
| CN104819951A (en) * | 2015-05-09 | 2015-08-05 | 西北农林科技大学 | Soil degrading bacteria and experimental method of combinations of soil degrading bacteria for degrading humic acid (HA) in farmland |
| CN112812970A (en) * | 2021-03-19 | 2021-05-18 | 上饶师范学院 | Method for reinforcing utilization of humic acid in pig farm biogas slurry by microalgae through nano titanium dioxide |
| CN115011514A (en) * | 2022-06-10 | 2022-09-06 | 兰州理工大学 | Humic acid degrading bacteria and application thereof |
| CN115011514B (en) * | 2022-06-10 | 2023-11-10 | 兰州理工大学 | Humic acid degrading bacteria and application thereof |
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