CN114949283A - Stem cell culture dish disinfection system and disinfection method - Google Patents
Stem cell culture dish disinfection system and disinfection method Download PDFInfo
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- CN114949283A CN114949283A CN202210829653.5A CN202210829653A CN114949283A CN 114949283 A CN114949283 A CN 114949283A CN 202210829653 A CN202210829653 A CN 202210829653A CN 114949283 A CN114949283 A CN 114949283A
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 148
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 25
- 238000004113 cell culture Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 9
- 230000001954 sterilising effect Effects 0.000 claims abstract description 25
- 230000009471 action Effects 0.000 claims abstract description 9
- 230000000249 desinfective effect Effects 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000007306 turnover Effects 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultra-violet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
- A61L2/06—Hot gas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/24—Apparatus using programmed or automatic operation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/12—Apparatus for isolating biocidal substances from the environment
- A61L2202/121—Sealings, e.g. doors, covers, valves, sluices
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/10—Apparatus features
- A61L2202/14—Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a stem cell culture dish disinfection system and a disinfection method, wherein the stem cell culture dish disinfection system comprises a disinfection frame assembly and a disinfection box with an opening at the upper end, wherein a left arc-shaped door plate is arranged on the left side of the disinfection box, and a right arc-shaped door plate is arranged on the right side of the disinfection box; a left rotating wheel and a right rotating wheel are rotationally connected in the sterilizing box; a high-temperature disinfection component and an ultraviolet disinfection component are arranged in the top cover; a first rotating wheel positioned in the first rotating hole is rotatably connected to the bottom plate under the high-temperature disinfection component, and a second rotating wheel positioned in the second rotating hole is arranged under the ultraviolet disinfection component; the sterilizing rack assembly is positioned in the sterilizing box and used for placing the culture dish, and under the action of the left rotating wheel, the first rotating wheel, the second rotating wheel and the right rotating wheel, the sterilizing rack assembly drives the culture dish to sequentially pass through the high-temperature sterilizing assembly and the ultraviolet sterilizing assembly; this stem cell culture dish disinfection system is simple structure not only, can realize the disinfection of culture dish by the high efficiency moreover.
Description
Technical Field
The invention relates to the technical field of stem cells, in particular to a stem cell culture dish disinfection system and a disinfection method.
Background
The culture dish is a laboratory vessel for culturing microorganisms or cells, generally comprises a bottom and a cover of a disc, is mainly made of plastic and glass, and is beneficial to observing and operating the microorganisms due to the transparency of the culture dish. The culture dish is mainly used for microorganism culture, iron wall culture of animal cells, laboratory inoculation, streaking, bacterium separation and the like, is applied to stem cell experiments for decades, and is indispensable culture equipment in the stem cell experiments. In prior art, do not have external disturbance in order to guarantee the experimental result, need disinfect the culture dish before the culture dish uses, reduce the influence of harmful bacterium to the stem cell experiment, but current equipment when carrying out the culture dish disinfection, the following problem generally can appear:
1. when the existing equipment is used for disinfecting the culture dish, the disinfection equipment must be completely closed, so that the culture dish cannot be added midway for disinfection in the disinfection process;
2. when the existing equipment is used for disinfecting the culture dish, the culture dish is generally disinfected by irradiation of the ultraviolet lamp, but because the irradiation of the ultraviolet lamp to the culture dish is uneven, illumination dead angles exist, the disinfection effect of the culture dish is not ideal, and the disinfection is not thorough.
Disclosure of Invention
The invention aims to provide a stem cell culture dish disinfection system which is simple in structure and capable of efficiently disinfecting a culture dish.
In order to achieve the purpose, the invention provides the following technical scheme: a stem cell culture dish disinfection system comprises a disinfection frame assembly and a disinfection box with an opening at the upper end, wherein the disinfection box comprises a main box body in the middle, a left semicircular box body arranged at the left end of the main box body and a right semicircular box body arranged at the right end of the main box body; the bottom of the disinfection box is sequentially provided with a left rotating hole, a first rotating hole, a second rotating hole and a right rotating hole from left to right; the upper end of the disinfection box is fixedly provided with a top cover, and the lower end of the disinfection box is fixedly provided with a bottom plate which is plugged on the left rotating hole, the first rotating hole, the second rotating hole and the right rotating hole; a left rotating wheel positioned in the left rotating hole is rotatably connected at the circle center of the left semicircular box body in the disinfection box, and a right rotating wheel positioned in the right rotating hole is rotatably connected at the circle center of the right semicircular box body in the disinfection box; a high-temperature disinfection component is arranged on the right side of the left rotating wheel in the top cover, and an ultraviolet disinfection component is arranged on the left side of the right rotating wheel; a first rotating wheel positioned in the first rotating hole is rotatably connected to the bottom plate under the high-temperature disinfection component, and a second rotating wheel positioned in the second rotating hole is arranged under the ultraviolet disinfection component; the disinfection frame assembly is located the disinfect box and is used for placing the culture dish, and under the action of left runner, first runner, second runner, right runner, the disinfection frame assembly drives the culture dish and passes through high temperature disinfection subassembly and ultraviolet ray disinfection subassembly in proper order.
Furthermore, a left rotating shaft at the center of the left rotating hole is rotatably connected between the top cover and the bottom plate, the left rotating wheel is fixedly installed on the left rotating shaft, a plurality of left radial plates are uniformly arranged on the outer side of the left rotating wheel at intervals along the circumferential direction, and when the left radial plates rotate around the left rotating shaft, the left radial plates are abutted against the inner side wall of the left semicircular box body; rotate between top cap and the bottom plate and be connected with the right turn axle that the hole centre of a circle was located to the position right side, right side runner fixed mounting is in the pivot of right side, the outside of right side runner is equipped with a plurality of right radial plates along the circumference interval uniformly, when right radial plate rotated around the pivot of right side, right radial plate contradicted on the inside wall of right semicircle box.
Further, the circumference outside of first runner is equipped with a plurality of left sides that supply left radial plate to stretch into along the circumference interval uniformly and dodges the groove, the circumference outside of second runner is equipped with a plurality of right sides that supply right radial plate to stretch into along the circumference interval uniformly and dodges the groove.
Furthermore, a first rotating shaft is rotatably connected to the center of the first rotating hole on the bottom plate, a second rotating shaft is rotatably connected to the center of the second rotating hole, the first rotating wheel is fixedly mounted on the first rotating shaft, and the second rotating wheel is fixedly mounted on the second rotating shaft.
Furthermore, the lower end of the left rotating shaft extends out of the bottom plate and is fixedly provided with a left gear, the lower end of the first rotating shaft extends out of the bottom plate and is fixedly provided with a first gear meshed with the left gear, the lower end of the second rotating shaft extends out of the bottom plate and is fixedly provided with a second gear meshed with the first gear, and the lower end of the right rotating shaft extends out of the bottom plate and is fixedly provided with a right gear meshed with the second gear; the lower extreme of bottom plate is equipped with the support frame, it is used for driving left pivot pivoted first motor to fix on the support frame.
Further, a left track is formed between the left rotating hole and the left rotating wheel, a first track is formed between the first rotating hole and the first rotating wheel, a second track is formed between the second rotating hole and the second rotating wheel, and a right track is formed between the right rotating hole and the right rotating wheel; the disinfection frame assembly comprises a shaft lever, a left rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the left rotating wheel, a first rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the first rotating wheel, a second rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the second rotating wheel, and a right rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the right rotating wheel; the upper end of the shaft lever is provided with a limiting convex edge which is positioned above the first rotating wheel and the second rotating wheel and is used for being clamped into the outer sides of the left rotating wheel and the right rotating wheel, the upper end of the limiting convex edge is provided with an upward extending mounting plate, and a plurality of mounting holes for placing culture dishes are formed in the mounting plate; a third gear is fixedly arranged at the upper end of the mounting plate;
a first rotating rod is arranged at the joint of the left rotating hole and the first rotating hole in the bottom plate, a first shifting plate is fixedly arranged at the upper end of the first rotating rod, a first connecting rod is fixedly arranged at the lower end of the first rotating rod, a first electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the first electric push rod is hinged to the first connecting rod; when the first electric push rod is completely contracted, the left rotating wheel drives the shaft lever to move in the left track, and when the first electric push rod is completely extended out, the first shifting plate guides the shaft lever to enter the first track from the left track to move;
a second rotating rod is arranged at the joint of the first rotating hole and the second rotating hole in the bottom plate, a second shifting plate is fixedly arranged at the upper end of the second rotating rod, a second connecting rod is fixedly arranged at the lower end of the second rotating rod, a second electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the second electric push rod is hinged to the second connecting rod; when the second electric push rod is completely contracted, the first rotating wheel drives the shaft lever to move in the first track, and when the second electric push rod is completely extended out, the second shifting plate guides the shaft lever to enter the second track from the first track to move;
a third rotating rod is arranged at the joint of the second rotating hole and the right rotating hole in the bottom plate, a third shifting plate is fixedly arranged at the upper end of the third rotating rod, a third connecting rod is fixedly arranged at the lower end of the third rotating rod, a third electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the third electric push rod is hinged to the third connecting rod; when third electric putter stretches out completely, the second runner drives the axostylus axostyle and moves in the second track, and when first electric putter contracts completely, the third is dialled the board and is guided the axostylus axostyle and gets into right track internal motion by the second track.
Furthermore, the side wall of the mounting hole is provided with a mounting groove, a pressing block and a spring plate for forcing the pressing block to extend into the mounting hole are hinged in the mounting groove; the pressing block is used for pressing the culture dish in the mounting hole.
Furthermore, the high-temperature disinfection component comprises a convex column which is downwards arranged on the top cover along the vertical direction, a high-temperature air inlet hole which is vertically arranged is arranged in the convex column, and a high-temperature air outlet hole which is communicated with the high-temperature air inlet hole is arranged on the outer side of the circumference of the convex column; the outside symmetry of projection is equipped with first incomplete gear, first incomplete gear is used for driving the axostylus axostyle rotation with the third gear engagement.
Furthermore, the ultraviolet disinfection component comprises a convex plate which is downwards arranged on the top cover along the vertical direction, and a plurality of ultraviolet lamps are fixedly arranged on the outer side of the convex plate; the outside of flange is fixed and is equipped with the incomplete gear of second, the incomplete gear of second is used for driving the axostylus axostyle rotation with the third gear engagement.
The invention also discloses a method for disinfecting the culture dish by using the stem cell culture dish disinfection system, which comprises the following steps:
s1: introducing high-temperature air from the high-temperature air inlet hole, spraying the high-temperature air into the disinfection box through the high-temperature air outlet hole, and then opening all the ultraviolet lamps;
s2: placing the culture dishes to be disinfected after the left arched door panel is opened in the disinfection frame assembly, closing the left arched door panel, controlling the left rotating wheel to drive the next disinfection frame assembly to be opposite to the left arched door panel, stopping the rotation of the left rotating wheel, opening the left arched door panel, manually installing the culture dishes in the next disinfection frame assembly, and sequentially operating until all the culture dishes are completely loaded;
s3: controlling the left rotating wheel to drive the disinfecting frame assembly to be conveyed to the outer side of the first rotating wheel;
s4: the first rotating wheel drives the disinfection frame assembly to rotate, and the high-temperature disinfection assembly sprays high-temperature air on the surface of the culture dish to disinfect the culture dish at high temperature;
s5: after the culture dish is disinfected at high temperature, the first rotating wheel is controlled to drive the disinfection frame assembly to be conveyed to the outer side of the second rotating wheel;
s6: the second rotating wheel drives the disinfection frame assembly to rotate, and ultraviolet rays emitted by the ultraviolet disinfection assembly irradiate the surface of the culture dish to disinfect the culture dish by the ultraviolet rays;
s7: after the culture dish finishes ultraviolet disinfection, controlling the second rotating wheel to drive the disinfection frame assembly to be transported to the outer side of the right rotating wheel;
s8: when disinfecting frame subassembly rotated to and aligns with right arc door plant, opened right arc door plant and take off the culture dish after the disinfection, accomplished the unloading.
Advantageous effects
Compared with the prior art, the technical scheme of the invention has the following advantages:
1. the left rotating wheel with the left radial plate is arranged at the left arc-shaped door plate, the right rotating wheel with the right radial plate is arranged at the right arc-shaped door plate, so that when a culture dish needing to be disinfected is fed or sterilized is discharged, the disinfection part of the equipment can be sealed, the disinfection part is not in contact with the outside, the disinfection of the culture dish is not delayed during feeding and discharging, the culture dish can be still added into the equipment during the disinfection process, and meanwhile, the disinfected culture dish can be discharged;
2. the left rotating wheel, the first rotating wheel, the second rotating wheel and the right rotating wheel are arranged in the device, and the first shifting plate, the second shifting plate and the third shifting plate are arranged at the same time, so that the shaft rod can be selected to enter the left rail, the second rail or the right rail, and then the disinfection cycle is automatically carried out, and the device is convenient and fast;
3. when the mounting plate is disinfected in the high-temperature disinfection area and the ultraviolet disinfection area through the first incomplete gear and the second incomplete gear, the first incomplete gear and the second incomplete gear are respectively matched with a third gear on the mounting plate to drive the mounting plate to rotate, and further culture dish turnover on the mounting plate is controlled, so that disinfection dead corners are reduced, and disinfection quality is improved;
4. through setting up high temperature venthole and ultraviolet lamp for be provided with high temperature disinfection district and ultraviolet ray disinfection district in the disinfect box, thereby carry out ultraviolet sterilization after the high temperature disinfection to the culture dish of installing on the mounting panel, the disinfection is many times promoted disinfection effect.
Drawings
FIG. 1 is a cross-sectional view of the present invention;
FIG. 2 is a sectional view taken along line A-A of FIG. 1 in accordance with the present invention;
FIG. 3 is a sectional view taken along line B-B of FIG. 1 in accordance with the present invention;
FIG. 4 is a cross-sectional view taken along line C-C of FIG. 1 in accordance with the present invention;
FIG. 5 is a cross-sectional view taken along line D-D of FIG. 1 in accordance with the present invention;
FIG. 6 is a sectional view taken along line E-E of FIG. 1 in accordance with the present invention.
Detailed Description
Referring to fig. 1-6, a stem cell culture dish disinfection system comprises a disinfection frame assembly and a disinfection box 1 with an opening at the upper end, wherein the disinfection box 1 comprises a main box body in the middle, a left semicircular box body arranged at the left end of the main box body and a right semicircular box body arranged at the right end of the main box body, a left arc-shaped door panel 21 is arranged at the left side of the left semicircular box body, and a right arc-shaped door panel 22 is arranged at the right side of the right semicircular box body; the bottom of the disinfection box 1 is sequentially provided with a left rotating hole 1c, a first rotating hole 1d, a second rotating hole 1e and a right rotating hole 1f from left to right; a top cover 1a is fixedly arranged at the upper end of the disinfection box 1, and a bottom plate 1b which is blocked on a left rotating hole 1c, a first rotating hole 1d, a second rotating hole 1e and a right rotating hole 1f is fixedly arranged at the lower end of the disinfection box; a left rotating wheel 41 positioned in the left rotating hole 1c is rotatably connected at the circle center of the left semicircular box body in the disinfection box 1, and a right rotating wheel 42 positioned in the right rotating hole 1f is rotatably connected at the circle center of the right semicircular box body; a high-temperature disinfection component is arranged on the right side of the left rotating wheel 41 in the top cover 1a, and an ultraviolet disinfection component is arranged on the left side of the right rotating wheel 42; a first rotating wheel 51 positioned in the first rotating hole 1d and a second rotating wheel 52 positioned in the second rotating hole 1e are rotatably connected to the bottom plate 1b under the high-temperature disinfection component; the disinfection frame assembly is located the disinfect box 1 and is used for placing the culture dish, and under the action of the left rotating wheel 41, the first rotating wheel 51, the second rotating wheel 52 and the right rotating wheel 42, the disinfection frame assembly drives the culture dish to sequentially pass through the high-temperature disinfection assembly and the ultraviolet disinfection assembly.
A left rotating shaft 4a positioned at the circle center of the left rotating hole 1c is rotatably connected between the top cover 1a and the bottom plate 1b, the left rotating wheel 41 is fixedly installed on the left rotating shaft 4a, a plurality of left radial plates 411 are uniformly arranged on the outer side of the left rotating wheel 41 at intervals along the circumferential direction, and when the left radial plates 411 rotate around the left rotating shaft 4a, the left radial plates 411 are abutted against the inner side wall of the left semicircular box body; rotate between top cap 1a and the bottom plate 1b and be connected with the right pivot 4b that the position turned right hole 1f centre of a circle department, right runner 42 fixed mounting is on right pivot 4b, the outside of right runner 42 is equipped with a plurality of right radial plates 421 along the circumference interval uniformly, when right radial plate 421 rotates around right pivot 4b, right radial plate 421 contradicts on the inside wall of right semicircle box.
The circumference outside of first runner 51 is equipped with a plurality of left sides that supply left radial plate 411 to stretch into along the circumference interval uniformly and dodges groove 5a, the circumference outside of second runner 52 is equipped with a plurality of right sides that supply right radial plate 421 to stretch into along the circumference interval uniformly and dodges groove 5 b. The bottom plate 1b is rotatably connected with a first rotating shaft 5c at the circle center of the first rotating hole 1d, and is rotatably connected with a second rotating shaft 5d at the circle center of the second rotating hole 1e, the first rotating wheel 51 is fixedly installed on the first rotating shaft 5c, and the second rotating wheel 52 is fixedly installed on the second rotating shaft 5 d. The lower end of the left rotating shaft 4a extends out of the bottom plate 1b and is fixedly provided with a left gear 61, the lower end of the first rotating shaft 5c extends out of the bottom plate 1b and is fixedly provided with a first gear 62 meshed with the left gear 61, the lower end of the second rotating shaft 5d extends out of the bottom plate 1b and is fixedly provided with a second gear 63 meshed with the first gear 62, and the lower end of the right rotating shaft 4b extends out of the bottom plate 1b and is fixedly provided with a right gear 64 meshed with the second gear 63; the lower extreme of bottom plate 1b is equipped with support frame 1h, fixed being equipped with on support frame 1h is used for driving left pivot 4a pivoted first motor 8.
A left track 171 is formed between the left rotating hole 1c and the left rotating wheel 41, a first track 172 is formed between the first rotating hole 1d and the first rotating wheel 51, a second track 173 is formed between the second rotating hole 1e and the second rotating wheel 52, and a right track 174 is formed between the right rotating hole 1f and the right rotating wheel 42; the disinfection frame assembly comprises a shaft rod 34, a left rotary groove 413 for the shaft rod 34 to extend into is arranged on the outer side of the circumference of the left rotary wheel 41, a first rotary groove 511 for the shaft rod 34 to extend into is arranged on the outer side of the circumference of the first rotary wheel 51, a second rotary groove 521 for the shaft rod 34 to extend into is arranged on the outer side of the circumference of the second rotary wheel 52, and a right rotary groove 423 for the shaft rod 34 to extend into is arranged on the outer side of the circumference of the right rotary wheel 42; the upper end of the shaft rod 34 is provided with a limiting convex edge 301 which is positioned above the first rotating wheel 51 and the second rotating wheel 52 and is used for being clamped into the outer sides of the left rotating wheel 41 and the right rotating wheel 42, the upper end of the limiting convex edge 301 is provided with an upward extending mounting plate 31, and a plurality of mounting holes 311 for placing culture dishes are arranged in the mounting plate 31; a third gear 33 is fixedly arranged at the upper end of the mounting plate 31; the side wall of the mounting hole 311 is provided with a mounting groove 3a, a pressing block 312 and an elastic sheet 313 for forcing the pressing block 312 to extend into the mounting hole 311 are hinged in the mounting groove 3 a; the pressing block 312 is used for pressing the culture dish in the mounting hole 311.
A first rotating rod 9a is arranged at the joint of the left rotating hole 1c and the first rotating hole 1d in the bottom plate 1b, a first shifting plate 911 is fixedly arranged at the upper end of the first rotating rod 9a, a first connecting rod 912 is fixedly arranged at the lower end of the first rotating rod 9a, a first electric push rod 913 is hinged to the lower end of the bottom plate 1b, and the telescopic end of the first electric push rod 913 is hinged to the first connecting rod 912; when the first electric push rod 913 is fully retracted, the left wheel 41 drives the shaft 34 to move in the left rail 171, and when the first electric push rod 913 is fully extended, the first pulling plate 911 guides the shaft 34 to move from the left rail 171 into the first rail 172; a second rotating rod 9b is arranged at the joint of the first rotating hole 1d and the second rotating hole 1e in the bottom plate 1b, a second shifting plate 921 is fixedly arranged at the upper end of the second rotating rod 9b, a second connecting rod 922 is fixedly arranged at the lower end of the second rotating rod 9b, a second electric push rod 923 is hinged to the lower end of the bottom plate 1b, and the telescopic end of the second electric push rod 923 is hinged to the second connecting rod 922; when the second electric push rod 923 is completely retracted, the first rotating wheel 51 drives the shaft rod 34 to move in the first track 172, and when the second electric push rod 923 is completely extended, the second shifting plate 921 guides the shaft rod 34 to move from the first track 172 into the second track 173; a third rotating rod 9c is arranged at the joint of the second rotating hole 1e and the right rotating hole 1f in the bottom plate 1b, a third shifting plate 931 is fixedly arranged at the upper end of the third rotating rod 9c, a third connecting rod 932 is fixedly arranged at the lower end of the third rotating rod 9c, a third electric push rod 933 is hinged to the lower end of the bottom plate 1b, and the telescopic end of the third electric push rod 933 is hinged to the third connecting rod 932; when the third electric push rod 933 is fully extended, the second runner 52 drives the shaft 34 to move in the second rail 173, and when the first electric push rod 913 is fully retracted, the third dial 931 guides the shaft 34 to move from the second rail 173 into the right rail 174.
The high-temperature disinfection component comprises a convex column 11 which is downwards arranged on the top cover 1a along the vertical direction, a high-temperature air inlet 11a which is vertically arranged is arranged in the convex column 11, and a high-temperature air outlet 11b which is communicated with the high-temperature air inlet 11a is arranged on the outer side of the circumference of the convex column 11; the outer side of the convex column 11 is symmetrically provided with a first incomplete gear 13, and the first incomplete gear 13 is used for being meshed with a third gear 33 to drive a shaft 34 to rotate.
The ultraviolet disinfection component comprises a convex plate 7 which is arranged on the top cover 1a downwards along the vertical direction, and a plurality of ultraviolet lamps 71 are fixedly arranged on the outer side of the convex plate 7; a second incomplete gear 15 is fixedly arranged on the outer side of the convex plate 7, and the second incomplete gear 15 is used for being meshed with a third gear 33 and driving a shaft rod 34 to rotate.
The embodiment also provides a method for disinfecting a culture dish by using the stem cell culture dish disinfecting system, which comprises the following steps:
s1: introducing high-temperature air from the high-temperature air inlet hole 11a, spraying the high-temperature air into the disinfection box 1 through the high-temperature air outlet hole 11b, and then opening all the ultraviolet lamps 71;
s2: placing the culture dishes to be sterilized after the left arched door panel 21 is opened in the sterilizing shelf assembly, closing the left arched door panel 21, controlling the left rotating wheel 41 to drive the next sterilizing shelf assembly to be opposite to the left arched door panel 21, stopping the rotation of the left rotating wheel 41, opening the left arched door panel 21, manually installing the culture dishes in the next sterilizing shelf assembly, and sequentially operating until all the culture dishes are completely loaded;
s3: controlling the left rotating wheel 41 to drive the disinfection frame assembly to be conveyed to the outer side of the first rotating wheel 51;
s4: the first rotating wheel 51 drives the disinfection frame assembly to rotate, and the high-temperature disinfection assembly sprays high-temperature air on the surface of the culture dish to disinfect the culture dish at high temperature;
s5: after the culture dish is sterilized at high temperature, the first rotating wheel 51 is controlled to drive the sterilizing rack assembly to be conveyed to the outer side of the second rotating wheel 52;
s6: the second rotating wheel 52 drives the disinfection frame assembly to rotate, and ultraviolet rays emitted by the ultraviolet disinfection assembly irradiate the surface of the culture dish to disinfect the culture dish by ultraviolet rays;
s7: after the culture dish finishes ultraviolet disinfection, the second rotating wheel 52 is controlled to drive the disinfection frame assembly to be conveyed to the outer side of the right rotating wheel 42;
s8: when the disinfection rack assembly rotates to be aligned with the right arc-shaped door panel 22, the right arc-shaped door panel 22 is opened to take down the disinfected culture dish, and the blanking is completed.
In the above-mentioned S2 of the present embodiment, as shown in fig. 1, the initial position is that after the left door 21 is opened, the sterilizing area inside the sterilizing compartment 1 is not communicated with the outside due to the action of the left radial plate 411. Install the culture dish in the mounting hole 311 of mounting panel 31, it is fixed with the culture dish to promote briquetting 312 through shell fragment 313, after a mounting panel 31 ann was filled with, close left arched door board 21, start first motor 8 and drive left runner 41 anticlockwise rotation through left pivot 4a, because first group board 911 is in the position shown in figure 1 this moment, make axostylus axostyle 34 move in left track 171 always, when next mounting panel 31 is just to left arched door board 21, first motor 8 stall, open left arched door board 21, then the manual work is installed the culture dish on next mounting panel 31.
In the above S3 of this embodiment, when the disinfecting shelf assembly is controlled to move from the left wheel 41 to the first wheel 51, the first motor 8 is started to rotate counterclockwise to drive the left gear 61 to rotate, and since the left gear 61, the first gear 62, the second gear 63 and the right gear 64 are engaged, the first gear 62 and the right gear 64 rotate clockwise, and the second gear 63 rotates counterclockwise; thereby driving the first rotating wheel 51 to rotate clockwise, because the shaft lever 34 slides along the left track 171 under the action of the left rotating wheel 41, when the mounting plate 31 without the culture dish is moved to the position where the left rotating groove 413 is aligned with the first rotating groove 511, the first electric push rod 913 keeps completely retracted, the first electric motor 8 drives the shaft lever 34 to continue rotating, the shaft lever 34 enters the left track 171 to rotate, when the mounting plate 31 with the culture surface is moved to the position where the left rotating groove 413 is aligned with the first rotating groove 511, the first electric motor 8 drives the shaft lever 34 to rotate, when the left radial plate 411 does not hinder the rotation of the first shifting plate 911, the first electric push rod 913 completely extends to drive the first connecting rod 912 to rotate, because the first connecting rod 912 is fixedly connected with the first shifting plate 911, the first shifting plate 911 further rotates, the shaft lever 34 is pushed into the first track 172 to rotate along with the first rotating wheel 51, after the shaft lever 34 enters the first track 172, the first electric putter 913 controls the first board 911 to reset, avoiding touching with the left radial board 411, because the second electric putter 923 keeps contracting completely this moment, make the second dial board 921 promote the axostylus axostyle 34 to rotate along the first track 172, when the first runner 51 drives the axostylus axostyle 34 to come to the position that the left-hand groove 413 aligns with the first runner 511, through the effect of first electric putter 913, can make the axostylus axostyle 34 keep rotating in the first track 172 in the same way.
In the above-mentioned S4 of this embodiment, when shaft lever 34 rotates along first track 172, when third gear 33 and first incomplete gear 13 mesh, can drive third gear 33 and rotate 180 °, and then can drive mounting plate 31 upset 180 °, thereby accomplish the turn-over of the culture dish on mounting plate 31, the high temperature air through in the high temperature venthole 11b sprays on the culture dish surface, can carry out high temperature sterilization to the culture dish.
In the above step S5, when the disinfecting shelf assembly is controlled to be transported from the first runner 51 to the second runner 52, the second electric pushing rod 923 is controlled to be fully extended, so as to drive the second shifting plate 921 to swing, thereby, the shaft 34 enters the second track 173, causing the mounting plate 31 to follow the second wheel 52, when the shaft 34 moves to a position where the right rotary groove 423 and the second rotary groove 521 are aligned, the third electric push rod 933 is controlled to be contracted, when the right radial plate 421 is not obstructing the rotation of the third dial 931, the third electric push rod 933 is fully extended, the third dial 931 is rotated to push the shaft 34 into the second track 173, which follows the second runner 52, after the shaft 34 enters the second track 173, the third electric push rod 933 drives the third shifting plate 931 to reset, avoiding touching the right radial plate 421, since the second electric push rod 923 is kept fully extended at this time, the second plate 921 pushes the shaft 34 to rotate along the second track 173.
In the above S6 of this embodiment, when the shaft 34 rotates along the second track 173 and the third gear 33 is engaged with the second incomplete gear 15, the third gear 33 can be driven to rotate 180 °, and the mounting plate 31 can be driven to turn 180 °, so as to complete the turning of the culture dish on the mounting plate 31, and the ultraviolet rays emitted by the ultraviolet lamp 71 irradiate the surface of the culture dish.
In the above-mentioned S7 of the present embodiment, when the sterilizing shelf assembly is controlled to move from the second rotating wheel 52 to the right rotating wheel 42, the third electric push rod 933 is controlled to be kept fully contracted when the shaft 34 moves to the position where the right rotating groove 423 and the second rotating groove 521 are aligned, so that the third dial 931 pushes the shaft 34 into the right rail 174 to rotate along with the right rotating wheel 42.
In addition, when a culture dish is disinfected in the equipment, if a new culture dish needs to be added into the equipment for disinfection, the first motor 8 drives the left rotating wheel 41 to rotate, so that the unused mounting plate 31 rotating along with the left rotating wheel 41 rotates to a position right opposite to the left arc-shaped door plate 21, then the left arc-shaped door plate 21 is opened, due to the action of the left radial plate 411, a disinfection area in the disinfection box 1 is not communicated with the outside, then the mounting plate 31 is loaded, after the loading is completed, the first motor 8 is started to drive the left rotating wheel 41 to drive the mounting plate 31 to rotate, and the newly mounted mounting plate 31 enters the first rail 172 to be disinfected together with the original mounting plate 31 under the selection of the first shifting plate 911; through the selective action of second group's board 921, can select the mounting panel 31 to advance like in second track 173 or stay in first track 172, when original mounting panel 31 disinfected the end, through the selective action of third group's board 931, the mounting panel 31 that control disinfection ended gets into right track 174, when mounting panel 31 in right track 174 moved to and aligns with right arc door plant 22, first motor 8 stopped, can open right arc door plant 22, owing to keep apart the effect of right radial plate 421, make disinfection district and external world separate, then the manual work can take off the culture dish that disinfection ended and accomplish midway unloading.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A stem cell culture dish disinfection system is characterized by comprising a disinfection frame assembly and a disinfection box with an opening at the upper end, wherein the disinfection box comprises a main box body in the middle, a left semicircular box body arranged at the left end of the main box body and a right semicircular box body arranged at the right end of the main box body, a left arc-shaped door plate is arranged at the left side of the left semicircular box body, and a right arc-shaped door plate is arranged at the right side of the right semicircular box body; the bottom of the disinfection box is sequentially provided with a left rotating hole, a first rotating hole, a second rotating hole and a right rotating hole from left to right; the upper end of the disinfection box is fixedly provided with a top cover, and the lower end of the disinfection box is fixedly provided with a bottom plate which is plugged on the left rotating hole, the first rotating hole, the second rotating hole and the right rotating hole; a left rotating wheel positioned in the left rotating hole is rotatably connected at the circle center of the left semicircular box body in the disinfection box, and a right rotating wheel positioned in the right rotating hole is rotatably connected at the circle center of the right semicircular box body in the disinfection box; a high-temperature disinfection component is arranged on the right side of the left rotating wheel in the top cover, and an ultraviolet disinfection component is arranged on the left side of the right rotating wheel; a first rotating wheel positioned in the first rotating hole is rotatably connected to the bottom plate under the high-temperature disinfection component, and a second rotating wheel positioned in the second rotating hole is arranged under the ultraviolet disinfection component; the disinfection frame assembly is located the disinfect box and is used for placing the culture dish, and under the action of left runner, first runner, second runner, right runner, the disinfection frame assembly drives the culture dish and passes through high temperature disinfection subassembly and ultraviolet ray disinfection subassembly in proper order.
2. The stem cell culture dish sterilization system of claim 1, wherein a left rotating shaft is rotatably connected between the top cover and the bottom plate and is positioned at the center of the left rotating hole, the left rotating wheel is fixedly installed on the left rotating shaft, a plurality of left radial plates are uniformly arranged on the outer side of the left rotating wheel at intervals along the circumferential direction, and when the left radial plates rotate around the left rotating shaft, the left radial plates abut against the inner side wall of the left semicircular box body; rotate between top cap and the bottom plate and be connected with the right pivot axle that the hole centre of turning to the right side department, right side runner fixed mounting is in the pivot of turning to the right side, the outside of right side runner is equipped with a plurality of right radial plates along the circumference interval uniformly, when right radial plate rotated around the pivot of turning to the right side, right radial plate contradicted on the inside wall of right semicircle box.
3. The stem cell culture dish disinfection system of claim 2, wherein the circumferential outer side of the first rotating wheel is evenly provided with a plurality of left avoidance slots at circumferential intervals for the left radial plates to extend into, and the circumferential outer side of the second rotating wheel is evenly provided with a plurality of right avoidance slots at circumferential intervals for the right radial plates to extend into.
4. The stem cell culture dish sterilization system of claim 2, wherein the bottom plate is rotatably connected with a first rotating shaft at a center of a first rotating hole and a second rotating shaft at a center of a second rotating hole, the first rotating wheel is fixedly installed on the first rotating shaft, and the second rotating wheel is fixedly installed on the second rotating shaft.
5. The stem cell culture dish disinfection system of claim 4, wherein the lower end of the left rotating shaft extends out of the bottom plate and is fixedly provided with a left gear, the lower end of the first rotating shaft extends out of the bottom plate and is fixedly provided with a first gear meshed with the left gear, the lower end of the second rotating shaft extends out of the bottom plate and is fixedly provided with a second gear meshed with the first gear, and the lower end of the right rotating shaft extends out of the bottom plate and is fixedly provided with a right gear meshed with the second gear; the lower extreme of bottom plate is equipped with the support frame, it is used for driving left pivot pivoted first motor to fix on the support frame.
6. The stem cell culture dish sterilization system of claim 1, wherein a left track is formed between the left wheel and the left wheel, a first track is formed between the first wheel and the first wheel, a second track is formed between the second wheel and the second wheel, and a right track is formed between the right wheel and the right wheel; the disinfection frame assembly comprises a shaft lever, a left rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the left rotating wheel, a first rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the first rotating wheel, a second rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the second rotating wheel, and a right rotating groove for the shaft lever to extend into is formed in the outer side of the circumference of the right rotating wheel; the upper end of the shaft lever is provided with a limiting convex edge which is positioned above the first rotating wheel and the second rotating wheel and is used for being clamped into the outer sides of the left rotating wheel and the right rotating wheel, the upper end of the limiting convex edge is provided with an upward extending mounting plate, and a plurality of mounting holes for placing culture dishes are formed in the mounting plate; a third gear is fixedly arranged at the upper end of the mounting plate;
a first rotating rod is arranged at the joint of the left rotating hole and the first rotating hole in the bottom plate, a first shifting plate is fixedly arranged at the upper end of the first rotating rod, a first connecting rod is fixedly arranged at the lower end of the first rotating rod, a first electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the first electric push rod is hinged to the first connecting rod; when the first electric push rod is completely contracted, the left rotating wheel drives the shaft lever to move in the left track, and when the first electric push rod is completely extended out, the first shifting plate guides the shaft lever to enter the first track from the left track to move;
a second rotating rod is arranged at the joint of the first rotating hole and the second rotating hole in the bottom plate, a second shifting plate is fixedly arranged at the upper end of the second rotating rod, a second connecting rod is fixedly arranged at the lower end of the second rotating rod, a second electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the second electric push rod is hinged to the second connecting rod; when the second electric push rod is completely contracted, the first rotating wheel drives the shaft lever to move in the first track, and when the second electric push rod is completely extended out, the second shifting plate guides the shaft lever to enter the second track from the first track to move;
a third rotating rod is arranged at the joint of the second rotating hole and the right rotating hole in the bottom plate, a third shifting plate is fixedly arranged at the upper end of the third rotating rod, a third connecting rod is fixedly arranged at the lower end of the third rotating rod, a third electric push rod is hinged to the lower end of the bottom plate, and the telescopic end of the third electric push rod is hinged to the third connecting rod; when third electric putter stretches out completely, the second runner drives the axostylus axostyle and moves in the second track, and when first electric putter contracts completely, the third is dialled the board and is guided the axostylus axostyle and gets into right track internal motion by the second track.
7. The stem cell culture dish disinfection system of claim 6, wherein the side wall of the mounting hole is provided with a mounting groove, a pressing block is hinged in the mounting groove, and a spring piece is used for forcing the pressing block to extend into the mounting hole; the pressing block is used for pressing the culture dish in the mounting hole.
8. The stem cell culture dish sterilization system of claim 6, wherein the high temperature sterilization assembly comprises a convex column which is arranged on the top cover downwards along the vertical direction, a high temperature air inlet hole which is arranged vertically is arranged in the convex column, and a high temperature air outlet hole which is communicated with the high temperature air inlet hole is arranged on the outer side of the circumference of the convex column; the outside symmetry of projection is equipped with first incomplete gear, first incomplete gear is used for driving the axostylus axostyle rotation with the third gear engagement.
9. The stem cell culture dish sterilization system of claim 6, wherein the ultraviolet sterilization assembly comprises a convex plate which is arranged on the top cover downwards along the vertical direction, and a plurality of ultraviolet lamps are fixedly arranged on the outer side of the convex plate; the fixed second incomplete gear that is equipped with in the outside of flange, second incomplete gear is used for driving the axostylus axostyle rotation with the third gear engagement.
10. A method of sterilizing a culture dish using the stem cell culture dish sterilization system of claim 1, comprising the steps of:
s1: introducing high-temperature air from the high-temperature air inlet hole, spraying the high-temperature air into the disinfection box through the high-temperature air outlet hole, and then opening all the ultraviolet lamps;
s2: placing the culture dishes to be disinfected after the left arched door panel is opened in the disinfection frame assembly, closing the left arched door panel, controlling the left rotating wheel to drive the next disinfection frame assembly to be opposite to the left arched door panel, stopping the rotation of the left rotating wheel, opening the left arched door panel, manually installing the culture dishes in the next disinfection frame assembly, and sequentially operating until all the culture dishes are completely loaded;
s3: controlling the left rotating wheel to drive the disinfecting frame assembly to be conveyed to the outer side of the first rotating wheel;
s4: the first rotating wheel drives the disinfection frame assembly to rotate, and the high-temperature disinfection assembly sprays high-temperature air on the surface of the culture dish to disinfect the culture dish at high temperature;
s5: after the culture dish is sterilized at high temperature, the first rotating wheel is controlled to drive the sterilizing frame assembly to be conveyed to the outer side of the second rotating wheel;
s6: the second rotating wheel drives the disinfection frame assembly to rotate, and ultraviolet rays emitted by the ultraviolet disinfection assembly irradiate the surface of the culture dish to disinfect the culture dish by the ultraviolet rays;
s7: after the culture dish finishes ultraviolet disinfection, controlling the second rotating wheel to drive the disinfection frame assembly to be transported to the outer side of the right rotating wheel;
s8: when disinfecting frame subassembly rotated to and aligns with right arc door plant, opened right arc door plant and take off the culture dish after the disinfection, accomplished the unloading.
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Effective date of registration: 20240401 Address after: 450000, 5th floor, Building B, Jinquan Building, No. 49 Jinrong Road, Zhongmou County, Zhengzhou City, Henan Province Applicant after: Henan Zhongxu Regenerative Medicine Research Institute Co.,Ltd. Country or region after: China Address before: Xingzhi Science and Technology Park, No. 6 Xingzhi Road, Qixia District, Nanjing City, Jiangsu Province 210000 Applicant before: Nanjing Shantaiyin Biotechnology Co.,Ltd. Country or region before: China |