CN114981402A - 用于可在生物反应器中规模化制造治疗性细胞的系统和方法 - Google Patents

用于可在生物反应器中规模化制造治疗性细胞的系统和方法 Download PDF

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CN114981402A
CN114981402A CN202180009616.3A CN202180009616A CN114981402A CN 114981402 A CN114981402 A CN 114981402A CN 202180009616 A CN202180009616 A CN 202180009616A CN 114981402 A CN114981402 A CN 114981402A
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bioreactor
scale
edr
mixer
suspension
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Pending
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CN202180009616.3A
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Chinese (zh)
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赞容·布莱恩·李
布丽安娜·莎琳·鲍里斯
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PBS Biotech Inc
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PBS Biotech Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Analytical Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Rheumatology (AREA)
  • Mechanical Engineering (AREA)
  • Computer Hardware Design (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202180009616.3A 2020-01-27 2021-01-27 用于可在生物反应器中规模化制造治疗性细胞的系统和方法 Pending CN114981402A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062966441P 2020-01-27 2020-01-27
US62/966,441 2020-01-27
PCT/US2021/015256 WO2021154832A1 (en) 2020-01-27 2021-01-27 Systems and methods for scalable manufacturing of therapeutic cells in bioreactors

Publications (1)

Publication Number Publication Date
CN114981402A true CN114981402A (zh) 2022-08-30

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CN202180009616.3A Pending CN114981402A (zh) 2020-01-27 2021-01-27 用于可在生物反应器中规模化制造治疗性细胞的系统和方法

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Country Link
US (1) US20210230532A1 (cg-RX-API-DMAC7.html)
EP (1) EP4097216A1 (cg-RX-API-DMAC7.html)
JP (1) JP7727664B2 (cg-RX-API-DMAC7.html)
CN (1) CN114981402A (cg-RX-API-DMAC7.html)
IL (1) IL294433A (cg-RX-API-DMAC7.html)
WO (1) WO2021154832A1 (cg-RX-API-DMAC7.html)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7393945B2 (ja) 2017-03-31 2023-12-07 テルモ ビーシーティー、インコーポレーテッド 細胞増殖
US20210002598A1 (en) * 2019-07-02 2021-01-07 Pbs Biotech, Inc. Methods of mixing and dispensing cells
US12522795B2 (en) * 2021-07-20 2026-01-13 The Texas A&M University System 3D rapid prototypable tunable peristalsis bioreactor
US20240101946A1 (en) * 2022-09-19 2024-03-28 Terumo Bct, Inc. Expanding Cells
US20260212091A1 (en) 2022-12-19 2026-07-23 Peter Satzer Scalable physical reactor representation
US20240309309A1 (en) * 2023-03-17 2024-09-19 Tata Consultancy Services Limited Systems and methods for optimizing a bioreactor for controlling growth of human stem cells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103153318A (zh) * 2010-09-02 2013-06-12 生命技术公司 用于细胞的生物反应器放大的细胞培养系统
US20160215257A1 (en) * 2015-01-16 2016-07-28 General Electric Company Pluripotent stem cell expansion and passage using a stirred tank bioreactor
US20190054432A1 (en) * 2016-07-18 2019-02-21 Cellmotions Inc. Low shear, low velocity differential, impeller having a progressively tapered hub volume with periods formed into a bottom surface, systems and methods for suspension cell culturing

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103153318A (zh) * 2010-09-02 2013-06-12 生命技术公司 用于细胞的生物反应器放大的细胞培养系统
US20160215257A1 (en) * 2015-01-16 2016-07-28 General Electric Company Pluripotent stem cell expansion and passage using a stirred tank bioreactor
US20190054432A1 (en) * 2016-07-18 2019-02-21 Cellmotions Inc. Low shear, low velocity differential, impeller having a progressively tapered hub volume with periods formed into a bottom surface, systems and methods for suspension cell culturing

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGÉLIQUE DELAFOSSE等: "Estimation of the turbulent kinetic energy dissipation rate from 2D-PIV measurements in a vessel stirred by an axial Mixel TTP impeller", CHEMICAL ENGINEERING SCIENCE, vol. 66, 14 January 2011 (2011-01-14), pages 1728 - 1737, XP028173649, DOI: 10.1016/j.ces.2011.01.011 *
CHRIS JOHNSON等: "Verification of energy dissipation rate scalability in pilot and production scale bioreactors using computational fluid dynamics", BIOTECHNOLOGY PROGRESS, vol. 30, 10 March 2014 (2014-03-10), pages 67 - 76 *
张建文;刘禹;夏建业;郭美锦;王泽建;庄英萍;: "机械搅拌生物反应器的CFD模拟及剪切力对红花细胞悬浮培养的影响", 华东理工大学学报(自然科学版), vol. 42, no. 04, 30 August 2016 (2016-08-30), pages 492 - 498 *
颜旭;张志强;顾承真;洪厚胜;: "适用于动物细胞培养的搅拌桨及其数值模拟", 现代化工, vol. 34, no. 10, 20 October 2014 (2014-10-20), pages 162 - 166 *

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EP4097216A1 (en) 2022-12-07
JP7727664B2 (ja) 2025-08-21
US20210230532A1 (en) 2021-07-29
JP2023517777A (ja) 2023-04-26
IL294433A (en) 2022-09-01
WO2021154832A1 (en) 2021-08-05

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