CN114995559A - Control system and control method of intelligent biological sample repository - Google Patents
Control system and control method of intelligent biological sample repository Download PDFInfo
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- 239000012472 biological sample Substances 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 34
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 335
- 239000007788 liquid Substances 0.000 claims abstract description 258
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 167
- 239000007789 gas Substances 0.000 claims abstract description 43
- 238000001514 detection method Methods 0.000 claims abstract description 37
- 230000001502 supplementing effect Effects 0.000 claims abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 27
- 239000001301 oxygen Substances 0.000 claims description 27
- 229910052760 oxygen Inorganic materials 0.000 claims description 27
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- 239000013589 supplement Substances 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 4
- 238000001802 infusion Methods 0.000 claims description 4
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- 239000002184 metal Substances 0.000 claims description 3
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- 239000000523 sample Substances 0.000 abstract 1
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- 238000007710 freezing Methods 0.000 description 7
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- 201000011510 cancer Diseases 0.000 description 1
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- 125000004122 cyclic group Chemical group 0.000 description 1
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- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D27/00—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
- G05D27/02—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention discloses a control system and a control method of an intelligent biological sample repository, which comprises a biological sample repository, an automatic liquid nitrogen supplementing system and an environment control system, wherein the biological sample repository comprises a console, an exhaust device, a liquid nitrogen supply pipeline, a liquid nitrogen supply tank, a temperature and humidity control device, a gas concentration detection module, a liquid nitrogen container, a low-temperature electromagnetic valve and an exhaust valve; the liquid nitrogen automatic liquid supplementing system comprises a core chip module, a liquid level acquisition module, a power supply module, a display module, a key module and an electromagnetic valve driving module; the environment control system comprises a core chip module, a programmable temperature control module, a power supply module, a display module, a key module, an exhaust fan driving module and a gas concentration detection device; the invention plays a positive role in the reliability and long-term stability of the sample low-temperature preservation.
Description
Technical Field
The invention relates to the field of biological sample storage, in particular to a control system and a control method of an intelligent biological sample storage warehouse.
Background
Since the 21 st century, with the rapid development of modern biotechnology and research, subjects such as bioscience, medicine and molecular genetics have advanced sufficiently, and the protection and development of human genetic resources abroad are strong, wherein the protection and development are embodied by the large-scale construction of a genetic resource base on the premise of standardization, and the genetic resource base is also called a biological sample repository. China also increases the attention degree on the construction of biological sample repositories, and obtains certain achievements after spending a large amount of manpower and material resources in this aspect. At present, biological sample storage in the promotion of blood diseases, immune system diseases, genetic diseases and cancer serious diseases scientific research play an indispensable role.
The traditional method for storing biological samples at low temperature mostly adopts an ultra-low temperature refrigerator and a single liquid nitrogen storage tank. The traditional biological sample low-temperature storage method belongs to single independent unit storage, and has the limitations of low control system reliability, difficult management, poor safety and the like for long-term concentrated storage of a large number of biological samples. Among methods for preserving a large number of biological samples for a long time, the method of freezing by liquid nitrogen is becoming a more and more popular method. In the existing biological sample storage warehouse frozen by liquid nitrogen, the product price of domestic manufacturers is low, but certain problems exist in the aspect of a control system, and the problems mainly comprise that the detection method of the liquid level height of the liquid nitrogen is incomplete, the function of the control system is incomplete, the use of a user is inconvenient, and the like; products of foreign manufacturers are improved in the aspect of control system functions, but have the problems of high price, difficulty in maintenance and the like. How to ensure that the biological sample repository can work for a long time, effectively, and automatically and intelligently has become the key point of the design and research of the biological sample repository.
Disclosure of Invention
The method aims to solve the problems of the long-term centralized preservation of a large number of biological samples, low reliability of a control system, difficult management, poor safety and the like in the background art and solve the problems of the control system of the conventional biological sample storage warehouse, such as incomplete liquid nitrogen liquid level detection method, incomplete control system function and the like. The invention provides a control system and a control method for an intelligent biological sample repository. The invention designs an automatic liquid supplementing system aiming at the biological storage tank so as to ensure the cryopreservation effect inside the biological storage tank; aiming at the storage environment, the proportion of overhigh nitrogen in the air is reduced by utilizing the ventilation function, so that the personnel can conveniently enter and exit, and the storage device is safer.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the utility model provides a control system of intelligence biological sample repository, includes biological sample repository, the automatic fluid infusion system of liquid nitrogen and environmental control system, its characterized in that: the biological sample storage warehouse comprises a control console, an exhaust device, a liquid nitrogen supply pipeline, a liquid nitrogen supply tank, a temperature and humidity control device, a gas concentration detection module, a liquid nitrogen container, a low-temperature electromagnetic valve and an exhaust valve; the upper end of the liquid nitrogen container is connected with a low-temperature electromagnetic valve, the low-temperature electromagnetic valve is connected with a branch pipeline of a liquid nitrogen supply pipeline through a low-temperature metal hose, and when the automatic control of the low-temperature electromagnetic valve fails, an operator can manually operate a valve to complete the opening and closing of the low-temperature electromagnetic valve; the branch pipeline of the liquid nitrogen supply pipeline is welded with a matched exhaust valve, the exhaust valve is used for exhausting gas generated by volatilization of liquid nitrogen in the transportation process, the internal pressure can be reduced through the exhausted gas, and the liquid nitrogen supply pipeline is connected with a liquid nitrogen supply tank. The main liquid nitrogen pipeline is mainly arranged outside the room, a liquid nitrogen conveying line is mainly arranged above the ceiling, and the part inside the room is a branch pipe welded with each liquid nitrogen container through one main pipeline.
The liquid nitrogen automatic liquid supplementing system comprises a core chip module, a liquid level acquisition module, a power supply module, a display module, a key module and an electromagnetic valve driving module; the liquid level acquisition module comprises a capacitance type liquid level sensor, an analog-to-digital converter and a single chip microcomputer.
The environment control system comprises a core chip module, a programmable temperature control module, a power supply module, a display module, a key module, an exhaust fan driving module and a gas concentration detection device; the exhaust fan driving module comprises an exhaust device and a driving motor; the gas concentration detection module comprises a gas concentration sensor, an alarm lamp and an alarm.
Furthermore, the two exhaust devices are respectively arranged on two walls close to the outside, so that the two exhaust devices can exchange with the outside air conveniently, and the concentration of nitrogen in the indoor air is reduced.
Furthermore, the number of the gas concentration detection modules is three, and the gas concentration detection modules are respectively arranged at the centers of the three walls. At 40-60m 2 The three gas concentration sensors can just cover the whole room, and the concentrations of nitrogen and oxygen can be effectively monitored.
Furthermore, the temperature and humidity control devices are four and are respectively placed at four corners close to the wall, so that the temperature and the humidity in the biological sample storage warehouse can be adjusted, and the air exchange of the exhaust device can be assisted to a certain degree.
Further, the control method of the control system of the intelligent biological sample repository is characterized in that: the method comprises the following steps:
s1: starting an intelligent biological sample repository control system;
s2: starting an automatic liquid supplementing system of liquid nitrogen;
s3: starting an environment control system;
further, the liquid nitrogen container can reduce the freezing effect on the biological sample due to the excessively low liquid nitrogen level, and the biological sample can deteriorate due to the reduction of the freezing effect in the temperature rise process, so that an automatic liquid supplementing control system is needed to supplement the liquid nitrogen when the liquid nitrogen level of the liquid nitrogen container is lower than the safety value of the biological sample for freezing. The working steps of the automatic liquid supplementing system for the liquid nitrogen comprise:
s1: setting the liquid level height according to the requirement, namely obtaining a preset value, wherein 80% of the preset value is a safety value for freezing and storing the biological sample;
s2: collecting liquid level signals for the liquid nitrogen container through a liquid level collecting module, performing analog-to-digital conversion on the signals, displaying the signals through a liquid level display module, and performing on-off judgment and on-off time judgment on the normally closed low-temperature electromagnetic valve according to the converted liquid level data;
s3: when the liquid level detection module detects that the liquid level of the liquid nitrogen container is lower than 80% of a preset value, low-level alarming is carried out, and at the moment, a pin on the chip outputs a 3.3V high-level signal, so that a low-temperature electromagnetic valve corresponding to the liquid nitrogen container is opened, and the liquid nitrogen starts to be filled;
s4: along with the continuous filling of liquid nitrogen, the liquid level of the liquid nitrogen in the liquid nitrogen container starts to rise, and when the liquid level detection module detects that the liquid level of the liquid nitrogen container for liquid supplement is greater than or equal to 95% of a preset value, a pin corresponding to the low-temperature electromagnetic valve outputs 0V low level and closes the low-temperature electromagnetic valve;
the liquid level acquisition module of the automatic liquid supplementing system for the liquid nitrogen detects the liquid level of the liquid nitrogen by using the capacitive liquid level sensor, and the liquid level is measured according to the change of capacitance between parallel polar plates, wherein the calculation formula of the capacitance C is as follows:
in the formula: w is the width of the electrode plate; h is the height of the electrode plate; h is x Is the liquid height between the electrode plates; d is the electrode plate spacing; epsilon x Is the relative dielectric constant of the liquid medium between the electrode plates; epsilon 0 Is the relative dielectric constant of the gaseous medium between the electrode plates.
According to the relation among the measured capacitance, the liquid level height and the preset dielectric constant, the signal transmitter can obtain a calculation formula of the liquid level height of the liquid nitrogen as follows:
in the formula: epsilon Preparation of Is a predetermined relative dielectric constant of the liquid medium between the electrode plates.
And finally, converting the liquid level height of the liquid nitrogen into a volume value of the liquid nitrogen, and outputting the volume value to a display module for real-time display. The conversion formula of the liquid level height and the volume value of the liquid nitrogen is as follows:
in the formula: and R is the radius of the inner wall of the liquid nitrogen container.
Since the boiling point of liquid nitrogen is-196 ℃, the liquid nitrogen can be boiled violently at normal temperature and normal pressure, and a part of the liquid nitrogen splashes onto the capacitance type liquid level sensor. In the process of supplying liquid nitrogen, the liquid level of the liquid nitrogen gradually rises, the liquid nitrogen which is violently boiled and the liquid nitrogen which is continuously supplied can splash onto the capacitance type liquid level sensor, continuous impact force is generated, the measured value of the liquid nitrogen level of the capacitance type sensor deviates from the true value for a long time, the monitoring of the liquid nitrogen level is interfered, and a false liquid level is formed. After the low-temperature electromagnetic valve is closed, the actual value of the liquid nitrogen liquid level is not the value measured by the sensor, and the liquid level is falsely high.
Aiming at the possibility, the invention adopts a liquid nitrogen circulating supplement method, when liquid nitrogen level data sensed by a capacitive sensor meets more than 95% of a preset value, a chip pin outputs a low level signal of 0V, so that a low-temperature electromagnetic valve is in a continuously closed state, and the liquid nitrogen level signal is continuously acquired. After the liquid nitrogen supply is stopped, when the liquid nitrogen level data collected by the capacitance sensor is less than 80% of the preset value in a short time, the pin outputs a high-level signal of 3.3V, and the low-temperature electromagnetic valve is opened again. The method for circularly replenishing the liquid nitrogen can relieve the liquid level false height caused by false liquid level to a certain extent, and when the circulating liquid supply is stopped, the liquid nitrogen replenishing is finished.
Further, the working steps of the environment control system comprise the control of gas concentration, the control of environment temperature and the control of environment humidity:
the biological sample storage warehouse adopts a liquid nitrogen freezing storage mode in the process of storing the biological sample. Although various liquid nitrogen equipment has the requirements of vacuum, heat insulation and high sealing, absolute vacuum, heat insulation and sealing do not exist, and liquid nitrogen still evaporates. As the biological sample repository operates, the nitrogen content continues to increase, and the oxygen concentration therefore decreases, which in turn may lead to breathing difficulties and even asphyxiation by workers in the area. The biological specimen storage is provided with good ventilation and ventilation facilities to ensure personnel safety. Therefore, a gas concentration control system is designed.
Working steps for gas concentration control:
s1: setting the initial value of the oxygen concentration to be 21% and the initial value of the nitrogen to be 78%, presetting the oxygen concentration corresponding to each exhaust grade, and dividing the wind speed into 3 grades of low, medium and strong exhaust;
s2: acquiring an oxygen concentration signal through a gas concentration detection module;
s3: converting the acquired oxygen concentration signal into concentration data, and displaying the concentration data through a display module;
s4: starting low-grade exhaust when the detected oxygen concentration in the liquid nitrogen reservoir is more than 20% and less than or equal to 21%; when the oxygen concentration is more than 19% and less than or equal to 20%, starting middle-grade exhaust, and starting an alarm lamp and an alarm; when the oxygen concentration is less than or equal to 19 percent, strong-grade exhaust is started, and an alarm lamp and an alarm are turned on, so that the aim of reducing the nitrogen concentration in the air is fulfilled, and the multi-grade exhaust control also realizes energy conservation to a certain extent; the alarm lamp and the alarm are used for reminding working personnel in the biological sample storage warehouse to rapidly leave when the nitrogen concentration in the biological sample storage warehouse is too high;
s5: after exhausting for 20min, the exhaust device, the alarm lamp and the alarm are automatically turned off.
The environmental temperature of the biological sample storage warehouse is required to be 15-28 ℃, and the environmental humidity of the biological sample storage warehouse is less than or equal to 40%, so that the environmental temperature and the environmental humidity need to be controlled.
Working steps for ambient temperature control:
s1: setting the initial environment temperature to be 15-28 ℃;
s2: detecting the ambient temperature;
s3: displaying the detection temperature, and processing:
when the ambient temperature is more than or equal to 28 ℃, the air conditioner opens the refrigeration mode to reduce the ambient temperature to the range of the initial value; when the ambient temperature is less than or equal to 15 ℃, the air conditioner opens the heating mode to increase the ambient temperature to the range of the initial value; when the ambient temperature reaches the range of the initial value, the air conditioner is in a low-power constant-temperature mode;
the working steps of controlling the environmental humidity are as follows:
s1: setting the initial environment humidity to be less than or equal to 40 percent;
s2: detecting the environmental humidity;
s3: displaying the detection temperature, and processing:
when the ambient humidity is more than 40%, the dehumidification function is started; and when the ambient humidity is less than or equal to 40%, the dehumidification function is closed.
The invention has the following advantages and beneficial effects:
(1) the control system and the control method of the intelligent biological sample storage warehouse play an active role in the reliability and long-term stability of low-temperature storage of samples, wherein the automatic liquid nitrogen supplementing system ensures long-term stable and effective work in a mode of periodically adding liquid nitrogen;
(2) the invention provides a perfect control system, which comprises an automatic liquid nitrogen replenishing system and an environment control system, wherein the environment control system also comprises the control of gas concentration, the control of environment temperature and the control of environment humidity, so that the working efficiency of a biological sample storage warehouse can be effectively improved;
(3) compared with the existing biological sample storage warehouse, the biological sample storage warehouse is convenient to maintain, and can work for a long time, effectively, automatically and intelligently;
(4) the environment control system can effectively ensure that the working personnel work in a safe environment, wherein the gas concentration control system aims to remind the working personnel in the biological sample storage warehouse to rapidly leave when the nitrogen concentration in the biological sample storage warehouse is too high, and the working personnel can observe the oxygen concentration content and the nitrogen concentration content in the biological sample storage warehouse through the control system operation interface on a computer, can also detect whether the exhaust device is started through the sightseeing window on the door of the biological sample storage warehouse, and can forcibly start the exhaust device if the exhaust device is not started in time.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a side view of the liquid nitrogen container of FIG. 1 of the present invention;
FIG. 3 is a block diagram of the automatic fluid infusion control system of the present invention;
FIG. 4 is a block flow diagram of a module for level detection according to the present invention;
FIG. 5 is a block flow diagram of a cyclic liquid nitrogen replenishment process of the present invention;
FIG. 6 is a block diagram of the components of the environmental control system of the present invention;
FIG. 7 is a block flow diagram of the gas concentration monitoring control system of the present invention;
FIG. 8 is a block flow diagram of the gas ambient temperature control system of the present invention;
FIG. 9 is a block flow diagram of the gas environment humidity control system of the present invention;
FIG. 10 is an interface for operation of the automatic fluid infusion control system of the present invention;
fig. 11 is an interface for operation of the gas concentration monitoring control system of the present invention.
In the figure: 1-console, 2-exhaust device, 3-liquid nitrogen supply pipeline, 4-liquid nitrogen supply tank, 5-temperature and humidity control device, 6-gas concentration detection module, 7-liquid nitrogen container, 8-low temperature electromagnetic valve and 9-exhaust valve.
Detailed Description
The present invention will be further described with reference to the accompanying drawings, which are illustrative of the present invention and are not to be construed as limiting the invention. In the description of the present application, it should be understood that the terms "upper", "lower", and the like refer to the orientation or positional relationship shown in the drawings, which are only used for convenience and simplicity of description, and do not indicate or imply that the device or component referred to must have a particular orientation, and therefore, should not be taken as limiting the scope of the present application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a control system of an intelligent biological sample repository, which comprises a biological sample repository, an automatic liquid nitrogen replenishing system and an environment control system, and is characterized in that: the biological sample storage warehouse comprises a control console 1, an exhaust device 2, a liquid nitrogen supply pipeline 3, a liquid nitrogen supply tank 4, a temperature and humidity control device 5, a gas concentration detection module 6, a liquid nitrogen container 7, a low-temperature electromagnetic valve 8 and an exhaust valve 9; as shown in fig. 1 and 2, the upper end of the liquid nitrogen container 7 is connected with a cryogenic solenoid valve 8, and the cryogenic solenoid valve 8 is connected with a branch pipe of the liquid nitrogen supply pipe 3 through a cryogenic metal hose. The branch pipeline of the liquid nitrogen supply pipeline 3 is welded with a matched exhaust valve 9, and the liquid nitrogen supply pipeline 3 is connected with a liquid nitrogen supply tank 4.
As shown in fig. 3, the automatic liquid supplementing system for liquid nitrogen comprises a core chip module, a liquid level acquisition module, a power supply module, a display module, a key module and an electromagnetic valve driving module; the liquid level acquisition module comprises a capacitance type liquid level sensor, an analog-digital converter and a singlechip.
As shown in fig. 6, the environment control system includes a core chip module, a programmable temperature control module, a power module, a display module, a key module, an exhaust fan driving module, and a gas concentration detection device 6; the exhaust fan driving module comprises an exhaust device 2 and a driving motor; the gas concentration detection module 6 comprises a gas concentration sensor, an alarm lamp and an alarm.
The console 1 is responsible for the management of the biological sample repository control system, the monitoring of the condition of the biological sample repository, and the easy analysis and testing.
As shown in fig. 1, the two exhaust devices 2 are respectively disposed on two walls near the outdoor space, so as to facilitate air exchange with the outside and reduce the concentration of nitrogen in the indoor air.
Preferably, two exhaust apparatus 2 shown all set up on the wall of the extension line department of the passageway that supplies people to walk, exhaust apparatus 2 and gas concentration sensor carry out the linkage in the control, and the purpose is when nitrogen gas concentration is too high, automatic opening exhaust apparatus 2, and the air of passageway department can be preferred exchanges with outside air, reduces the possibility of the unexpected injury that the people arouses because of oxygen concentration is not enough.
As shown in fig. 1, the number of the gas concentration detection modules 6 is three, and the three gas concentration detection modules are respectively arranged at the centers of the three walls. At a small biological sample repository of 40-60m2, three gas concentration sensors can just cover the entire room, effectively monitoring the concentration of nitrogen and oxygen.
Preferably, the low-temperature electromagnetic valve 8 is of a normally closed type, and is interlocked with a built-in liquid level meter of the liquid nitrogen container 7 in terms of control. The liquid level of the liquid nitrogen in the liquid nitrogen container 7 is usually 80% of the maximum volume, the liquid level at this time is set to be a standard liquid level and recorded in the control system, when the liquid level of the liquid nitrogen is lower than 80% or in an empty container state, the low-temperature electromagnetic valve 8 works to supply the liquid nitrogen to the inside of the container, and after the required amount of the liquid nitrogen is added to the inside of the liquid nitrogen container 7, the low-temperature electromagnetic valve 8 can be restored to an initial closed state. When the liquid nitrogen level in the liquid nitrogen container 7 is reduced to be lower than the preset value along with the time, the low-temperature electromagnetic valve 8 works again, and the operation is repeated in a circulating mode. If the automatic control of the low-temperature electromagnetic valve 8 fails, an operator can manually operate the valve to complete the opening and closing of the low-temperature electromagnetic valve 8.
Preferably, as shown in fig. 1, the temperature and humidity control devices 5 are four in number and are respectively placed at four corners of the wall. The aim is to regulate the temperature and humidity inside the biological sample storage warehouse and also to some extent to assist the air exchange of the exhaust device.
The present application also includes a control method of a control system for an intelligent biological sample repository, characterized in that: it comprises the following steps:
s1: starting an intelligent biological sample repository control system;
s2: starting an automatic liquid nitrogen supplementing system;
s3: starting an environment control system;
the working steps of the automatic liquid supplementing system for the liquid nitrogen comprise:
s1: setting the liquid level height as a preset value according to the requirement, wherein 80% of the preset value is also a safety value for freezing and storing the biological sample;
s2: liquid level signals are collected on the liquid nitrogen container 7 through the liquid level collecting module, the liquid level of liquid nitrogen is detected by the capacitive liquid level sensor in the process, the liquid level is measured according to the change of capacitance between the parallel polar plates, and the calculation formula of the capacitance C is as follows:
in the formula: w is the width of the electrode plate; h is the height of the electrode plate; h is x Is the liquid height between the electrode plates; d is the electrode plate spacing; epsilon x Is the relative dielectric constant of the liquid medium between the electrode plates; epsilon 0 Is the relative dielectric constant of the gaseous medium between the electrode plates.
The measured capacitance, the liquid level height and the preset dielectric constant are related, and the signal transmitter can obtain a calculation formula of the liquid level height of the liquid nitrogen as follows:
in the formula: epsilon Preparation of Is the relative dielectric constant of the liquid medium between the electrode plates.
And finally, converting the liquid level height of the liquid nitrogen into a volume value of the liquid nitrogen, and outputting the volume value to a display module for real-time display. The conversion formula of the liquid level height of the liquid nitrogen and the volume value of the liquid nitrogen is as follows:
in the formula: r is the radius of the inner wall of the liquid nitrogen container 7.
Then, switching judgment and switching time judgment are carried out on the normally closed low-temperature electromagnetic valve 8 according to the converted liquid level data;
s3: when the liquid level detection module detects that the liquid level of the liquid nitrogen in the liquid nitrogen container 7 is lower than 80% of a preset value, low-level alarming is carried out, and at the moment, a pin on the chip outputs a 3.3V high-level signal, so that a low-temperature electromagnetic valve 8 corresponding to the liquid nitrogen container 7 is opened, and the liquid nitrogen starts to be filled;
s4: along with the continuous filling of the liquid nitrogen, the liquid level of the liquid nitrogen in the liquid nitrogen container 7 starts to rise, and when the liquid level detection module detects that the liquid level of the liquid nitrogen container 7 for liquid supplement is greater than or equal to 95% of a preset value, a pin corresponding to the low-temperature electromagnetic valve 8 outputs 0V low level and closes the low-temperature electromagnetic valve 8;
preferably, as shown in fig. 10, in the automatic liquid nitrogen replenishment process, when the liquid level of the liquid nitrogen is lower than 80% of the preset value in the liquid level detection mode, a low liquid level alarm is performed, at this time, after receiving an alarm signal, an operator clicks an option of "automatic control" to enter an automatic replenishment mode, and in the automatic replenishment mode, the operator can manually click "turn on the number x low-temperature electromagnetic valve 8" and "turn off the number x low-temperature electromagnetic valve 8" to perform liquid replenishment; and the 'manual control' can be clicked to enter a pure manual mode, an operator manually controls the switch of the low-temperature electromagnetic valve 8 to observe whether the liquid level of the liquid nitrogen is close to a preset value, and then closes the low-temperature electromagnetic valve 8.
Preferably, as shown in fig. 5, the method for circularly supplementing liquid nitrogen can effectively alleviate the problem of the virtual high liquid level of the liquid nitrogen, and the method comprises the following specific steps: when the liquid nitrogen level data sensed by the capacitive sensor meets the preset value of more than 95%, the chip pin outputs a low level signal of 0V, so that the low-temperature electromagnetic valve 8 is in a continuously closed state, and the liquid nitrogen level signal is continuously acquired. After the liquid nitrogen supply is stopped, when the liquid nitrogen level data collected by the capacitance sensor is less than 80% of the preset value in a short time, the pin outputs a high level signal of 3.3V, the low-temperature electromagnetic valve 8 is opened again, and when the circulating liquid supply is stopped, the liquid nitrogen supplement is finished.
The working steps of the environment control system comprise gas concentration control, environment temperature control and environment humidity control:
as shown in fig. 7 and 11, for the working steps of the gas concentration control:
s1: setting the initial value of the oxygen concentration to be 21% and the initial value of the nitrogen to be 78%, presetting the oxygen concentration corresponding to each exhaust grade, and dividing the wind speed into 3 grades of low, medium and strong exhaust;
s2: acquiring an oxygen concentration signal through a gas concentration detection module 6;
s3: converting the acquired oxygen concentration signal into concentration data, and displaying the concentration data through a display module;
s4: starting low-grade exhaust when the detected oxygen concentration in the liquid nitrogen reservoir is more than 20% and less than or equal to 21%; when the oxygen concentration is more than 19% and less than or equal to 20%, starting middle-grade exhaust, and starting an alarm lamp and an alarm; when the oxygen concentration is less than or equal to 19 percent, strong-grade exhaust is started, and an alarm lamp and an alarm are turned on, so that the aim of reducing the nitrogen concentration in the air is fulfilled, and the multi-grade exhaust control also realizes energy conservation to a certain extent; the alarm lamp and the alarm are used for reminding working personnel in the biological sample storage warehouse to rapidly leave when the nitrogen concentration in the biological sample storage warehouse is too high;
s5: after exhausting for 20min, automatically closing the exhaust device 2, the alarm lamp and the alarm;
preferably, an audible and visual alarm device is additionally arranged at a place where personnel can observe conveniently in the biological sample storage library, wherein the audible and visual alarm device is the alarm lamp and the alarm, and the purpose is to remind the personnel in the biological sample storage library to leave rapidly when the nitrogen concentration in the biological sample storage library is too high. Staff can observe the oxygen concentration content and the nitrogen concentration content in the biological sample storage warehouse through a control system operation interface on a computer, and then whether the exhaust device 2 is started or not is checked through a sightseeing window on the gate of the biological sample storage warehouse, and if linkage failure occurs, the exhaust device 2 can be forcibly started.
As shown in fig. 8, for the working steps of the ambient temperature control:
s1: setting the initial environment temperature to be 15-28 ℃;
s2: detecting the ambient temperature;
s3: displaying the detection temperature, and processing:
when the ambient temperature is more than or equal to 28 ℃, the refrigeration mode of the air conditioner is started to reduce the ambient temperature to the range of the initial value; when the ambient temperature is less than or equal to 15 ℃, the heating mode of the air conditioner is started to increase the ambient temperature to the range of the initial value; when the ambient temperature reaches the range of the initial value, the air conditioner is in a low-power constant-temperature mode;
as shown in fig. 9, the working steps for controlling the ambient humidity are as follows:
s1: setting the initial environment humidity to be less than or equal to 40 percent;
s2: detecting the environmental humidity;
s3: displaying the detection temperature, and processing:
when the ambient humidity is more than 40%, the dehumidification function is started; and when the ambient humidity is less than or equal to 40%, the dehumidification function is closed.
The above is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily make changes or variations within the technical scope of the present invention disclosed, and such changes or variations should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. The utility model provides a control system of intelligence biological sample repository, includes biological sample repository, the automatic fluid infusion system of liquid nitrogen and environmental control system, its characterized in that: the biological sample storage warehouse comprises a control console (1), an exhaust device (2), a liquid nitrogen supply pipeline (3), a liquid nitrogen supply tank (4), a temperature and humidity control device (5), a gas concentration detection module (6), a liquid nitrogen container (7), a low-temperature electromagnetic valve (8) and an exhaust valve (9); the upper end of the liquid nitrogen container (7) is connected with a low-temperature electromagnetic valve (8), and the low-temperature electromagnetic valve (8) is connected with a branch pipeline of the liquid nitrogen supply pipeline (3) through a low-temperature metal hose; a branch pipeline of the liquid nitrogen supply pipeline (3) is welded with a matched exhaust valve (9), and the liquid nitrogen supply pipeline (3) is connected with a liquid nitrogen supply tank (4);
the liquid nitrogen automatic liquid supplementing system comprises a core chip module, a liquid level acquisition module, a power supply module, a display module, a key module and an electromagnetic valve driving module; the liquid level acquisition module comprises a capacitive liquid level sensor, an analog-to-digital converter and a singlechip;
the environment control system comprises a core chip module, a programmable temperature control module, a power supply module, a display module, a key module, an exhaust fan driving module and a gas concentration detection device (6); the exhaust fan driving module comprises an exhaust device (2) and a driving motor; the gas concentration detection module (6) comprises a gas concentration sensor, an alarm lamp and an alarm.
2. The control system of an intelligent biological sample repository of claim 1, wherein: the two exhaust devices (2) are respectively arranged on two walls close to the outside.
3. The control system of an intelligent biological sample repository of claim 1, wherein: the number of the gas concentration detection modules (6) is three, and the gas concentration detection modules are respectively arranged at the centers of the three walls.
4. The control system of an intelligent biological sample repository of claim 1, wherein: the four temperature and humidity control devices (5) are respectively arranged at four corners close to the wall.
5. The method of claim 1, wherein the method further comprises: it comprises the following steps:
s1: starting an intelligent biological sample repository control system;
s2: starting an automatic liquid supplementing system of liquid nitrogen;
s3: the environmental control system is started.
6. The method of claim 5, wherein the method further comprises: the working steps of the automatic liquid supplementing system for the liquid nitrogen comprise:
s1: setting the liquid level height as required, namely obtaining a preset value;
s2: a liquid level signal is acquired from a liquid nitrogen container (7) through a liquid level acquisition module, and the signal is subjected to analog-to-digital conversion and displayed through a liquid level display module;
s3: when the liquid level detection module detects that the liquid level of the liquid nitrogen in the liquid nitrogen container (7) is lower than 80% of a preset value, low-level alarm is carried out, and a low-temperature electromagnetic valve (8) corresponding to the liquid nitrogen container (7) is opened;
s4: when the liquid level detection module detects that the liquid level of the liquid nitrogen container (7) for liquid supplement is greater than or equal to 95% of the preset value, the low-temperature electromagnetic valve (8) corresponding to the liquid nitrogen container (7) is closed.
7. The method of claim 5, wherein the method further comprises: the working steps of the environment control system comprise gas concentration control, environment temperature control and environment humidity control:
working steps for gas concentration control:
s1: setting an initial value of the oxygen concentration to be 21% and an initial value of the nitrogen to be 78%, and presetting oxygen concentrations corresponding to each exhaust grade;
s2: acquiring an oxygen concentration signal through a gas concentration detection module (6);
s3: converting the acquired oxygen concentration signal into concentration data, and displaying the concentration data through a display module;
s4: starting low-grade exhaust when the detected oxygen concentration in the liquid nitrogen reservoir is more than 20% and less than or equal to 21%; when the oxygen concentration is more than 19% and less than or equal to 20%, starting middle-grade exhaust, and starting an alarm lamp and an alarm; when the oxygen concentration is less than or equal to 19%, strong-grade exhaust is started, and an alarm lamp and an alarm are turned on;
s5: after exhausting for 20min, automatically closing the exhaust device (2), the alarm lamp and the alarm;
working steps for ambient temperature control:
s1: setting the initial environment temperature to be 15-28 ℃;
s2: detecting the ambient temperature;
s3: displaying the detection temperature, and processing:
when the ambient temperature is more than or equal to 28 ℃, the refrigeration mode of the air conditioner is started to reduce the ambient temperature to the range of the initial value; when the ambient temperature is less than or equal to 15 ℃, the heating mode of the air conditioner is started to increase the ambient temperature to the range of the initial value; when the ambient temperature reaches the range of the initial value, the air conditioner is in a low-power constant-temperature mode;
the working steps of controlling the environmental humidity are as follows:
s1: setting the initial environment humidity to be less than or equal to 40%;
s2: detecting the environmental humidity;
s3: displaying the detection temperature, and processing:
when the ambient humidity is more than 40%, the dehumidification function is started; and when the ambient humidity is less than or equal to 40%, the dehumidification function is closed.
8. The method of claim 5, wherein the method further comprises: the liquid level acquisition module of the automatic liquid supplementing system for the liquid nitrogen detects the liquid level of the liquid nitrogen by using the capacitive liquid level sensor, and the liquid level is measured according to the change of capacitance between parallel polar plates, wherein the calculation formula of the capacitance C is as follows:
in the formula: w is the width of the electrode plate; h is the height of the electrode plate; h is x Is the liquid height between the electrode plates; d is the electrode plate spacing; epsilon x Is the relative dielectric constant of the liquid medium between the electrode plates; epsilon 0 Is the relative dielectric constant of the gas medium between the electrode plates;
according to the relation among the measured capacitance, the liquid level height and the preset dielectric constant, the signal transmitter can obtain a calculation formula of the liquid level height of the liquid nitrogen as follows:
in the formula: epsilon Preparing The relative dielectric constant of the liquid medium between the electrode plates is preset;
and finally, converting the liquid level height of the liquid nitrogen into a volume value of the liquid nitrogen, and outputting the volume value to a display module for real-time display, wherein the conversion formula of the liquid level height of the liquid nitrogen and the volume value of the liquid nitrogen is as follows:
in the formula: r is the radius of the inner wall of the liquid nitrogen container (7).
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Application publication date: 20220902 |