CN114984293B

CN114984293B - Wound antibacterial healing-promoting dressing and preparation method thereof

Info

Publication number: CN114984293B
Application number: CN202210758005.5A
Authority: CN
Inventors: 高亮
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-06-29
Filing date: 2022-06-29
Publication date: 2023-10-17
Anticipated expiration: 2042-06-29
Also published as: CN114984293A

Abstract

The application discloses a wound antibacterial healing-promoting dressing and a preparation method thereof, belonging to the technical field of medical dressing processing, and comprising the following steps: s1, preparing medicinal powder; s2, preparing drug-loaded fibers; s3, preparing secretion absorbing fibers; s4, spinning; s5, spraying. The application breaks through the conventional dressing manufacturing method, blends the medicine-carrying part and the secretion absorbing part into different fibers, and adopts a warp-weft spinning mode to vertically and horizontally distribute the secretion absorbing part and the medicine-carrying antibacterial healing-promoting part, so that the prepared dressing has mechanical strength, can effectively avoid the negative influence of wound secretion on antibacterial healing-promoting, and can sterilize and heal wounds while sucking the secretion. Finally, spraying hydrogel with poly-N-vinyl caprolactam (PNVCL) and Graphene Oxide (GO) as matrix materials on one side of the blended gauze, mainly for leading out wound secretion.

Description

Wound antibacterial healing-promoting dressing and preparation method thereof

Technical Field

The application belongs to the technical field of medical dressing processing, and particularly relates to a wound antibacterial healing-promoting dressing and a preparation method thereof.

Background

Methods and means of wound treatment have undergone a number of changes over time. With the development of the age, people have come to use materials obtained from nature such as leaves, honey, spider web, bark, vinegar, wine, etc. for wound treatment. About 1600 years before the metric element, people soaked flax strips in oil and attached plasters, which were used as wound dressings. By 400 years before the male, hippocrates et al used honey mixed wine or vinegar to clean wounds and also used water or wine-boiled wool as a medical bandage, with the development of scientific research, the advent of antibacterial methods and antibiotics marked an important advance in modern medicine in the 19 th century; later in the 20 th century, modern wound dressings were born. Nowadays, the dressing can not only better cover the wound surface, but also promote the healing and recovery of the wound. The dressing mainly plays a role in absorbing secretion and healing wounds, but when the wound is healed, the released secretion can play a role in the healing, and even can cause the proliferation of mixed bacteria at the wound, so that the wound is healed all the time.

The application number 201610745185.8 discloses a wound antibacterial healing-promoting dressing, and the effective components of the dressing are paste comprising silk fibroin raw materials, bioactive glass raw materials, chitosan raw materials and polyvinyl alcohol raw materials which are uniformly mixed. The method for preparing the dressing comprises the steps of firstly uniformly mixing a silk fibroin solution and bioactive glass powder, then adding a chitosan solution and a polyvinyl alcohol solution, stirring to form a mixed solution, repeatedly freezing and thawing to obtain a wound dressing material difficult to heal, and controlling the water vapor permeability, the water absorption, the water retention and the antibacterial property of the obtained dressing by adjusting the proportion among the silk fibroin solution, the chitosan solution, the polyvinyl alcohol solution and the bioactive glass serving as raw materials to enable the dressing to meet the requirements of promoting wound healing; the preparation method has the advantages of mild reaction conditions, simple process and low raw material cost, and is suitable for industrial scale-up production. Although the dressing obtained by the application method has the water vapor permeability, water absorption, water retention and antibacterial performance, and can meet the requirements of wound healing, the negative influence caused by secretion at the wound cannot be avoided.

Disclosure of Invention

The application aims at solving the existing problems and provides a wound antibacterial healing-promoting dressing and a preparation method thereof.

The application is realized by the following technical scheme:

a preparation method of a wound antibacterial healing-promoting dressing comprises the following steps:

s1, preparing medicinal powder:

s101, placing natural indigo, borax and gentian together in a bead mill according to a weight ratio of 1:7-10:10-12, adding liquid nitrogen for grinding, and taking out powder A for standby after finishing;

s102, placing the powder A obtained in the step S101 into a corona discharge instrument for corona treatment, and then placing into a superfine pulverizer for pulverization treatment to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s201, uniformly stirring and mixing a polyvinyl alcohol solution and a sodium alginate solution according to the weight ratio of 3-4:1 under the constant temperature condition to obtain a mixed solution A for standby;

s202, adding the medicinal powder obtained in the step S1 into the mixed solution A, and then placing the mixed solution A into a micro-jet high-pressure homogenizer for high-pressure homogenization treatment to obtain a mixed solution B for later use;

s203, spinning the mixed solution B obtained in the step S202 to obtain a drug-loaded fiber for later use;

s3, preparing secretion absorbing fibers:

adding poly-N-vinyl caprolactam (PNVCL) and Graphene Oxide (GO) into nylon melt spinning to prepare a secretion absorbing fiber for later use;

s4, spinning:

spinning the obtained medicine carrying fiber and secretion absorbing fiber in a warp-weft spinning mode to obtain blended gauze for standby;

s5, spraying:

one side of the blended gauze is sprayed with hydrogel taking poly-N-vinyl caprolactam (PNVCL) and Graphene Oxide (GO) as matrix materials.

Further, the polishing rate is controlled to 300 to 400rpm during the polishing treatment in step S101, and the polishing treatment time is controlled to 30 to 40rpm.

Further, the operating voltage is controlled to be 10-12 kV during the corona treatment in the step S102, and the time of the corona treatment is 30-40S.

Further, the constant temperature in step S201 is 60 to 70 ℃.

Further, the working pressure is controlled to be 70-80 MPa during the high-pressure homogenizing treatment in the step S202, and the homogenizing treatment time is 40-60S.

Compared with the prior art, the application has the following advantages:

1. the application provides a preparation method of wound antibacterial and healing promoting dressing, which is characterized in that natural indigo, borax and gentian are specially treated to prepare medicinal powder, and then the medicinal powder is added into the preparation of medicine-carrying fibers, so that the medicinal powder can be well fused with the fibers and can be slowly released in the use of the dressing, and the antibacterial and healing promoting effect is achieved.

2. According to the application, poly-N-vinyl caprolactam (PNVCL) and Graphene Oxide (GO) are added into nylon spinning to prepare the secretion absorbing fiber, and the fiber can well absorb wound secretion in the use of the dressing.

3. The application breaks through the conventional dressing manufacturing method, blends the medicine-carrying part and the secretion absorbing part into different fibers, and adopts a warp-weft spinning mode to vertically and horizontally distribute the secretion absorbing part and the medicine-carrying antibacterial healing-promoting part, so that the prepared dressing has mechanical strength, can effectively avoid the negative influence of wound secretion on antibacterial healing-promoting, and can sterilize and heal wounds while sucking the secretion. Finally, spraying hydrogel with poly-N-vinyl caprolactam (PNVCL) and Graphene Oxide (GO) as matrix materials on one side of the blended gauze, mainly for leading out wound secretion.

Detailed Description

Example 1:

s1, preparing medicinal powder:

s101, placing indigo naturalis, borax and gentian together in a bead mill according to a weight ratio of 1:7:10, adding liquid nitrogen for grinding, controlling the grinding speed to be 300rpm, and taking out powder A for standby after grinding treatment is performed at 30 rpm;

s102, placing the powder A obtained in the step S101 into a corona discharge instrument for corona treatment, controlling the working voltage to be 10kV, controlling the corona treatment time to be 30S, and then placing the powder A into a superfine pulverizer for pulverizing treatment to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s201, uniformly stirring and mixing a polyvinyl alcohol solution and a sodium alginate solution according to a weight ratio of 3:1 at a constant temperature of 60 ℃ to obtain a mixed solution A for standby;

s202, adding the medicinal powder obtained in the step S1 into the mixed solution A, then placing the mixed solution A into a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 70MPa, and homogenizing for 40 seconds to obtain a mixed solution B for later use;

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Example 2:

s1, preparing medicinal powder:

s101, placing indigo naturalis, borax and gentian together in a bead mill according to a weight ratio of 1:8.5:11, adding liquid nitrogen for grinding, controlling the grinding speed to be 350rpm, and taking out powder A for standby after grinding treatment is carried out at 35 rpm;

s102, placing the powder A obtained in the step S101 into a corona discharge instrument for corona treatment, controlling the working voltage to be 11kV, controlling the corona treatment time to be 35S, and then placing the powder A into a superfine pulverizer for pulverizing treatment to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s201, uniformly stirring and mixing a polyvinyl alcohol solution and a sodium alginate solution according to the weight ratio of 3.5:1 at the constant temperature of 65 ℃ to obtain a mixed solution A for standby;

s202, adding the medicinal powder obtained in the step S1 into the mixed solution A, then placing the mixed solution A into a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 75MPa, and homogenizing for 50S to obtain a mixed solution B for later use;

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Example 3:

s1, preparing medicinal powder:

s101, placing indigo naturalis, borax and gentian together in a bead mill according to a weight ratio of 1:10:12, adding liquid nitrogen for grinding, controlling the grinding speed to be 400rpm, and taking out powder A for standby after grinding treatment at 40 rpm;

s102, placing the powder A obtained in the step S101 into a corona discharge instrument for corona treatment, controlling the working voltage to be 12kV, controlling the corona treatment time to be 40S, and then placing the powder A into a superfine pulverizer for pulverizing treatment to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s201, uniformly stirring and mixing a polyvinyl alcohol solution and a sodium alginate solution according to a weight ratio of 4:1 at a constant temperature of 70 ℃ to obtain a mixed solution A for standby;

s202, adding the medicinal powder obtained in the step S1 into the mixed solution A, then placing the mixed solution A into a micro-jet high-pressure homogenizer for high-pressure homogenization treatment, controlling the working pressure to be 80MPa, and homogenizing for 60 seconds to obtain a mixed solution B for later use;

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Comparative example 1:

s1, preparing medicinal powder:

uniformly mixing indigo naturalis, borax and gentian according to a weight ratio of 1:8.5:11, placing the mixture in a corona discharge instrument for corona treatment, controlling the working voltage to be 11kV, controlling the time of the corona treatment to be 35s, and then placing the mixture in an ultrafine pulverizer for pulverizing to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Comparative example 2:

s1, preparing medicinal powder:

s102, placing the powder A obtained in the step S101 into a superfine pulverizer for pulverizing treatment to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Comparative example 3:

s1, preparing medicinal powder:

s102, placing the powder A obtained in the step S101 into a corona discharge instrument for corona treatment, controlling the working voltage to be 11kV, and carrying out corona treatment for 35S to obtain medicinal powder;

s2, preparing drug-loaded fibers:

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Comparative example 4:

s1, preparing medicinal powder:

s2, preparing drug-loaded fibers:

s202, adding the medicinal powder obtained in the step S1 into the mixed solution A, and uniformly mixing to obtain a mixed solution B for standby;

s3, preparing secretion absorbing fibers:

s4, spinning:

s5, spraying:

Comparative example 5:

s1, preparing medicine carrying fibers:

s101, uniformly stirring and mixing a polyvinyl alcohol solution and a sodium alginate solution according to a weight ratio of 3.5:1 at a constant temperature of 65 ℃ to obtain a mixed solution A for standby;

s102, carrying out spinning treatment on the mixed solution A obtained in the step S201 to obtain a medicine-carrying fiber for standby;

s2, preparing secretion absorbing fibers:

s3, spinning:

s4, spraying:

Comparative example 6:

s1, preparing medicinal powder:

s2, preparing drug-loaded fibers:

s4, spinning:

spinning the obtained medicine carrying fiber in a warp-weft spinning mode to obtain gauze for standby;

s5, spraying:

one side of the gauze is sprayed with hydrogel taking poly-N-vinyl caprolactam (PNVCL) and Graphene Oxide (GO) as matrix materials.

Comparative example 7:

s1, preparing secretion absorbing fibers:

s2, spinning:

spinning the secretion absorbing fiber in a warp and weft spinning mode to obtain gauze for standby;

s3, spraying:

Control group

The application number is: 201610745185.8A wound antibacterial and healing promoting dressing and its preparation method are disclosed.

In order to compare the technical effects of the present application, dressing materials were prepared correspondingly by the methods of the above example 2, comparative examples 1 to 7 and control group, respectively, and then an in vitro antibacterial effect test and a rat operation wound healing test were performed.

1. In vitro antibacterial effect test

The common wound strain, namely staphylococcus aureus, escherichia coli, streptococcus pyogenes and pseudomonas aeruginosa, is adopted as an experimental strain. 0.5ml of each bacterial liquid (concentration 10) ⁵ ～10 ⁶ CFU/ml) was added dropwise to the blank (medical dressing without any antibacterial component added), example 2, comparative examples 1 to 7 and dressing samples of the control group, the sterilized cover sheets were held by sterilized forceps, covered on the samples, respectively, spread flat and bubble-free, the bacteria were allowed to contact the samples uniformly, placed in a sterilized plate, cultured for 24 hours at (37.+ -. 1) C and relative humidity > 90%, and then taken out, 20ml of washing solution was added, the samples and coverslips were repeatedly washed, and after sufficient shaking, the washing solution was inoculated into nutrient agar medium, and after culturing for 24 hours at (37.+ -. 1) C, viable bacteria were counted. The antibacterial rates of example 2, comparative examples 1 to 7, and the control group were calculated from the colony count of the blank group. The experimental results are shown in table 1.

Table 1 in vitro bacteriostatic effects (bacteriostatic Rate)%)

	Staphylococcus aureus	Coli bacterium	Streptococcus pyogenes	Pseudomonas aeruginosa
					Example 2	100	99.3	100	98.5
Comparative example 1	98.6	98.2	97.3	98.0
					Comparative example 2	97.3	96.5	95.6	96.3
Comparative example 3	99.3	96.2	98.1	97.2
					Comparative example 4	96.3	95.5	97.2	94.0
Comparative example 5	83.3	76.6	82.0	68.3
					Comparative example 6	89.6	87.3	85.2	81.0
Comparative example 7	63.6	65.2	64.2	70.0
					Control group	85.3	85.3	84.3	86.0

As can be seen from the above table 1,

the dressing provided by the application has a good in-vitro antibacterial effect, the antibacterial rate of staphylococcus aureus, escherichia coli, streptococcus pyogenes and pseudomonas aeruginosa can reach more than 98%, the antibacterial effect is obvious, and the dressing is obviously superior to a control group, namely, the dressing prepared by the method provided by the application can effectively propagate microorganisms on wound parts all the time, and the incidence rate of wound infection is reduced.

2. Rat surgical wound healing test

90 adult healthy SD rats were anesthetized with 30mg/kg volume fraction 10% chloral hydrate by intraperitoneal injection, shaved on the back, and exposed skin. The back chest section is taken as the center, and the whole layer of skin is cut off to deep fascia according to the size of 2cm multiplied by 2cm, so that a square wound surface is formed. The post-operative animals were randomly divided into 9 groups, each corresponding to example 2, comparative examples 1 to 7, and control group, 10, and dressing corresponding to the group was used for dressing. Dressing corresponding to the group was changed daily after the operation, wound appearance was observed and recorded, wound length and width were measured at 4,7,11,14,21 th day after the operation, wound area and wound healing rate were calculated, wound healing rate= (initial wound area-wound area measured at n-th day)/initial wound area×100%, and experimental results are shown in table 2.

Table 2 healing promoting effect of each set of dressing on rat surgical wounds

The second 2 shows that the dressing prepared by the method has obvious healing promoting effect on rat operation wounds, tender and effective tissue adhesion and wound crusting prevention, the secretion absorption and drug release antibacterial effect of the dressing are separated, mutual interference is avoided, the healing rate of wounds after two weeks treatment can reach more than 98, and the wound is clean and safe and has no infection through observation.

Claims

1. The preparation method of the wound antibacterial healing-promoting dressing is characterized by comprising the following steps of:

s1, preparing medicinal powder:

s2, preparing drug-loaded fibers:

s3, preparing secretion absorbing fibers:

adding poly-N-vinyl caprolactam and graphene oxide into the melt spinning of nylon to prepare a secretion absorbing fiber for later use;

s4, spinning:

s5, spraying:

one side of the blended gauze is sprayed with hydrogel taking poly-N-vinyl caprolactam and graphene oxide as matrix materials.

2. The method for preparing a wound antibacterial healing-promoting dressing according to claim 1, wherein the grinding speed is controlled to be 300-400 rpm during the grinding treatment in the step S101, and the grinding treatment time is 30-40 rpm.

3. The method for preparing a wound antibacterial healing-promoting dressing according to claim 1, wherein the operating voltage is controlled to be 10-12 kV during the corona treatment in the step S102, and the time of the corona treatment is 30-40S.

4. The method for preparing a wound antibacterial healing-promoting dressing according to claim 1, wherein the constant temperature in the step S201 is 60-70 ℃.

5. The method for preparing a wound antibacterial healing-promoting dressing according to claim 1, wherein the working pressure is controlled to be 70-80 MPa during the high-pressure homogenization treatment in the step S202, and the homogenization treatment time is 40-60S.

6. A wound bacteriostatic healing-promoting dressing, characterized by being prepared by the method of any one of the above claims 1-5.