CN115006602A - Bone repair material with high calcium and phosphorus content and application thereof - Google Patents
Bone repair material with high calcium and phosphorus content and application thereof Download PDFInfo
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- CN115006602A CN115006602A CN202210657808.1A CN202210657808A CN115006602A CN 115006602 A CN115006602 A CN 115006602A CN 202210657808 A CN202210657808 A CN 202210657808A CN 115006602 A CN115006602 A CN 115006602A
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- bone repair
- bioactive glass
- calcium
- polyvinyl alcohol
- sodium alginate
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 41
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Abstract
The invention discloses a bone repair material with high calcium and phosphorus content, which comprises bioactive glass powder, sodium alginate and polyvinyl alcohol, wherein the mass ratio of the bioactive glass powder to the sodium alginate to the polyvinyl alcohol in a mixed solution is (1-10): (2-8): (8-16); wherein, the molar ratio of the calcium content in the bioactive glass powder is 10-36%; the phosphorus content molar ratio is 4-10%, and the bone repair material with high calcium and phosphorus content is applied to preparing a bone repair scaffold, so that the porous repair scaffold with good biological activity and bone induction capability is obtained, and the natural bone structure and components are simulated to promote the repair and regeneration of bone tissues.
Description
Technical Field
The invention relates to the technical field of bone repair, in particular to a bone repair material with high calcium and phosphorus content and a preparation method and application thereof.
Background
With the continuous development of economic society, bone defect caused by traffic accidents, accidental injuries and orthopedic diseases is more and more common, the bone defect seriously affects the living standard and health condition of people, great worry is brought to national economic development and social stability, and the bone defect is a problem to be solved urgently. At present, the traditional autologous bone transplantation and the traditional allogeneic bone transplantation are generally used for clinically treating patients with bone defects, but the autologous bone transplantation has the defects of limited sources, insufficient donors, immunological rejection, disease infection and the like, and cannot meet the requirements of patients on bone repair, so that the clinical demand on bone tissue engineering scaffolds with repair functions is more and more strong, the rapid development of the research on the bone repair scaffolds is promoted, and the research and development of bone repair materials become international research hotspots.
The bone tissue is mainly composed of inorganic substances and organic substances. From the thinking of bionics, the composition and the structure of natural bone tissues are simulated, which is one of the development directions in the field of bone repair. Natural bone tissue has a rich porous structure, and its porous structure is conducive to bone growth or repair processes. The preparation of the bone tissue repair scaffold not only provides three-dimensional space and metabolic environment for cell proliferation, but also determines the shape and size of the new tissue.
Therefore, how to provide a scaffold material with a porous structure, which provides a mild and stable environment for the survival of cells inside the scaffold, and the realization of bone tissue regeneration is a problem that needs to be solved by those skilled in the art.
Disclosure of Invention
In view of the above, the invention provides a bone repair material with high calcium and phosphorus content and application thereof, and obtains a porous repair scaffold with good bioactivity and bone induction capability, which simulates the structure and components of natural bone and promotes the repair and regeneration of bone tissues.
In order to achieve the purpose, the invention adopts the following technical scheme:
a bone repair material with high calcium and phosphorus content comprises bioactive glass powder, sodium alginate and polyvinyl alcohol, wherein the mass ratio of the bioactive glass powder to the sodium alginate to the polyvinyl alcohol is (1-10): (2-8): (8-16); wherein, the molar ratio of the calcium content in the bioactive glass powder is 10-36%; the content of the phosphorus is 4 to 10 percent by mole.
The bioactive glass has obvious advantages in the aspects of bone guidance and bone inductivity as an inorganic material with good functions, and has good development prospect in the field of bone repair scaffolds. Polyvinyl alcohol (PVA) is a degradable water-soluble biopolymer, and its good biocompatibility and processability make it widely used in the field of biomedical engineering. Sodium Alginate (SA) as a natural polysaccharide, its good biocompatibility and its unique gelling properties (Na on G units) + Ion exchange reaction with divalent cations, and stacking of G units to form a cross-linked network structure, thereby forming hydrogel) is an advantage of its application in the biomedical field. The polyvinyl alcohol/sodium alginate hydrogel composite scaffold of the bioactive glass with high calcium and phosphorus content has the components similar to those of natural bone tissues, has good degradation rate, ion release, biocompatibility and osteogenesis induction effect, and meets the requirements of a bone repair process to a certain extent.
The invention also discloses application of the bone repair material with high calcium and phosphorus content in preparing a bone repair bracket as the same inventive concept as the technical scheme.
In the polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold containing bioactive glass with high calcium and phosphorus content, the biological compatibility of the material per se makes the material become an excellent choice for a bone tissue repair scaffold, and simultaneously the system utilizes the characteristics and chemical action of the material per se to enable the polyvinyl alcohol/sodium alginate system to generate gel reaction, so that the overall stability and mechanical property of the scaffold are improved, and meanwhile, water forms ice crystals due to low temperature in the pre-freezing forming process, and the ice crystals are sublimated to form holes during freeze drying, so that a porous structure similar to natural bones is constructed, thereby promoting cell adhesion and ingrowth and improving the bone repair effect.
The invention also provides a bone repair scaffold with high calcium and phosphorus content, which is characterized by being prepared from the bone repair material.
The invention also provides a preparation method of the bone repair scaffold with high calcium and phosphorus content, which is the same as the technical proposal, and comprises the following steps:
(1) adding bioactive glass powder into deionized water, and performing ultrasonic and magnetic stirring to uniformly disperse the bioactive glass powder to obtain a suspension A;
(2) adding polyvinyl alcohol into the suspension A, and magnetically stirring in a water bath to uniformly disperse the polyvinyl alcohol to obtain a suspension B;
(3) adding sodium alginate into the suspension B, and magnetically stirring in a water bath to uniformly mix the sodium alginate and the suspension B to obtain a mixed solution C;
(4) and (3) carrying out constant-temperature vacuum treatment on the mixed solution C, pouring the treated mixed solution C into a polytetrafluoroethylene mold plate, pre-freezing and forming at the temperature of minus 20 ℃, and then carrying out freeze drying to obtain the porous bioactive glass polyvinyl alcohol/sodium alginate hydrogel composite bone scaffold with high calcium and phosphorus content.
The invention adopts a method of combining self-crosslinking and phase separation technologies of a calcium ion-promoted gel system rich in bioactive glass to prepare the polyvinyl alcohol/sodium alginate hydrogel composite scaffold containing bioactive glass with high calcium and phosphorus contents. The composite scaffold has good mechanical property, and Si, Ca and P ions released by the bioactive glass promote the gelling of an organic system and simultaneously promote the rapid proceeding of a bone repair process; meanwhile, the porous structure formed in the freeze drying process of the scaffold is also beneficial to body fluid infiltration, nutrient substance transportation and cell growth. The scaffold is simple in preparation method, convenient and fast to operate, and has wide application prospect in the field of bone tissue repair.
As a preferable technical scheme of the technical scheme, in the step (1), the concentration of the bioactive glass powder in the suspension A is 1-10 w/v%.
As a preferable embodiment of the above-mentioned means, in the step (2), the concentration of the polyvinyl alcohol in the suspension B is 8 to 16 w/v%.
As a preferable embodiment of the above-mentioned means, the temperature of the water bath in the step (2) and the step (3) is 75 to 85 DEG C
As a preferable technical scheme of the technical scheme, the concentration of the sodium alginate in the mixed solution C in the step (3) is 2-8 w/v%.
As a preferable technical scheme of the technical scheme, the constant-temperature vacuum treatment condition in the step (4) is 80 ℃, and the vacuum treatment is carried out for 30 min.
According to the technical scheme, the technical effects of the invention are as follows:
(1) the invention selects the bioactive glass with high calcium and phosphorus content and the polyvinyl alcohol/sodium alginate hydrogel to compound, so as to obtain the composite scaffold with self-crosslinking capability, the mechanical property of the system is improved in a physical layer due to the addition of the bioactive glass, and meanwhile, calcium ions and the hydrogel system are crosslinked due to the high calcium content in the bioactive glass, so that the property of the hydrogel which is easy to hydrolyze is improved in a chemical layer.
(2) The selected specific bioactive glass with high calcium and phosphorus content can not only react with hydrogel to generate crosslinking to balance the degradation rate of the hydrogel, but also be beneficial to slow release of inorganic ions, and the system is beneficial to stable cell growth and metabolic process.
(3) The polyvinyl alcohol/sodium alginate hydrogel composite scaffold containing the bioactive glass with high calcium and phosphorus content can be applied to the fields of bone tissue repair, bone tissue engineering and the like.
(4) The mixed solution C obtained by adopting the steps provides favorable conditions for obtaining a porous structure similar to natural bones by using a separation technology.
(5) The porous structure obtained by the separation technology is similar to that of natural bones, and is beneficial to body fluid infiltration, nutrient substance transportation and cell ingrowth, so that the rapid progress of bone repair is promoted.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a 400-fold enlarged tungsten filament scanning electron microscope image of the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel porous composite support with high content of calcium and phosphorus;
FIG. 2 is a scanning electron microscope image of a tungsten filament of 800 times of the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel porous composite bracket with high content of calcium and phosphorus;
FIG. 3 is a drawing showing the co-culture of the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold with high content of calcium and phosphorus and the mouse bone marrow mesenchymal stem cells for 4 days of live-dead staining.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
A bone repair material with high calcium and phosphorus content comprises bioactive glass powder, sodium alginate and polyvinyl alcohol, wherein the mass ratio of the bioactive glass powder to the sodium alginate to the polyvinyl alcohol is (1-10): (2-8): (8-16); wherein, the molar ratio of the calcium content in the bioactive glass powder is 10-36%; the mol ratio of the phosphorus content is 4-10%.
The bone repair material in the embodiment 1 is applied to the preparation of a bone repair bracket, and the process is shown in the embodiment 2 to the embodiment 4;
example 2
A polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold containing bioactive glass with high calcium and phosphorus content is prepared by the following steps:
adding 0.1g of bioactive glass into 10mL of deionized water, ultrasonically dispersing for 15min, and magnetically stirring for 15min to uniformly disperse the bioactive glass to obtain a suspension A; adding 0.8g of polyvinyl alcohol into the obtained suspension A, and magnetically stirring for 1h in a water bath at 95 ℃ to uniformly disperse the polyvinyl alcohol in the suspension to obtain a suspension B; adding 0.2g of sodium alginate into the obtained suspension B in a water bath at 75 ℃, and magnetically stirring for 30min to completely dissolve the sodium alginate to obtain a mixed solution C; covering the preservative film with the mixed solution C, pricking holes, placing the mixture into a vacuum drying oven at 80 ℃ for constant temperature for 30min, then pouring the mixture into a polytetrafluoroethylene mold, pre-freezing the mixture at-20 ℃, and then freeze-drying the mixture to obtain the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel composite scaffold with a porous structure and high calcium and phosphorus content.
Example 3
A polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold containing bioactive glass with high calcium and phosphorus content is prepared by the following steps:
adding 0.5g of bioactive glass into 10mL of deionized water, performing ultrasonic dispersion for 15min, and performing magnetic stirring for 15min to uniformly disperse the bioactive glass to obtain a suspension A; adding 1.6g of polyvinyl alcohol into the obtained suspension A, and magnetically stirring for 1h in a water bath at 95 ℃ to uniformly disperse the polyvinyl alcohol in the suspension to obtain a suspension B; adding 0.4g of sodium alginate into the obtained suspension B in a water bath at the temperature of 80 ℃, and magnetically stirring for 30min to completely dissolve the sodium alginate to obtain a mixed solution C; covering the preservative film with the mixed solution C, pricking holes, placing the mixture into a vacuum drying oven at 80 ℃ for constant temperature for 30min, then pouring the mixture into a polytetrafluoroethylene mold, pre-freezing the mixture at-20 ℃, and then freeze-drying the mixture to obtain the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel composite scaffold with a porous structure and high calcium and phosphorus content.
Example 4
A polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold containing bioactive glass with high calcium and phosphorus content is prepared by the following steps:
adding 0.2g of bioactive glass into 10mL of deionized water, ultrasonically dispersing for 15min, and magnetically stirring for 15min to uniformly disperse the bioactive glass to obtain a suspension A; adding 1.2g of polyvinyl alcohol into the obtained suspension A, and magnetically stirring for 1h in a water bath at 95 ℃ to uniformly disperse the polyvinyl alcohol in the suspension to obtain a suspension B; adding 0.8g of sodium alginate into the obtained suspension B in a water bath at 85 ℃, and magnetically stirring for 30min to completely dissolve the sodium alginate to obtain a mixed solution C; covering the preservative film with the mixed solution C, pricking holes, placing the mixture into a vacuum drying oven at 80 ℃ for constant temperature for 30min, then pouring the mixture into a polytetrafluoroethylene mold, pre-freezing the mixture at-20 ℃, and then freeze-drying the mixture to obtain the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel composite scaffold with a porous structure and high calcium and phosphorus content.
Scanning electron micrographs of the bioactive glass/polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold with high calcium and phosphorus content obtained in example 3 are shown in fig. 1 and fig. 2, and a live-dead staining pattern of the porous composite scaffold and mouse bone marrow mesenchymal stem cells co-cultured for 4 days is shown in fig. 3. As can be seen from the figures 1 and 2, the polyvinyl alcohol/sodium alginate hydrogel porous composite scaffold containing the bioactive glass with high calcium and phosphorus content has a good porous communication structure. As can be seen from FIG. 3, the growth state of the cells on the composite scaffold is good, and the constructed porous structure of the scaffold is beneficial to the growth of the cells and the transportation of nutrient substances.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. The bone repair material with high calcium and phosphorus content is characterized by comprising bioactive glass powder, sodium alginate and polyvinyl alcohol, wherein the mass ratio of the bioactive glass powder to the sodium alginate to the polyvinyl alcohol is (1-10): (2-8): (8-16); wherein, the molar ratio of the calcium content in the bioactive glass powder is 10-36%; the mol ratio of the phosphorus content is 4-10%.
2. Use of a bone repair material with a high calcium and phosphorus content according to claim 1 for the preparation of a bone repair scaffold.
3. A bone repair scaffold with high calcium and phosphorus content, prepared from the bone repair material of claim 1.
4. A preparation method of a bone repair scaffold with high calcium and phosphorus content is characterized by comprising the following steps:
(1) adding bioactive glass powder into deionized water, and performing ultrasonic and magnetic stirring to uniformly disperse the bioactive glass powder to obtain a suspension A;
(2) adding polyvinyl alcohol into the suspension A, and magnetically stirring in a water bath to uniformly disperse the polyvinyl alcohol to obtain a suspension B;
(3) adding sodium alginate into the suspension B, and magnetically stirring in a water bath to uniformly mix the sodium alginate and the suspension B to obtain a mixed solution C;
(4) and (3) carrying out constant-temperature vacuum treatment on the mixed solution C, pouring the treated mixed solution C into a polytetrafluoroethylene mold plate, pre-freezing and molding at the temperature of-20 ℃, and then carrying out freeze drying to obtain the porous bioactive glass polyvinyl alcohol/sodium alginate hydrogel composite bone scaffold with high calcium and phosphorus content.
5. The method for preparing a bone repair scaffold with high content of calcium and phosphorus according to claim 4, wherein in the step (1), the concentration of the bioactive glass powder in the suspension A is 1-10 w/v%.
6. The method for preparing a bone repair scaffold with high content of calcium and phosphorus according to claim 4, wherein in the step (2), the concentration of the polyvinyl alcohol in the suspension B is 8-16 w/v%.
7. The method for preparing a bone repair scaffold with high content of calcium and phosphorus according to claim 4, wherein the temperature of the water bath in the step (2) and the step (3) is 75-85 ℃.
8. The method for preparing a bone repair scaffold with high calcium and phosphorus content according to claim 4, wherein the concentration of sodium alginate in the mixed solution C in the step (3) is 2-8 w/v%.
9. The method for preparing a bone repair scaffold with high content of calcium and phosphorus according to claim 4, wherein the constant temperature vacuum treatment condition in step (4) is 80 ℃ and the vacuum treatment is 30 min.
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