CN114947696A - White light-narrow band-fluorescence integrated endoscope and use method thereof - Google Patents

Abstract

The invention provides a white light-narrow band-fluorescence integrated endoscope and a using method thereof, wherein the white light-narrow band-fluorescence integrated endoscope comprises the following steps: a light source part, a light source control unit and an endoscope part; the light source part provides illumination light irradiated on the biological tissue to be detected, the illumination light comprises white light and multiple wavelengths of narrow-band light, and the narrow-band light comprises fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the detected biological tissue to generate fluorescence; the light source control unit controls the type of illumination light provided by the light source section; the endoscope part comprises an endoscope detector and a trap film; the endoscope detector is used for receiving reflected light of the illuminating light reflected by the detected biological tissue and fluorescent light generated by exciting a photosensitizer on the detected biological tissue by the fluorescence exciting light, and the reflected light and the fluorescent light enter the endoscope detector through the notch film; the trap wavelength of the trap plate is equal to the wavelength of the fluorescence excitation light. The multispectral narrowband function and the fluorescence function are combined, so that the complexity of the endoscope structure can be obviously reduced on the premise of ensuring the imaging quality and the acquisition speed.

Description

White light-narrow band-fluorescence integrated endoscope and using method thereof

Technical Field

The invention belongs to the field of endoscopes, and particularly relates to a white light-narrow band-fluorescence integrated endoscope and a using method thereof.

Background

The white light endoscope, the narrow band endoscope, the polarization endoscope and the fluorescence endoscope can respectively provide structural signals and functional signals of tumors, particularly, the narrow band signals are sensitive to functional information such as blood oxygen and blood capillaries under mucous membranes generally, the polarization signals are sensitive to chiral molecules and birefringence signals, the fluorescence signals can further prompt the tumors through the functional information such as photosensitizer probe concentration on the basis, various functional signals complement each other, and fusion analysis is performed on multi-source data through image recognition and signal filtering technologies, so that the recognition capability of an algorithm to the tumors is improved.

However, at present, the probes or imaging modules of the dye endoscope, the confocal micro-amplification endoscope, the ultrasonic endoscope and the like are not compatible with the white light endoscope, and the difficulty of multi-mode detection is increased in fact. For example, the qualitative diagnosis of digestive tract tumor is often difficult only by using a white light endoscope, and in particular, in many cases, the qualitative diagnosis of tumor needs to be further performed by using a magnifying endoscope, a staining endoscope and a pathological biopsy, and sometimes, the auxiliary judgment needs to be performed on the tissue under the mucosa. As another example, in the course of photodynamic therapy, oxygen level and photosensitizer concentration detection are two important components of photodynamic dose monitoring. The detection of oxygen content requires the use of a multispectral narrow-band endoscope for hemoglobin detection, while the concentration of photosensitizer requires a fluorescence channel for detection. In the prior art, the target tissue is difficult to be integrally detected under the same endoscope, and the switching needs to be carried out through a mechanical device. The working property of the endoscope determines that the volume of the endoscope module is inevitably increased through a mechanical device, so that the application range of the endoscope module is limited in fact. For example, a white light-microscope integrated endoscope needs to be designed with a complex endoscope-zooming light path, and the volume of the endoscope is at least doubled. The narrow-band fluorescence endoscope needs to be provided with a light splitting light path, so that the size is greatly increased, and the utilization rate of illumination of other wave bands is reduced. Therefore, no integrated endoscope is available in commercial application in the market at present. For example, the NBI endoscope of olympus typically detects narrow band reflected light at 415 and 540nm, and the open photoelectric composite dye imaging technique (VIST) performs composite dye using a single wavelength in combination with the RGB channel of the imaging CCD. These methods focus on the absorption caused by microvessels and fail to detect spectroscopic information such as lipids or autofluorescence. The british institute of science and technology developed a hyperspectral narrowband endoscope using a supercontinuum light source, but the supercontinuum light source is very expensive.

Concentration monitoring of photosensitizers is the most important part of photodynamic dose monitoring. By detecting the fluorescence intensity of the photosensitizer, various information can be obtained, such as the concentration, targeting property, spatial distribution and the like of the photosensitizer. The main use for the detection of photosensitizer concentration is fluorescence endoscopy. Patent No. CN 110772208A discloses a fluorescence endoscope apparatus, which collects multiple fluorescence image sub-blocks of a subject by a compound eye imaging apparatus, performs pixel coordinate registration on these fluorescence sub-blocks, and performs pixel restoration to superposition based on the pixel registration coordinates of each pixel point in each fluorescence image sub-block, thereby generating a fluorescence image of the subject. Although the method can acquire a high-quality fluorescence image, a plurality of detection channels are needed, and the volume is difficult to reduce, so that the scene is extremely limited in practical application, and meanwhile, the algorithm is extremely complex, and the performance requirement on the image processing equipment is high.

Patents CN109758094A and CN108577791A disclose a fluorescence navigation endoscope system and a method for enhancing fluorescence imaging sensitivity thereof, in which fluorescence and white light are separated by a light splitting optical path, and it is difficult to combine with a narrow-band reflection endoscope, because each time an optical channel is added, on one hand, more cameras and light splitting optical paths are required, which greatly increases the cost, and on the other hand, it is difficult for an endoscope probe to integrate more optical devices.

Patent document CN109758094A discloses a microscopic endoscopic system, which uses an imaging fiber to extract an image, thereby realizing optical path splitting in vitro, so that although the problem of internal volume limitation is not encountered, the resolution of the imaging fiber is limited, for example, the leading imaging fiber in the living optical industry can only realize resolution at the hundred thousand pixel level, which is far different from the current clinical 1080P or even 4K resolution requirements. Patent document CN108670203A discloses a narrow-band fluorescence imaging optical path, which uses 3 sensors and corresponding beam splitting optical paths, and this greatly increases the volume and complexity of the design, and is difficult to apply to electronic endoscopes. Patent document CN110731748A discloses an electronic endoscope with a coupled narrow-band light and a fluorescence, which uses two light splitting paths and an imaging chip, and the upper limit of the electronic endoscope can process a certain single-wavelength reflected light and a single-wavelength fluorescence.

Disclosure of Invention

The invention aims to provide a white light-narrow band-fluorescence integrated endoscope and a using method thereof, which combine a white light function, a multispectral narrow band function and a fluorescence function together, can obviously reduce the complexity of the structure of the endoscope on the premise of ensuring the imaging quality and the acquisition speed,

the invention is realized by the following technical scheme:

a white light-narrow band-fluorescence integrated endoscope, comprising: a light source part, a light source control unit and an endoscope part;

the light source part is used for providing illumination light irradiated on the biological tissue to be detected, the type of the illumination light comprises white light and narrow-band light with multiple wavelengths, and the narrow-band light comprises fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be detected to generate fluorescence;

the light source control unit is used for controlling the type of the illumination light provided by the light source part;

the endoscope part comprises an endoscope detector and a trap film; the endoscope detector is used for receiving reflected light of illumination light provided by the light source part after the illumination light is reflected by the detected biological tissue and fluorescent light generated by the excitation of the photosensitizer on the detected biological tissue by the fluorescence excitation light provided by the light source part to obtain light signals, and the reflected light and the fluorescent light enter the endoscope detector through the notch film; the trap wavelength of the trap plate is equal to the wavelength of the fluorescence excitation light.

Preferably, the scope part still includes the holder, and the inside scope detector passageway of having seted up of holder installs the scope detector in the scope detector passageway, is provided with the recess on the one end terminal surface of holder, and the recess is coaxial and the intercommunication with scope detector passageway, installs the wave plate that caves in the recess.

Furthermore, a light guide beam channel is arranged on the holder, and the axis of the light guide beam channel is parallel to the axis of the endoscope detector channel; the illumination light provided by the light source part is irradiated on the biological tissue to be detected through the light guide beam channel.

Furthermore, two light guide beam channels are arranged and are symmetrical about the endoscope detector channel.

Preferably, the endoscope tissue image acquisition system further comprises a data acquisition part, wherein the data acquisition part comprises an acquisition card and a memory card, and the acquisition card is used for receiving an optical signal of the endoscope detector and an imaging mode signal of the light source control unit and processing the optical signal and the imaging mode signal to obtain a tissue image; the memory card is used for storing the tissue image obtained by the acquisition card.

Furthermore, the device also comprises a display, and the display is used for displaying the tissue image obtained by the acquisition part in real time.

Preferably, the light source part comprises a xenon lamp light source, a focusing lens group and a filter wheel; the focusing lens group comprises a first focusing lens and a second focusing lens; a first focusing lens, a filter wheel and a second focusing lens are sequentially arranged in the emitting light direction of a xenon lamp light source.

Preferably, the light source part comprises a xenon lamp light source, a focusing lens group and a tunable liquid crystal filter LCTF; the focusing lens group comprises a first focusing lens and a second focusing lens; the first focusing lens, the tunable liquid crystal filter LCTF and the second focusing lens are sequentially arranged in the emitting light direction of the xenon lamp light source.

The using method of the white light-narrow band-fluorescence integrated endoscope comprises a white light imaging mode, a narrow band imaging mode and a fluorescence imaging mode;

if the white light imaging mode is selected, the light source control unit controls the light source part to emit white light which is irradiated on the biological tissue to be detected through the light guide beam channel, and the reflected light of the white light reflected by the biological tissue to be detected is detected through the endoscope detector;

if the narrow-band imaging mode is selected, the light source control unit controls the light source part to emit narrow-band light with the wavelength different from the trap wavelength of the trap film, the narrow-band light irradiates on the detected biological tissue through the light guide beam channel, and reflected light of the narrow-band light reflected by the detected biological tissue is detected through the endoscope detector;

if the fluorescence imaging mode is selected, the light source control unit controls the light source part to emit narrow-band light with the wavelength equal to the notch wavelength of the notch plate, the narrow-band light is irradiated on the biological tissue to be detected through the light guide beam channel, the photosensitizer on the biological tissue to be detected is excited to generate fluorescence, the narrow-band light is irradiated on the inner mirror part together with the fluorescence through reflected light reflected by the biological tissue to be detected, and the fluorescence is detected through the inner mirror detector after the reflected light is filtered by the notch plate.

Further, the white light-narrow band-fluorescence integrated endoscope comprises a data acquisition part, wherein the data acquisition part comprises an acquisition card and a memory card, and the acquisition card is used for receiving optical signals of an endoscope detector and imaging mode signals of a light source control unit and processing the optical signals and the imaging mode signals to obtain a tissue image; the storage card is used for storing the tissue image obtained by the acquisition card;

the acquisition card obtains an image tag according to an imaging mode signal fed back by the light source control unit, obtains an image according to received optical signal processing of the endoscope detector, and adds the image tag to the image to obtain a tissue image.

Compared with the prior art, the invention has the following beneficial effects:

according to the endoscope, the light source part can provide white light and narrow-band light with various wavelengths including fluorescence excitation light, namely, a complex narrow-band light splitting light path is placed at a light source end and does not enter the body, and the complex narrow-band light splitting light path can be expanded at the later stage according to requirements; the endoscope part is provided with the trap wave plate, when the illumination light is fluorescence excitation light, the reflected light of the fluorescence excitation light can be filtered, only the fluorescence is allowed to enter the endoscope detector, and the reflected light of the fluorescence excitation light is separated from the fluorescence, so that the endoscope part entering the body is simplified through a trap light path, and the design of the white light-narrow band-fluorescence integrated endoscope is realized through a small-volume light path on the basis of not increasing the volume of the endoscope part, so that the endoscope part extending into the body has small volume and simple structure; the reflected light of the fluorescence excitation light in the fluorescence mode is filtered in a trap mode, and the problem that the multispectral narrowband function and the fluorescence imaging function are difficult to integrate is solved. The invention realizes the integration of the white light endoscope, the multispectral narrow-band endoscope and the fluorescence endoscope, the white light endoscope, the multispectral narrow-band endoscope and the fluorescence endoscope share one set of light source and one set of image acquisition equipment, realizes the integration of multiple functions and fills the blank in the field. The invention has reasonable scheme, simple structure, easy realization, strong adaptability and wide application scene, can be used for detecting the oxygen content and the concentration of the photosensitizer in the photodynamic therapy process and improves the integration degree of the endoscope for the photodynamic therapy of deep tumors.

Furthermore, the endoscope part of the invention adopts a clamp holder which is designed independently, and has ingenious structure and low manufacturing cost.

Furthermore, the data acquisition part adopts an integrated acquisition card and a memory card, so that the data acquisition part can acquire and store data and has small volume.

The white light-narrow band-fluorescence integrated endoscope can realize simple switching of a white light imaging mode, a narrow band imaging mode and a fluorescence imaging mode during use, and can realize multiple detection modes by adopting the same endoscope.

Drawings

FIG. 1 is a block diagram of the overall structure of a white light-narrow band-fluorescence endoscope imaging system according to the present invention;

FIG. 2 is a schematic view of a portion of an endoscope in accordance with the present invention;

FIG. 3 is a schematic view of a holder according to the present invention;

FIG. 4 is a schematic view of a light source according to a first embodiment of the present invention;

FIG. 5 is a schematic view of a light source according to a second embodiment of the present invention;

FIG. 6 is a flow chart of time-sequential gating for three modes of the present invention;

the light source part 100, the endoscope part 200, the data acquisition part 300, the endoscope detector 210, the notch plate 230, the clamper 240, the light guide beam channel 241/242, the endoscope detector channel 243, the groove 244, the xenon lamp light source 110, the focusing lens group 120, the filter wheel 130 and the tunable liquid crystal filter LCTF 140.

Detailed Description

For a further understanding of the invention, reference will now be made to the following examples, which are provided to illustrate further features and advantages of the invention, and are not intended to limit the scope of the invention as set forth in the following claims.

The invention relates to a white light-narrow band-fluorescence endoscope imaging system, which realizes the integrated design of narrow band reflection imaging and fluorescence imaging. The concept is to simplify the optical design of the endoscope part, separate fluorescence from narrow-band light reflection wave bands by trapping, realize white light-narrow-band light source excitation in an external light source without increasing the volume of the endoscope part needing to enter the body, and further realize the target of white light-narrow-band-fluorescence integrated endoscope detection.

As shown in fig. 1, the white light-narrow band-fluorescence endoscope imaging system of the present invention is composed of three parts: a light source part 100, a light source control unit, an endoscope part 200, and a data acquisition part 300.

A light source section 100 for providing illumination light to be irradiated on a subject biological tissue, the illumination light being of a type including white light and narrow-band light of a plurality of wavelengths, the narrow-band light including fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be detected to generate fluorescence. The wavelength of the fluorescence excitation light needs to be selected according to the characteristics of the photosensitizer.

The light source control unit is used to control the type of illumination light provided by the light source section 100.

The endoscope part 200, which is a probe part extending into the biological tissue to be detected, can capture and collect the tissue image of the biological tissue to be detected under the white light or narrow-band light illumination provided by the light source part 100 to obtain an optical signal.

And a data acquisition part 300 which receives the light signal of the endoscope part 200 and the imaging mode signal of the light source control unit, processes the image of the tissue and stores the image.

As shown in FIG. 2, the endoscopic portion 200 is comprised of an endoscopic probe 210, a notch plate 230, the notch plate 230 having a notch wavelength equal to the wavelength of the fluorescence excitation light. The endoscope detector 210 and the notch plate 230 are fixed together by a holder 240, and the holder 240 is provided with a light beam channel which can extend into human tissues for detection. The holder 240 is constructed as shown in fig. 3, and is designed using SOLIDWORKS software, and 3D printed PLA material. The part adopts split type printing, and is divided into a front part and a rear part, and has three channels, wherein two channels 241 and 242 with smaller diameters at two sides are light guide beam channels, and a channel 243 with a larger diameter at the middle is an endoscope detector channel and is used for installing the endoscope detector 210. At the front end of the endoscopic probe channel (near the biological tissue being examined) is a notch 244 which serves as a stationary notch plate 230.

The endoscopic probe 210 is used for receiving the reflected light of the illumination light provided by the light source portion 100 after being reflected by the biological tissue to be detected and the fluorescence generated by the photosensitizer on the biological tissue to be detected being excited by the fluorescence excitation light provided by the light source portion 100. When the light source portion 100 provides fluorescence excitation light, since the notch wavelength of the notch plate 230 is equal to the wavelength of the fluorescence excitation light, the notch plate can filter the reflected light of the detected biological tissue, and it can be ensured that only fluorescence reaches the endoscope detector during fluorescence imaging.

The design of the holder 240 can ensure that the reflected light and the fluorescence are separated, so that the purpose of filtering the reflected light of the tissue when the fluorescence function imaging is realized is ensured, and the holder is a key link for ensuring the integration of the fluorescence imaging function and the narrow-band imaging function.

The resolution of the endoscopic probe 210 is optionally 1920 x 1080.

The invention can be used with various light sources, as long as the light source can emit narrow-band light with different wavelengths. The endoscope can be matched with a hyperspectral and hyperspectral light source, widens the application range of hyperspectral and hyperspectral technologies, combines multispectral, hyperspectral and even hyperspectral technologies with an endoscope, and overcomes the defect that the endoscope is difficult to apply in vivo due to limited light penetrability.

As shown in fig. 4, in one possible light source design, the light source portion 100 uses a design of the xenon light source 110+ the focusing lens group 120+ the filter wheel 130. The focusing lens, the filter wheel 130, and the focusing lens are disposed in this order in the light emitting direction of the xenon light source 110. The focusing lens can focus light with the diameter of about 60mm emitted by the xenon lamp into the light beam with the diameter of 4mm, and the light beam is focused on the light incoming surface of the light guide beam channel. Meanwhile, the filter wheel is provided with a through hole for transmitting white light and a plurality of filters with different wavelengths, and the filters can select and pass required narrow-band waves from light of a wide spectrum band emitted by the xenon lamp, so that the purpose of narrow-band imaging is achieved, and a proper filter wheel is selected according to requirements when the xenon lamp is used. As shown in fig. 5, in another possible light source design, a xenon light source 110+ a focusing lens group 120+ a tunable liquid crystal filter LCTF140 design is used. Compared with a filter wheel, the LCTF has the advantages of more accessible wavelengths and higher controllability. These two possible light source designs can be exemplified as multispectral and hyperspectral light sources, respectively. Of course, the present invention does not limit the specific parameters and optical path structure of the light source, and can be used as a light source as long as it can emit white light and narrow-band light with different wavelengths, such as a super-continuum spectrum light source. In addition to the two possibilities listed above, it is also possible to use an LED, spectrometer or the like as a light source component.

The data acquisition part 300 comprises an acquisition card and a memory card, wherein the acquisition card is used for receiving the optical signal of the endoscopic detector 210 and the imaging mode signal of the light source control unit and processing the optical signal and the imaging mode signal to obtain a tissue image; the storage card is used for storing the tissue image obtained by the acquisition card; the acquisition card obtains an image tag according to an imaging mode signal fed back by the light source control unit, obtains an image according to received optical signal processing of the endoscope detector 210, adds the image tag to the image to obtain a tissue image, and can be used for processing a subsequent machine learning algorithm to reconstruct different functional images. For example, an image acquired in the narrow band mode may be used for discrimination of blood oxygen saturation, and an image acquired in the fluorescence mode may be used for discrimination of a photosensitizer dose before and after photodynamic therapy.

The data acquisition part adopts an integrated acquisition card and a memory card, has small volume, is provided with an operating handle and a UDB peripheral connecting groove, can be connected with a peripheral USB for storing images, and is convenient to operate. The acquisition card has a real-time imaging function, can be externally connected with a display to display real-time endoscopic images, and can freeze the images through the operation handle of the acquisition card, and the frozen images can be stored in an externally connected USB device through the storage card.

The light path of the invention is subjected to simulation and actual verification of Zemax software, the part of the light path extending into the body is simple, small in size and wide in application scene, and can be used in the digestive tract and the colorectal tract of small and medium-sized animals.

The working process of the system of the invention is as follows: if the imaging mode is the white light imaging mode, the white light emitted by the light source part 100 irradiates the detected biological tissue through the light guide beam channel, the reflected light of the detected biological tissue is detected by the endoscope detector to obtain a white light image, the white light image is transmitted to the data acquisition part and is displayed by the display, and the white light image can provide visual feeling of the operation part for a doctor; if the narrow-band imaging mode is adopted, narrow-band light with the wavelength which is not equal to the notch wavelength of the notch film and is emitted by the light source part 100 is irradiated on the biological tissue to be detected through the light guide beam channel, reflected light of the biological tissue to be detected is collected through an endoscope detector in the endoscope part 200, the endoscope detector is connected with the data acquisition part 300, the real-time display and storage of narrow-band images can be carried out, the narrow-band images stored in the USB peripheral can generate a multi-spectral or hyper-spectral data set, and the judgment of the blood oxygen concentration can be carried out by combining with a corresponding algorithm; if the imaging mode is fluorescence imaging, narrow-band light (also photosensitizer exciting light) with the wavelength equal to the notch wavelength of the notch film, which is emitted by the light source part 100, is irradiated on the detected biological tissue through the light guide beam channel, reflected light and fluorescence of the detected biological tissue are irradiated on the endoscope part 200 together, the reflected light is filtered by the corresponding notch film, the fluorescence is collected through an endoscope detector in the endoscope part 200, the endoscope detector is connected with the data acquisition part 300, real-time display and storage of images can be carried out, and the fluorescence mode can be used for detecting the concentration of the photosensitizer at the operation part. The three modes of the endoscope do not need to be manually switched, only the modes need to be distinguished according to different wavelengths of used illuminating light, the wavelength of the illuminating light of a light source part is subjected to time sequence control through a program, and after a non-fluorescence excitation waveband is gated, an obtained image is marked as a narrow-band image of a corresponding waveband, and at the moment, the narrow-band image corresponds to a narrow-band imaging mode; when the fluorescence excitation band matching the notch wavelength of the notch plate is gated, a fluorescence image is obtained as the excitation band is filtered by the notch plate, which corresponds to the fluorescence mode. In short, it is only necessary to ensure that the wavelength of the excitation narrowband light of the fluorescence mode is just the notch wavelength of the notch plate. The flow chart of the timing gating of the three modes is shown in fig. 6.

The wavelength of the narrow band light is not limited in the present invention, and specific wavelength parameters need to be selected according to the optical characteristics of the biological tissue to be examined. Because the invention is originally designed to measure the oxygen content and the concentration of the photosensitizer in the process of photodynamic therapy, the center wavelength of the narrow-band light is preferably near the absorption peak value of blood oxyhemoglobin and deoxyhemoglobin, and the wavelength of the fluorescence excitation light is preferably the excitation wavelength of the photosensitizer which is common in photodynamic therapy.

Claims (10)

1. A white light-narrow band-fluorescence integrated endoscope, comprising: a light source part (100), a light source control unit, and an endoscope part (200);

the light source part (100) is used for providing illumination light irradiated on the detected biological tissue, the type of the illumination light comprises white light and narrow-band light with multiple wavelengths, and the narrow-band light comprises fluorescence excitation light; the fluorescence excitation light can excite the photosensitizer on the biological tissue to be detected to generate fluorescence;

a light source control unit for controlling the type of illumination light provided by the light source section (100);

the endoscopic portion (200) includes an endoscopic probe (210) and a notch plate (230); the endoscope detector (210) is used for receiving reflected light of the illumination light provided by the light source part (100) after being reflected by the biological tissue to be detected and fluorescence generated by the excitation of the photosensitizer on the biological tissue to be detected by the fluorescence excitation light provided by the light source part (100) to obtain an optical signal, and the reflected light and the fluorescence enter the endoscope detector (210) through the notch film (230); the notch wavelength of the notch plate (230) is equal to the wavelength of the fluorescence excitation light.

2. The white light-narrow band-fluorescence integrated endoscope of claim 1, wherein the endoscope portion (200) further comprises a holder (240), an endoscope probe channel (243) is formed inside the holder (240), an endoscope probe (210) is installed in the endoscope probe channel (243), a groove (244) is formed in an end face of one end of the holder (240), the groove (244) is coaxial and communicated with the endoscope probe channel (243), and a wave trap (230) is installed in the groove (244).

3. The integrated endoscope of claim 2, characterized in that the holder (240) is provided with a light guide channel, and the axis of the light guide channel is parallel to the axis of the endoscope probe channel (243); the illumination light provided by the light source part (100) is irradiated on the biological tissue to be detected through the light guide beam channel.

4. The integrated white light-narrow band-fluorescence endoscope of claim 3, wherein two light guide beam channels are provided, and the two light guide beam channels are symmetrical with respect to the endoscope probe channel (243).

5. The integrated endoscope of claim 1, further comprising a data collection unit (300), wherein the data collection unit (300) comprises an acquisition card and a memory card, the acquisition card is used for receiving the optical signal of the endoscope detector (210) and the imaging mode signal of the light source control unit, and processing the optical signal and the imaging mode signal to obtain a tissue image; the memory card is used for storing the tissue images obtained by the acquisition card.

6. The integrated white light-narrow band-fluorescence endoscope of claim 5, further comprising a display for displaying the tissue image obtained by the acquisition portion (300) in real time.

7. The white light-narrow band-fluorescence integrated endoscope of claim 1, wherein the light source section (100) comprises a xenon lamp light source (110), a focusing lens group (120), and a filter wheel (130); the focusing lens group (120) comprises a first focusing lens and a second focusing lens; a first focusing lens, a filter wheel (130) and a second focusing lens are arranged in this order in the direction of light emitted from a xenon light source (110).

8. The integrated white-light-narrow-band-fluorescence endoscope of claim 1, characterized in that the light source section (100) comprises a xenon lamp light source (110), a focusing lens group (120) and a tunable liquid crystal filter LCTF; the focusing lens group (120) comprises a first focusing lens and a second focusing lens; the first focusing lens, the tunable liquid crystal filter LCTF and the second focusing lens are sequentially arranged in the emitting light direction of the xenon lamp light source (110).

9. The method for using the white light-narrow band-fluorescence integrated endoscope of any one of claims 1-8, which is characterized by comprising a white light imaging mode, a narrow band imaging mode and a fluorescence imaging mode;

if the white light imaging mode is selected, the light source control unit controls the light source part (100) to emit white light which is irradiated on the detected biological tissue through the light guide beam channel, and the reflected light of the white light reflected by the detected biological tissue is detected through the endoscope detector;

if the narrow-band imaging mode is selected, the light source control unit controls the light source part (100) to emit narrow-band light with the wavelength not equal to the trap wavelength of the trap sheet, the narrow-band light irradiates on the biological tissue to be detected through the light guide beam channel, and reflected light of the narrow-band light reflected by the biological tissue to be detected is detected through the endoscope detector;

if the fluorescence imaging mode is selected, the light source control unit controls the light source part (100) to emit narrow-band light with the wavelength equal to the notch wavelength of the notch plate, the narrow-band light is irradiated on the biological tissue to be detected through the light guide beam channel, the photosensitizer on the biological tissue to be detected is excited to generate fluorescence, the narrow-band light is irradiated on the endoscope part (200) through reflected light reflected by the biological tissue to be detected and the fluorescence together, the reflected light is filtered by the notch plate, and the fluorescence is detected through the endoscope detector.

10. The use method of the integrated endoscope comprises a data acquisition part (300), wherein the data acquisition part (300) comprises an acquisition card and a memory card, the acquisition card is used for receiving optical signals of the endoscopic probe (210) and imaging mode signals of the light source control unit, and processing the optical signals and the imaging mode signals to obtain a tissue image; the storage card is used for storing the tissue image obtained by the acquisition card;

the acquisition card obtains an image tag according to an imaging mode signal fed back by the light source control unit, obtains an image according to received optical signal processing of the endoscope detector (210), and adds the image tag to the image to obtain a tissue image.

Images