CN114938814B - Use of surfactant and composition thereof in promoting plant root growth - Google Patents

Use of surfactant and composition thereof in promoting plant root growth Download PDF

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CN114938814B
CN114938814B CN202210614568.7A CN202210614568A CN114938814B CN 114938814 B CN114938814 B CN 114938814B CN 202210614568 A CN202210614568 A CN 202210614568A CN 114938814 B CN114938814 B CN 114938814B
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root growth
growth
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CN114938814A (en
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刘海玉
韦能春
孙哲
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Shandong Jingbo Agrochemical Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Fertilizers (AREA)

Abstract

The invention relates to application of a surfactant and a composition thereof in promoting plant root growth, belonging to the technical field of biological stimulation. The surfactant comprises a surfactant with a fatty acyl length of C12-C16. The invention prepares the surface active agent or the composition containing the surface active agent into liquid post-treatment soil or plant body. The invention provides a novel application of a surfactant and a composition thereof in promoting plant root growth. Experiments prove that the surfactant and the composition thereof have remarkable improvement effect on root fresh weight and yield of crops such as tomatoes, wheat, melons and the like, and can be applied to agricultural production.

Description

Use of surfactant and composition thereof in promoting plant root growth
Technical Field
The invention belongs to the technical field of biostimulant, and particularly relates to application of a surfactant and a composition thereof in promoting plant root growth.
Background
The surfactant (surfactin) is a type of cyclic lipopeptide type biosurfactant, has good surface/interfacial activity, and is one of the biosurfactants with the highest research and the strongest surface activity. The surfactant was first found in bacillus subtilis broth, and then it was identified in bacillus amyloliquefaciens, bacillus beliae, and other various bacillus fermentation broths. The structure is that a heptapeptide is connected with beta-hydroxyl of fatty acid chain carbon atom through an internal ester bond. It has now been found that the majority of the surfactants which are widely present in fermentation broths are C 13 、C 14 、C 15 、C 16 A mixture of lipopeptides of four different length fatty chains, the hydrophobic amino acid residues in the peptide ring are typically located at positions 2, 3, 4, 6 and 7, while the glutamic acid and aspartic acid residues are located at positions 1 and 5, respectively. In addition, the scholars found that the number of carbon atoms of beta-hydroxy fatty acid in the surfactant structure is 12-17, and the L-Leu at the C end can be replaced by L-Ile and L-Val. The research shows that the surfactant has antibacterial, antiviral, antimycoplasma and homoptera or coleopteran preventing and treating effectsBiological activity of insects of order, and the like. In addition, the surfactant can induce plants to generate immune response, and improve the resistance of grape to downy mildew. However, to date, no study has been made to evaluate the effect of root application of high purity surfactant (95% or more) on crop root growth and yield of agricultural products.
Good root system is the basis for plants to absorb nutrition, maintain growth and ensure the yield of agricultural products. Therefore, finding active substances with a significant rooting-promoting capacity is one of the important research and development directions. Plant growth regulators (domestic pesticide management) such as indolebutyric acid, diethyl aminoethyl hexanoate and triacontanol have a certain rooting promoting effect, but the physiological activities of the substances are closely related to the dosage, the use time and the weather conditions, and obvious phytotoxicity is often caused by improper use. Humic acid, alginic acid, fish protein and other biological hormones have a certain rooting function, but they usually contain tens or even hundreds of components, and the activity of the product is greatly influenced by raw materials and processes. There is no report on the use of surfactants to promote root growth.
Disclosure of Invention
In view of the above problems in the prior art, the present invention provides a use of a surfactant and a composition thereof for promoting plant root growth, so as to solve the above problems. The lipopeptid of the surfactant family has definite structure and is easy to biodegrade, can inhibit the occurrence of partial root diseases, and is suitable for being developed into novel root-promoting and yield-increasing biological hormone.
The technical scheme of the invention is as follows:
use of a surfactant comprising a fatty acyl group of length C for promoting plant root growth 12 -C 16 Is a surfactant of (a).
The surfactant comprises fatty acyl with length of C 12 -C 16 The surfactants of (2) are respectively named C 12 -surfactant, C 13 -surfactant, C 14 -surfactant, C 15 -surfactant, C 16 -a surfactant.
As a specific embodiment, the plant comprises tomato, capsicum, cucumber, melon, wheat, rice, maize, apple, banana, citrus, peach, pear, grape.
In one embodiment, the surfactant is applied by preparing the surfactant into a liquid to post-treat soil or plant body.
As a specific embodiment, the mass concentration of the surfactant in the liquid is 1.0-900 mg/L.
Use of a composition comprising said surfactant, said composition comprising said surfactant and a chitosan oligosaccharide, for promoting plant root growth.
As a specific embodiment, the chitosan oligosaccharide is a polymer with a deacetylation degree of 75-90% and a polymerization degree of 6-20 (purity is more than or equal to 85%), and is obtained by degrading chitosan by an enzymatic method or a chemical method or a physical method.
As a specific embodiment, the plant comprises tomato, capsicum, cucumber, melon, wheat, rice, maize, apple, banana, citrus, peach, pear, grape.
As a specific embodiment, the composition is applied by formulating the composition as a liquid for post-treatment of soil or plant matter.
As a specific embodiment, the mass concentration of the surfactant in the liquid is 1.0-900 mg/L.
In one specific embodiment, the mass ratio of the surfactant to the chitosan oligosaccharide in the surfactant-containing composition is 3.5-25.0:1.0.
The beneficial effects of the invention are as follows:
the invention provides a novel application of a surfactant and a composition thereof in promoting plant root growth. Experiments prove that the surfactant and the composition thereof have remarkable improvement effect on root fresh weight and yield of crops such as tomatoes, wheat, melons and the like, and can be applied to agricultural production.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the description of the embodiments or the prior art will be briefly described below, and it will be obvious to those skilled in the art that other drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a graph showing the comparison of the UPLC profile of the surfactant prepared in example 1 of the present invention with a surfactant standard; wherein the upper panel shows a surfactant standard and the lower panel shows the surfactant prepared in example 1.
FIG. 2 is a C prepared in example 1 of the present invention 12 -MS profile of surfactant.
FIG. 3 is a C prepared in example 1 of the present invention 13 -MS profile of surfactant.
FIG. 4 is a C prepared in example 1 of the present invention 14 -MS profile of surfactant.
FIG. 5 is a C prepared in example 1 of the present invention 15 -MS profile of surfactant.
FIG. 6 is a C prepared in example 1 of the present invention 16 -MS profile of surfactant.
FIG. 7 is a graph showing comparison of tomato root growth in example 2 of the present invention.
FIG. 8 is a graph showing comparison of melon seedling growth in example 3 of the present invention.
FIG. 9 is a comparative graph of wheat growth in example 4 of the present invention.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Example 1
Preparation of surfactant
(1) Bacillus subtilis ATCC21332 was subjected to expansion culture to obtain a fermentation broth.
(2) And centrifuging the fermentation liquor to remove thalli, and obtaining supernatant.
(3) Concentrating the supernatant by using a rotary evaporator for 3 times, regulating the pH value of the feed liquid to 6.5 by using 4mol/L sulfuric acid, then adding 95% (V/V) ethanol with equal volume into the concentrated liquid, oscillating to fully mix the feed liquid, enabling the protein in the concentrated liquid to denature to form floccules by using the ethanol, and centrifuging to remove floccules protein to obtain the supernatant.
(4) The clear liquid is subjected to ethanol removal by a rotary evaporator, and then the pH value of the feed liquid is regulated to 2.0 by using 4mol/L sulfuric acid so as to precipitate and separate out the surfactant. And (3) collecting the precipitate after centrifugation, wherein the impurity in part of fermentation liquor is remained in the product, and the precipitate is subjected to 2 times of acid washing by adopting sulfuric acid aqueous solution with pH of 2, and the precipitate is collected after centrifugation and freeze-dried to obtain a surfactant sample.
(5) The surfactant samples were qualitatively analyzed using UPLC-MS, while outsourced surfactant standards (purchased from Sigma) were used as controls, and the comparison results are shown in FIG. 1. The top panel in FIG. 1 shows the surfactant standard and the bottom panel shows the surfactant prepared in example 1. As can be seen by comparison, the standard contains four peaks, each having a fatty acyl length of C 12 ～C 15 Is a surfactant of (a). The peak position of the surfactant prepared in example 1 is consistent with that of a standard substance; the peak at rt=12.6 min was increased to a fatty acyl length C 16 Is a surfactant of (a). And determining the surfactant and the homolog thereof contained in the detection sample by a full scanning mode.
The MS spectra of the surfactant samples are shown in figures 2-6.
Mass spectrometry detection results of the purified samples are shown in table 1 below:
TABLE 1 Mass Spectrometry detection results for samples
Serial number of the compound m/z[M-H]- Identification result
1 992.6310 C 12 -a surfactant
2 1006.6455 C 13 -a surfactant
3 1020.6612 C 14 -a surfactant
4 1034.6779 C 15 -a surfactant
5 1052.6875 C 16 -a surfactant
Example 2
Use of surfactant for promoting growth and increasing yield of tomato root system
The test was performed in 2020 in glass greenhouse from Jingbong chemical technology Co., ltd. And the test material was cherry tomato. Turfy soil (purchased in the flower market) and sand were mixed according to a ratio of 1:1, uniformly mixing and loading the mixture into a seedling tray with the thickness of 54cm multiplied by 27cm multiplied by 6cm, uniformly sowing seeds into the seedling tray, covering a soil layer with the thickness of about 0.5cm, watering from the bottom, and sprouting and raising the seedlings under the condition of abundant sunlight at 24-28 ℃. Selecting the tomato plants after growing to 6-8 leavesTransplanting the seedlings with the same vigor into a flowerpot (the diameter of the flowerpot is 30cm, the height of the flowerpot is 15cm, each pot is filled with 9.0kg of field soil), keeping the growth conditions of each pot to be consistent, and applying a base fertilizer after seedling reviving, wherein the base fertilizer is a compound fertilizer (N-P) 2 O 5 -K 2 O=15-15-15), in an amount of 500kg/hm 2 And (3) application.
The experiment was set up with 3 treatments, 30 seedlings per treatment, 10 seedlings in one group, three replicates. (1) treatment group A was a blank control, and water-soluble fertilizer was applied. 20-20-20 (N-P) 2 O 5 -K 2 O) diluting the water-soluble fertilizer 300 times, and applying 100mL of the water-soluble fertilizer diluent to the root of each tomato seedling. (2) Treatment group B was administered with potassium fulvate (commercially available, 50% humic acid content). To 3000mL of 20-20-20 (N-P) 2 O 5 -K 2 And O) adding 10g of ore source potassium fulvate into the 300-time dilution of the water-soluble fertilizer, stirring to completely dissolve the ore source potassium fulvate to obtain a humic acid-containing water-soluble fertilizer solution, and applying 100mL of the solution to the root of each tomato seedling. (3) treatment group C the surfactant prepared in example 1 was applied. To 3000mL of 20-20-20 (N-P) 2 O 5 -K 2 And O) adding 0.30g of surfactant into the 300-time dilution of the water-soluble fertilizer, stirring to completely dissolve the surfactant to obtain a water-soluble fertilizer solution with the surfactant content of 100mg/L, and applying 100mL of the solution to the root of each tomato seedling.
And after 20 days of the water-soluble fertilizer application, 6 plants are randomly selected for each treatment group, root soil is carefully washed away, and the fresh weight of roots and the fresh weight on the ground are measured. 3 plants were additionally taken for each treatment, the root system was washed in an ice-water bath and 0.5g of fresh root tip was taken and the root system activity of the different treatment groups was determined by TTC staining. Root growth pairs such as shown in fig. 7, the specific results are shown in table 2 below:
TABLE 2 detection results
Treatment group Fresh root weight (g) Root system activity (mg. G) -1 ·h -1 ) Fresh weight on the ground (g)
A 128.6 7.3 197.9
B 158.1 9.8 237.4
C 240.7 12.5 302.3
As can be seen from the data in table 2 and fig. 7, the surfactant can strongly stimulate the growth of the root system of the tomato, the fresh weight of the root is improved by 87.2% and 52.2% respectively compared with the control group (treatment a) and the humic acid treatment group (treatment B), and the root system maintains higher activity. The good root system enhances the nutrient absorption capacity of plants, thereby promoting the accumulation of overground biomass; the fresh weight of the surface active agent treated group is improved by 52.8 percent and 27.3 percent respectively compared with the control group and the humic acid treated group.
The remaining 21 tomatoes in each treatment group continue to grow, and the water and fertilizer application amounts and application time are kept completely consistent. The treatment group B and the treatment group C are respectively applied with the water-soluble fertilizer containing humic acid and surfactant once in the young fruit period (the dosage of the humic acid and the surfactant is the same as the dosage), and the treatment group A is only applied with the water-soluble fertilizer with the same dosage. And the tomato yield and quality were investigated during the fruiting period (four batches of fruits before production). The results are shown in Table 3 below:
TABLE 3 results of tomato yield and quality index for different treatment groups
a The higher the solid to acid ratio, the sweeter the fruit. b The higher the vitamin C content, the better the fruit quality.
The data in Table 3 shows that the tomato yield of the surfactant treated group was 22.9% and 11.5% higher than that of the treated group A, B, respectively. The quality of the tomatoes in the surfactant treatment group is obviously improved compared with the tomatoes in the other two groups, and the mouthfeel is obviously improved. Tomato yield and quality data are positively correlated with root growth conditions, which are highly consistent with a fact observed for a long time in agricultural production, i.e. good root systems are the basis for healthy growth and high yield of crops.
Example 3
Use of surfactant and composition thereof in promoting rooting of melons
The test was performed in 2021 in glass greenhouse from genitourism technology limited, the test material was melon (boyang No. 9). Uniformly mixing turfy soil and sand according to a ratio of 1:1, loading the mixture into a 54cm multiplied by 27cm multiplied by 6cm seedling raising tray, uniformly sowing seeds into the seedling raising tray, covering a soil layer with a thickness of about 0.5cm, watering from the bottom, and sprouting and raising seedlings of the seeds under the condition of abundant sunlight at 24-28 ℃. After melon plants grow to 2-4 leaves, selecting seedlings with consistent growth vigor, and transplanting the seedlings into flowerpots (the diameter of each flowerpot is 15cm, the height of each flowerpot is 11cm, and each pot is filled with 1.0kg of field soil). The pH value of the soil is 7.36, and the contents of organic matters, alkaline hydrolysis nitrogen, quick-acting phosphorus and quick-acting potassium are 25.14g/kg, 135.71mg/kg, 32.25mg/kg and 121.29mg/kg respectively.
The test was run with a total of 7 treatments, 30 seedlings per treatment, 10 seedlings in one group, and three replicates. 5 days after transplanting, the root is applied with Beijing bo Zhilsesquih water-soluble fertilizer and different biological stimulation hormones. Wherein D is a control group, only water-soluble fertilizer diluent is applied, and the components of the water-soluble fertilizer diluent are 20-20-20 (N-P) 2 O 5 -K 2 The water-soluble fertilizer of O) mainly comprises monoammonium phosphate, urea and potassium sulfate. E-J is prepared by respectively adding different biological hormones into water-soluble fertilizer diluentThe experimental design is specifically shown in table 4:
TABLE 4 test design
According to the design of Table 4, 6 plants were randomly selected for each treatment group 15 days after application of the above water-soluble fertilizer, root soil was carefully washed off, and root fresh weight and ground fresh weight were measured. 3 plants were additionally taken for each treatment, the root system was washed in an ice-water bath and 0.5g of fresh root tip was taken and the root system activity of the different treatment groups was determined by TTC staining. The melon growth pairs for the different treatment groups are shown in fig. 8, and the results are shown in table 5 below:
TABLE 5 comparison of root systems and growth indicators of melons from different treatment groups
Treatment group Average root fresh weight (g) Root system activity (mg. G) -1 ·h -1 ) Fresh weight on the ground (g)
D 2.85 5.73 5.67
E 3.76 7.52 6.61
F 4.65 9.32 8.62
G 3.94 8.11 7.51
H 5.46 10.87 11.82
I 3.47 6.33 5.72
J 3.75 6.96 6.38
The data in table 5 shows that: (1) Compared with the control group, the surfactant, the chitosan oligosaccharide and the humic acid can obviously promote the growth of root systems and overground parts of melon seedlings and enhance the activity of the root systems, so that the surfactant has the best performance. (2) The combination ratio of the surfactant and the chitosan oligosaccharide is 10:1, and the surfactant and the chitosan oligosaccharide have obvious synergistic effect; when the chitosan oligosaccharide proportion in the composition is higher, a certain antagonism effect appears on the chitosan oligosaccharide and the chitosan oligosaccharide. (3) The combination of surfactant with humic acid (50 mg/L+1617mg/L) showed a remarkable antagonistic effect.
Example 4
Use of surfactant in increasing wheat yield
The test was carried out in 2020 at the Pongjia laboratory base of Jingbnong chemical technology Co., ltd. For a period of time of 10 months in 2020 to 6 months in 2021. The fertilization and soil preparation before sowing are carried out according to the conventional operation. The pH value of the soil is 7.24, and the contents of organic matters, alkaline hydrolysis nitrogen, quick-acting phosphorus and quick-acting potassium are 16.33g/kg, 145.12mg/kg, 37.67mg/kg and 131.57mg/kg respectively. The wheat variety is Jimai 22, the sowing quantity is 10 kg/mu, and the sowing area is 10 mu.
Two treatment groups (5 mu each) were set for the trial: control group (treatment group K) was routinely managed; the experimental group (treatment group L) sprays 50mg/L of surfactant on the basis of conventional management in the main leaf stage and booting stage of wheat respectively. The fresh weight (green-turning period), tiller number and second internode length of the wheat root are tracked and measured, and the yield of the wheat of the two treatment groups is measured according to DB 32/T2856-2015. The pairs of ear growths for the different treatment groups are shown in fig. 9, with the results shown in table 6 below:
TABLE 6 comparison of wheat growth index and yield for different treatment groups
As can be seen from the data in fig. 9 and table 6, spraying the surfactant can promote tillering of wheat, shortening the second internode length (helping to reduce lodging). The theoretical yield of the wheat in the experimental group is increased by 15.2% compared with that in the control group.
Although the present invention has been described in detail by way of preferred embodiments with reference to the accompanying drawings, the present invention is not limited thereto. Various equivalent modifications and substitutions may be made in the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and it is intended that all such modifications and substitutions be within the scope of the present invention/be within the scope of the present invention as defined by the appended claims. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (4)

1. Use of a surfactant-containing composition for promoting plant root growth, wherein the composition comprises the following components in parts by weight: 45 parts of surfactant and 5 parts of chitosan oligosaccharide; the chitosan oligosaccharide is a polymer with the deacetylation degree of 75-90% and the polymerization degree of 6-20.
2. The use according to claim 1, wherein the plant comprises tomatoes, peppers, cucumbers, melons, wheat, rice, corn, apples, bananas, citrus fruits, peaches, pears, grapes.
3. The use according to claim 1, wherein the composition is applied by formulating the composition as a liquid for the post-treatment of soil or plant matter.
4. The use according to claim 3, wherein the mass concentration of the surfactant in the liquid is 1.0 to 900mg/L.
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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112299909A (en) * 2020-11-02 2021-02-02 河南荣莉莱智慧农业发展有限公司 Fly-prevention plant protection compound agent for wheat in mature period

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102391876B (en) * 2011-09-29 2015-03-04 山东靠山生物科技有限公司 Composite biological soil modifier and application thereof
US9737572B2 (en) * 2012-07-30 2017-08-22 Core Intellectual Properties Holdings, Llc Methods and compositions of biocontrol of plant pathogens
CN104904751B (en) * 2015-04-09 2018-03-06 河南工业大学 A kind of wheat scab liquid biocontrol agent and preparation method thereof
FR3061410B1 (en) * 2016-12-30 2020-07-10 Lipofabrik BIOSTIMULANT COMPOSITION OF PLANT GROWTH OBTAINED FROM SURNANTANT OF BACILLUS STRAIN CULTURE SP.
CN112646739B (en) * 2020-11-25 2021-11-19 山东碧蓝生物科技有限公司 Bacillus and application thereof
CN112794746B (en) * 2021-03-16 2022-09-02 河南新仰韶生物科技有限公司 Composite bacterial powder for Chinese yam and preparation method thereof, and composite bacterial fertilizer for Chinese yam and preparation method thereof
CN113142213B (en) * 2021-04-25 2022-02-18 河北省科学院生物研究所 Application of surfactin family lipopeptide in pest control

Patent Citations (1)

* Cited by examiner, † Cited by third party
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