CN114949283B - Stem cell culture dish disinfection system and disinfection method - Google Patents
Stem cell culture dish disinfection system and disinfection method Download PDFInfo
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- CN114949283B CN114949283B CN202210829653.5A CN202210829653A CN114949283B CN 114949283 B CN114949283 B CN 114949283B CN 202210829653 A CN202210829653 A CN 202210829653A CN 114949283 B CN114949283 B CN 114949283B
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 87
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 24
- 238000004113 cell culture Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 110
- 230000009471 action Effects 0.000 claims abstract description 6
- 238000007599 discharging Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/24—Apparatus using programmed or automatic operation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/12—Apparatus for isolating biocidal substances from the environment
- A61L2202/121—Sealings, e.g. doors, covers, valves, sluices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/14—Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a stem cell culture dish sterilizing system and a sterilizing method, wherein the stem cell culture dish sterilizing system comprises a sterilizing rack assembly and a sterilizing box with an opening at the upper end, wherein the left side of the sterilizing box is provided with a left arc-shaped door plate, and the right side of the sterilizing box is provided with a right arc-shaped door plate; the sterilizing box is rotationally connected with a left rotating wheel and a right rotating wheel; a high-temperature disinfection assembly and an ultraviolet disinfection assembly are arranged in the top cover; the first rotating wheel positioned in the first rotating hole is rotationally connected to the bottom plate under the high-temperature sterilizing component, and the second rotating wheel positioned in the second rotating hole is arranged under the ultraviolet sterilizing component; the sterilizing rack assembly is positioned in the sterilizing box and used for placing the culture dish, and under the action of the left rotating wheel, the first rotating wheel, the second rotating wheel and the right rotating wheel, the sterilizing rack assembly drives the culture dish to sequentially pass through the high-temperature sterilizing assembly and the ultraviolet sterilizing assembly; the stem cell culture dish disinfection system is simple in structure and can efficiently disinfect culture dishes.
Description
Technical Field
The invention relates to the technical field of stem cells, in particular to a stem cell culture dish disinfection system and a stem cell culture dish disinfection method.
Background
A culture dish is a laboratory vessel for microorganism or cell culture, generally consisting of a bottom and a cover of a disc, mainly made of plastic and glass, which is advantageous for the observation and manipulation of microorganisms due to its transparency. The culture dish is mainly used for microorganism culture, iron wall culture of animal cells, laboratory inoculation, streaking, bacteria separation and the like, has been applied to stem cell experiments for decades, and is an essential culture device in stem cell experiments. In the prior art, in order to ensure that the experimental result has no external interference, the culture dish needs to be disinfected before being used, so that the influence of harmful bacteria on stem cell experiments is reduced, but the following problems generally occur when the culture dish is disinfected by the existing equipment:
1. When the existing equipment is used for sterilizing the culture dish, the culture dish cannot be added in midway for sterilization in the sterilization process because the sterilization equipment is required to be completely sealed;
2. when the prior equipment is used for sterilizing the culture dish, the culture dish is generally sterilized by ultraviolet lamp irradiation, but because the irradiation of ultraviolet rays to the culture dish is uneven, the dead angle of illumination exists, so that the sterilization effect of the culture dish is not ideal, and the sterilization is not thorough.
Disclosure of Invention
The invention aims to provide a stem cell culture dish disinfection system which is simple in structure and can efficiently disinfect culture dishes.
In order to achieve the above purpose, the present invention provides the following technical solutions: the stem cell culture dish disinfection system comprises a disinfection frame assembly and a disinfection box with an opening at the upper end, wherein the disinfection box comprises a main box body in the middle, a left semicircular box body arranged at the left end of the main box body and a right semicircular box body arranged at the right end of the main box body, a left arc-shaped door plate is arranged at the left side of the left semicircular box body, and a right arc-shaped door plate is arranged at the right side of the right semicircular box body; the bottom of the disinfection box is provided with a left turning hole, a first turning hole, a second turning hole and a right turning hole in sequence from left to right; a top cover is fixedly arranged at the upper end of the disinfection box, and a bottom plate which is blocked on the left turning hole, the first turning hole, the second turning hole and the right turning hole is fixedly arranged at the lower end of the disinfection box; a left rotating wheel positioned in the left rotating hole is rotationally connected to the center of the left semicircular box body in the sterilizing box, and a right rotating wheel positioned in the right rotating hole is rotationally connected to the center of the right semicircular box body in the sterilizing box; the right side of the left rotating wheel in the top cover is provided with a high-temperature disinfection assembly, and the left side of the right rotating wheel is provided with an ultraviolet disinfection assembly; the first rotating wheel positioned in the first rotating hole is rotationally connected to the bottom plate under the high-temperature sterilizing component, and the second rotating wheel positioned in the second rotating hole is arranged under the ultraviolet sterilizing component; the disinfection frame assembly is located in the disinfection box and used for placing the culture dish, and under the action of the left rotating wheel, the first rotating wheel, the second rotating wheel and the right rotating wheel, the disinfection frame assembly drives the culture dish to sequentially pass through the high-temperature disinfection assembly and the ultraviolet disinfection assembly.
Further, a left rotating shaft at the center of the left rotating hole is rotatably connected between the top cover and the bottom plate, the left rotating wheel is fixedly arranged on the left rotating shaft, a plurality of left radial plates are uniformly arranged on the outer side of the left rotating wheel along the circumferential direction at intervals, and when the left radial plates rotate around the left rotating shaft, the left radial plates are abutted against the inner side wall of the left semicircular box body; the rotary type right rotary box is characterized in that a right rotary shaft at the center of a right rotary hole is rotatably connected between the top cover and the bottom plate, the right rotary wheel is fixedly arranged on the right rotary shaft, a plurality of right radial plates are uniformly arranged on the outer side of the right rotary wheel along the circumferential interval, and when the right radial plates rotate around the right rotary shaft, the right radial plates are abutted against the inner side wall of the right semicircular box body.
Further, a plurality of left avoidance grooves for the left radial plate to extend in are uniformly formed in the outer circumferential side of the first rotating wheel along the circumferential interval, and a plurality of right avoidance grooves for the right radial plate to extend in are uniformly formed in the outer circumferential side of the second rotating wheel along the circumferential interval.
Further, the bottom plate is rotatably connected with a first rotating shaft at the center of the first rotating hole, and is rotatably connected with a second rotating shaft at the center of the second rotating hole, the first rotating wheel is fixedly arranged on the first rotating shaft, and the second rotating wheel is fixedly arranged on the second rotating shaft.
Further, the lower end of the left rotating shaft extends out of the bottom plate and is fixedly provided with a left gear, the lower end of the first rotating shaft extends out of the bottom plate and is fixedly provided with a first gear meshed with the left gear, the lower end of the second rotating shaft extends out of the bottom plate and is fixedly provided with a second gear meshed with the first gear, and the lower end of the right rotating shaft extends out of the bottom plate and is fixedly provided with a right gear meshed with the second gear; the lower extreme of bottom plate is equipped with the support frame, fixedly on the support frame be equipped with be used for driving left pivot pivoted first motor.
Further, a left track is formed between the left rotating hole and the left rotating wheel, a first track is formed between the first rotating hole and the first rotating wheel, a second track is formed between the second rotating hole and the second rotating wheel, and a right track is formed between the right rotating hole and the right rotating wheel; the disinfection frame assembly comprises a shaft rod, a left rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the left rotating wheel, a first rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the first rotating wheel, a second rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the second rotating wheel, and a right rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the right rotating wheel; the upper end of the shaft lever is provided with a limiting convex edge which is positioned above the first rotating wheel and the second rotating wheel and is used for being clamped into the outer sides of the left rotating wheel and the right rotating wheel, the upper end of the limiting convex edge is provided with an upward extending mounting plate, and a plurality of mounting holes for placing culture dishes are formed in the mounting plate; a third gear is fixedly arranged at the upper end of the mounting plate;
The connecting part of the left rotating hole and the first rotating hole in the bottom plate is provided with a first rotating rod, the upper end of the first rotating rod is fixedly provided with a first shifting plate, the lower end of the first rotating rod is fixedly provided with a first connecting rod, the lower end of the bottom plate is hinged with a first electric push rod, and the telescopic end of the first electric push rod is hinged with the first connecting rod; when the first electric push rod is completely contracted, the left rotating wheel drives the shaft rod to move in the left track, and when the first electric push rod is completely extended, the first shifting plate guides the shaft rod to move in the first track from the left track;
A second rotating rod is arranged at the joint of the first rotating hole and the second rotating hole in the bottom plate, a second shifting plate is fixedly arranged at the upper end of the second rotating rod, a second connecting rod is fixedly arranged at the lower end of the second rotating rod, a second electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the second electric push rod is hinged to the second connecting rod; when the second electric push rod is completely contracted, the first rotating wheel drives the shaft rod to move in the first track, and when the second electric push rod is completely extended, the second shifting plate guides the shaft rod to move from the first track into the second track;
A third rotating rod is arranged at the joint of the second rotating hole and the right rotating hole in the bottom plate, a third shifting plate is fixedly arranged at the upper end of the third rotating rod, a third connecting rod is fixedly arranged at the lower end of the third rotating rod, a third electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the third electric push rod is hinged to the third connecting rod; when the third electric push rod is completely extended, the second rotating wheel drives the shaft rod to move in the second track, and when the first electric push rod is completely contracted, the third shifting plate guides the shaft rod to move in the right track from the second track.
Further, the side wall of the mounting hole is provided with a mounting groove, and a pressing block and a spring plate for forcing the pressing block to extend into the mounting hole are hinged in the mounting groove; the briquetting is used for compressing the culture dish in the mounting hole.
Further, the high-temperature disinfection assembly comprises a convex column which is downwards arranged on the top cover along the vertical direction, a high-temperature air inlet hole which is vertically arranged is arranged in the convex column, and a high-temperature air outlet hole which is communicated with the high-temperature air inlet hole is arranged at the outer side of the circumference of the convex column; the outside symmetry of projection is equipped with first incomplete gear, first incomplete gear is used for with third gear meshing drive axostylus axostyle rotation.
Further, the ultraviolet disinfection assembly comprises a convex plate which is downwards arranged on the top cover along the vertical direction, and a plurality of ultraviolet lamps are fixedly arranged on the outer side of the convex plate; the outer side of the convex plate is fixedly provided with a second incomplete gear, and the second incomplete gear is used for being meshed with a third gear to drive the shaft rod to rotate.
The invention also discloses a method for sterilizing the culture dish by using the stem cell culture dish sterilizing system, which comprises the following steps:
S1: introducing high-temperature air from the high-temperature air inlet hole, enabling the high-temperature air to be sprayed out through the high-temperature air outlet hole and enter the interior of the sterilizing box, and then opening all ultraviolet lamps;
S2: placing a culture dish to be sterilized by opening the left arc-shaped door plate in a sterilizing rack assembly, closing the left arc-shaped door plate, stopping the rotation of the left rotating wheel when the left rotating wheel is controlled to drive the next sterilizing rack assembly to face the left arc-shaped door plate, opening the left arc-shaped door plate, then manually installing the culture dish in the next sterilizing rack assembly, and sequentially operating until all culture dishes are completely fed;
s3: controlling the left rotating wheel to drive the sterilizing frame assembly to move to the outer side of the first rotating wheel;
S4: the first rotating wheel drives the sterilizing rack assembly to rotate, and the high-temperature sterilizing assembly sprays high-temperature air on the surface of the culture dish to sterilize the culture dish at high temperature;
S5: when the culture dish is sterilized at high temperature, the first rotating wheel is controlled to drive the sterilizing rack assembly to be transported to the outer side of the second rotating wheel;
s6: the second rotating wheel drives the sterilizing rack assembly to rotate, and ultraviolet rays emitted by the ultraviolet sterilizing assembly irradiate the surface of the culture dish to sterilize the culture dish by ultraviolet rays;
S7: after ultraviolet sterilization of the culture dish is completed, controlling the second rotating wheel to drive the sterilizing rack assembly to move to the outer side of the right rotating wheel;
S8: when the sterilizing rack assembly rotates to be aligned with the right arc-shaped door plate, the right arc-shaped door plate is opened to take down the sterilized culture dish, and discharging is completed.
Advantageous effects
Compared with the prior art, the technical scheme of the invention has the following advantages:
1. The left rotating wheel with the left radial plate is arranged at the left arc-shaped door plate, the right rotating wheel with the right radial plate is arranged at the right arc-shaped door plate, so that when a culture dish to be disinfected is fed, or when the disinfected culture dish is fed, the disinfection part of the equipment can be closed, the disinfection part is not contacted with the outside, and when the culture dish is fed and fed, the disinfection of the culture dish is not delayed, and in the disinfection process, the culture dish can be still added into the equipment, and meanwhile, the culture dish after the disinfection is finished can be fed;
2. the left rotating wheel, the first rotating wheel, the second rotating wheel and the right rotating wheel are arranged in the equipment, and the first shifting plate, the second shifting plate and the third shifting plate are arranged at the same time, so that the shaft lever can be selected to enter the left track, the second track or the right track, and further the disinfection cycle is automatically carried out, and the device is convenient and quick;
3. When the mounting plate is sterilized in the high-temperature sterilization zone and the ultraviolet sterilization zone through the first incomplete gear and the second incomplete gear, the first incomplete gear and the second incomplete gear are matched with a third gear on the mounting plate respectively to drive the mounting plate to rotate, so that the turnover of a culture dish on the mounting plate is controlled, the sterilization dead angle is reduced, and the sterilization quality is improved;
4. through setting up high temperature venthole and ultraviolet lamp for be provided with high temperature disinfection district and ultraviolet disinfection district in the disinfect box, thereby carry out the disinfection of ultraviolet ray after the first high temperature disinfection to the culture dish of installing on the mounting panel, disinfect many times, promote disinfection effect.
Drawings
FIG. 1 is a cross-sectional view of the present invention;
FIG. 2 is a cross-sectional view taken along the direction A-A in FIG. 1 in accordance with the present invention;
FIG. 3 is a cross-sectional view taken along the direction B-B in FIG. 1 in accordance with the present invention;
FIG. 4 is a cross-sectional view taken along the direction C-C in FIG. 1 in accordance with the present invention;
FIG. 5 is a sectional view taken along the direction D-D in FIG. 1 in accordance with the present invention;
fig. 6 is a sectional view taken along the direction E-E in fig. 1 in accordance with the present invention.
Detailed Description
Referring to fig. 1-6, a stem cell culture dish sterilizing system comprises a sterilizing rack assembly and a sterilizing box 1 with an opening at the upper end, wherein the sterilizing box 1 comprises a main box body in the middle, a left semicircular box body arranged at the left end of the main box body, and a right semicircular box body arranged at the right end of the main box body, the left side of the left semicircular box body is provided with a left arc-shaped door plate 21, and the right side of the right semicircular box body is provided with a right arc-shaped door plate 22; the bottom of the sterilizing box 1 is provided with a left turning hole 1c, a first turning hole 1d, a second turning hole 1e and a right turning hole 1f in sequence from left to right; a top cover 1a is fixedly arranged at the upper end of the disinfection box 1, and a bottom plate 1b blocked on the left turning hole 1c, the first turning hole 1d, the second turning hole 1e and the right turning hole 1f is fixedly arranged at the lower end of the disinfection box; a left rotating wheel 41 positioned in the left rotating hole 1c is rotatably connected to the center of the left semicircular box body in the sterilizing box 1, and a right rotating wheel 42 positioned in the right rotating hole 1f is rotatably connected to the center of the right semicircular box body; a high-temperature sterilization component is arranged on the right side of the left rotating wheel 41 in the top cover 1a, and an ultraviolet sterilization component is arranged on the left side of the right rotating wheel 42; a first rotating wheel 51 positioned in the first rotating hole 1d is rotationally connected to the bottom plate 1b under the high-temperature sterilization component, and a second rotating wheel 52 positioned in the second rotating hole 1e is arranged under the ultraviolet sterilization component; the sterilizing rack assembly is positioned in the sterilizing box 1 and used for placing the culture dishes, and under the action of the left rotating wheel 41, the first rotating wheel 51, the second rotating wheel 52 and the right rotating wheel 42, the sterilizing rack assembly drives the culture dishes to sequentially pass through the high-temperature sterilizing assembly and the ultraviolet sterilizing assembly.
A left rotating shaft 4a at the center of the left rotating hole 1c is rotatably connected between the top cover 1a and the bottom plate 1b, the left rotating wheel 41 is fixedly installed on the left rotating shaft 4a, a plurality of left radial plates 411 are uniformly arranged on the outer side of the left rotating wheel 41 along the circumferential direction at intervals, and when the left radial plates 411 rotate around the left rotating shaft 4a, the left radial plates 411 are abutted against the inner side wall of the left semicircular box body; the right rotating shaft 4b at the center of the right rotating hole 1f is rotatably connected between the top cover 1a and the bottom plate 1b, the right rotating wheel 42 is fixedly installed on the right rotating shaft 4b, a plurality of right radial plates 421 are uniformly arranged on the outer side of the right rotating wheel 42 along the circumferential interval, and when the right radial plates 421 rotate around the right rotating shaft 4b, the right radial plates 421 are abutted against the inner side wall of the right semicircular box body.
The outer circumference of the first rotating wheel 51 is uniformly provided with a plurality of left avoidance grooves 5a for the left radial plate 411 to extend in along the circumferential interval, and the outer circumference of the second rotating wheel 52 is uniformly provided with a plurality of right avoidance grooves 5b for the right radial plate 421 to extend in along the circumferential interval. The bottom plate 1b is rotatably connected with a first rotating shaft 5c at the center of the first rotating hole 1d, and is rotatably connected with a second rotating shaft 5d at the center of the second rotating hole 1e, the first rotating wheel 51 is fixedly arranged on the first rotating shaft 5c, and the second rotating wheel 52 is fixedly arranged on the second rotating shaft 5 d. The lower end of the left rotating shaft 4a extends out of the bottom plate 1b and is fixedly provided with a left gear 61, the lower end of the first rotating shaft 5c extends out of the bottom plate 1b and is fixedly provided with a first gear 62 meshed with the left gear 61, the lower end of the second rotating shaft 5d extends out of the bottom plate 1b and is fixedly provided with a second gear 63 meshed with the first gear 62, and the lower end of the right rotating shaft 4b extends out of the bottom plate 1b and is fixedly provided with a right gear 64 meshed with the second gear 63; the lower extreme of bottom plate 1b is equipped with support frame 1h, fixedly on the support frame 1h be equipped with be used for driving left pivot 4a pivoted first motor 8.
A left rail 171 is formed between the left turning hole 1c and the left turning wheel 41, a first rail 172 is formed between the first turning hole 1d and the first turning wheel 51, a second rail 173 is formed between the second turning hole 1e and the second turning wheel 52, and a right rail 174 is formed between the right turning hole 1f and the right turning wheel 42; the sterilizing rack assembly comprises a shaft lever 34, a left rotating groove 413 for the shaft lever 34 to extend is formed on the outer circumference of the left rotating wheel 41, a first rotating groove 511 for the shaft lever 34 to extend is formed on the outer circumference of the first rotating wheel 51, a second rotating groove 521 for the shaft lever 34 to extend is formed on the outer circumference of the second rotating wheel 52, and a right rotating groove 423 for the shaft lever 34 to extend is formed on the outer circumference of the right rotating wheel 42; the upper end of the shaft lever 34 is provided with a limit convex edge 301 which is positioned above the first rotating wheel 51 and the second rotating wheel 52 and is used for being clamped into the outer sides of the left rotating wheel 41 and the right rotating wheel 42, the upper end of the limit convex edge 301 is provided with an upward extending mounting plate 31, and a plurality of mounting holes 311 for placing culture dishes are arranged in the mounting plate 31; a third gear 33 is fixedly arranged at the upper end of the mounting plate 31; the side wall of the mounting hole 311 is provided with a mounting groove 3a, a pressing block 312 is hinged in the mounting groove 3a, and a spring plate 313 for forcing the pressing block 312 to extend into the mounting hole 311; the pressing block 312 is used for pressing the culture dish in the mounting hole 311.
A first rotating rod 9a is arranged at the joint of the left rotating hole 1c and the first rotating hole 1d in the bottom plate 1b, a first shifting plate 911 is fixedly arranged at the upper end of the first rotating rod 9a, a first connecting rod 912 is fixedly arranged at the lower end of the first rotating rod, a first electric push rod 913 is hinged to the lower end of the bottom plate 1b, and the telescopic end of the first electric push rod 913 is hinged to the first connecting rod 912; when the first electric push rod 913 is fully retracted, the left rotating wheel 41 drives the shaft lever 34 to move in the left rail 171, and when the first electric push rod 913 is fully extended, the first shifting plate 911 guides the shaft lever 34 to move from the left rail 171 into the first rail 172; a second rotating rod 9b is arranged at the joint of the first rotating hole 1d and the second rotating hole 1e in the bottom plate 1b, a second shifting plate 921 is fixedly arranged at the upper end of the second rotating rod 9b, a second connecting rod 922 is fixedly arranged at the lower end of the second rotating rod, a second electric push rod 923 is hinged to the lower end of the bottom plate 1b, and the telescopic end of the second electric push rod 923 is hinged to the second connecting rod 922; when the second electric push rod 923 is completely contracted, the first rotating wheel 51 drives the shaft lever 34 to move in the first track 172, and when the second electric push rod 923 is completely extended, the second shifting plate 921 guides the shaft lever 34 to move from the first track 172 into the second track 173; a third rotating rod 9c is arranged at the joint of the second rotating hole 1e and the right rotating hole 1f in the bottom plate 1b, a third shifting plate 931 is fixedly arranged at the upper end of the third rotating rod 9c, a third connecting rod 932 is fixedly arranged at the lower end of the third rotating rod, a third electric push rod 933 is hinged at the lower end of the bottom plate 1b, and the telescopic end of the third electric push rod 933 is hinged with the third connecting rod 932; when the third electric push rod 933 is fully extended, the second rotating wheel 52 drives the shaft lever 34 to move in the second track 173, and when the first electric push rod 913 is fully retracted, the third poking plate 931 guides the shaft lever 34 to move from the second track 173 into the right track 174.
The high-temperature disinfection assembly comprises a convex column 11 which is downwards arranged on the top cover 1a along the vertical direction, a high-temperature air inlet 11a which is vertically arranged is arranged in the convex column 11, and a high-temperature air outlet 11b which is communicated with the high-temperature air inlet 11a is arranged at the outer side of the circumference of the convex column 11; the outer side of the convex column 11 is symmetrically provided with a first incomplete gear 13, and the first incomplete gear 13 is used for meshing with a third gear 33 to drive the shaft lever 34 to rotate.
The ultraviolet disinfection assembly comprises a convex plate 7 which is downwards arranged on the top cover 1a along the vertical direction, and a plurality of ultraviolet lamps 71 are fixedly arranged on the outer side of the convex plate 7; a second incomplete gear 15 is fixedly arranged on the outer side of the convex plate 7, and the second incomplete gear 15 is used for meshing with a third gear 33 to drive the shaft lever 34 to rotate.
The embodiment also provides a method for sterilizing the culture dish by using the stem cell culture dish sterilization system, which comprises the following steps:
s1: high-temperature air is introduced from the high-temperature air inlet hole 11a, so that the high-temperature air is sprayed out through the high-temperature air outlet hole 11b to enter the interior of the sterilizing box 1, and then all the ultraviolet lamps 71 are turned on;
S2: the culture dishes to be disinfected by opening the left arc-shaped door plate 21 are placed in the disinfection frame assembly, the left arc-shaped door plate 21 is closed, when the left rotating wheel 41 is controlled to drive the next disinfection frame assembly to be opposite to the left arc-shaped door plate 21, the rotation of the left rotating wheel 41 is stopped, the left arc-shaped door plate 21 is opened, then the culture dishes are manually installed in the next disinfection frame assembly, and the sequential operation is carried out until all the culture dishes are completely fed;
s3: the left rotating wheel 41 is controlled to drive the sterilizing rack assembly to the outer side of the first rotating wheel 51;
s4: the first rotating wheel 51 drives the sterilizing rack assembly to rotate, and the high-temperature sterilizing assembly sprays high-temperature air on the surface of the culture dish to sterilize the culture dish at high temperature;
S5: when the culture dish is sterilized at high temperature, the first rotating wheel 51 is controlled to drive the sterilizing rack assembly to be transported to the outer side of the second rotating wheel 52;
S6: the second rotating wheel 52 drives the sterilizing rack assembly to rotate, and ultraviolet rays emitted by the ultraviolet sterilizing assembly irradiate the surface of the culture dish to sterilize the culture dish by ultraviolet rays;
S7: after the culture dish completes ultraviolet sterilization, the second rotating wheel 52 is controlled to drive the sterilizing rack assembly to move to the outer side of the right rotating wheel 42;
S8: when the sterilizing rack assembly rotates to be aligned with the right arc-shaped door plate 22, the right arc-shaped door plate 22 is opened to take down the sterilized culture dish, and discharging is completed.
In the above S2 of the present embodiment, as shown in fig. 1, when the left arc door panel 21 is opened, the sterilization area in the sterilization box 1 is not communicated with the outside due to the left radial plate 411. The culture dish is installed in the mounting hole 311 of the mounting plate 31, the pressing block 312 is pushed by the elastic sheet 313 to fix the culture dish, after one mounting plate 31 is fully installed, the left arc-shaped door plate 21 is closed, the first motor 8 is started to drive the left rotating wheel 41 to rotate anticlockwise through the left rotating shaft 4a, the shaft lever 34 always moves in the left rail 171 due to the fact that the first shifting plate 911 is positioned in the position shown in fig. 1 at this time, when the next mounting plate 31 is opposite to the left arc-shaped door plate 21, the first motor 8 stops rotating, the left arc-shaped door plate 21 is opened, and then the culture dish is manually installed on the next mounting plate 31.
In the above S3 of the present embodiment, when the sterilizing rack assembly is controlled to move from the left rotating wheel 41 to the first rotating wheel 51, the first motor 8 is started to rotate counterclockwise, the left gear 61 is driven to rotate, and the left gear 61, the first gear 62, the second gear 63 and the right gear 64 are meshed, so that the first gear 62 and the right gear 64 rotate clockwise, and the second gear 63 rotates counterclockwise; thereby drive the first rotating wheel 51 to rotate clockwise, because the axostylus axostyle 34 slides along the left orbit 171 under the effect of left rotating wheel 41, when the mounting panel 31 of not installing the culture dish moves to the position that left-hand turning groove 413 and first groove 511 are aligned, first electric putter 913 keeps completely contracting and keeping still, first motor 8 drives axostylus axostyle 34 and continues to rotate, axostylus axostyle 34 gets into left orbit 171 rotation, when the mounting panel 31 of installing the culture dish moves to the position that left-hand turning groove 413 and first groove 511 are aligned, first motor 8 drives axostylus axostyle 34 rotation, when left-hand radial plate 411 is not in hindering first putting through plate 911 rotation, first electric putter 913 stretches completely, drive first connecting rod 912 rotation, because first connecting rod 912 and first putting through plate 911 fixed connection, and then make first putting through plate 911 rotation, push axostylus axostyle 34 gets into first orbit 172, follow first rotating wheel 51 rotation, after axostylus axostyle 34 gets into first orbit 172, first electric putter 913 is controlled to reset, avoid touching 411 with left-hand radial plate 923, when second electric putter 923 keeps completely contracting and making first groove 172 rotate along first groove 172, make first groove 172 rotate along the position that first groove 511 keeps on the first groove and first rotating groove 51, when the first rotating through the first groove is kept in the same.
In S4 of the embodiment, when the shaft 34 rotates along the first track 172, the third gear 33 and the first incomplete gear 13 are meshed, and the third gear 33 can be driven to rotate 180 ° so as to drive the mounting plate 31 to turn over 180 °, thereby completing the turning over of the culture dish on the mounting plate 31, and the high-temperature air in the high-temperature air outlet 11b is sprayed on the surface of the culture dish, so that the culture dish can be sterilized at high temperature.
In S5 of the present embodiment, when the sterilizing rack assembly is controlled to be moved from the first rotating wheel 51 to the second rotating wheel 52, the second electric push rod 923 is controlled to be fully extended to drive the second shifting plate 921 to swing, so that the shaft lever 34 enters the second track 173, the mounting plate 31 is enabled to rotate along with the second rotating wheel 52, when the shaft lever 34 moves to a position where the right rotating groove 423 and the second rotating groove 521 are aligned, the third electric push rod 933 is controlled to be contracted, when the right radial plate 421 is not in the way of preventing the rotation of the third shifting plate 931, the third electric push rod 933 is controlled to be fully extended, so that the third shifting plate 931 is enabled to rotate, the shaft lever 34 is pushed to enter the second track 173, and the third electric push rod 933 drives the third shifting plate 931 to reset to avoid touching the right radial plate 173 after the shaft lever 34 enters the second track 173, and at this time, the second shifting plate 921 is enabled to push the shaft lever 34 to rotate along the second track 173 due to the fact that the second electric push rod 421 remains fully extended.
In S6 of the embodiment, when the shaft 34 rotates along the second track 173 and the third gear 33 is meshed with the second incomplete gear 15, the third gear 33 can be driven to rotate 180 ° and the mounting plate 31 can be driven to turn over 180 °, so that the dish on the mounting plate 31 is turned over, and the ultraviolet light emitted by the ultraviolet lamp 71 irradiates the surface of the dish.
In S7 of the present embodiment, when the sterilizing rack assembly is controlled to move from the second rotating wheel 52 to the right rotating wheel 42, the third electric push rod 933 is controlled to be completely contracted when the shaft lever 34 is moved to the position where the right rotating groove 423 and the second rotating groove 521 are aligned, so that the third dial 931 pushes the shaft lever 34 into the right rail 174 to rotate following the right rotating wheel 42.
In addition, when a culture dish is in the device and a new culture dish is added into the device to be disinfected together, the first motor 8 drives the left rotating wheel 41 to rotate, so that the unused mounting plate 31 which rotates along with the left rotating wheel 41 rotates to a position opposite to the left arc-shaped door plate 21, then the left arc-shaped door plate 21 is opened, the disinfection area in the disinfection box 1 is not communicated with the outside due to the action of the left radial plate 411, then the mounting plate 31 is fed, after the feeding is finished, the first motor 8 is started to drive the left rotating wheel 41 to drive the mounting plate 31 to rotate, and the newly-mounted mounting plate 31 enters the first track 172 to be disinfected together with the original mounting plate 31 under the condition that the first shifting plate 911 is selected; through the selection of the second dial 921, the mounting plate 31 can be selected to enter the second track 173 or remain in the first track 172, when the original mounting plate 31 is disinfected, the mounting plate 31 with the disinfection end is controlled to enter the right track 174 through the selection of the third dial 931, when the mounting plate 31 in the right track 174 moves to be aligned with the right arc door plate 22, the first motor 8 stops, the right arc door plate 22 can be opened, the disinfection area is separated from the outside due to the action of the isolation right radial plate 421, and then the disinfected culture dish can be manually taken down to finish midway blanking.
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the scope of the invention.
Claims (8)
1. The stem cell culture dish disinfection system is characterized by comprising a disinfection frame assembly and a disinfection box with an opening at the upper end, wherein the disinfection box comprises a main box body in the middle, a left semicircular box body arranged at the left end of the main box body and a right semicircular box body arranged at the right end of the main box body, the left side of the left semicircular box body is provided with a left arc-shaped door plate, and the right side of the right semicircular box body is provided with a right arc-shaped door plate; the bottom of the disinfection box is provided with a left turning hole, a first turning hole, a second turning hole and a right turning hole in sequence from left to right; a top cover is fixedly arranged at the upper end of the disinfection box, and a bottom plate which is blocked on the left turning hole, the first turning hole, the second turning hole and the right turning hole is fixedly arranged at the lower end of the disinfection box; a left rotating wheel positioned in the left rotating hole is rotationally connected to the center of the left semicircular box body in the sterilizing box, and a right rotating wheel positioned in the right rotating hole is rotationally connected to the center of the right semicircular box body in the sterilizing box; the right side of the left rotating wheel in the top cover is provided with a high-temperature disinfection assembly, and the left side of the right rotating wheel is provided with an ultraviolet disinfection assembly; the first rotating wheel positioned in the first rotating hole is rotationally connected to the bottom plate under the high-temperature sterilizing component, and the second rotating wheel positioned in the second rotating hole is arranged under the ultraviolet sterilizing component; the sterilizing rack assembly is positioned in the sterilizing box and used for placing the culture dish, and under the action of the left rotating wheel, the first rotating wheel, the second rotating wheel and the right rotating wheel, the sterilizing rack assembly drives the culture dish to sequentially pass through the high-temperature sterilizing assembly and the ultraviolet sterilizing assembly;
A left rotating shaft at the center of the left rotating hole is rotationally connected between the top cover and the bottom plate, the left rotating wheel is fixedly arranged on the left rotating shaft, a plurality of left radial plates are uniformly arranged on the outer side of the left rotating wheel along the circumferential direction at intervals, and when the left radial plates rotate around the left rotating shaft, the left radial plates are abutted against the inner side wall of the left semicircular box body; a right rotating shaft at the center of the right rotating hole is rotationally connected between the top cover and the bottom plate, the right rotating wheel is fixedly arranged on the right rotating shaft, a plurality of right radial plates are uniformly arranged on the outer side of the right rotating wheel along the circumferential direction at intervals, and when the right radial plates rotate around the right rotating shaft, the right radial plates are abutted against the inner side wall of the right semicircular box body;
A left track is formed between the left rotating hole and the left rotating wheel, a first track is formed between the first rotating hole and the first rotating wheel, a second track is formed between the second rotating hole and the second rotating wheel, and a right track is formed between the right rotating hole and the right rotating wheel; the disinfection frame assembly comprises a shaft rod, a left rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the left rotating wheel, a first rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the first rotating wheel, a second rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the second rotating wheel, and a right rotating groove for the shaft rod to extend in is formed in the outer circumferential side of the right rotating wheel; the upper end of the shaft lever is provided with a limiting convex edge which is positioned above the first rotating wheel and the second rotating wheel and is used for being clamped into the outer sides of the left rotating wheel and the right rotating wheel, the upper end of the limiting convex edge is provided with an upward extending mounting plate, and a plurality of mounting holes for placing culture dishes are formed in the mounting plate; a third gear is fixedly arranged at the upper end of the mounting plate;
The connecting part of the left rotating hole and the first rotating hole in the bottom plate is provided with a first rotating rod, the upper end of the first rotating rod is fixedly provided with a first shifting plate, the lower end of the first rotating rod is fixedly provided with a first connecting rod, the lower end of the bottom plate is hinged with a first electric push rod, and the telescopic end of the first electric push rod is hinged with the first connecting rod; when the first electric push rod is completely contracted, the left rotating wheel drives the shaft rod to move in the left track, and when the first electric push rod is completely extended, the first shifting plate guides the shaft rod to move in the first track from the left track;
A second rotating rod is arranged at the joint of the first rotating hole and the second rotating hole in the bottom plate, a second shifting plate is fixedly arranged at the upper end of the second rotating rod, a second connecting rod is fixedly arranged at the lower end of the second rotating rod, a second electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the second electric push rod is hinged to the second connecting rod; when the second electric push rod is completely contracted, the first rotating wheel drives the shaft rod to move in the first track, and when the second electric push rod is completely extended, the second shifting plate guides the shaft rod to move from the first track into the second track;
A third rotating rod is arranged at the joint of the second rotating hole and the right rotating hole in the bottom plate, a third shifting plate is fixedly arranged at the upper end of the third rotating rod, a third connecting rod is fixedly arranged at the lower end of the third rotating rod, a third electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the third electric push rod is hinged to the third connecting rod; when the third electric push rod is completely extended, the second rotating wheel drives the shaft rod to move in the second track, and when the first electric push rod is completely contracted, the third shifting plate guides the shaft rod to move in the right track from the second track.
2. The stem cell culture dish sterilization system according to claim 1, wherein a plurality of left avoidance grooves into which the left radial plate extends are uniformly arranged on the circumferential outer side of the first rotating wheel at intervals in the circumferential direction, and a plurality of right avoidance grooves into which the right radial plate extends are uniformly arranged on the circumferential outer side of the second rotating wheel at intervals in the circumferential direction.
3. The stem cell culture dish sterilization system according to claim 1, wherein the bottom plate is rotatably connected with a first rotating shaft at the center of the first rotating hole, and is rotatably connected with a second rotating shaft at the center of the second rotating hole, the first rotating wheel is fixedly installed on the first rotating shaft, and the second rotating wheel is fixedly installed on the second rotating shaft.
4. The stem cell culture dish sterilization system according to claim 3, wherein the lower end of the left rotating shaft extends out of the bottom plate and is fixedly provided with a left gear, the lower end of the first rotating shaft extends out of the bottom plate and is fixedly provided with a first gear meshed with the left gear, the lower end of the second rotating shaft extends out of the bottom plate and is fixedly provided with a second gear meshed with the first gear, and the lower end of the right rotating shaft extends out of the bottom plate and is fixedly provided with a right gear meshed with the second gear; the lower extreme of bottom plate is equipped with the support frame, fixedly on the support frame be equipped with be used for driving left pivot pivoted first motor.
5. The stem cell culture dish sterilization system according to claim 1, wherein the side wall of the mounting hole is provided with a mounting groove, a pressing block is hinged in the mounting groove, and a spring plate for forcing the pressing block to extend into the mounting hole; the briquetting is used for compressing the culture dish in the mounting hole.
6. The stem cell culture dish sterilization system according to claim 1, wherein the high-temperature sterilization assembly comprises a convex column which is downwards arranged on the top cover along the vertical direction, a vertically arranged high-temperature air inlet hole is arranged in the convex column, and a high-temperature air outlet hole communicated with the high-temperature air inlet hole is arranged on the outer side of the circumference of the convex column; the outside symmetry of projection is equipped with first incomplete gear, first incomplete gear is used for with third gear meshing drive axostylus axostyle rotation.
7. The stem cell culture dish sterilization system according to claim 1, wherein the ultraviolet sterilization assembly comprises a convex plate arranged on the top cover downwards along the vertical direction, and a plurality of ultraviolet lamps are fixedly arranged on the outer side of the convex plate; the outer side of the convex plate is fixedly provided with a second incomplete gear, and the second incomplete gear is used for being meshed with a third gear to drive the shaft rod to rotate.
8. A method of sterilizing a culture dish using the stem cell culture dish sterilization system of claim 1, comprising the steps of:
S1: introducing high-temperature air from the high-temperature air inlet hole, enabling the high-temperature air to be sprayed out through the high-temperature air outlet hole and enter the interior of the sterilizing box, and then opening all ultraviolet lamps;
S2: placing a culture dish to be sterilized by opening the left arc-shaped door plate in a sterilizing rack assembly, closing the left arc-shaped door plate, stopping the rotation of the left rotating wheel when the left rotating wheel is controlled to drive the next sterilizing rack assembly to face the left arc-shaped door plate, opening the left arc-shaped door plate, then manually installing the culture dish in the next sterilizing rack assembly, and sequentially operating until all culture dishes are completely fed;
s3: controlling the left rotating wheel to drive the sterilizing frame assembly to move to the outer side of the first rotating wheel;
S4: the first rotating wheel drives the sterilizing rack assembly to rotate, and the high-temperature sterilizing assembly sprays high-temperature air on the surface of the culture dish to sterilize the culture dish at high temperature;
S5: when the culture dish is sterilized at high temperature, the first rotating wheel is controlled to drive the sterilizing rack assembly to be transported to the outer side of the second rotating wheel;
s6: the second rotating wheel drives the sterilizing rack assembly to rotate, and ultraviolet rays emitted by the ultraviolet sterilizing assembly irradiate the surface of the culture dish to sterilize the culture dish by ultraviolet rays;
S7: after ultraviolet sterilization of the culture dish is completed, controlling the second rotating wheel to drive the sterilizing rack assembly to move to the outer side of the right rotating wheel;
S8: when the sterilizing rack assembly rotates to be aligned with the right arc-shaped door plate, the right arc-shaped door plate is opened to take down the sterilized culture dish, and discharging is completed.
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