CN114998376A - Smear sample coating edge scanning defining method and system - Google Patents

Smear sample coating edge scanning defining method and system Download PDF

Info

Publication number
CN114998376A
CN114998376A CN202210563211.0A CN202210563211A CN114998376A CN 114998376 A CN114998376 A CN 114998376A CN 202210563211 A CN202210563211 A CN 202210563211A CN 114998376 A CN114998376 A CN 114998376A
Authority
CN
China
Prior art keywords
smear
image
edge
scanning
image processing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210563211.0A
Other languages
Chinese (zh)
Inventor
王伟佳
陈庆武
胡金龙
董守斌
雷涛
谢文焕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhongshan Yishu Technology Co ltd
Original Assignee
Zhongshan Yishu Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhongshan Yishu Technology Co ltd filed Critical Zhongshan Yishu Technology Co ltd
Priority to CN202210563211.0A priority Critical patent/CN114998376A/en
Publication of CN114998376A publication Critical patent/CN114998376A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/10Segmentation; Edge detection
    • G06T7/13Edge detection
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84Systems specially adapted for particular applications
    • GPHYSICS
    • G06COMPUTING; CALCULATING OR COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection

Abstract

The embodiment of the application discloses a smear sample coating edge scanning defining method and a smear sample coating edge scanning defining system; the method comprises the following steps: shooting the smear through a camera to obtain a smear panoramic image; performing image processing on the smear panoramic image to obtain an edge profile of the smear panoramic image; determining the central point of the edge profile of the smear panoramic image based on the edge profile, and establishing a coordinate system by taking the central point as an origin; the objective lens conversion device is controlled by the controller to convert the objective lens into a high power lens; scanning the smear from the origin to the edge through an objective lens, and stopping scanning at the edge to obtain a scanned picture; splicing and synthesizing the obtained multiple scanned pictures through an image processing device to obtain a target image; the embodiment of the application realizes automatic scanning to obtain the target image, so that the image analysis can be more complete and comprehensive, meanwhile, the scanning time of blank positions can be reduced, and the scanning efficiency is improved.

Description

Smear sample coating edge scanning defining method and system
Technical Field
The embodiment of the application relates to the technical field of smear image processing, in particular to a smear sample coating edge scanning defining method and system.
Background
In order to examine the disease process of organs, tissues or cells, some kind of pathological morphological examination method is often adopted to examine the pathological changes of the organs, tissues or cells, to examine the cause, pathogenesis and pathological change process of the pathological changes, and finally to make pathological diagnosis. In the diagnosis of various diseases, the pathological diagnosis result is the most accurate discrimination result, and is an important index for diagnosing whether the disease is affected in clinical diagnosis.
The pathological morphology examination is carried out by observing the pathological changes of a general specimen, taking a part of the specimen, preparing the specimen into a slide specimen by a histopathology method, and further examining the slide specimen by a microscope. For example, cervical cells are detected and cytological differential diagnosis is performed using a liquid-based thin-layer cell detection system.
In actual examination, a scanner is often used to scan cell information in a sample slide to prepare a digital image for storage and use for examination and labeling work of a doctor, but in the existing sample slide scanning process, a sample slide to be scanned needs to be manually placed on a stage by a worker, and meanwhile, the whole sample slide needs to be scanned during scanning, so that the scanning time is long, and the efficiency is affected.
Disclosure of Invention
The embodiment of the application provides a smear sample coating edge scanning and defining method and system, which are used for solving the problems that the conventional smear sample scanning needs to manually place a sample slide to be scanned on an objective table through manpower, the whole sample slide needs to be scanned during scanning, the scanning time is long, and the efficiency is influenced.
In a first aspect, the present application provides a smear sample coating margin scan defining method, which includes the following steps:
shooting the smear through a camera to obtain a smear panoramic image;
performing image processing on the smear panoramic image through an image processing device to obtain an edge profile of the smear panoramic image;
determining the central point of the edge profile of the smear panoramic image through an image processing device based on the edge profile, and establishing a coordinate system by taking the central point as an origin;
controlling a motor control device through a controller, and controlling an objective lens conversion device to convert an objective lens into a high power lens;
scanning the smear from the origin to the edge through an objective lens, and stopping scanning at the edge to obtain a scanned picture;
and splicing and synthesizing the obtained multiple scanning pictures through an image processing device to obtain a target image.
Further, the image processing the smear panoramic image through the image processing device to obtain the edge profile of the smear panoramic image includes:
converting the smear panoramic image into a gray-scale image through an image processing device;
smoothing the gray level image through a Gaussian smoothing filter;
calculating gradient strength and gradient direction of the gray scale map through finite difference of first order partial derivatives of the gray scale map to generate a global gradient map of the gray scale map;
carrying out non-maximum suppression on each local gradient area in the global gradient map so as to determine edge points in the gray map;
and connecting the edge points by a double threshold value method to form the edge profile curve.
Further, the determining, by an image processing apparatus, a center point of the edge profile of the smear panoramic image based on the edge profile, and establishing a coordinate system with the center point as an origin, includes:
dividing pixels of the smear panorama image into a plurality of grades by an image processing device based on an edge profile, wherein each grade comprises at least 3 pixels;
determining the central point of each image according to the pixel distribution central points of a plurality of levels of the smear panoramic image, wherein the sum of the distance variances between the pixel distribution central point of any level of any image and each pixel of the level in the image is minimum, and the coordinate of the central point of any image is equal to the coordinate average value of each pixel distribution central point of the image;
and establishing a coordinate system by taking the determined central point as an origin.
Further, the scanning smear is scanned from the origin to the edge through the objective lens, and the scanning is stopped at the edge, so as to obtain a scanning picture, including:
and scanning the smear to the edge by taking the determined central point as an origin, continuing to scan the preset position after scanning to the edge contour, and stopping scanning to obtain a scanned picture.
Further, the splicing and synthesizing the obtained multiple scanned pictures through the image processing device to obtain the target image includes:
splicing the obtained multiple scanned pictures through an image processing device to generate a spliced picture preview frame, and comparing the spliced picture preview frame with a preset target picture frame;
and if the spliced picture preview frame is consistent with the preset target picture frame, outputting the spliced picture to obtain the scanned panoramic picture.
Further, before the image processing device performs image processing on the smear panoramic image and obtains an edge profile of the smear panoramic image, the method further includes:
image preprocessing is carried out on the smear panoramic image through an image processing device; wherein the image preprocessing comprises cropping the smear panoramic image to make the size of the smear panoramic image within an initial image setting value.
In a second aspect, the present application further provides a smear sample coating margin scan defining system, including: the device comprises an object stage, a microscope, a motor control device, an objective lens conversion device, an objective lens, a camera, an image processing device and a controller;
the objective table is used for bearing the smear; the motor control device is connected with the object stage and used for controlling the object stage to move in a three-dimensional space; the microscope is used for observing the smear; the camera is used for shooting the smear to obtain a smear panoramic image; the image processing device is used for carrying out image processing on the smear panoramic image; the controller is used for controlling the motor control device and controlling the objective lens conversion device to convert the objective lens into the high power lens; the objective lens is used for scanning smears.
Furthermore, the device also comprises a detection sensor, an LED illuminating lamp, a camera driving device and an image transmission device; the detection sensor, the LED illuminating lamp and the camera driving device are connected with the controller, the camera driving circuit is connected with the camera and the image transmission device, and the image transmission device is connected with the image processing device; the detection sensor is used for detecting the smear; the LED illuminating lamp is used for illuminating the smear; the camera driving device is used for driving the camera; the image transmission device is used for collecting the smear panoramic image shot by the camera and transmitting the smear panoramic image to the image processing device.
In a third aspect, an embodiment of the present application further provides a computer device, including: a memory and one or more processors;
the memory for storing one or more programs;
when executed by the one or more processors, cause the one or more processors to implement a smear sample coating margin scan bounding method as described above.
In a fourth aspect, embodiments of the present application also provide a storage medium containing computer executable instructions for performing a smear sample coating margin scan defining method as described above when executed by a computer processor.
In the embodiment of the application, the smear is shot by the camera to obtain a smear panoramic image; performing image processing on the smear panoramic image through an image processing device to obtain an edge profile of the smear panoramic image; determining the central point of the edge profile of the smear panoramic image through an image processing device based on the edge profile, and establishing a coordinate system by taking the central point as an origin; the motor control device is controlled by a controller, and the objective lens conversion device is controlled to convert the objective lens into a high power lens; scanning the smear from the origin to the edge through an objective lens, and stopping scanning at the edge to obtain a scanned picture; splicing and synthesizing the obtained multiple scanned pictures through an image processing device to obtain a target image; the target image is obtained through automatic scanning, so that the image analysis can be more complete and comprehensive, meanwhile, the scanning time of blank positions can be reduced, and the scanning efficiency is improved.
Drawings
FIG. 1 is a flowchart of a smear sample coating margin scan defining method provided by an embodiment of the present application;
FIG. 2 is a schematic structural diagram of a smear sample coating margin scan defining system provided by an embodiment of the present application;
fig. 3 is a schematic structural diagram of a computer device according to an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, specific embodiments of the present application will be described in detail with reference to the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the application and are not limiting of the application. It should be further noted that, for the convenience of description, only some but not all of the relevant portions of the present application are shown in the drawings. Before discussing exemplary embodiments in greater detail, it should be noted that some exemplary embodiments are described as processes or methods depicted as flowcharts. Although a flowchart may describe the operations (or steps) as a sequential process, many of the operations can be performed in parallel, concurrently or simultaneously. In addition, the order of the operations may be re-arranged. The process may be terminated when its operations are completed, but may have additional steps not included in the figure. The processes may correspond to methods, functions, procedures, subroutines, and the like.
At present, in actual examination, a scanner is commonly used to scan cell information in a sample slide to prepare a digital image for storage and use for examination and labeling work of a reading doctor, but in the existing sample slide scanning process, a sample slide to be scanned needs to be manually placed on a stage by manpower, and meanwhile, the whole sample slide needs to be scanned during scanning, so that the scanning time is long, and the efficiency is affected. The embodiment of the application establishes a smear sample coating edge scanning and defining method to solve the problems that the conventional smear sample scanning needs to manually place a sample slide to be scanned on an objective table through manpower, the whole sample slide needs to be scanned during scanning, the scanning time is long, and the efficiency is influenced.
The smear sample coating margin scan defining method provided in the embodiment can be executed by a smear sample coating margin scan defining system, which can be implemented by means of software and/or hardware and is integrated in a smear sample coating margin scan defining device. Wherein, the smear sample coating edge scanning and defining device can be a computer or the like.
FIG. 1 is a flowchart of a smear sample coating margin scan defining method provided by the embodiment of the present application. Referring to fig. 1, the method comprises the steps of:
and step 110, shooting the smear through a camera to obtain a smear panoramic image.
Specifically, the detection sensor detects that a smear exists on the objective table, starts a starting signal to the controller, controls the brightness of the LED illuminating lamp to be opened and controlled through the controller, and controls the camera to shoot the smear to obtain a smear panoramic image.
Optionally, the sizes of the smears of different types are different, the smears of different sizes are detected through the detection sensor, corresponding detection signals are sent to the controller, the controller calls a light scheme corresponding to the type of the smears from the storage according to the detection signals, and the brightness of the LED illuminating lamp is controlled according to the light scheme.
Optionally, after obtaining the smear panoramic image, performing image preprocessing on the smear panoramic image through an image processing device; wherein the image preprocessing comprises cropping the smear panoramic image so that the size of the smear panoramic image is within an initial image setting value; it can be understood that the initial image setting values corresponding to different types of smears are different, the controller sends the smear type to the image processing device, and the image processing device calls the corresponding initial image setting value according to the smear type and cuts the smear panoramic image so that the size of the smear panoramic image is within the corresponding initial image setting value; it should be noted that the initial image setting value may be set according to a specific smear, which is not limited in this embodiment of the application.
And step 120, carrying out image processing on the smear panoramic image through an image processing device to obtain an edge profile of the smear panoramic image.
Specifically, the smear panoramic image is converted into a gray-scale image through an image processing device; smoothing the gray level image through a Gaussian smoothing filter; calculating gradient strength and gradient direction of the gray scale map by finite difference of first order partial derivatives of the gray scale map to generate a global gradient map of the gray scale map; carrying out non-maximum suppression on each local gradient area in the global gradient map so as to determine edge points in the gray map; and connecting the edge points by a double threshold value method to form the edge profile curve.
Optionally, non-maximum suppression is performed on each local gradient region in the global gradient map, and the edge point in the gray map is determined and the discrete response caused by edge detection is eliminated by retaining the pixel value of the maximum point in the local gradient and setting the gray value of the point corresponding to the non-maximum point to 0.
Optionally, the real edge points in the gray-scale image are further extracted by a double-threshold method, and the real edge points processed by the double-threshold method are connected to form an edge contour curve. The double-threshold method is characterized in that two thresholds are set, namely a high threshold and a low threshold, edge points with pixel point intensity values larger than the high threshold are used as real edge points, and edge points with pixel point intensity values smaller than the low threshold are used as non-real edge points; and if the intensity value of the adjacent pixel point is greater than the high threshold value, the edge point is taken as a real edge point, otherwise, the edge point is taken as a non-real edge point.
And step 130, determining the central point of the edge contour of the smear panoramic image through an image processing device based on the edge contour, and establishing a coordinate system by taking the central point as an origin.
Specifically, based on the edge profile, dividing pixels of the smear panoramic image into a plurality of levels by an image processing device, wherein each level comprises at least 3 pixels; determining the central point of each image according to the pixel distribution central points of a plurality of levels of the smear panoramic image, wherein the sum of the distance variances between the pixel distribution central point of any level of any image and each pixel of the level in the image is minimum, and the coordinate of the central point of any image is equal to the coordinate average value of each pixel distribution central point of the image; and establishing a coordinate system by taking the determined central point as an origin.
And step 140, controlling the motor control device through the controller, and controlling the objective lens conversion device to convert the objective lens into the high-power lens.
Specifically, the objective lens conversion device is controlled to make the high power objective lens aim at the objective table, the coordinate range of the motor control device for controlling the movement of the objective table is calculated according to the relation between the low power objective lens and the high power objective lens, and the motor control device is controlled to control the objective table by setting the stepping distance, so that the objective table moves in the coordinate range.
And 150, scanning the smear from the origin to the edge through the objective lens, and stopping scanning at the edge to obtain a scanned picture.
Specifically, the smear is scanned towards the edge by taking the determined central point as an origin, and after the smear is scanned to the edge contour, the scanning is stopped after the scanning is continued to the preset position, so that a scanning picture is obtained.
And step 160, splicing and synthesizing the obtained multiple scanning pictures through the image processing device to obtain a target image.
Specifically, a plurality of obtained scanned pictures are spliced through an image processing device to generate a spliced picture preview frame, and the spliced picture preview frame is compared with a preset target picture frame; if the spliced picture preview frame is consistent with a preset target picture frame, outputting a spliced picture to obtain a scanned panoramic picture; it can be understood that the target picture frame can be set according to the specific type of the smear, and the size, shape and other characteristics of the target picture frame are not limited in the embodiment of the application.
On the basis of the above embodiments, fig. 2 is a schematic structural diagram of a smear sample coating edge scanning defining system provided by the embodiment of the present application. Referring to fig. 2, the smear sample coating boundary scan defining system provided in the present embodiment specifically includes: an object stage 201, a microscope 202, a motor control device, an objective lens conversion device, an objective lens 203, a camera 204, an image processing device and a controller;
wherein the object stage is used for bearing the smear; the motor control device is connected with the object stage and is used for controlling the object stage to move in a three-dimensional space; the microscope is used for observing the smear; the camera is used for shooting the smear to obtain a smear panoramic image; the image processing device is used for carrying out image processing on the smear panoramic image; the controller is used for controlling the motor control device and controlling the objective lens conversion device to convert the objective lens into the high power lens; the objective lens is used for scanning smears.
In some embodiments, the system further comprises a detection sensor, an LED illuminating lamp, a camera driving device and an image transmission device; the detection sensor, the LED illuminating lamp and the camera driving device are connected with the controller, the camera driving circuit is connected with the camera and the image transmission device, and the image transmission device is connected with the image processing device; the detection sensor is used for detecting the smear; the LED illuminating lamp is used for illuminating the smear; the camera driving device is used for driving the camera; the image transmission device is used for collecting the smear panoramic image shot by the camera and transmitting the smear panoramic image to the image processing device.
Shooting the smear through a camera to obtain a smear panoramic image; performing image processing on the smear panoramic image through an image processing device to obtain an edge profile of the smear panoramic image; determining the central point of the edge profile of the smear panoramic image through an image processing device based on the edge profile, and establishing a coordinate system by taking the central point as an origin; the motor control device is controlled by a controller, and the objective lens conversion device is controlled to convert the objective lens into a high power lens; scanning the smear from the origin to the edge through an objective lens, and stopping scanning at the edge to obtain a scanned picture; splicing and synthesizing the obtained multiple scanned pictures through an image processing device to obtain a target image; the target image is obtained through automatic scanning, so that the image analysis can be more complete and comprehensive, meanwhile, the scanning time of blank positions can be reduced, and the scanning efficiency is improved.
The smear sample coating edge scanning and defining system provided by the embodiment of the application can be used for executing the smear sample coating edge scanning and defining method provided by the embodiment, and has corresponding functions and beneficial effects.
The embodiment of the application also provides computer equipment which can integrate the smear sample coating edge scanning and defining system provided by the embodiment of the application. Fig. 3 is a schematic structural diagram of a computer device according to an embodiment of the present application. Referring to fig. 3, the computer apparatus includes: an input device 43, an output device 34, a memory 32, and one or more processors 31; the memory 32 for storing one or more programs; when executed by the one or more processors 31, cause the one or more processors 31 to implement the smear sample coating margin scan definition method as provided in the embodiments above. The input device 33, the output device 34, the memory 32 and the processor 31 may be connected by a bus or other means, and fig. 3 illustrates the connection by the bus as an example.
The processor 31 executes various functional applications of the device and data processing by running software programs, instructions and modules stored in the memory 31, namely, the smear sample coating edge scanning defining method is realized.
The computer equipment provided by the above can be used for executing the smear sample coating edge scanning and defining method provided by the above embodiment, and has corresponding functions and beneficial effects.
Embodiments of the present application also provide a storage medium containing computer executable instructions, which when executed by a computer processor, perform a method for smear sample coating margin scan delineation, the method comprising: the camera shoots the smear to obtain a smear panoramic image; performing image processing on the smear panoramic image through an image processing device to obtain an edge profile of the smear panoramic image; determining the central point of the edge profile of the smear panoramic image through an image processing device based on the edge profile, and establishing a coordinate system by taking the central point as an origin; the motor control device is controlled by a controller, and the objective lens conversion device is controlled to convert the objective lens into a high power lens; scanning the smear from the origin to the edge through an objective lens, and stopping scanning at the edge to obtain a scanned picture; and splicing and synthesizing the obtained multiple scanning pictures through an image processing device to obtain a target image.
Storage medium-any of various types of memory devices or storage devices. The term "storage medium" is intended to include: mounting media such as CD-ROM, floppy disk, or tape devices; computer device memory or random access memory such as DRAM, DDR RAM, SRAM, EDO RAM, Lanbas (Rambus) RAM, etc.; non-volatile memory such as flash memory, magnetic media (e.g., hard disk or optical storage); registers or other similar types of memory elements, etc. The storage medium may also include other types of memory or combinations thereof. In addition, the storage medium may be located in a first computer apparatus in which the program is executed, or may be located in a different second computer apparatus connected to the first computer apparatus through a network (such as the internet). The second computer device may provide program instructions to the first computer for execution. The term "storage medium" may include two or more storage media that may reside in different locations, such as in different computer devices that are connected by a network. The storage medium may store program instructions (e.g., embodied as a computer program) that are executable by one or more processors.
Of course, the storage medium provided by the embodiments of the present application contains computer executable instructions, and the computer executable instructions are not limited to the smear sample coating edge scan defining method described above, but also can perform related operations in the smear sample coating edge scan defining method provided by any embodiments of the present application.
The smear sample coating margin scan defining system, the storage medium and the computer device provided in the above embodiments can execute the smear sample coating margin scan defining method provided in any embodiment of the present application, and the technical details not described in detail in the above embodiments can be referred to the smear sample coating margin scan defining method provided in any embodiment of the present application.
The foregoing is considered as illustrative of the preferred embodiments of the invention and the technical principles employed. The present application is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present application has been described in more detail with reference to the above embodiments, the present application is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present application, and the scope of the present application is determined by the scope of the claims.

Claims (10)

1. A method for scanning and defining the coating edge of a smear sample, which is characterized by comprising the following steps:
shooting the smear through a camera to obtain a smear panoramic image;
performing image processing on the smear panoramic image through an image processing device to obtain an edge profile of the smear panoramic image;
determining the central point of the edge profile of the smear panoramic image through an image processing device based on the edge profile, and establishing a coordinate system by taking the central point as an origin;
controlling a motor control device through a controller, and controlling an objective lens conversion device to convert an objective lens into a high-power lens;
scanning the smear from the origin to the edge through an objective lens, and stopping scanning at the edge to obtain a scanned picture;
and splicing and synthesizing the obtained multiple scanning pictures through an image processing device to obtain a target image.
2. The smear sample coating edge scan defining method according to claim 1, wherein the image processing the smear panoramic image by an image processing device to obtain the edge profile of the smear panoramic image comprises:
converting the smear panoramic image into a gray-scale image through an image processing device;
smoothing the gray level image through a Gaussian smoothing filter;
calculating gradient strength and gradient direction of the gray scale map by finite difference of first order partial derivatives of the gray scale map to generate a global gradient map of the gray scale map;
carrying out non-maximum suppression on each local gradient area in the global gradient map so as to determine edge points in the gray map;
and connecting the edge points by a double threshold value method to form the edge profile curve.
3. The smear sample coating edge scan defining method according to claim 1, wherein the determining a center point of the edge profile of the smear panoramic image by an image processing device based on the edge profile, with the center point as an origin, establishing a coordinate system, comprises:
dividing pixels of the smear panorama image into a plurality of grades by an image processing device based on an edge profile, wherein each grade comprises at least 3 pixels;
determining the central point of each image according to pixel distribution central points of a plurality of levels of the smear panoramic image, wherein the sum of the distance variance between the pixel distribution central point of any level of any image and each pixel of the level in the image is minimum, and the coordinate of the central point of any image is equal to the coordinate average value of the pixel distribution central points of the image;
and establishing a coordinate system by taking the determined central point as an origin.
4. The smear sample coating margin scan defining method of claim 3, wherein the scanning is stopped from the origin to the margin by the objective lens, and the scanning picture is obtained, comprising:
and scanning the smear to the edge by taking the determined central point as an origin, continuing to scan the preset position after scanning to the edge contour, and stopping scanning to obtain a scanned picture.
5. The smear sample coating edge scanning and defining method of claim 1, wherein the stitching and combining the obtained multiple scanning pictures by the image processing device to obtain the target image comprises:
splicing the obtained multiple scanned pictures through an image processing device to generate a spliced picture preview frame, and comparing the spliced picture preview frame with a preset target picture frame;
and if the spliced picture preview frame is consistent with the preset target picture frame, outputting the spliced picture to obtain the scanned panoramic picture.
6. The smear sample coating edge scan defining method according to claim 1, wherein before the smear panoramic image is image processed by an image processing device to obtain the edge profile of the smear panoramic image, further comprising:
image preprocessing is carried out on the smear panoramic image through an image processing device; wherein the image preprocessing comprises cropping the smear panoramic image so that the size of the smear panoramic image is within an initial image setting value.
7. A smear sample coating margin scan bounding system comprising: the device comprises an object stage, a microscope, a motor control device, an objective lens conversion device, an objective lens, a camera, an image processing device and a controller;
the objective table is used for bearing the smear; the motor control device is connected with the object stage and used for controlling the object stage to move in a three-dimensional space; the microscope is used for observing the smear; the camera is used for shooting the smear to obtain a smear panoramic image; the image processing device is used for carrying out image processing on the smear panoramic image; the controller is used for controlling the motor control device and controlling the objective lens conversion device to convert the objective lens into the high power lens; the objective lens is used for scanning smears.
8. The smear sample coating edge scan defining system of claim 7, further comprising a detection sensor, an LED illumination lamp, a camera driving device and an image transmission device; the detection sensor, the LED illuminating lamp and the camera driving device are connected with the controller, the camera driving circuit is connected with the camera and the image transmission device, and the image transmission device is connected with the image processing device; the detection sensor is used for detecting the smear; the LED illuminating lamp is used for illuminating the smear; the camera driving device is used for driving the camera; the image transmission device is used for collecting the smear panoramic image shot by the camera and transmitting the smear panoramic image to the image processing device.
9. A computer device, comprising: a memory and one or more processors;
the memory for storing one or more programs;
when executed by said one or more processors, cause said one or more processors to implement a method for smear sample coating margin scan definition according to any of claims 1-6.
10. A storage medium containing computer executable instructions for performing a method of smear sample coating margin scan definition according to any of claims 1-6 when executed by a computer processor.
CN202210563211.0A 2022-05-19 2022-05-19 Smear sample coating edge scanning defining method and system Pending CN114998376A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210563211.0A CN114998376A (en) 2022-05-19 2022-05-19 Smear sample coating edge scanning defining method and system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210563211.0A CN114998376A (en) 2022-05-19 2022-05-19 Smear sample coating edge scanning defining method and system

Publications (1)

Publication Number Publication Date
CN114998376A true CN114998376A (en) 2022-09-02

Family

ID=83027518

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210563211.0A Pending CN114998376A (en) 2022-05-19 2022-05-19 Smear sample coating edge scanning defining method and system

Country Status (1)

Country Link
CN (1) CN114998376A (en)

Similar Documents

Publication Publication Date Title
US11721018B2 (en) System and method for calculating focus variation for a digital microscope
RU2595495C2 (en) Image processing device, image processing method and image processing system
JP6086949B2 (en) Image analysis method based on chromogen separation
US10890576B2 (en) Image processing device, image processing method, and recording medium
JP5996334B2 (en) Microscope system, specimen image generation method and program
CA2826372A1 (en) Fast auto-focus in microscopic imaging
CN111931751B (en) Deep learning training method, target object identification method, system and storage medium
US20120249770A1 (en) Method for automatically focusing a microscope on a predetermined object and microscope for automatic focusing
JPWO2017150194A1 (en) Image processing apparatus, image processing method, and program
CN115047610B (en) Chromosome karyotype analysis device and method for automatically fitting microscopic focusing plane
CN114663446A (en) System and method for segmentation of three-dimensional microscope images
CN111679418B (en) Microscope automatic focusing method and system based on laser image and computer equipment
CN110363734B (en) Thick sample microscopic fluorescence image reconstruction method and system
JP2015114172A (en) Image processing apparatus, microscope system, image processing method, and image processing program
CN109001902A (en) Microscope focus method based on image co-registration
CN113533372B (en) Paper defect detection method, system, device and computer storage medium
CN109003228B (en) Dark field microscopic large-view-field automatic stitching imaging method
CN114998376A (en) Smear sample coating edge scanning defining method and system
CN115079393A (en) Chromosome karyotype analysis microscopic shooting device and method based on 10-fold objective lens
US20200074628A1 (en) Image processing apparatus, imaging system, image processing method and computer readable recoding medium
JP5648366B2 (en) Microscope control apparatus and region determination method
CN112363309A (en) Automatic focusing method and system for pathological image under microscope
JP5861678B2 (en) Image processing apparatus, program, and image processing system
CN112541939B (en) Method and device for acquiring clear focal plane position of sample
CN114354607B (en) Luminosity three-dimensional flaw detection method based on spiral phase contrast filtering algorithm

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination