CN115011504B - Bacillus bailii XY40-1 and application thereof - Google Patents
Bacillus bailii XY40-1 and application thereof Download PDFInfo
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- CN115011504B CN115011504B CN202210422749.XA CN202210422749A CN115011504B CN 115011504 B CN115011504 B CN 115011504B CN 202210422749 A CN202210422749 A CN 202210422749A CN 115011504 B CN115011504 B CN 115011504B
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- velezensis
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- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 51
- 241000223208 Curvularia Species 0.000 claims abstract description 15
- 241000159512 Geotrichum Species 0.000 claims abstract description 13
- 244000060011 Cocos nucifera Species 0.000 claims abstract description 6
- 235000013162 Cocos nucifera Nutrition 0.000 claims abstract description 6
- 230000000813 microbial effect Effects 0.000 claims description 12
- 230000002401 inhibitory effect Effects 0.000 claims description 7
- 230000012010 growth Effects 0.000 claims description 5
- 241000223600 Alternaria Species 0.000 claims description 4
- 241000233616 Phytophthora capsici Species 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 244000000003 plant pathogen Species 0.000 claims 2
- 235000002566 Capsicum Nutrition 0.000 abstract description 6
- 238000004321 preservation Methods 0.000 abstract description 5
- 239000006002 Pepper Substances 0.000 abstract description 4
- 235000016761 Piper aduncum Nutrition 0.000 abstract description 4
- 235000017804 Piper guineense Nutrition 0.000 abstract description 4
- 235000008184 Piper nigrum Nutrition 0.000 abstract description 4
- 230000008485 antagonism Effects 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 3
- 241001503951 Phoma Species 0.000 abstract description 2
- 241000722363 Piper Species 0.000 abstract 2
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 30
- 241000196324 Embryophyta Species 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 241000233614 Phytophthora Species 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 230000003042 antagnostic effect Effects 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 240000008574 Capsicum frutescens Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000192132 Leuconostoc Species 0.000 description 2
- 244000203593 Piper nigrum Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000443 biocontrol Effects 0.000 description 2
- 239000001390 capsicum minimum Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000233622 Phytophthora infestans Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229940071160 cocoate Drugs 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
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- Plant Pathology (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
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Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to bacillus beijerinckii XY40-1 and application thereof. The bacillus belicus XY40-1 is preserved in China Center for Type Culture Collection (CCTCC) at the 3 rd month 29 of 2022, and is isolated from pepper leaves of Hunan Shake-ground pepper base with the preservation number of M2022342. The bacillus bailii XY40-1 has better antagonism on geotrichum citri, phoma cocoanut and Curvularia zeae.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to bacillus beijerinckii (Bacillus velezensis) XY40-1 and application thereof.
Background
Plant diseases are always an important factor threatening agricultural production, and a large amount of chemical pesticides are used for a long time, so that pathogenic bacteria generate drug resistance, and pesticide residues are harmful to people, livestock and environment. Biological control is a method for inhibiting or eliminating harmful organisms by utilizing beneficial organisms or other organisms, has the advantages of no toxicity, no harm, no pollution, difficult generation of drug resistance and the like, and therefore, the biological control is possible to replace chemical agents to become an environment-friendly and safe choice in the future.
Endophytic bacteria are isolated from surface sterilized plant tissue or obtained from the interior of plants, but their presence does not alter the phenotypic characteristics and function of the plant. Endophytes are prevalent in higher plants, are diverse in different species of plant individuals and different locations, and generally, endophytes are more abundant in leaf locations than in other locations, and also grow with the growth of the leaves. Endophytic bacteria have stable living space in plants, are not easily influenced by environmental conditions, have inhibiting effect on certain pathogenic bacteria, and have growth promoting effect on plants. Therefore, endophytes are utilized as potential microbial pesticides, yield increasing bacteria or as potential biocontrol carrier bacteria in biocontrol.
Chinese patent (CN 112899196A) discloses the application of Bacillus belicus in preventing and treating clubroot of cruciferae; chinese patent (CN 110699275A) discloses that Bacillus bailii antagonizes gray mold primordium of picked fruits and vegetables; chinese patent (CN 111534460A) discloses Bacillus belicus highly effective against Fusarium graminearum.
However, it has not been studied in the prior art whether Bacillus belicus is antagonistic to Geotrichum citricum, leuconostoc cocoanut and Curvularia zeae.
Disclosure of Invention
In order to solve the technical problems, the invention provides bacillus beijerinus (Bacillus velezensis) XY40-1 and application thereof.
Bacillus bailii (Bacillus velezensis) XY40-1, wherein the Bacillus bailii (Bacillus velezensis) XY40-1 is preserved in China Center for Type Culture Collection (CCTCC) No. M2022342 at 2022 and 3 month 29.
A microbial agent comprising bacillus bailii (Bacillus velezensis) XY40-1.
Preferably, the microbial agent is a liquid microbial agent or a solid microbial agent.
Use of bacillus bailii (Bacillus velezensis) XY40-1 or a microbial agent comprising bacillus bailii (Bacillus velezensis) XY40-1 for inhibiting growth of plant pathogenic bacteria.
Preferably, the phytopathogenic bacteria include any one of geotrichum citri, curvularia zeae and Curvularia cocoate.
The invention has the beneficial effects that:
the invention provides the bacillus belicus (Bacillus velezensis) XY40-1, and discovers that the bacillus belicus XY40-1 has good antagonism on geotrichum citri, alternaria coconut and Curvularia zeae, and finds a new direction for preventing and controlling plant diseases.
Drawings
FIG. 1 is a photograph of Bacillus belicus (Bacillus velezensis) observed under an XY40-1 microscope (1000X).
FIG. 2 shows the morphology of colonies on the front side of Bacillus belicus (Bacillus velezensis) XY40-1 plates.
FIG. 3 is a tree analysis of Bacillus beleiensis (Bacillus velezensis) XY40-1.
FIG. 4 is a graph showing the antagonistic effect of Bacillus belicus (Bacillus velezensis) XY40-1 on phytophthora (Phytophthora capsici), wherein A: phytophthora infestans; b: the culture effect of Bacillus bailii XY40-1 and phytophthora on is shown.
FIG. 5 is a graph showing the antagonistic effect of Bacillus belicus (Bacillus velezensis) XY40-1 against pathogenic bacteria, wherein A: curvularia zeae; b: graph of the culture effect of Bacillus belicus XY40-1 on Curvularia zeae on .
FIG. 6 is a graph showing the antagonistic effect of Bacillus belicus (Bacillus velezensis) XY40-1 against pathogenic bacteria, wherein A: the fungus Cocois griseofulva; b: the culture effect diagram of Bacillus belicus XY40-1 and Cocois griseofulva on is shown; c, geotrichum citri; and D, culturing effect graph of bacillus bailii XY40-1 and Geotrichum citri on .
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides bacillus bailii (Bacillus velezensis) XY40-1, which is separated from pepper leaves of Hunan Shake ground pepper base, wherein the bacillus bailii (Bacillus velezensis) XY40-1 is preserved in the China center for type culture Collection, with a preservation address of China university of Wuhan and Wuhan, and a preservation number of CCTCC NO: M2022342 at 2022 and 3 months 29. Other microorganisms are known in the art.
The strain medium used in the following examples is as follows:
LB medium (g/L): 10g/L of tryptone, 5g/L of yeast extract, 10g/L of sodium chloride and 15g/L of agar powder, adding water for dissolution, finally fixing the volume to 1000mL, and sterilizing under high pressure (121 ℃ C., 30 min) with pH of 7.0+/-0.2.
PDA solid medium (g/L): 6g of potato soaked powder, 20g of glucose and 20g of agar, adding water for dissolving, finally fixing the volume to 1000mL, and sterilizing under high pressure (121 ℃ C., 30 min) with pH of 5.6+/-0.2.
EXAMPLE 1 isolation, identification, purification and preservation of Bacillus bailii (Bacillus velezensis) XY40-1 Strain
(1) Isolation and identification
The separation and identification method of bacillus bailii (Bacillus velezensis) XY40-1 comprises the following steps: taking capsicum leaves of a capsicum base ground by longsand Hunan, respectively adopting a tissue culture method and a dilution coating flat plate method to separate endophytic bacteria of the leaves of capsicum plants after the surface sterilization, screening to obtain a strain capable of inhibiting phytophthora capsici growth through a flat plate counter experiment, and observing a photo by a bacillus beijerinckii (Bacillus velezensis) XY40-1 microscope (1000X); FIG. 2 shows the morphology of colonies on the front side of Bacillus belicus (Bacillus velezensis) XY40-1 plates. Carrying out streak purification on the strain on an LB plate; the bacterial universal primer (universal primer 27F 5'-AGAGTTTGATCCTGGCTCAG-3' (shown in SEQ ID NO. 1) and 14952R 5'-TACGGCTACCTTGTTACGACTT-3' (shown in SEQ ID NO. 2)) are utilized to carry out 16SrRNA gene amplification, and the gene sequence (the nucleotide sequence of the 16SrRNA gene is shown in SEQ ID NO. 3) obtained after the PCR product is sent to sequence is compared and analyzed in NCBI, so that the classification status of the strain is determined. Sequencing results BLAST alignment was performed on NCBI website to determine the taxonomic status of the closely related bacterial strains. The comparison result shows that: the strain has 99.79% affinity with Bacillus belicus (Bacillus velezensis), designated Bacillus belicus (Bacillus velezensis) XY40-1. The phylogenetic tree of Bacillus belicus (Bacillus velezensis) XY40-1 was constructed using MEGA6.0, and the results are shown in FIG. 3;
(2) Purification and preservation of strains
The flat plate scribing method is adopted: and (3) picking out a colony capable of inhibiting phytophthora growth, scribing on a new LB plate, placing the new LB plate in a biochemical incubator at 28 ℃, culturing the new LB plate in an inverted mode for more than 24 hours, and picking out a single colony, wherein the number of the single colony is XY40-1. Inoculating Bacillus bailii (Bacillus velezensis) XY40-1 strain XY40-1 into LB liquid medium, culturing at 28deg.C in shaking table at 200rpm for 24 hr, sucking 1mL fermentation broth of Bacillus bailii (Bacillus velezensis) XY40-1 strain and 1mL sterile glycerol of 50%, mixing with gentle shaking, and standing at-80deg.C for long term storage.
Example 2 test of Bacillus bailii (Bacillus velezensis) XY40-1 antagonism against Phytophthora, geotrichum citri, curvularia coco and Curvularia zeae
The plate counter method is adopted: spot-grafting phytophthora, geotrichum citri, coco gray spot bacteria and corn curvularia bacteria on the center of the PDA flat plate respectively, and spot-grafting strain bacillus bailii (Bacillus velezensis) XY40-1 on the position 2cm away from the center of the culture medium by a crisscross method; the control group is not inoculated with the strain Bacillus bailii (Bacillus velezensis) XY40-1, and is cultured at constant temperature of 28 ℃ for 5-7 days, after the control phytophthora, geotrichum citricum, alternaria coco and Curvularia zeae grow on the flat plate, the width of the antibacterial zone is measured, and each group is treated for 3 times.
Antibacterial ratio (%) = (control colony diameter-treated colony diameter)/control colony diameter ×100%
TABLE 1 inhibitory Effect of Bacillus bailii XY40-1 on Phytophthora capsici
TABLE 2 inhibition of Bacillus bailii XY40-1 against Geotrichum citrulli, leuconostoc cocoanut, curvularia zeae
The antagonistic effect of Bacillus belicus (Bacillus velezensis) XY40-1 on phytophthora, geotrichum citri, alternaria coco, curvularia zeae is shown in FIG. 4-6 and Table 1-2; by a plate pair culture method, the bacillus beijerinus XY40-1 has good antagonism on geotrichum citri, phoma cocoanut and Curvularia zeae.
Sequence listing
<110> Hunan province vegetable institute
<120> Bacillus bailii XY40-1 strain and application thereof
<141> 2022-04-21
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213> Artificial sequence (Artificial sequence)
<400> 1
agagtttgat cctggctcag 20
<210> 2
<211> 22
<212> DNA
<213> Artificial sequence (Artificial sequence)
<400> 2
tacggctacc ttgttacgac tt 22
<210> 3
<211> 1449
<212> DNA
<213> Bacillus velezensis
<400> 3
ggagcttgct ccctgatgtt agcggcggac gggtgagtaa cacgtgggta acctgcctgt 60
aagactggga taactccggg aaaccggggc taataccgga tggttgtctg aaccgcatgg 120
ttcagacata aaaggtggct tcggctacca cttacagatg gacccgcggc gcattagcta 180
gttggtgagg taacggctca ccaaggcgac gatgcgtagc cgacctgaga gggtgatcgg 240
ccacactggg actgagacac ggcccagact cctacgggag gcagcagtag ggaatcttcc 300
gcaatggacg aaagtctgac ggagcaacgc cgcgtgagtg atgaaggttt tcggatcgta 360
aagctctgtt gttagggaag aacaagtgcc gttcaaatag ggcggcacct tgacggtacc 420
taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 480
gttgtccgga attattgggc gtaaagggct cgcaggcggt ttcttaagtc tgatgtgaaa 540
gcccccggct caaccgggga gggtcattgg aaactgggga acttgagtgc agaagaggag 600
agtggaattc cacgtgtagc ggtgaaatgc gtagagatgt ggaggaacac cagtggcgaa 660
ggcgactctc tggtctgtaa ctgacgctga ggagcgaaag cgtggggagc gaacaggatt 720
agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttagggg gtttccgccc 780
cttagtgctg cagctaacgc attaagcact ccgcctgggg agtacggtcg caagactgaa 840
actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca 900
acgcgaagaa ccttaccagg tcttgacatc ctctgacaat cctagagata ggacgtcccc 960
ttcgggggca gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1020
gttaagtccc gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc 1080
taaggtgact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1140
cttatgacct gggctacaca cgtgctacaa tggacagaac aaagggcagc gaaaccgcga 1200
ggttaagcca atcccacaaa tctgttctca gttcggatcg cagtctgcaa ctcgactgcg 1260
tgaagctgga atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc 1320
ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc 1380
tttatggagc cagccgccga aggtgggaca gatgattggg gtgaagtcgt aacaaggtag 1440
ccgtatcgg 1449
Claims (4)
1. Bacillus bailii strainBacillus velezensis) XY40-1, characterized in that the Bacillus bailii isBacillus velezensis) XY40-1 was preserved in China Center for Type Culture Collection (CCTCC) No. M2022342 at 2022, 3 and 29.
2. A microbial agent comprising the Bacillus bailii of claim 1Bacillus velezensis）XY40－1。
3. The microbial agent of claim 2, wherein the microbial agent is a liquid microbial agent or a solid microbial agent.
4. The bacillus belicus strain according to claim 1Bacillus velezensis) Use of XY40-1 or a microbial agent according to any one of claims 2-3 for inhibiting the growth of a plant pathogen, said plant pathogen being any one of geotrichum citricola, curvularia zeae, alternaria cocoanut and phytophthora capsici.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210422749.XA CN115011504B (en) | 2022-04-21 | 2022-04-21 | Bacillus bailii XY40-1 and application thereof |
| US18/163,866 US20230337682A1 (en) | 2022-04-21 | 2023-02-02 | Bacillus velezensis, fermentation method, fermentation product and its application |
| AU2023203456A AU2023203456B1 (en) | 2022-04-21 | 2023-06-02 | Bacillus Velezensis, Fermentation Method, Fermentation Product and its Application |
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| CN202210422749.XA CN115011504B (en) | 2022-04-21 | 2022-04-21 | Bacillus bailii XY40-1 and application thereof |
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| CN115011504A CN115011504A (en) | 2022-09-06 |
| CN115011504B true CN115011504B (en) | 2023-08-11 |
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| AU (1) | AU2023203456B1 (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108265012A (en) * | 2016-12-30 | 2018-07-10 | 北京绿色农华作物科技有限公司 | A kind of Bei Laisi Bacillus strains and its microbial inoculum and application |
| CN109055281A (en) * | 2018-09-19 | 2018-12-21 | 北京化工大学 | Bei Laisi bacillus ZF2 and its application in control of plant disease |
| CN109370939A (en) * | 2018-11-06 | 2019-02-22 | 上海交通大学 | One plant of Bei Laisi bacillus and its separation method and application |
| CN110283742A (en) * | 2019-06-17 | 2019-09-27 | 北京农业生物技术研究中心 | The Bei Laisi bacillus of one plant of broad-spectrum disease resistance and its application |
| KR102078252B1 (en) * | 2018-12-18 | 2020-02-17 | 경기도 | Bacillus velezensis ARRI17 strain having antifungal activity against Root Rot Pathogen of ginseng and Use Thereof |
| CN113528370A (en) * | 2021-04-22 | 2021-10-22 | 上海大学 | Bacillus belgii strain and application thereof |
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| US20180020676A1 (en) * | 2014-12-29 | 2018-01-25 | Fmc Corporation | Bacillus velezensis rti301 compositions and methods of use for benefiting plant growth and treating plant disease |
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| CN108265012A (en) * | 2016-12-30 | 2018-07-10 | 北京绿色农华作物科技有限公司 | A kind of Bei Laisi Bacillus strains and its microbial inoculum and application |
| CN109055281A (en) * | 2018-09-19 | 2018-12-21 | 北京化工大学 | Bei Laisi bacillus ZF2 and its application in control of plant disease |
| CN109370939A (en) * | 2018-11-06 | 2019-02-22 | 上海交通大学 | One plant of Bei Laisi bacillus and its separation method and application |
| KR102078252B1 (en) * | 2018-12-18 | 2020-02-17 | 경기도 | Bacillus velezensis ARRI17 strain having antifungal activity against Root Rot Pathogen of ginseng and Use Thereof |
| CN110283742A (en) * | 2019-06-17 | 2019-09-27 | 北京农业生物技术研究中心 | The Bei Laisi bacillus of one plant of broad-spectrum disease resistance and its application |
| CN113528370A (en) * | 2021-04-22 | 2021-10-22 | 上海大学 | Bacillus belgii strain and application thereof |
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| 新型生防微生物因子贝莱斯芽孢杆菌 （Bacillus velezensis）的研究与应用;陶永梅等;中国植保导刊;第39卷(第9期);26-33 * |
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| CN115011504A (en) | 2022-09-06 |
| US20230337682A1 (en) | 2023-10-26 |
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