CN207020543U - A kind of gel column pipe holder - Google Patents
A kind of gel column pipe holder Download PDFInfo
- Publication number
- CN207020543U CN207020543U CN201720500166.9U CN201720500166U CN207020543U CN 207020543 U CN207020543 U CN 207020543U CN 201720500166 U CN201720500166 U CN 201720500166U CN 207020543 U CN207020543 U CN 207020543U
- Authority
- CN
- China
- Prior art keywords
- gel column
- sample solution
- column jecket
- pump
- pipe holder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000012488 sample solution Substances 0.000 claims abstract description 33
- 239000000523 sample Substances 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000012544 monitoring process Methods 0.000 abstract description 3
- 230000004888 barrier function Effects 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 238000001914 filtration Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000001641 gel filtration chromatography Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
It the utility model is related to a kind of gel column pipe holder, including the upper bearing plate positioned at top and the lower support plate positioned at bottom, upper bearing plate is supported in above lower support plate by leg, one or more gel column jecket putting holes are provided with upper bearing plate, and it is correspondingly provided with indicator lamp beside each gel column jecket putting hole, in lower support plate sample solution container rest area is provided with beside the position of face gel column jecket putting hole, the pump equal with gel column jecket putting hole quantity is additionally provided with support, each pump extends outward level sensor, level sensor electrically connects with pump.Gel column pipe holder of the present utility model can carry out automatic loading, and control the liquid level in gel column jecket, monitoring sample solution crosses column condition in real time, when sample solution crosses excessively slow post speed or end of the sample, indicator lamp corresponding to the sample is bright, prompts experimenter to remove barriers or carry out next step operation in time.
Description
Technical field
Experiment equipment is the utility model is related to, more specifically it relates to a kind of gel column pipe holder.
Background technology
Gel filtration chromatography is the technology commonly used in laboratory.Generally comprise following steps:Gel resin is loaded into gel
In column jecket, gel column is formed, sample solution is flowed through gel column (that is, loading), molecules of interest is adsorbed solidifying by suction-operated
On glue post, molecules of interest is then eluted from gel column with eluent.During gel filtration chromatography, most time-consuming is loading step
Suddenly.One or two hour is generally spent, when loading system is very big, or even to spend several hours.In this course, it is necessary to not
Add sample solution with stopping, control the sample solution height in gel column jecket, and it also requires attention sample solution crosses post speed
Degree, serve as operating, it is necessary to take and handle accordingly when post speed is too slow.Therefore, gel filtration chromatography is an experiment wasted time and energy,
And when sample number is a lot, experimenter can not dig-in a sample and carry out monitoring filtering state
Therefore, it is necessary to which a kind of new gel column pipe holder carries out automatic loading and each sample of assistant experiment personnel control
Mistake column state.
Utility model content
In order to solve problem above, the utility model provides a kind of gel column pipe holder, and it is included positioned at the upper of top
Support plate and the lower support plate positioned at bottom, the upper bearing plate are supported in above the lower support plate by leg, it is described on
One or more gel column jecket putting holes are provided with support plate, and are accordingly set beside each gel column jecket putting hole
It is equipped with indicator lamp, being provided with sample solution container beside the position of gel column jecket putting hole described in face in the lower support plate puts
Area is put, the container equipped with sample solution is positioned on sample solution container rest area, is additionally provided with the support and is coagulated with described
Glue column jecket places the equal pump of hole number, and each pump extends outward level sensor, the level sensor with it is described
Pump electrically connects, and the probe of the level sensor can stretch into the gel column that will be positioned in corresponding gel column jecket putting hole
Guan Zhong, weight sensing unit is provided with the sample solution container rest area, the weight sensing unit detects the sample
Indicator lamp electrical connection when constant of the weight of sample solution container on product solution container rest area.
Gel column pipe holder of the present utility model can carry out automatic loading, and control the liquid level in gel column jecket, real
When monitoring sample solution cross column condition, when sample solution crosses excessively slow post speed or end of the sample, indicator lamp corresponding to the sample is bright,
Prompting experimenter removes barriers or carried out next step operation in time.
Further, the weight sensing unit includes Weight acquisition device, single-chip microcomputer and comparator with store function,
The output end of the Weight acquisition device is connected with the input of the single-chip microcomputer, and the single-chip microcomputer has the first output end and second
Output end, the first output end of the single-chip microcomputer are connected with an input of the comparator, and the second of the single-chip microcomputer is defeated
Go out end to be connected with another input of the comparator, the output end of the comparator electrically connects with corresponding indicator lamp.
In another embodiment, the acquisition interval of the Weight acquisition device is 1-2mi n.It can adjust as needed
The acquisition interval of Weight acquisition device.
In another embodiment, the pump is arranged at the top of the upper bearing plate.With facilitate water inlet pipe, outlet pipe and
The setting of level sensor probe.
In another embodiment, buzzer, the buzzer and all comparators electricity are additionally provided with the support
Connection.Buzzer can prompt experimenter so that experimenter without staying in filtering area always by sight.
Brief description of the drawings
Fig. 1 is the schematic diagram of filtering support of the present utility model;
Fig. 2 is the annexation figure of weight collector, comparator and indicator lamp.
In accompanying drawing, the list of parts representated by each label is as follows:
1st, upper bearing plate, 2, lower support plate, 3, funnel putting hole, 4, sample solution container rest area, 5, indicator lamp, 6,
Pump, 7, water inlet pipe, 8, outlet pipe, 9, gel column jecket.
Embodiment
Principle of the present utility model and feature are described below in conjunction with accompanying drawing, example is served only for explaining this practicality
It is new, it is not intended to limit the scope of the utility model.
As illustrated in fig. 1 and 2, gel column pipe holder of the present utility model, it is characterised in that including the upper supporting positioned at top
Plate 1 and the lower support plate 2 positioned at bottom, the upper bearing plate 1 are supported in the top of the lower support plate 2 by leg, it is described on
One or more gel column jecket putting holes 3 are provided with support plate 1, and each side of gel column jecket putting hole 3 is corresponding
Ground is provided with indicator lamp 5, and it is molten beside the position of gel column jecket putting hole 3 described in face to be provided with sample in the lower support plate 2
Liquid container rest area 4, the container equipped with sample solution are positioned on sample solution container rest area 4, also set up on the support
There is the pump 6 equal with 3 quantity of the gel column jecket putting hole, each pump 6 extends outward level sensor, the water level
Sensor electrically connects with the pump 6, and the probe of the level sensor can stretch into and will be positioned over corresponding gel column jecket and put
Put in the gel column jecket in hole 3, weight sensing unit, the weight detecting are provided with the sample solution container rest area 4
Unit detects that the indicator lamp 5 is electrically connected when the weight of the sample solution container on the sample solution container rest area 4 is constant
Connect.
In chromatography process, sample solution container is positioned on sample solution container rest area 4, and gel column jecket 9 is put into gel
In column jecket putting hole 3, the water inlet pipe 7 of pump 6 extends the bottom of sample solution container, and outlet pipe 8 is extended in gel column jecket 9, water level
The probe of sensor extends detecting water level in water level gel column jecket 9, when water level is less than probe, the controlling pump 6 of level sensor 10
Sample solution in sample solution container is uploaded in gel column jecket 9 by work, pump 6, the weight saving of sample solution container, when
When water level is higher than probe, level sensor controlling pump 6 is stopped.Weight sensing unit detects sample solution at regular intervals
The weight change of container, when end of the sample or cross post speed it is excessively slow when, persistently higher than probe, pump 6 continues not work water level, weight
The adjacent weight that collects twice of amount collector is constant, and indicator lamp 5 is bright.
Further, the weight sensing unit includes Weight acquisition device, single-chip microcomputer and comparator with store function,
The output end of the Weight acquisition device is connected with the input of the single-chip microcomputer, and the single-chip microcomputer has the first output end and second
Output end, the first output end of the single-chip microcomputer are connected with an input of the comparator, and the second of the single-chip microcomputer is defeated
Go out end to be connected with another input of the comparator, the output end of the comparator electrically connects with corresponding indicator lamp 5.
In another embodiment, the acquisition interval of the Weight acquisition device is 1-2mi n.It can adjust as needed
The acquisition interval of Weight acquisition device.
In another embodiment, the pump 6 is arranged at the top of upper bearing plate 1.To facilitate water inlet pipe 7, water outlet
The setting of pipe 8 and level sensor probe.
In another embodiment, buzzer, the buzzer and all comparators electricity are additionally provided with the support
Connection.Buzzer can prompt experimenter so that experimenter without staying in filtering area always by sight.
Preferred embodiment of the present utility model is the foregoing is only, it is all in this practicality not to limit the utility model
Within new spirit and principle, any modification, equivalent substitution and improvements made etc., guarantor of the present utility model should be included in
Within the scope of shield.
Claims (5)
1. a kind of gel column pipe holder, it is characterised in that including the upper bearing plate (1) positioned at top and the lower support positioned at bottom
Plate (2), the upper bearing plate (1) are supported in above the lower support plate (2) by leg, are set on the upper bearing plate (1)
There are one or more gel column jecket putting holes (3), and be correspondingly provided with referring to beside each gel column jecket putting hole (3)
Show lamp (5), be provided with sample solution appearance on the lower support plate (2) beside the position of gel column jecket putting hole (3) described in face
Device rest area (4), the container equipped with sample solution are positioned on sample solution container rest area (4), also set up on the support
There is the pump (6) equal with gel column jecket putting hole (3) quantity, each pump (6) extends outward level sensor, institute
Level sensor (10) is stated to electrically connect with the pump (6), and the probe of the level sensor can stretch into will be positioned over it is corresponding
Gel column jecket putting hole (3) in gel column jecket in, be provided with weight detecting list in the sample solution container rest area (4)
Member, the weight sensing unit detect that the weight of the sample solution container on the sample solution container rest area (4) is constant
Shi Suoshu indicator lamps (5) electrically connect.
2. gel column pipe holder according to claim 1, the weight sensing unit includes Weight acquisition device, with storage
The single-chip microcomputer and comparator of function, the output end of the Weight acquisition device are connected with the input of the single-chip microcomputer, the monolithic
Machine has the first output end and the second output end, and the first output end of the single-chip microcomputer connects with an input of the comparator
Connect, the second output end of the single-chip microcomputer is connected with another input of the comparator, the output end of the comparator with
Corresponding indicator lamp (5) electrical connection.
3. gel column pipe holder according to claim 2, it is characterised in that the acquisition interval of the Weight acquisition device is 1-
2min。
4. gel column pipe holder according to claim 1, it is characterised in that the pump (6) is arranged at the upper bearing plate
(1) top.
5. the gel column pipe holder according to any one of claim 1-4, it is characterised in that be additionally provided with the support
Buzzer, the buzzer electrically connect with all comparators.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201720500166.9U CN207020543U (en) | 2017-05-05 | 2017-05-05 | A kind of gel column pipe holder |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201720500166.9U CN207020543U (en) | 2017-05-05 | 2017-05-05 | A kind of gel column pipe holder |
Publications (1)
Publication Number | Publication Date |
---|---|
CN207020543U true CN207020543U (en) | 2018-02-16 |
Family
ID=61475918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201720500166.9U Expired - Fee Related CN207020543U (en) | 2017-05-05 | 2017-05-05 | A kind of gel column pipe holder |
Country Status (1)
Country | Link |
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CN (1) | CN207020543U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110317714A (en) * | 2019-08-14 | 2019-10-11 | 北京市中医研究所 | The PCR microwell plate adapter of low temperature tape light |
-
2017
- 2017-05-05 CN CN201720500166.9U patent/CN207020543U/en not_active Expired - Fee Related
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110317714A (en) * | 2019-08-14 | 2019-10-11 | 北京市中医研究所 | The PCR microwell plate adapter of low temperature tape light |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180216 |
|
CF01 | Termination of patent right due to non-payment of annual fee |