CN114934078A - Method for promoting synthesis of carbon source in homoacetogenesis process through large-scale sludge acclimation - Google Patents
Method for promoting synthesis of carbon source in homoacetogenesis process through large-scale sludge acclimation Download PDFInfo
- Publication number
- CN114934078A CN114934078A CN202210450992.2A CN202210450992A CN114934078A CN 114934078 A CN114934078 A CN 114934078A CN 202210450992 A CN202210450992 A CN 202210450992A CN 114934078 A CN114934078 A CN 114934078A
- Authority
- CN
- China
- Prior art keywords
- sludge
- carbon source
- homoacetogenic
- anaerobic
- treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000010802 sludge Substances 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 47
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 28
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 17
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 17
- 230000001737 promoting effect Effects 0.000 title claims abstract description 9
- 230000003403 homoacetogenic effect Effects 0.000 claims abstract description 28
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 23
- 241000894006 Bacteria Species 0.000 claims abstract description 17
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 12
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 11
- 239000001257 hydrogen Substances 0.000 claims abstract description 11
- 238000012163 sequencing technique Methods 0.000 claims abstract description 9
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 6
- 230000007613 environmental effect Effects 0.000 claims abstract description 4
- 239000010865 sewage Substances 0.000 claims abstract description 4
- 239000002253 acid Substances 0.000 claims abstract description 3
- 239000002994 raw material Substances 0.000 claims abstract description 3
- 230000004151 fermentation Effects 0.000 claims description 20
- 208000014530 benign exophthalmos syndrome Diseases 0.000 claims description 17
- 239000007789 gas Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000010306 acid treatment Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 238000000967 suction filtration Methods 0.000 claims description 3
- 239000003513 alkali Substances 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 230000029219 regulation of pH Effects 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 230000002194 synthesizing effect Effects 0.000 abstract description 2
- 241001074903 Methanobacteria Species 0.000 abstract 1
- 230000002053 acidogenic effect Effects 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 238000004519 manufacturing process Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 239000002002 slurry Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 230000000789 acetogenic effect Effects 0.000 description 3
- 230000001651 autotrophic effect Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 229940100228 acetyl coenzyme a Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000011085 pressure filtration Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/54—Acetic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Treatment Of Sludge (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention provides a method for promoting synthesis of a carbon source in a homoacetogenic process by large-scale sludge domestication, which comprises the following steps: anaerobic sludge of a sewage plant is taken as a raw material, non-acidogenic strains such as methanogens and the like in the sludge are inhibited through pretreatment, and a process of synthesizing a carbon source by acclimatizing and enriching homoacetogenic bacteria in an anaerobic fermentation tank in a later-stage environment control manner is carried out; when the environmental conditions are that the air pressure is between 0 and 5bar, the ratio of hydrogen to carbon dioxide is between 8:1 and 1:1, the temperature is between 25 and 50 ℃, the subsequent recovery of methanobacteria is avoided by controlling the pH to be below 5.5, the sequencing time is controlled to be between 7 and 30 days, and the carbon source yield can be kept between 15 and 40 mgCOD/L/h. The method has simple process, avoids complicated multiple treatment processes to inhibit methane bacteria through simple pH regulation, gradually acclimates homoacetogenic bacteria through gradient regulation of pH, realizes enrichment of acid-resistant homoacetogenic bacteria, thereby realizing economic balance and technical feasibility in the process and being beneficial to engineering development.
Description
Technical Field
The invention relates to the technical fields of microbial fermentation technology and environment-friendly purification treatment, in particular to a method for promoting synthesis of a carbon source in a homoacetogenic process through large-scale sludge acclimation.
Background
The anaerobic fermentation process mainly comprises three stages of hydrolysis acidification, acetic acid production and methane production, and finally, the process of converting organic matters in the wastewater into methane. In the acetogenic stage, the production of acetic acid mainly depends on the action of hydrogen-producing acetogenic bacteria, and in addition, a small amount of acetic acid is produced by utilizing CO from homoacetogenic bacteria 2 And H 2 And (4) synthesizing.
Homoacetogenic bacteria are a kind of bacteria which can utilize various organic substrates to grow heterotrophically and can also utilize CO 2 And H 2 Or anaerobic microorganisms for CO autotrophic survival have stronger ecological environment adaptability. Under anaerobic conditions, homoacetogenic bacteria can convert different substrates into organic matters such as acetic acid, propionic acid, ethanol and the like through an acetyl coenzyme A way. The organic matters are mainly degradable organic matters and can be used as a supplementary carbon source to be added in the denitrification process, so that the C/N ratio requirement of denitrification is met, and the smooth proceeding of the reaction is ensured. However, in the traditional anaerobic fermentation process, the hydrogen partial pressure in the system is low, the homoacetogenic process is obviously limited, and the hydrogen is only used as a hydrogen consumption way to keep the hydrogen balance in the system.
In the strategic context of "carbon peak and carbon neutralization", homoacetogens absorb CO 2 The ability to perform autotrophic production is of great interest. In the prior art, for example, in the homoacetogenic bacteria enriched sludge anaerobic high-efficiency acetogenic process with application publication number of CN201410467411.1, molasses waste water is required to be matched as a substrate, methanogen activity is inhibited through initial pH value adjustment, and hydrogen-producing acetogens and homoacetogenic bacteria activity are improved. However, the current research is still mainly directed to the culture of pure strains or the research on laboratory scale, and has the problems of difficult culture, high cost and the like, which is not favorable for engineering application. Therefore, a method suitable for large-scale anaerobic sludge enrichment culture of homoacetogenic bacteria is needed.
Disclosure of Invention
In order to overcome the defects and shortcomings of the prior art, the invention aims to provide a method for promoting the synthesis of a carbon source in the process of homoacetogenesis through large-scale sludge acclimation. The method promotes the homoacetogenic process by inhibiting methanogens in the early stage, and realizes the enrichment of homoacetogenic bacteria and the continuous synthesis of the produced carbon source by gradually regulating and controlling the pH in the later stage.
In order to achieve the above purpose, the invention provides the following technical scheme:
a method for promoting synthesis of a carbon source in a homoacetogenic process by large-scale sludge domestication comprises the following steps:
1) anaerobic sludge of a sewage plant is taken as a raw material, the anaerobic sludge is washed by clear water, and non-acid-producing strains such as methanogens and the like in the sludge are inhibited through pretreatment;
2) placing the anaerobic sludge obtained in the step 1) in a fermentation tank, introducing a mixed gas of hydrogen and carbon dioxide into the fermentation tank, and continuously fermenting at the temperature of 25-50 ℃; controlling the pH value to be kept stable in the fermentation process, wherein the stable pH value is between 4.5 and 7.0, and the pH value is controlled to be 7.0 in an initial sequencing batch mode;
3) taking out the sludge used in the step 2) after 7-30 days of the sequencing batch, and separating to obtain an aqueous solution containing a carbon source and sludge enriched with homoacetogenic bacteria;
4) the sludge can be subjected to the steps 2) and 3) again, wherein the stable pH value is reduced by 0.5 compared with the previous sequence in batches until the stable pH value is 4.5 and then is kept stable, so that the recovery of subsequent methane bacteria is avoided, and the system can be ensured to inhibit the generation of methane and keep the system to stably output carbon sources without other treatment conditions.
In the scheme of the invention, the anaerobic sludge in the step 1) is any one of anaerobic granular sludge or anaerobic flocculent sludge.
In a further preferred embodiment of the present invention, the sludge is anaerobic flocculent sludge; the sludge concentration is between 5 and 60 gMLSS/L.
In the embodiment of the present invention, the pretreatment manner in step 1) is one or more of heat treatment at 105 ℃, acid treatment at pH 3.0, alkali treatment at pH 11, and 0-100mM/L BES treatment, wherein the preferred pretreatment manner is acid treatment at pH 3.0, 0-100mM/LBES treatment, and 0-100mM/L BES treatment at pH 3.0, and most preferred is addition of 0-100mM/L BES treatment at pH 3.0.
In a more preferred embodiment of the invention, the treatment time is 0 to 72 hours, wherein the treatment time is preferably 24 to 48 hours.
In the scheme of the invention, the BES is added in an amount of 0-100mM/L, preferably 50 mM/L; the amount of BES added in the concurrent BES treatment at pH 3.0 is 0 to 100mM/L, preferably 25 mM/L.
In the scheme of the invention, the fermentation environment in the step 2) is an anaerobic fermentation environment, the anaerobic atmosphere is a mixed atmosphere of hydrogen and carbon dioxide, and the air pressure is kept between 0 and 2 bar.
In the scheme of the invention, H in the atmosphere 2 :CO 2 H is preferably selected from 8:1 to 1:1(v: v) 2 :CO 2 ＝4:1。
In the scheme of the invention, the environmental temperature is controlled to be between 25 ℃ and 50 ℃ in the fermentation process in the step 2), and the preferable temperature is 37 ℃.
In the scheme of the invention, the sequencing batch time in the step 3) is 7-30 days, wherein the preferred sequencing batch time is 15 days.
In the scheme of the invention, the mud-water separation mode in the step 3) is one or more of centrifugation, natural sedimentation, filter pressing and suction filtration.
Compared with the prior art, the invention has the beneficial effects that:
(1) by domesticating and culturing the anaerobic sludge, the problems of high operation difficulty and high cost of pure strain culture are solved.
(2) The directional enrichment of low-pH tolerance homoacetogenic bacteria is carried out by regulating and controlling the pH in the later period, the long-term inhibition of methane is realized, the repeated use of inhibiting means such as an inhibitor and heat treatment is avoided, the running cost of the device is reduced, and the continuous culture is realized.
(3) The pure autotrophic process is carried out by utilizing the culture sludge to realize CO 2 The recycling and resource utilization of the carbon dioxide provide a new idea for realizing the carbon reduction target of the sewage treatment plant.
Detailed Description
The present invention will be further explained with reference to examples. The following examples are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.
Example 1
Cleaning anaerobic granular sludge of 20g/L with clear water for 3 times, and pretreating at 105 deg.C for 12 h. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture is 2bar, the gas proportion is H 2 :CO 2 The culture was carried out at a rate of 8:1 and maintained at 25 ℃ and a pH of 7.0 to 4.5 for a 15-day sequencing batch period, and the slurry was separated from the water by natural sedimentation.
The COD production rate of the sludge finally obtained by enrichment under the condition of pH 4.5 is about 15.3mgCOD/L/h, and no methane is generated after 90 days of reaction.
Example 2
5g/L anaerobic flocculent sludge is washed by clear water for 3 times and then pretreated for 48 hours under the condition that the pH value is 3.0. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture is up to 3bar, the gas proportion is H 2 :CO 2 Culturing at 50 deg.C and pH 7.0-4.5 at 1:1 for 12 days, and separating mud and water by pressure filtration.
The COD production rate of the sludge finally obtained by enrichment under the condition of pH 4.5 is about 14.5mgCOD/L/h, and no methane is generated after 90 days of reaction.
Example 3
The anaerobic flocculent sludge with the concentration of 10g/L is washed for 3 times by clear water and then pretreated for 72 hours under the condition that the pH value is 11.0. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture is up to 1bar, the gas proportion is H 2 :CO 2 The culture was carried out at 37 ℃ and pH 7.0-4.5 at a ratio of 2:1 for 12 days, and the slurry was separated from the water by suction filtration.
The COD production rate of the sludge finally obtained by enrichment under the condition of pH 4.5 is about 13.8mgCOD/L/h, and no methane is generated after 90 days of reaction.
Example 4
10g/L of anaerobic granular sludge is washed for 3 times by clear water and then pretreated for 48 hours under the condition that the BES addition amount is 50 mM/L. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture is up to 5bar, the gas proportion is H 2 :CO 2 The culture was carried out at a rate of 4:1 and maintained at 37 ℃ and a pH of 7.0 to 4.5 for 7 days, and the slurry was separated from the water by centrifugation.
The COD production rate of the sludge obtained by the final enrichment under the condition that the pH value is 4.5 is about 21.4mgCOD/L/h, and no methane is generated after 90 days of reaction.
Example 5
After washing 10g/L anaerobic flocculent sludge with clear water for 3 times, pretreatment is carried out for 24h under the conditions that the pH value is 3.0 and the BES adding amount is 25 mM/L. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture is 2bar, the gas proportion is H 2 :CO 2 The culture was carried out at a rate of 4:1 and maintained at 37 ℃ and a pH of 7.0 to 4.5 for 15 days, and the slurry was separated from the water by centrifugation.
The COD rate of the sludge finally obtained by enrichment under the condition of pH 4.5 is about 27.3mgCOD/L/h, and no methane is generated after 90 days of reaction.
Example 6
After washing 10g/L anaerobic flocculent sludge with clear water for 3 times, pretreatment is carried out for 24h under the conditions that the pH value is 3.0 and the BES adding amount is 25 mM/L. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture is 2bar, the gas proportion is H 2 :CO 2 The culture was carried out at 37 ℃ and pH 7.0-4.5 at 4:1 for 15 days, and the slurry was centrifuged.
The COD yield rate of the sludge obtained by the final enrichment under the condition of pH 5.5 is about 31.4 mgCOD/L/h.
Comparative example 1
The homoacetogenic sludge enrichment was performed with reference to the method described in example 5, but with the environmental pH maintained at 7.0, the specific protocol was as follows:
after being washed 3 times by clear water, 10g/L anaerobic flocculent sludge is pretreated for 24h under the conditions that the pH value is 3.0 and the BES addition amount is 25 mM/L. Then the sludge is placed in a fermentation tank, and H is introduced 2 And CO 2 The gas mixture of (2) to 2bar, the gas ratio is H 2 :CO 2 (iii) 4:1, andthe culture was carried out at 37 ℃ and pH 7.0 for 15 days, and the slurry was separated by centrifugation.
The final sludge enriched at pH 7.0 has COD rate of about 8.3mgCOD/L/h, and after 33 days of reaction, 31% methane is present in the tail gas, and further sludge treatment is needed to inhibit methane generation.
As can be seen from the above examples and comparative examples, the scheme of the invention can finally realize the continuous operation of the homoacetogenesis process, the stable production of carbon source and the realization of CO through the pH regulation and control of the acclimation environment 2 And (4) recycling.
Claims (10)
1. A method for promoting synthesis of a carbon source in a homoacetogenic process through large-scale sludge acclimation is characterized by comprising the following steps:
1) anaerobic sludge of a sewage plant is taken as a raw material, the anaerobic sludge is washed by clear water, and non-acid-producing strains such as methanogens and the like in the sludge are inhibited through pretreatment;
2) placing the anaerobic sludge obtained in the step 1) in a fermentation tank, introducing a mixed gas of hydrogen and carbon dioxide into the fermentation tank, and continuously fermenting at the temperature of 25-50 ℃; controlling the pH value to be kept stable in the fermentation process, wherein the stable pH value is between 4.5 and 7.0, and the pH value is controlled to be 7.0 in an initial sequencing batch mode;
3) taking out the sludge used in the step 2) after 7-30 days of the sequencing batch, and separating to obtain an aqueous solution containing a carbon source and sludge enriched with homoacetogenic bacteria;
4) the sludge can be subjected to the steps 2) and 3) again, wherein the stable pH is reduced by 0.5 compared with the previous batch until the stable pH is 4.5 and then is kept stable, so that the system can inhibit the generation of methane and keep the system to stably produce the carbon source without other treatment conditions.
2. The method for large-scale sludge acclimation to promote synthesis of carbon source in the homoacetogenic process according to claim 1, wherein the anaerobic sludge in step 1) is any one of anaerobic granular sludge or anaerobic flocculent sludge; preferably the sludge is anaerobic flocculent sludge; the sludge concentration is between 5 and 60 gMLSS/L.
3. The method for large-scale sludge acclimatization to promote synthesis of carbon source in the homoacetogenic process according to claim 1, wherein the pretreatment manner in step 1) is one or more of heat treatment at 105 ℃, acid treatment at pH 3.0, alkali treatment at pH 11 and 0-100mM/L BES treatment, wherein the preferred pretreatment manner is acid treatment at pH 3.0, 0-100mM/L BES treatment and 0-100mM/L BES treatment at pH 3.0, and most preferred manner is addition of 0-100mM/L BES treatment at pH 3.0.
4. The method for large-scale sludge acclimatization to promote synthesis of carbon source in the homoacetogenic process according to claim 3, wherein the treatment time is 0-72h, and preferably 24-48 h.
5. The method for large-scale sludge acclimatization to promote synthesis of carbon source in the homoacetogenic process according to claim 3, wherein the BES is added in an amount of 0-100mM/L, preferably 50 mM/L; the BES addition amount is 0-100mM/L, preferably 25mM/L, in conjunction with BES treatment at pH 3.0.
6. The method for large-scale sludge acclimatization to promote synthesis of carbon source in the homoacetogenic process according to claim 1, wherein the fermentation environment in the step 2) is an anaerobic fermentation environment, the anaerobic atmosphere is a mixed atmosphere of hydrogen and carbon dioxide, and the air pressure is maintained between 0 and 2 bar.
7. The method for promoting synthesis of carbon source in the process of homoacetogenesis according to claim 6, wherein H is contained in the atmosphere 2 :CO 2 H is preferably selected from 8:1 to 1:1(v: v) 2 :CO 2 ＝4:1。
8. The method for large-scale sludge acclimatization for promoting synthesis of carbon source in the homoacetogenic process according to claim 1, wherein the environmental temperature in the fermentation process of step 2) is controlled between 25 ℃ and 50 ℃, preferably 37 ℃.
9. The method for large-scale sludge acclimatization to promote synthesis of carbon source by homoacetogenic process according to claim 1, wherein the sequencing batch time in step 3) is 7-30 days, preferably 15 days.
10. The method for large-scale sludge acclimation to promote synthesis of the carbon source in the homoacetogenic process according to claim 1, wherein the sludge-water separation manner in the step 3) is one or more of centrifugation, natural sedimentation, filter pressing and suction filtration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210450992.2A CN114934078B (en) | 2022-04-27 | 2022-04-27 | Method for synthesizing carbon source in process of promoting homoacetogenesis by large-scale sludge domestication |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210450992.2A CN114934078B (en) | 2022-04-27 | 2022-04-27 | Method for synthesizing carbon source in process of promoting homoacetogenesis by large-scale sludge domestication |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114934078A true CN114934078A (en) | 2022-08-23 |
| CN114934078B CN114934078B (en) | 2024-04-02 |
Family
ID=82863000
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210450992.2A Active CN114934078B (en) | 2022-04-27 | 2022-04-27 | Method for synthesizing carbon source in process of promoting homoacetogenesis by large-scale sludge domestication |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114934078B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433278A (en) * | 2011-12-13 | 2012-05-02 | 江南大学 | Enrichment culture method of homoacetogenic bacteria |
| CN104263764A (en) * | 2014-09-15 | 2015-01-07 | 常州大学 | Process for high-efficiency anaerobic production of acetic acid with homoacetogenic bacteria-rich seed sludge |
| CN110396528A (en) * | 2019-07-30 | 2019-11-01 | 山东众森固废资源循环利用研究院有限公司 | A kind of method of sludge anaerobic microbe conversion synthesis gas beam system acetic acid |
-
2022
- 2022-04-27 CN CN202210450992.2A patent/CN114934078B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102433278A (en) * | 2011-12-13 | 2012-05-02 | 江南大学 | Enrichment culture method of homoacetogenic bacteria |
| CN104263764A (en) * | 2014-09-15 | 2015-01-07 | 常州大学 | Process for high-efficiency anaerobic production of acetic acid with homoacetogenic bacteria-rich seed sludge |
| CN110396528A (en) * | 2019-07-30 | 2019-11-01 | 山东众森固废资源循环利用研究院有限公司 | A kind of method of sludge anaerobic microbe conversion synthesis gas beam system acetic acid |
Non-Patent Citations (4)
| Title |
|---|
| KOK S D等: "Impact of dissolved hydrogen partial pressure on mixed culture fermentations", APPLIED MICROBIOLOGY&BIOTECHNOLOGY, no. 97, pages 2617 - 2625 * |
| ZHANG F等: "In situ hydrogen utilization for high fraction acetate production in mixed culture hollow-fiber membrane biofilm reactor", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, no. 97, pages 10233 - 10240 * |
| 李冬娜等: "污泥厌氧发酵产酸机理及应用研究进展", 生物质化学工程, vol. 54, no. 2, pages 51 - 60 * |
| 谢丽等: "同型产乙酸菌研究进展及其环境生物技术应用", 同济大学学报(自然科学版), vol. 46, no. 1, pages 67 - 73 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114934078B (en) | 2024-04-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Van Zyl et al. | Design and start-up of a high rate anaerobic membrane bioreactor for the treatment of a low pH, high strength, dissolved organic waste water | |
| WO2015000266A1 (en) | Enhanced sewage biological nitrogen and phosphorus removal method based on polyhydroxyalkanoates metabolic regulation | |
| CN110734933A (en) | Method for improving medium-chain fatty acid yield of anaerobic fermentation of waste activated sludge | |
| CN108383239B (en) | Integrated biological treatment process for shortcut nitrification anaerobic ammonia oxidation and phosphorus removal under intermittent aeration mode | |
| CN110656133B (en) | Pretreatment method for promoting production of medium-chain fatty acid by anaerobic fermentation of waste activated sludge | |
| CN107973404B (en) | Method for producing acetic acid by directional fermentation of organic waste through regulation and control of redox mediator and coupling low-temperature denitrification | |
| KR100853287B1 (en) | High rate methanc production system using anaerobic archaea | |
| CN116732111A (en) | Method for promoting conversion of waste biomass energy by adopting persulfate oxidation and sulfate reduction bacteria novel system | |
| CN112279375A (en) | Domestication method of denitrification synchronous denitrification dephosphorization bacteria | |
| CN109574434B (en) | Method for producing methane by enhancing anaerobic digestion of excess sludge by using alkaloids | |
| CN114934078B (en) | Method for synthesizing carbon source in process of promoting homoacetogenesis by large-scale sludge domestication | |
| CN105110590A (en) | Method and device for intensifying high-temperature hydrolysis acidification of excess sludge from sewage treatment plant through aeration | |
| CN115432805A (en) | Method and device for realizing deep denitrification and desulfurization of fermentation wastewater by virtue of short-cut nitrification synchronous anaerobic ammonia oxidation coupled sulfur autotrophic denitrification | |
| CN205024060U (en) | Sewage treatment plant excess sludge high temperature hydrolytic acidification device is reinforceed to aeration | |
| CN105060623B (en) | Double dirt method for treating water based on hydrogenesis and acetogenesis/hydrogen autotrophic denitrification coupling | |
| KR101683271B1 (en) | Apparatus for processing waste water with algae | |
| JPH08294396A (en) | Production of hydrogen gas | |
| CN108249725B (en) | Method for producing methane by pre-fermenting and enhancing anaerobic digestion of excess sludge | |
| CN114873725B (en) | Device and method for realizing fermentation type short-cut denitrification dephosphorization | |
| CN111003881A (en) | Method for recycling food waste | |
| KR20210026556A (en) | Denitrification apparatus for sewage supplied with carbon source using sewage sludge and method for reducing nitrous oxide using the same | |
| CN217733073U (en) | Homoacetogenic continuous production test device | |
| CN107162314B (en) | Method for treating excess sludge and recycling resources and method for improving VFAs yield | |
| CN113736833B (en) | Method for producing volatile fatty acid by utilizing orange peel | |
| CN112661376B (en) | Municipal sludge pretreatment method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |