CN115721247A - Endoscopic imaging system for photodynamic diagnosis - Google Patents
Endoscopic imaging system for photodynamic diagnosis Download PDFInfo
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- CN115721247A CN115721247A CN202111015414.8A CN202111015414A CN115721247A CN 115721247 A CN115721247 A CN 115721247A CN 202111015414 A CN202111015414 A CN 202111015414A CN 115721247 A CN115721247 A CN 115721247A
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Abstract
The invention relates to the technical field of endoscopic imaging, in particular to an endoscopic imaging system for photodynamic diagnosis, which comprises an exciting light generating assembly, a fluorescence generating assembly and a processing assembly; the light path of the white light source is orthogonal to the light path of the blue light source, a dichroic mirror is arranged at the orthogonal position, and the dichroic mirror forms an angle of 45 degrees with the light path optical axis of the white light source and also forms an angle of 45 degrees with the light path optical axis of the blue light source; the dichroic mirror can transmit photons in a white light waveband and reflect photons in a blue light waveband, so that the emission light of the blue light source is reflected by the dichroic mirror and then has the same emission direction as the white light source; and the fused light beam of the white light source and the blue light source enters the endoscope module through the light guide beam after passing through the third collimating lens. The invention can simultaneously acquire the color image and the fluorescence image, doctors can carry out more comprehensive and accurate diagnosis without frequently switching the working mode, thereby saving the operation time, improving the use convenience of the equipment and reducing the omission factor.
Description
Technical Field
The invention relates to the technical field of endoscopic imaging, in particular to an endoscopic imaging system for photodynamic diagnosis.
Background
In the technical field of endoscopic imaging, optical imaging has the advantages of no damage, low cost and the like, and has a great application value, a traditional white light endoscope obtains a fine surface structure of a focal tissue through white light illumination imaging, a doctor can identify a tumor region through the abnormality of the surface structure, but the contrast between the tumor region in the early stage of lesion and a normal mucosa is low, and a missed diagnosis phenomenon is easy to occur. The fluorescence endoscope is a novel diagnosis device which uses fluorescence spectrum technology to enter cavity diagnosis, can detect tumors by detecting the enrichment condition of a tissue surface fluorescence reagent, can identify the tumor region which is not easy to be perceived under the white light endoscope due to higher sensitivity, and is an important supplementary tool of the white light endoscope.
The conventional fluorescence endoscope is a fluorescence endoscope using ICG (indocyanine green), and because the fluorescence waveband of the fluorescence endoscope is located in a near-infrared region which is not overlapped with the white light waveband, the fluorescence endoscope can be simultaneously illuminated by white light and exciting light and is subjected to light splitting and collection at a camera shooting end, so that simultaneous imaging of the white light and the fluorescence is realized.
However, the fluorescence band of the fluorescent agent used for photodynamic diagnosis, such as 5-ALA (5-aminolevulinic acid), is in the range of 620-640nm and is overlapped with white light, and the simultaneous acquisition of the white light and the fluorescence image cannot be realized by the method. In the existing means, only blue light with a wave band of 380-420nm is illuminated in a fluorescence mode and filtered by a filter at a camera shooting end, so that a finally presented picture can only see a red fluorescence area, and a surface tissue structure can hardly be seen. The doctor can confirm the diagnosis result only by frequently switching the illumination mode and respectively obtaining the fluorescence and white light images during the operation.
Therefore, it is highly desirable to design an endoscopic imaging system for photodynamic diagnosis, which realizes the spectroscopic acquisition of white light and fluorescence in the same time period, and then fuses the spectroscopic acquisition images into a washed lesion tissue image through post-processing.
Disclosure of Invention
In order to solve the above problems, the present invention provides an endoscopic imaging system for photodynamic diagnosis, which combines a white light illumination mode and a fluorescence illumination mode, so as to reduce the missing rate, improve the convenience of the device, and improve the accuracy of the examination result.
In order to realize the purpose, the technical scheme of the invention is as follows:
an endoscopic imaging system for photodynamic diagnosis, comprising an excitation light generating assembly, a fluorescence generating assembly and a processing assembly;
the excitation light generation assembly comprises a white light source, a first collimating lens and a filter which are arranged in sequence, wherein the filter is a band elimination filter;
the fluorescence generation assembly comprises a blue light source and a second collimating lens which are arranged in sequence;
the processing assembly comprises an endoscopic module, a switching lens, a camera module and an image processing module which are connected in sequence;
the light path of the white light source is orthogonal to the light path of the blue light source, a dichroic mirror is arranged at the orthogonal position, and the dichroic mirror forms an angle of 45 degrees with the light path optical axis of the white light source and also forms an angle of 45 degrees with the light path optical axis of the blue light source; the dichroic mirror can transmit photons of the white light wave band and reflect photons of the blue light wave band, so that the emission light of the blue light source is reflected by the dichroic mirror and then has the same emission direction as that of the white light source;
and a third collimating lens is arranged on the rear side of the dichroic mirror along the emission direction of the white light source, and a fused light beam of the white light source and the blue light source enters the endoscope module through a light guide beam after passing through the third collimating lens.
Further, the white light source is one of a laser, an LED light source, and external introduction light.
Further, the blue light source is one of a laser, an LED light source, and external introduction light.
Further, the stop band of the optical filter is 620-640nm.
Further, the optical filter is controlled to rotate by a stepping motor.
Further, the processing assembly further comprises a display for displaying the imaged image.
Further, the turning wavelength of the dichroic mirror is 420nm.
Further, the camera module comprises a beam splitter prism, a black-and-white image sensor and a color image sensor; the beam splitter prism and the imaging light entering the camera module form an angle of 45 degrees, one part of the imaging light is reflected by the beam splitter prism and then irradiates on the black-and-white image sensor, and the other part of the imaging light is transmitted by the beam splitter prism and then irradiates on the color image sensor.
Furthermore, the lower surface of the beam splitter prism corresponding to the black-and-white image sensor is plated with a 620-640nm light-transmitting film which transmits light with the wavelength within the range of 620-640nm.
Furthermore, the light guide bundle comprises a plurality of image transmission optical fiber monofilaments and an optical fiber outer sleeve wrapped on the periphery of the bundled image transmission optical fiber monofilaments.
The invention has the beneficial effects that:
1. the invention blocks a small amount of spectral information of a white light source by using a stop band filter, and fuses and irradiates white light and blue light losing a small amount of spectral information on tissues by using a dichroic mirror, after imaging light reflected by the tissues is converted by a camera module, a color image sensor acquires a white light image losing a small amount of spectral information and is used for observing surface structure information, a black-and-white image sensor acquires fluorescence information, and the white light image and the fluorescence information can be subjected to image fusion by the early-stage spatial alignment of an imaging module; the simultaneous acquisition of white light and fluorescence images in photodynamic diagnosis of photosensitizer 5-ALA and the like is realized; doctors can diagnose more comprehensively and accurately according to the information of the doctors and the medical instruments without frequently switching working modes, thereby saving operation time, improving the use convenience of equipment and reducing the omission factor.
2. The optical filter is driven by the stepping motor, the stepping motor extracts the optical filter out of the light path, the image processing unit only collects the image of the color image sensor, the system can obtain the white light image without any loss of spectral information, and the application range of the device is wider.
Drawings
FIG. 1 is a schematic diagram of the connection of the present invention.
Fig. 2 is a schematic structural diagram of the camera module according to the present invention.
Description of reference numerals:
the system comprises a white light source 1, a blue light source 2, a first collimating lens 3, a second collimating lens 4, a light filter 5, a dichroic mirror 6, a third collimating lens 7, a light guide beam 8, an endoscopic module 9, an adapter lens 10, a camera module 11, an image processing module 12, a display 13, a beam splitter prism 14, a black-and-white image sensor 15 and a color image sensor 16.
Detailed Description
The technical solutions of the present invention will be described in detail with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without any inventive work belong to the protection scope of the present invention. It is to be noted that the terms "center", "upper", "lower", "vertical", "horizontal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and operate, and thus, should not be construed as limiting the present invention.
1-2, the present invention provides an endoscopic imaging system for photodynamic diagnosis, comprising an excitation light generation assembly, a fluorescence generation assembly, and a processing assembly;
the excitation light generation assembly comprises a white light source 1, a first collimating lens 3 and a filter 5 which are arranged in sequence, wherein the filter 5 is a band elimination filter; the wavelength range of the light emitted by the white light source 1 is 420-680nm.
The fluorescence generation assembly comprises a blue light source 2 and a second collimating lens 4 which are arranged in sequence; the blue light source 2 emits light in the wavelength range of 380-420nm.
The processing assembly comprises an endoscopic module 9, a switching lens 10, a camera module 11 and an image processing module 12 which are connected in sequence;
the light path of the white light source 1 is orthogonal to the light path of the blue light source 2, a dichroic mirror 6 is arranged at the orthogonal position, and the dichroic mirror 6 forms an angle of 45 degrees with the light path optical axis of the white light source 1 and also forms an angle of 45 degrees with the light path optical axis of the blue light source 2; the dichroic mirror 6 can transmit photons in the white light wave band and reflect photons in the blue light wave band, so that the emission light of the blue light source 2 is reflected by the dichroic mirror 6 and then has the same emission direction as the white light source 1;
along the emitting direction of the white light source 1, a third collimating lens 7 is arranged at the rear side of the dichroic mirror 6, and the fused light beam of the white light source 1 and the blue light source 2 enters the endoscopic module 9 through a light guide beam 8 after passing through the third collimating lens 7.
Specifically, the white light source 1 is an LED light source, and may be a laser or external light guide.
Specifically, the blue light source 2 is an LED light source, and may also be a laser or external introduction light.
Specifically, the stop band of the optical filter 5 is 620-640nm, and when photons of the white light source 1 pass through the optical filter 5, the optical filter 5 blocks photons with the wavelength within the range of 620-640nm.
Further, the optical filter 5 is controlled to rotate by a stepping motor.
Further, the processing assembly further comprises a display 13 for displaying the imaged image.
Specifically, the turning wavelength of the dichroic mirror 6 is 420nm.
Specifically, the camera module 11 includes a beam splitter prism 14, a black-and-white image sensor 15, and a color image sensor 16; the beam splitter prism 14 forms an angle of 45 degrees with the imaging light entering the camera module 11, a part of the imaging light is reflected by the beam splitter prism 14 and then irradiates on the black-and-white image sensor 15, and the other part of the imaging light is transmitted by the beam splitter prism 14 and then irradiates on the color image sensor 16.
Further, the lower surface of the beam splitter prism 14 close to the black-and-white image sensor 15 is plated with a light-transmitting film of 620-640nm.
Specifically, the light guide bundle 8 is a conventional medical optical fiber light guide bundle or a medical liquid light guide bundle, and includes a plurality of image transmission optical fiber monofilaments and an optical fiber outer sleeve wrapped around the bundled image transmission optical fiber monofilaments.
The invention has two working modes, namely a white light mode and a fluorescence mode, wherein in the white light mode, the step motor controls the optical filter 5 to rotate, the optical filter 5 is extracted out of a white light path, the blue light source 2 does not work, the image processing module 12 only collects the image of the color image sensor 16, and the system can obtain the white light image without losing any spectral information.
In the fluorescence mode, white light emitted by a white light source 1 is collimated into parallel light by a first collimating lens 3, spectral information with the wavelength of 620-640nm is filtered by an optical filter 5, and the filtered white light penetrates through a dichroic mirror 6; meanwhile, the blue light emitted by the blue light source 2 is collimated into parallel light by the second collimating lens 4, and then the blue light is reflected by the dichroic mirror 6 to be fused with the white light; the fused light beam enters the endoscopic module 9 through the third collimating lens 7 and the light guide beam, and is irradiated on the tissue through the light guide fiber of the endoscopic module 9, the wavelength of the white light reflected by the tissue is unchanged, and the wavelength of the blue light excited by the photosensitizer in the tissue is 620-640nm. The white light reflected by the tissue and the excited fluorescence are projected into the camera module 11 through the lens group and the adapter lens 10 in the endoscope module 9 for photoelectric conversion.
The white light and the excited fluorescence are projected onto the beam splitter prism 14 of the camera module 11, the spectral information which is transmitted through the beam splitter prism 14 in parallel is irradiated to the color image sensor 16, and only the spectral information within the range of 620-640nm is irradiated to the black-and-white image sensor 15 after being reflected by the beam splitter prism 14, so that the color image sensor 16 collects a white light image, and the black-and-white image sensor 15 collects a fluorescence image. The image processing module 12 collects the image information of the color image sensor 16 and the black-and-white image sensor 15 and performs fusion processing, so that a doctor can perform more comprehensive and accurate diagnosis according to the fused image information, and the omission factor is reduced.
Experiments show that in the fluorescence mode, although the acquired white light image loses the spectral information in the range of 620-640nm, the influence on the diagnosis result is negligible; the invention can simultaneously acquire the white light image and the fluorescence image, improve the diagnosis efficiency and save 60 percent of diagnosis time under the same organization environment.
Although the present invention has been described in detail with reference to examples, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the scope of the present invention.
Claims (10)
1. An endoscopic imaging system for photodynamic diagnosis, comprising an excitation light generating assembly, a fluorescence generating assembly and a processing assembly;
the excitation light generation assembly comprises a white light source, a first collimating lens and an optical filter which are sequentially arranged, the optical filter is a band elimination optical filter, the optical filter is driven by a motor to rotate, and the optical filter is rotated out of the excitation light generation assembly in a common white light mode; in the fluorescence mode, the filter is rotated back into the excitation light generation assembly.
The fluorescence generation assembly comprises a blue light source and a second collimating lens which are arranged in sequence;
the processing assembly comprises an endoscopic module, a switching lens, a camera module and an image processing module which are connected in sequence;
the light path of the white light source is orthogonal to the light path of the blue light source, a dichroic mirror is arranged at the orthogonal position, and the dichroic mirror forms an angle of 45 degrees with the light path optical axis of the white light source and also forms an angle of 45 degrees with the light path optical axis of the blue light source; the dichroic mirror can transmit photons of the white light wave band and reflect photons of the blue light wave band, so that the emission light of the blue light source is reflected by the dichroic mirror and then has the same emission direction as that of the white light source;
and a third collimating lens is arranged on the rear side of the dichroic mirror along the emission direction of the white light source, and a fused light beam of the white light source and the blue light source enters the endoscope module through a light guide beam after passing through the third collimating lens.
2. The endoscopic imaging system according to claim 1, wherein: the white light source is one of a laser, an LED light source and external guide light.
3. The endoscopic imaging system according to claim 1, wherein: the blue light source is one of a laser, an LED light source and external introduction light.
4. An endoscopic imaging system according to claim 1, wherein: the stop band of the optical filter is 620-640nm.
5. The endoscopic imaging system according to claim 1, wherein: the motor is a stepping motor.
6. The endoscopic imaging system according to claim 1, wherein: the processing assembly also includes a display for displaying the imaged image.
7. An endoscopic imaging system according to claim 1, wherein: the turning wavelength of the dichroic mirror is 420nm.
8. The endoscopic imaging system according to claim 1, wherein: the camera module comprises a beam splitter prism, a black-and-white image sensor and a color image sensor; the beam splitter prism and the imaging light entering the camera module form an angle of 45 degrees, one part of the imaging light is reflected by the beam splitter prism and then irradiates on the black-and-white image sensor, and the other part of the imaging light is transmitted by the beam splitter prism and then irradiates on the color image sensor.
9. The endoscopic imaging system according to claim 8, wherein: the lower surface of the light splitting prism corresponding to the black-and-white image sensor is plated with a 620-640nm light-transmitting film.
10. The endoscopic imaging system according to claim 1, wherein: the light guide bundle comprises a plurality of image transmission optical fiber monofilaments and an optical fiber outer sleeve pipe wrapping the periphery of the bundled image transmission optical fiber monofilaments.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116548899A (en) * | 2023-04-19 | 2023-08-08 | 之江实验室 | Optical endoscopic diagnosis and treatment system and electronic device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116548899A (en) * | 2023-04-19 | 2023-08-08 | 之江实验室 | Optical endoscopic diagnosis and treatment system and electronic device |
| CN116548899B (en) * | 2023-04-19 | 2024-02-06 | 之江实验室 | Optical endoscopic diagnosis and treatment system and electronic device |
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