CN114940907A - Heavy metal contaminated soil remediation agent and remediation method thereof - Google Patents
Heavy metal contaminated soil remediation agent and remediation method thereof Download PDFInfo
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- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 72
- 229910001385 heavy metal Inorganic materials 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000005067 remediation Methods 0.000 title claims description 36
- 229910021426 porous silicon Inorganic materials 0.000 claims abstract description 38
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- 235000009566 rice Nutrition 0.000 claims abstract description 34
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/02—Soil-conditioning materials or soil-stabilising materials containing inorganic compounds only
- C09K17/08—Aluminium compounds, e.g. aluminium hydroxide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Life Sciences & Earth Sciences (AREA)
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- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
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- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a heavy metal contaminated soil restoration agent and a restoration method thereof, wherein the restoration agent and contaminated soil are mixed in proportion, and the restoration agent consists of porous silicon and rice straw biochar; preparing a bacteria liquid culture medium consisting of yeast extract powder, ammonium sulfate and trihydroxymethyl aminotoluene, and sequentially performing high-temperature sterilization, bacteria liquid inoculation and constant-temperature shaking culture; inoculating the cultured bacterial liquid into an optimized culture medium and culturing at constant temperature; mixing the repairing agent and the polluted soil, drying to obtain a soil sample, mixing the soil sample with the bacterial liquid, preparing a cylindrical sample, fully soaking the cylindrical sample in the cementing nutrient solution, and aerating to generate mineralization reaction; and taking out the sample after the mineralization reaction time is reached, and naturally drying the sample. The invention has the advantages that: the repairing agent is prepared by using MICP technology, porous silicon and rice straw biochar as raw materials, so that the zinc-lead polluted soil is repaired, the leaching concentration of heavy metals in the polluted soil is reduced, and the solidification/stabilization of the polluted soil is realized.
Description
Technical Field
The invention belongs to the technical field of soil remediation, and particularly relates to a heavy metal contaminated soil remediation agent and a remediation method thereof.
Background
Heavy metals are seriously harmful to plants, animals and even human beings because of the characteristics of variable forms in soil, enrichment in biological environment, strong concealment of toxicity, difficult removal from the environment and the like. Therefore, heavy metal pollution in soil has become one of the most serious environmental problems worldwide, and its repair is imminent.
The method is a new soil remediation technology combining microorganism in-situ remediation and chemical stationary phase, and is used for fixing and removing heavy metals in polluted soil by applying a MICP (microbial induced calcium carbonate precipitation) technology. For the research on solidifying/stabilizing heavy metal polluted soil by the MICP technology, the research is carried out by fixing heavy metal ions by the MICP technology, mineralizing and consolidating exchangeable heavy metal ions and sealing the exchangeable heavy metal ions in a carbonate combined state. In the literature "CHENG Yan, ZHAO Xingq. Study on the correlation and ionization of Pb 2+ by carbonate-mineralization bacteria "(Research of Environmental Sciences, 2016, 29(10): 1513- 3 And removing heavy metals in the soil. In the literature, "Wang Ruixing, Qian Chun Xiang, Wu \2815656And in the literature 'research on mineralization of heavy metal ions Zn to (2+) by Wangming, Qianqingxiang and phosphate mineralized bacteria' (functional materials, 2013,44(03):393 and 395), Qianqingxiang and the like select fixed strains to mineralize heavy metal ions in polluted soil of Zn, and research on the mineralization and consolidation of microorganisms on Zn under different pollution conditions 2+ The action and mechanism of (A). The research progress of repairing heavy metal contaminated soil by biochar (engineering geology report, 2018, 26(04): 1064. 1077.), the research on electroosmosis strengthening and electric repairing theory and test research of polluted foundation soil (Hangzhou: Zhejiang university, 2020.) and the research on space distribution and pollution evaluation of heavy metal in Yingdu mineral area soil based on GIS (Beijing: China geological university, 2018.) show that the research on repairing heavy metal contaminated soil by using various adsorption materials or biological methods has more results, but the research on repairing heavy metal contaminated soil by physical repair methods alone, Lijingwang, Kingkung, Chengdong and electric repairing theory and test research on polluted foundation soil (Hangzhou: Zhejiang university, 2020.) and the research on the stability and environmental effect of microorganism-induced carbonate deposition and chromium-contaminated soil (Yangzhou: Yangzhou university, 2019.) show that the research on repairing heavy metal contaminated soil by using various adsorption materials or biological methods has more results in the research on single physical repair method, Both chemical remediation and biological remediation have certain limitations and poor effects, so that the combination of the MICP technology and the adsorption material for remediation of contaminated soil to reduce the leaching concentration of heavy metals and improve the mechanical properties of the solidified material becomes the key point of research.
Disclosure of Invention
The invention aims to provide a heavy metal contaminated soil repairing agent and a repairing method thereof according to the defects of the prior art, the repairing method repairs the zinc-lead contaminated soil by using a repairing agent which is jointly developed by using a MICP technology and porous silicon and rice straw biochar as raw materials, and effectively solves the problem of solidification/stabilization of the contaminated soil.
The purpose of the invention is realized by the following technical scheme:
the heavy metal contaminated soil restoration agent is characterized by being formed by combining porous silicon and rice straw biochar, wherein the mass ratio of the porous silicon to the rice straw biochar is 1: 1-9: 1.
The particle size of the porous silicon and the rice straw biochar is less than or equal to 1 mm.
The mass ratio of the porous silicon to the rice straw biochar is 1: 1.
The mass ratio of the porous silicon to the rice straw biochar is 1.5: 1.
The mass ratio of the porous silicon to the rice straw biochar is 7: 3.
The mass ratio of the porous silicon to the rice straw biochar is 4: 1.
The mass ratio of the porous silicon to the rice straw biochar is 9: 1.
A remediation method involving any one of the heavy metal contaminated soil remediation agents, characterized in that the remediation method comprises the following steps:
(1) mixing the repairing agent and the polluted soil according to the mass ratio of 1:10, and screening fine particles with the particle size of less than 1mm for later use through a sieve with the aperture of 1 mm; the repairing agent is formed by combining porous silicon and rice straw biochar, and the mass ratio of the porous silicon to the rice straw biochar is 1: 1-9: 1;
(2) preparing a culture medium of bacteria, wherein the culture medium is prepared from yeast extract powder, ammonium sulfate and trihydroxymethyl aminotoluene; sterilizing the culture medium at high temperature; inoculating 2% of a bacterial liquid into the culture medium, wherein the bacterial liquid is bacillus pasteurianus, and the strain number is ATCC 11859; then carrying out shake culture in a constant temperature incubator for 48 h; inoculating the cultured bacterial liquid into the optimized culture medium according to the inoculation ratio of 5%, and selecting constant-temperature culture for 12h during amplification culture, wherein the light absorption value OD600 at the wavelength of 600nm is = 1.2;
(3) weighing 150g of soil sample obtained by mixing and drying the remediation agent and the polluted soil, and mixing the soil sample with the bacterial liquid with the concentration of OD600=1.2 and the volume of 40 ml;
(4) uniformly stirring the bacterial liquid and the soil sample, and then loading the mixture into a cylindrical flexible mold with the diameter of 40mm and the height of 80mm to form a cylindrical sample;
(5) fully soaking the cylindrical sample with the cylindrical flexible mold in 1L of cementing nutrient solution, and inserting an oxygen pump for oxygen pumping so as to fully generate mineralization reaction; the cementing liquid consists of NH4Cl, nutrient broth, NaHCO3, Urea and CaCl 2.2H2O;
(6) and taking out the cylindrical sample after the mineralization reaction time of 10 days is reached, removing the cylindrical flexible mold, and naturally drying.
The culture medium is formed by combining 20g/L of yeast extract powder, 10g/L of ammonium sulfate and 15.748g/L of trihydroxymethyl aminotoluene; the high-temperature sterilization of the culture medium means that the culture medium is sterilized in a high-temperature sterilization pot at 121 ℃ for 20 minutes; the temperature in the incubator was set at 30 ℃ and 200 rpm.
The concentration of each component in the cementing liquid is as follows: NH4Cl 10g/L, nutrient broth 3.0g/L, NaHCO 32.12g/L, Urea 42.0.0 g/L, CaCl 2.2H2O 88.42 g/L.
The invention has the advantages that: the repairing agent is prepared by using MICP technology, porous silicon and rice straw biochar as raw materials, so that the zinc-lead polluted soil is repaired, the leaching concentration of heavy metals in the polluted soil is reduced, the solidification/stabilization of the polluted soil is realized, and the secondary development and utilization of a polluted site are realized.
Drawings
FIG. 1 is the relationship between the leaching concentration of zinc and lead and the repairing agent in different proportions;
FIG. 2 is a graph showing the relationship between unconfined compressive strength and a healing agent in accordance with the present invention;
FIG. 3 is a graph of the leaching concentration of zinc in relation to the freeze-thaw cycle in the present invention;
FIG. 4 is a graph of lead leach concentration versus freeze-thaw cycle in accordance with the present invention;
FIG. 5 is a graph of unconfined compressive strength versus freeze-thaw cycles in accordance with the present invention.
Detailed Description
The features of the present invention and other related features are described in further detail below by way of example in conjunction with the following drawings to facilitate understanding by those skilled in the art:
example (b): as shown in fig. 1-5, the present embodiment specifically relates to a heavy metal contaminated soil remediation agent and a remediation method thereof, and the remediation method employs a remediation agent developed by using MICP technology and porous silicon and rice straw biochar as raw materials to remediate zinc-lead contaminated soil, thereby effectively solving the problem of contaminated soil solidification/stabilization; the repairing agent is formed by combining porous silicon (DKG) and rice straw biochar (SDT), wherein the mass ratio of the porous silicon to the rice straw biochar is 1: 1-9: 1; .
The porous silicon material mainly comprises six elements of C, Ca, O, Si, Mg and Al, and exists in the form of stable metal oxide of magnesium oxide and aluminum oxide. The porous silicon contains bound water inside and rich mesopores on the surface, and a large number of silicon-oxygen bonds and hydroxyl groups are distributed on the mesopores, so that the porous silicon has large specific surface area and pore volume. The surface of the porous silicon has a large number of honeycomb-shaped mesoporous structures, and silicon-oxygen bonds and hydroxyl groups are distributed in the mesopores, so that the porous silicon has good adsorption performance and can provide adsorption sites for heavy metal ions.
The pH value of the rice straw biochar is 10, the specific surface area is 21.42m2/g, the average pore diameter is 9.35nm, and the proportion of C, H, N, O is 44.5, 1.89, 1.35 and 10.35 respectively.
The MICP (Microbially induced calcium carbonate precipitation) technology is a process of generating carbonate by combining carbonate ions generated by decomposing urea with free metal cations in a cementing liquid through the urease which can decompose the urea generated by the Pasteurella in the metabolism of the Pasteurella.
As shown in fig. 1 to 5, the method for repairing heavy metal contaminated soil by using the repairing agent of heavy metal contaminated soil in the embodiment includes the following steps:
(1) preparing a repairing agent, wherein the repairing agent is formed by combining porous silicon and rice straw biochar, and 5 groups of repairing agent formulas are selected in the embodiment and are shown in the following table:
mixing a repairing agent (75 g) and heavy metal polluted soil (750 g) according to the mass ratio of 1:10, sieving the mixture through a sieve with the aperture of 1mm, and screening fine particles with the particle size of less than 1mm for later use:
when the No. 1 repairing agent is used, 37.5g of porous silicon (DKG) and 37.5g of rice straw biochar (SDT) are taken;
when the No. 2 repairing agent is used, 45g of porous silicon (DKG) and 30g of rice straw biochar (SDT) are taken;
when the No. 3 repairing agent is used, 52g of porous silicon (DKG) and 22.5g of rice straw biochar (SDT) are taken;
when the No. 4 repairing agent is used, 60g of porous silicon (DKG) and 15g of rice straw biochar (SDT) are taken;
when the No. 5 repairing agent is used, 67.5g of porous silicon (DKG) and 7.5g of rice straw biochar (SDT) are taken.
(2) Preparing a culture medium of bacteria, wherein the culture medium is prepared by a formula of 20g/L yeast extract powder, 10g/L ammonium sulfate and 15.748g/L trihydroxymethyl amino toluene (Tris); after complete dissolution, the medium was sterilized in a autoclave at 121 ℃ for 20 min.
Inoculating 2% of bacterial liquid into a culture medium, wherein the bacterial liquid is bacillus pasteurii (Sporosarcina pasteurii), and the bacterial number is as follows: ATCC11859, which is a bacterium that is non-pathogenic and can hydrolyze urea, is commercially available. The optical density of the bacterial liquid is measured by a spectrophotometer (ultraviolet (UV) -1700 ultraviolet-visible spectrophotometer) and is expressed by OD600 as the absorbance value with the wavelength of 600 nm.
Then shake culturing for 48h in a constant temperature incubator with 30 ℃ and 200rpm, inoculating the cultured bacterial liquid into an optimized culture medium according to the inoculation ratio of 5%, and selecting constant temperature culturing for 12h during amplification culture so as to keep the optimal optical density, namely OD600= 1.2.
(3) 150g of a soil sample obtained by mixing and drying a remediation agent and contaminated soil was weighed and mixed with a bacterial solution having a concentration of OD600=1.2 and a volume of 40 ml.
(4) And uniformly stirring the bacterial liquid and the soil sample, and then filling the mixture into a cylindrical flexible mold with the diameter of 40mm and the height of 80mm to form a cylindrical sample.
(5) Fully soaking a cylindrical sample with a cylindrical flexible mold in 1L of cementing nutrient solution, and inserting an oxygen pump for oxygen pumping so as to fully generate mineralization reaction; the concentrations of all substances in the cementing nutrient solution are as follows: NH4Cl 10g/L, nutrient broth 3.0g/L, NaHCO 32.12g/L, Urea 42.0.0 g/L, CaCl 2.2H2O 88.42 g/L.
(6) And taking out the cylindrical sample after the mineralization reaction time of 10 days, removing the cylindrical flexible mold, and naturally air-drying.
(7) As shown in fig. 1-5, the air-dried 5 sets of formulation samples were subjected to unconfined compressive strength test, freeze-thaw cycling test and toxicity leaching test, wherein the heavy metal content in the toxicity leaching test was determined by flame atomic absorption method.
As shown in fig. 1, which is a schematic diagram of the relationship between the leaching concentrations of zinc and lead and different proportions of the remediation agents (remediation agents No. 1, 2, 3,4 and 5) in this example, when the contaminated soil in the mining area is taken back, the heavy metal concentration is measured, and the leaching concentration of lead in the soil on the site is measured to be 7.61mg/L and the leaching concentration of zinc is measured to be 8.01 mg/L. After the above-described repairing agent and repairing method are used in combination, the leaching concentrations of zinc and lead are shown in fig. 1. It can be known from images that in the polluted soil treated by 5 groups of different repairing agents, the degradation effect of zinc is the best when the No. 1 repairing agent is adopted, the degradation rate is up to 91%, the degradation effect of lead ions is also the best, and the degradation rate is up to 96%.
As shown in fig. 2, which is a schematic diagram of the relationship between the unconfined compressive strength and the repairing agents (repairing agents 1, 2, 3,4, and 5) in different proportions in this example, it can be seen from the images that the unconfined compressive strength in the repairing agent 3 is the highest in the contaminated soil treated by 5 groups of different repairing agents, which is 506KPa, after the repairing agents and the repairing method are used in combination.
As shown in fig. 3, which is a schematic diagram of a relationship between a leaching concentration of zinc and a freeze-thaw cycle in this embodiment, after the contaminated soil is restored by 5 remediation agents with different ratios, the leaching concentration of zinc is in an ascending trend but does not greatly expand after being subjected to freeze-thaw damage, and compared with the concentration of heavy metals in the contaminated soil without treatment, the zinc degradation effect is still significant, and the degradation rate of the remediation agent No. 2 after the 7 th freeze-thaw cycle to zinc is the lowest, namely 69%; the No. 1 repairing agent has the best degradation effect, and the minimum degradation rate is as high as 80% even after 7 freeze-thaw cycles. Therefore, from the viewpoint of the degradation degree of zinc, the best degradation is the repairing agent No. 1, and the worst is the repairing agent No. 2, so the repairing agent No. 1 is the best option.
As shown in fig. 4, which is a schematic diagram of the relationship between the leaching concentration of lead and the freeze-thaw cycle in this embodiment, after the contaminated soil is repaired by 5 repairing agents with different proportions, the leaching concentration of lead is in an ascending trend but does not greatly expand after the freeze-thaw damage, and compared with the concentration of heavy metals in the contaminated soil without treatment, the degradation effect is still significant, and the degradation rate of the repairing agent No. 5 to zinc after the 7 th freeze-thaw cycle is the lowest, which is 57%; the No. 1 repairing agent has the best degradation effect, and the minimum degradation rate is up to 87 percent even after 7 freeze-thaw cycles. Therefore, from the viewpoint of the degradation degree of zinc, the best degradation is the repairing agent No. 1, and the worst is the repairing agent No. 5, so the repairing agent No. 1 is the best option.
As shown in fig. 5, which is a schematic diagram of a relationship between unconfined compressive strength and freeze-thaw cycle in this embodiment, after the contaminated soil is restored by 5 remediation agents with different ratios, the strength of the test piece is in a whole descending trend after being damaged by freeze-thaw. The compressive strength of the sample repaired by the No. 3 repairing agent is best, and can reach 514KPa at most. After 7 days of freezing and thawing cycle, the unconfined compressive strength of the material is reduced to 371 KPa. The minimum strength of the solid waste after the solidification/stabilization repair of the refuse landfill is regulated by the American environmental protection agency is 350 KPa.
Compared with the No. 1 repairing agent and the No. 3 repairing agent in terms of unconfined compressive strength, although the strength of a test piece repaired by the No. 1 repairing agent is 477KPa and is reduced to 321KPa after 7 times of freeze-thaw cycle, which is not as high as that of the No. 3 repairing agent, the repairing effect shown by the No. 1 repairing agent is most ideal in terms of the leaching concentration of zinc and lead.
In this example, each test was performed as follows:
the unconfined compressive strength test adopts a full-automatic testing machine of Shandongda testing instrument, and the loading is carried out at a loading rate of 0.5 KN/s.
And in a freeze-thaw cycle test, the maintained test piece is placed into a freeze-thaw tester with the temperature set to be 20 ℃ below zero and 20 ℃ above zero, the control time of each temperature is 12 hours, and the freeze-thaw cycle for completing one period is 24 hours.
Toxicity Leaching test the concentration of leached metals in the leachate was determined using 1311-Toxicity Characteristic Leaching (TCLP) as specified by the Environmental Protection Agency (EPA). 5.7ml of glacial acetic acid is dissolved in 500ml of deionized water, and 64.3ml of 1mol/L sodium hydroxide is added to the solution to make the volume of the solution equal to 1L. The pH of the solution was adjusted to within 4.93. + -. 0.05 with 1mol/L HNO3 or NaON. The prepared solution is TCLP extract. Grinding the tested sample, sieving with 2mm sieve, adding 10ml of extractive solution and 0.5g of sediment into 50ml of centrifugal tube, covering with bottle cap, fixing on oscillator, oscillating for 18h, standing, collecting supernatant, filtering with 0.45 μm filter membrane, and testing the leaching concentration of heavy metal by flame atomic absorption method.
The beneficial effect of this embodiment lies in: compared with the method for singly using biochar to stabilize the heavy metals in the compound contaminated soil and singly using the MICP technology to solidify/stabilize the compound contaminated soil, the method for restoring the heavy metal contaminated soil has excellent effect, combines a small amount of externally-doped restoring agent with long-term beneficial chemical fixation with the microorganism in-situ restoring technology, can greatly reduce the heavy metals in the contaminated soil, simultaneously improves the strength of a contaminated soil field, and is beneficial to secondary development and utilization of the contaminated field.
Claims (10)
1. The heavy metal contaminated soil restoration agent is characterized by being formed by combining porous silicon and rice straw biochar, wherein the mass ratio of the porous silicon to the rice straw biochar is 1: 1-9: 1.
2. The heavy metal contaminated soil remediation agent of claim 1, wherein the particle size of the porous silicon and the rice straw biochar is less than or equal to 1 mm.
3. The heavy metal contaminated soil remediation agent of claim 1, wherein the mass ratio of the porous silicon to the rice straw biochar is 1: 1.
4. The heavy metal contaminated soil remediation agent of claim 1, wherein the mass ratio of the porous silicon to the rice straw biochar is 1.5: 1.
5. The heavy metal contaminated soil remediation agent of claim 1, wherein the mass ratio of the porous silicon to the rice straw biochar is 7: 3.
6. The heavy metal contaminated soil remediation agent of claim 1, wherein the mass ratio of the porous silicon to the rice straw biochar is 4: 1.
7. The heavy metal contaminated soil remediation agent of claim 1, wherein the mass ratio of the porous silicon to the rice straw biochar is 9: 1.
8. A remediation method involving the heavy metal contaminated soil remediation agent according to any one of claims 1 to 7, wherein the remediation method comprises the steps of:
(1) mixing the repairing agent and the polluted soil according to the mass ratio of 1:10, and screening fine particles with the particle size of less than 1mm for later use through a sieve with the aperture of 1 mm; the repairing agent is formed by combining porous silicon and rice straw biochar, and the mass ratio of the porous silicon to the rice straw biochar is 1: 1-9: 1;
(2) preparing a culture medium of bacteria, wherein the culture medium is prepared from yeast extract powder, ammonium sulfate and trihydroxymethyl aminotoluene; sterilizing the culture medium at high temperature; inoculating 2% of a bacterial liquid into the culture medium, wherein the bacterial liquid is bacillus pasteurii, and the strain number is ATCC 11859; then carrying out shake culture in a constant temperature incubator for 48 h; inoculating the cultured bacterial liquid into the optimized culture medium according to the inoculation ratio of 5%, and selecting constant-temperature culture for 12h during amplification culture, wherein the light absorption value OD600 at the wavelength of 600nm is = 1.2;
(3) weighing 150g of soil sample obtained by mixing and drying the remediation agent and the polluted soil, and mixing the soil sample with the bacterial liquid with the concentration of OD600=1.2 and the volume of 40 ml;
(4) uniformly stirring the bacterial liquid and the soil sample, and then loading the mixture into a cylindrical flexible mold to form a cylindrical sample;
(5) fully soaking the cylindrical sample with the cylindrical flexible mold in 1L of cemented nutrient solution, and inserting an oxygen pump for oxygen pumping so as to fully generate mineralization reaction; the cementing liquid consists of NH4Cl, nutrient broth, NaHCO3, Urea and CaCl 2.2H2O;
(6) and taking out the cylindrical sample after the mineralization reaction time of 10 days is reached, removing the cylindrical flexible mold, and naturally drying.
9. The method according to claim 9, wherein the culture medium comprises 20g/L yeast extract powder, 10g/L ammonium sulfate and 15.748g/L trihydroxymethyl aminotoluene; the high-temperature sterilization of the culture medium means that the culture medium is sterilized in a high-temperature sterilization pot at 121 ℃ for 20 minutes; the temperature in the incubator was set at 30 ℃ and 200 rpm.
10. The method for remediating heavy metal contaminated soil as recited in claim 9, wherein the concentration of each component in the cementing liquid is as follows: NH4Cl 10g/L, nutrient broth 3.0g/L, NaHCO 32.12g/L, Urea 42.0.0 g/L, CaCl 2.2H2O 88.42 g/L.
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