CN114965424A - SERS (surface enhanced Raman scattering) rapid analysis method for energy intake - Google Patents
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses a SERS rapid analysis method of energy intake, which comprises the following steps: (1) filling a pre-filled liquid; (2) collecting an SERS signal; (3) establishing a urine SERS database; (4) and (4) analyzing a main component based on the urine SERS database. According to the invention, by collecting urine Surface Enhanced Raman Spectroscopy (SERS) signals of people with different energy intakes and combining a principal component analysis algorithm, a relation between the SERS signals of urine and the energy intakes is established, namely, the energy intake information of the people to be detected can be obtained by collecting the SERS signals of urine of the people to be detected; meanwhile, the invention designs an integrated sol testing small tube, solves the problem of complicated operation caused by carrying reagents such as nano enhanced particle sol, nano particle aggregating agent and the like in the using process, and can complete the test without professional technicians and any large-scale instrument and equipment; the SERS method is simple to operate, low in cost, high in speed, portable in instrument and easy to popularize, and is very suitable for rapidly evaluating the energy intake on site.
Description
Technical Field
The invention relates to the field of analysis and test, in particular to a rapid SERS analysis method for energy intake.
Background
The aging mechanism of human beings has been a hot point of research. Over the past decades, aging mechanisms have been devoted to the study of energy intake. Excessive caloric intake can produce reactive oxygen species in the body, causing damage to DNA, phospholipids or proteins, accelerating the aging of the organism. While sustained low calorie intake, while maintaining the necessary nutrient intake, will have beneficial effects on the health and anti-aging of the body.
At present, the calculation of the energy intake is mainly evaluated by a meal retrospective method, substances which can provide energy in foods are carbohydrate, fat and protein, physiological energy values of each gram of the carbohydrate, the fat and the protein are respectively 3.99 kcal, 9.01 kcal and 3.99 kcal, and the energy intake of an individual can be roughly evaluated by calculating the mass of the carbohydrate, the fat and the protein in the ingested meal. Although the dietary review method can roughly evaluate the energy intake of an individual, the method is complex and time-consuming to operate, and a method for quickly and simply analyzing the energy intake is needed.
SERS (surface enhanced Raman Spectroscopy) is a molecular vibration and rotation spectrum based on a Raman scattering effect, can provide structural information of sample molecules under the condition of no damage, and performs composition and molecular structure analysis on a sample, so that qualitative analysis on the molecules is realized, the Raman spectrum intensity is in positive correlation with the number of scattered molecules, and semi-quantitative analysis on the molecules can be realized. The sol method is one of the testing methods of surface enhanced Raman spectroscopy, and the testing process of the sol method generally mixes three components of the nanoparticle aggregating agent, the nano enhanced particles and the substance to be tested uniformly according to a certain proportion, so the testing of the sol method needs to prepare the nanoparticle aggregating agent and the nano enhanced particles for field use, and the process has the problems of complicated operation and spontaneous aggregation of sol in the storage and transportation processes.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a rapid SERS analysis method for energy intake.
In order to achieve the above purpose, the technical scheme of the invention is as follows: a SERS rapid analysis method for energy intake specifically comprises the following steps:
(1) filling a pre-filled liquid: respectively filling silver nanoparticle sol and a nanoparticle aggregating agent into the sol test tubule;
(2) collecting SERS signals: collecting urine of people with different energy intakes, uniformly mixing the urine with the silver nanoparticle sol in the sol test tubule, uniformly mixing the urine with the nanoparticle aggregating agent in the sol test tubule, placing the sol tubule on a Raman instrument, and collecting SERS signals on the lower layer of the tubule;
(3) establishing a urine SERS database: obtaining a scatter distribution diagram corresponding to the surface enhanced Raman spectrum of urine of people with different energy intakes by utilizing a principal component analysis algorithm, carrying out cluster analysis on the energy intakes, and establishing an SERS database;
(4) principal component analysis based on a urine SERS database: the method comprises the following steps:
a. collecting urine of a person to be detected, and collecting an SERS spectral signal;
b. carrying out power-time normalization operation on the collected SERS spectral signals, and calculating a first derivative difference spectrogram; combining the spectrogram with a pre-established SERS database to obtain a new spectral data set;
c. calculating a principal component score matrix from the spectral data set by using a principal component analysis algorithm;
d. and drawing a scatter diagram of the principal component 1(PC1) and the principal component 2(PC2), and marking scatter classification in a database and scatters corresponding to the tested spectrum to obtain an SERS (surface enhanced Raman scattering) principal component analysis scatter distribution diagram of the human urine.
In a preferred embodiment of the present invention, the sol testing small tube in step (1) comprises an upper layer and a lower layer of borosilicate glass small tubes, which are respectively used for containing the silver nanoparticle sol and the nanoparticle aggregating agent, and the upper layer and the lower layer are separated by an elastic membrane, the elastic membrane is used for sealing the lower layer of nanoparticle aggregating agent and carrying the upper layer of silver nano reinforcing particle sol; the top of the small sol testing tube is also provided with a soft rubber cap containing an elastic diaphragm for sealing the small sol testing tube, so that the small sol testing tube is convenient to store and transport; the sol testing small tube is made of glass material, so that the light transmission is facilitated, and the Raman signal of the sample in the tube can be conveniently acquired.
In a preferred embodiment of the present invention, the silver nanoparticle sol in step (1) is prepared by a sodium citrate reduction method, and the specific preparation method is as follows: heating 100mL of 1-5mM silver nitrate solution to boil, adding 5mL of 1 wt% sodium citrate solution once again, and continuously boiling for 3 hours to obtain the silver nanoparticle sol with the particle size of 80-100 nm.
In a preferred embodiment of the present invention, the nanoparticle aggregating agent in step (1) is one or more selected from the group consisting of HCl, NaCl, KCl and NaOH, and the concentration of the nanoparticle aggregating agent is 1mol/L, wherein the most preferred aggregating agent is 1mol/L HCl.
In a preferred embodiment of the present invention, the urine collected in step (2) can be directly used for testing without any pretreatment.
In a preferred embodiment of the present invention, the ratio of the silver nanoparticle sol, the nanoparticle aggregation agent and the urine in the step (2) is 150-450uL:40-120uL:100-300 uL.
In a preferred embodiment of the present invention, the raman spectrometer in step (2) is a portable raman spectrometer, and the laser excitation wavelength is 785 nm.
In a preferred embodiment of the present invention, the SERS database in step (3) is composed of surface enhanced raman spectroscopy data of human urine with different energy intakes.
In a preferred embodiment of the present invention, before the SERS database of urine in step (3) is established, a surface enhanced raman spectrum of urine is normalized to eliminate deviation caused by laser power and acquisition time; and then calculating a first derivative difference spectrum of the spectrum to avoid interference caused by a fluorescence background.
In a preferred embodiment of the present invention, the method for acquiring SERS spectrum signals in step (4) a is the same as that in step (3).
Compared with the prior art, the invention has the beneficial effects that:
1. the sol testing small pipe designed by the invention has the advantages of small volume, light weight, convenience for storage, transportation and use and the like; the inconvenience caused by carrying nano enhanced particle sol and nano particle aggregating agent in field detection can be avoided, and meanwhile, the operation steps are saved, thereby being beneficial to realizing rapid and convenient detection; and can be prepared in batches for ready use.
2. The urine test in the invention does not need pretreatment, the time consumption of the spectral test is short, the result can be obtained in 1 minute by the spectral data analysis, the integral test analysis time does not exceed 2 minutes, and the operation is simple and efficient.
3. The test data and the numerical calculation data obtained by the invention are obtained based on objective test analysis, thereby avoiding errors caused by artificial subjective factors.
4. According to the invention, the relation between the SERS signal of the urine and the energy intake is established by collecting the SERS signal of the urine of people with different energy intakes and carrying out principal component analysis, so that the direct analysis of the SERS signal of the urine can be realized to obtain the information of the energy intake, the complicated and tedious calculation is avoided, and a reference is provided for the energy intake balance of a human body; the method can complete the test without professional technicians and any large-scale instrument equipment; the method is simple to operate, low in cost, fast in speed, portable in instrument and easy to popularize, is an SERS method which is very suitable for rapidly evaluating the energy intake on site, and has very wide application prospect in the field of biomedicine.
Drawings
FIG. 1 is a schematic view of a sol test cuvette designed according to the present invention, in which FIG. A is a perspective view and FIG. B is a cross-sectional view;
wherein: 1-soft rubber cap 1, 2-upper layer borosilicate glass small tube, 3-lower layer borosilicate glass small tube, 4-elastic diaphragm in the middle of soft rubber cap, 5-elastic diaphragm between two layers of borosilicate glass small tubes
FIG. 2 is a scanning electron micrograph of silver nanoparticles used in the present invention;
FIG. 3 is a diagram showing an ultraviolet-visible absorption spectrum of a silver nanoparticle sol prepared according to the present invention;
FIG. 4 is an average surface enhanced Raman spectrum of human urine taken at different energy intakes, measured in accordance with the present invention, for a group A of 2300 kcal/day and a group B of 1500 kcal/day;
FIG. 5 is a scatter plot of principal component analysis of urine at different energy intakes measured in accordance with the present invention, with group A having an energy intake of 2300 kcal/day; group B energy intake was 1500 kcal/day.
Detailed Description
The invention is further explained below with reference to the figures and the specific embodiments.
1. Design of sol testing tubule
The sol test small tube comprises a soft rubber cap 1 (used for sealing a reagent), an upper layer high borosilicate glass small tube 2 (used for containing nano reinforced particles) and a lower layer high borosilicate glass small tube 3 (used for containing a nano particle aggregating agent), as shown in the attached figure 1 (A). The sol testing small tube also comprises an elastic diaphragm 4 in the middle of the soft rubber cap and an elastic diaphragm 5 between two layers of borosilicate glass small tubes, which can resist certain pressure, bear and seal reagents and facilitate the pipette tip to puncture the elastic diaphragm to realize the uniform mixing of the reagents, as shown in figure 1 (B). The sol testing small tube can be filled with a pre-filled liquid for use, and the specific steps are as follows: transferring the prepared nanoparticle aggregating agent into the lower layer of the sol test small tube by using a liquid transfer gun, sealing the sol test small tube by using an elastic diaphragm, and connecting the upper and lower layers of high borosilicate glass small tubes by using a heat shrink tube; transferring the silver nano sol into the upper layer of the sol testing tubule, and sealing the upper layer of the sol testing tubule by using a soft rubber cap with an elastic diaphragm to finish the whole process of filling and pre-filling liquid; wherein the proportion of the silver nanoparticle sol, the nanoparticle aggregating agent and the urine is 150-450uL:40-120uL:100-300 uL.
2. Preparation of silver nanoparticle sol
The silver nanoparticle sol is synthesized by adopting a sodium citrate reduction method, heating and boiling 100mL of 1-5mM silver nitrate solution, adding 5mL of 1 wt% sodium citrate solution once again, and continuously boiling for 3 hours to obtain the silver sol, wherein the particle size of the silver nanoparticles is 80-100 nm.
3. Collection of urine
Experiment A group of participants had 11 persons in total, and intake energy of 2300 kcal/day; the trial participants in group B had 11 total individuals who ingested 1500 kcal/day of energy. Each group of experiments takes 3 days, morning urine of the subject is collected, each 3mL part, in order to reach human body metabolic balance, the first two days are taken as a metabolic buffer period, samples are not collected, and the urine samples in the third day stable period are selected for signal acquisition. The urine can be directly used for subsequent detection without any pretreatment.
SERS spectral signal acquisition
Absorbing and collecting 200uL urine by using a pipette, uniformly mixing the urine with nano enhanced particles in the upper layer of a sol test small tube, uniformly mixing the urine with a nano particle aggregating agent in the lower layer of the sol test small tube, and collecting the sol test small tube with the lower layer of 300-1200 cm by using a portable Raman instrument -1 Obtaining the surface enhanced Raman spectrum of the urine by using Raman signals in the wave number range; the parameters for the test were: the excitation light wavelength is 785nm, the laser power is 100mW, the integration time is 3-8 s, and the accumulation times are 2 times.
5. Establishment of urine SERS database based on SERS spectrogram
Performing principal component analysis on the collected SERS spectrogram of the urine, and establishing a database; in order to avoid deviation caused by signal fluctuation in the acquisition process, normalization operation needs to be carried out on all spectral data, namely, the spectral intensity value is divided by the acquisition time and divided by the acquisition power; the calculated spectral atlas may be subjected to principal component analysis. The following is a specific calculation procedure:
(1) calculating a first derivative difference spectrum for all spectra to eliminate a fluorescence background;
(2) performing principal component analysis on the first derivative difference spectrum (A group and B group) by using a statistical and machine learning toolkit in MATLAB R2017a software;
(3) a scatter plot of principal component 1(PC1) and principal component 2(PC2) was drawn, and the total contribution ratio of the two principal components was calculated.
6. Principal component analysis based on urine SERS database
The method comprises the following specific steps:
(1) collecting urine of a new person to be tested, transferring a 200uL urine sample by using a liquid transfer gun, puncturing an elastic diaphragm of a soft rubber cap of a sol test small tube, realizing sample application, uniformly mixing the urine and silver nanoparticle sol, puncturing a middle elastic diaphragm by using a liquid transfer suction head, uniformly mixing a urine sample-silver nanoparticle sol mixture and a nanoparticle aggregating agent, and performing a test according to the condition of the acquisition of the SERS spectral signal;
(2) carrying out power-time normalization operation on the collected SERS spectrum, and calculating a first derivative difference spectrogram; combining the spectrogram with a pre-established SERS database to obtain a new spectral data set;
(3) calculating a principal component score matrix of the spectral data set by using a principal component analysis algorithm;
(4) and drawing a scatter diagram of the principal component 1(PC1) and the principal component 2(PC2), marking scatter classification in a database and scatter corresponding to a tested spectrum, and obtaining an obtained SERS principal component analysis scatter distribution diagram of the human urine, namely the measurement result of the invention.
The integrated SERS sol detection kit has the advantages of light weight, small volume, great convenience for storage, transportation and use of sol and the like, is used for collecting a human urine SERS spectrogram, obtains a visual scattered point distribution diagram by combining a principal component analysis algorithm, and can directly judge the level of human energy intake; the obtained test data and numerical calculation data are obtained based on objective test analysis, so that errors caused by artificial subjective factors are avoided; the urine test does not need pretreatment, the time consumption of the spectral test is short, the result can be obtained within 1 minute by the spectral data analysis, and the overall test analysis time is not more than 2 minutes. Therefore, the invention has very wide application prospect in the biomedical field.
Example 1
(1) Preparing silver nano sol: the synthesis of the silver nano sol adopts a sodium citrate reduction method, 100mL of 1.5mM silver nitrate solution is heated and boiled, 5mL of 1 wt% sodium citrate solution is added once, and the boiling is continued for 3 hours, so that the silver sol is obtained.
(2) Sol test vials were filled with prepad liquid: and (3) transferring 80uL of the nanoparticle aggregating agent into the lower layer of the sol test small tube by using a liquid transfer gun, sealing the sol test small tube by using an elastic diaphragm, and connecting the upper and lower layers of the borosilicate glass small tubes by using a heat-shrinkable tube. And then transferring the prepared 300uL of silver nano sol into the upper layer of the sol testing tubule by using a liquid transfer gun, and covering a soft rubber cap after the completion of the liquid transfer gun to complete the whole process of filling and pre-filling the liquid.
(3) Collecting urine: experiment A group of participants had 11 persons in total, and intake energy of 2300 kcal/day; the trial participants in group B had 11 total individuals who ingested 1500 kcal/day of energy. Each group of experiments takes 3 days, morning urine of the subject is collected, each 3mL part, in order to reach human body metabolic balance, the first two days are taken as a metabolic buffer period, samples are not collected, and the urine samples in the third day stable period are selected for signal acquisition. The urine can be directly used for subsequent detection without any pretreatment.
(4) Collecting SERS signals: absorbing the collected 200uL urine by using a pipette gun, simultaneously puncturing an elastic diaphragm at the top end of the upper layer in the sol testing small tube by using the pipette gun head, uniformly mixing the elastic diaphragm with nano-reinforcing particles in the elastic diaphragm, puncturing an elastic diaphragm 5 in the sol testing small tube, uniformly mixing the elastic diaphragm with a nano-particle aggregating agent in the lower layer of the sol testing small tube, and finally collecting the lower layer of the sol testing small tube at 300-1200 cm by using a portable Raman instrument -1 And obtaining the surface enhanced Raman spectrum of the urine by using the Raman signal in the wave number range. The parameters tested were: the wavelength of the excitation light is 785nm, the laser power is 100mW, the integration time is 5s, and the accumulation times are 2 times.
(5) Establishing an SERS database of urine: the SERS spectra of the two collected groups of urine were calculated as the average spectra, respectively, as shown in fig. 4. And (3) performing principal component analysis on all SERS spectrograms, wherein before a database is established, normalization operation needs to be performed on all spectral data to avoid deviation caused by signal fluctuation in the acquisition process, namely, dividing the spectral intensity value by the acquisition time and dividing the spectral intensity value by the acquisition power. The scatter plot obtained is shown in FIG. 5.
(6) Principal component analysis based on a urine SERS database: and collecting the urine SERS spectrum of the person to be detected, and then carrying out power and time normalization and difference subtraction background pretreatment. And synthesizing the processed data with an SERS database, analyzing the main components, obtaining a scatter distribution diagram, and marking the positions of the test scatter points in the diagram.
Example 2
(1) Preparing silver nano sol: the silver nano sol is synthesized by adopting a sodium citrate reduction method, heating and boiling 100mL of 2mM silver nitrate solution, adding 5mL of 1 wt% sodium citrate solution once again, and continuously boiling for 3 hours to obtain the silver sol, wherein the particle size of the silver nanoparticles is 80-100 nm, and the ultraviolet visible absorption peak of the silver nanoparticles is positioned at 404nm, as shown in attached figures 2 and 3.
(2) Sol test vials were filled with prepad liquid: and (3) transferring 50uL of the nanoparticle aggregating agent into the lower layer of the sol test small tube by using a liquid transfer gun, sealing the sol test small tube by using an elastic diaphragm, and connecting the upper and lower layers of the borosilicate glass small tubes by using a heat-shrinkable tube. And then transferring the prepared 300uL of silver nano sol into the upper layer of the sol testing tubule by using a liquid transfer gun, and covering a soft rubber cap after the completion of the liquid transfer gun to complete the whole process of filling and pre-filling the liquid.
(3) Collecting urine: experiment A group of participants had 11 persons in total, and intake energy of 2300 kcal/day; the trial participants in group B had 11 total individuals who ingested 1500 kcal/day of energy. Each group of experiments takes 3 days, morning urine of a subject is collected, each 3mL of urine is collected, in order to achieve human body metabolic balance, the first two days are taken as a metabolic buffer period, samples are not collected, and the urine samples in the third day of stable period are selected for signal acquisition; the urine can be directly used for subsequent detection without any pretreatment.
(4) Collecting SERS signals: absorbing the collected 200uL urine by using a pipette gun, simultaneously puncturing an elastic diaphragm at the top end of the upper layer in the sol testing small tube by using the pipette gun head, uniformly mixing the elastic diaphragm with nano-reinforcing particles in the elastic diaphragm, puncturing an elastic diaphragm 5 in the sol testing small tube, uniformly mixing the elastic diaphragm with a nano-particle aggregating agent in the lower layer of the sol testing small tube, and finally collecting the lower layer of the sol testing small tube at 300-1200 cm by using a portable Raman instrument -1 And obtaining the surface enhanced Raman spectrum of the urine by using the Raman signal in the wave number range. The parameters tested were: the wavelength of the excitation light is 785nm, the laser power is 100mW, the integration time is 6s, and the accumulation times are 2 times.
(5) Establishing an SERS database of urine: and calculating average spectra of the collected SERS spectrograms of the two groups of urine respectively. And (3) performing principal component analysis on all SERS spectrograms, wherein before a database is established, normalization operation needs to be performed on all spectral data to avoid deviation caused by signal fluctuation in the acquisition process, namely, dividing the spectral intensity value by the acquisition time and dividing the spectral intensity value by the acquisition power.
(6) Principal component analysis based on a urine SERS database: and collecting the urine SERS spectrum of the person to be detected, and then carrying out power and time normalization and difference subtraction background pretreatment. And synthesizing the processed data with an SERS database, analyzing the main components, obtaining a scatter distribution diagram, and marking the positions of the test scatter points in the diagram.
The above examples are only intended to further illustrate the method for SERS rapid analysis of energy intake of the present invention, but the present invention is not limited to the examples, and any simple modification, equivalent change and modification of the above examples according to the technical spirit of the present invention fall within the protection scope of the technical solution of the present invention.
Claims (10)
1. A method for SERS rapid analysis of energy intake is characterized by comprising the following steps:
(1) filling a pre-filled liquid: respectively filling silver nanoparticle sol and a nanoparticle aggregating agent into the sol test tubule;
(2) collecting SERS signals: collecting urine of people with different energy intakes, uniformly mixing the urine with the silver nanoparticle sol in the sol test tubule, uniformly mixing the urine with the nanoparticle aggregating agent in the sol test tubule, placing the sol tubule on a Raman instrument, and collecting an SERS signal;
(3) establishing a urine SERS database: acquiring a scatter distribution diagram corresponding to the surface enhanced Raman spectrum of the urine of people with different energy intakes by using a principal component analysis algorithm, performing cluster analysis on the energy intakes, and establishing an SERS database;
(4) principal component analysis based on a urine SERS database: the method comprises the following steps:
a. collecting urine of a person to be detected, and collecting an SERS spectral signal;
b. carrying out power-time normalization operation on the collected SERS spectral signals, and calculating a first derivative difference spectrogram; combining the spectrogram with a pre-established SERS database to obtain a new spectral data set;
c. calculating a principal component score matrix from the spectral data set by using a principal component analysis algorithm;
d. and drawing a scatter diagram of the main component 1(PC1) and the main component 2(PC2), and marking scatter classification in a database and scatter corresponding to the tested spectrum, thereby obtaining an SERS main component analysis scatter distribution diagram of the human urine.
2. The method for SERS rapid analysis of energy intake according to claim 1, wherein the sol test tube in step (1) comprises an upper borosilicate glass tube and a lower borosilicate glass tube, and the upper borosilicate glass tube and the lower borosilicate glass tube are separated by an elastic diaphragm; the top of the sol test small tube is also provided with a soft rubber cap containing an elastic diaphragm; the sol test tube is made of glass.
3. The method for SERS rapid analysis of energy intake according to claim 1, wherein the silver nanoparticle sol and the nanoparticle aggregating agent in step (1) are respectively contained in the upper and lower borosilicate glass vials of the sol test vial.
4. The method for SERS rapid analysis of energy intake according to claim 1, wherein the silver nanoparticle sol in step (1) is prepared by sodium citrate reduction.
5. The method for SERS rapid analysis of energy intake according to claim 1, wherein the nanoparticle aggregating agent in step (1) is one or more of HCl, NaCl, KCl and NaOH.
6. The method for SERS rapid analysis of energy intake as claimed in claim 1, wherein the ratio of the silver nanoparticle sol, the nanoparticle aggregating agent and the urine in step (2) is 150-450uL:40-120uL:100-300 uL.
7. The method for SERS rapid analysis of energy intake according to claim 1, wherein the urine collected in step (2) is directly used for testing without any pretreatment.
8. The method for SERS rapid analysis of energy intake according to claim 1, wherein the raman spectrometer in step (2) is a portable raman spectrometer, and the laser excitation wavelength is 785 nm.
9. The method for SERS rapid analysis of energy intake according to claim 1, wherein before the SERS database of urine in step (3) is established, the surface enhanced Raman spectrum of urine is normalized, and then a first derivative difference spectrum of the spectrum is calculated.
10. The method for SERS rapid analysis of energy intake according to claim 1, wherein the step (4) a is performed in the same manner as step (3).
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